الصفحات الاولى:

تاثير الصفائح النانوية على الخواص الفيزيائية والكهربائية لبعض المركبات البلورية السائلة Effect of Nanosheets On The Physical And Electrical Properties of Some Liquid Crystalline Compounds

اسم المؤلف: الاء فاضل سليمان داود

اسم المشرف: عصام عبد الكريم عبد اللطيف عمار هاني الدجيلي

الموضوع العام: علوم الكيمياء

السنة: 2014

الموضوع الدقيق: الكيمياء

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: هذه الاطروحة تمثل سلسلة من دراسات الكم من خلال التركيب الالكتروني للانظمة العضوية الفلزية. تتضمن نتائج الفصول خلاصة عمل تعود لكيمياء الروثينيوم (الفصل الثاني) واستخدام النظريات لتساعدنا على التشخيص التركيبي من خلال اجهزة الطيف الحاسوبية (الفصل الثالث والر | This thesis presents a series of quantum chemical studies into the electronic structure of organometallic systems. All of the results chapters are self - contained, summarising work related to Ruthenium chemistry (Chapter 2) and the use of theory to aid structural identification through computation of spectroscopic parameters (Chapters 3 and 4). Chapter 5 relates to distinct work done concerning to the Topological Analysis of the Electron Density in the dihydried Triruthenium Cluster, but the focus on the question of dihydried triruthenium motif represents a close link to the material covered in Chapters 3 and 4. In Chapter 2, Density Functional Theory (DFT) is used to probe the structures of triruthenium clusters Ru3(CO)12. The researcher shed light on the equilibrium geometries and the best function (PBE1PBE) and basis set (SDD Ru/ SVP on (C, O)) can be used. On the basis of that, the global minimum found for Ru3(CO)12 is an unbridged (1D3) structure. In Chapter 3, DFT with PBE1PBE functional and (SDD Ru/ IGLOIII, TZVP P, H/ SVP on (C, O)) basis sets have been used to identify structures of species observed in hydrogenation reactions of alkynes using catalysts based on Ru3 clusters. The identification of the hydride intermediates observed by NMR spectroscopy is ambiguous, and is generally based only on 1H NMR and JP - H coupling constants for hydrides attached to the ruthenium metal centres. Our calculations propose that the experimentally observed species A and B do not contain the Ru3(? - H)(H) motif, as proposed by Duckett group but have Ru3(? - H)2. In Chapter 4, these studies were extended to the analogous dihydride triruthenium monodentate and bidentate phosphines. By computing energies, 1H chemical shifts, we propose that the experimentally observed species A1 and B1 do not contain the Ru(µ - H)(H) motif, as proposed by Duckett and co - workers, but rather the dibridging Ru(µ - H)2 structures common to A2 and B2. In addition, the results confirm that our methodology is able to model the environment of a terminal hydride correctly.Finally, Chapter 5 describes the Quantum Aim (QTAIM) topological analysis of the electron density in the Picolyl N - Heterocyclic Carbene Triruthenium cluster [Ru3(? - H)2(?3 - ?3C2,NHCpyCH2ImMe)(CO)8] (3 - methylimidazol - 2 - ylidene). However, this cluster has been chosen since, firstly, has Ru3(? - H)2 motif similar to triruthenium core proposed in chapters 3 and 4 , secondly, this particular cluster contains one hydride - unbridged and two hydride bridged Ru - Ru edges and a face - capping ligands with different types of Ru - C and Ru - N bonds , this allowing interesting comparisons between the topological properties of related but different atom - atom interactions, within the same molecule.

دراسة كيميائية حياتية لانزيم اللاكتيت ديهايدروجنيز (LDH) ومتناظراته المنقاة جزئيا من ادرار المرضى المصابين باورام البروستات Biochemical Studies of Lactate Dehydrogenase And Its Isoenzymes Partially Purified From Urine of Patients With Prostate Tumors

اسم المؤلف: عمر علي كنوش الجبوري

اسم المشرف: فراح غالي الصالحي نزار احمد ناجي

الموضوع العام: علوم الكيمياء

السنة: 2008

الموضوع الدقيق: علوم الكيمياء

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية للعلوم الصرفة - قسم الكيمياء

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: تضمنت الرسالة ثلاثة فصول. يحتوي الفصل الاول على مقدمة لنبذة تاريخية للمواد البلورية السائلة وصفاتها الفيزيائية وبعض الامثلة لتطبيقاتها,وكذلك الخصائص الثرموديناميكية للمزيج الثنائي والثلاثي للمركبات البلورية السائلة. وتحتوي المقدمة ايضا على شرح مختصر لتق | This thesis consisted of three chapters. First chapter contained an introduction to explain the historical view, physical properties, application and thermodynamic characterization of binary and tertiary system mixtures of liquid crystalline materials. Also it contained an introduction to Nanotechnology and nanosheets like graphene oxide and its functionalization and a summary about the dielectric permittivity and conductivity. The second chapter explained the experimental part including the synthesis of graphene oxide (GO) and its functionalization and general synthesis procedure of eight liquid crystalline compounds, which were outlined as follows : 1. N1,N4 - Bis(4 - subst. - benzylidene) benzene - 1,4 - diamine [3a - d] 2. N4,N4? - Bis(4 - subst. - benylidene) biphenyl - 4,4' - diamine [5a,b] 3. 3,3' - dimethyl - N4,N4? - Bis(4 - subst. - benzylidene)biphenyl - 4,4' - diamine [7a,b] The binary mixtures were prepared from a non - polar compound N1,N4 - bis(4 - methylbenzylidene) benzene - 1,4 - diamine and N1,N4 - bis(4 - bromobenzylidene) benzene - 1,4 - diamine as polar compound. The tertiary mixtures were prepared by mixing the mole fraction of the eutectic point (60%A) mixture [D] from the binary system and considered as the first compound A, the second compound was 3,3' - dimethyl - N4,N4? - bis(4 - bromobenzylidene)biphenyl - 4,4' - diamine as compound B.The tertiary system mixture at (13%B) showed the lower eutectic point, each concentration of the eutectic points in the binary and tertiary systems was mixed with graphene oxide and graphene oxide functionalized with polyaniline. The third chapter described the characterization of the prepared GO nanosheets with different functional groups.These nanosheets were characterized with multiple techniques (FT - IR , X - ray diffraction, SEM and AFM). The prepared liquid crystalline compounds were characterized by using FT - IR infrared and the liquid crystalline properties of compounds [3a - d], [5a,b],[7a,b] , the binary, tertiary systems and the mixtures with graphene oxide (GO) and GO functionalized nanosheets were examined with hot - stage polarizing microscopy, at the concentrations of the eutectic points , measured the dielectric permittivity and conductivity ?ac at the concentrations of the eutectic points. The addition of the nanosheets caused lowering of the transition temperatures of eutectic points in the binary and tertiary systems. It is worth noting, that in the two mixtures was appeared a new unknown (enantiotropic smectic ) phase.

دراسة بعض القياسات الكيموحياتية والمناعية في مرض الفصام واضطرابات الاكتئاب الشديد في مدينة النجف الاشرف Study of Somebiochemical And Immunological Parameters In Schizophrenia And Major Depressive Disorders In Najaf Alashraf Province

اسم المؤلف: دعاء عبد الزهرة محمد علي الرماحي

اسم المشرف: حسين كاظم الحكيم

الموضوع العام: علوم الكيمياء

السنة: 2014

الموضوع الدقيق: الكيمياء

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية العلوم - قسم الكيمياء

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

اسم المؤلف: بشرى محمد كاظم ديوان البيضاني

اسم المشرف: عماد الدين عبد الهادي المختار

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

تاثير الاصابة بالاكياس المائية Hydatid cysts على مستويات مضادات الاكسدة في مصول المصابين The Effect of Hydatid Cysts Infection On Antioxidant Levels In Serum of Infected Individuals

اسم المؤلف: مريم ماجد محمد النصراوي

اسم المشرف: نغم ياسين كاظم البياتي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية في محافظة ديالى اذ تم جمع 100 عينة من اشخاص مصابين وغير مصابين بداء الاكياس المائية للفترة من 2013/10/1 الى 2014/8/10 من مناطق مختلفة تابعة لمحافظة ديالى والتي شملت (حمرين، المقدادية، قزانية، بعقوبة)، وقد توزعت العينات بواقع 54 من ا | The present study was carried out in Diyala province during the period from 1/10/2013 to 01/08/2014 in different areas including : Hamrin, Muqdadiyah, Kasaniya, and Baquba.Blood samples were collected from one hundred individuals who are at risk of hydatid cyst infection (veterinarians, shepherds, farmers, butchers and housewives).The diagnosis of hydatid cyst infection was carried out by Echinococcus IgG ELISA kit. It was shown that the total rate of infection was 16% (6% for male, and 10% for female). The study revealed that the highest percentage of infection was in individual live in urban compared with those who live in rural area.The mean concentrations of antioxidants ; glutathion, catalase and superoxide dismutase were measured in addition to measure the mean concentration of the oxidants.The results showed the presence of malondialdehyde in the serum of studied individuals. The results showed a decrease in the level of glutathion catalase and super oxide dismutase in infected individuals (0.0048±0.0596mmol/L, 0.0220±0.0260mmol/L, 0.0024±0.04430 mmol/L, respectively) compared with non - infected individuals (0.0237±0.2719mmol/L, 0.0024±0.0879mmol/L, 0.0428±0.3069mmol/L, respectively) while there was an increase in concentration of malondialdehyde (0.2245±2.915mmol/L) compared with non - infected (0.025±0.8042mmol/L). There was no significant differences in the concentrations of all these parameters according to age, sex and location of the cysts. The results showed that there was no correlation between each of catalase and superoxide dismutase and the malondialdehyde whil there was a negative correlation between glutathion and malondialdehyde. The study concludes that the infection of hydatid cyst leads to a decrease in antioxidants and to an increase in oxidants which may indicate that there was a damage caused by tissue infection.

