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دراسة نسيجية لجلد اناث وذكور الفئران بعد اعطاء عقار الكلوكوزامين سلفيت == Histological Study On The Male And Female Mice's Skin After The Administration Of Glucosamine Sulphate

Author name: معن ماجد صالح السامرائي
Supervisor name: عبد الامير حمادي النعيمي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
Key words:
  • Skin
  • glucosamine sulphate
  • glycosaminoglycans
First pages:
Abstract: يتكون الجلد وكما هو معروف جيدا من عدة طبقات من الخلايا متجددة باستمرار عبر اعادة بناء نفسها ذاتيا مرة كل ثلاثون يوما من خلال دورة حيلتية متكررة وتقسم هذه الطبقات الى نوعين اساسية البشرة والادمة. يعتبر الكلوكوز امين سكر اميني بسيط ينبعث من عمليات الايض ال | It is well known that the skin is made up of multiple layers of cells that fare constantly going through self shedding and regeneration once every 30 days, or so in repeated cycles. The layers can be broadly divided into two sections : the epidermis and the underlying dermis. Glucosamine is an amino monosaccharide derived from cellular glucose metabolism. Glucosamine is a simple component or "building block" of more complex molecules. Glucosamine was considered to be an effective treatment for many joint diseases especially osteoarthritis. It is believed that glucosamine maintain healthy joint functions and rebuild damaged joint cartilage, tendons, ligaments and other connective tissue. It does this by stimulating the production of glycosaminoglycans (GAG’s) which are the structural components of cartilage and connective tissue else where in the body. On that basis, this study was designed to study the effects of glucosamine sulphate on the skin.Twenty male and twenty females adult mice (weighting between 23.3gm to 27.2gm), were divided into two equal subgroups (control and experimental); the drug was given at noon three hours after starvation it had been grinded and mixed with food and given as a single oral dose of 350mg/kg body weight per day for 35 successive days. Histological examination and statistical analysis of multiple sections of ventral and dorsal skin of male and female mice and of both subgroups were done by using hemotoxyline and eosin stain. Special stains were used to demonstrate the specific components of the skin as follows : for collagen fibers (Van Gieson's staining method), for elastic fibers (modified Taenzer - Unna Orcien staining method), and for ground substance (Alcian blue stain method).The results reveal that there was no particular change in the epidermis between the experimental and control groups. On the contrary there was a marked thickening with high cellular contents of both layers of the dermis (papillary and reticular).The dermis of the treated animals contains more collagen and elastic fibers. The predominant cell of the dermis was the fibroblast which was more obviously seen in the ventral skin of the treated animals. This study confirms practically that glucosamine sulphate induces significant structural changes in the skin of male and female mice. Using glucosamine sulphate clinically for medical conditions rather than arthritic diseases is now the target of most recent researches, its ability to decrease wrinkles in the aged skin and promotion of wound healing with less scar tissue is consider to be a light for dermatologist and plastic doctors, plus its effect to rebuild any aged, injured and diseased tissue in the body.

دراسة تاثير اديبونكتين على هيكل كبد الفئران وعلاقته بالنظام الغذائي وكمية الدهون في الجسم == A Study Of Adiponectin Effects On The Mice Liver Architecture In Relation To Dietary Intake And Body Fat Content

Author name: غسان علي عبد الحسین
Supervisor name: سلمان شفیق سلمان | عماد غانم قاسم
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: يعتبر مرض الكبد الدهني غير الكحولي الاضطراب المزمن الاكثر شيوعا في الكبد واصبح تشخيصه في ازدياد مع زيادة وباء السمنة العالمي وقد تم تصميم الدراسة الحالية لدراسة الفوائد المحتملة للاديبونيكتين على النماذج الحيوانية من مرض.تم تطوير نموذجين من اضطراب الكبد | Non - alcoholic fatty liver disease is the most common chronic liver disorder whose prevalence has been on the rise with the worldwide increasing pandemic of obesity. The current study was designed to study potential benefits of the adipokine (adiponectin) on animal models of non - alcoholic fatty liver disease.Two models of the disorder were developed using two different approaches. In experiment 1 (25 mice, last 8 weeks), fatty change was induced using high fructose intake to simulate human - like diet induced obesity and fatty liver disease. In experiment 2 (25 mice, last 3 weeks), fatty liver damage was induced by using methionine - choline deficient diet. Animal body weights, absolute and relative liver weight, food consumption and fructose water consumption were recorded. Visceral (epididymal) fat and liver tissue were studied using routine hematoxylin and eosin staining and histochemical stains including Masson trichrome, Periodic acid schiff and reticulin stains. Morphological and morphometric changes were compared between animals treated with adiponectin, untreated diseased animals, and control healthy animals and a non - alcoholic steatosis activity score was used to evaluate the severity of liver damage. Random blood sugar and serum lipid levels were also included as biochemical metabolic parameters.High fructose intake resulted in larger visceral fat depots and weight gain, effects that were combated by adiponectin treatment despite increased food intake. Hepatic steatosis, inflammation, pyknotic pictures and fibrosis were reduced in adiponectin treated animals resulting in a lower activity score. Random blood sugar levels were normal in all animal groups but were significantly lower in adiponectin treated groups. TG and VLDL were alleviated with adiponectin treatment.Methionine - choline deficient diet resulted in emaciation of animals, much reduced visceral fat depots but a more severe form of liver fatty damage over a shorter period of time when compared to high fructose obese animal models. Weight loss was worsened after adiponectin treatment. Hepatic steatosis, inflammatory foci, ballooning degeneration and fibrosis were all exaggerated but remained significantly less severe with less activity score in adiponectin treated animals. Random blood sugar levels were normal in all animal groups but were significantly higher after adiponectin treatment. TG and VLDL was alleviated with adiponectin treatment.Adiponectin affects appetite, food consumption and body composition in healthy animals and animal models of non - alcoholic fatty liver disease through different mechanisms. It has beneficial effects in reducing body weight in obese animals but can modulate weight loss in lean animals on methionine - choline deficient diet. Adiponectin has autocrine/paracrine effects on visceral adipose tissue and can correct the dyslipidemic picture of fatty liver disease. The antisteatotic and antifibrotic effects of adiponectin carry greater therapeutic potentials in obesity - related liver disease.

هرمون اللبتين والاستقلاب : دراسة كيميانسيجية وكيمياحيوية شاملة في الحيوانات الطبيعية والمصابة بالسكري == Leptin And The Metabolism : A Comprehensive Histochemical And Biochemical Study In Healthy And Diabetic Animals

Author name: سامح سمیر موسى عكیلة
Supervisor name: سلمان شفیق سلمان | سامیة عباس علیوي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: The adipocytokine leptin is a major player in the pathogenesis of diabetes mellitus and obesity. Since its discovery around 20 years ago, leptin has been; and still is; the subject of extensive research.The current study was designed to examine the effects of physiological doses of leptin on major organs involved in metabolism. The anatomical, histochemical, physiological, and biochemical effects of leptin were studied in healthy animals and in animal models of type 1 and type 2 diabetes.Eighty male albino mice were divided into three groups. The first group (A) consisted of healthy non - obese non - diabetic animals, a subgroup (A1) of which received leptin therapy to study its effects under normal conditions without metabolic disturbances. In the second group (B), a model of diet induced obesity and insulin resistance was developed using a combination of high fat diet and low dose streptozocin injection. In one subgroup (B1), the effects of leptin were studied under dietary manipulation after the discontinuation of high fat diet. In other subgroups, leptin effects were examined with continuous HFD with (B3) and without (B2) concomitant metformin therapy. The third group animals (C) represented a type 1 DM model developed by high dose streptozocin injection. A subgroup (C3) served as model control, not receiving any hormonal therapy. The others two subgroups received leptin treatment with (C2) and without (C1) concomitant insulin therapy.Statistical parameters for the study included the body weight, food and caloric consumption, adiposity index and specific organ weights and weight ratios. The epididymal fat pad, liver and pancreas were examined for gross anatomical and histological changes. Adipose tissue and some pancreatic sections were stained with H&E, liver sections were stained with periodic acid schiff stain, other pancreatic sections were stained with modified Gomori's aldehyde fuchsin. Random blood sugar and serum lipid levels and insulin tolerance test were also studied as biochemical parameters.Leptin therapy in healthy non - obese animals resulted in a reduction in food consumption, body weight, adiposity index and white fat pads weight. It also caused a reduction in the diameter and surface area of epididymal adipocytes. Serum TGL and LDL levels were significantly reduced. iiiThe same effects were seen in group B animals that underwent dieting or received metformin, but not in ones receiving leptin alone. Animal models of type 1 DM showed a moderate response to leptin therapy alone but the response was dramatically enhanced when treatment was combined with insulin. Sever weight loss and polyphagia of type 1 DM were greatly ameliorated by leptin therapy and there was an improvement in fat pad weights and adipocyte measurements. The hepatic glycogen content and insulin sensitivity were greater in animals treated with leptin (A1), on diet and leptin (B1) or metformin (B3) and in animals on leptin with insulin (C1). This was associated with significantly lower levels of random blood sugar. The pancreatic islet surface area was markedly reduced while the ? - cell/ ? - cell ratio was increased in treated animals.Leptin therapy can alter body weight and adiposity index by affecting appetite and food consumption via central and peripheral mechanisms involving the control of feeding behavior and manipulating the processes involved in carbohydrate and lipid metabolism. It can also correct the metabolic disturbances of type 2 DM but requires the concomitant use of metformin and/or the manipulation of dietary content of fat and carbohydrates. Much of its influence arises from its ability to enhance insulin sensitivity.Leptin also has a sparing effect on hepatic glycogen, favoring in turn the use of fatty acids for energy expenditure. Leptin effects on blood glucose are insulin dependent in most cases but can be insulin - independent in type 1 DM by utilizing alternative metabolic pathways for energy expenditure and interacting with hyperglycemia - inducing hormones.

ظهور المؤشرات في الثدي الطبيعي المحيط بسرطان الثدي في عينة من النساء العراقيات

Author name: احمد فاخر حميد
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

التعدد الشكلي للمستقبل الثاني لعامل الورم التنخري والجين المحفز للـ CD4 والانترلوكين 37 في تقييم التهاب المفاصل الرثوي == Tumor Necrosis Factor Receptor II, Cd4 Enhancer Gene Polymorphisms And IL - 37 In Assessment Of Rheumatoid Arthritis

Author name: وسناء جمعة محمد
Supervisor name: محمد شمخي جبر | باسم شهاب احمد
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: التهاب المفاصل الرثوي (Rheumatoid Arthritis) مرض جهازي التهابي مزمن يصيب المفاصل وتحديدا الاغشية الزلالية والتراكيب المفصلية، والذي يحدث للعديد من الناس وبنسبة تقارب 0.5 الى 1% من السكان في العالم. لوحظ خلال الدراسات الوبائية لمرض التهاب المفاصل الرثوي | Rheumatoid arthritis (RA) one of the most common systemic autoimmune diseases characterized by chronic joint inflammation and subsequent joint destruction. To date, it well known that RA is characteristic of the expansion of the synovium and infiltration of the inflammatory cells coupled with destruction of adjacent articular cartilage and bone. This is strongly dependent on CD4 T cell. CD4 Cells stimulate monocytes, macrophages, synovial fibroblasts, and other cells to produce cytokines such as tumor necrosis factor - ? (TNF - ?, interleukin - 1 (IL - 1), IL - 6, IL - 15, IL - 17 and metalloproteinases that produce tissue damage. TNF is a major inflammatory cytokine contributing to the pathogenesis of RA, which provides rational for development of anti - TNF biological agents in the treatment of RA.Recently have shown that IL - 37 is a key cytokine in regulating inflammatory response, mainly by inhibiting the expression, production, and function of proinflammatory cytokines. Objectives This study planned to evaluate the association of TNFRII and CD4 enhancer genes polymorphisms in development and severity of RA in Iraqi patients, evaluate IL - 37 in patients with RA and investigate the correlation between IL - 37 levels with disease activity and relation of inflammatory parameters (ESR, CRP, ACPA, and RF) with TNFRII, CD4 enhancer genes polymorphisms.Patients and methods This study was performed during the period from May to September 2015. The patients were attending the out patients' Clinic in Medical City/Baghdad Teaching hospital/rheumatology unit and the laboratory ELISA tests were done in nursing home hospital laboratory, polymerase chain reaction (RFLP) analysis was performed in the specialist private molecular laboratory (ASCO Lab) in Al - Harthia / Baghdad. Fifty patients and 50 apparently healthy control individuals, Patients received disease modifying anti rheumatic drugs (DMARDs) and newly diagnosed patients.The diagnosis of RA patients has performed under the supervision of rheumatic disease consultant physician at the consultation clinic of Baghdad teaching hospital of the medical city. Blood samples had taken from RA patients to measure (ESR), white blood cells count and hemoglobin. Also, analyze the serum levels of ACPA, RF screen CRP and estimate the levels of IL - 37 in patients and healthy individuals using ELISA test kits. TNFRII and CD4 enhancer genes polymorphisms genotyping had achieved by polymerase chain reaction restriction fragment length (PCR - RFLP).Results The results showed the estimation of RF by screening test revealed that its mean level was high among RA patients (168.87+31.62) in comparison with apparently healthy control (4.96+0.71) with highly significant difference (p<0.01). There was a higher positivity of Anti CCP in the patients sera (114.85+21.06) in comparison with apparently healthy control (1.71+0.13) (P<0.01). while the estimation of CRP showed that its level was higher among sera of RA patients (38.39+4.31) in comparison with healthy control group (16.49+2.51) (P<0.01). Furthermore, the ESR level in RA patients were higher than apparently healthy individuals (52.96+3.68) and (10.44+0.74) respectively with significance (P<0.01).The frequencies of the MM, MR, RR genotypes of TNFRII gene polymorphisms were 60%, 32%, 8% in RA patients and 52%, 42%, 6% in controls. There were no significant differences in the genotypes frequencies polymorphisms of the TNFRII 196 MR polymorphism between apparently healthy control and RA. The frequencies of AA, AG, GG genotypes of CD4 10845 A/G in patients was 36%, 42%, 22% and 8%, 44%, 48% in control group. There were significant differences in the genotypes frequencies polymorphisms of the CD4 10845A/G polymorphisms between apparently healthy control and RA patients (p<0.01). AA genotype were significantly more likely to develop RA (OR=1.355). There was significant increase in disease activity and severity in patients carries AA genotype (p<0.01).IL - 37 levels were elevated markedly in RA patients (101.31+10.41) compared with apparently healthy control (43.90+0.91) (p<0.01). More importantly, IL - 37 showed a significant correlation with disease activity (CRP) in RA patients (p<0.05). Also, IL - 37 show non - significant relationship with (MM, MR, RR) exon6 TNFRII in RA patients compared with controls, and non - significant difference between level of IL - 37 with CD4 10845 A/G genotypes compared with healthy control.Conclusion All findings suggested that TNFRII - 196R genotypes not associated with RA diagnosis. In addition, genetic polymorphisms at the CD4 enhancer gene are one of important factors that associated with susceptibility and severity of RA and can serve as a genetic marker for the risk of development of RA.

دورا محتملا لفيروس ابشتاين بار في عملية تسرطن الغدة الدرقية الحليمية == A Possible Role Of Epstein - Barrvirus In Carcinogenesis Of Papillary Thyroid Carcinoma

Author name: هند علي خميس
Supervisor name: عامر رشيد النجار | سعد حسن محمد علي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: This study was designed as a retrospective research. A total number of (90) formalin - fixed, paraffin embedded tissues were studied. Malignant and normal thyroid tumors tissue blocks were collected from the archives of histopathology laboratories of different general hospital including ALYarmouk Teaching Hospital (Baghdad), Baghdad Medical City Teaching Hospital as well as many private laboratories in Baghdad, during the period from May 2013 to May 2014. These cases include ? Thirty tissue specimens from patients with papillary thyroid cancer. ? Thirty tissue specimens with benign thyroid lesions as control thyroid tissues group. ? Thirty (30) thyroid specimens were obtained from the normal thyroid tissues from the same those totally - thyroidectimized patients for thyroid papillary cancer (PTC) and have served as the second control group for this study. This study was therefore a paired case - control design. The sections were stained by hemotaxyline and eosin staining so as to confirm the diagnosis and assure that the intervening sections ( that were specified for the in situ hybridization and Immunohistochemistry study have containing the questioned tissues from papillary thyroid cancers and benign thyroid tissues (control group).After histopathological sectioning of these thyroid gland tumors and staining by Hematoxylin and Eosin, a final definitive diagnosis was done by histopathologist consultant. The practical part of this study was designed in four pathways : 1. Molecular detection of Epstein - Barr virus in those tissue blocks were performed by using ultra - sensitive version of in situ hybridization (ISH) for detection of EBV - EBERs. 2. To perform immunohistochemical study to demonstrate EBV - latent gene (LMP1, EBNA - 2) in the papillary thyroid cancer compared to control group. 3. To perform immunohistochemical screening study to demonstrate the over expression state of p53 - tumor suppressor gene in those tissues with papillary thyroid cancer compared to control group. 4. To perform immunohistochemical screening study to detected the CD8 and CD56 in tissues with papillary thyroid cancers compared to control group. The obtained results are summarized as follows : 1. The mean age of patients with papillary thyroid cancer and simple nodular goiter was (39.87&41.13) years respectively, with standard deviation ±(11.773,9.243) years. 2. In this study, the percentage of the females with papillary thyroid cancer was higher (86.7%; 26 cases) than the percentage of their PTC - male counter parts (13.3%; 4 cases). The patients whom sub totally thyroidectimized for simple noduler goiters , the percentage of females was also higher (73.3%; 22 cases) than the percentage of their male counter parts with simple noduler goiters ( 26.7%; 8cases). Female / male ratios of the patients with PTC and simple noduler goiters were 6.5 and 2.75 respectively. 3. The percentage of EBV results in the total group of PTC was (50%) while the percentage in the total group of normal thyroid tissue was (26.7%) and lastly in the total simple nodular goiter group was (10.0%). 4. The positivity rate of EBV EBERs - ISH technique in the total group of PTC was (30%), where as its percentage in NTT tissues was (16.7%) and in SNG tissues was (3.3%). 5. The percentage of positive - EBNA - 2 IHC technique in the total group of PTC was (20%), where as its percentage in NTT tissues was (10.0%) and in SNG tissues was (6.7%). 6. The percentage of positive - EBV - LMP - 1 - IHC technique in the total group of PTC was (43.3%), where as its percentage in NTT tissues was (20.0%) and in SNG tissues was (6.7%). 7. Mutated P53 - protein was detected in 28 cases (70%) of the studied cases. Twelve cases (30.0 %) showed negative IHC reactions. 8. The highest total percentage of CD8 - IHC reactions (21 cases; 70%) was found in those with papillary thyroid cancer followed by normal thyroid tissues cases ( 5 cases; 16.7%) and then ( 2 cases : 6.7%) in simple nodular goiter. 9. It was found that the highest percentage of CD56 - IHC reactions was in those with normal thyroid tissues (25 cases; 83.3%) followed by an equal percentages of CD56 - IHC reactions in either thyroid papillary or simple nodular goiter (2 positive cases; 6.7%, each). 10. The importance of CD56 marker has been found to play a role or it may be better to be used as a negative - diagnostic biomarker for papillary thyroid cancer in differentiating it from other malignancies as well as benign lesions of the thyroid gland, individually as well as in combination with other markers for clinical evaluation of those patients. 11. The evident high mutated p53 - over expression, as reflected by abnormal gene product, among papillary thyroid cancer patients indicates for a pivotal role of such genetic mutation in their carcinogenesis as well as could be useful in the clinical evaluation of patients with papillary thyroid cancer, too. 12. The high coexistence of p53 - mutation with EBV in PTC could point for participation of EBV - transformation genes in the p53 activation in high proportion of PTC. 13.The high percentage of CD8 lymphocytes in EBV - positive PTC as compared to their EBV - negative counterparts could reflect the participation of specific cellular immunity against both, the papillary thyroid cancers and this EBV infection during the initiation and progression of EBV - associated papillary thyroid cancers.

تشخيص مصلي وجزيئي لفيروس الحلا البشري النوع السادس المصاحب لبعض سرطانات الدم == Serological And Molecular Detection Of Human Herpesvirus Type 6 Associated With Certain Hematologic Malignancies

Author name: هديل محمد فياض
Supervisor name: علاء فاضل علوان | داود سلمان داود
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Human herpesvirus 6 (HHV - 6) is largely ubiquitous double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus.HHV - 6A and HHV - 6B infects very high percentage of population around the world, primarily during childhood through respiratory droplets. HHV - 6 has been found out in many types of cancers : lymphomas, leukemia, cervical cancer, and brain tumors.This cross - sectional case control study was carried out in Baghdad Teaching Hospital and National Center of Hematology - AL - Mustansiriyah University - Baghdad, for the period from 1 September 2013 to 1 April 2015.The aims of the study were to explore the seropositivity rate of HHV - 6 antibodies among Iraqi patients with different hematological malignancies using different laboratory assays, beside, the molecular determination of plasma viral DNA load by quantitative polymerase chain reaction (qPCR).The study included 109 patients with different hematological malignancies; 24 patients with acute lymphoblastic leukemia (ALL), 30 patients with acute myeloid leukemia (AML), 11 patients with Hodgkin lymphoma (HL), 39 patients with non hodgkin lymphoma (NHL) and 5 patients with chronic lymphocytic leukemia(CLL). All patients were newly diagnosed and enrolled before receiving chemotherapy. The age range was between 14 - 80 years. Fifty three (48.6%) and 56 (51.4%) patients were males and females, respectively. The diagnosis of their malignancies was based on either bone marrow aspirate biopsy and lymph node biopsy as well as cytochemical investigations. Additionally, 59 apparently healthy blood donors were enrolled as control group. The age range was between 18 - 59 years, twenty eight (47.4%) and 31 (52.6%) were males and females, respectively.The serum anti - HHV - 6 IgG was detected by indirect immunofluorescent technique (IFAT) as well as by enzyme linked immunosorbant assay (ELISA).While, the serum anti - HHV - 6 IgM was detected by ELISA only. The detection and quantification of plasma viral DNAemia was carried out by quantitative polymerase chain reaction. Serological as well as molecular assays were done in Central Public Health Laboratory in Baghdad. The highest IFAT positivity rate was among patients with CLL (100%), followed by patients with AML (83.3%), and the least positivity rate was among patients with NHL (64.1%) compared to that of healthy control. Generally, the total anti - HHV - 6 IgG by IFAT was insignificantly higher among patients compared to healthy controls (74.3% vs 61.0%, p=0.074). The anti - HHV - 6 IgG positivity rates by ELISA were insignificantly higher in all groups of haematological malignancies except in patients with AML in whom it was significantly higher compared to healthy controls (96.7% vs 72.9%, p= 0.007 ). A part from AML, the highest anti - HHV - 6 IgG by ELISA was among patients with ALL (87.5%) followed by patients with NHL (84.6%), and the least was among patients with CLL (80.0%) compared to healthy control. Generally, the total anti - HHV - 6 IgG positivity rate detected by ELISA was significantly higher compared to IFAT in patients (p= 0.013) as well as in healthy control (p= 0.0005).The results of anti - HHV - 6 IgM positivity rate as detected by ELISA was significantly higher in patients with AML (36.7%, p=0.0004), and patients with HL (27.7%, p= 0.038) compared to healthy controls (6.8%). Whereas, other groups of hematological malignancies showed insignificantly higher positivity rate versus control group. However, the highest positivity rate was among patients with AML (36.7%), and the least positivity rate was among patients with CLL (0%). Generally, the total ELISA anti - HHV - 6 IgM positivity rate was significantly higher in patients versus healthy controls (22.0% vs 6.8%, p= 0.011). The results of PCR showed that 5 out of 109 patients had detectable HHV - 6 DNA in the plasma. The highest detection rate was among patients with HL (27.3%), followed by patients with ALL (4.2%), and then patients with AML (3.3%), while the virus was not detected in other disease categories as well as in healthy controls. The plasma viral load (mean ± SD) among the three Hodgkin lymphoma patients was 1.4± 0.3*102 particle/milliliter.The study concluded that the seropositivity rate of HHV - 6 infection is highly prevalent among Iraqi patients with hematological malignancies compared to healthy population. However, primary or reactivation infection as determined by anti - HHV - 6 IgM in patients as well as healthy individuals is relatively low. Furthermore, the HHV - 6 DNA detection rate as well as plasma viral load among malignant patients before receiving immunosuppressive treatment were low.

دراسة Toll like receptors(TLR2& ( 4 ومؤشرات حيوية مختارة في المرضى المصابين بسرطان المثانة == Study Of Toll Like Receptors (TLR - 2 And 4) And Selected Biomarkers In Bladder Cancer Patients

Author name: هدى سعدون البياتي
Supervisor name: ميسون علي سليم | ناهي يوسف ياسين | عصام سلمان العزاوي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Worldwide, cancers of the urinary bladder are complex and involve genetic abnormalities and may be due to different environmental chemical carcinogens, as well as chronic infection like Schistosomiasis, all these will allow normal transitional cells to become cancerous which are typically transitional cell carcinoma(TCC).This study was conducted at AL - Yarmouk and Baghdad Teaching Hospitals in Baghdad from June 2013 to April 2014, of which urine and bladder tissue were taken from 57 bladder cancer patients and 80 controls to assess Interleukin - 8 (IL - 8), Matrix MetalloPeptidase - 9 (MMP - 9), Bladder Cancer - Associated Protein (BLCA - 4),Toll - Like Receptors (TLR - 2and TL - R4) level among them and to evaluate their roles in cancer development.One hundred thirty seven Iraqi individual were divided to 28(20.43%) newly diagnosed and 29(21.16%) recurrent bladder cancer patients (relapse), 40(29.19%) apparently healthy volunteers and 40(29.19%) autopsies (apparently normal urothelium), of whom urinesample and tissue biopsy were collected, clinical diagnosis of patients carried out by cystoscopic and histopathological examination, the mean ±SD age of bladder cancer patients were (63± 9.3) with (M : F ratio7.1 : 1).Predominant cancer type was Transitional cell carcinoma (TCC). According to risk factors of bladder cancer, out of 57 bladder cancer patients, 34(59.64%), 29(50.87%), 9(15.78%), 7(12.28%), 6(10.52%) were smoker, had UTI, history of Schistosomiasis, family history ofcancer and stones respectively. In this study 30 patients had non - muscle invasive bladder cancer, most of them (21) had newly diagnosed tumors, and only 9 had recurrent disease, as well as, 27 patients with muscle - invasive bladder tumor, just 7 had newly diagnosed tumors and 20 with recurrent disease.According to the result of ELISA technique, the mean±SD urine level IL - 8(pg/ml) for recurrent bladder cancer patients was 359.6±170.1pg/ml, for newly diagnosed was 265.95±139.09pg/ml and for healthy volunteers was 62.04±37.66pg/ml. The mean ±SD concentration of urine MMP - 9(pg/ml) was 7368.3±1730.19pg/ml for recurrent, 6176.8±2366.9 pg/ml for newly diagnosed and 1131.53±1554.80 pg/ml for healthy volunteers. Mean±SD Level of urine BLCA - 4 (ng/ml) for recurrent was 1.45± 0.26 ng/ml, for newly diagnosed was 1.30± 0.22 ng/ml and for healthy volunteers was 1.02±0.067 ng/ml. Urine level of these markers was significantly higher in recurrence group when compared with newly diagnosed and these levels decreased in normal volunteers which was statistically significance (P<0.001 for IL - 8, MMP - 9and BLCA - 4),also there was a significant increase in urine IL - 8 level according to grade(P=0.0002) and muscle invasion(P= 0.0009) as well as BLCA - 4 showed this significant increase in high grade(P=0.0001), and muscle invasion(P=0.0001),but no significant difference was found in the level of urine MMP - 9 with grade(P=0.23) and muscle invasion(P =0.069)In comparison, IL - 8 and MMP - 9 IHC staining of bladder tissue, showed low expression in cancerous tissue with no significant difference between cancer and normal urothelium(P=0.140 for IL - 8, P=0.265 for MMP - 9 ), and unrelated to grade, muscle invasion and recurrence.Significant increase in TLR - 2 and TLR - 4 expression in bladder cancer tissue than in normal urothelium (P=0.0001 for each), and according to grade and muscle invasion, TLR - 2 showed significant over expression in high grade than low grade (40.7%vs36.6%) with P=0.018, muscle invasion than non - muscle invasion (44.4%vs33.3%) with P value of 0.02, while TLR - 4 was none significantly correlated with grade andmuscle invasion. As well as, there was no correlation between these receptors with tumor recurrence.In regard to risk factors, Smoking, schistosomiasis and family history showed correlation with study marker in different manner as, IL - 8 was significantly highly expressed in smoking associated bladder cancer group, as well as patients with family history of bladder cancer showed high expression IL - 8, for Schistosoma associated TCC patients and UTI they demonstrated a statistically significant high expression of TLR - 2 p= (0.0001).From this study we conclude that urine IL - 8, MMP - 9and BLCA - 4 measured by ELISA showed specificity in diagnosis of bladder cancer, besides that, urine IL - 8, MMP - 9 and BLCA - 4 have a role in discrimination between newly diagnosed vs recurrent, with a significant association between urine IL - 8 with BLCA - 4 (P= 0.0001) and IL - 8 with MMP - 9 (P= 0.005) in patients with recurrent bladder cancer. Urine IL - 8 and BLCA - 4 concentration were statistically with significant increase in high grade than low grade and muscle invasion than non - muscle invasion, On the other hand, bladder cancer cells over express TLR - 2 and TLR - 4,while TLR - 2 showed correlation with high grade and muscle invasion,TLR4 did not show such correlation. Lastly, IL - 8 and MMP - 9 staining showed low expression in tumor tissue of different grades, stages and groups.

