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العلاقة بين كمية الخلية الحرة في السائل الجريبي وعلاقته بنوعية الجنين لدى النساء المصابات بتكيس المبايض
Author name:
هبة رشيد قاسم
Supervisor name:
حنان لؤي العمري | شيماء جمال احمد
General topic:
Medicine
Specific topic:
Physiology
Degree:
Master
University:
University of Baghdad - Faculty Of Medicine
Language:
English
University location:
Baghdad
First pages:
19T1411 - p.pdf
Abstract:
Poly cystic ovary syndrome is still consider as the most contra versal disease that affect females at reproductive age. It accounts about (5 - 10%) of females worldwide and can cause primary or secondary infertility. Cell free DNA is found as a result of apoptosis and lately it is used as an indicator for embryo quality. It is found to be present in follicular fluid as a result of folliculogenesis of granulose cell of oocyte and then can be measured in blood to determine the quality of oocyte and embryo in the in vitro fertilization procedures to have a better and higher success rate in these procedures, since in vitro fertilization are considered expensive procedures and they are the last hope for families to have children .Aiming of this study is to find a non expensive, most reliable and most accurate way to determine oocyte and embryo quality for a good result during in vitro fertilization procedure this through measurment of cell free DNA in follicular fluid and correlating it with embryo quality.Sixty females have been participated in this research were (32)of them having Polycytic ovary syndrome as a cause of infertility, while (28) of them are considered control group having un - explained infertility. All patient had passed through the antagonist protocol of ovarian stimulation preparing for intracytoplasmic sperm injection procedureBlood samples were drawn before and after stimulation for the hormonal measurements. Follicular fluid have been collected at day 14 at time of oocyte retrieval ,DNA extracted from this follicular fluid and cf DNA have been calculated by electrophoresis.Cell free DNA was with no significant difference between control (2.49±1.41 ng/μl) and PCOS patient (2.15±1.31ng/μl) . The good quality oocyte has significantly lower cf DNA level than that in bad quality one (1.04±0.5ng/μl vs 3.58±0.68 ng/μl for control and 0.89±0.65ng/μl vs 3.17±0.77ng/μl for poly cysticIXovary syndrome ) (p value =˂o.oo1). At the same time 17 ß - estradiol level was measured at time of oocyte collection and it was found to have a negative correlation with cf DNA (r= ¬ 0.033 and p=0.869 for control and r= - 0.0238 and p=0.18 for poly cystic ovary syndrome) . cf DNA was significantly lower in top quality embryo for both control and poly cystic ovary syndrome females (r= - 0.874 ,p=˂0.001 for control and r= - 912 ,p=˂0.001 for poly cystic ovary syndrome females).There is no difference in quantity of cf DNA in the follicular fluid for control and poly cystic ovary syndrome females .cf DNA is a good predictor of the embryo quality being higher in follicular fluid of oocytes yeilding poor quality embryos.