انتاج وتوصيف متعدد السكريات الخارج خلوي Lactobacillus plantarum من بكتريا Exopolysaccharide المعزولة محليا ودراسة فعاليته البيولوجية Production And Characterization of Exopolysaccharide (EPS) From Locally Isolated Lactobacillus Plantarum And Studying Its Biological Activity

اسم المؤلف: بيداء مهدي عباس الغانمي

اسم المشرف: ناجح هاشم كاظم الظويهري

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: ماجستير

الجامعة: جامعة كربلاء - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: كربلاء

الصفحات الاولى:

المستخلص: Twenty - five isolates of Bacillus spp. Were isolated from 15 soil samples collected from different places in the Holly city of Kerbala, 13 isolates lactobacillus spp. Were isolated from 15 milk products and 7 isolates pseudomonas spp. The isolates were screened for their ability to produce Exopolysaccharide.The results showed that isolate number 3 (Lactobacillus lac 3) was found to give the highest production of the polymer, the isolate has been identified as lactobacillus plantarum. The optimal cultural and environmental conditions for EPS production were been identified which include using the production medium (simplified synthetic medium) containing date juice (5%) as a carbon source and yeast extract (0.2%) as a nitrogen source, the media was enhanced with a total concentration 0.9% of magnesium sulphate and potassium phosphate as mineral salts. The primary pH was adjusted to 6.5 and the inoculum size was 3%(v/v), isolates were grown under anaerobic condition at 35 Co for 24 h and the EPS extracted with ethanol 95%. Two steps were used for partial purification of the polymer as follows, the first step by using TriChloroAcitic acid (TCA) solvent and the second step by dialysis. The yields were (77.4 and 63.8)% for the two purification steps respectively. The biological activity of the EPS extraction from L. plantarum has been tested against some species of G+ve and G - ve bacteria and some fungal. The results showed that EPS extrac exhibits an inhibitory activity against Stenotrophomonas, Bacillus cereus and Proteus bacteria with an inhibition zone diameter of 23, 21, 19 mm respectively. While, there was no inhibitory effect against fungi. Antioxidant activity has also been tested, where IC50 value against ABTS free radical at 100 Mg / ml. The characterization of partially purified EPS is investigated by using Fourier Transform Infrared Spectroscopy (F.T.I.R). The molecular weight of the polymer was also measured by using viscometer and the molecular weight obtained by this technique was 2.3 *105 Dalton. The EPS extraction from selected isolate showed two spots when separated by Thin Layer Chromatography (TLC) which is analyzed as a glucose and galacto

اسم المؤلف: احمد عطا الله حسن الفهداوي

اسم المشرف: عادل مشعان ربيع

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: البيئة المائية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

العلاقة بين مؤشر الموت الخلـوي المبرمج (ربيطة فاس الذائبة) وقلة النطاف عند الرجال Correlation Between Apoptotic Marker (Sfas Ligand) And Oligozoospermia In Men

اسم المؤلف: احمد حسين علي الزاملي

اسم المشرف: صباح ناصر العلوجي انعم رشيد الصالحي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: The Fibroblast associated (Fas) system in the testes has been identified as a key regulator of apoptosis, a process that greatly influences the germ cell population of the testes. and the soluble Fas Ligand (sFasL) may be a competitive inhibitor to induce apopto¬sis of the Fas baring cells. Therefore, this study measures the level of sFasL in the semen of oligozoospermic men evaluating the association between seminal plasma sFasL and spermatogenesis.A total 58 oligozoospermic men and 29 normozoospermic volunteers were included in this study. They were attending the Infertility Clinic of the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, AL - Nahrain University\ Baghdad. Their age mean was 31.77± 5.48 years and a median of 30 years. Semen samples were evaluated according to World Health Organization 2010 standard parameters. The Seminal plasma was separated from the cellular elements after centrifugation and the supernatant was transferred to plane tubes and frozen at - 20 ?C till using for measurement of sFasL. These patients were classified into patients with severe oligozoospermia (sperm concentration ?5 million / ml) which include 29 patients, and patients with mild and moderate oligozoospermia (sperm concentration ranging between 5 - 15 million / ml) which includes 29 patients as well. The control group comprised of 29 men with normospermic parameters according to WHO 2010 standard (Normal volunteers). The sFasL was measured using ELISA enzyme immunoassay for quantitative determination of sFasL Kit. Data were analyzed statistically using Analysis of variance (ANOVA), Least Significant Difference (LSD) and Correlation Coefficient (r).The sFasL level was found significantly higher (P< 0.01) in seminal plasma of oligozoospermic men, with weak correlation of the level of sFasL with the degree of severity of oligozoospermia. Based on the results of this study, it was concluded that sFasL is a novel marker found in the seminal plasma of oligozoospermic men. It's level was higher in cases of oligozoospermia regardless of its severity.

انتشار بكتريا Escherichia coli في المرضى في المرضى المصابين بالتهاب المرارة الحصوي في محافظة الديوانية Dissemination of Escherichia Coli In Patients With Cholecystitis Calculus In Al - Diwanyia Governorate

اسم المؤلف: احمد رزاق واجد الداوودي

اسم المشرف: سيوف خومان علوان الرماحي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: The study aims to identify Escherichia coli bacteria, which causes calculus cholecystitis of molecular perspective. The study samples were gathered from 110 patients who undergone cholecystectomy in Diwaniya general hospital for the period October 2013 to April 2014. The sample included bile sap, gall bladder tissues and stone if available. The samples were cultured to identify their culture specifications.The results showed that chronic cholecystitis is the most common one, which has 55.5% then acute cholecystitis by 44.5%. The study results showed that age and sex have an influence on cholecystitis. The highest percentage of infection is the ages 36 - 45 for the two sexes. If the cases distributed according to the patient sex, there is a difference in cholecystitis between male and female, where females infections are 88.2% and males are 11.8%. The total isolated bacteria were 125 bacterial isolates of 52% of gall bladder tissues, 26.4% of bile and 21.6% of the stone. Escherichia coli had 34.4% of the total samples of 51.1%, 36.6% and 29.6% respectively of the above mentioned samples.As far as susceptibility for all Escherichia coli isolates towards 15 types of antibiotics by using disc diffusion for Kerby - Bauer method, bacteria had high susceptibility against ? - lactamases antibiotics where they had susceptibility against Ampicillin+ Clavulanic acid by 38.7% and 79% for Ampicillin. The least susceptibility was for Amoxicillin by 69.7%. Bacteria had no resistance for Imipenem where their susceptibility was 100%. Bacteria had weak susceptibility against Aztreonam by 13.9%. While with cephalosporins, susceptibility for Ceftazidime by 27.9% and for Cefotaxime by 20.9%. With Aminoglycosides, resistance to Gentamicin antibiotics was 34.8% and 30.2% for Amikacin. The results showed that resistance to Quinolones was 16.2 for Nalidixic acid while for fluoroquinolones resistance was 25.5% for Ciprofloxacin and 18.6% for Norfloxacin. Isolates ability to produce CTX - M, SHV, TEM and AMPC extended spectrum ? - lactamases enzymes was tested by investigating the existence of blaTem, blaAMC, blaCTX - M and blaSHV genes in these isolates through Polymerase chain reaction (PCR). There were 23/26 isolates (88.4%) showed their ability to contain blaAmpC gene, and 7/26 isolates (26.9%) contained blaSHV gene. There are 18/26 isolates(69.2%) contained blaTEM gene while 20/26 isolates (76.9%) contained blaCTX - M.Through using Quantitative Real Time - Polymerase chain Reaction (qRT - PCR), gene expression measurement for blaCTX - M, blaTEM and blaAmpC was measured by relative numbers. The results showed that gene expression measurement for ? - lactamases enzymes type AmpC produce by Escherichia coli was the highest if compared with ? - lactamases enzymes type TEM and CTX - M with different periods of incubation, which is T4h for 4 hours, T24h for 24 hours and T48h hours of incubation respectively. It is noticed that genes responsible for producing ? - lactamase enzymes type TEM, CTX - M and AmpC are changed during different periods of incubation through using Real Time - Polymerase chain Reaction (RT - PCR).

دراسة بعض الملوثات الصناعية وتاثيرها في الصفات التشريحية لبعض الانواع النباتية في قضاء سامراء Study of Some Industrial Pollutants And Their Impact in The Anatomical Characteristics of Some Plant Species in Samarra