دراسة بكتريولوجية على التفاقم الحاد لمرضى الانسداد الرئوي المزمن == Bacteriological Study On Acute Exacerbation Of Chronic Obstructive Pulmonary Disease (COPD) Patients

Author name: هبة اسماعيل علي العزي
Supervisor name: عروبة خالد عباس | عبد الحميد القصير
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: لا يزال مرض الانسداد الرئوي المزمن مشكلة رئيسية في مجال الصحة العامة. ويرتبط التفاقم الحاد مع فقدان سريع في وظيفة الرئة وخلل في نوعية الحياة وهي من الاسباب الرئيسية للامراضية والوفيات في مرضى الانسداد الرئوي المزمن. تعتبر العدوى البكتيريا هي من الاسباب | Chronic obstructive pulmonary disease remains a main problem in public health. The acute exacerbations are related with rapid loss in lung function and defect in the quality of life and are the main causes of morbidity and mortality in COPD. This study was carried out to isolate and identify the bacteria that cause acute exacerbation in chronic obstructive pulmonary disease and sensitivity of isolated bacteria for most of the antibiotics currently in use.Eighty sputum specimens from patients were included in this study and their age was range forty and above , during the period from January 2015 to June 2015 from three places Al - Yarmouk Teaching Hospital, Medical City Hospital and Medical Al khademeain Emamaain City.Bacteria were identified in 52 (65%) while the patient is not specified any reasoned in 28 patients (35%) patients. The diagnosis of bacteria through culture and microscopic examinations and biochemical tests necessary for diagnosis and then the diagnosis has been confirmed of isolation by the Vitek method for each bacterium, the percentages were as follows : Streptococcus pneumoniae was the most frequently isolated bacteria, which recovered from 21 (26.25%) patients. Followed by Klebsiella pneumoniae, Escherichia coli, Pseudomonas spp., which isolated from (7.5%, 7.5%, and 5%) of patients respectively, while (3.75%) for each of Acinetobacter baumannii & Staphylococcus aureus. The proportion of Stenotrophomonas maltophilia was (2.5%) while the proportion (1.25%) for each of Enterobacter cloacae, Leclercia adecarboxylata, Moraxella catarrhalis & Raoultella ornithinolytica.Through the search three cases were infected with Mycobacterium tuberculosis diagnosed by direct Acid Fast Stain test and their percentage was (3.75%).Sensitivity of bacterial isolates to antibiotics Carbapenems and Doxycycline and Chloramphenicol were the most effective against tested bacteria in vitro while Erythromycin, Tetracycline, and Amoxicillin were the least sensitive

دراسة مناعية وجزيئية لخمج القرنية بحمة الحلا - 1 (حمة الهربس البسيط - 1) == Immunological And Molecular Study Of Herpes Simplex Virus - 1 Keratitis

Author name: نهاية كاظم سالم
Supervisor name: عبد العظيم ياسين عبود البراك | فائز اسماعيل الشكرجي
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: يمثل التهاب القرنية (Keratitis) مشكلة كبيرة قد تؤدي الى فقدان البصر احيانا نتيجة لخدش القرنية او فقدان الوضوحية في القرنية بعد اختفاء الالتهاب, ويعتبر فايروس الحلا (الهربس) البسيط - 1Herpes Simplex Virus - 1(HSV - 1))) هو من اهم العوامل المسببة لمرض التهاب ال | Keratitis is a medical term referring to inflammation of the cornea. It is a serious problem, resulting in everlasting loss of vision as a secondary effect to corneal scarring or loss of clarity of the cornea after the inflammation has disappeared. Most common microbes causing viral corneal keratitis is Herpes Simplex Virus - 1 (HSV - 1). HSV - 1 is a double stranded DNA (dsDNA) virus belongs to the Herpesiviridae. It affects the majority of peoples and its infections have been frequently without clinical manifestation. Ocular HSV - 1 infections caused multiple pathologies with possibly the most damaging being herpetic stromal keratitis (HSK). This study aimed to evaluate the abilities of the immunological and molecular indicators to diagnose herpetic keratitis in the infected and contralateral eye of patients, as well as to assess the immune status of both eyes by using tears and corneal scraping. Fifty - two patients with herpetic keratitis involved in this study, they attended the outpatient clinics / Ibn - AL - Haithum Teaching Hospital from March 2014 until December 2014. For the purpose of comparison, thirty, apparently healthy age matched control subjects were also included. Tears were collected from infected eyes of the 52 patients and from 16 out of the 52 patients contralateral eyes as well as from 30 controls. Corneal scraping had been obtained from 16 patient's infected eyes with corneal ulcers (epithelial ulcers) out of the total patients (52) with herpetic keratitis. Indirect immunofluorescence assay technique (IFA) was applied to measure levels of immunoglobulins (sIgA and IgG) in tears specific to HSV - 1. Realtime - polymerase chain reaction RT - PCR test was used to diagnose HSV - 1 in corneal scraping and tears of patients and control subjects. Interlukin - 17 (IL - 17A), tumor necrosis factor - alpha (TNF - ?) and interferon - gamma (IFN - ?) were measured in tear samples from patients and control subjects by the ELISA sandwich method. In tear samples; results of RT - PCR showed that 39/52 (75%) of the total patient's infected eyes were positive, 12/16 contralateral eyes were positive (75%). Corneal scraping and tear samples were taken from 16 patients with corneal ulcer, tear samples 14/16 (87.5%) positive result, while the corneal scraping samples were 16/16 (100%) positive, this indicates clearly that the cornea scraping is more appropriate and sensitive sample to detect HSV - 1 than tear. All controls were RT - PCR negative. IFA results of IgA and IgG in tears of infected and contralateral eyes were found to be more than the concentration in tears of control eyes with a highly significant p - value (0.0001). Comparison between the infected and the contralateral eyes indicates that both patients' eyes expressed a significant humoral immune response against HSV - 1. Although there were high significant differences between both the mean value of tears IL - 17A in infected and contralateral eyes with control, while the comparison between patient both eyes gave non - significant difference. The mean of tears TNF - ? and IFN - ? concentration in infected eyes of patients as compared to control was found to be highly significant (p= 0.0001). In this study we compared between patients infected eye with control to evaluate the performance of different markers in tear as HSV - 1specific sIgA, HSV - 1 specific IgG, IL - 17, TNF - ? and IFN - ? by using statistical test called Receiver Operating Characteristic (ROC) curve, the sensitivity for all studied parameters ranged from 80% - 96%.At the same time the comparisons between patient contralateral eyes and controls also revealed a high sensitivity range for these parameters ranged from 81% - 100%.These findings proved the ability of these markers in confirming diagnosis of HSV - 1 keratitis. In conclusion, the high percentage of RT - PCR results for corneal scraping sample indicate that this sample and by using this technique is more appropriate and sensitive to detect HSV - 1 than tear samples and detect the shedding of virus in both eyes of the same patients. Both patient eyes indicated that they were a good source for HSV - 1 diagnosis. In spite of the high sensitivity of all studied markers and their diagnostic ability, TNF - ? showed a higher sensitivity. To assess the immunological status of Herpetic keratitis patients, the studied markers TNF - ?, IFN - ? and IL - 17 were evaluated and showed a highly significant differences which indicated there was an active inflammatory condition combined with HSV - 1 infection and finally it can be concluded that both humoral and cellular branches of immune responses were involved in this disease.

تقييم دور مؤشرات حيوية مختارة لدى مرضى التصلب المتعدد == Assessment Of The Role Of Selected Biomarkers In Multiple Sclerosis Patients

Author name: ميساء نجاح امين
Supervisor name: عبد العظيم ياسين عبود البراك | سرمد عبد الرسول الماشطة
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: التصلب المتعدد (MS) هو من امراض الجهاز العصبي المركزي الاكثر شيوعا الذي يسبب الاعاقه الدائمه لدى الشباب البالغين. بناء على ادلة غير مباشرة قوية، يعتبر مرض التصلب المتعدد اضطراب المناعة الذاتية لعضو محدد ولكن لا يزال هناك الكثير يجب ان يفهم عن كيفيه نشوء ا | Multiple sclerosis (MS) is the most common disease of the central nervous system that causes permanent disability in young adults. Based on strong circumstantial evidence, MS is considered to be an organ - specific autoimmune disorder, but much remains to be understood about the initiation of the disease. It seems unlikely that MS results from a single causative event, but rather the disease develop in a genetically susceptible population as a result of environmental exposures. The present study was planned to determine the serum levels of IL - 17A, IL - 21 and Myelin Oligodendrocyte Glycoprotein (MOG) in patients and control subjects by a sandwich ELISA test using commercially available kits. Vitamin D total was measured in a group of patients and control subjects by cobas e 411 analyzers which is an automated multichannel analyzer for immunological analysis by the use of electrochemiluminescence (ECL) technology.Forty Iraqi MS patients attending the Consultation Clinic for Multiple Sclerosis / Baghdad Teaching Hospital during the period January 2013 - July 2013 were recruited for this study. For the purpose of comparison, 40 control subjects matched by age and gender were also included.The mean serum level of MOG in patient and control (554.85 vs. 315.20pg/ml) and IL - 17A (11.42 vs. 8.33pg/ml) was significantly higher in MS patients as compared to controls, while the mean serum level of IL - 21 (176.164 vs. 75.714pg/ml) was not significantly higher in MS patients as compared to controls. The mean serum level of vitamin D (n=20) (10.38 vs. 13.06ng/ml) was not significantly lower in MS patients as compared to controls. There was a positive correlation between MOG with IL - 17A and between MOG with IL - 21. These results might illustrate the synergism of action between antibody and T cell response in MS patients. A positive correlation was also detected between IL - 17A and IL21 in MS patients.A significant negative correlation was detected between Vitamin D with MOG. A negative correlation was recorded between Vitamin D and IL - 17A in patients. Also negative correlation was recorded between vitamin D and IL - 21 in MS patients. In conclusion, higher level of IL - 17A and MOG in MS serum indicates an important role of this cytokine and MOG as an auto antigen in the pathogenesis of multiple sclerosis and demyelination process, so they could be considered as a good biomarkers for diagnosis and considered as a target for future therapy.

دراسة جزيئية ومناعية لدى عينة من مرضى التدرن الرئوي == Molecular And Immunological Study In A Sample Of Pulmonary Tuberculosis Patients

Author name: مي يحيى عبد الحسين
Supervisor name: عروبة خالد عباس | احمد اسمر منخي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: يعتبر مرض السل الرئوي tuberculosis الذي تسببه بكترياMycobacterium tuberculosis من الامراض المعدية والشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم, ولاهميته اجريت هذه الدراسة لتسليط الضوء اكثر حول التشخيص المناعي للمرض وعلاقته بالتشخيص الجزيئي.تضم | Tuberculosis (TB) is caused by Mycobacterium tuberculosis; which consider as one of the most common, infectious diseases and major causes of morbidity and mortality worldwide. A prospective study was conducted to diagnose disease by immunological methods and its association with molecular diagnosis.The study included immunological diagnosis by Quantiferon - TB Gold In Tube Test, estimation the serum levels of IFN - ?, TNF - ? and IL - 10 in TB patients and control by a sandwich ELISA test using commercially available kits, in addition to molecular diagnosis by using Gene Xpert MTB/RIF assay, conventional bacteriological tests such as smear microscopy by Ziehl - Neelsen stain for sputum samples. Furthermore, estimation of ESR and WBCs.Fifty TB patients attending the National Reference Laboratory of Tuberculosis /Baghdad from the period between April to July 2014 were recruited in this study. For the purpose of comparison, 50 control samples (respiratory disease and healthy) matched by age and gender were also included.All TB patients' samples were positive by smear microscopy. The results revealed that there were significance differences between AFB scoring and G - Xpert results (p Forty five samples of TB patients with significance difference (p?0.01) were positively detected by Quantiferon - TB Gold Test.The mean serum level of QFT among TB patient and control (3.54 IU/ml vs. 0.866 IU/ml and 0.556 IU/ml), IL - 10 (53.02 Pg/ml vs. 22.24 pg/ml and 7.51 pg/ml) and TNF - ? (74.34 Pg/ml vs. 49.12 Pg/ml and 27.81 pg/ ml) were significantly higher in TB patients as compared to controls (P?0.05).In conclusion, high levels of IFN - ?, TNF - ? and IL - 10 in TB patients serum indicate an important role of theses cytokines in the pathogenesis of tuberculosis, so they could be considered as a good biomarkers for diagnosis and considered as a target for future therapy. The present study revealed significant difference in the WBCs and ESR values between TB patients and control (P?0.05)

دراسة خمج فيروس الكبد نمط جي HGV في مرضى الثلاسيميا المصابين وغير المصابين بفيروس الكبد سي (HCV) واهميته السريرية

Author name: مريم صبري ابراهيم
Supervisor name: اروى هادي الحمداني | اشنا جمال فائق
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: المرضى المصابين بفقر دم البحر الابيض المتوسط هم اكثر عرضة للاصابة بالالتهابات الفيروسية المنقولة دمويا. التهاب الكبد الفيروسي نمط جي (HGV)هو فيروس رنوي(RNA) مرتبط بالتهاب الكبد الفيروسي الحاد والمزمن. التهاب الكبد الفيروسي نمط سيHCV) ) والتهاب الكبد الفير | Thalassemia patients are at increased risk of infection with parentally transmitted viral agents. The hepatitis G virus (HGV) is an RNA virus, which is associated with acute or chronic hepatitis. Hepatitis G virus (HGV) and hepatitis C virus (HCV) infections may have a role in complicating the clinical outcome in patients with thalassemia. This study was designed to investigate the prevalence and clinical importance of HGV infection in thalassemia patients with or without hepatitis C virus (HCV) co - infection, furthermore to sequence and analyze phylogentic of HGV clones. One hundred fifty four thalassemia patients (56.5% male, 43.5% female) with a mean age of 22.84±6.06 years were involved in this study that was conducted in the period between Feb. to May, 2014. Anti - HCV antibody was determined by Enzyme Linked Immunosorbent Assay (ELISA) and confirmed by western blot. Then, HCV - RNA was detected in 53.2% of anti - HCV positive thalassemia patients. HGV antibodies were evaluated by ELISA. Also, the HGV viremia was analyzed in patients with thalassemia by reverse transcription polymerase chain reaction (RT - PCR) protocol. Results obtained showed that the anti - E2 - HGV were found in 16 out of 154 (10.4%) thalassemia patients with significant decrease in its prevalence with increasing age, while HGV viremia was diagnosed in 28 out of 154 (18.2%) patients with thalassemia. No association of HGV infection was found with gender, age and frequency of blood transfusion.According to HCV and HGV infection status, thalassemia patients were categorized into four subgroups : subgroup I (HGV infection), subgroup II (HCV infection), subgroup III (co - infection of HCV and HGV), and subgroup IV (thalassemia patients with neither HCV nor HGV infection). Seven point one percent (7.1%) of thalassemia patients were found to be co - infected with HCV and HGV.In all thalassemia subgroups, liver transaminases; alanine transaminase (ALT) and aspartate transaminase (AST), alkaline phosphatase (ALP), and total serum bilirubin (TSB) were determined, in addition to measure serum ferritin levels by VIDAS. HCV infection shows significant increase in ALT level while in HGV is not. Co - infection with HCV and HGV decrease ALT levels when compared with infection with HCV alone, so HGV infection is suggested to have no role in increasing the severity of liver diseases in the thalassemia patients. In HGV infection, there were significant increase in ALP levels and significant decrease in ferritin levels than other subgroups in the study. However, HGV infection shows no significant differences in AST and TSB than other subgroups. The results of genotyping in 12 randomly selected patients showed presence of genotype 2 and genotype 5 with percentage of 91.7% and 8.3% respectively. The diagnosis of prevalence of HGV and HCV in patients with thalassemia in Iraq emphasized the importance of these lymphotropic viral hepatitis infections in pathogenesis and outcome of thalassemia patients.

دور الايبشتاين بار فايرس كعامل محتمل لتقدم سرطان الدم اللمفاوي المزمن == Molecular Characterization Of Metallo ? - Lactamase (MBL) Genes

Author name: لمى عامر ياسر
Supervisor name: عامر رشيد النجار | داود سلمان داود
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Epstein Barr virus (EBV) is ubiquitous virus which infects the majority of the human population and is the causative agent of infectious monocleosis and a variety of B - cell tumors including Hodgkin lymphoma, Burkitt lymphoma, human immunodeficiency virus - associated lymphomas and post - transplant lymphoproliferative disorders , this virus expressed many protein and it is associated with a variety of B - cell tumors. Recently many studies have suggested a causal relationship between EBV and chronic lymphocytic leukemia (CLL). A prospective study of thirty samples of formalin - fixed, paraffin - embedded tissue of bone marrow aspirates samples and blood from newly diagnosis BCLL of 30 patients and our control included : 1) 20 bone marrow of patients who they have hematological disease other than leukemia as control. 2) Blood serum from healthy controls to study the level of IL - 10 and IL - 8. The present study conducted at Baghdad Teaching hospitals and the National Center of Hematology from September 2013 to Jun 2014. In this study we investigated the association between EBV and CLL. The detection of EBV encoded RNAs (EBER1,EBER2) by in situ hybridization and also latent membrane protein - 1 (LMP - 1) and EBV nuclear antigen - 2(EBNA2) by immunohistochemistry and detection for the level of Interleukin n (8 and 10) in the serum of CLL patients by ELISA. In situ hybridization study revealed that all the controls were negative for EBERs and 46.7% patients were found to be EBERs positive. There was a correlation between positive EBERs and tumors stage and also EBERs and IL10 and with LMP - 1 and EBNA - 2. Immuno - histochemical method was used to demonstrate the rate of (LMP - 1 and EBNA - 2) in CLL patients where a positive results of EBNA2 and LMP1were found in (43.3 % and 56.6s %), respectively in CLL patient group. All controls were negative with an exception of two patients were LMP1 positive. Enzyme linked immunosorbent assay test (ELISA) was used to detect the level of IL - 8 and IL - 10 in serum of newly diagnosed of CLL patients. Interleukin 8 was significantly higher in CLL cases group (33.1 pg/ml) compared to control group.On the other hand It was found that high increase in IL - 10 level ,in CLL patients when compared with controls. Conclusion : It can be concluded that there is a significant relationship between EBV and Chronic lymphocytic leukemia, mainly EBERs, also the association of significant increase in IL - 10 and IL - 8 with CLL.

مؤشرات جزيئية حول مقاومة الزوائف الزنجارية المرتبطة بخمج المجاري البولية للمضادات الحيوية == Molecular Markers Of Antibiotics Resistance Of Pseudomonas Aeruginosa Associated With Urinary Tract Infections

Author name: كريم عليوي حمادي سليمان
Supervisor name: عامر رشيد النجار
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: الزوائف الزنجارية : وهي بكتريا سالبة لصيغة الكرام والمنتشرة بشكل واسع في البيئة مثل التربة والمياه. وهذه البكتريا تتسبب في كثير من الالتهابات مثل التهاب المجاري البولية والتهاب الحروق وهي بكتريا انتهازية. وتشكل تهديد لحياة المصابين بالمجاري البولية وخاص | Pseudomonas aeruginosa is a Gram |( - ve) bacteria, widely presents in the environment such as soil, water, its incubation period (1 - 3) days, generation time 1 - 2 hrs. This bacteria causes many infections such as (UTI), and burn infections. It is an opportunistic pathogen, life threating for immune compromised patients such as diabetic patients. Pseudomonas aeruginosa is a nosocomial pathogen, and it is ranking the second among Gram ( - ve) hospitals acquiring pathogen. Its antibiotic resistance increased due to acquiring many antibiotics resistance genes. The spread of these genes among bacteria is via plasmids. Plasmid profiling of Ps. aeruginosa isolates by gel electrophoresis is a best technique for detection of plasmid content. Through this study, which was carried out in two main teaching hospitals, the diabetic center and private labs in Baghdad, during the period from January to October 2014. A total of (620) urine specimens were collected during (9) months. Urine specimens from inpatients were (420 / 620, 67.75%) and from out patients were (200 / 620, 32.25%). Urine specimens from Dialysis unit were (280 / 620, 45.1%) and from burn units were (140 / 620, 22.5%). The male represented (n = 334, 52.38%) while female were (n = 286, 46.20%). The positive cultures of urine samples obtained from hospitalized patients were (72 / 420, 17.1%) while for urine samples obtained from out patients (n = 40 / 200, 20%) were positive culture. A total of (112) positive culture out of (620) collected with (18.06%) percentage. A total of (30) bacterial isolates were tested by using disc diffusion method for susceptibility test forward to (15) antibiotics. Ps. aeruginosa isolates showed highest resistance to gentamicin (87.5%) while the lowest resistant toward meropenem, and imipenem with resistance percentage (16.7% and 8.0%) respectively.The occurrence of multi - drug resistance in (30) selected isolates were grouped as follows to (MDR) for those isolates which showed resistance to at least (3) antibiotics which was found to be (5 / 30, 6.6%). While (XDR) referred to those isolates which showed resistance to (4 - 5) antibiotics with (19 / 30, 63.3%). In related to (PDR) which referred to isolates that showed resistance to more than 5 with (6 / 30, 20%). The extracted plasmid DNA were resolved by gel electrophoresis for detection of plasmid content for (20) isolates including (10) isolates from inpatients, and (10) isolates from out patients. The selection of these isolates were grouped according to their antibiotic resistance pattern and the number of plasmids that they carried. The results showed that out of (20) there were (12 / 20, 60%) had no plasmid. While the remaining (8 / 20, 40%) had plasmids with different sizes and numbers. By using PCR technique the most common kinds of genes such as bla CTX - M, bla OXA, which encoded extended spectrum ? - lactamase (ESBLS) and those for metallo ? - lactamase such as bla IMP, were investigated with specific primers. Five isolates were selected depending on the numbers of plasmids which they carried and their resistance to antibiotics.

دراسة مؤشرات مناعية وبايوكيميائية مختارة لمرضى السكري النوع الثاني المصحوب وغير المصحوب باعتلال الكلى ذات الادرار قليل الزلال == Study Of Selected Immunological And Biochemical Markers In Type 2 Diabetes With And Without Microalbuminuric Nephropathy

Author name: علي ناصر محمد علي
Supervisor name: عبد العظيم ياسين عبود البراك | حازم عبد الرزاق عبد الوهاب
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Diabetic Nephropathy (DN) is a common complication of diabetic diseases occuring all over the world including Iraq. This type of microvascular complication of diabetes mellitus represents the most common reason of end stage renal disease (ESRD) in the world which considers the main reason for hemodialysis, kidney transplantation and death in developed countries. For this reason the assessment of some biomarkers as an early predicator before onset of microalbuminuria stage of diabetic nephropathy and the correlations between these biomarkers with microalbuminuria were carried out.This study was achieved at AL - Yarmouk Teaching Hospital and the Diabetes National Center /AL - Mustansiriyah University/Baghdad from January 2013 to September 2014. The study involved ninety individuals, twenty volunteers selected from the local community; apparently healthy (group I), 40 type 2 diabetics with normal urinary albumin to creatinine ratio (UACR) (<30 mg/g) (group II), and 30 type 2 diabetic patients with abnormal UACR (? 30 - 300 mg/g) (group III). Mean of diabetes duration (D.D.) in group II is about 4 years and in group III is about 8 years. The mean age for group I, II and III were 53, 55 and 58 years respectively. The concentration was measured of each microalbuminuria by turbidmeteric method, estimated glomerular filtration rate (eGFR) by using chronic kidney diseases - epidemiology equation, glycated heamoglobin (HbA1c) by using high performance liquid chromatography (HPLC), both fasting blood sugar (FBS) and creatinine (serum and urine) by using colorimeteric method, body weight by using body mass index (BMI) and all of IL - 18, IL - 12 IL - 4, IFN - ? and urinary vitamin binding protein (VDBP) by using ELISA method in three groups.The present study showed that there is inverse correlation between UACR and eGFR. The cause of this inverse correlation is that the decline in renal function of diabetics can be predicted accurately by using both UACR to show the increase in microalbuminuria while eGFR show a measure of the decrease in the ability of kidney for filtration.The difference of FBS mean between group I and II and group I and III was significant (P= 0.000 for both). The P - value between II and III groups was significant (P= 0.024). The difference of HbA1c mean between group I and II, group I and III and group II and III was significant (P=0. 000 for all). These results were expected as high HbA1c level is in consequence of high FBS. The difference of BMI mean between group I and II, group I and III and group II and III was not significant (P= 0.870, 0.885 and 0.968 respectively). Because the BMI levels were approximately constant in three groups. The difference of D.D. between group II and III was significant (P= 0.000).There was no significant correlation in group II between urinary albumin to creatinine ratio with each of diabetic duration, glycated haemoglobin, fasting blood sugar and body mass index (r=0.219 with P=0.244, r=0.039 with P=0.840, r=0.080 with P=0.673 and r= - 0.126 with P= 0.506 respectively); but there was positively significant correlation between urinary albumin to creatinine ratio with each diabetic duration, HbA1c and fasting blood sugar in group III (r=0.298 with P=0.043, r=0.869 with P=0.000 and r=0.518 with P=0.003 respectively) which may be due to the chronic nature of the disease in this group that require these risk factors to initiation; however, the correlation was non significant between UACR and BMI (r= - 0.127 with P=0.228) in group III.The difference of S.Cr. among I and II groups, I and III groups and II and III groups were not significant (P= 0.998, P= 0.331 and P= 0.145 respectively).The correlation was not significant in group II between serum creatinine with each of diabetic duration, glycated haemoglobin, fasting blood sugar and body mass index (r= - 0.140 with P=0.462, r= - 0.124 with P=0.515, r=0.168 with P=0.374 and r=0.007 with P= 0.969 respectively). Also, there was no significant correlation in group III between S. Cr. with each D.D., HbA1c, FBS and BMI (r=0.187 with P=0.077, r=0.109 with P=0.220 and r=0.175 with P=0.124 respectively). The reason of these results backs to that S. Cr. level is not increased as the podocytes remain intact in the diabetics with and without microalbuminuria.The difference of eGFR between group I and II was not significant (P= 0.303). The difference between group I and III and group II and III was significant (P= 0.001 and 0.010 respectively). The correlation was not significant in group II between eGFR with each of diabetic duration, glycated haemoglobin, fasting blood sugar and body mass index (r=0.121 with P=0.524, r= - 0.180 with P=0.341, r= - 0.310 with P=0.096 and r=0.021 with P= 0.911 respectively). Also, there was no a significant correlation in group III between eGFR with each diabetic duration, HbA1c, FBS and BMI (r= - 0.179 with P=0.07, r= - 0.188 with P=0.061, r= - 0.123 with P=0.388 and r= - 0.112 with P=0.557 respectively). The reason of these results back to that eGFR level is at the normal range in group II and slightly beneath the normal range in group III in consequence of the podocytes remain intact in the diabetics with and without microalbuminuria.The difference of urinary VDBP level between group I and II, group I and III and group II and III was significant (P= 0.000 for all). The correlation between urinary albumin to creatinine ratio levels and VDBP level (r=0.963) with P - value 0.000 which was positively significant in group III. The correlationbetween eGFR and VDBP was - 0.524 with P - value 0.003 which is inversely significant in group III. The reason of this inverse correlation may be similar to the reason of inverse correlation between eGFR and UACR, as the cubilin - megalin receptors are common receptors for albumin and vitamin D binding protein. Otherwise, the correlation between urinary VDBP and eGFR in the group I was a weak negative with non significant P - value (r= - 0.188, P=0. 428). The reason of this non significant correlation is that the cubilin - megalin receptors are not damaged by inflammatory process to elevate VDBP in urine; also the podocytes in glomeruli are intact from the damage by inflammatory process, so the eGFR was at the normal range in the control group.The correlation was a positive between HbA1c and VDBP levels in group II and group III (r=0.579, P=0. 001 and r=0.686, P=0.000 respectively). This positive correlation was explained on the basis that deterioration of sugar level control lead to increase the proinflammatory cytokines that damage cubilin - megalin receptors then VDBP increase in urine.The difference of serum IL - 18 level between group I and II, group I and III and group II and III was significant (P=0.000 for all).The correlation between urinary albumin to creatinine ratio and IL - 18 was 0.983 with P - value 0.000 which was positively significant in group III. This may back to damage the cubilin - megalin receptors by IL - 18 action that lead to increase of UACR in urine. In other words, the correlation between serum creatinine and serum IL - 18 in group III was not significant (r=0.041 with P= 0.830). This may back to that podocytes remain intact in this early stage of diabetic nephropathy. There is a significant positive correlation between serum IL - 18 levels and HbA1c levels in group II and group III (r=0.641, P=0.000 and r=0.721, P=0.000 respectively). These two positive correlations support the suggestion of choosing serum IL - 18 as an excellent biomarker for avoidance an early stage of the disease.The difference of IFN - ? levels between group I and group II, between group I and group III and between group II and group III were not significant as follows : (P=0.640, P=0.292 and P=0.522 respectively). The correlation between UACR and IFN - ? (r=0.047) with P - value 0.830 which was not significant in group III which means that IFN - ? is not a good biomarker for prediction of the microalbuminuria as an early stage of DN.The difference of IL - 12 level between group I and group II, between group I and group III and between group II and group III were not significant (0.884, 0.431 and 0.439) respectively. The correlation between UACR and IL - 12 (r=0.190) with P - value 0.314 which was not significant in group III.The difference of IL - 4 levels between group I and group II, between group I and group III and between group II and group III were not significant (0.943, 0.704 and 0.648 respectively). The correlation between UACR and IL - 4 (r=0.169) with P - value 0.371 which was not significant in group III.These results of IFN - ?, IL - 12 and IL - 4 might lead to conclude that both cytokines can’t be selected as a biomarker for an early detection of DN. Finally from the all presented data it can be concluded that IL - 18 and VDBP are considered more sensitive and more efficient than a classic diagnostic method (UACR and eGFR) for avoidance and detection the early stage of DN.