اسم المؤلف: احمد يونس حمادي الكنعاني

اسم المشرف: طالب عويد الخزرجي ياسين محمد احمد الدليمي

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: ان نمو النباتات في مواقع ملوثة قد يعرض صحة المستهلك للخطر سواء اكان انسان? او حيوان?، لذلك انجز هذا البحث لتقييم التلوث بالعناصر الثقيلة في مياه المخلفات الصناعية للحي الصناعي في مدينة سامراء وفي التربة والنباتات الواقعة على مجرى هذه المياه الجارية في ساق | The growth of plants in contaminated sites may expose consumers' health at risk, whether a human being or animal so accomplished this research to assess the pollution heavy elements in the waters of industrial waste of the neighborhood's industrial city of Samarra, in soil and plants located on the course of this running water in a stream length of 800 m even downstream Tigris River, as they are put waste industrial district as well as sewage water into the river is directly without treatment, as the study was to take part of the water and soil of that region as well as four types of plants that grow where is the tail of the seven, mint, sugar cane and corporal parents, study Some qualities Physical and chemical soil and water in addition to study some changes anatomical got to plants due to pollution, and that by dividing the study area into four zones as is the region (1) part near the industrial district and region (2) is located just 200 meters from the site of No. (1) towards the river, followed by site number (3), which is 200 m from the site of No. (2) The region (4) is located near the bank of the river and about 200 m from the site number (3) and samples were taken control of the West Bank of the Tigris River, was measurement of both pH and electrical conductivity and salinity in addition to measuring some heavy elements (iron, copper, cadmium) of soil and water, as well as the measured requirement vital for oxygen and the degree of turbidity of the water, also included the study to measure some heavy elements (iron, copper, cadmium) in plant and study the anatomical changes that occur due to the pollution produced by the launch of industrial waste. The results of the study that there is variation among study sites for each of the regions of the east bank of the river (the contaminated samples) and the West Bank of the river (the control) for most attributes Physical and chemical water and soil as well as for changes anatomical plants between contaminated samples and the control and in general has The results are within the limits permitted by the World Health Organization, the results indicate the high concentration of copper in the soil compared with iron and cadmium as the highest concentration has 10 ppm at the site (1), while the concentration of sample control 0.17 ppm The iron was higher focus him in the soil is 5.2 ppm in both locations (3) and (4), while the concentration of sample control 3.3 ppm The cadmium was the highest concentration has 0.43 ppm at the site (2), while the concentration of sample control 0.15 ppm and these concentrations are all not exceeding the allowable limit for the World Health Organization (for cadmium 1.5ppm of iron 120ppm copper 40ppm) respectively, and the results showed variation in pH between the soil and water for study sites on the one hand and sample control on the other hand, as shown soil samples to pH moderate among the four sites studied, where ranged pH between 7.26 - 6.33 The sample control was pH 8.64 As for water, the results showed basal water sites studied ranged pH between 6.8 to 5.8 which is comparable to Las pH for sample control 8.5, As for connectivity electrical has emerged variation between samples studied and sample control for soil and water and showed samples of soil and water at the site (2) the extent of the variation connectivity electrical them at the sites (3, 2, 1) and which are all higher than in the sample control, with varied degrees connectivity for soil samples between 14, 200 - 2860 Maekerosemins / cm The sample control 2300 Maekerosemins / cm The degree connectivity for water samples 2810 - 2450 Maekerosemins / cm The sample control of water was connectivity 1457 Maekerosemins / cm, for salinity, the results showed an increase in salinity of soil samples and water compared to a sample control ranged salinity in the soil between 8.92 - 1.79 g / l, while a control sample was salinity 1.438 g / l The salinity in the water was between 1.759 - 1.532 g / l The control sample was 0.907 g / l The results showed that the sample at the site (2) the degree of salinity have is higher than the rest of the other samples in soil and water. Results showed high copper for iron in the water was the highest percentage of copper in the signatories (4), (3) 0.05ppm The sample control has, (2.1) compared to a control sample, where he was between 55 - 20 mg / L, while a control sample 3.8 mg / liter, while for water Ekorh has ranged between four samples where Ekorh was water between 12.36 - 2.97 naphthalene The control sample was Ekorh water 2.1 Naphthalene. The results showed that the concentration of iron was high in most plants for copper and cadmium, as was the highest concentration of iron in the plant reeds 24 ppm as was assembled in the root zone of the plant which is within the limits permitted by the World Health Organization while the highest concentration of iron in the area of control of the plant reed it reached 17.5 ppm as it was assembled in the root also Copper was the highest concentration in the plant sergeant cock 0.89 ppm as it was assembled in the root while the focus was in control very few samples The cadmium is 0.1 ppm in all plants is within the allowable limits by the World Health Organization, while in the side anatomical has emerged is a difference between the samples studied and samples of control, as happened increase in the dimensions of the vascular bundles of the leg, as well as an increase in the thickness of the crust in the samples studied compared a sample of control as well as an increase in the thickness of the tissue foundation and a decrease in the number of packets Vascular plant roots as well as increase the thickness of the securities, an increase of pollution in the studied plants compared to a control sample..

التنميط الجيني لفايروس التهاب الكبد نوع اي لمرضى الاصابة الحادة في محافظة المثنى - العراق Genotyping of Hepatitis E Virus Among Patients With Acute Infection In Al - Muthanna Province / Iraq

اسم المؤلف: ازهار صبري مسلم الذهبي

اسم المشرف: رغد حربي مهدي العزاوي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - الفيروسات

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: Hepatitis E Virus (HEV) infection is found worldwide. It is responsible for large outbreaks in East and South Asia. This study was carried out to identify the distribution of acute HEV infection in AlMuthanna province and then determine the genotypes responsible for the disease spreading. This is the first investigation about the occurrence of hepatitis E genotypes in Al - Muthanna province patients. The study included 270 jaundiced patients of both sexes From AlMuthanna province which included out patients in public health laboratory in Samawah city, for the period from October 2013 to June 2014. All these patients were tested for anti - HEV IgM antibodies by using enzyme linked immunosorbent assay (ELISA). In Al - Muthanna province, 72 patients (26.66 %) out of 270 patients showed positive results for anti - HEV IgM antibodies, and all those patients were tested for confirmatory test at central public health laboratories (CPHL) in Baghdad province. Those patients consist of 45 females and 27 males with age ranged between (4 - 74) years old, were all negative for routinely screened markers of Hepatitis A, Hepatitis B and Hepatitis C, Ten normal healthy individuals, used as normal control in this study. This study showed that the HEV IgM is more common among younger age group (15 - 24), with a percentage of) 41.67%(, and it was higher in females (63%) than in males (37%). Highly significant differences (p ? 0.01) appeared among age groups. The levels of liver function enzymes demonstrated significant differences (p ? 0.05) in HEV patients as compared with healthy controls. The levels of Total Serum Bilirubin (TSB) were (23.41 ± 12.38) mg/dl, Serum Aspartate transaminase (SAST) were (887.17 ± 9.87) U/l, Serum Alanine transaminase (SALT) were (859.91 ± 13.28) U/l, and the levels of Serum Alkaline phosphatase (SALP) were (206.56 ± 5.04) U/l higher than healthy controls (0.884 ± 0.05) mg/dl, (37.27 ± 4.58) U/l, (34.14 ± 7.63) U/l, (48.09 ± 15.42) U/l respectively. RNA was extracted from sera of positive anti - HEV IgM antibodies by using a QIAamp® Viral RNA Mini Kit. Nanodrop and Quantus™ Fluorometer instruments were used to determine the RNA yield and purity according to the standard kit protocol. High levels of RNA concentration were measured by Nanodrop and Quantus™ Fluorometer. Forty - six samples have high RNA concentration out of 72 samples were detected for genotypes I, III & IV by using Applied Biosystems Real time PCR 7500 machine. In this study, HEV type I and III were detected in 23 samples out of 46 samples by using Real Time PCR systems. Ten samples were positive for this test. Thirteen samples (56.52%) were negative for this test. The distribution percentage of genotypes I and III was (43.48%). The results appeared significant difference (P ? 0.05). Also by using the same technique (Real time PCR systems) the HEV type IV was detected in 23 samples out of 46 samples. Which included, 13 samples that recorded negative results by I & III genotypes kit and only 2 samples from it recorded positive results and 11 samples recorded negative result for HEV genotype IV. From other 10 samples, only 3 samples recorded positive result and 7 samples recorded negative result for HEV genotype IV. In a total, 5 samples were positive for HEV genotype IV and 18 samples (78.26%) were negative. So that the distribution percentage of genotype IV was (21.74%). In this study, the samples that showed negative results in both kits have not been tested for other genotypes. This study indicated that the acute HEV infection is highly endemic in Al - Muthanna province in Iraq. Genotypes I & III were the most distribution than genotype IV in patients from Al - Muthanna province in Iraq. These results suggest that the genotypes I & III are the main causative agents of sporadic HEV infection in Al - Muthanna province

تاثير بعض العوامل البيئية في مجتمع الهائمات القشرية لنهر دجلة وجزيرة بغداد السياحية - العراق The Effect of Some Environmental Factors In Crustaceans Plankton Community of The Tigris River And The Island of Baghdad Tourist - Iraq

اسم المؤلف: اعتدال فليح حسن العامري

اسم المشرف: صباح فرج عبد الاحد

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان - اللافقريات

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: The present study was conducted to investigate the effect of the water body type on the density of crustaceans zooplankton of the cortical Cladocera and two orders of copepod (Cyclopoida, Calanoida), addition to the physical and chemical effect of lake of Baghdad Tourist Island and Tigris river within the water of the lake near the site and electric and know the effect these characterties on density of crustaceans Zooplankton and for doing this study. We choose five places, tow on Tigris river and three places on the Island in AI - Fahhama in north of Baghdad The samples of water were collected every 15 days all these stations from beginning of December 2013 to May 2014. This study had corrected the temperature of air and water and the pH, electrical conductivity. and Dissolved oxygen, BOD5, NO?¯, PO?¯ and the density of Crastaceans Zooplankton. This study showed that the temperature of the air was nearly in the river and the lake and that because of their being in the same geographs area.The temperature was between (16°c - 34°c)in Tigris while it was (11.9°c - 33°c)in the lake Hydrogen Ions, it was (7.3 - 7.9) in the river and (8.18.8)in the lake because that were a lot of water plants and phytoplankton. This study shows that water of the river and the lake in the areas of study have a good of air and that was shown by recording the heigh values of oxygen. The values of dissolved oxygen (DO) was between (8.11 - 11.5) mg\L in river between (7.9111.37)mg\L in lake and showed from the study that the values were few. It was less than 5mg\L it was (0.33 - 2.6) mg\Lin the river and (0.64 - 3.55) mg\L in the lake. The values electrical conductivity of water in all the placed the study in the permissible range the water, it isn't over than 1500?s\cm. The electrical conductively records were between (851 - 1184) ?s\cm in the river, and it was (755 1270) ?s\cm in the lake. From the other hand nutrients, the phosphor records between (0.025 - 0.0563)mg\Lin the river and in the lake between(0.00310.0429)mg\L The Nitrate records its values highs in the month of winter because of the rain and it was increased in the river, it was (0.345 - 0.9135)mg\L compare with the lake, it was (0.028 - 0.626)mg\L in the places of the study that were appearance of two clear increases in the density of Zooplankton one was in winter and the other was spring.The density of zooplankton in the lake much more than it was in the river. The density of the zooplankton belong to Cladocera between (0.019 - 0.307) ind.\L in the lake and the density of the Calnoida was between (0.003 - 0.065)ind. \L in the river and (0.0078 - 3.37) ind. \L in the lake. The results shows differences if there are changes in places and month on most of physical and chemical biological characteristic the measured during the period of this study. The kind of water body effects on most characteristics and soon the density of crustaceans zooplankton.the results of the study mention the binding morale relation between the density of zooplankton the values of electrical conductivity and value of BOD(Biological Oxygen Demand) while there isn't any morale relation between the density of zooplankton and the value of Hydrogen Ion and Nitrate and dissolved oxygen that because the value of these changes was near to be equal in all the places that eayses the reduce of its effective on the average of density of these organism. The study shows that Baghdad lake have the environmental ingredients makes the lake natural protected to forming and breeding a fish in the lakes in addition that it is a place for Tourism.