مميزات التحويرات المناعية للريسفراترول المستخلص من قشور العنب الاحمر على التهاب الكبد المحدث باستخدام ديكالاكتوزامين والذيفان المعوي نوع ب للمكورات العنقودية == Immunmodulatory Properties Of Resveratrol Extracted From Skin Of Red Grape On Acute Liver Injury Induced By D - Galactoseamine And Staphylococcus Enterotoxin B

Author name: صباح زيارة كاظم المالكي
Supervisor name: عبد العظيم ياسين عبود البراك | متزي نكاركاتي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: تعتبر اصابة الكبد الحاد احد الامراض ذو الاعراض السريرية التي تتصف بارتشاح الخلايا اللمفية الى الكبد وتجمع السوائل وارتفاع انزايمات الكبد مثل ناقل امين الاسبارتات Aspartate aminotransferase(AST) في مصل الدم. هنالك الكثير من الاسباب التي تؤدي الى حدوث ضرر | Acute Liver Injury is a vital clinical syndrome characterized by infiltration of lymphocytes in the liver, fluid accumulation, and elevation of liver enzymes such as Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum. There are many causes of liver injury, but in this particular study Staphylococcal Enterotoxin B (SEB) was used to induce acute liver injury in mice, it is previously known that (SEB) it act as superantigen that bind with T cell receptor variable region beta chain (VB8), and MHC II of Antigen Presenting Cells (APCs), which leads to activation of T - cells, and huge secretion of pro - inflammatory cytokines, all of these events cause an acute liver injury lead to liver failure, and death. In the last few decades the importance of some natural products appeared, in which these botanicals have an anti - inflammatory properties. Resveratrol is one of these botanicals with an anti - inflammatory effects, which was used as treatment in this study.This study was particularly intended to study the influence of Resveratrol on acute liver injury induced by Staphylococcus Enterotoxin B, lead to liver failure which includes estimation of aspartate aminotransferase levels (AST), histopathological analysis, immune cell counts of liver and spleen, in addition, study whether resveratrol has the ability to cause immunological changes on different immune cells that lead to suppress acute liver injury by using flowcytometry technique,these cells are inflammatory cells carrying CD3+ T cells, CD4+ T cells, CD8+ T cells, NK1.1+ T cells, CD44+ T cells, and suppressive cells such as Myeloid Derived Suppressor Cells (MDSCs), T regulatory cell (FOXP3), as anit - inflammatory markers. Furthermore evaluate the pro - inflammatory, and anti - inflammatory cytokines by using Bio - plex. Also to determine the molecular mechanism in which resveratrol can lead to induce changes in microRNAs (miRNAs) by microarray analysis. Finally validation (miRNAs) resulting from microarray analysis and genes of interest associated with these miRNAs by Real time polymearase chain reaction (RT - PCR).After sixteen hours blood samples were collected to determine AST levels in differents groups using Nanodrop, (Fisher - USA), after sixty hours animals sacrificing organs (liver, spleen) were collected afterward liver samples in 10% formalin were sent for histopathological study, liver immune cells were isolated for counts and molecular study (microarray analysis and quantitive (RT - PCR) total RNA was isolated from liver immune cells for microarray analysis then preparation of complementary Deoxyribonucleic acid (cDNA) by Reverse transcriptase PCR. Validation of the microarray analysis data was achieved by Real time PCR. Spleen immune cells were collected afterward for flowcytometric analysis of CD3+, CD4+, CD8+, NK1.1+, CD44+ T cells, MDSCs, and T regulatory cells using cell surface staining and intra cellular staining.This study showed that Resveratrol oppose the effect of SEB induced acute liver injury through decrease AST concentrations (P< 0.0001). Also Resveratrol 50mg/Kg body weight led to cause reduction in the size of the spleen, compared to SEB+ Vehicle group. Histopathology study reported that resveratrol led to decrease of hepatic necrosis up to 10% in comparison to vehicle group with 60% of hepatic necrosis and decrease in the inflammatory response represented by immune cells as in liver immune cell counts which showed a significant decrease (P< 0.014) after 50mg/ Kg body weight of Resveratrol treatment. Spleen immune cell counts result reported a significant decrease (P<0.013) in the total cells when Resveratrol was used as treatment compared to disease group. Regarding to Flowcytometric analysis, it was found that Resveratrol significantly decrease the absolute count of CD3+, CD4+, CD8+, NK1.1+, CD44+, Foxp3+ T cells in C57BL/6 mice (P< 0.0019, P< 0.001, P< 0.009, P< 0.002, P< 0.03, and P< 0.03 respectively). While MDSCs result reported significant increase with P< 0.01 in absolute cell counts after Resveratrol treatment. Depending on the Bio - plex data showed significant decrease in serum concentrations of inflammatory cytokines IL - 1?, IL - 1?, IL - 3, IL - 5, IL - 12p(40), IL - 12p(70), IL - 13, IFN?, MIP - 1?, MIP - 1?, KC and Rantes cytokines with significant P values (0.009, 0.004, 0.034, 0.004, 0.0009, 0.01, 0.0007, 0.009, 0.0037, 0.0001, 0.0035, 0.006 respectively), while anti - inflammatory cytokines IL - 6, IL - 10 and G - CSF c showed a significant increase in the serum concentration after Resveratrol treatment with a P values of, 0.012, 0.02 and 0.001 respectively. Microarray analysis results recognized 451 miRNAs with P< 0.05, according to fold change expression, 78 miRNAs have fold change greater than - 1.5 fold of expression in Resveratrol treatment group (downregulated), while 87 miRNAs have fold change greater than 1.5 fold of expression (upregulated). According to the ingenuity tools predicted target gene for some of these miRNAs by using miRNA.org database, the database showed that miR - 130a - 3p with - 2.28 fold expression have direct relation of binding with CSF1 gene, Real time PCR results confirmed the results obtained from microarray analysis, the result of miR - 130a - 3p showed a significant decrease of relative fold expression with P< 0.013, and Colony Stimulating Factor - 1 CSF1 (M - CSF) relative expression increased significantly after resveratrol treatment with P< 0.0001. From all of previous data, it can be concluded that Resveratrol can counteract acute liver injury induced by SEB, by decrease AST concentration. Resveratrol acts as an anti - inflammatory compound due to decrease of immune cell numbers, decrease of inflammatory markers, and increase of anti - inflammatory markers. miR - 130a - 3p with inflammatory properties downregulated after resveratrol treatment. Finally Resveratrol treatment increased relative fold expression of CSF1(M - CSF); gene which play a role in the MDSCs proliferation

دراسة معظم السمات المناعية وتحديد البكتريا الابرز في مرض التهاب السحايا باستعمال تقنية البي سي ار == Study Some Immunological Aspects And Determination Of Most Prominent Bacteria In Meningitis Patients By Using Real Time Pcr

Author name: شيماء عبد الرضا حميد
Supervisor name: زينب فاضل عاشور | دريد قاسم الشريف
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Meningitis, which is inflammation of the membranes that surround the brain and spinal cord and can occur at any age, it is caused by infectious microorganisms include (Bacteria, Viruses, Fungi, and Parasites) and non infectious include (Drugs, Carcinoma and Inflammatory disorder), also requires early treatment with aggressive use of potent antimicrobials; otherwise the outcome almost ends fatally.The predominant causes of bacterial meningitis are streptococcus pneumoniae, Haemophilus influenzae type b (Hib) and Neisseria meningitides.This prospective study includes 40 cases of meningitis which have been admitted to the Baghdad Teaching Hospital and Child Welfare Teaching Hospital during the period from May to November/ 2014.The detection of meningitis causative agent were specified by using the standard biochemical and microbiological methods such as : Determination of protein and sugar in Cerebrospinal fluid, Differential and cell count, growth on Culture media and morphological appearance on Gram's stain.Confirmative tests were also done for identification of meningitis causative agent; these tests included Procalcitonin, IL - 6, CRP, D. Dimer. These confirmative tests have been crowned by PCR technique, by employing the detection of the bacterial gene.The DNA of bacteria was extracted from CSF of meningitis patients and detection the type of bacteria gene by using multiplex PCR technique that using primers to amplify each ply, bexA and ctrA gene.Out of 40 samples, 28 (70%) were identified as bacterial meningitis and 12 (30%) as viral meningitis, the result of PCR reaction revealed unmistakably that 20 (71.4%) of 28 were detected the bacteria (S. pneumonia, H. influenzaetype b and N. meningitides) as a causative agent, while there were no detectable infectious agents in the (40) control group included in this study.Viral meningitis patients showed the number of lymphocytes (31.92 cell/ mm3) and neutrophils (1.00 cell/ mm3), while the highest number of lymphocytes and neutrophils in bacterial meningitis caused by S. pneumoniae (77.08 cell/ mm3) , (28.86 cell/ mm3) respectively, so that there was significant difference between cells (lymphocyte and neutrophil) with study group at P value = 0.010, P value = 0.013 respectively. In microbiological examination 22.5% of patients group (40) showed positive result of culture and 7.5% showed positive result of gram stain.In biochemical tests, the detection of Cerebrospinal fluid's protein and glucose level was done. The level of protein concentrations in bacterial meningitis with S. pneumoniae (103.08 mg/l), H. influenzae (65.14 mg/l) and in other bacteria was (124.44 mg/l) which is higher than the protein concentration in viral meningitis (42.25 mg/l), and these results showed highly significant difference at (P=0.010). Results of glucose's level determination showed that there were no significant difference among the studied groups (P =0.2). Results of glucose's level determination showed the presence of non significant difference among the studied groups, (60.92 mg/dl), (43, 57 mg/ dl), (51.00 mg/dl), (41.50 mg/dl) consecutively appeared in bacterial, viral.Interleukin - 6 and Procalcitonin levels were diagnosed by Enzyme Linked Immunosorbent Assay, while the plasma D. Dimer and erum C - Reactive protein was diagnosed by NaycoCard technique.The current results showed the presence of high concentration of IL - 6 among meningitis patients, high concentration of serum IL - 6 appeared in bacterial and viral meningitis (28.68 pg/ml) when compared with control group (2.194 pg/ml) (P =0.001), while CSF IL - 6 show high concentration in bothbacterial and viral meningitis but with no significant difference (579.24 pg/ml) (P =0.0001). The data of this study showed, there was correlation between IL - 6 levels and neutrophil in meningitis patients. Interleukin - 6 had significant correlation with neutrophil (P =0.009). While no significant correlation appeared between IL - 6 and Lymphocyte.Procalcitonin results showed high concentration in bacterial meningitis group compared with viral and control group with a highly significant differences (P =0.0001).The results of CRP shown no significant differences between bacterial and viral group, while the levels of D. Dimer appear significant differences in both groups when compared with control group.Current study indicated that PCR, Procalcitonin and D. Dimer played an important role in diagnosis of meningitis disease.

العلاقة بين مرض السكري المناعي الذاتي الكامن النمط الثاني للبالغين مع الخمج بالملوية البوابية == Association Between Latent Autoimmune Diabetes Of Adults In Type Ii Dm With Helicobacter Pylori Infection

Author name: سيف علي مكلف
Supervisor name: عروبة خالد عباس | مدين علي حسين
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Latent Autoimmune Diabetes in Adults (LADA) is a form of diabetes which is less recognized and underdiagnosed type of diabetes which appears to have characteristics of both type 1 (autoimmune in nature) and type 2 diabetes (adult age at onset).Helicobacter pylori (H. pylori) infection has a worldwide distribution. The bacteria colonize more than half of the world population and consider the main cause of gastritis, peptic ulceration, gastric adenocarcinoma and gastric lymphoma. The host immune response is unable to clear the infection and may actually contribute to the associated pathogenesis, also the bacterial virulent factors had certain role in the pathogenicity of infection as it was stated by limited studies that the Cytotoxin associated gene A (CagA) protein and Vacuolating cytotoxin gene A (VacA) protein positive strains are possibly associated with severe infections.The present study was designed to find out the percent of LADA patients among group of type II Iraqi diabetic patients and the relation between LADA patients and H. pylori infection.The study was carried out on 350 type II patients attending the National Diabetes Center/ Al - Mustansirrhea University/ Baghdad Al - Yarmouk during the period May to October 2014 were recruited for this study. Diagnostic markers for LADA, namely, C - peptide levels by Radioimmunoassay and GAD autoantibodies by ELISA test were determined and other Various parameters such as, HbA1c levels, Anti - H.pylori IgG, Anti - CagA protein and Anti - VacA protein by ELISA test were evaluated and correlated with type 2 diabetes and 40 control, matched by age and gender were also included.57 patients of 350 of type II have low C - peptide level, from which, 40 patients have Anti - GAD65 positive and 17 patients have Anti - GAD65 negative.The percentage of LADA patients among type II diabetes was 11.4%.The mean serum levels of C - peptide of LADA patients, controls and type II diabetes (0.30 vs. 1.94 and 3.15ng/ml) were significantly lower in LADA patients as compared to controls and type II patients (p?0.05).Also the mean blood concentrations of HbA1c of LADA patients, type II and controls (8.5 vs. 7.5 and 5.2 %) were significantly higher in LADA patients as compared to type II patients and controls (p?0.05).The Higher frequency of anti - H. pylori IgG was in LADA patients when compared with patient's controls and healthy controls (59.6%, 21.3% and 19.1%) and the p - value (?0.05).In conclusion, Cytotoxic associated gene A protein (Cag A) protein is more frequency with H. pylori positive patients 36/47 (76.6%) indicate an important role of this virulence factor in the pathogenesis of H. pylori, so they could be considered as a good biomarker for discern the risk of developing serious gastroduodenal disease in the host, than Vacuolating cytotoxin A Protein (Vac A) which is less frequency than Cag A in H. pylori positive patients 16/47 (34%).

دور الانترلوكين RA - 1 والسكليروستين والخلية التائية المستجيبة CD4 في مصل مرضى التهاب المفاصل الرثوي == Role Of Serum IL - 1RA, Sclerostin And Effector T - Cell (CD4) In Rheumatoid Arthritis Patients

Author name: سنن ثائر عبد الوهاب
Supervisor name: حيدر صباح كاظم | علاء الدين مظفر زبير
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: التهاب المفاصل الرثوي هو احد امراض المناعة الذاتية الاكثر شيوعا والذي يتميز بالتهاب المفاصل المزمن والذي يؤدي فيما بعد الى تدمير المفاصل.لا يزال مرض التهاب المفاصل الرثوي يعرف على انه سمة من التوسع في الغشاء الزليلي وارتشاح الخلايا الالتهابية اضافة الى | Rheumatoid arthritis (RA) is one of the most common systemic autoimmune diseases characterized by chronic joint inflammation and subsequent joint destruction. It is well known that RA is characterized by the expansion of the synovium and infiltration of the inflammatory cells coupled with destruction of adjacent articular cartilage and bone. This is strongly dependent on CD4 T cell. CD4 Cells stimulate monocytes, macrophages, synovial fibroblasts, and other cells to produce cytokines such as tumor necrosis factor - ? (TNF - ?), interleukin - 1 (IL - 1), IL - 6, IL - 15, IL - 17 and metalloproteinases that produce tissue damage. TNF is a major inflammatory cytokine contributing to the pathogenesis of RA, which provides rational for development of anti - TNF biological agents in the treatment of RA.Wnt pathway (a complex protein network) important in control of the bone formation through the regulation of osteoblast activity, and sclerostin is an important in the regulator of the Wnt pathway by blocking Wnt binding to its receptor and thereby inhibiting bone formation. Blockage of Wnt antagonists such as sclerostin will trigger repair or even healing of bone erosion.Recently have shown that IL - 1Ra has been given therapeutically in several experimental models of arthritis with weak effect in RA. This study planned to evaluate the frequencies of CD4+CD25+ Treg cells in Iraqi patients treated with RA under treatment with biological therapy (Etanercept). The level of serum sclerostin, serum level of IL - 1RA, and investigate the correlation between serum sclerostin and Treg expression. Correlate the level of serum sclerostin and Treg expression with disease activity by (CDAI or DAS28).This study was performed during the period from January 2016 to May 2016.The patients were attending the out patients' Clinic in Medical City/Baghdad Teaching hospital/Rheumatology Unit and the laboratory Enzyme Linked Immunosorbent Assay tests were done in Bio - technology center at the University of Al Nahrain, flow cytometery analysis was performed in the private laboratory (Al Rawabi Laboratory) at Yarmouk/Baghdad. Thirty patients and 30 apparently healthy control individuals were included in this study, Patients under treatment of etanercept.The diagnosis of RA patients has performed under the rheumatologist consultant at the consultation clinic of Baghdad Teaching Hospital of the medical city. Blood samples had taken from RA patients to measure Erythrocyte Sedimentation Rate, Complete Blood Picture, Rheumatoid Factor and High - Density Lipoprotein. Also, analyze the serum levels of Anti - Citrullinated Protein Antibodies and estimate the levels of sclerostin and IL - 1RAin patients and healthy individuals using Enzyme Linked Immunosorbent Assay test kits. CD4+CD25+ Treg cells had achieved by flow cytometery analysis.The results showed the reduction in Treg cells in patients (0.00264 ± 0.002) % under biological therapy than control group (0.026 ± 0.012) % with significant difference (p< 0.05). Also body mass index showed significant difference between patients (32.10 ± 5.81) kg/m2 and control group (28.84 ± 4.67) kg/m2 (p< 0.05). The results showed that sclerostin level higher in healthy (1.072 ± 0.59) ng/ml than patients (0.801 ± 0.28) ng/ml which showed significant difference (p<0.05). Furthermore IL - 1RA serum level was higher in healthy (0.979 ± 0.34) ng/L than patients (0.633 ± 0.35) ng/L with significant difference (p<0.05). Anti - Cyclic Citrullinated Peptide results showed non - significant difference between patients and healthy control. Treg cells is reduction in the patients with RA. Sclerostin and IL - 1RA levels are low in patients treated with etanercept.

دراسة جزيئية للاصابات بفايروس الحليمي البشري وفايروس سرطان الغدد اللبنية البشري في المريضات المجراة لهن عملية استئصال الرحم الكامل == Molecular Study Of Human Papilloma Virus And Human Mammary Tumor Virus Infections Of Patients With Total Hysterectomy

Author name: سري ظافر داود العزيز
Supervisor name: سعد حسن محمد علي | بسام شهاب احمد
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Theoretically , the origin & mode of in - utero transmission of high risk Human PapillomaVirus (HR - HPV) types could be acquired hematogenously, by semen at fertilization or as asending infection in the genital tract. Viral Oncogenes E6 and E7 have ability to altered the Cyclin D1,p21 and p27 protein levels. Worldwide, Human Mammary Tumor Virus(HMTV) infection been described in hormonal responsive tissues.This research work is to study viruses in relaition to endometrial carcinoma in Iraqi patients.Materials and MethodsA retrospective study envolved seventy(70) cases (158 samples) of endometrial and cervical tissues collected from teaching laboratories in Medical City, Al - Yarmok Teaching Hospital and private laboratories related to the period from 2012 to 2014. Sections were made and subjected to chromogenic in - situ hybridization technique to detect the presence of High risk (HR) HPV 16/18 DNA and HMTV integrated DNA (provirus) and immunohistochemical technique to detect the presence of Cyclin D1,P21and P27.Results : The most commonly affected age group was ? 60 year 60.0% in malignant endometrial tumors, 40 - 49 years 36.0% in non - malignant endometrial tumors. Adenocarcinoma was the only histopathological type among endometrial cancer (100%) and the majority have well differentiated grade (90.0% ),and have (70%) T1b/IB stage.Hight risk HPV16/18 expression was detected 33.3% of malignant endometrial tumors, 20% in non - malignant endometrial tumors, andSUMMARY26.7% in control group. The higher rates of expression of HPV in cervical lesions was 40%.Most HPV16/18 infections revealed in both sites (cervix and endomertium) have Punctate DNA pattern.Cyclin D1 expression was mostly in 43.3% of malignant endometrial tumors and 33.3% was in cervical lesions. The significant correlation between Cyclin D1 expression and HR - HPV16/18 infection was reported only in malignant endometrial tumors groups. The highest P21 expression was 40.0% in malignant endometrial tumors and 44.0% in cervical lesions that reveals a significant association between p21 expression and HR - HPV16/18 infection. The highest rates of P27 expression were noticed among non - malignant endometrial tumors 24.0% and 32.0% in cervical lesions.While significant association of P27 expression with HR - HPV16/18 infection was only found in the cervical lesions among malignant endometrial tumors group.The distribution of integrated HMTV genome detected by Chromogenic in - situ hybridaztion (CISH) in the endometrial tissues was 16.7% in malignant endometrial tumor , 8% in non - malignant endometrial tumors, and 13.3% in the control tissues group while in the cervical lesions HMTV was detected in 13.3%. Significant correlations were found between HMTV infection and Cyclin D1 expression in the cervical lesions among malignant endometrial tumors group and p21 expression in the malignant endometrial tumors.ConclusionsHR - HPV16/18 may be associated with initiation of endometrial carcinogenesis events as well as play a role in the progression of such malignant tumors in addition ,these viruses could ascendingly be transmitted from lower part(cervix) to the upper part of the genital tractSUMMARY(endometrium) in some way or other.Cyclin D1 ,P21,and p27 could have sharing in early events of tumorgenesis in endometrial carcinoma and a significant correlations of Cyclin D1 ,p21& p27 expressions with HPV infection was observed among study group.Althought a relating low rates of HMTV infection were detected among different endometrial as well as cervical lesion in this Iraqi study,it might mark or shade alight in the way for further researches in this field to exposure more aspects of this newly studied virus among other hormonal dependent malignancy

تقييم sFas ligand, sFas وcaspase - 3 في المرضى المصابين بمرض ابيضاض الدم النقياني المزمن == Assessment Of Soluble Fas, Soluble Fas Ligand And Caspase - 3 In Patients With Chronic Myeloid Leukemia

Author name: سحر راضي ياسر
Supervisor name: ميسون علي سليم | بسام فرنسيس متي
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: ابيضاض الدم النقياني المزمن هو اضطراب التكاثر النقي النسيلي للخلايا الجذعية ويتميز بواسطة فلادلفيا كروموسوم وهو انتقال متبادل بين كروموسوم 9 و22. الجين المسرطن المتحد الجديد المتولد على كروموسوم 22 كنتيجة للانتقال يسمى BCR - ABL gene. في اغلبية المرضى, هذ | Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of the haemopoietic stem cell, defined by the Philadelphia chromosome (Ph) - reciprocal translocation between chromosomes 9 and 22. The novel fusion oncogenes generated on chromosome 22 as a result of this translocation are called BCR - ABL gene. In the majority of patients, this oncogene transcribes a 210 - kDa constitutively active protein tyrosine kinase, often referred to as p210BCR - ABL, which is necessary for the transformation of the disease. The introduction of tyrosine kinase inhibitor (TKI) - imatinib mesylate (IM) - marked a major advance in CML treatment in terms of efficacy and tolerability and has now become the first line of therapy. In CML, besides genetic change during which a normal cell is transformed into a malignant one, evasion of apoptosis (programm cell death PCD) is one of the essential changes in a cell that cause malignant transformation. So ‘Evading apoptosis’ has been recognized as one of the six hallmarks of cancers, as reduced apoptosis or its resistance plays a vital role in carcinogenesis. Soluble receptors (sFas) that act as decoys, binding FasL and preventing association with transmembrane Fas. So production of sFas in tumor patients may be a key mechanism to inhibit Fas - mediated apoptosis. Soluble FasL competes with the membrane - bound counterpart; however, it can act even as an antagonist preventing apoptosis induction by the membrane integrated form of the ligand. Recently, caspase - 3 has been demonstrated to play an important role in determining the cellular sensitivity to diverse apoptotic stimuli, also it is involved in a number of non - apoptotic events. So the aims of the study were to estimate the level of serum sFas, sFas ligand & caspase - 3 in patients with chronic myeloid leukemia at time of diagnosis (newly diagnosed), then after receiving imatinib myselate treatment with optimal response and compare them with healthy control. It was a prospective study, included 56 Iraqi CML patients (25 were males and 31 were females with Age ranged from 15 - 78 years) attending to Baghdad teaching hospital/hematology department between November 2012 up to June 2013, were categorized by fluorescence in situ hybridization analysis (FISH) for Ph chromosome into two stages, newly diagnosed CML patients (FISH Ph cells result ? 90% ) not received any treatment and optimally treated (complete cytogenetic response (CCgR) = FISH Ph cells result < 1% or by major molecular response (MMoR) = BCR - ABL : ABL ?0.10% by International Scale, on RT - Q - PCR) with imatinib mesylate 400 mg/day at least 1 year. Then used an ELISA technique to assess serum level of sFas, sFas Ligand (sFasL) & Caspase - 3 for each patients of CML, and compared them with 28 apparently healthy volunteers used as control. The controls were age and sex matched with the patients, they were not taken medication and with no history of chronic illness and/or acute infection. As well as, they were nonsmoker and nondrinker. For patients the inclusion criteria including all patients were free of fever and other chronic illness such as diabetes mellitus, hypertension and infection; also they had no history of smoking and drinking of alcohol. And the exclusion criteria including patients suffering from any chronic, debilitating disease and other blood disorders were not taken.The observed results revealed the following : ? Patients were included in the study, male : female ratio was 0.8 : 1with mean age 42.6± 14.0.? The mean± SD serum of sFas levels (pg/ml) for the newly diagnosed and optimally responded of CML was 1163.6±231.5 and 1021.7±360.6 respectively.? The mean± SD serum of sFas Ligand levels (pg/ml) for the newly diagnosed and optimally responded of CML was 216.9±165.7 and 147.7±91 respectively. ? The median serum of Caspase - 3 (ng/ml) for the newly diagnosed and optimally treated cases was 0.421 and 0.361 respectively. ? The mean± SD serum of sFas and sFas Ligand levels (pg/ml) for the healthy group was 970.1±361.7, 152.5±98 consequently. The median serum of Caspase - 3 (ng/ml) for the healthy group was 0.314.? Serum sFas level was increased in newly diagnosed compare with optimal responder and healthy control group. Also serum sFas level in optimal responder group was increase when compared with healthy control group. The differences observed were no statistical significance (P=0.09).? Serum sFasL level was increased in newly diagnosed patients of CML when compared with optimal responder and healthy control group. But serum sFasL level in optimal responder group was decreased when compared with healthy control group. The differences observed were non statistical significance (P=0.07).? Serum Caspase - 3 level showed statistically significant (P ?0.001) increase in newly diagnosed when compared with healthy control group. While it was in optimal responder group was statistically increased (not significant P=0.13) when compared with healthy control group. And its level was increased in newly diagnosed compare with responder group which was statistically significant (P= 0.04).Finally, this study concluded that increase in sFas and sFas Ligand &Caspase - 3 in newly diagnosed CML patients compared with optimally and healthy control groups. Also concluded that Caspase - 3 was a good confirmed test to diagnose newly CML cases

دور بعض المؤشرات الالتهابية المختارة في البول وبروتينات خلية البودوسايت عند مرضى اعتلال الكلية السكري == The Role Of Selected Urinary Inflammatory Markers On Podocyte In Type 2 Diabetic Nephropathy

Author name: داليا محمود خلف
Supervisor name: رفيف صبيح الشوك | عصام نوري الكروي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: اعتلال الكلية السكري هو السبب الرئيسي للمرض الكلوي المزمن وويرتبط ذلك مع زيادة وفيات مرضى القلب والاوعية الدموية. التعريف الكلاسيكي لاعتلال الكلية السكري هو زيادة افراز البروتين في البول. وتتميز المرحلة المبكرة للمرض عن طريق زيادة صغيرة في افراز الزلا | Diabetic nephropathy (DN) is the leading cause of chronic renal disease. It is associated with increased cardiovascular mortality. The DN has been classically defined as increased protein excretion in urine. Early stage is characterized by a small increase in urinary albumin excretion (UAE), also called microalbuminuria or incipient DN. More advanced disease is defined by the presence of macroalbuminuria or proteinuria. The latter is classically named overt DN.This study was planned with the aim of evaluate the levels of urinary Monocyte Chemoattractant Protein - 1(MCP - 1), interleukin (IL - 18), Osteopontin and Nephrin at diabetic nephropathy and its correlation with the parameters in Iraqi type 2 diabetic subjects and to explore the impact of albuminuria and duration of the diabetes on fine glomerular architecture using podocyte injury related marker (Nephrin) in urine. This may explore the more specific, and sensitive early biomarkers for nephropathy in diabetic patients and could help the physicians in controlling the occurrence of renal failure.Sixty Iraqi type 2 diabetes mellitus patients attending the National Diabetes Center for Treatment and Research at Al - Mustansiriyah University during the period October 2014 - June 2017 were recruited for this study. For the purpose of comparison, 20 control subjects matched for age, gender and ethnic background were also included. The patients and controls were characterized in family history of diabetes, diabetic nephropathy groups divide by used Albumin to creatinine ratio. The patients were also assessed for duration of disease, fasting serum glucose, and serum creatinine and blood urea.The mean urinary level of MCP - 1 (15.38±15.50 vs. 6.10±7.23pg/ml) and IL - 18 (35.90±16.67 vs. 23.55±12.67pg/ml) and osteopontin (337.15±257.06 vs. 174.11±183.65ng/ml) and nephrin (100.01±88.66 vs. 52.23±19.61ng/ml) were significantly higher in T2DM patients as compared to controls.The mean levels of urinary MCP - 1, uIL - 18 and osteopontin in macroalbuminurea group of patients were significantly higher than those in normoalbumine ( p < 0.008, p< 0.0001 and p< 0.03 respectively), and only of the Nephrin in the normoalbuminurea group of patients showed significant increased level as compared to the controls (52.23±19.61 vs.75.05±37.8 ng/ml) (p<0.022).The duration of disease showed an increase in mean of urinary level for all the markers MCP - 1, IL - 18, osteopontin and nephrin (17.01±18.09 vs. 19.76±21.30 pg/ml, 29.24±15.98 vs. 42.98±17.25pg/ml, 321.1±291.05 vs. 409.05±210.0ng/ml, 99.40±48.40 vs. 79.21±44.23 ng/ml) respectively, in late duration of disease as compared with early duration of disease except in Nephrin showed the early duration of disease higher than the late duration of disease (99.40±48.40 vs. 79.21±44.23), although the differences were not significant.The Receiver operating characteristic (ROC) curve analysis for all markers MCP - 1, IL - 18, Osteopontin and Nephrin levels (MCP - 1 = 0.72, IL - 18 = 0.71, Osteopontin =0.70 and Nephrin =0.72) for early diagnosis and detection of DN revealed that the cut - off value of uMCP - 1 was 6.23 pg/mg with 70% sensitivity and 75% specificity; whereas, the cut - off value of u IL - 18 pg/ml was 23.69 ng/mg with 80% sensitivity and 59% specificity and Nephrin was 49.5 ng/ml with 78% sensitivity and 55% specificity. uOsteopontin was 182 ng /ml with 68% sensitivity and 69% specificity.The linear correlation revealed a significant positive linear correlation between urinary MCP - 1, IL - 18, Nephrin and A : C ratio, also showed a significant negative linear correlation between urinary MCP - 1, IL - 18, osteopontin and GFR.In conclusion the Nephrin may be considered as potential diagnostic and prognostic biomarker a for the early detection and progression of diabetic nephropathy while the uMCP - 1, uIL - 18 and uOsteopontine may be considered as potential prognostic biomarkers for the diabetic nephropathy. The duration of disease also affect investigated biomarkers suggesting the long term effect of hyperglycemia