تاثير عصارة نبات البروكلي في متلازمة تعدد الاكياس المبيضية المستحدثة في الجرذ الابيض Effect of Broccoli Plant Juice On Induced Polycystic Ovarian Syndrome In Albino Rats

اسم المؤلف: رقية احمد صالح

اسم المشرف: نهلة جاسم محمد الشاهري

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان - الفسلجة

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: The goal of present study was to investigate the benefits of broccoli as anti androgenic plant in protection and treatment for reproductive hormonal and metabolic disturbance, which combined with polycystic ovarian syndrome (PCOS). Three experiments for 3 PCOS models were done. First Experiment : The prepubertal female rats at 21 days old, were injected with Testosterone (T) subcutaneously for 39 days (first model). The females of this experiment were divided into 6 groups (8 rats each) as follows : Group 1 was injected with sesame oil. Group 2 was injected with T hormone. Group 3 was injected with T hormone with orally gavaged of broccoli juice. Group 4 was injected with sesame oil, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 5 was injected with T hormone, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 6 was injected with T hormone, and at the end of the last injection was orally gavaged with broccoli juice for 30 days. Second Experiment : The prepubertal female rats at 21 days old, were injected with Letrozole (L) subcutaneously for 39 days (second model).The females of this experiment were divided into 6 groups with the same style of the first experiment. Third Experiment : it was achieved by the injection of pregnant rats prenatally (days 16 to 19 of gestation) with 1 mg of T hormone subcutaneously (third model), and then the female offspring were divided into groups (8 rats each) as follow : The first offspring group : The female rats offspring at 21 days old (their mothers were injected with sesame oil from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with distilled water : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with broccoli juice : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with broccoli juice until 60 days of age. The third offspring group : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation and orally gavaged with broccoli juice) were orally gavaged with distilled water until 60 days of age. Females of all groups were sacrificed after 24 hours from the last treatment to undertake weight, histological, hormonal and metabolic study for each group. the results of present study showed the symptoms that reported PCOS of the three female experiments such as ovarian cyst, hyperandrogenemia, hormonal and metabolic disturbance, etc. Also a well improvement has been noticed in all syndrome symptoms on females gavaged with broccoli juice for all three rats PCOS experiments, these results indicate that broccoli juice has got a protective and curative role against this syndrome.

دراسة بكتريولوجية لاخماج المسالك البولية للنساء الحوامل والمصابات بارتفاع ضغط الدم وداء السكري في منطقة تكريت Bacteriological Study of Urinary Tract Infections Among Pregnant, Hypertensive, And Diabetic Women in Tikrit Region

اسم المؤلف: اسراء عز الدين ابراهيم

اسم المشرف: عبد الكريم فتاح عمر

الموضوع العام: علوم الحياة

السنة: 2010

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: النساء المتزوجات والحوامل والنساء المصابات بداء السكري وضغط الدم المرتفع ومدى مقاومتها للمضادات الحيوية لقد اشتملت الدراسة على 411 عينة ادرار من المريضات المراجعات لمستشفى تكريت التعليمي و100 عينة ادرار كمجموعة ضابطة للفترة من تشرين الثاني2008 ولغاية ايلو | This study aimed to isolate and identify the bacterial causative agents of urinary tract infections among married, pregnant, diabetic and hypertensive women and to determine the susceptibility of isolated bacteria to different antibiotics. The study included 411 urine samples for women attended to Tikrit Teaching Hospital, besides 100 urine samples used as a control group. The study carried out for the period from November 2008 to September 2009.E. coli was the most isolated bacteria at the rate of 31.8 % from married women followed by Staphylococcus saprophyticus 18.18 % and Staphylococcus aureus 15.90%. In the pregnant women, S. saprophyticus was the most isolated bacteria 48.10 % followed by E. coli 20.25 % and S. aureus 12.65 %, and in the diabetic women, S. saprophyticus was the common isolated bacteria 32.07 % followed by E. coli 28.30 % and S. epidermidis 15.09 %. In the hypertensive women, S. saprophyticus 30.35% was the common isolated bacteria followed by E. coli 25.0% and S. aureus 19.64 %. In the case of diabetic pregnant women, and in the hypertensive pregnants S. saprophyticus was the most isolated bacteria, (80 % and 75 % respectively), while E. coli 44.4% was the common isolated bacteria among the diabetic hypertensive women. Out of all isolates, S. saprophyticus 35.13 % came in the first rank followed by E. coli 26.64% and S. aureus 12.74% while Klebsiella oxytoca and Serratia odorifera (0.38% for each of them) were the lowest isolates. Urinary tract infection was common among urban patients 57.49%.The isolated bacteria showed different susceptibility to the antibiotics used in this study. S. saprophyticus isolates showed high sensitivity to Amikacin, Nitrofuratoin and Cefolaxime (the resistance rate for these antibiotics were 11 %, 12.1 % and 12.1 % respectively). While these isolates showed high resistance to Streptomycin 76.9 %, Nalidixic acid 67 % and Cephalexin 69.29 %. E. coli isolates showed low resistance rate to Amikacin 9.28 %, Ciprofloxacin 10.1 %, whereas showed high resistance rate to Cephalexin 47. %, Streptomycin 84 % and Ampicillin 82.6 %. The isolates of S. aureus showed low resistance to Ciprofloxacin 18.2 %, Carbencillin 18.2 % and Amikacin 15.2 % and high resistance for Ampicillin 18.8 %, Nalidixic acid 75.8 % and Cephalexin 72.79 %. Other isolated bacteria showed a different percentages of susceptibility to antibiotics Serratia odorifera, Proteus vulgaris and Enterococcus faecalis showed a significant resistance to antibiotics, while S. epidermidis isolates showed low percentages of resistance to Ciprofloxacin 9.1 %, Amikacin 18.2 % and Gentamicin 9.1 %. For S. saprophyticus the minimal inhibitory concentrations (MIC) resulted from Ciprofloxacin, Gentamicin and Cefotaxime were 8 - 32, 8 - 64 and 4 - 8 ?g/ml, and for E. coli the MIC results were 2, 8 - 16 and 16 - 64 ?g/ml. The MIC results were 2 - 8, 8 - 128, 32 - 256 ?g/ml for S. aureus, while the MIC results were 8 - 32, 16 - 64 and 16 - 128 ?g/ml for Klebsiella pneumoniae.

تاثير مستخلصات المركبات التربينية والقلوانية والفينولية الخام لنبات الزريج Chrozophora tinctoria L. في بعض جوانب الاداء الحياتي لحشرة الذبابة المنزلية Musca domestica L.(Diptera : Muscidae) مع عزل وتشخيص المركبات الفعالة باستعمال تقنية الكروموتوغرافيا الس The Effect of Crude Extract For, Terpenoids Alkaloids And Phenolic Compounds of Chrozophora Tinctoria L. On Some Biological Aspects of House Fly Musca Domestica L. (Diptera : Muscidae) And Isolation And Identification of Active Compounds By Using High Per

اسم المؤلف: اسيل كريم جبار السلطاني

اسم المشرف: هادي مزعل خضير الربيعي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: اجريت سلسلة من التجارب المختبرية في مختبر بيئة الحشرات/كلية العلوم للبنات/جامعة بابل خلال عام 2014 - 2015 تضمنت اختبار فعالية مستخلصات المركبات التربينية والقلوانية والفينولية الخام لنبات الزريج Chrozophora tinctoria في بعض معايير الاداء الحياتي لحشرة الذ | A series of laboratory experiment were conducted in the laboratory of insect /college of Science for women /University of Babylon during the year 2014 - 2015 The present study conducted to evaluate the activity of crude extract of Terpenoid, Alkaliod and Phenolic compounds of Chrozophora tinctoria on some biological aspect of Musca domestica and histological study for effect crude phenolic compound on ovaries as well as isolation and identifaction of active compounds in these extracts.The extract of crude terpenoid compound was more effective than crude alkaloid and phenolic compound in different critirra of biological perphormance. The mortality rates of eggs in the extract of terpenoid alkaloid and phenolic compound at concentration 1mg/ml were (90, 63.7, 39.6)? respectively compared with (0.4, 0.8, 0.8)% in the control tretmentsThe mortality rate of first larval instar was (57.64, 57.00, 26.85)% respectively when treated with crude terpenoid, alkaloid and phenolic compounds at concentration 1mg/ml compared with (0.8, 0.8, 0.8)% in the control treatment.The mortality rate of pupae was 18.06% when treated with crude terpenoid extract at concentration 1mg/ml compared with 0.4% in the control treatment. The cumulative mortality rates of immature stage when treated with crude terpenoid, alkaloid and phenolic compound were (100, 100, 42) % at concentration 1mg/ml compared with (15, 14, 12)% at the control treatment.Developmental period of immature stage was 36.6 days when treated with crude alkaloid extract at 0.25 mg/ml compared with 10.2 days in the control treatment.The pupal weight and female productivity were reduced from 0.1gm, 122.6 egg/female in control treatment to 0.556 gm, 0 egg/female respectively when treated with crude alkaloid extract at 0.25 mg/mlThe different concentration of crude terpenoid, alkaloid and phenolic compound were effective in the performance of eggs deposition, the nummber of eggs decreases in crude extract terpenoid, alkaloid and phenolic compounds at the concentration in same space was (0) egg/femal for all extracts at concentrion 1mg/ml comparted with (117, 118, 106) egg/femal in the control treatment, while was (0) egg/femel when treated in different concentration of terpenoid, alkaloid alone of consentration 1mg/ml compared with (120, 122) egg/femel in the control treatment.The number of eggs was (37) egg/femel when treated with phenolic compounds at same concentration compared with (120) egg/femel in the control treatment.The study included the effect extracts activity for crude phenolic at concentration 1mg/ml in histological structure for insect overies, The length and diameter of ovary and ovares follicles at (24, 96) houres after emergency while the length and diameter of ovary was (625, 468.75)µm respectively at 24 houre at concentration 1mg/ml compared with (652.173, 565.217)µm in the overy of femel in the control treatment. The length and diameter of ovaries follicles were (116.66, 72.22)µm respectively in the femel overy for control treatment, while was not distingwish for follicles female which were produced from treatment. while in (96) hour after emergency the length and dimeter of ovary (1114.285, 971.428)µm respectively when treated with extract compard with (1688.88, 1481.48)µm for overy of female in control treatment.Isolation and identification of active compounds in the crude extract of terpenoid, alkaloid and phenolic compounds, of Chrozophora tinctoria by using high performance liquid chromatography (H.P.L.C), Five compounds isolated from crude terpenoid extract was Teter methyl hydrazine compound which had retention time(1.333) mintue which was the first compound, while seven compounds isolated from phenolic compounds The gallic acid compound had retention time 1.34 mintue, which was the first compound isolation.Two compounds were isolated from crude alkaloid compounds which were berberine and isoquinoline, berberine compound had retention time 2.51 mintue which was more concentration than other compound.