تقدير مستويات السايتوكينات (الحركيات الخلوية) المولدة للالتهاب والمضادة للالتهاب لدى مرضى فصال العظام في الركبة قبل وبعد الحقن بالبلازما الغنية بالصفيحات الدموية == Estimation Of Pro - Inflammatory And Anti - Inflammatory Cytokines In Patients With Knee Osteoarthrosis Before And After Injection With Platelets - Rich Plasma (Prp)

Author name: حسنين خضير عبد العباس
Supervisor name: عبد العظيم ياسين عبود البراك | سامر محمد رضا عنون | زيد وجيه رؤوف الشهواني
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: التهاب العظام والمفاصل Osteoarthrosis)) او فصال العظام او ما يعرف بالاسم الشائع السوفان هو مرض يحدث في غضروف المفصل الذي يعمل على عدم احتكاك عظام المفصل ,مما يؤدي الى تقليل هذه الحماية ضد الاحتكاك فتصبح حركة المفصل بها خشونة ومصحوبة بالالام عند استخدام ا | Osteoarthrosis (OA) are defined as a progressive architecture destruction of the joints compared to slow healing of these joints which leads to reduce protection against degeneration and the movement of the joint is associated with by the roughness and accompanied by pain when using the joint. Often this disease affect the knee joint, it may affect any other joint in the body, especially those that carry weights such as leg hinges the pelvis, elbow and spine. The aim of this study is to evaluate the efficacy of platelets - rich plasma injection in osteoarthritis knee and the estimation of the biological and immunological markers associated with the treatment. This study involved 50 patients suffering from inflammation of the knee joint under supervision of speiclist of orthopedic ,most of these patients attended Al - Shaheed Firooz hospital in wasit Governorate and specialty private clinics of orthopedic and Rheumatology in the district during the period from April 2015 until October 2015.The age of patients was about 35 - 65 years; 31 females and 19 males with 25 healthy matching group. All patients were injected into the knee joint by a specialist physician with two injections during two months with platelets - rich plasma (PRP)after its preparation from the same patient's blood in sterile conditions. This was done after the signing of the patient or one of his relatives on the written consent to conduct the injection process after explaining and clarifying the principle of injection and the purpose. The level of pain of patients group was measured by Visual Analogue Scale (VAS). The enzyme linked immunnosorbant assay ELISA method to determine the level of some of cytokines in serum of patients group (interleukin - 1beta, tumor necrosis factor - alpha,IL - 10,IL - 8 and Transforming growth factor - B1) in addition to measuring the concentration of C - Reactive protein (CRP).The rate of Erythrocyte Sedimentation Rate (ESR) was also estimated. All were done to all patients groups before and after injections with PRP in addition to healthy group. Statistical analysis showed no significant differences between males and females (54.5±1.2 males, 53.2±2.1 females). The study showed significant decrease in the mean of the Visual Analogue Scale (VAS) of patients before and after two months of injections with (PRP) 8.46 ± 0.104, 5.98 ± 0.129, , respectively, with highly significant differences (P=000.0) , also the study showed that there was no significant differences in VAS between age groups (30?s, 40?s, 50?s , 60?s) before and after injections with PRP (7.5,8.0 ,8.38 ,9.05), (5.0,5.55,5.69,6.7) respectively. The study also showed a difference in the mean for inflammatory marker C. Reactive protein concentration before and after injections with PRP 7.156± 0.328 µg/ml, 5.384± 0.196 respectively, in comparison with healthy group with highly significant differences (P=0.000).The study also showed a difference in the mean of ESR before and after injections with PRP (35.66± 0.879 mm/1hr, 23.7±0.856) respectively, with in comparison with healthy group highly significant differences (P=0.000).The mean of Interleukin 1 - Beta(IL - 1?) was decline after injections with PRP from 13.220±0.295, pg/ml to 9.622 ± 0.273 pg/ml in comparison with healthy group with highly significant differences (P=0.000). Also a decline in the mean of Tumor necrosis factor - alpha (TNF - ?) after injections with PRP from 62.384±0.927 pg/ml to 55.36 ± 1.121 pg/ml in comparison with healthy group with highly significant differences (P=0.000). The study also demonstrates a decrease in the mean of Interleukin - 8 (IL - 8) before and after injections with PRP (41.092±0.808 pg/ml, 35.93 ± 0.813pg/ml) respectively in comparison with healthy group with highly significant differences (P=0.000). An increase in the mean of Interleukin - 10(IL - 10) after injections with PRP from 5.108±0.291 pg/ml to 6.734±0.330pg/ml, in comparison with healthy group with highly significant differences (P=0.000). The final parameter in this study was demonstrated an increase in the mean of Transforming growth factor - ?1 (TGF - ?1) before and after injections with PRP (111.544±6.960, 149.212±7.540pg/ml) respectively in comparison with healthy group with highly significant differences (P=0.000). Association was obtained between VAS score and C. RP as well as ESR after treatment. PRP treatment also has an impact on increasing the level of anti - inflammatory IL - 10 and TGF - ?1 on one side and the decreasing level of pro - inflammatory IL - 1?, TNF - ?,IL - 8 (catabolic marker) on the other side these results suggest the inhibition of catabolic process demonstrated by reducing of VAS scores and the efficacy of effectiveness plasma rich platelets (PRP) treatment of osteoarthrosis.

دراسة التعبير المناعي النسيجي الكيميائي لبعض المعلمات المناعية في مختلف درجات سرطان البروستات == Study The Immunohistochemical Expression Of Some Immunological Markers In Different Grades Of Prostate Cancer

Author name: حسن هادي زريج
Supervisor name: زينب فاضل عاشور | وسن عبد الاله باقر
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Prostate cancer is the most common malignancy and the second leading cause of cancer related death among males worldwide. Prostate cancer developed in prostate , a gland that is located below the bladder and just in front of rectum.Inflammation is a fundamental physiological process that can arise in any tissue in response to traumatic, infectiousor autoimmune injury. Transforming growth factor beta one (TGF - ?I) is a potential regulator of prostate cancer cell growth that signals through a heteromeric complex composed of type I and type II receptors.TGF - ?R II is an important receptor , because TGF - ? bind to receptor II to initiate it's signaling, then receptor II recruits receptor I to initiate signal transduction. Interleukin - 17 (IL - 17) is a pro - inflammatory cytokine produced by T - helper 17 (Th17) cells contributes toboth the processes by playing a dual role in the antitumor immunity. On one hand, IL - 17 encourages an antitumor cytotoxic T cell response leading to tumor regression. On the other hand, by promoting angiogenesis and egress of tumor cells from the primary focus, so IL - 17 promotes tumor growth.This study was carried out to establish the correlation between expression of Transforming growth factor beta one(TGF - ?I), Transforming growth factor beta receptor one (TGF - ?RI) and Transforming growth factor beta receptor two (TGF - ?RII) and prostate cancer progression. Also to establish the role of IL - 17 in prostate cancer development.The study consisted of 16 patients with poorly differentiated malignancy (PDM) ,whose age ranged (48 - 89) years, with mean age of (71.2) years , and 25 patients with moderately differentiated malignancy(MDM) , whose age ranged (31 - 83) years , with mean age of (66.6) years , and 46 patients with benign prostatic hyperplasia (BPH) , whose age ranged (27 - 85) years , with mean age of (64.3) years. Allpatients were diagnosed surgically by consultant physicians in Baghdad hospital for specialist surgeries , Al - Yarmook teaching hospital ,as well as AL - Hilla teaching hospital , and 20 normal tissue sample taken from cadaver, whose age ranged (17 - 28) years , with mean age of (21.8) years , where chosen as a control group. The work was conducted in Iraqi center for cancer and medical genetic researches. The study were performed during the period from March 2014 to December 2014).Immunohistochemistry (IHC) technique was used to detect the level of expression of TGF - ?I , TGF - ?RI , TGF - ?RII and IL - 17 protein in tissues of patients and healthy control groups.The current study revealed that there was significant difference in mean levels of TGF - ?I protein expression between each of poorly and moderately differentiated malignancy compared with healthy control subjects with P - value of 0.001 , but there was no significant difference in mean level of TGF - ?Iprotein expression between benign prostatic hyperplasia and healthy control subjects with P - value of 0.398. Also there was no significant difference between poorly and moderately differentiated malignancy in mean level of TGF - ?I protein expression.TGF - ?R1 protein was expressed in 3 (18.7%) and 14 (56%) of poorly and moderately differentiated malignancy respectively , with weak immunostaining reaction was the most frequent score. There was significant difference in mean level of TGF - ?RI protein expression among all studied groups. TGF - ?RII protein was expressed in 6 (37.5%) and 22 (88%) of poorly and moderately differentiated malignancy respectively ,with weak immunostaining reaction was the most frequent score. There was significant difference in mean level of TGF - ?RII protein expression among all studied groups.IL - 17 immunohistochemical expression was detected in tissues of all studied groups , the results showed that moderate immunostaining reaction was the most frequent score among them. There was significant difference in mean levels of IL - 17 protein expression among all studied groups.We concluded that there was statistically significant association between the loss ofexpression of TGF - ?1 signaling receptors, especially TGF - ?RI, andincreasing grades of malignancy in prostate cancer. These resultssuggest a potential mechanism for prostate cancer cells toescape the growth inhibitory effect of TGF - ? and thus, leadingto a more malignant phenotype.

دراسة التنميط الجيني لفيروس الSEN في متبرعي الدم الاصحاء ومرضى الثلاسيميا المصابين وغير المصابين بفيروس التهاب الكبد نمط ج واهميته السريرية == Genotypic Study Of SEN Virus In Healthy Blood Donors And Thalassemia Patients; With Or Without HCV Infection And Its Clinical Importance

Author name: بشار منذر خزعل الزبيدي
Supervisor name: اروى هادي الحمداني | اشنا جمال فائق
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Blood transfusion is one of the most common routes of viral hepatitis transmission among population. There are several diseases need continuous blood transfusions to manage the patient's condition; one of these diseases is ?thalassemia, so these patients are at increased risk of infection with blood transfusion transmitted agents. SEN - Virus is a DNA virus which is associated with acute post - transfusion hepatitis and the prevalence with the clinical importance and also the genetic characterization are still much unknown in Iraq; therefore, this study was designed to investigate the occurrence and the clinical importance of SEN - virus infection in healthy blood donors and thalassemia patients with or without HCV infection and then the study of the genome sequencing and the phylogenetic analysis of SEN - V clones. One hundred and fifty eight thalassemia patients (57.6% male, 42.4% female), with mean age of 16.8±8.5 year, and one hundred and fifty healthy blood donors with randomly selected persons (58.7%male, 41.3% female), with mean age of 16.7±8.6 year. All these samples involved in this study that were conducted in the period between January to June 2015. SEN - V had been identified by DNA extraction, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection; while HCV was identified by RNA extraction, RNA reverse transcription into complementary DNA, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection. Liver transaminases (Aspartate Transaminase and Alanine Transaminase) were determined, in addition of measure of serum ferritin levels by VIDAS. SEN - V was detected in 68 out of 158 (43%) thalassemia patients and 16 out of 150 (10.7%) blood donors. HCV prevalence was (11.4%) in thalassemia patients. There was significant increase observed in the occurrence of SEN - V or HCV infection with age but there was no significant difference observed in the occurrence of both with gender. The most important (hepatotropic) SEN - V genotypes were SEN - V - D and SEN - V - H and there were patients infected with D genotype or H genotype alone, while there were patients co - infected with D and H. According to HCV and SEN - V co - infection status, patients and controls subdivided into six subgroups : subgroup I was thalassemia HCV RNA + and SENV DNA +, subgroup II was thalassemia HCV RNA + and SEN - V - , subgroup III was thalassemia HCV RNA - and SEN - V DNA +, subgroup IV was thalassemia HCV RNA - and SEN - V - , subgroup V was control SEN - V + and finally subgroup VI was control SEN - V - . Statistical analysis revealed that there was significant increase in AST and ALT levels in subgroup I when compared with the other subgroups - that showed the increasing relationship in the case of infection with both viruses - and there were no any significant differences in ferritin levels among these subgroups. Moreover, there were no significant differences between SEN - V - D, SEN - V - H and SEN - V - D and H co - infected samples in AST, ALT and ferritin among thalassemia patients and controls. The results from the study of gene sequencing and phylogenetic analysis of samples of amplified SEN - V - D and samples of amplified SEN - V - H DNA which were selected randomly from blood donors and thalassemia patients infected with D or H genotypes alone or together (co - infection), revealed that the most transmission route of SEN - V D and H was blood transfusion that is because there was (99%) gene similarity between blood donors and thalassemia patients, furthermore SEN - V - D or SEN - V - H sequences of the co - infected persons were the same sequences of D or H genotypes alone and with the observations of similarity with neighboring countries.

Immunological Status Of Seminal Plasma For Bodybuilders On Androgenic Anabolic Steroid Hormones == Immunological Status Of Seminal Plasma For Bodybuilders On Androgenic Anabolic Steroid Hormones

Author name: Anwar M. Rasheed Abdulhussein
Supervisor name: Dr.Abduladheem Y. Abbood AL.Barra
General topic: Medicine
Specific topic: Medicine
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Exercises done by bodybuilder have great benefit for the general health and muscle size and performance. Anabolic androgenic steroid hormones are commonly used by bodybuilders to increase muscle mass and strength depending on its mode of action to increase the level of nitrogen retention in muscle, insulin like growth factor IGF - 1 increase in muscle tissue and seminal fluid. IGF - 1 responsible for Cell proliferation, growth and inhibiting apoptosis activity. Also AAS generally has an immunmodulatory impact on cytokines interactions as well as infertility. This study was designed to estimate the changes in cytokines in seminal plasma, sperm count in the seminal fluid and estimation of testosterone concentration in serum due to the impact of androgenic anabolic steroids hormones in bodybuildrs. Eighty male volunteers their age groups are ranging from 21 - 35 years. They were divided into three groups; the first group 27 age matched volunteers apparently healthy (healthy control group) , Second group 27 bodybuilders players attending the sport club with androgen anabolic steroid hormone abuse (AAS) (steroid group), and the third group 26 bodybuilder players attending this sport club in Baghdad without AAS uptake (fitness group ). A blood sample and seminal fluid were obtained from each individual involved in this study. Blood, serum and seminal plasma were prepared from these samples were obtained and applied for estimation of testosterone level by using enzyme immuno - florescent technology. Anti - sperm Ab test by heamo - aglutenation method was used to exclude the positive cases from this study.Heparinized blood was used for estimation of neutrophil phagocytic activity Sperm count was done for each seminal fluid sample, cytokines were estimated by sandwich ELISA method, in seminal plasma, these cytokines are IL - 6, IL - 10,IL17a,TNF - ? and INF - ?. The bodybuilders with AAS uptake showed a significant reduction in sperm count (P=0.00), fitness group sperm count showed slight nonsignificant decrease compared to healthy control group (P=0.7). Serum testosterone level in the AAS uptake group showed a significant decline compared with fitness and healthy control groups (P= 0.00). Exercise alone increases the level of IL - 6 (P=0.00), while exercise with AAS uptake decreases the level of IL - 6 (P=0.06). The fitness group showed highly significant increases in TNF alpha (P =0. 00). IL - 10 as an anti - inflammatory cytokine shows a high level in the AAS uptake group compared to the healthy group (0.04). IL - 17 as an inflammatory cytokine showed high significant increase in steroid group and fitness group (P=0.00), which indicate that both exercises with and without AAS uptake increases the level of IL - 17 secretion(P= 0.02) separately or in combination. Phagocytosis of AAS uptake group showed a highly significant decline (P= 0.00).The correlation results showed that there is a complex network interactions between the studied cytokines. The Phagocytic activity of the AAS uptake group showed a strong correlation with both IL - 6 and IL - 10 levels. IL - 10 as a suppresser cytokine has a significant correlation with TNF - alpha, INF - gamma and IL - 17A while, IL - 10 showed a negative correlation with IL - 6. IL - 17 which act as an inflammatory cytokine has a significant correlation with all cytokines except IL6 and phagocytic activity. Generally seminal plasma cytokine network may be under the effect of immune regulation mechanism and cytokine inactions as well as, the impact of steroids uptakes. In general the final impact of AAS uptake on bodybuilders immunity are declining in phagocytosis and an increase in IL - 10 which act as suppressor to the Th1 immune response and increase of IL - 17 which has a role in increasing the inflammatory response so, these results suggest that AAS abuse players may be more liable for infection and decrease in sperm count which may cause temporary or permanent infertility.

دراسة المقاومة المتعددة للمضادات الحيوية لعصيات التدرن لدى مرضى التدرن الرئوي == A Study On Drug Resistance Of Mycobacterium Tuberculosis Isolated From Pulmonary Tuberculosis Patients

Author name: انمار ليث طالب الحسني
Supervisor name: عامر رشيد النجار | احمد اسمر منخي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: تعتبر عزلات جراثيم التدرن المقاومه للادوية من المشاكل الرئيسية في معالجة مرضى التدرن حول العالم. , ولاهميته اجريت هذه الدراسة لتسليط الضوء اكثر حول الحساسيه الدوائية بواسطة الطرق المختبرية التقليدية وعلى الوسط الصلب ومقارنته مع الطرق التشخيصية المختبرية ا | Drug resistant tuberculosis remained as major problems in the treatment of tuberculosis patients in the world. A prospective study was conducted to evaluate drug susceptibility testing (DST) by classical methods on solid media as compared with advance and molecular Laboratory diagnostic methods (Bactec MGIT 960 and GeneXpert MTB - RIF ). The study included the estimation of the first line anti - TB drugs, including (Rifampicin,Isonizid,Streptomycin and Etambutol) by solid media (Lowenstein - Jensen medium) and Bactec MGIT 960 system. In addition, the use of rapid molecular diagnostic method by GeneXpert MTB/RIF assay. Seventy five TB patients attending the National Reference Laboratory of Tuberculosis /medical city - Baghdad from December 2015 to June 2016 were included in this study. Patients were classified into two main groups : selected MDR patients, which were referred from different hospitals and health respiratory center in Iraq, represented 46 in order to compare different laboratory methods to detect of DST isolates, while 29 were randomly isolated and identified , the study matched by age and gender were also included. This study revealed that the drug resistance tuberculosis to Rifampicin was 47 (62.7%), Isonizid 41 (54.7%), Streptomycin 42 (56.0%) and Ethambutol 38 (50.7%). Respectively , on the golden stander in which solid media were used.The molecular and advance technique (GeneXpert and Bactec MGIT 960) as applied to estimation of drug resistance tuberculosis, the results of drug susceptibility testing (DST), as showed highly sensitivity and Accuracy, when compared with golden stander( LJ media) especially in detected of primary drugs (Rifampicin and Isoniazid) and showed lower sensitivity and accuracy to detected of the secondary drugs (Streptomycin and Ethambutol). The Multiple drug resistance (MDR - TB) case from selective samples was found to be almost similar to that found in other countries of the middle east and other regions in the world. While in the randomly identified samples, the rate of MDR - TB was lower than the referred (previously treated) cases.The GeneXpert MTB - RIF system is rapid molecular methods that used in many countries to identify of MTB and Rifampicin detected during 2 hours only, that used in this study. The high Rifampicin resistance and other drugs of local isolates could be due to abuse of the drug,defaulter and relapse of retreating cases (previously treated).

دراسة التنميط الجيني لفيروس تي تي في مرضى الثلاسيميا المصابين وغير المصابين بفيروس التهاب الكبد نمط ج

Author name: ابتسام نايف عودة الجبالي
Supervisor name: اروى هادي الحمداني
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: ما زال الكثيرون لا يعرفون الاهمية السريرية لفيروس تي تي الذى رشح كاحد فايروسات التهاب الكبد انماط A - G)).ان فيروس تي تي يصيب المرضى المعرضين لخطر الحقن ونقل الدم المتكرر، كما في المرضى المصابين بفقر دم البحر الابيض المتوسط(? - Thalassemia major). تمت هذة | Much is still unknown about the clinical significance of TT virus (TTV) that has been reported as a candidate for non A - G hepatitis virus. TTV infects patients at risk for parenteral exposure and chronic blood transfusion, such as those with ? - thalassemia major. This study was designed to investigate the prevalence and clinical importance of TTV infection in thalassemia patients with or without hepatitis C virus (HCV) co - infection, furthermore to sequence and analyse phylogentic of TTV clones.One hundred fifty four thalassemia patients (64.3% male, 35.7% female) with a mean age of 23.8±6.8 years were involved in this study that was conducted in the period between Feb. to Dec. 2013. TTV DNA was detected using Real time PCR. Furthermore, conventional PCR was done for sequencing and phylogenetic analysis using N22 region from open reading frame 1 (ORF1). Anti - HCV antibody was determined by Enzyme Linked Immunosorbent Assay (ELISA) and confirmed by western blot. Then, HCV core antigen was detected in those anti - HCV Abs seropositive samples. Liver transaminases (ALT, AST) were determined, in addition to measure serum ferritin levels by VIDAS ferritin. TTV was detected in 45 of 154 (29.2%) of thalassemia patients with predominance of males than females (64.4% vs. 35.6%). The prevalence rate of anti - HCV Abs were 54.5% with significant increase in females than males (p<0.05).Out of these 84 samples, 59 (70.2%) were positive by HCV core Ag assay and related significantly to frequency of blood transfusion. According to HCV and TTV infection status, patients were categorized into six groups : Anti - HCVAb+ HCV core Ag + TTV+, Anti - HCVAb+ HCV core Ag + TTV - , Anti - HCVAb+ HCV core Ag - TTV+, Anti - HCVAb+ HCV core - TTV - , Anti - HCVAb - TTV+, Anti - HCVAb - TTV - . The increased levels of ALT, AST and ferritin in the HCV and TTV infected group were not significantly different from those in the TTV and HCV negative groups. Co - infection of TTV and HCV were significantly increase ALT levels compared to infection with HCV alone. No association of TTV infection was found with gender, age and frequency of blood transfusion.The results of genotyping in 12 randomly selected patients showed the presence of equal percentage of genotype 1 (G1) and genotype 2 (G2) 50% for each one, with homology between them 79.0%. Other TTV genotypes were not identified in the present study suggesting that G1and G2 are predominant in this area. Mixed infection of the same patient with multiple TTV genotypes was observed. The results concluded that TTV is moderately present in Iraqi thalassemia patients, with G1 and G2 were predominant. Patients co - infected with HCV and TTV had a significantly higher serum ALT level than those without TTV infection, so TTV infection is suggested to have a role in increasing the severity of liver diseases in the thalassemia patients when co - infected with HCV.

الكشف عن طفر الجين عامل النمو البشراوي الثاني (HER2) في سرطان الخلايا الانتقالية باستعمال طريقة اللونية في موقع التهجين == The Detection Of HER2 Gene Mutation In Transitional Cell Carcinoma Using CISH Method

Author name: عمار عيسى مهدي
Supervisor name: احمد تركي عبيد | هادي محمد علي الموسوي
General topic: Medicine
Specific topic: Clinical Diseases
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Diseases
Language: English
University location: Babylon
First pages:
Abstract: يعتبر سرطان المثانة واحدا من اكثر انواع الاورام السرطانية التي تصيب الجهاز البولي حيث انه يعتبر ثاني ورابع الاكثر شيوعا للذكور في العراق والولايات المتحدة على التوالي وايضا يعتبر العاشر والحادي الاكثر شيوعا للاناث في العراق والولايات المتحدة على التوالي. | The urinary bladder cancers are among the common types of cancer of the urinary tract. It is regarded as the second and the fourth most common cancer types for males in Iraq and in the United State of America (USA) respectively and as tenth and eleventh most common cancer types for females in both Iraq and USA respectively. Urothelial Carcinoma is epithelial tumor of urinary bladder (95% of urinary bladder tumor) and it shows wide range of tumor that begins from papilloma and carcinoma in situ to invasive cancer. The patients with urinary bladder cancer that are presented in non - muscle invasive type or muscle invasive type show different rates of HER2 gene amplification (specially for invasive muscle type). Epidermal growth factor receptor (EGFR, also called HER or type I ErbB tyrosine kinase receptors) family is composed of four receptor member which include HER1 (ErbB1 or EGFR), HER2/neu (ErbB2 or C - ErbB2) , ErbB3 ( HER3), and ErbB4 (HER4). HER2/neu protein is glycoprotein produced by HER2 gene which lies in 17q21 (long arm of chromosome 17). This glycoprotein is normally found in lung, breast, urinary bladder, prostate and stomach, and its cytoplasmic portion has enzymatic function (tyrosine kinase activity). This receptor is responsible for the cell survival, normal cell growing and inhibition of apoptosis. HER2/neu protein overexpression is measured by IHC when the result of IHC is equivocal to HER2/neu protein (+ 2). Then, HER2 gene amplification is measured by using an accurate and more practical CISH method. The aims of this study are tried to detect the percentage of HER2 gene amplification in the urothelial carcinoma and distribution of HER2 gene amplification with clinical criteria (age , sex, grade, stage, and location of tumor) of the patients that had urothelial carcinoma of bladder.This study contained retrospective (30 cases) and prospective (20 cases) and was conducted in Al - Hilla Surgical Teaching Hospital and the period of the study extended from October /2015 to August/2016. Clinical data of patients include age, gender , clinical features, and type of bladder biopsy ( endoscopic resection or cystectomy) are taken with paraffin blocks.The practical part of the study was done in the laboratory of the department of pathology / College of Medicine/Babylon university. It included 50 patients with bladder cancer (35 males and 15 females ) with two groups (10 cases is positive and 10 cases is negative). It included 50 patients who were diagnosed with transitional cell carcinoma of urinary bladder with mean age ± SD ( 66.04 ± 10.148 ) years old ranging between 42 - 83 years old. Also, Positive and negative control slides applied with each run CISH method (+ve control of breast tissue that treated by immnohistochemical procedure ( score +3) and the same tissue were applied by removing HER2/neu probe).Fifty patients who were diagnosed with transitional cell carcinoma of bladder and their biopsies of bladder ( taken from endoscopic biopsy or cystectomy). Then, paraffin - embedding procedure was prepared on tissues biopsy and then, hematoxylin and eosin staining (H. and E. staining) procedure was done. Finally, manual CISH was done on both cases(positive and negative cases) and bladder cancer blocks. This CISH method detected her2 amplification according to ASCO/CAP/2013 scoring system. The results showed that age group 61 - 70 years was the highest positive HER2 amplification (64.7%) among other age groups 51 - 60 years (11.8%) and 71 - 80 years old (23.5%), there was a significant association between these age groups with HER2 amplification , p? 0.05. Sex group displayed that 52.9% of male and 47.1% of female who had positive HER2 amplification and there was no significant difference between sex groups with HER2 amplification, p > 0.05. Other results showed that positive HER2 amplification was highest in grade 3 (88.2%) of transitional cell carcinoma when compared to grade 2 (11.8) and grade 1 (0%) and there was significant association between cancer grading system with HER2 amplification, p? 0.05. MIBC and NMIBC of staging cancer system showed 82.4% and 17.6% positive HER2 amplification respectively with a significant difference between them ( p value ? 0.05). Also, other result of the histological types of transitional cell carcinoma revealed 88.2% and 11.8% (positive HER2 amplification) of papillary and flat shape respectively with a significant correlation between them ( p value ? 0.05). The HER2 gene amplification was correlated with grade and stage of bladder carcinoma which showed high amplification in high grade and stage. The HER2 gene amplification was not correlated with the sex of the patients, but more cases of HER2 gene amplification were recorded in males; while there was a correlation with age group specifically (61 - 70 years old). The HER2 gene amplification was associated with aggressive behavior of blabber carcinoma. The CISH method application in HER2 gene amplification was flexible and applical.

التوصيف الجزيئي لعوامل الضراوة المرتبطة باصابات بكتريا المكورات العنقودية السالبة للتخثير والمعزولة من المصابين بالتهاب المجاري البولية في محافظة بابل == Molecular Characterization Of Some Virulence Associated Factors For Coagulase - Negative Staphylococci Isolated From Urinary Tract Infection In Babylon Province

Author name: سارة هاشم داخل الحلي
Supervisor name: محمد صبري عبد الرزاق السعيد | جواد كاظم الخفاجي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: يهدف هذا البحث الى دراسة بكتريا المكورات العنقودية السلبية التخثر, وشملت الدراسة جمع120 عينة ادرار من المرضى المصابين بالتهاب المجاري البولية من الاشخاص المراجعين والراقدين في مستشفى بابل للنسائية والاطفال ومستشفى الحلة التعليمي وللفترة من كانون الاول 201 | This work aims to study coagulase - negative Staphylococci, which included 120 urine sample were obtained from patients suffering from urinary tract infection; who attained to Babylon Maternity and Pediatrics Hospital, and Al - Hilla General Teaching Hospital, during the period from December 2015 to March 2016), they were diagnosed as having UTI by the Urologists. The samples were analyzed for any signs of infection and immediately inoculated on blood and MacConkey agar media plates. All plates were incubated aerobically at 37 ?C for 24 - 48 hrs.Results of morphological and biochemical characterization revealed that out of total of 120 urine samples, 110 showed positive culture, among them 37 isolates are belonged to coagulase - negative Staphylococci, other 73 isolates belonged to other bacterial genera.Molecular detection of coagulase gene (coa) was done for all CoNS isolates. The results showed that 28/37 isolates (75.67%) were positive for this marker, distributed as follows : S. epidermidis gave 15 isolates, S. saprophyticus gave 9 isolates and S. haemolyticus gave 4 isolates.Also, molecular investigation of fnb A and B genes are carried out for all CoNS isolates by using specific PCR markers. 13/37 isolates (35%) were positive for fnb A gene, as 6/13 of S. epidermidis, 5/13 of S.saprophyticus and 2/13 of S. haemolyticus have this gene in their genome. On the other hand, only 4/37 isolates gave positive result for fnb B gene; as 2 isolates of both S. saprophyticus and S. epidermidis have this gene in their genome.Moreover, sec gene also was investigated in CoNS isolates. The results showed that all CoNS isolates are free from this gene.Regarding urease enzyme production, S. epidermidis and S.saprophyticus isolates were found to be urease positive, while S.haemolyticus isolates display urease negative.To demonstrate the ability of bacteria to form struvite stone, urine samples obtained from healthy individuals were used. It was found that struvite stone was formed by S. epidermidis and S. saprophyticus isolates.Moreover, susceptibility to 10 antibiotics were tested by using disc diffusion test (DDT). S. epidermidis was showed 100% resistance for each Methicilline, Ceftriaxone, Ceftazidime and Cefoxitin. Also, it show high resistance 94%, 88%, 88%, 76%, and 59% for Impenem, Gentamycin, Pipracillin, Ciprofloxacin and Amikacin respectively. However, S. epidermidis showed low resistance (6%) for Vancomycin. So, all S. saprophyticus isolates showed high resistance (100%) to each Pipracilline, Ceftriaxone, Methicillin, Ceftazidime and Ciprofloxacin. It showed high resistance (92%) for each Vancomycin and Imipenem, and (83%), (75%), (75%) for Cefoxitin, Gentamycin and Amikacin respectively. In addition to that, all S. haemolyticus was showed (100%) resistance for each Ceftriaxone, Pipracillin, Methicilline, Ceftazidime and Cefoxitin. Also, showed high resistance (75%), (63%) and (50%) for Gentamycin, Amikacin and Ciprofloxacin respectively. While it was showed low resistance (13%) to Vancomycin.

الصورة البكتيرية والنسيجية المرافقة لالتهاب اللوزتين المزمن والادينويد عند الاطفال == Bacteriological And Histopathological Profile Associated With Chronic Tonsillitis And Adenoid Hypertrophy In Children

Author name: رشا محسن كاظم الحسيني
Supervisor name: حبيب صاحب نهر | صفاء حسين الطريحي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: This study aimed to investigate the bacterial profile of surface and core of infected tonsils and adenoid tissues and histopathological examination of these organs.A total of 122 samples represented by 63 excised tonsils and 59 excised adenoids in addition to 57 blood samples were collected from 70 patients who were referred to Hilla Teaching Hospital (Ear, Nose and Throat unit) in Hilla city within a period of four months from November 2015 to February 2016. Those patients weresuffering from chronic tonsillitis and adenoid hypertrophy. The ages of those patients ranged from 2 to 15 years, 41 (59%) of them were males and 29 (41%) were females.The study included three main parts. The first one was the bacterial diagnosis based on relied diagnostic procedures. Second part was the detection of antistreptolysin O (ASO) by using latex agglutination test, while the third part included histopathological study to detect the pathological lesions that occur during these infections.The results indicated that, the most age group being susceptible for chronic tonsillitis and adenoid hypertrophy was the group of 7 - 10 years, represented 33 patients with a percentage of (47%). Among this group, males were more susceptible with percentage of 20 (29%) compared to female patients13 (19%).The surface of all samples (tonsil and adenoid) revealed positive results for bacterial culture, while in the core of tonsil only 44 samples (70%) were positive for bacterial culture while 19 samples (30%) were negative results (no growth). As in tonsils, the core of adenoid revealed positive results in 32 (54%) samples, while 27 (46%) samples were negative results.The suspected isolates were fully identified by using bacteriological, biochemical and Vitek 2 techniques (for two isolates because of those isolates give variable biochemical test results which confused their diagnosis that lead to using of vitek system technique to diagnosis them, these isolates were rare bacteria that infect tonsil and adenoid). The most common types of bacterial isolates were Staphylococcus aureus which accounted for 75 isolates (23.6%), followed by Streptococcus pyogenes 61isolates (19%), Escherichia coli 57 isolates (18%), Klebsiellae pneumoniae 55 isolates (17.3%), Hemophilus influenza 37 isolates (11.6%), Sphingomonas paucimobilis 14 isolates (4.4%), Staphylococcus epidermides 7 isolates (2.2%), Pseudomonas aeruginosae 6 isolates (2%), Pseudomonas stutzeri 5 isolates (1.6%), and Staphylococcus saprophyticus only one isolate (0.3%).Regarding the bacterial population, there is a difference between tonsils / adenoid surface and tonsil/ adenoid core culture were the most predominant isolate from tonsil surface was Staphylococcus aureus and K. pneumoniae from tonsillar core, while from adenoid surface was Streptococcus pyogenes whereas E. coli from adenoid core.The ASO titer in patients with or without group A streptococcal infections were determined by using latex agglutination test. The results revealed that 8 samples (14%) exhibited positive results, while 49 samples (86%) exhibited negative results out of 57 samples. The results showed statistically no significant differences between patient age groups but showed highly significant differences between males and females.Eight specimens were taking for histopathological examination randomly, four samples were adenoids and four samples were tonsils. The histopathology examination of tonsil and adenoid showed the presence of lymphoid hyperplasia,multiple lymphoid follicles, vascular congestion, fibrosis, germinal centers, infiltration of inflammatory cells, necrosis and apoptosis of tissues

تقييم المؤشرات السريرية وبعض الدلائل الحيوية في مرض دواعم الاسنان == Evaluation Of Clinical Parameters And Some Biomarkers In Periodontal Disease

Author name: امل عليوي حسن
Supervisor name: سعد مرزة حسين الاعرجي | وسام وهاب الحمادي
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: امراض دواعم الاسنان هي مجموعة من امراض الالتهابات التي تصيب التجويف الفموي وتعرض الهياكل الداعمة للاسنان للخطر. تحدث هذه الامراض بسبب عدم التوازن بين الجراثيم المحيطة بالاسنان واستجابة المضيف. يعتبر مرض التهاب اللثة ومرض النساغ المزمن هي من الاقسام الرئيس | Periodontal diseases are a group of inflammatory diseases that affect the oral cavity and jeopardize the supporting structures of teeth. They are caused by an imbalance between periodontal pathogens and host responses. Gingivitis and chronic periodontitis are the major categories of these diseases. The study sample consisted of 80 individual of both genders, their age ranged from 30 to 50 years, and they are divided into three main groups according to the clinical periodontal parameters (plaque index, gingival index, bleeding on probing index, probing pocket depth index and clinical attachment level index). Group ? comprised from 24 (15 female and 9 male) healthy gingival volunteers as a control, group ?? comprised from of 29 (12 female and 17 male) patients with gingivitis, lastly group ??? comprised from 27 (11 female and 16 male) patients with chronic periodontitis. Unstimulated whole saliva samples are collected to determine the levels of Prostaglandin E2, Matrix Metalloprotinase - 8 and Alkaline Phosphatase. Clinical periodontal parameters are recorded at four sites per tooth. The results of present study revealed a highly significant difference (P - value < 0.01) in the mean ± SD for all of clinical periodontal parameters and for the levels of salivary biomarkers (Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase) among chronic periodontitis, gingivitis and control groups. The chronic periodontitis group revealed the higher mean value followed by the gingivitis group and the control group presented the least value. Regarding the correlation between the salivary biomarkers levels and clinical periodontal parameters, the present study showed a significant positive correlation (P< 0.05) and a highly significant positive correlation (P - value < 0.01) between each of salivary biomarker levels and all of clinical periodontal parameters. In addition, this study showed a highly significant positive correlation (P - value < 0.01) between Prostaglandin E2 and Matrix Metalloproteinasee - 8 and a significant positive correlation (P< 0.05) between Prostaglandin E2 and Alkaline Phosphatase and between Matrix Metalloproteinasee - 8 and Alkaline Phosphatase for gingivitis and chronic periodontitis groups. The present study re - emphasized the importance of whole saliva as sampling method in relations of immunological purposes in gingivitis and chronic periodontitis and proposed that the elevated levels of Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase may be one of the host - response mechanisms connect with the clinical appearances of periodontal diseases. Salivary Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase levels may reflected the severity of disease, thus help in the early detection of the disease.

تاثير احمرار الدم الثانوي الدم على وظيفة بطانة الاوعية الدموية الجهازية في محافظة بابل == Effect Of Secondary Polycythemia On Systemic Endothelial Function In Babylon Province

Author name: احمد محي الدين سهراب
Supervisor name: غافل صيهود حسن الشجيري | عامر صاحب المؤمن
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: تغير قطر الشريان العضدي كاستجابة الى الاحتقان , يستعمل بصورة واسعة لتقيم وظيفة بطانة الاوعية الدموية الجهازية بواسطة تسجيل التغير كاستجابة للاحتقان. ضعف او قلة التوسع يلاحظ بصورة واضحة لدى المرضى المصابون باحمرار الدم الثانوي.التوسع المحدث بالمد يقاس كن | Brachial artery diameter variations in response to hyperemia were widely used for the assess endothelial function by recording variations in response to hyperemia. Vasodilation was markedly impaired in polycythemic patients.Flow mediated dilatation (FMD) was measured as the percentage increase in brachial artery diameter at diastole after 5 minute of ischemia.Objectives : Material and MethodsThe present study was conducted at Echo - Doppler department in Marjan medical city / Hilla , in cooperation with the department of physiology of College of medicine / University of Babylon, from November 2015 to April 2016. A history, physical examination, blood pressure, and laboratory were performed on all subjects (patients with secondary polycythemia, and control groups). Seventy subjects were included in the present study. Their ages was ranged between 40 - 60 years. They were divided into two groups. Group 1 : Included 30 healthy subjects without polycythemia, all men, their mean age was 47.20 ± 7.3 years and they served as control group.Group 2 : Included 40 patients, all men, with secondary polycythemia, their mean age 46.90 ± 7.3 years.Method : Measurement of flow - mediated and nitroglycerin - induced dilatation of the brachial artery.Measuring blood pressure, auscultatory method.Assessment of BMI.Laboratory analyses : Serum urea test.Serum creatinin test.White blood cells (WBCs) count : Platelets count.Estimation of packed cell volume (PCV %).Red blood cells (RBCs) count. Hb. level test.Cholesterol test.Triglyceride test.Urea test.Creatinine test.Results : The mean flow mediated dilatation (FMD) on two groups was significantly (p value < 0.01) decreased (16%) in patients group (8%) comparison to control group (24%). The mean Hb concentration was significantly (P value < 0.01) increased in patients group compared to control group.The mean packed cell volume (PCV) was significantly (P vale < 0.01) increased in patients group compared to control group.The mean FMD was found to be significantly (16%) decreased in smoker patients group (8%) compared to nonsmoker control group (24 %).The mean red blood cells count was significantly (P value < 0.01) increased in patients group compared to control group.It was found that triglyceride level significantly increased (P value < 0.01) in patients group compared to control group.There was negative correlation between triglyceride and FMD in patients group. Their are No correlation between age and FMD in patients group.The mean serum cholesterol level was not significantly ( p value < 0.07) increased in patients group compared to control group , while serum triglyceride level, serum creatinin, serum urea were significantly ( p value < 0.01) increased respect in patients comparison to control. Mean glucose, Platelets and red blood cells counts were significantly (P value < 0.01) increased in patients group (polycythemic group) respectively compare to control group respectively.Mean body mass index (BMI) and packed cell volume (PCV) were significant (P value < 0.01) increased respectively in patients group compared to control group. Ages of patients group was non - significantly (P value = 0.89) decreased (46.90 ± 7.00 years) in patients group compare to control group.Conclusions : 1 - The mean flow mediated dilatation (FMD) was significantly decreased (16%) in patients group compared to control group. 2 - The mean Hb concentration , mean packed cell volume (PCV) , red blood cells count, platelets , and body mass index count was significantly increased in patients group compared to control group.3 - Polycythemia as with endothelial dysfunctions that may perform in diagnosis of arterial disease. That used to estimate whereas endothelial dysfunctions result from problem in the cardiovascular system with problem in the prothrombin time.4 - Polycythemia can result in raises causes of thrombosis.Through increase platelet dysfunctions, increase ; white blood cell counts, Hb count, homocystein and cholesterol level as with endothelial dysfunctions.

التاثير الموضعي لعلاج الفيناسترايد في علاج مرض الشعرانية مجهولة السبب == The Effect of Topical Finasteride In Treatment of Idiopathic Hirsutism

Author name: يحيى ابراهيم يحيى
Supervisor name: نسرين جلال محمد البياتي | وسام علي امين
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Medicine
Language: English
University location: Babylon
First pages:
Abstract: يعرف مصطلح الشعرانية هو الشعر الاكثر خشونة وسمكا في النساء مثل نمط والمواقع في الذكور , الاندروجين المسؤولة عن تغير في الصوت وزيادة في كتلة العضلات في المراة هو هرمون التستوستيرون، وانه المسؤول عن الشعرانية، ونظرا لحدوث درجة عالية من هذا المرض في العراق | The term hirsutism defines as presence of coarser, thicker and terminal hair in women in a male like pattern and locations.The androgen responsible for the change in voice and the increase in muscle mass in women is testosterone, and that responsible for hirsutism and due to the highly incidence of this disease in Iraq so the finasteride cream 1% used and the study was carried out in Collage of Medicine / Babylon University from November 2013 - November 2014 for treatment of fifty five females were enrolled in this study. Their age was between (18 - 55 years) and the mean of their age was 32.26. Those females were chosen from Consulting dermatologist department in Mergan medical city in Babylon in which these females were complained from hirsutism and After preparation Finasteride 1% cream and using it by the patients it was measured TSH,FSH,LH and free Testosterone and the follow up every 15 day and after 3 months (end of study) it was measured the same parameters TSH,FSH,LH and free Testosterone and ferryman gallawy scores the patients had two parts : (Pretreatment) the patients with TSH,FSH,LH and free Testosterone level before treated with finasteride cream. (Post treatment) the patients were taking the Finasteride cream and the TSH,FSH,LH and free Testosterone levels measured after three months of treatment with finasteride cream and also measured the : • Hair color• Hair removal frequency of the patients• Pain severity before and after treatment• F - Gallawy score• Visual analogue scoreand the follow up every 15 day and after 3 months (end of study) it was measured the same parameters Finasteride cream 1% had significant decrease in the serum free testosterone levels with no significant effects on others parameters as TSH and FSH and LH level. From the above results we can conclude the following : Finasteride cream 1% is an effective and harmless treatment in patients whom suffer from idiopathic hirsutism.

تاثير معايير النطف ونضوج مادة الصبغين على نتائج عملية الحقن المجهري == Impact of Sperm Parameters And Sperm Chromatin Maturity On Intracytoplasmic Sperm Injection Outcome

Author name: هند عبد الكاظم
Supervisor name: يحيى كاظم السلطاني
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: يعتبر العقم من المشاكل المتعلقة بصحة الانسان ويؤثرعلى سدس الازواج في عمر الانجاب تقريبا. يعد الذكر مسؤلا عن خمسين بالمئه من مجموع الحالات.الوظيفه الرئيسيه لتقنيات الانجاب المعان هي علاج العقم بما فيها حالات العقم الذكري وزيادة فرص الحمل من خلال التغلب عل | Infertility is a health concern affecting nearly 17% of young couples in their reproductive age. Male factor infertility accounts for 50% of all cases. The key responsibility of assisted reproductive technologies (ART) is the management of male infertility by bypassing seminal fluid disorders. Defective Sperm parameters were responsible for deciding the fertility potential and related to pregnancy rate in vivo as well as in vitro.Today, the classic seminal fluid analysis is found to be of limited importance in describing a couple’s fertility status. There is now clinical evidence to show that damage to human sperm DNA may adversely affect reproductive outcomes and the spermatozoa of infertile men possess substantially more DNA damage than do spermatozoa of fertile men and there appears to be a threshold of sperm DNA damage beyond which embryo development and pregnancy are impaired.In view of that, the aims of the present study were : Evaluation of the influence of defective sperm parameters including concentration, morphology and motility on intracytoplasmic sperm injection (ICSI) outcome represented by fertilization rate, cleavage rate, embryo quality and pregnancy rate. Assessment of the sperm chromatin maturity in infertile men using acidic aniline blue stain and compare it with that of men with normal spermiogram. Discovering the relationship between sperm chromatin status and the semen parameters in infertile males. Finding the relationship between sperm chromatin status and ICSI outcome.This study included 60 infertile couples complaining from either primary or secondary infertility attended the clinics of infertility treatment center and underwent intracytoplasmic sperm injection.They were classified according to their spermiogram to normozoospermic group (N), whose female partner had bilateral tubal block, oligoasthenoteratozoospermic group (OAT) and finally, asthenoteratozoospermic group (AT). The female partners of the last two groups had normal reproductive function according to gynecological examination and laboratory investigations. All female partners included in this study were less than 35 years old to minimize the influence of advanced maternal age on ICSI outcome. Seminal fluid analysis were done for all and aniline blue stain was used in this study to evaluate sperm chromatin integrity. The main results of this study showed that infertile couples with impaired sperm parameters show high percentage of positive aniline blue stain i.e., sperm chromatin maturity defect and it is significantly higher than that in couples with normal spermiogram. The best cut off point of positive chromatin percentage associated with pregnancy was 40% i.e., when sperm chromatin abnormality exceeds 40% as detected by aniline blue staining method; the pregnancy rate start to regress. No significant differences in fertilization rate, cleavage rate, embryo quality and pregnancy rate were found among studied groups.No significant difference in ICSI outcome including pregnancy rate between couples with high percentage of sperm chromatin damage ?30% and those with low percentage of sperm chromatin damage < 30% were found. So the main conclusions are : Defective sperm parameters and chromatin immaturity are significantly associated with each other.Forty percent is the threshold of sperm chromatin immaturity after which ICSI outcome deteriorate and depending 30% as a threshold in this study revealed no significant difference in ICSI outcome among studied groups. Intracytoplasmic sperm injection has corrective and therapeutic role for defective sperm parameters and shows a unique capability to treat the most difficult cases of male infertility.

التشخيص الجزيئي لتجرثم الدم لدى الاطفال حديثي الولادة بواسطة استخدام تفاعلات البلمرة المتسلسل في مدينة الحلة == Molecular Detection of Neonatal Sepsis By Using PCR In Hilla City

Author name: نور حامد عباس المرزوك
Supervisor name: الهام عباس بنيان الساعدي | ميساء صالح الشكري
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: في هذه الدراسة, تم جمع 85 عينة دم من 85 مريض مصابين بتسمم الدم (حسب التشخيص السريري الاولي للطبيب), تتراوح اعمارهم بين 1 - 28 يوم من كلا الجنسين (الذكور 53 والاناث 32) للفترة من تشرين الثاني 2014 الى اذار 2015.توضح نتائج هذه الدراسة ان (52,9%) طفلا حديث | Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. In this study, (85) blood specimens were collected from (85) patients with suspected septicemia, age ranged between 1to 28 days from both sexes (53 males and 32 females) during the period from November 2014 to March 2015.The results of this study revealed that (52.9%) are presented with late onset sepsis (infected during 7 - 28 days of their life), while the rest (47.1%) of the neonates are presented with early onset sepsis (infected during the first seven days of their life). The collected samples were investigated for bacterial isolation. Bacterial culture was positive in (7.06%) patients versus (92.9%) patients revealed a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate Staphylococcus aureus (16.67%), one isolate E. coli (16.67%), and one isolate Enterobacter spp. (16.67%).The virulence factors of some bacterial isolates in this study were studied. The capsule was present in all bacteria isolated in this study (100%) except for Enterobacter spp. which did not possess the capsule.Colonization factor antigens were detected in all isolates. It was found that only Klebsiella spp. and E. coli (100%) have the ability to produce CFAI and CFAIII. Whereas Enterobacter spp. and Staphylococcus aureus (100%) have the ability to produce colonization factor antigen I only. The results also showed that all isolates of Klebsiella spp. and Enterobacter spp. did not produce hemolysin, while all isolates of Staphylococcus aureus and E. coli were able to produce hemolysin.Extracellular protease was also investigated. It was found that (100%) of all bacteria isolated in this study were able to produce extracellular protease.Biofilm formation was investigated in all bacterial species, and the results showed that all isolates of Gram negative bacteria and Gram positive bacteria form a biofilm.The ability of some bacteria to produce bacteriocin was tested and the results showed that E. coli and Klebsiella spp. were able to produce bacteriocin.In - vitro, the susceptibility of bacteria towards some antibiotics included in this study revealed that the amikacin and kanamycin are the most effective antibiotics to different types of pathogenic bacteria. Finally, molecular detection for bacterial sepsis was done by using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in (20%) of samples and rpoB gene present in (18.8%). While its gene used for detection of fungi revealed negative results in all samples.

تقييم جهاز كتلة غاز الزفير كوسيلة لفحص وظائف الرئتين عند العراقيين الاصحاء == Evaluation of Expiratory Gas Mass Device As A Measure of Lung Function Test In Healthy Iraqi Subjects

Author name: نبراس حسين عبد الساده الحسناوي
Supervisor name: يسار محمد حسن الشماع | باسم متعب هادي زوين
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: تعد فحوصات وظائف الرئتين من الوسائل المفيدة جدا لاغراض التشخيص والمتابعة والبحث العلمي للاصحاء ومرضى الجهاز التنفسي. وابسط تلك الفحوصات واكثرها معلومة هو الفحص السبايرومتري الذي يتضمن قياس السعة الحيوية القسرية (FVC) , وحجم الزفير القسري في الثانية الا | Lung function tests are very useful diagnostic, follow up and research measures for healthy and respiratory compromised subjects. The simplest, yet the most informative, lung function tests are the spirometric tests.They involve the forced vital capacity (FVC), the forced expiratory volume in first second (FEV1) and the forced expiratory volume ratio (FEV%). Several types of devices are used to perform spirometric tests based on the measurement of lung volumes and their subdivisions. Lung volumes, hence, are represented by the volume of expired air. There was a controversy about the difference between body and ambient temperatures which may extremely affect gas volume , but this is thought to be solved by temperature correction. Temperature correction, however, may not be the precise solution in extremely fluctuating environmental temperatures. A fixed amount of expired gas has a constant mass despite the changes in pressure or volume as is implied by the law of conservation of mass in a closed system by Antoine Lavoisier. The aim of this study is to construct and evaluate a simple device called expiratory gas mass device as a reliable, reproducible, accurate and convenient tool to substitute Vitalograph spirometer as a measure of lung function in healthy Iraqi subjects and testing the hypothesis that expired gas mass could significantly and acceptably alternate or conjugate its volume as measures of lung function. The design of the study is a cross sectional study. The statistically required number of participants was decided by a professional statistician to be 70 healthy subjects. However, 300 healthy subjects are included in the present research to cover the need for subgrouping (146 male and 154 female) aged 20 - 50 years (31.84 ± 9.28 years). The male and female were classified into three age groups for each one, ten years interval was considered to be suitable for classifying subjects.Subjects with abnormal lung function such as smokers were excluded. A uniform case sheet was assigned for every participant in the study which includes, name, gender, age, weight, height, smoking habits, residence, cultural status, socioeconomic status, present and past medical history, family history of respiratory illnesses. They were asked to perform the forced expiratory maneuver through the standardized spirometer and then through the newly constructed gas mass device.The accuracy (systematic error) and reproducibility (random error) were tested for the constructed gas mass device.A comparison between forced vital capacity (FVC) and adjusted gas mass (AGM) was made on two main groups , the male group and female group, both of them were subdivided to three age groups.The results revealed that there were no significant differences in values of FVC and AGM measured by standard Vitalograph spirometer and gas mass device respectively. Regression lines indicated that there were strong positive correlations between gas mass (GM) and FVC in male and female. Separate data for male and female are processed and the results indicated that FVC values and GM (or AGM) values are significantly higher in male than in female , P?0.05. Regression lines indicated that there was a negative correlation between age and both of FVC and GM, a positive correlation between height and both of FVC and GM and a weak positive correlation between weight and both of FVC and GM. From the present study, it can concluded that gas mass device is simple, portable and easily handled device. It is also reproducible and accurate device for the measurement of GM in normal subjects.

اثر عوامل البيئة الاجتماعية على سلوك سيطرة وزن المراهقين في المدارس الثانوية في مدينة بغداد == Impact of Socio - Environmental Factors Upon Adolescents' Weight Control Behaviors In Secondary Schools At Baghdad City

Author name: بثينة بشير صالح
Supervisor name: اقبال غانم علي معلة
General topic: Medicine
Specific topic: Nursing
Degree: Doctorate
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: دراسة وصفية(مقطعية) اجريت في جانبي الكرخ والرصافة لمعرفة اثر عوامل البيئة الاجتماعية على سلوك سيطرة وزن المراهقين في المدارس الثانوية في مدينة بغداد للفترة من 20 من شهر نيسان لعام 2013 الى نهاية تشرين الاول لعام 2014.تهدف هذه الدراسة للتعرف على عوامل ال | Overweight and obesity are one of the biggest threats for public health, both in children and adolescents. Fair enough, a lot of attention is paid towards this threat. However, hardly any attention is paid to weight loss behavior in normal weight children and adolescents. Teenagers who consider themselves as too fat who were negative body image a high risk for unnecessary weight loss behavior. A Cross - Sectional Descriptive study was conducted on impact of socio - environmental factors upon adolescents' weight control behaviors in secondary schools at both sides Al - Karkh and Al - Risafa sectors of Baghdad city. The study period started from 20th of April 2013 to the end of October 2014. The study aimed to find out the relationship between weight control, and socio - environmental factors and adolescents' demographic data (age, gender, Body Mass Index, and socio - economic status), and find out the impact of socio - environmental factors upon adolescents' weight control behaviors. A non - probability (purposive sample) of secondary adolescent schools , while 1254 adolescents' students were chosen randomly from that schools. The study instruments consist of four parts. The first part concerned with adolescents' students and their parents? socio - demographic data; the second part is about adolescents' students eating patterns and their meals list at home. The second part is divided into six sections. The 1 section consists of 28 items : the adolescents' students eating patterns and meals list at home, section 2 and 3 contains 16 items : for eating the snacks and fast food, section 4 is composed of 12 items : which are about adolescents' students eating time in schools with peers, section 5 has 12 items : related to the reasons of skipping meals, and section 6 consists of 6 items : about adolescents' lifestyle. The third part is about the physical activities and dieting programs, it consists of 7 items. The fourth part 10 items are related to the adolescents' weight control behaviors. Test - retest determined the reliability of the study instrument which was r = 0.90. A descriptive statistical analysis (frequencies and percentages) and inferential statistical analysis, and (chi - square) were used. The result of the study sample indicated that more than two fourth of the Body Mass Index was almost equal in under or normal weight while the another half were overweight and obese, and the sample's family socio - economic status was in high or low level status. The study indicated most of the adolescents' took breakfast at home, eating snacks, reasons of skipping breakfast meal, and their lifestyle had a highly significant association with their gender and that indicate no differentiate between their gender eat what they want at any time. The results of the study reflected the adolescents' students eating lunch and dinner at home, and reasons of skipping breakfast meal had no significant association with their ages. The adolescents' students favorite to eat snacks and fast food at home and it's had a highly significant association with their Body Mass Index. Also there is a highly significant associated for the study sample socio - environmental factors with their socio - economic status. The results of the study reflect that is a highly significant association between eating habits and their total weight control behavior methods. The study recommended the Ministry of Health need to activate the nutritional program strategies for weight management within school health services, and need to increase establishment other especial centers for managing adolescents from risk for overweight and obesity like as Obesity Research and Therapy Unit in AL - Kindy College of Medicine and Nutrition Research Institute. I suggest a schedule as a guideline for healthy nutritious for weight control and achieve daily physical activity Finally Ministry of Education need to involved teachers in that strategies and training them in relation to these programs.

عزل بكتريا Bacillus cereus من بعض عينات الغذاء == Isolation of Bacillus Cereus From Some Foods Samples

Author name: ضحى عبد الله كاظم الظويهري
Supervisor name: حبيب صاحب نهر
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: كائن Bacillus cereus موجود في كل مكان وهوممرض منقول بالاغذية والذي يمكن ان يسبب نوعين من امراض الجهاز الهضمي : القيء والاسهال. في هذه الدراسه, تم جمع 140 عينه غذائية شملت (الرز, اغذية الاطفال، الدقيق، منتجات الالبان، اللحوم، الحليب , التوابل) جمعت عشوائيا | Bacillus cereus is a ubiquitous organism and a potential foodborne pathogen that can cause two types of gastrointestinal diseases : emesis and diarrhea. In this study, a total of 140 food samples included (rice, infant food, flour, dairy products, meat, milk powder and spices) were collected randomly from restaurants, food stores and various sale points in the local markets of the Hilla province during the period of November 2014 to April 2015 in attempt to isolate of Bacillus cereus from these samples and detection the prevalence of diarrheal (hbl and nhe) and emetic (ces) enterotoxins genes by using Polymerase Chain Reaction (PCR). All bacterial isolates were identified by cultural, microscopic and biochemical examinations. The results showed that 56 (40%) of samples showed positive growth for Bacillus cereus. The antibiotic sensitivity test of some isolates to 10 antibiotics was studied. It has been found that all B. cereus isolates were resistant to Ampicillin, Ampiclox, Cefotaxime, and Cephalexin, while all isolates were sensitive to Gentamycin, Ciprofloxacine and Chloramphenicol, but most of them showed different rate of sensitivity to one or more of antibiotics such as Neomycin 90% and 80% for both Tetracycline and Erythromycin. Polymerase Chain Reaction technique (PCR) was used to detect the ability of bacterial isolates to encode two types of enterotoxin genes, diarrheal and emetic genes. The results showed that 4(7.14 %) of isolates harboured hblA gene, 16 (28.57%) hblC, 5(8.92 %) hblD, 46(82.14%) nheA, 24(42.85%) nhe B, 26 (46.42%) nheC and 3(5.35%) of the isolates harboured Ces gene. On the other hand, through using PCR it was found that nhe gene was present in most isolates, while hbl gene was variable among the studied isolates. Moreover, the Ces gene was found only in isolates being isolated from rice samples, while it was not found in other types of samples.