دراسة تصنيفية لبعض اجناس العائلة المركبة Asteraceae في العراق Taxonomical Study of Some Genera From Asteraceae In Iraq

اسم المؤلف: اشواق فالح خزعل العجيلي

اسم المشرف: عبد الكريم خضير البيرماني

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: تناول هذا البحث دراسة تصنيفية مقارنة لستة اجناس تعود للعائلة المركبة Asteraceae وهي Chrysanthemum L. وGazinia وGerbera وLeontodon L. وTagetes L. وZinnia L. وتميز كل جنس من تلك الاجناس المذكورة باحتوائه على نوع واحد فقط في العراق وهيL. Chrysanthemum coro | The present investigation comprises systematic study for six genera belong to Asteraceae as follow : Chrysanthemum L., Gazinia, Gerbera, Leontodon L., Tagetes L. and Zinnia L. Each genus of those genera described as containing only one species in Iraq, Chrysanthemum coronarium L., Gazinia splendens Gaertn, Gerbera jamisoni F.Gmel, Leontodon laciniatus L., Tagetes patula L. and Zinnia elegans L. respectively. The study was conducted in the laboratory research of plant taxonomy / College of Science women / Babylon university for the period January 2015 until August 2015.The study included morphological, anatomical, palynological and chemical characteristics as well as numerical taxonomy have been done for all species. The importance of taxonomic to studying variations in roots, stems, leaves, inflorescences, involucre bracts, flowering and fruiting parts and Indumentum were discussed. Results showed that many of the vegetative and reproductive characters have the importance taxonomic value for isolating genus from each other such as presence and absence of the stem and the nature of the basal and cauline leaves in addition the present of leafy stipule as well as the nature of Indumentum.Anatomical characters of leaves epidermises and the cross section of the stem, leaf blade, petiole and peduncles, in addition leaves venation have been studied and their taxonomic importance were assessed.The study also found the pollen grain was tricolporate in general, but the presence of spine or not had an important taxonomic to isolate Gerbera jamisoni by smooth surface compared to the rest of the species, while possible to isolate Gazinia splendens and Leontodon laciniatus by the presence of the bridges in pollen surface. On the other hand it has been the study of chemical content in each of the leaves and flowering heads of the species studied using Gas Chromatography technique (GC) to detect the terpenoid compounds, The presence of compound Nerolidol, Terpinolene in the species.. Gerbera jamisoni, Zinnia elegans emphasized the importance taxonomic chemical content at the level of species and genera.The species were numerically treated on the basis of number of selected characteristic from various studies to obtain the similarity and differences ranges between different species. Dendrogram was drawn for this purpose. As a result of preceding observation this study confirmed the diagnosis of six genera within Asteraceae family in Iraq. Finally all the taxa were treated taxonomically, and the key was prepared for all the taxa studied.

التحري عن التشوهات الكروموسومية وطفرات المورث FLT3/ITD في مرضى ابيضاض الدم النخاعي الحاد Screening For Chromosomal Aberrations And Gene Mutations FLT3/ITD In Patients With Acute Myeloid Leukemia

اسم المؤلف: سمارة كاظم محمد

اسم المشرف: عبد الحسين مويت الفيصل

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: التقنيات الاحيائية

الدرجة: ماجستير

الجامعة: جامعة بغداد - معهد الهندسة الوراثية والتقنيات الاحيائية - قسم التقانة الاحيائية

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: صممت الدراسة الحالية من اجل تسليط الضوء على تاثيرات مرض ابيضاض الدم ((AMLفي بعض معايير الوراثة الخلوية والجزيئية الحاصلة في كريات الدم البيضاء في ثلاثة حالات للمرضى اشتملت على مرحلة ما قبل العلاج, في اثناء العلاج وبعد العلاج الكيميائي. كما شملت الدراسة ا | The present study was designed to shed light on the cytogenetic and molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three stages before, during and after treatment with chemotherapy (in vitro) in lymphocytes. It was also aimed to investigate chromosomal aberrations, micronucleus formation and FLT3/ITD point mutations in CN - AML region 100 - 300 bp compared with healthy control. The study was comprised of forty - seven AML Iraqi patients their ages ranged between 2.5 - 81 years. It included twenty females and twenty seven males compared with twenty - six healthy control. The following results were obtained : AML is most common leukemia in adults and uncommon in children. It was found that 46.8% patients suffer from AML, who were under 15 years old, while 19.15% patients their age ranged between 16 - 30 years; 19.15% of patients their age were more than 45 years and 14.9% of patients their age ranged between 31 - 45 years of the total AML patients. AML is more common in males than females. The percentages of females and males were 42.5% and 57.5% respectively representing 1.35 : 1.00 male : female ratio. Two AML patients 5.3% have diploid cells when examined under light microscope. The highest mitotic index was 7.498±1.7 occurred in patients before taking treatment while recorded 6.784±4.5 during treatment and 7.000±2.5 after treatment. Nuclear anomalies such as nucleoplasmic bridges and nuclear buds were observed in AML patients, Micronucleus mean values recorded 0.033±0.018, 0.020±0.00015 and 0.036±0.01for AML patients before, during and after treatment respectively when compared with the control which recorded 0.002±0.00002. Nuclear division index (NDI) means before, during and after treatment of AML patients were 1.658±0.2, 1.000±0.022 and 1.424±0.19 respectively. Significant differences occurred among the three groups when compared with the control group (1.282±0.09). Extracted DNA from 30 AML patients was amplified by PCR to obtain FLT3/ITD gene from exon 11 to intron 12 and ten of them sent for sequencing. Two patients showed larger bands (470 and 460) bp when compared with wild type (330) bp. Among six patients, three displayed point mutations of deletion and substitution while others were normal since no mutations were detected. The percentages of mutation types were substitution 57.78% and deletion 22.2%. The effect of mutations was missense mutation (55.54%), deletion (22.22%) and nonsense (22.22%). It has been concluded from the current work that AML is more common in adult males, patients suffer from AML exhibited high MI, NDI, MN and point mutations are present in such patients including deletion and substitution causing missense, nonsense and deletion.

عزل وتشخيص المبيضات من المرضى المصابين بداء السلاق الفموي في محافظة النجف الاشرف مع دراسة جزيئية لبعض عوامل الضراوة Isolation And Identification of Candida Spp. From Patients With Oral Thrush In Al - Najaf Province And Molecular Study of Some Virulence Factors

اسم المؤلف: ايهاب يحيى جابرالعلياوي

اسم المشرف: فاطمة عبد الحسين التميمي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية العلوم - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: تشمل هذه الدراسة بعض المعايير السريرية لمرضى نقص المناعة (مرض السكري والسرطان وزرع الكلى)، والمصابين بداء المبيضات الفموي والتي تضمنت العمر والجنس. الهدف من هذه الدراسة هو عزل وتشخيص انواع المبيضات. بطرق مختلفة بما في ذلك التشخيص المباشر، والتحضين في الم | The present study was conducted to isolation and identification of Candida spp. Isolated from immunocompromised patients with (Diabetes, Cancer & Kidney transplant) by different identification methods including direct examination, laboratory culture, biochemical tests and molecular method using polymerase chain reaction (PCR) technique and determine the virulence factors phenotypic to Candida spp. and genetic polymorphism in Candida albicans in secreted aspartyl proteinase (SAP2). During the period from October 2013 to February 2014, a total of 140 oral swab were collected from immunocompromised patients with attending to the three center in AL - Sadder Medical City (Oncology center and kidney center and Center for Diabetes and Endocrinology).In AL - Najaf Governorate, the samples were collected as following : - 110 mouth swabs from patients suffering from leukemia, prostate, stomach and bowel cancer, also 10 mouth swabs from kidney transplant patients and 20 mouth swabs from diabetes. The percentage of female to male was as following, female (86) 64(72.7%) infected, 22(42.3%) uninfected and male (54) 30(57.7%) uninfected, 24(27.3%) infected, with age range between (5 - 64) years. In this study, the results show that the incidence of candidiasis in women was higher than the male also the most cases of candidiasis in patients age range between (45 - 54) years. The results of the present study reveal that the prevalence of oral candidiasis from patient with cancer was more than diabetes & kidney transplant patients which was 72(81.18%), 12(13.65%) & 4(4.54%) respectively. When culturing oral samples on the primary isolation media Sabouroud dextrose agar (SDA) and the secondary media on CHROM agar, The results reveal the presence of 88 isolates belong to Candida spp.which include : - 42 (47.68%) belong to Candida albicans, 20 (22.72%) belong to Candida. dubliniensis, 18 (20.5%)belong to Candida krusei, 6 (6.8%) belong to Candida glabrata & 2 (2.3%) belong to Candida parapsilosis. According to this result, Candida albicans was found the most frequently isolated species and the occurrence of the other non - albicans isolates such as Candida dubliniensis and which followed Candida albicans in frequency of the isolates in oral candidiasis. In this study, the ability of C.albicans & non - albicans was tested to produce some virulence factors such as production of enzymes phospholipase, lipase, germ tube, chlamydospore & biofilms when culturing Candida spp. on different media. The results of the culture show that Candida albicans produces all virulence factor included germ tube, chlamydospore, biofilms & phospholipase. Some species share several phenotypic characteristics ability to produce enzymes of phospholipase such as C.krusei while C.dubliniensis produce chlamydospore, when culturing in the Casein agar & produce biofilms, In addition the molecular tests to was used for Candida albicans confirm the ability to secreted aspartyl proteinase (ASP2). This study showed the amplified from the Candida spp. as following : - C.albicans 306 bp, C.glabrata 594 bp, C.parapsliosis 387 bp, C.krusei 651 bp & C.dubliniensis 451 bp.