تاثير العلاج بعقار الميثوتركسيت على عامل النمو البطاني الوعائي، والعوامل الدموية ومضادات الاكسدة في الصدفية == Effects of Methotrexate Therapy On Vascular Endothelial Growth Factor, Hematological And Antioxidants Parameters In Psoriasis

Author name: شكرية حاتم علوان
Supervisor name: محمد عبيد المحمدي | محمد كاظم الحطاب
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: مبدا الدراسة : الصدفية مرض جلدي شائع ذو علامات جلدية ظاهرة، يتميز بالتهاب مزمن للجلد مع اختلاف في نضوج الخلايا الكيراتينية، التي تزيد من سمك الجلد. عملية تكوين الاوعية الدموية (Angiogenesis) هي المسؤول بالدرجة الاساس عن تطور افة الصدفية، واحد اهم عوامل تكو | Background : Psoriasis is a common skin disease with extra - cutaneous manifestations. It characterized by chronic inflammation of the skin with changes in the maturation of keratinocytes, which manifested by the hyperproliferation of the epidermis. Angiogenesis process is a central regulator of development psoriasis lesion and one of the important angiogenesis factor is vascular endothelial growth factor (VEGF).A major site of oxidative stress occurs in the skin due to reactive oxygen species, which play an important role in inflammatory skin diseases such as psoriasis. Objectives : The aim of this presented study is to evaluating the effect of MTX drug on psoriatic lesions by calculating psoriasis area and severity index (PASI) for three months. In addition, investigate the changes of the level of serum VEGF, antioxidants (GSH and SOD) and lipid profile, which include (TC, TG, HDL, LDL and VLDL). As well as, hematological assessment such as (RBCs, Hb, PCV, RBCs indices, ESR and WBCs) in patients with psoriasis before and after (one and three months) from treatment with oral methotrexate therapy in comparison to their levels in healthy control persons. Materials and Methods : This study was a cohort study in which a total of 80 subjects, half of them(40) with psoriasis (25 male and 15 female) had selected randomly from Marjan Medical City in AL - Hilla city and the other half (40)as healthy controls (21 male and 19 female). These subjects collected during the period from 28th/ October /2014 until 15th/ August/ 2015. Physical examination (body mass index) done to each subject that enrolled in the study. A clinical assessment of psoriasis disease activity in psoriasis patients and methotrexate efficacy was done with the Disease Activity Index (DAI) represented by PASI score. Blood collection was obtained from all psoriatic patients and control to use in determine the level of all biochemical parameter such as VEGF, GSH SOD and lipid profile , as well as hematological investigation which include RBCs, Hb, PCV, RBCs indices, ESR and WBCs. Result : The results of this work showed, The effect of systemic methotrexate therapy on psoriasis lesion by significant reduction of PASI levels after treatment and by a significant mean difference in psoriasis area severity index before and after (one and three months) from treatment in psoriatic lesions (P?0.05). Also, no significant differences (p> 0.05) between the mean of age for psoriatic patients group (male and female) and healthy control group (males and females). The body mass index shows significant increase (P?0.05) in psoriasis patients than control group. The serum of VEGF level was significant increase in patients with psoriasis when compare with control through study periods, and there was significant reduction in the level of VEGF in psoriasis patients as compared its level before and after treatment with MTX for one and three months (P?0.05). While, the level of GSH and SOD significant decrease in patients than control ,during the treatment with MTX ,there was significant difference increase in the level of GSH of patients as compare with baseline level (before treatment) (P?0.05). In addition, the level of SOD of patients appear there was no statically difference before treatment and after treatment with MTX. The results of the present study also show a significant increase (P?0.05) in total cholesterol(TC), triglycerides(TG), low density lipoprotein - cholesterol (LDL) and very low density lipoprotein - cholesterol(VLDL) concentration, while found a significant decrease (P?0.05) in level of HDL in serum of psoriasis group compared with those of the healthy control group. When compare between patients during follow up periods, found there was no significant different of level of TC, TG, LDL and VLDL between psoriasis patients in period before treatment when compared with after one months from treatment with MTX (P>0.05). While there is a significant increase between patients before treatment compare with after three months, and after one month from treatment compare with after three months from treatment with MTX (P? 0.05). The level of HDL in psoriatic patients was significant increase at (P? 0.05) before treatment as comparing with after (one month and three months) from treatment with MTX, but there is no significant difference between patients after one from treatment as compared to with after three month from treatment with methotrexate (P> 0.05). The result of hematological for the results of study showed , there is a significant decrease (P? 0.05) in RBCs counts, Hb, PCV and RBCs indices compared with healthy control. While significant increase in total WBCs counts and ESR in psoriatic patients in all periods of follow up periods as compare with control. When compare the RBCs, Hb, PCV, RBCs indices of patients in all periods before and after (one and three months) from treatment with MTX, found there is no significant difference among them at (P> 0.05). While the result of WBCs count show significant decrease (P?0.05) between patient before treatment and after three months from treatment with MTX , and between after one month from treatment with MTX as compare with after three month from treatment , but there is no significant difference between before treatment compare to after one month from treated with MTX. The results of ESR of psoriasis patient show significant decrease (P? 0.05) between before treatment as compare with after three months from treatment with MTX , and after one month from treatment compare to after three month from treatment.While there is no any significant difference between patient before treatment compare with after one month from treatment with methotrexate (P>0.05). Conclusion : The vascular endothelial growth factor have play important role in the evaluation of psoriasis severity, oral methotrexate therapy have efficacy upon serum levels of vascular endothelial growth factor. Efficacy of oral methotrexate therapy on psoriatic patient’s treatment, have there was faster remission of psoriatic lesions and this reflect the immune - modulatory anti - inflammatory role of The MTX in psoriasis patient.

دور تخطيط العضلات احادي الليف على مرضى اعتلال الاعصاب المحيطية السكري وعلاقته بمؤشرات الاكسدة == The Role of Single Fiber Electromyography In Patients With Diabetic Neuropathy And Its Relation To Oxidative Stress Parameters

Author name: زاهد محمد علي كاظم
Supervisor name: احسان محمد عبود عجينة
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: الخلفية : يعتبر اعتلال الاعصاب المحيطية السكري من اكثر مضاعفات داء السكري شيوعا التي تؤثر على الاوعية الدموية الدقيقة. سريريا يؤثر المرض اولا على الاطراف القصيه مؤديا الى فقدان الاحساس في تلك الاطراف على نمط الجورب والقفاز. يؤثر المرض على كل من الالياف ال | Background : diabetic peripheral neuropathy (DPN) is the most common microvascular complication of diabetes mellitus (DM). Clinically, it affects the most distal extremities first resulting in a stock and glove pattern of sensory loss. It affects both small fibers (myelinated and un - myelinated) as well as the large myelinated nerve fibers. However, the earliest manifestations might be due to small fiber dysfunction. Hyperglycaemia and subsequent metabolic derangement is the main pathogenitic factor of DPN. However, oxidative stress, deficiency of neuroprotective factors and neurovascular insufficiency might play a role.AIMS : This study aims to : 1. Test the value of applying SFEMG in the diagnosis of diabetic peripheral neuropathy. 2. Determine the usefulness of SFEMG, compared to the standard NCS, in the early diagnosis of diabetic peripheral neuropathy. 3. Evaluate the correlation of the antioxidant/oxidant status in patients with and without diabetic peripheral neuropathy.Materials and methods : The study was conducted in the period from Nov. 2012 till Apr. 2014 and included 188 patients with type 2 DM that fall within age group of 45 - 65 years. Diabetics that suffer from clinical features of peripheral nerve dysfunction documented with abnormal electrophysiological findings were considered as patients with DPN (138 patients) and those who had no complaint of peripheral nerve dysfunction with normal electrophysiological study were considered as patients without DPN (50 patients). The study also included normal healthy persons who their age and gender matched that patients and considered as control subjects (50 persons). The patients and control enrolled in the study had undergone comprehensive assessment that included : clinical assessment (history and full examination), biochemical assessment (measurement of fasting blood sugar, glycated haemoglobin, total antioxidant capacity and advanced glycation end products) and electrophysiological assessment that included sensory and motor nerve conduction studies (NCS) with single fiber electromyography (SFEMG).Results : The study found that patients with DPN in comparison to patients without DPN and control subjects are older (p?0.05), had longer duration of DM (p?0.01), poorer glycemic control reflected by fasting blood sugar (p?0.01) and glycated haemoglobin (p?0.01), lower level of total antioxidant capacity (p?0.01) and higher advanced glycation end products level (p?0.01). Also, the study showed that the level of total antioxidant capacity and advanced glycation end products on one hand were correlated with the duration of the diabetes (p?0.01) and not correlated with glycated haemoglobin level on the other hand.The electrophysiological findings showed that patients with DPN had significant differences in NCS parameters when compared to patients without DPN and to control subjects in the form of axonal degeneration and demyelination with abnormal single fiber jitter and fiber density which were correlated with the duration of diabetes (p?0.01) but not correlated with glycemic control. Also the study demonstrated that SFEMG findings were correlated with NCS parameters (namely sural nerve) in patients with DPN. This study had concluded that the duration of diabetes, age, glycemic control and oxidative stress are important risk factors of DPN. Routine NCS is important mean for diagnosing DPN but it has limited sensitivity in the diagnosis of early stages of the disease and in assessing nerve degeneration and regeneration. Sensory nerves of lower limbs are helpful in discovering early stages of DPN when other tested nerves are normal. SFEMG examination in diabetic patients is important in showing the degree of re - innervation and nerve regeneration and in identification of subclinical nerve dysfunction.

دراسة العلاقة النسبية بين جريان الدم المخي - المشيمي والرحمي - المشيمي بالدوبلر للنساء الحوامل المصابات بداء السكري == A Study of Cerebro - Placental And Utero - Placental Ratio Doppler Velocimetry In Diabetic Pregnant Women

Author name: رياض وحيد العيساوي
Supervisor name: عقيل عبد المنعم حسن زوين
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: يعتبر داء السكري المصاحب للحمل هو احد مضاعفات الحمل الاكثر شيوعا والتي ترتبط بمعدلات هامة من الاعتلال والوفاة ما حول الولادة , ان العلاج الفعال لداء السكري ما قبل الحمل ولداء السكر المصاحب للحمل قد تبين تحسين النتائج والحد من وفيات ما حول الولادة بالمقارن | Diabetes mellitus complicating pregnancy is one of the most common antenatal complications that are associated with significant perinatal mortality and morbidity(Magee et al., 1993, Schmidt et al., 2001and Platt et al., 2002). Effective treatment of pre - existing as well as gestational diabetes mellitus was shown to improve outcome and reduce perinatal mortality, as compared to untreated patients (Lao TT, et al., 2001and Langer , et al., 2005). Doppler studies have conventionally been utilized for fetal surveillance. The use maternal uterine artery Doppler as a screening tool to predict subsequent development of pre - eclampsia and fetal growth restriction in later gestation have gained acceptance in recent years. Umbilical artery Doppler, middle cerebral artery Doppler have been widely used for the assessment of growth restricted fetuses. The role of Doppler study in diabetic pregnancies has not been unequivocally evaluated. An atherogenic factor, that promotes vascular endothelial growth, insulin - like growth factor - 1 has been implicated with micro vascular complication during pregnancy (Kathryn 2000). Presently, short - term beneficial metabolic effects of recombinant human insulin - like growth nfactor - 1 (rhIGF) - 1 therapy have been demonstrated in numerous diabetic conditions, including type 1 diabetes mellitus, type 2 diabetes mellitus. Until recently, insulin - like growth factor - 1, based upon its endothelial growth promotion, was considered a mediator of vascular disease( Bayes - genis et al., 2001a; 2001b). Conversely, increasing evidence indicate the protective mechanism of insulin - like growth factor - 1 against vascular derangements, relating to its ability of nitric oxide production (Okura et al., 2001). We sought to investigate the impact of insulin - like growth factor - 1 levels on maternal/fetal blood flow in diabetic non - hypertensive pregnant women; an avenue of research that has yet to be fully explored. Taken together, this information will materialize the intervention needed to prevent or at least ameliorate diabetes maternal and fetal outcomes. OBJECTIVES1 - To evaluate effect of diabetes mellitus on utero - and cerebro - placental Doppler velocimetry in pregnant women, such as resistive index (RI), pulsatility index (PI), Systolic/diastolic ratio (S/D)2 - To find whether a meaningful correlation exists between the above mentioned parameters and the levels of HBA1c and maternal IGF - 1.3 - To predict the most sensitive parameter detecting complications of diabetes mellitus on fetal outcomes. MATERIAL AND METHOD In this prospective cohort study a total of 190 pregnant women with single viable fetus, cephalic presentation, were enrolled after having their verbal and written consent and approval of the ethical committee at Faculty of Medicine. They were divided into two Groups : group (Ia) women with gestational diabetes mellitus (n=70), group ( Ib ) women with pre - gestational diabetes mellitus (n=64) and the remaining (n=56) participants (group II) were free from diabetes and hypertension and served as a control group. The study performed at Al Ameer Diagnostic Center in Al Najaf, between March, 2013 and August, 2014.Examination of the participants done twice, in the second and third trimesters. In each time, history and clinical examinations performed with Doppler ultrasonography of the pregnant women including interrogations of bilateral maternal uterine arteries, fetal umbilical and middle cerebral arteries in addition to measurement of IGF - 1 and HbA1c concentrations. RESULTS AND CONCLUSIONS 1 - IGF - 1 mean± SD in second trimester was 582.61± 183.7 ng/ml in group Ia, 429.5± 231.3 ng/ml in group 1b and 278.6 ± 60.8 ng/ml in the group II with significant difference among the groups (P=0.0001) In the third trimester the mean± SD was 653.69 ±197.6 ng/ml in group Ia, 487.5± 242.4 ng/ml in group Ib and 316.6 ± 60.9 ng/ml in group II with significant difference among the groups (P=0.0001).2 - HbA1c mean± SD in second trimester was 6.59± 0.89 % in group Ia, 6.42 ± 0.9% in group Ib and 5.35 ± 0.56 % in group II with significant difference between group Ia and group II, as well as between Ib and group II, but no significant difference between Ia and Ib groups.Third trimester HbA1c mean value was 6.29 ± 0.78 % in group Ia , in group Ib 6.27 ± 0.87 % and 5.08 ± 0.57% in group II.There was no significant difference between group Ia and Ib (P=0.843) , while there was a significant difference between group Ia and group II as well as between group Ib and group II(P=0.0001).3 - Uterine artery Doppler velocimetry was considered more sensitive predictor than other arteries in evaluation of fetal outcomes and its sensitivity in the total studied population (190) was 44.3%, in gestational diabetic pregnancy group was 58.3%, in pregestational diabetic pregnancy 34.3% and in control was 29.4%.4 - Multiple maternal and fetal arterial Doppler studies at same time yield high predictive values in evaluation of fetal outcomes with general sensitivity in the total studied population was 63.6%, specificity 79.4%79, PPV 72.2% and NPV 71.7%. In gestational diabetic pregnancy the sensitivity was 94.4%, specificity was 61.8%, PPV was 72.3%, NPV was 91.3%. In pregestational diabetic pregnancy sensitivity was 88.6%, specificity was 86.2 %, PPV was 88.6 %, NPV was 86.2%. In control healthy pregnancies the sensitivity was 70.6 %, specificity was 71.8%, PPV was 52.2%, NPV was 84.8 %.

تاثير التقنيات المساعدة على الانجاب على نتاج الحمل في مستشفيات بغداد : دراسة مقارنة == Impact of Assisted Reproductive Technology On Pregnancy Outcome In Baghdad Hospitals : Comparative Study

Author name: رغد زغير دوسر
Supervisor name: عز الدين فخر الدين بهاء الدين
General topic: Medicine
Specific topic: Pediatrics
Degree: Master
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: الخلفية : في عام1978 ولادة الطفل الاول في المملكة المتحدة من قبل تقنيات المساعدة على الانجاب، ازداد كلا من التقنيات المتطورة التي تستخدم لعلاج العقم وعدد مراكز الاخصاب التي توفر خدمات التقنيات المساعدة على الانجاب بشكل كبير في كثير من البلدان,وتشمل التقنيا | Background : In 1978 the first baby born in the U.K by the techniques of assisted reproduction. both the use of advanced technologies to overcome infertility and the number of fertility clinics providing ART services have increased steadily in many countries. ART includes fertility treatments in which both eggs and sperm are handled in the laboratory (in vitro fertilization (IVF), intra cytoplasmic sperm injection (ICSI), gamete intrafallopian transfer (GIFT), zygote intrafalopian transfer(ZIFT), and related procedures). Women who undergo ART procedures are more likely to deliver multiple - birth infants than those who conceive naturally because more than one embryo might be transferred during a procedure. Multiple births pose substantial risks to both mothers and infants, including pregnancy complications like, preterm delivery, and low birth weight infants. Caesarean section, pregnancy induce hypertension, gestational diabetes, congenital malformation.Objective : To assess the impact of assisted reproductive technology on pregnancy outcome and compare them to those of natural conception. Methodology : The present study employs a descriptive - purposive design carried out for pregnant women after assisted reproductive technology attending labor wards of Baghdad Hospitals, Kamal AL - samarae Hospital, Baghdad Teaching Hospital, AL Imamain AL - Kadhmien Teaching Hospital, Private Nursing House Hospital, AL - Elwya Maternity Teaching Hospital, AL Imam Ali Hospital, AL - Karkh Maternity Hospital for, and Ibn AL Baladi Hospital. The period for collecting the data was four months (1st February 2014 to the 1st Jun 2014). The study sample divided into two groups : 100 women conceived after assisted reproductive technology(study group), 100 women conceived naturally (control group). Validity through a panel of (15) experts and the reliability of the questionnaire is determined through the pilot study. For the purpose of the present study a questionnaire format was constructed. The data has been managed and analyzed by using descriptive and inferential statistics. Results : An increased pregnancy complication as well as higher risk of obstetrical, perinatal, and neonatal complications was observed in ART compared with natural pregnancy included in this study. The women conceived by ART were older than those conceived naturally (30.86_+ 4.76 vs 25.85 - +7.78) ART pregnancies were associated with a higher risk of early pregnancy bleeding (8% vs 42%), antepartum heamorrhage (4% vs 13%), placenta previa (6% vs 15%), pregnancy - induced hypertension (29% vs 46%), gestational diabetes (7% vs 20%), preterm rupture of membrane (11% vs 25%), preeclampsia (3% vs 12%), obesity during pregnancy (47% vs 65% ), preterm delivery (16% vs 48%), cesarean section( 67% vs 100%), low birth weight (12% vs 32.5%), multiple birth (twin 11% and twin tri 7% vs.0.0%), the period of the newborn child stay in the neonatal intensive care unit (15 days 47.8% vs. 0.0%)Conclusion : The pregnancies after ART are associated with higher risk of multiple pregnancies, obstetrical, perinatal, as well as neonatal complications in comparison with natural pregnancy. Recommendations : Educate mothers who become pregnant using the techniques of assisted reproduction importance they received of their children examined periodically, with long - term developmental follow - up on a regular basis, to exclude late complication of the pregnancy outcome. Emphasis should be on the birth of healthy infants primarily using Embryo single transfer (EST),to reduce the incidence of low birth weight and preterm delivery.

تقويم التعلم الموجة الذاتي للممارسات المختبرية المتعلقة باعطاء الدواء لطلبة كليات التمريض في جنوب العراق == Evaluation of Students' Self - Directed Learning Laboratory Practices Related To Administration of Medication In Southern of Iraq Nursing Colleges

Author name: حيدر علي حسين الوحيلي
Supervisor name: نرمين بدري توفيق البارودي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: المقدمة : الصعوبات التي تواجه تعليم التمريض تكمن في انتاج القوى العاملة التمريضية وهذا يتطلب رعاية الطلاب بالكفاءات اللازمة التي من شانها تسهيل انتقالهم من طلبة التمريض الى ممرضين كفوئين مؤهلين في وظيفتهم. حيث ان للتعلم الموجه الذاتي اهمية كبرى في تطوير ق | Background : The challenge in nursing education lies in the production of a nursing workforce. This requires nurturing the students with the necessary competencies that will facilitate their transition from nursing students to qualified staff nurses. Self - directed learning is of great importance to the professional development of nursing students. It enables them to expand the knowledge and enhance the quality of their practice. Nursing students must keep abreast of new information, current and emerging trends, medical technology and related scientific and professional publications to be able to function effectively in a constantly changing workplace. Self - directed learning helps nursing students remain flexible, open to change, current in practice skills, and at the same time it helps in the growth of the students’ confidence and professionalism. Objectives : The aim of the study is to evaluate the students' self - directed learning laboratory practices related to administration of medications procedures (Intramuscular injection, Intravenous injection and oral medication ). Methodology : Across sectional design was has been carried out at three colleges of nursing in the south of Iraq and used in the present study from 23th February 2015 to 15th May , 2015. To evaluate nursing students' self - directed learning in applying laboratory practices related to administration of medication in southern of Iraq. The present study was conducted in southern Iraq at three nursing colleges which included (Missan, Thi - Qar and Basra nursing college ). Purposive sample was selected which consist of (90) first year of nursing students( 45) male and (45) female and the sample contained (30) students from each college after the consent of the participants. The check - list consists of two parts, the first part is general information about the study sample of nursing students (4 items) and the second part is an ideal observation check list which includes (73) items including three procedure technique which consists of the intramuscular injection ( 25 )items, intravenous injection (28) items and oral medications (20) items that concerned with students self - directed learning laboratory practices technique. Each procedure has three steps of strategy technique (preparation , equipments and procedure ) which are considered important steps that must be performed by students. The items concerning students self - directed learning laboratory practices are rated on three levels likert scale; (satisfy , need more practice and unsatisfied ). Reliability of the evaluation check - list competency is determined through the use of check - list is based on Cronbach's practices. The instrument validity is determined through a panel of (15) experts.The present study are analyzed through the application of two statistical approaches. A descriptive statistical approach includes (frequency, percentage, Mean of score (M.S.) and standard deviation (SD) and an Inferential statistical approach includes (Chi - Square,t - test, ANOVA). Results : The result of the study indicate that the majority of the first nursing students' satisfaction in general have high level of the self - directed learning in laboratory practices. During application the result show that s (96.7%) intravenous , (93.3%) intramuscular and (88.9%) oral medications administration and also the study declared non significant relationship between self - directed - learning (students lab practice) and their demographic data. Recommendations : This study recommended that : . 1 - Introduce a policy and supportive plans to assist and encourage personnel of every nursing students to the colleges.2 - Extensively apply self - directed learning as a method of learning for their professional and self - development and develop visual aids for learning and set up comprehensive.3 - Update the sources of learning for example public library , the internet service, communications room and/or educational technology room. 4 - Facilitate suitable environment for nursing students in searching for information they need for learning and self - development. 5 - Every these recommendation addressed to ministry of higher education/ colleges of nursing.

تقييم رضا المسنين باتجاه الرعاية الصحية في دار المسنين في مدينة بغداد == Assessment of Elderly'S Satisfaction Toward Health Care At Geriatric Home In Baghdad City

Author name: عادل عبد الزهرة عطية
Supervisor name: محمد فاضل خليفة
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: اجريت دراسة وصفية لتقييم رضا المسنين باتجاه الرعاية الصحية في دار المسنين في مدينة بغداد للفترة من 26 تشرين الثاني 2013 الى3 ايلول 2014، تهدف الدراسة الى تقييم رضا المسنين باتجاه الرعاية الصحية من خلال مجالات الرعاية مثل (طلب الرعاية الصحية، تناول الع | A descriptive study concerning the assessment of elderly's satisfaction toward health care at geriatric home in Baghdad city. From November 26th, 2013 to September 3rd, 2014. is to assess the elderly's satisfaction toward health care through the use of the domains of health care seeking, nutrient intake, physical activity, drug use, psychological aspect, social aspect, and to find out the relationship between elderly's satisfaction and their demographic characteristics of age, gender, marital status, level of education, health state, and economic status. The selection of the sample through a non - probability 'purposive sample' is (N=86) from elderly aged 65 years and over who reside in geriatric home. Data are collected through interview using questionnaire tool and analyzed through the application of descriptive and inferential statistical analysis which reveal, the reliability of the questionnaire which is determined through a pilot study and the validity is achieved through a panel of (18) experts. The study results showed demographic characteristics analysis of the sample concerned, the large number of the sample that was with age (65 - 69) years (34.9%) and also showed that majority of study sample (59.3%) are men and the remaining are women, and with regard to marital status it was found that many of them are widows and their percentage (38.4%) Regarding subject level of education, the greater number of them are illiterate and they are accounted for (48.8%) In addition, the majority of the elderly who suffer from chronic disease are (73.3) while the majority of them have no previous surgery and their percentage (58.1%) Regarding housing ownership (74.4%) they are absent or (do not have a house), and most of the elderly's monthly income is (53.5%) insufficient. The present study concluds : the vast majority of the elderly population are illiterate who do not have opportunity for better education. In addition, the elderly were generally satisfied with health care, and it was the best domain of elderly satisfaction is the nutrient intake and the less domain of health care seeking. While the study showed that, the demographic characteristics of elderly had no impact upon their overall satisfaction toward health care except the level of education. Therefore, that gender affects the satisfaction of the elderly in seeking of health care. In addition, the monthly income effects on elderly satisfaction with nutrient intake. As well as the age and suffering from chronic diseases affecting the physical activity domain and with level of elderly satisfaction in this aspect there is an impact of each gender, level of education and monthly income of the elderly on their satisfaction towards the social domain, the age affects the elderly satisfaction with psychological domain. The study recommended Promotion of health care provided to the elderly especially with regard to access to health care and the practice of physical activity. The Ministry of Labor and Social Affairs, in cooperation with the Ministry of Health can establish a special health center for the elderly to help them to get health care. As well as the establishment of a sports hall equipped to encourage the elderly to exercise of daily activities sports. Work of collective entertainment trips continuously to improve elderly mental and social state. Study should be conduct to assess satisfaction among the elderly residents in geriatric homes and others in their own about health care services (comparative study).

العلاقة بين اصناف الدم المختلفة وعوامل الخطورة الرئيسية لامراض الشرايين التاجية == The Relationship Between Blood Groups And Major Cardiovascular Risk Factors of Coronary Artery Diseases

Author name: رواء هادي شريف
Supervisor name: سامي رحيم الكاتب | احمد نعمة رجيب
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: امراض الشرايين التاجية السبب الرئيسي لمعظم حالات العجز والوفاة في الدول المتقدمة. العمر, الجنس, السمنة, اختلال مستويات الدهون, التدخين, ارتفاع ضغط الدم, مرض السكر, الالتهابات, والاكسدة الضارة من اهم عوامل الخطورة الرئيسية للمرض. مجموعة من الدراسات اوحت ب | Coronary artery diseases are the most important causes of mortality and morbidity in many developing countries. Age, gender, obesity , lipid disturbance, smoking, hypertension, diabetes mellitus, inflammations and oxidative stress are considered as major risk factors of disease. Many of studies suggest that there is association between blood groups and risk factors of coronary artery diseases.Objective : The aims of study are to find if there is an association between blood groups and CADs and to study some of the causes that may be lead to this association. Also, it aims to determine the type of relation between some important factors such as, C - reactive protein (CRP), advanced glycation end products(AGEPs), and glutathione peroxidase - 3 (GPX - 3) with blood groups and coronary artery diseases. Materials & Methods : Ninety persons included in the study (60 as patients and 30 as healthy controls). Where the patients suffering from CAD, the controls are free from this diseases. Their ages ranged between 25 - 70 years old and they were divided into four groups according to the blood groups (A, B, AB, and O). This study is based on case control study done in Al - Sadder Teaching hospital (Cardiac Center) in Al - Najaf Al - Ashraf city from December 2012 - to October 2013. Some information were taken from each person in the study like, name, age, sex, address, weight and height. Finally and after getting permissions, the blood group was analyzed for each person in the study, then the sera were collected from those 90 persons and analyzed by using enzyme linked immunosorbent assay (ELISA) for CRP, AGEPs, and GPX - 3, meanwhile for lipids assay we used spectrophotometer. Results : The results of the study show that the blood group O( 33.7%) predominates in both studied groups (Patients and controls) followed by A (29.6%), B (27.4%), and AB (9%). There is no significant differences in the percentage of blood groups between patients and controls groups (P >0.05). Also, the results of the study show that the incidence of disease increase with age increases and in male (80%) more than female (20%). Also, the results show that only patients group has obese individuals and most obese were from blood group B(80%), followed by A(66.6%), O(60.8%), and AB(50%). The statistical analysis of the study shows that blood group AB among patients and blood group B among controls have a significant increasing (P<0.05) in the serum level of total cholesterol, and C - reactive protein (CRP). While, the results show that blood groups (B&AB) of patients have a significant increasing in the serum level of triglycerides. The blood group A among patients and blood group B among control show a significant increasing in the LDL cholesterol serum level, while blood groups (A& B) of patients have a significant reduction in the serum level of HDL cholesterol. Also, the study shows that blood group A of both patients and controls has a significant increasing in the serum level of advanced glycation end products (AGEPs), while the blood groups (A& B) of patients have a significant decreasing in the serum level of glutathione peroxidase - 3 (GPX3). Finally, the results of the study show a negative correlation (R= - ) between AGEPs and GPX3 in blood groups ( B&O) of both patients and controls group.Conclusions : Results of the present study show that the blood group O predominates in the studied groups in Al - Najaf Al - Ashraf city followed by A, B, and the last is AB. The incidence of CAD 2 to 4 time more common in male than female and incidence of CAD increase with age increases in both sex. The incidence of CAD 2 to 4 time more common in male than female and incidence of CAD increase with age increases in both sex. Also, There is a high percentage of subjects from blood groups (A, B, and AB) suffering from disturbance in the serum level of lipids and high level of CRP, which they were associated with atherosclerosis disease that was considered one of the most important leading cause of CAD.

توازن الحركيات الخلوية وعلاقة اليللات التطابق النسجي في كل من النوع الاول لمرضى السكري واشقائهم في دراسة مقارنة == Cytokine Balance (Th1/Th2) And Hla - Dr Association In Type I Diabetic Patients And Their Siblings In A Comparative Study A Thesis

Author name: محمد علي سلمان علیوي الكعبي
Supervisor name: كریم ثامر مشكور الكعبي | جعفر كاظم الموسوي
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
First pages:
Abstract: It has been proposed that the balance of Th1 and Th2 cells is critical in the development of Type 1 diabetes and much of the genetic susceptibility to IDDM maps to the MHC class II region, thus one approach to investigate the immunopathogenesis of diabetes is to study first degree relatives of affected individuals.A case - control study was performed on 120 persons, they divided into three group which are diabetic, siblings and control (40 persons in each group), who attended to Al Zahraa Teaching Hospital /Diabetic Center in Al Kut between the period from April; 2012 till April; 2013. OGTT performed on both diabetic siblings and control, results have showed that the means of OGTT is highly Significant (P value = 0.000) in both siblings and control groups, whereas there were no Significant differences in the Means of differences of OGTT in both tested groups (Siblings and Control), P value = 0.288, although the mean of differences of OGTT is higher in siblings group (25.3250) than that in control group (22.0750). Cytokines levels (IFN - gamma, IL - 2, IL - 4 and IL - 10) were determined among the study groups (type 1 diabetes, siblings and control) by ELISA method, results showed that the means of IFN - gamma and IL - 2 levels were highly Significant (P value = 0.012, 0.037 respectively) in type 1 diabetic group , in comparison to their corresponds among both siblings and control groups, whereas the mean of IL - 4 levels was non - significant (P value = 0.738) among the study groups (type 1 diabetes, siblings and control), in spite it was higher in control group (0.81936) than that in both type 1 diabetic patients and their sibling (0.74387, 0.77593 respectively).While in case of IL - 10 results showed that the mean was highly significant among control group (P value = 0.030) in comparison to its corresponds among both type 1 diabetic patient and their siblings. Distribution of HLA - DRB1 loci among the study groups (type 1 diabetic patients, siblings and control group) were performed using MR.SPOT ROBOTING system, results showed that the frequencies of HLA - DRB1*3,*4 {25 (69.4%)} among diabetic group were Significant in differences (P value = 0.002), in comparison to the corresponding frequencies among control group {11 (30.6%)}, in comparison to the frequencies of other HLA - DRB1 loci in both diabetic group and control group { 15 (34.1%), 29 (65.9%)} respectively.Meanwhile results showed that there were no Significant differences (P value = 0.116), in the frequencies of HLA - DRB1*3,*4 {25 (58.1%)} among diabetic group in comparison to the frequencies of HLA - DRB1*3,*4 {18 (41.9%)} among siblings group, in compare to the frequencies of other HLA - DRB1 loci among diabetic group and sibling group {15 (40.5%), 22 (59.5%)} respectively, also results have showed that there were no Significant (P value = 0.104), in the frequencies of HLA - DRB1*3,*4 {18 (62.1%)} among sibling group in compare to the frequencies of HLA - DRB1*3,*4 {11 (37.9%)} among control group, in compare to the frequencies of other HLA - DRB1 loci among sibling group and control group {22 (43.1%), 29 (56.9%)} respectively. In another part of this study we estimate the relation between means of HbA1c and their corresponding HLADRB1 Loci among the study groups, result have showed that there was a significant relation (P value = 0.010) between the Means of HbA1c and their corresponding HLA - DRB1*03 Locus among the study groups. In conclusion the higher Mean of differences of OGTT among Siblings group might reflect the potential of them to develop diabetes, also T1D is Th1 mediated disorder, while Th2 cytokines profile might play a protective role against the development of T1D,in addition the genetic predisposition of diabetic Siblings for development of diabetes since, both diabetic group and sibling group showing the highest frequencies of HLA - DRB1 *3,*4, in compare to Control group and finally HbA1c might have an inheriting pattern. In conclusion diabetic siblings more prone for development of diabetes in future.

تقييم حالة الحديد، فيتامين ج والهرمونات في انخفاض الخصوبة لدى النساء == Assessment of Iron Status, Vitamin C And Hormones In Subfertile Women

Author name: ميسم محمد حسين الكعبي
Supervisor name: سامي رحيم الكاتب
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: انخفاض الخصوبة مشكلة شائعة ولكن علاجها في بعض الاحيان غير كافي لعدم تشخيص الاسباب بالكامل. الحديد عنصر ناقل للاوكسجين ولذلك فهو محفز لحيوية كل الخلايا والانسجة في الجسم. نقص الحديد من اكثر مشاكل نقص الغذاء الشائعة في العالم. لذلك ربط الحديد مع الخصوبة يح | Subfertility is a common problem, but the treatment is sometimes inadequate because the causes are not fully diagnosed. Iron is essential for oxygen transport and therefore promotes health of all cells and tissues. Iron deficiency is the most common nutritional deficiency in the world. Therefore, the connection between iron and fertility need more explanation. Some researchers have suggested that iron may be important for ovulation as the egg has an increased demand for iron while it matures and women who do not get sufficient amounts of iron may suffer anovulation and possibly poor egg health, which can inhibit pregnancy at a rate 60% higher than those with sufficient iron stores in their blood. However women planning to become pregnant should test for serum ferritin level and accordingly if low they can use iron supplements because they may help them to prevent iron deficiency and also improve fertility. Vitamin C is essential for the uptake and absorption of iron. The objective of this study was to assess the iron status, vitamin C and some hormone levels in subfertile women. The sample of study consists of 106 women subdivided into two groups : first consist of 76 subfertile women were taken from the outpatient to fertility center in the gynecological and obstetrical teaching hospital in Kerbala city and from multiple gynecology/obstetrics privet clinics. Second consist of 30 fertile women (control and comparative) who obtained from outpatients and some relatives. The subfertile women subdivided into two subgroups according to the cause of subfertility in presented study : first group : ovarian dysfunction (OD) either due to polycystic ovary syndrom (PCOS) or due to other cause (OC) like anovulation, hyperprolactenemia, premature ovarian failure. Second group : unexplained (idiopathic). Also the subfertile women subdivided into two subgroups according to the type of subfertility : primary and secondary. All of them were at reproductive age (16 - 45) years and there BMI ranged between (20 - 45 kg/m2). The study was achieved throughout a period which extends from July 2012 - October 2013. The protocol of study summarized as following : At day 3±1of menstrual cycle (menstrual phase), all women underwent the measurement of concentrations for multiple hormones in the blood like luteinizing hormone, follicle stimulating hormone, estradiole, prolactine and Testosterone. In addition to iron status (serum iron, serum ferrtin and total iron binding capacity) and vitamin C also assessed. And at day 13 of menstrual cycle (near time of ovulation), all women underwent two investigations : first : the measurement of concentrations of luteinizing hormone, serum Leptin and antioxidants as vitamin C and glutathione. In addition to Iron status (serum iron, serum ferrtin and total iron binding capacity) and hemoglobin concentration also estimated. Second : estimation the size and the number of dominant follicles in both ovaries by ultrasonograghy. While at day 23±1 of menstrual cycle (secretary phase), all women underwent two investigations : first : estimation the concentration of serum progesterone to detect ovulation. Second : estimation the endometrial thickness by ultrasonograghy. The results of study shows : • A significant increase (P<0.05) in number of subfertile women with age group (16 - 25) years and there were highly significant decreases (P<0.001) in number of women with age 36 - 45 years for both primary and secondary subfertility. While no significant difference (p>0.05) in age group (26 - 35) years.• A significant increases (P<0.05) in body mass index for subfertile women due to polycystic ovary syndrome. Also a significant increase (P<0.05) in serum leptin for women with overweight and obese as compared with normal weight.• A significant increase (P<0.05) in serum ferritin level for polycystic ovary syndrome at day 13 of menstrual cycle. Also, a significant decreases (P<0.05) in number of polycystic ovary syndrome in those having serum ferritin lower than normal at day 13 of menstrual cycle.• A significant increase (P<0.05) in the number of subfertile women due to ovarian dysfunction other than polycystic ovary syndrome who having a lower serum level of vitamin C at day 3 of menstrual cycle. • A significant increase (P<0.05) in serum testosterone level in polycystic ovary syndrome group at day 3 of menstrual cycle. • A significant increase (P<0.05) in serum prolactine level in group of ovarian dysfunction due to other cause at day 3 of menstrual cycle. when serum ferritin level lower than or within normal value. • A significant decrease (P<0.05) in serum level of luteinizing hormone for group of ovarian dysfunction due to other cause at day 13 of menstrual cycle when serum ferritin less than normal but no significant differences (P>0.05) in serum luteinizing hormone when serum ferritin within normal value. • A significant decrease (P<0.05) in serum luteinizing hormone for group of ovarian dysfunction due to O.C when vitamin C within normal value at day 13 of menstrual cycle.• A highly significant decrease (p<0.001) in endometrial thickness for all subfertile group at day 23 of menstrual cycle.• No significant difference (P>0.05) in serum levels of follicle stimulating hormone, estradiole and glutathione as compared with other parameters in fertile and subfertile women.It was concluded that the decrease in serum ferritin and vitamin C levels in the blood may be associated directly or indirectly with subfertility caused by ovarian dysfunction.

تاثير التلميزارتان والاجسام المضادة لمستقبلات الانجيوتنسين نوع 1 في انقاص الارواء الدموي واعادته لعضلة القلب وموت الخلايا المبرمج في ذكور الفئران == Effects of Telmisartan And Angiotensin II Type1 Receptor Antibody In Myocardial Ischemia/Reperfusion Injury And Apoptosis In Male Mice

Author name: سعاد تريجي زامل العكيلي
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
University: University of Kufa - Faculty Of Medicine - Department Of Medicine And Therapeutics
Language: English
University location: Najaf
First pages:
Abstract: يمثل نقص ارواء عضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. الانجيوتنسين الثاني قد يساهم في الاصابة بسبب اعادة الارواء عن طريق زيادة الاكسدة والعوامل الالتهابية. الانجيوتنسين الثاني يمارس معظم ا | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem following thrombolysis, angioplasty and coronary bypass surgery. Angiotensin II may contribute to reperfusion injury by increasing oxidative stress and inflammatory factors. Ang II exerts most of its effects via AT1Rs. Objective : This study was undertaken to investigate the potential role of Telmisartan and AT1 - AB in amelioration of myocardial I/R injury induced by ligation of coronary artery in mouse model. Materials & method : Adult male Swiss - albino mice were randomized into 6 equal groups. Group (1) sham group : Mice underwent the same anesthetic and surgical procedure as the active control group except ligation of LAD coronary artery.Group( 2) active control group : Mice were subjected to regional ischemia for 30 min by ligation of LAD coronary artery and reperfusion for 2 hours.Group( 3) control vehicle group (1) : Mice in this group injected with DMSO (vehicle for Telmisartan ) via IP route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion for 2 hr. Group( 4) control vehicle group (2) : Mice injected with D.W ( vehicle for AT1 - AB) via IV route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion fore 2 hr. Group (5)Telmisartan treated group : Mice pretreated with Telmisartan 0.5mg/kg i.p 30 minutes before ligation of LAD coronary artery. Group(6) AT 1 - AB treated group : Mice pretreated with AT 1 - AB (1Mcg/gm.) of body weight via IV route 30 minutes before ligation of LAD coronary artery. Results : Compared with the sham group, Levels of TNF - ? & IL - 1?, IL - 6,caspase 3 and plasma level of cardiac troponin I increased in control group (p<0.001).Levels of Bcl2 decreased in control group(p<0.001). Histologically ,All mice in control group showed a significant (p<0.001) cardiac injury. Both Telmisartan and AT1 receptor antibody significantly counteract the increase in myocardium level of TNF - ?, IL - 1B,IL - 6,caspase 3 ,plasma cTnI (P < 0.001). Furthermore, the Telmisartan and AT1 receptor antibody significantly increased in myocardium level of Bcl2. Histological analysis revealed that both Telmisartan and AT1 receptor antibody markedly reduced (P < 0.001) the severity of cardiac injury in the mice underwent LAD ligation procedure. Conclusion : The results of the present study reveal that Telmisartan and AT1 receptor antibody ameliorate myocardial I/R injury in Mice via interfering with inflammatory reactions & apoptosis which induced by I/R injury.

تغيرات الرسم الكهربائي للدماغ عند الحوامل في مرحلة ما قبل التشنج الحملي وعلاقتها ببعض المؤشرات الحيوية الالتهابية == Electroencephalographic Changes In Preeclamptic Females And Their Relation With Some Inflammatory Biomarkers

Author name: مهند يحيى ادريس المحنه
Supervisor name: احسان محمد عبود عجينة
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: يسجل الرسم الكهربائي للدماغ الفعالية الكهربائية الذاتية المتولدة في قشرة الدماغ. اشارة الرسم الكهربائي للدماغ هي قياس للتيارات المتحركة في قشرة الدماغ. عندما تحفز خلايا الدماغ (العصيبات), يتم توليد التيار الارتباطي في التشجرات العصبية للخلايا. يولد هذا ال | Electroencephalography (EEG) records the spontaneous electrical activity generated in the cerebral cortex. When brain cells (neurons) are active, synaptic currents are produced within their dendrites and this current generates an electrical field over the scalp. The EEG waves measure this current that flow during synaptic excitations of pyramidal neurons' dendrites. Preeclampsia is a disease of pregnancy characterized by a blood pressure of 140/90 mmHg or more on two separate occasions after the 20th gestational week in a previously normotensive woman. This is accompanied by significant proteinuria (>300 mg in 24 hours) and is associated with endothelial cell damage. The markers of endothelial activation or inflammation have been blamed to play an active role in preeclampsia (Sibai et al., 2005). This study included 97 pregnant with a confirmed diagnosis of preeclampsia as a patient group with a gestational age ranging between 20 - 38 weeks. They are divided into three subgroups according to their gestational age (20 - 26 wk, 27 - 32 wk and 33 - 38 wk) and the mean of their age was 29.34±4.82, 30.14±5.33 and 32.54±6.22 years respectively. In addition, 72 women with normal pregnancy have been included as control group. These were also subdivided into three subgroups just like that of the patients and their age matches them too. Participants belong to both patient and control groups had underwent same procedure, examinations and tests like EEG and serological tests for Intracellular Adhesion Molecule 1 (ICAM I), interleukin - 6 (IL6) and C - reactive protein (CRP), in parallel. Results showed that there were statistically significant increased levels (P < 0.05) of ICAMI, IL - 6 and CRP values between patient and control groups and between the patient with mild and those with severe preeclampsia in all tested gestational age subgroups. This supports the hypothesis stating a role of cytokines and inflammatory biomarkers in the mechanism underlying preeclampsia. Moreover, and at all gestational age subgroups, the ICAMI, CRP and IL - 6 values were higher in patients with severe disease when compared with those having milkd disease. This strengthens more the relation of these biomarkers with the progression of preeclampsia. There were abnormal EEG changes in about 52 % of the patients with preeclampsia and these changes were reported in all gestational age subgroups and were in the form of spikes, polyspikes, slow sharp waves. This shows that preeclamptic females are prone to some excessive brain activities that may put them in danger of abnormal brain function. Furthermore, these EEG abnormalities were related to the severity of the preeclampsia as they were recorded in severe cases more and at all gestational age subgroups. Also there were statistically significant elevation regarding ICAMI (at the second and third gestational age subgroups), IL - 6 values (at the second gestational age subgroup) and CRP (at all gestational age subgroups) among patients with and without abnormal EEG changes. This suggests a strong relation between these inflammatory biomarkers with the occurrence of abnormal EEG changes in preeclamptic females. In conclusion, an inflammatory process is blamed to be the underlying mechanism of preeclampsia as proved by the elevated levels biomarkers in these patients. Both the abnormally elevated biomarkers and the abnormal EEG changes occur in preeclamptic patient in different gestational ages above 20 weeks and occur in different disease severity.

دراسة مقارنة بين احتياجات التثقيف الصحي وما هو متوفر في مدينة كركوك

Author name: وفاء محمود جاسم
Supervisor name: رقية صبحي توفيق | نشوان نعمت حنا
General topic: Medicine
Specific topic: Community Medicine
Degree: Master
University: Tikrit University - Faculty Of Medicine
Language: English
University location: Salahaddin
First pages:
Abstract: يعد التثقيف الصحي على انه وسيلة اعلامية لجميع الناس لغرض تشجيع وتغيير العادات الى مستوى صحي وصحيح ويمكن ان يعرف التثقيف الصحي بانه مساعدة فئات المجتمع للتخلص من الامراض ووقاية الناس بواسطة تغيير معتقداتهم وافكارهم وسلوكهم نحو الصحة الشخصية تهدف الدراسة ا | Health education is regarded as an informative process achieved for all the people to promote and change their personal habits towards more healthy and accurate , or it may be defined as ( assistance of all community from any disease occurrence with the prevention and protection by affecting their attitudes , believes and behaviors regarding the personal health. This study is an attempt to assess the current situation of primary health centers in Kirkuk city regarding the health educational programs and to assess the main health educational needs and achievements.It is a descriptive study including all the health centers in Kirkuk - district except ( 7 ) of them excluded due to bad security situation in the city and only (12) primary health care centers were conducted starting from the 1st of December / 2007 to the end of February / 2008 , through a questionnaire form which is designed. for follow up the health activities achievement which are adapted by Ministry of Health plan for health directorates and health centers.interviewed by the researcher.The doctors and health educators who were working in these centers were included with main concentration on the educational aspect regarding (teaching aids , records obstacles and educational room with its equipments ). The health centers were classified by health directorate into ( 3) main groups according to the presence of educational methods and equipments and these are : - The first group including (5) model health centers with full , and complete supplementation of educational needs of teaching aids while the second group includes (4 ) health centers with ordinary aids and the last group containing (3) centers with still the old fashion of teaching aids and methods Nearly half of model health centers having (Audio - visual ) methods (45%) but 66.8%) of them were well - prepared updated health educational records And ( 58 %) of the health educators preferring educational models +audio - visual methods and the main educational obstacles are inadequate staff with insufficient methods (85% ) while the best suitable suggestions for avoiding these are supplying these centers with perfect well trained staff and motivation (33%).and (71%) of them agreed with the negative role of health directorate.During the 3 months period before the study , the educational activities for both the health directorate and health centers were affected in December /2007 and became over planned in the other two months (January and February /2008). Lack of the adequate training staff assessing the health educational activities and deficiency in teaching aids and methods necessary for conducting the health programs. with more attention towards motivation aspect with the little time provided for singly health group education and inadequate supplementation of the necessary needs in each health center are the main conclude points in the study

التشخيص المقارن المصلي والجزئي لداء المقوسات وبعض الاصابات الفيروسية لبعض النساء الحوامل اللوات يعانينن من الاجهاض في محافظة النجف الاشرف == Serological And Molecular Comparative Diagnosis of Toxoplasmosis And Certain Types of Viral Infections (Torch) In Aborted Women In Al - Najaf Province

Author name: ذكرى عبد الله محمود المياحي
Supervisor name: باقر عبيس سلطان | صباح نعمة محمد الفتلاوي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة في محافظه النجف الاشرف / العراق، وتضمنت جمع عينات من دم النساء الحوامل اللواتي يعانين من الاجهاض اول مرة او متكررة وكان عدد الحالات 57 حالة. اعمارهن يتراوح بين 15سنة الى40 سنة واللواتي يراجعن مستشفى الزهراء التعليمي والمختبر المركزي وبع | Toxoplasmosis and viral infections such as Rubella , Cytomegalovirus and Herpes virus (TORCH) are feared risk during pregnancy. The rate of toxoplasmosis and of subclinical infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well - known for Rubella , Cytomegalovirus and Herpes virus. The respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presented , also the value of passive prophylaxis, therapy, and prenatal diagnosis as well as the possible management for diminishing the infection problems in pregnancy.Aims of study : The study was aimed to evaluate the ELISA result for Toxoplasma, rubella, cytomegalovirus (CMV) and Herpes virus (TORCH) IgM and IgG and compare the results of the ELISA with RT - PCR to arbitrate discordant results.Methodology : The present study was carried out on 57 pregnant women, all of them had with bad obstetric history (BOH) of abortion twice or more. Patients were between 15 - 40 years of age, and they were attending Al - Zahraa Maternity and pediatric Teaching Hospital, Al - Hakeem Hospital and some private clinics, in AL - Najaf province during the period from May 2012 till May 2013. Serological evaluation for TORCH infections was carried out by using IgM and IgG Enzyme Linked Immunosorbent Assay (ELISA ) method and molecular technique ( DNA; RNA - RT - PCR). Each blood sample was divided into two part , one part plane tube for separation of serum that were screened for the presence IgM and IgG antibodies against Toxoplasma gondii ,Cytomegalovirus Rubella virus and Herpes simplex virus by using (ELISA test ).The tests were performed according to manufactures instruction.The other part (blood )EDTA tube was used for DNA 0f detection Toxoplasma , CMV and Herpes simplex virus , while RNA extraction of Rubella virus.The blood and serum sample were stored in refrigerator (2 - 8C?) before the analysis.The extracted DNA and RNA were stored at - 20C? till used within 24 hours. All ELISA Results samples were compared with Real - Time PCR result. Statistical analysis : Statistical program SPSS version (20.0) was used to study the significant difference between tests in each type of antibody (IgM and IgG). (X2 - test) was used after detection of normal distribution to the data and appropriate P<0.001 consider significant. Results : Both IgM / IgG was Seropositive to T. Gondii , Rubella , CMV and HSV - 2 which were 49.12/80.70%, 21.05/49.12% , 45.61/70.18% and 1.21/36.20% respectively. Samples were seropositive to IgM and results were retested by RT - PCR method. Maximum number of IgM /IgG seropositive cases of aborted women was 19(32.75%)/57 that associated with Toxoplasma ,Rubella and CMV infection by RT - PCR ( 10(17.54%), 6( 10.53%) and 3(5.26%) respectively ). The ELISA TORCH IgM and IgG assays provided results comparable to RT - PCR indicated significant differences among Toxoplasma and viral infection. The ELISA TORCH infection IgM and IgG assays showed a lower specificity with for Toxoplasma IgM (p=0.001), poor for CMV IgM and moderate for Rubella virus IgM.Conclusion : The accurate diagnosis of TORCH infection should be done by molecular method.

افرازات حلمة الثدي كعلامة دالة حول امراض الثدي في مستشفى بعقوبة التعليمي == Nippel Discharge As Presentation of Underlingbreast Diseases At Baqubah Teaching Hospital

Author name: فراس منذر متعب
Supervisor name: مصطفى خليل حميد
General topic: Medicine
Specific topic: Surgery
Degree: Master
University: University of Diyala - Faculty Of Medicine
Language: English
University location: Diyala
First pages:
Abstract: افرازات حلمة الثدي مشكلة عرضية شائعة تسبب الكثير من المضايقة والقلق للنساء, وقد تكون اول ما يشتكي منها المريضة، افرازات حلمة الثدي يمكن ان يكون علامة التقديم الاسبق لامراض الثدي الخبيثة.الغرض من الدراسةهذه الدراسة بنيت على ت

تاثير التعويض الجزئي والكلي للاسنان على فحوصات كفاءة الرئتين عند مرضى السكري نوع - 2 == Effect of Partial And Complete Denture Treatment On Spirometric Tests In Type 2 Diabetic Patients

Author name: حسين مهدي حسين الخفاجي
Supervisor name: يسار محمد حسن الشماع
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: في هذه الدراسة العرضية, تم اختيار 140 شخصا بصورة عشوائية, من بينهم 90 كان مصابا بالسكري والباقون كانوا سليمين ,لدراسة تاثير ارتداء طقم الاسنان الكلي والجزئي من قبل مرضى السكري على فحوصات كفاءة الرئتين وبالتحديد, , السعة الحيوية القسرية (FVC)و الحجم الزفير | In this cross sectional study, a total number of 140 subjects selected randomly, 90 of them were diabetic and the remaining were normal, to study the effect of complete and partial dentures wearing by diabetic patients on pulmonary function test particularly peak expiratory flow rate (PEFR), forced expiratory volume in the first second (FEV1), forced expiratory volume ratio (FEV1/FVC) ratio (FEV1%), forced vital capacity (FVC), and also the retropharyngeal space (RPS), the level of Endothelial selectin (E - selectin) and the level of Vascular Cell Adhesion Molecule - 1(VCAM - 1)was also compared, in this study, between the diabetic and the normal subjects.The results indicate that the spirolab III was accurate and reproducible and can be used in all related studies.The effect of wearing of complete and partial dentures by diabetic patients on FVC, FEV1, PEFR and also the degree of alteration of the RPS were measured.1) The results of the study shows a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), while there was no significant change in the value of FVC after the replacement of complete denture.2) No statistically significant differences in the values of FVC, FEV1, FEV1%, PEFR and retropharyngeal space (RPS) was observed after replacement of partial denture without free end extension3) There was a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), never the less there was no significant change in the value of FVC after the replacement partial denture with unilateral free end extension.4) There was a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), However there was no significant change in the value of FVC after the replacement partial denture with bilateral free end extension5) The results showed that in poor and good control diabetic patients there was a significant decrease in the values of FEV1, PEFR, FEV1%and RPS after the removal of all types of dentures(p<0.05), while no significant change in FVC.6) Statistical significant increase in Serum E - selectin and VCAM - 1 level was found in diabetic patients when compared to that of the normal subjects (p<0.05).7) No statistical significant difference between serum E - selectin and VCAM - 1 level in diabetic patients after and before denture placement (p>0.05). The cause of the significant change in the values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) in case of complete and partial denture with free end extension replacement may be due to pressing of the retropharyngeal space bilaterally and unilaterally by the complete and partial denture and the FVC was not significantly changed because it may not be affected by denture support, while the elevated Serum E - selectin and VCAM - 1 level might indicate endothelial activation and inflammation.In conclusion we can conclude that 1. The spirolabIII used in this study is an accurate and reproducible device and can be used in the assessment of lung function test.2. Values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) where significantly higher after the replacement of complete denture and partial denture with bilateral and unilateral free end extension in diabetic patients (p<0.05), while there was no significant change in the value of FVC.3. No statistically significant differences in the values of FVC, FEV1, FEV1%, PEFR and retropharyngeal space (RPS) was observed after partial denture without free end extension has been replaced in diabetic patients.6. In poor and good control diabetic patients there was no significant change in the value of FVC, while the values of FEV1, PEFR, FEV1%and RPS where significantly decrease after the removal of all types of dentures.8. Statistical significant increase in Serum E - selectin and VCAM - 1 level was found in diabetic patients when compared to that of the normal subjects.9. The results for serum level of E - selectin and VCAM - 1 shows no significant difference diabetic patients after the placement of all types of dentures (p>0.05).

تاثير مستخلص الكركم الخام على التعبير الجيني لمورثة البروتين المنظم للغشاء الحيوي لجرثومة Streptococcus mutans المعزولة من التجويف الفمي == Effect of Curcumine Crude Extract On The Gene Expression of Biofilm Regulatory Protein A (Brp A) In Streptococcus Mutans Isolated From Oral Cavity

Author name: نزار احمد شهاب
Supervisor name: عدنان حمد عبيد الحمداني | عدنان حمد عبيدالحمداني
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Al-Qadisiyah - Faculty Of Medicine
Language: English
University location: Qadisiyah
First pages:
Abstract: جمعت 100 مسحة فموية من مرضى تسوس الاسنان وامراض ما حول الاسنان الذين تراوحت اعمارهم ما بين 15 - 65 سنة ومن كلا الجنسين والذين راجعوا المركز التخصصي لطب الاسنان في الديوانية للمدة من كانون الاول 2012 الى اذار 2013 لغرض قياس التعبير الجيني لمورثة البروتين ا | A total of 100 oral swabs were collected from patients suffering from dental caries and priodontal infections, ranging from 15 - 65 years old of both genders whom admitted to the Al - Diwaniyah Teaching Special Center of Dentistry within the period from December 2012 to March 2013, in order to quantify the gene expression of biofim regulatory protein (Brp A) in Streptococcus mutans isolates by using the Real Time polymerase chain reaction. According to morphological, cultural characteristics and biochemical tests by using VITEK - 2 system, 34/84(40.47%) isolates of S.mutans were isolated and identified among streptococcus species and the isolation percentages were 26/50(52%) in males and in females were 24/50(48%), while the age group (15 - 25 years old) was the most affected than the other groups. The statistical analysis showed no significant differences among the interaction between gender and age groups. Molecular detection of 16s rRNA gene was amplified to confirm the diagnosis of S.mutans isolates, where all isolates gave an amplicon size (151) bp, in addition to, the DNA sequencing was done in order to identify the nitrogen bases sequences and phylogeny of local isolates in comparison with the global isolates (Spain, Japan, Korea, USA, Canada, India), results revealed that the local isolates of S.mutans had a similarity percent (90%) with Spain isolates.Furthermore, the susceptibility of isolates to a variety of antibiotics have been investigated. It has been found that isolates had multiresistance to erythromycin(88.2%),ampicillin(76.4%),amoxiclave(70.5%),tetracycline(61.7%), nalidixic acid and amoxicillin (58.8%), chloramphenicol (55.8%). The statistical analysis showed a significant differences among tested antibiotics. Biofilm formation was investigated phenotypically by using tissue culture plate with crystal violet staining for resistant S.mutans isolates, the results revealed that all isolates were high biofilm former, furthermore, the effect of ethanolic extraction (50%) of curcumine in growth inhibition and biofilm reduction of S. mutans was assayed. The results showed an effect of curcumine in comparison with chlorhexidine(2%) as a control.The gene expression of biofilm regulatory protein A(Brp A) was quantified in comparison with housekeeping gene 16s rRNA as a reference gene of biofilm S.mutans by using reverse transcriptase polymerase chain reaction (RT - PCR). The results revealed that the expression (relative&absolute) of Brp A gene is decreased in the test treatment (S.mutans with 1% glucose+ 50% curcumine) in comparison with control (S.mutans grown in 1% glucose only).In conclusion, the present study established the role of Brp A gene in biofilm formation

تعقب الليشمانيا الجلدية بالاختبارات التحليلية الوبائية والطفيلية والجزيئية والكيمياحياتية == Tracking of Cutaneous Leishmaniasis By Epidemiological, Parasitological, Molecular And Biochemical Analysis

Author name: سندس نصیف الحجیمي
Supervisor name: باقر عبیس سلطان | محسن عبد الحسین الظالمي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
First pages:
Abstract: The present study was performed to identify the species and strain of Leishmania parasite isolated from different endemic areas. It was carried out on cutaneous leishmaniasis (CL) in five Iraqi provinces as following : Al - Najaf province ( Al - Hakeem and Al - Sadder teaching hospitals), Babylon province ( Marjan hospital ), Al - Qadisya province (AL - Sadder Teaching hospital ), Karbala province (Al - Hussain General Hospital) and Kut province (Al - Zehraa Teaching and Al - Kerama Hospitals). It covered seven hospitals as a field of investigations from October 2010 to December 2012.Cellulose acetate electrophoresis has been performed in Walter Reed Institute of researches in USA. A total of 126 cases comprising 48(38%) females and 78(62%) males were studied. The highest infected age group was 21 - 30 years with a rate of 27.8% and the lowest rate was 11.9% at the age group of 10 and less years.Geographical distribution of CL among hospital patients indicated that rural areas were with highest rate (54%) while in urban areas were with(46%).The number of ulcers per one patient differs for each patient. The highest rate of multiple lesions was 73% in comparison with single lesion (27%).The type of infection showed that disease was in wet type (82.5%) more than in dry type (17.5%). Direct smears were made by lesion aspiration,skin scraping and discharged blood methods , by which 90 (71%) patients gave positive result under light microscope.Modified NNN medium and RPMI - 1640 with fetal bovine serum followed by sub - culture in drosophila Schniders media were performed for each case. Out of 126 cases,83(65%) gave positive growth. Further characterization of the causative parasite species and strain made by molecular and biochemical techniques.Out of 83 postive growth culture,only 52 specimens were studied by nested - PCR, using kinetoplast minicircle fraction amplification to detecte the species of parasite.It was found that 45(86.5%) cases in the generation of a 560 bp DNA and 7(13.4%)patients displayed a fragment of 750 bp, corresponding to L. major and L. tropica, respectively.This test revealed that L. tropica and L. major are the causative agents of infection with dominant shifting to the L. major.Cellulose acetate electrophoresis (CAE) was performed for 20 mass cultivated cultures. Isoenzyme profiles of these isolates were compared with reference strains of Leishmania spp. using cellulose acetate electrophoresis and 4 enzyme systems (glucose phosphateisomerase,leucil phosphate,manose phosphate isomerase and 6phosphogluconate dehydrogenase). The results showed that L.major(LV39) isolate were in 20 mass cultivated culture.Most of the CL patients in Najaf province(2011,2012) were from Haidariya in north district. High prevalence of disease was observed in Autumn and Winter.The highest number of cases was recorded during February and December.