تقييم الفعالية المضادة للاكسدة والمضادة لحيوية الاطوار المختلفة لذبابة المنزل Musca domestica L. للمستخلص المائي لمخلفات العنب Vitis vinifera L. Evaluation of The Antioxidant And The Activity Against The Viability of Various Stages of The Domestic Fly Musca Domestica L. For The Water Extracts of Grape Pomace Vitis Vinifera L

اسم المؤلف: محمد قاسم بلاسم الخيلاني

اسم المشرف: عبد اللطيف مولان محمد

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: لقد تم تحديد المحتوى الفينولي الكلي للمركبات الفينولية Total phenolic content والفعالية المضادة للاكسدة Antioxidant activity لمخلفات نوعين من العنب (Vitis vinifera) (ديس العنز والشدة السوداء) التي تم الحصول عليها بعد استخلاص عصير العنب وهي القشور والبذور | The total phenolic contents (TPC) and the antioxidant activity of the pomaces and their components (seeds and peels) of two Iraqi grape cultivars (Shadda Black and Des - Alanez) were determined. Four types of solvents were used in the extraction in order to select the best way of extraction of the phenolic compounds. In addition, the insecticidal activity of the water extracts of the pomaces, seeds and peels against different stages of the life cycle of housefly, Musca domestica, was evaluated via studying the effects of the water extracts on egg hatching, the development of the first instar (L1) larvae into pupae and adult flies, and on the viability of the third instar (L3) larvae and pupae. The results of the present study showed that the extracts prepared in the 5% HCl solutions contained the highest (P<0.05) amounts of phenolic compounds followed by those prepared in hdyro - ethanolic solutions (50% with water, v/v), then the extracts prepared in boiling distilled water, while those prepared in normal distilled water (at room temperature) showed the lowest amounts of phenolic compounds. A similar trend of activity was observed for the antioxidant activity via the ability to scavenge the synthetic free radical (2, 2 - diphenyl - 1 - picrylhydrazyl; DPPH). This correlation between the total phenolic contents of the grape pomaces, seeds, and peels and their antioxidant activities clearly indicates that the phenolic compounds are the main contributors for the antioxidant activity. It is important to mention that the TPC of the pomaces, seeds, and peels of the Des - Alanez cultivar were significantly higher (P<0.05 - 0.001) than their counterparts of the Shadda cultivar.The insecticidal activity was evidenced by the ability of the water (boiling) extracts of the pomaces, seeds, and peels of the two cultivars to inhibit the hatching of the housefly eggs at very low concentrations. At 150 microgram (µg)/ml, the crude water extracts from the powdered pomaces, seeds, and peels were able to inhibit completely the hatching of the eggs (100% inhibition) when compared with the eggs in the control group (exposed to water only). The water extracts of the pomaces, seeds, and peels of the two cultivars showed inhibitory effects against the viability of the L3 larvae as evidenced by their ability to paralyze or kill the larvae and this ability increases significantly with increasing the exposure time (P< 0.05) and concentration (P< 0.05). The results also revealed that the ability of extracts of the seeds of the two cultivars to paralyze or kill the L3 larvae was significantly higher (P< 0.5 - 0.001) than the extracts of the pomaces and peels, especially at the higher concentrations.The results of the current study also showed the capacity of the water extracts of pomaces, seeds, and peels of the two cultivars to affect the development of pupae into adult flies and this capacity depends on the exposure time and the by - product and increases significantly (P< 0.05 - 0.001) with increasing the exposure time. Moreover, the ability of the seeds extracts from both cultivars to inhibit the transformation of the pupae into adult flies was significantly higher (P< 0.001) than the ability of the extracts of the pomaces and peels, and at all the exposure times.Concerning the results of the last series of the laboratory experiments which are considered the most important experiments in this study because they simulate field conditions, two treatment placement locations were selected, top of the manure and bottom of manure (under about 5 cm of manure). For top treatment, 50 grams of manure were placed in the plastic cup and then 5% or 10% of the powdered pomaces, seeds or peels were sprinkled on the top of the manure. For bottom treatment, the same amounts of powdered by - products were sprinkled on the bottom of the cup and then 50 g of manure were added to the cup. A similar quantity of untreated manure was used as a control. The results of these experiments showed that the powdered pomaces, seeds, and peels have the ability to inhibit the development of the larvae into pupae and adult flies and this ability depends on the location of the products and the grape cultivar. The results also revealed that the addition of the powdered materials of both cultivars on top of the manure inhibited significantly more (P<0.05 - 0.001) L1 larvae from reaching the pupa and adult stages in comparison with their counterparts which had been added at the bottom of the manure. Contrary to our expectations, the powdered pomaces, seeds and peels of both cultivars showed inhibitory effect against the L1 larvae when added on bottom of the manure which may indicate that the phenolic compounds present in the powdered materials can mix and bind with the ingredients of the powdered by - products and affect the growth and development of the larvae into pupae and adult flies.It can be concluded that the grape pomaces and their ingredients, which are usually thrown in the bin, represent very good sources for phenolic compounds that have the ability to scavenge the free radicals and have insecticidal activity against different stages of the housefly, Musca domestica.

تقييم كفاءة بعض المستخلصات النباتية وعقار الميترونيدازول Metronidazole في حيوية الاميبا الحالة للنسيج Entamoeba histolytica خارج الجسم الحي In vitro مع دراسة جزيئية لتحديد بعض الانواع التابعة لها في محافظة بابل Evaluation of Some Plant Extracts And Metronidazole Drug In Vitro of Entamoeba Histolytica Viability And Molecular Study For Some Species Determination In Babylon Province

اسم المؤلف: سحر عباس حسين المعموري

اسم المشرف: هادي مزعل خضير الربيعي احمد خضير عبيس الحميري

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية للمدة من تشرين الاول 2013 ولغاية تموز 2014 في كلية العلوم للبنات / جامعة بابل, اذ تم الحصول على طفيلي الاميبا Entamoeba من عينات المرضى المصابين والمراجعين لمستشفيات بابل للاطفال والولادة ومستشفى ابن سيف للاطفال والمركز الصحي الحسين | The present study was conducted during the period from October 2013 till July 2014 in the College of Science for women, Babylon University, the Entamoeba stool samples were collected from patients that attending to Babylon Maternity and pediatric hospital, Ebn - Saiff hospital and Abi - Gharaq and Al - Mussaib of primary health center, Babylon province. The parasites were detected by direct smear method, 130 stool samples were collected that contain blood and mucus. The overall percentage incidence of E. histolytica (80.8%). No significance differences of infection rates in males and females and the highest rate of infection was in (1 - 10) year age group for males and females (82.9% and 39.1% respectively) while the lowest infection rate was in (51 year and more) for males and females (33.3 %, 6.7 % and respectively).Three plants extracts (cooled and Boiled) water (Euphorbia helioscobia, Eucalyptus gloubules and Mytrus comminus) evaluated at the culture media (Beef liver infusion media) that grown with E. histolytica. The results revealed the E. helioscobia boiled water extracts the efficient extracts to killed the all parasites in the second day from experiment beginning with 10 mg/ ml concentration and the next efficient plant extracts (boiled water) its E. globules and M. commines was the third day with 5 mg / ml concentration. The Metronidazole showed to killed all parasites in the third day with 1 microliter / ml. and from this present study showed that the successful growth of E. histolytica in culture media for some day and finished (Killed) at the fiveth day from experimental beginning.Isolation and identification of some terpenoid compounds were detecting in the present study belong E. helioscobia plant extracts (Camphene, ???pinene, Euphornin, ?? caryophyllene, Myrecene and ??humulene) by (HPLC).Molecular study was conducted to determined three species of Entamoeba by using Polymerase chain reaction for ten stool samples that primarily detected with Amoebic dysentery by direct smear method and the following results for molecular study, the highest overall percentages its E. dispar with (7/10) mean 70% whereas the E. histolytica and E. moshkoviskii its (6/10) mean 60 % for each one, as well as existence the double infection or tertian infection with these species for Entamoeba.

التشخيص الجزيئي لبعض انواع الفطر Trichoderma وتقييم فعاليتها ضد الديدان الثعبانية لعقد الجذور Meloidogyne javanica في محافظة واسط Molecular Diagnosis of Some Species of Trichoderma And Evaluation Their Activity Against Root - Knot Nematode Meliodogyne Javanica In Wasit Province

اسم المؤلف: حمزة عباس ياسر

اسم المشرف: محمد جبير حناوي هادي مهدي عبود

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة واسط - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: واسط

الصفحات الاولى:

المستخلص: نفذت هذه الدراسة لعزل وتشخيص الانواع المستوطنة للفطرspp Trichoderma في محافظة واسط وتقييم فاعليتها ضد نيماتود العقد الجذرية Meloidogyne javanica (Treub) في مختبر الفطريات في قسم علوم الحياة - كلية العلوم - جامعة واسط فضلا عن دراسة تاثير هذه الانواع في بعض | This study has been conducted to isolate and identify some nation species of the fungus Trichoderma spp in wasit province and evaluate the activity of them against Root Knot nematode Meloidogyne javanica (Treub) in the laboratory of Biodepartment - College of science - University of wasit, and study the effect of these species in growth criteria of tomato plant in greenhouses of Ministry of Technology and Science, and diagnosed by PCR technology. Soil samples had been collected from soil and plant roots (solanaceae, cucurbitacece) infected with Meloidogyne javanica in fields and greenhouses in eight locations in wasit province (Kut center, Alahrar, Alhay, Alnuamania, , Badra, algardhia, sheikh saad and Aldijaily) for the period from 5/11/2013 to 22/12/2013.The results had been revealed that there are 15 isolates of 25 isolates contain Trichoderma spp, by the purification we got 7 a local isolates of the fungus Trichoderma belong to four species of Trichoderma which were T.harzianum، T.hamatum، Trichoderma sp1 and Trichoderma sp2.Results showed All tested isolate high efficiency in parasitism the eggs of nematode and the reduction of the percentage of hatching eggs and the more effective isolate was the T. harzianum3 (T6) from sheikh saad location comparing with the other tested isolates and the percent of parasitism was 97%, and significant differences with all isolates and the results showed a clear reduction in the number of larvae emerging comparing with the control.The results showed that the isolates T.hamatum1 (T1), T.harzianum2 (T3)، T.hamatum2(T7) exist high percent of parasitism which were 92.2% , 90.8% , 90.4% respectively It was not a significant difference between them, while the isolates T.harzianum1 (T2)، Trichoderma sp1 (T4)، Trichoderma sp2 (T5) were 80.4%, 77.5%, 84.6% respectively.The result also showed that the filtrate of isolates (100%, 50%, 25%) had good effect on egg hatching (destruction) comparing with the control and the more effective concentration was 100% Compared to other concentrations and the filtrate of isolate T. harzianum3(T6) was more effective comparing with other filtrate of isolates and the mortality of eggs were 96.7%، 75.5، % 56.3% at the concentration 100%, 50%, 25% respectively The Trichoderma sp2 (T5) was least effective isolate compared with other isolates, as the proportion of parasitism was 76.4%، 59.7%، 38.6% at the concentration 100%, 50%, 25% respectively. With regard to the effect of fungus in tomato plant growth criteria the results also showed that the isolate T. harzianum3 (T6) enhanced the growth of the length of stem and root of tomato plant growing in greenhouses and the lengths of stem and root treatment with T. harzianum3 (T6) were (19.7, 21.3) cm respectively Followed by the the control which were (16.1، 15.4) cm respectively while the lengths of stem and root of treatment plants with the fungus and nematode together were(14.4، 11.1) cm respectively and least length of stem and root of treatment plants with nematode only were (9.6، 6.2) cm respectively.The results of the polymerase chain reaction (PCR) revealed a Positive results by using SCAR primer in this technique to two isolate of fungus Trichoderma (T. harzianum and T. hamatum) through success in the amplification process to 7 isolate of the fungus as given expected band (837 base pairs) which back to the species T.harzianum, while the species T.hamatum the size of band was (450 base pairs), the recording of two species using this technique is the first in Wasit province and Iraq.

تقييم الواقع البيئي لمعمل بابل /2 للبطاريات Assessment Study For The Environmental Situation of Babylon\2 Batteries Plant

اسم المؤلف: منال مالك سعدون الموسوي

اسم المشرف: سهير ازهر الكاظمي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: البيئة

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: In this study was investigated the quantity and quality of industrial water from (Babil/2 batteries plant in Baghdad) and estimate the amount of air pollutants for some factory sections and estimate the amount of solid waste resulting from the industry and study the impact of these pollutants on the environment and public health.The first target of this study is investigated the physical and chemical characteristics of the discharged water from sections shipping and ficus where the sample water collocated was two samples per month for 6 month was taking during period from Novembers 2013 to May 2014. Some measurements were conducted laboratory and others on site, the result showing the following values of the variables rates for the shipping and ficus sections before treatment and respectivelyResults of the study showed that there is a significant increase inThe concentrations in sulfate and TDS, heavy metal When reached after treatment for the same variables and respectivelypH 9.9, EC 1524.9 ?s/cm, TSS 166.4mg/l, TDS 611.7mg/l, Cl - 64.46mg/l, (SO4)+2 486.8mg/l, BOD 49.2mg/l, COD 220.4mg/l, Pb+2 0.55mg/l, Zn+2 0.09mg/l, Cd+2 0.01mg/lpH 8.2, EC 1191.6?s/cm, TSS 68.1mg/l, TDS 927.6mg/l, Cl - 76.95mg/l (SO4)+2, 722.6mg/l, BOD 4.4mg/l, COD 29.2mg/l, Pb+2 0.04mg/l, Zn+2 0.03mg/l, Cd+2 0.01mg/lFrom the results of the study showed that there is a no significant increase in the concentrations in sulfate and TDS, but increases slowly. In the water of charging department reached the concentration of same variables and respectivelyThe second aims of this study a steamed the concentration and rate of air pollution (Total Suspended Particles, heavy metal, Oxides and gases) collected the sample of outstanding minutes analysis made to know and identify the concentration of heavy metal for same factory section and compared those with local and global determinants also the concentration of air emitted was measured.Results of the study showed that there is a significant increase in the concentrations of the outstanding minutes compared to the allowable limits as it reached the highest concentration in the Department of Shipping 3313.46?g/m3. The lowest concentration was in the section of the Aggregation 0.8155?g/m3. Where the concentration of heavy metals, especially lead element exceeded the permissible limits as it reached the highest concentration in Ficus section 3.53?g/m3 the lowest concentration in the assembly department which is 0.36 ?g/m3 concentration of CO gas in the department of Ficus, Shipping, assembly, queries reachedpH 1.6, EC 12058?s/cm, TSS 148.3mg/l, TDS 10122mg/l, Cl - 76mg/l(SO4) +2 3838mg/l, BOD 23.55mg/l, COD 203.5mg/l, Pb+20.55mg/l Zn+20.46mg/l, Cd+2 0.022mg/lrespectively.(21.7, 3.9, 2.44, 2.84?g/m3), the concentration of CO2 was 231.8, 187.6, 219.4, 225.4?g/m3.In the department of Ficus, Shipping, assembly, respectively queries either NO2 was 0.12?g/m3 in sections of Ficus, Shipping and queries, 0.16?g/m3 in the assembly department. The H2S appeared only in sections Ficus, Shipping with concentration 0.02?g/m3, 0.18?g/m3 respectively, either gas SO2 only appeared in the shipping department and was only 0.01 ?g/m3.The last part of these study was to calculate the amount of solid waste for a period of study in terms of total solid waste generated by the plant was 37.004 tons for eight months and included this waste pastes lead and lead plates and scrap processing units either the amount of recycling of batteries that are broken in the foundry of lead in Khan Dhari amounted to 354.832 tons for eight months, and the output of 181.1 tons. In this study we foxed on the problems in these factory that effected the process of industry and we found liquid wastes in the factory is not efficient in reducing the concentrations of all the variables treatment and gaseous emissions generated in the factory, there are no have any significant treatment of solid waste recycled and are utilized as it emerged that the Babil / 2 battery plant on a large degree of pollution sulfate and TDS with regard to liquid waste. The minutes and the outstanding lead with respect to gaseous pollutants to the current study that recommended a set of recommendations that would work to eliminate or minimize the causes of pollution and thus access to good results.

تاثير الملوحة في نمو ونشاط بكتريا الازوتوباكتر واثرهما في نمو نبات الحنطة Triticum aestivum L. The Impact of Salinity On The Growth And Activity of Azotobacter. Spp And Their It Imfuces On The Growth of The Plant Wheat (Triticum Aestivum L.)