مقارنة بين النوع الثاني من مرض السكري ومرض السكري المناعي الذاتي المتاخر الظهور للبالغين باستخدام مؤشرات الخلايا اللمفاوية وملف الحركيات الخلوية == Comparison Between Type 2 Diabetes Mellitus And Latent Autoimmune Diabetes of Adults Using Lymphocytes Markers And Cytokines Profile

Author name: سوسن محمد جبار الحسناوي
Supervisor name: سلمان عزيز الجبوري
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
First pages:
Abstract: This study was done to investigate whether Type 2 diabetes and Latent autoimmune diabetes of adult (LADA) patients have similar or different immunological profile.Therefore, measurement of serum levels of adhesion molecules (Serum intracellular cell adhesion molecule(sICAM - 1), Serum vascular cell adhesion molecule (sVCAM - 1) and Serum endothelial Selectin (sESelectin)), chemokines (CCL2, CCL3 and CCL4) and cytokines (IL - 6, IL - 1receptor antagonist and IFN - ?) in these patients was done. In addition to the influence of BMI, gender, age and diabetes duration on these systemic immune mediators were analyzed. Also, the relationships of the in vivo levelsof these mediators with micro - & macro - vascular complications in type 2 diabetes & LADA were studied.Three hundred individual were enrolled in this study and were divided into two subgroups : patients and control groups (280 patients& 20 control). From the 280 patients group who were clinically diagnosed as type 2 D.M., 34 patients were GADA positive and considered as LADA cases. Serum samples were taken from The 34 LADA cases, 36 cases of D.M.2 (selected randomly from the remaining 246 diabetic type 2 patients) & 20 healthy control subjects.The prevalence of LADA among D.M.2 patients was 12.1%. A significant difference in family history, BMI, FBS, HA1C & duration of D.M. was found in D.M.2 cases compared to LADA cases. The mean sVCAM - 1level of control group was significantly lower than D.M.2 and LADA group, P<0.05. The sE - Selectin level was higher in LADA group than control and D.M. type 2 group & significantly different when compared to control group (P<0.05). The sVCAM - 1 level had a significant positive correlation (R=0.25, P=0.017) with BMI in all groups. LADA group had significantly higher values of CCL2 while D.M. type 2 had the least values (P=0.002). The mean IL - 6 of D.M. type 2 group was (11.6±3.1), for LADA group it was (12.4±1.7) and for the controls it was 0.95 ± 0.14. There was a statically significant difference between D.M.2 vs. controls, and LADA vs. controls (P<0.05), regarding the mean IFN - ?, it was significantly higher in LADA group than D.M.2 group (87.5 ± 20.3) vs. (40.3 ± 4.5) respectively, (P=0.032).The mean IL - 1receptor antagonist was significantly higher in D.M. type 2 and LADA cases than controls (P<0.05). A significant positivecorrelation between IL - 6 and sICAM - 1, (R= 0.27, P= 0.013) & a highly significant positive correlation between IL - 6 and IL - 1RA, (R= 0.35, P=0.001) were found. IL - 6 & IL - 1RA serum levels in D.M. type 2 patientswere significantly associated with the complications where p - value?0.05. For conclusions, LADA is clinically underestimated among D.M. type 2 patients and increases with increasing age. Also, Levels of CCL2 and IFN - ? are associated with autoimmunity, Levels of adhesion molecules & chemokines are associated with complications of diabetes.

التقييم التجريبي لتاثير الروزفستاتين المضاد للالم والمضاد للالتهاب وتداخله مع السليكوكسب والباراسيتامول == Experimental Evaluation of The Antinociceptive And Anti - Inflammatory Effects of Rosuvastatin And Its Interaction With Celecoxib And Paracetamol

Author name: سرمد عبد العباس كشمر
Supervisor name: عبد الله محمد جواد
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
University: University Of Basrah - Faculty Of Medicine - Department Of Medicine
Language: English
University location: Basrah
First pages:
Abstract: اظهرت الدراسات بان الستاتينات تؤدي الى تقليل الوفيات بدرجة اكبر من ان تعزى الى تاثيرها الخافض للكوليستيرول بمفرده لان الفائدة حدثت بشكل اسرع مما يمكن تفسيره على وفق الالية سالفة الذكر. هذه الفوائد يمكن ان يكون لها علاقة بتاثيرات الستاتينات المضادة للالتها | Studies revealed that statins can result in a larger mortality benefits than can be readily explained by their cholesterol - lowering effect alone since they occur too quickly to be explained by the above cited mechanism. These benefits might be related to the anti - inflammatory and other effects statins may have.AimTo find out the extent to which rosuvastatin (a hydrophilic statin) can be considered as an antinociceptive and anti - inflammatory drug in comparison to two standard drugs; paracetamol and celecoxib, and whether its potential antinociceptive effect differs in different pain models. The interaction of rosuvastatin with paracetamol and celecoxib will also be investigated. MethodsMice (a total of 132) of either sex, 3 - 4 weeks of age, 20 - 25 gm body weight, were used (22 mice for each of six groups). Tests for nociception : tail flick, hot plate and formalin tests; and for inflammation (formalin for chronic inflammation, carrageenan - induced paw edema, and TNF - alpha level in blood) were used. Rosuvastatin (7mg/kg), paracetamol (40mg/kg), celecoxib (6mg/kg) or their combination were administered orally once daily in a volume of 0.2 ml. TNF alpha level in blood was measured using ELISA kit.ResultsThe antinociceptive effect of rosuvastatin when investigated in mice using tail flick, hot plate and formalin tests, showed that rosuvastatin has a mild antinociceptive effect which is much less than that of paracetamol and celecoxib tested in the same pain models. It increased the latency for tail flick by only 13.3% when compared to pre - treatment measurements, and in formalin test, it reduced the licking time by 20.9% in comparison to control. The administration of rosuvastatin with either paracetamol or celecoxib did not add to the antinociceptive effects of the latter two drugs (except in formalin test of pain model). None of the above mentioned drugs significantly reduced hind - paw edema when measured 24 hours after formalin injection, while they produced a significant edema - reducing effect after 14 days. Rosuvastatin and paracetamol had nearly similar effect (54.12% and 58.37% reduction compared with control). Celecoxib reduced the hind - paw edema by 73%. Again there was no additive effect between rosuvastatin and either paracetamol or celecoxib; in contrast, rosuvastatin reduced nearly all the effects of celecoxib when given in combination. Similar trend was found when edema was induced by carrageenan injection. TNF alpha level in blood had been reduced by all the three drugs and their combinations but did not reach statistical significance except in the group of rosuvastatin and paracetamol combination.ConclusionRosuvastatin showed a significant antinociceptive effect in tail flick and in formalin test, but not in hot plate test. It had anti - inflammatory and edema - reducing effects in models of inflammation in mice but the effect was less than that of celecoxib and even paracetamol. These rosuvastatin effects did not add to those of paracetamol and had caused a reduction in celecoxib (except for formalin pain model) effects when given in combination.

تاثير الايتانرسبت على السايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الفاران == Effect of Etanercept Against Myocardial Ischemia/Reperfusion Injury In Male Mice

Author name: سيف محمد حسن
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
University: University of Kufa - Faculty Of Medicine - Department Of Medicine And Therapeutics
Language: English
University location: Najaf
First pages:
Abstract: يمثل نقص ارواءعضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. تشمل اصابة عضلة القلب بسبب نقص التروية الدمويه واعادتها ضعف مقلص القلب، عدم انتظام ضربات القلب وكذلك تلف الخلايا العضلية التي لا رجعة في | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia - reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. These changes are considered to be the consequence of imbalance between the formation of oxidants and the availability of endogenous antioxidants in the heart. Objective : This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model.Material & method : adult male Albino mice were randomized into four equal groups.1. Group (1) : Sham group : mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery.2. Group (2) : Control group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours.3. Group (3) : Control vehicle group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice received vehicle of etanercept (normal saline) 5 minutes before reperfusion via I.P injection and.4. Group( 4) : Etanercept treated group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice treated with etanercept 5mg/kg i.p 5 minutes before reperfusion XVIIIResults : Compared with the sham group, the levels of TNF - ? & IL - 1?, IL - 6, Caspase 3 and plasma level of cardiac troponin I increased in the control group but decreased level of Bcl - 2 (p<0.01).Histologically, all mice in the control group showed significant (p<0.01) cardiac injury and apoptosis.Etanercept significantly decreased in myocardium level of TNF - ?, IL - 1B, IL - 6,Caspase 3 , and plasma cTnI (P < 0.01), while significantly increased level of Bcl - 2 (P < 0.01). Histological analysis revealed that etanercept markedly reduced (P < 0.01) the severity of cardiac injury in the mice underwent LAD ligation procedure. : ionsConcluThe results of the present study reveal that etanercept may ameliorate myocardial I/R injury in mice via interfering with inflammatory reactions and apoptosis. : RecommendationAfter studying the results of the present study, the following recommendation to further1. Further measuring the P - selctine and E - selectine to show the effect of etanercept on rolling of eutrophils and platelets that cause further occlusion of blood vessels.2. Further measure the adiponectine (that have a cardioprotective effect ).

العلاقة بين اعتلال الجهاز العصبي اللاارادي الناتج عن السكري مع مستوى اللبتين وامراض القلب حسب فحص ايكو القلب وفحص اجهاد القلب == Relation of Diabetic Autonomic Neuropathy With Leptin And Cardiac Disease Assessed By Echo And Treadmill Test

Author name: زينب فلاح حسن
Supervisor name: احسان محمد عبود عجينة | علاء حسين عباس
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Najaf
First pages:
Abstract: الخلفية : الاعتلال العصبي اللاارادي القلبي له تاثير سلبي على الحياة ونوعيتها للاشخاص المصابين بالسكري. الاعتلال العصبي اللاارادي القلبي يعتبر نوع فرعي من الاعتلال العصبي السكري. هذا النوع من الاعتلال هو الاكثر بروزا لتهديده للحياة وبسبب توفر الفحص القلبي | Background : Cardiac autonomic neuropathy (CAN) has a negative effect on survival and quality of life in people with diabetes mellitus (DM) and is regarded as a subtype of the diabetic autonomic neuropathy. This type of neuropathy is the most prominent focusing because of its life threatening and the availability of the cardiovascular tests that can diagnose it. It has been thought that many factors in DM like leptin causing increase the risk of cardiovascular disease. Researches about the relation of leptin with CAN and cardiovascular disease are yet limited.Aim of study : This study aims to determine the prevalence of CAN in patients with type 2 diabetes mellitus, evaluate the association between CAN the presence of left ventricular dysfunction in these patients, test the association between CAN and ischemic heart disease and assess the relationship between the presence and severity of CAN with fasting serum leptin in these patients.Patients, materials and methods : This cross sectional study included 103 (56 males and 47 females) diabetic patients without hypertension or pre - diagnosis of ischemia. They had attended to the diabetic center in Marjan Medical City in Hilla from March 2013 to February 2014. The patients had undergone thorough assessments that included clinical (history and full examination) and biochemical assessments (measurement of fasting plasma sugar, glycated haemoglobin, lipid profile, blood urea, serum creatinine and serum leptin level). Cardiac autonomic reflex tests were also assessed along with echocardiography and treadmill test. Cardiac autonomic reflex tests included the five tests of Ewing protocol. Patients who had two or more abnormal tests were considered as having CAN (75 patients) and those with less than two abnormal tests were considered as patients without CAN (28 patients). Results : The study found that most diabetic patients (84%) were obese (BMI?25 kg/m2) and most of the diabetic patients presented with CAN (72.8%). Fasting serum leptin level was higher in female than in male in both study groups (with and without CAN). Patients with CAN were older when compared to patients without CAN (p?0.01) and had longer duration of DM (p?0.01) with significantly higher fasting serum leptin levels. The leptin level showed a tendency to increase as the score of CAN increased. However, these changes were not significant (P>0.05). The most abnormal sympathetic response was diastolic blood pressure response to hand grip while the most abnormal parasympathetic response was heart rate response to breathing. On contrary, the lowest abnormal response was found in postural blood pressure test. Echocardiography showed that most of the diabetic patients (89.8%) had a left ventricular hypertrophy by measuring the left ventricular mass index. However, there were no significant changes in echocardiographic results between patients with CAN and those without.Conclusions : This study concludes that CAN is a common complication of type 2 diabetes that affected a large percentage of diabetic patients. The duration of the diabetes and the age of patients are important non modifiable risk factors for the development of CAN. Additionally, heart rate variability is considered as an important test for early detection of CAN. On the other hand, CAN has no impact on the occurrence of left ventricular diastolic dysfunction. Leptin level was high in diabetic patients and even higher in those with CAN and its levels related positively with the severity of CAN. Also, there were high leptin levels in diabetic patients with positive treadmill test results, although these changes were not significant (P>0.05).

فاعليه تطبيق الممرضين للممارسات القياسية على العناية بالاطفال حديثي الولادة المصابين بمتلازمة كرب التنفس في وحده العناية بالاطفال حديثي الولادة في مستشفيات بغداد للاطفال التعليمية == Effectiveness of Nurses Application of Standard Practices On Care of Newborn With Respiratory Distress Syndrome In Neonatal Care Unit At Children And Maternity Teaching Hospitals

Author name: محمد عزيز حسن
Supervisor name: اقبال غانم علي معلة
General topic: Medicine
Specific topic: Nursing
Degree: Doctorate
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: اجريت دراسة شبه تجريبية في مستشفيات بغداد للاطفال التعليمية للفترة من 28 من كانون الثاني الى الاول من ايلول لسنة 2014 لتقييم فاعلية تطبيق الممارسات القياسية للممرضين والذين يتعاملون مع الاطفال حديثي الولادة المصابين بمتلازمة كرب التنفستم تقييم الحاجة من | A quasi experimental(interventional) study was carried out at Baghdad pediatric teaching hospitals in Baghdad from the 28th of January to the 1st of September 2014 to assess the effectiveness of the application of standardized practices to nurses dealing with neonate with respiratory distress syndrome. Assessment of needs was done to evaluate the nurses practices towards neonates with respiratory distress syndrome in neonatal care unit(NCU) by using a special format which includes multiples questions related to nurses knowledge and practices for 10 nurses works at the private Nursing home.The result of the assessment revealed that the nurses have poor knowledge and practices towards neonates with RDS. A pretest test was performed before the application of the program for 35 nurses, three of them was out of the study sample.Observation their practices based on the standardized items which was prepared by the researcher and extensive review of the literature. A non - probability (purposive) sample of 32 nurses who work in the neonate intensive wards was chosen to be involved in study sample, and an educational program was designed based on the results and basic nursing guidelines related to care for neonate with respiratory distress syndrome, it consists of two parts : the first part consists of demographic data, and the second part consists of an observational checklist format related to the nurses practices in care of neonate with respiratory distress syndrome. The questionnaire was used to observe the nurses practices in the pre - test applied before the program started , post - test 1 immediately after the application of the program, post test 2 after 1 month from the post test 1,and a post 3 after 2 months from the second post test. The reliability of the questionnaire was conducted through a pilot study and validity through a panel of experts. The data were analyzed through the application of a descriptive statistics frequencies ,percentages ,and application of inferential statistical procedures which include Pearson correlation coefficient and paired test. The finding of the study revealed that the nurses practices towards neonates with respiratory distress syndrome were improved after the implementation of the program in most of the items of the post test 1 ,2,and 3 of the program, and there is a significant association between nurses' practices and their level of education in most of the program items, There was also a significant association between nurses practices and years of their experiences. The study recommended increasing knowledge and practices of the nurses working in the neonatal care unit through regular training courses, in addition to increasing the number of graduated nurses from the College of Nursing in this critical unit.

تاثير البرنامج التثقيفي التمريضي على معارف وممارسات الملاك التمريضي ازاء التاهيل القلبي لمرضى النوبة القلبية == The Effectiveness of Nursing Educational Program On Nurses Knowledge And Practices Toward Cardiac Rehabilitation For Patients With Heart Attack

Author name: احمد عجلان علاوي ناصر
Supervisor name: حكيمة شاكر حسن
General topic: Medicine
Specific topic: Nursing
Degree: Doctorate
University: University of Baghdad - Faculty Of Nursing
Language: English
University location: Baghdad
First pages:
Abstract: اجريت دراسة شبه تجريبية في مستشفى الشيخ زايد ومستشفى الصدر العام ومستشفى الكرخ العام ومستشفى الكرامة ومدينة الامامين الكاظمين الطبية ومستشفى بغداد التعليمي للفترة من الاول من ايلول2013 الى الاول من ايلول2014 تهدف الدراسة الى تقويم فاعلية البرنامج ال | A quasi - experimental design study was carried out at the AL - Sheikh Zayed hospital; AL - Sader; Al - Karkh ;AL - Karama ;Imameinkadhimein medical city and Baghdad Teaching hospital during the period from 1st September 2013 to 1st September 2014. The objective of the present study is to evaluate the effectiveness of nursing education program on the nurses knowledge and practice toward cardiac rehabilitation phase one for patients with heart attackThe program and instrument were constructed and developed by the researcher for the purpose of the study A random sample composed of (80) nurses was divided into two groups , the experimental group consisted of (40) nurses exposed to the nursing educational program and control group consisted of (40) nurses were not exposed to the program.Reliability of instrument was determined through the use of test and retest for the knowledge test and inter - observation for practice and the instrument validity was determined through a panel of experts. The instrument to measure the effectiveness of nursing educational program on nurses knowledge and practice included (30) items concerning the nurses knowledge and 58 items included nurses practice nurses practices toward cardiac rehabilitation phase one for patients with heart attack.The analysis of data was performed through the application of description statistic(Frequencies, Percents, and Cumulative Percentages , Mean of Score , Standard Deviation , Relative Sufficiency ) and Inferential statistical (Contingency Coefficients , Chi - Square test , Fisher to present the differences between the experimental and control groups. The results of the study shows that the nursing educational program regarding nurses knowledge toward cardiac rehabilitation program phase one is effective. They also shows that there is a good improvement with highly statistically significant differences between pre - post tests of experimental group in overall domains related to nurses practice in items of health assessment heart, assessment chest pain, cardiogenic shock, daily activity living and health education. The study concluded that the nurses working in coronary care unit have deficient knowledge and practices toward cardiac rehabilitation phase one for patient with heart attack. The study also confirms that the instruction oriented program on nurses in the experimental group is successful. The study recommended that the nurses staff can be encouraged and having motivation for being participated in the special training programs and conference to fulfill the nurses needs concerning defects and limitations ed in their practice toward cardiac rehabilitation phase one for patient with heart attack.

المتغيرات الكيميائية الحيوية في مرضى فقر الدم البحر المتوسط نوع بيتا كبرى == Biochemical Changes In Beta - Thalassemia Major Patients

Author name: مضر خضر محمد
Supervisor name: طارق حفظي الخياط | احمد شمران الوطيفي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine
Language: English
University location: Babylon
First pages:
Abstract: امراض فقر دم البحر المتوسط هي مجموعة من امراض فقر الدم الناتجة من مشاكل وراثية في انتاج الصبغة الحمراء (الهيموغلوبين) . هذه الامراض هي من بين اغلب الامراض الشائعة نتيجة الخلل الوراثي في العالم . يحدث النوع (بيتا) منه بسبب القلة في بناء الوحدة البروتينية المسمى (بيتا - غلوبين) من الهيموغلوبين نوع (ا) . الاشخاص الذين يورثون المورثتين صاحبات الخلل ينتج عنه عدم انتاج مطلق لهذه الوحدة البروتينية اعلاه مثل النوع فقر الدم البحر المتوسط نوع (بيتا)كبرى وهذا الخلل يؤدي الى زيادة في انتاج الوحدة البروتينية المسماة (الفا - غلوبين)تم اجراء هذة الدراسة على مرضى مصابين بفقر الدم البحر المتوسط نوع بيتا - كبرى .٦٠ مريض تم اختيارهم لهذه الدراسة كانوا يعانون من : ( فقر الدم , يرقان , تضخم الطحال ) وتم تشخيص المرضى سريريا على اساس وجود فقر الدم الحاد , واستعمال الفصل الكهربائي للهيموغلوبين .الهدف من هذة الدراسة لتحديد التغيرات بصورة الدهون لدى هؤلاء المرضى نتيجة نقل الدم المستمر لهم الامر الذي سيؤدي الى الكثير من المشاكل منها : ترسب الدهون المؤكسدة في الاوعية الدموية , وتحلل الغشاء الخلوي وبالتالي خلل نسيجي وكل هذا يحدث بسب الجهد التاكسدي الناتج من تراكم الحديد وتجاوزه الحد الاعلى في الدم والانسجة (وخصوصا الكبد ).علاج المرضى يتكون من : ١ - نقل الدم الغير منتظم ٢ - زرق منظم الحديد ( ديسفريوكزامين ) وبصورة غير منتظمة ايضا تم اخذ عينات الدم من المرضى ومجموعة الضبط وفصل هذه العينات الى مصل وبلازما وحسب الحاجة وتم قياس التالي : الكولسترول الكلي , الكولسترول في البروتينات الدهنية عالية الكثافة , الكولسترول في البروتينات الدهنية واطئة الكثافة , الدهون الثلاثية , دهون البيروكسيد الكلية , الحديد , الفرتين , النحاس , الزنك , مضادات الاكسدة الكلية , انزيم الالانين ترانس امينيز وانزيم الاسبارتيت ترانس امينيز .مجموعة الضبط تتكون من ٤٠ شخص لهم نفس الاعمار.تم قراءة نتائج المرضى مع مجموعة الضبط ووجد الاتي : هنالك انخفاض معنوي في كل من الكولسترول الكلي , الكولسترول في البروتينات الدهنية عالية الكثافة , الكولسترول في البروتينات واطئة الكثافة , بينما هنالك زيادة معنوية في مستوى الدهون الثلاثية .هنالك زيادة معنوية في مستوى دهون البيروكسيد الكلية , الحديد . والفرتين مع نقصان معنوي في مستوى مضادات الاكسدة الكلية , نقصان معنوي في مستوى الزنك مع زيادة معنوية في مستوى النحاس .النتائج وضحت ان صورة الدهون والمواد الاخرى المقاسة تتغير بصورة معنوية في المرضى عدة عوامل مثل : تراكم الحديد , اصابة الكبد , خلل في الهرمونات يمكن ان يسبب هذه التغيرات.تم استنتاج ان الجهد التاكسدي وقلة مضادات الاكسدة الناتج يلعب الدور المهم في مرض فقر دم البحر المتوسط نوع بيتا - كبرى . | The thalassemia are a group of anemia that result from inherited defects in the production of hemoglobin (Hb) . The thalassemia are among the most common genetic disorders world wide. The biochemical signature of β - thalassemia is reduced synthesis of the β - globin subunit of HbA (α2β2).Individuals inheriting two β - thalassemic alleles experience a profound deficit in β - chain production (homozygous) like in (BTM) , and this impairment lead to excess production of α - globin.This study conformed on patients having β - thalassemia major (BTM) .Sixty patients were chosen for this study complaining of profound paller , jaundice. They were clinically diagnosed on the basis of severe anemia and hemoglobin (Hb) electrophoresis.The aim of the present study was to investigate the lipid pattern in children with BTM having repeated blood transfusion which may lead to peroxidative tissue injury by secondary iron overload . The patients undergo irregular blood transfusion and desferioxamine was used as a chelating agent .Serum levels of total cholesterol (TC) , HDL - C , LDL - C , TGs, malondialdehyde (MDA),total lipid peroxides (LPO),iron , ferritin , copper , zinc, plasma total antioxidant capacity (TAC), ALT activity, and AST activity were measured .The results were compared with 40 age matched apparently healthy used as controls .BTM patients showed : significantly lower : TC , HDL - C , and LDL - C . whereas serum TG levels were found to be significantly higher than levels in control subjects. A significant increase in the levels of [(MDA), (LPO)], iron, and ferritin. Whereas a significant decrease in the level of total anti oxidant capacity (TAC) was observed. Serum levels of zinc were significantly decreased with significant increased in the levels of copper. Our results revealed that , lipid profile and other parameters above changed in patients with BTM . Many factors such as : iron over load , liver injury , and hormonal disturbances might cause these changes .It was concluded that , oxidative stress and reduced antioxidant defense mechanism play an important role in pathogenesis of BTM .

التوصيف الجزيئي لبعض عوامل الضراوة لبكتريا Moraxella catarrhalis المعزولة من اصابات الاذن الوسطى == Molecular Characterization Of Some Virulence Factors Of Moraxella Catarrhalis Isolated From Otitis Media

Author name: جمان خليل ابراهيم الصباغ
Supervisor name: محمد صبري عبد الرزاق | صفاء صاحب الغزالي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
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تقييم مستضد البروستات النوعي في مصل مرضى التهاب المسالك البولية == Evaluation Of Prostate Specific Antigen In Urinary Tract Infection Patients Serum

Author name: علي جبار عبد زيد الكلابي
Supervisor name: وسن سامي حميد
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
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العلاقة بين متلازمة مضادات التخثر لداء الذئبة ومضادات الكارديولبين ومضادات الدهون الفوسفاتية مع حالات الاجهاض المتكرر == Relationship Between Lupus Anticoagulant - Anticardiolipin - Antiphospholipid Anti Body Syndrome And Repeated Abortions

Author name: وسن اكرم ميخائيل
Supervisor name: زين العابدين عبد العزيز عبد الله
General topic: Medicine
Specific topic: Microbiology - Germs
Degree: Master
University: University of Al Mosul - Faculty Of Medicine
Language: English
University location: Mosul
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محاكاة كومبيوترية لتقليل الجرعة الاشعاعية مع الابقاء على نفس مستوى صورة المفراس لمرضى ذوي احجام متنوعة == Computer Simulated Dose Reduction With The Same Quality Of CT Images For Patients Of Various Sizes

Author name: فراس محمد عبد القادر
Supervisor name: عبد الرضا صاحب يونس الساعدي | شذى عبد الصاحب علش
General topic: Medicine
Specific topic: Medical Physics
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
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دراسة العلاقة بين الام الرقبة والام الكتف == Cervical Spine Pain And Its Association With Shoulder Joint Problems

Author name: نبيل احمد الطائي
Supervisor name: وميض رمزي العمري
General topic: Medicine
Specific topic: Diseases - Joints
Degree: Higher Diploma
University: University of Al Mosul - Faculty Of Medicine
Language: English
University location: Mosul
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رؤى فايروسية ومناعية في البيئة المجهرية لانسجة المشيمة للمجهضات المصابات بفايروسات الحلا == Virological And Immunological Insights Into Placental Tissue Microenvironment Of Aborted Patients Infected With Human Herpes Viruses

Author name: زينب عبد الحسين حامد
Supervisor name: سعد حسن محمد علي | باسم شهاب احمد
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
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دراسة دور العقدية الرئوية والرشاشية الدخناء في مرضى الربو : دراسة مصلية جزيئية == Study On The Role Of Streptococcus Pneumoniae And Aspergillus Fumigatus In Asthma : Serological And Molecular Diagnosis

Author name: اياد صالح مهدي
Supervisor name: عامر رشيد النجار | امنة نعمة الثويني
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
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المكورات العنقودية الذهبية المقاومة للمثسيلين بين العاملين في المستشفى والمجتمع وتميز وجود جين Luks_Lukf == Methicillin - Resistant Staphylococcus Aureus (MRSA) Among The Hospital Staff And Community With Focus On The Presence Of luks - lukf PVL Gene

Author name: الاء علي جواد
Supervisor name: عروبة خالد عباس | سراب خاشع الراوي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

العلاقة بين الانتجين النوعي للبروستات والجهد التاكسدي للنساء المصابات بمتلازمة تكيس المبايض في محافظة بابل == The Relationship Between Prostate Specific Antigen (PSA) And Oxidative Stress In Women With Polycystic Ovary Syndrome (PCOS) In Babylon Province

Author name: محمد مرزة حسن
Supervisor name: طارق حفظي الخياط | ملال محمد رضا الجبوري
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
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التغيرات الدموية والمناعية في مرضى التهاب الانف والجيوب التحسسي == Hematological And Immunological Changes Associated With Allergic Rhinosinusitis

Author name: حسين مهدي كاظم
Supervisor name: سعد مرزة حسين الاعرجي | صفاء حسين الطريحي
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:

العلاقه بين اللبتين والانسولين وحالة جهد التاكسدي عند مرضى السكري النوع الثاني ومرضى تكيس المبايض == Correlation Of Leptin, Insulin Resistance, And Total Anti - Oxidant In Type 2 Diabetic And Polycystic Ovary Syndrome Women

Author name: زينب يعسوب عجام
Supervisor name: مها فاضل سميسم | اسماء كاظم كاطع
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
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