اسم المؤلف: اية خالد كريم

اسم المشرف: فارس محمد سهيل

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: الخلاصة اشتملت الدراسة تنفيذ خمس تجارب, فضلا عن عزل بكتريا الـ Azotobacter وتنقيتها وتوصيفها وذلك من خلال جمع 16 عينة تربة من رايزوسفير محاصيل عدة من مناطق مختلفة من محافظة ديالى.كانت اربع تجارب مختبرية نفذت بهدف قياس اقل تركيز مثبط MIC من الـ NaCl في نم | The study imvolved implementation of five experiments, as well as the isolation, purification and classification of the bacteria (Azotobacter) and through the collection (16) soil samples from Rhaizosphre several crops from different regions of the province of Diyala. The four laboratory experiments carried out to measure less inhibitory concentration MIC in the growth and the number of bacteria Azotobacter and fixation of atmospheric nitrogen and produce GA3 from bacteria. Fifth experiment pot experiment to study the effect of two types of bacteria Azotobacter and interference with water drainage in the growth and yield plant wheat (Triticum aestevium L.) type (Aba 99).Isolate and diagnose the bacteria Azotobacter : The screening showed and diagnostic results that 11 isolation is a subsidiary of the type A.chroococcum, and 5 isolates belonging to the sensitization A.vinelandii.The isolates (A10, A2, A6, A8) was chosen from A.chroococcum and (A9, A5, A16, A14) was chosen from A.vinelandii was chosen as local isolate used in laboratory experiments based on the measurement of the soil salinity isolated ones, and selected isolates A10, A8, A5, A14 local isolate used as biofertility to the experience of pots because of their ability to nitrogen fixation by measuring the amount of nitrogen fixation in the third experiments. Laboratory experiments : The first and second experiment : Two trials were conducted Laboratory by using randomized complete block design (RCBD), each experiment included (120) experimental unit for each lap. Resulted from (8) isolates bacterial and five Trakizmn salt NaCl (0, 1, 3, 5, 7)% and (3) replicates, and incubated for three periods lap (1.3, 6) days to calculate the (MIC) in the growth and cell numbers bacterial. The results showed that the number of bacteria cells A.vinelandii rate increase all isolates A.chroococcum regardless of the concentrations of NaCl and extended incubation. Although isolates (A16, A14) isolated from soil salty surpassed isolates (A9, A5) isolated from soil is salty, and isolates (A8, A6) A.chroococcum of bacteria isolated from soil salty surpassed isolates (A10, A2) isolated from non - saline soil. The highest values of the numbers of bacteria are isolates A8, A16, amounting to (11.13, 11.0) * 104.cfu g - 1 dry soil. The increase in the concentration of NaCl led to a decrease significantly in the growth and the number of bacteria Azotobacter cells as the decline amounted to 17.13%, 45.52%, 59.50%, 76.99% at concentrations of 1%, 3%, 5%, 7%, respectively, and recorded the lowest numbers in the values of focus 7%, reaching 3.80 * 104. Cfu g - 1 dry soil. The growth and numbers of bacteria Azotobacter increased treatment effect when increasing the extended cuddling and all concentrations of NaCl. In a period of one lap on all isolates were grown in 1% NaCl concentration of the MIC for all isolates focus is 1%, while the two lap 3 days, all isolates growing focus recorded (3%, 5%) excluding A16 isolation of bacteria A.vinelandii was able to focus on growth until 7%, so the damper the MIC for this isolation focus is to concentrate 7%, while the rest of the isolates the MIC have 5%, which indicates that this isolation is more resistant to salinity of the isolates.The third experiment : The results showed that the rate of nitrogen fixation bacteria Azotobacter between (0.008 - 0.04%), but an increase in local concentrations led to a decline in moral decline as nitrogen fixation rate of 50.0%, 77.5%, 95.0%, 98.0% at concentrations of 1% salinity, 3%, 5%, 7% respectively.All isolated of bacteria Azotobacter can nitrogen fixation in the all salinity level. although A.chroococcum isolates isolated from non - saline soil surpassed isolates isolated from soil salty and all levels of salinity. Fourth experiment : A laboratory Conducted experiment by using a randomized complete block design (RCBD), included the experiment (12) experimental unit. Resulted from (4) bacterial isolates and three concentration of salt NaCl (0, 3, 5)% to calculate the MIC in the production of bacteria gibbereline Azotobacter. The results showed that the production rate of bacteria gibberline Azotobacter and all isolates ranged from 0.996 - 3.6 mg. L - 1, regardless of the concentration of added NaCl. And increasing salinity levels have led to a reduction in the production of Aljprlin, recorded A8 isolation from A.chroococcum isolated from soil salty higher values in the production of Aljprlin and all levels of salinity Experiemet of pots : The experiment was Carried out in the College of Agriculture, University of Diyala in the soil of a fusion sand using a design full sectors randomized (RCBD) in the winter season of the year (2013 - 2014). The experiment included on (60) experimental unit, resulting from the overlap between the four isolates bacterial treatment compared without the addition of bacterial vaccine and four concentrations of water drainage. The results showed that the addition of the vaccine bacterial gave a significant increase in plant height, dry weights of grouped shoot and root, leaf area, chlorophyll content, holds grains, protein and the concentration of elements of N and decrease the concentration of Na elements, Cl and proline.The addition of the vaccine bacterial and all isolates used led to a significant increase in plant height, dry weights of grouped shoot and root, leaf area, chlorophyll content, holds grains, protein and the concentration of elements of N, and decrease the concentration of Na elements, Cl and the preparation of bacteria Azotobacter in the soil compared to non - bacterial vaccine in addition to all the concentrations of water drainage. The isolates were isolated from soil salty gave an increase in traits higher than isolates isolated from non - saline soil.

دراسة نسجية مقارنة للامعاء بين طائر الحمام الضاحك وطائر الرفراف Comparative Histological Study of Intestine Between Laughing Dove And Kingfisher

اسم المؤلف: علي نديم جواد الشباني

اسم المشرف: عدنان وحيد البديري

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: هما طائر الحمام الضاحك او فاختة النخيل Laughing dove من الطيور اكلات الحبوب وطائر الرفراف Kingfisher من الطيور اكلات اللحوم اعتمادا على طبيعة الغذاء. تضمنت الدراسة فحص مظهري ونسجي للامعاء لكلا النوعين في ثلاثين طائرا بالغا تتراوح اوزانهم بين 96.6 ± 0.87 | The study was designed for the purpose of identifying the tissues structure in the intestines of two Iraqi wild birds, Laughing dove, granivores birds and Kingfisher of birds carnivores birds depending on the nature of the food. The study included morphological and histological of intestines of both types in thirty adult birds their weights ranging between 96.6 ± 0.87 grams in the laughing and 101.8 ± 0.49 grams of Kingfisher. These birds were obtained by caught from different areas in AL - Qadisiya province regardless of their sex and then divided into two groups, every group included fifteen birds represent the order of studied birds. Five birds for the purpose of studying the morphological structure and another ten for the purpose of examining the histological structure, from each group. Birds were Seduced and opened by the abdominal area carefully. The intestines of the birds were visually described, both the small and the large intestine. Both birds intestines were separated from their bodies.Then, the weights of birds were accounted. After that, the ratio of birds intestines was recorded according to birds bodies. The results of morphological structure have showed that there are similarities in the parts and position of intestines in both studied birds.Also, it has been showed that the ratio of weight of both small and large intestines to the body was higher in kingfisher bird 2.23% and 0.42% compared with that ratio in laughing dove 1.94% and 0.52%. The intestine of laughing dove was longer than that of kingfisher.The duodenum forms single loop that encircles pancreas in both birds. There is no diverticuli vitelini that separates jejunum and ileum. It has been found that there were couple of cecaea in laughing dove bird and not found in white breasted kingfisher. The results of histological study has showed that the wall of intestine in both birds consists of four main layer different in their thickness which are mucosa layer, submucosa layer, muscularis layer and serosa layer.The mucosa layer consists of simple epithelium, lamina propria and muscularis mucosae layer.The three parts of intestine duodenum, jejunum, ileum have similarity in their histological structure in that the mucosa layer modified into a lot of villi which were longer and thicker and contain more branches in its base parts in kingfisher compared with that of laughing dove birds which was shorter and thinner, The villi were covered with a lot of columnar cells and brush border that have goblet cells which their number increase toward the of digestive canal. It has been noted that there was differences in layer thickness that form intestine wall.The mucosa layer was thinner compared with other layer. The villi of ceacae were short and flat, the villi of rectum were short in both birds.The cloaca villi were longer and clearer of laughing dove that of white breasted kingfisher. It has been concluded that the difference of food type that the birds have can have clear effect in both morphological and histological structure of intestine ; namely, the thickness of layers that forms their walls.

دراسة تاثير المستخلص المائي لنبات الحناء Lawsonia inermis كمضاد للاكسدة في ذكور الجرذان البيض المعرضة للاجهاد التاكسدي A Study Effect of Henna Aqueous Extract Lawsonia Inermis As Antioxidant in Exposed Oxidative Stress White Male Rats

اسم المؤلف: سعدية جمال قادر السامرائي

اسم المشرف: صالح محمد رحيم العبيدي منيف صعب احمد ساجت الجنابي

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: صممت هذه الدراسة لمعرفة تاثير المستخلص المائي لنبات الحناء Lawsonia inermis كمضاد اكسدة في ذكور الجرذان البيض المعرضة للاجهاد التاكسدي المستحدث ببيروكسيد الهيدروجين مع ماء الشرب طيلة مدة التجربة البالغة 30 يوما، بالاضافة الى فيتامين C المعروف كمضاد قوي لل | The study was examined the effect of aqueous extract of the Lawsonia inermis antioxidant in Albino male rats which exposed to oxidative stress induced hydrogen peroxide with drinking water for the duration of the experiment of (30 days), compared with vitamin C, known as strong anti - oxidant.1. The results showed that hydrogen peroxide led to a high moral (p <0.05) in the level of Glucose and Cholesterol, Triglycerides (TG) and low lipoproteins - lying density cholesterol (LDL - C) and lipoproteins low - lying density cholesterol is very (VLDL - C) and evidence Atherosclerosis, phospholipids and Malondialdehyde (MDA) peroxynitrite radical (ONOO - ) , Urea and Total count of white blood cells (WBCs), as well as significant decrease at the level (p <0.05) in the level of high - density lipoproteins cholesterol (HDL - C) and the level of, Glutathione (GSH), and Ceruloplasmin (CP), Uric acid, Albumin, serum Creatinine in the blood of Albino male rats exposed to oxidative stress.2. Resulted in treatment of male rats, eggs exposed to stress oxidative vitamin C (concentration of 250 mg \ kg of body weight) to a significant decrease (P ? 0.05) compared with the group treated with H2O2 only in the total count of white blood cells and the level of glucose, cholesterol, Triglerids and lipoproteins and low density cholesterol and very low - lying density of cholesterol and signs Atherosclerosis and Phospholipids, Urea, and the Malondayaldehid Peroxynitrite radical, while the moral high concentrations in high - density lipoprotein cholesterol, uric acid, Albumin, Creatinine, and, Glutathione and Ceruloplasmin.3. As well the treatment of animals exposed to stress oxidative Palmstkhals water plant henna to a significant decrease compared with the group treated with H2O2 only in the total number of white blood cells(WBCs) and concentrations of glucose, cholesterol, Triglycerides (TG), lipoprotein and low density lipoprotein choesterol LDL - C, and low density is very VLDL - C, phospholipids, levels of evidence Altasd, Urea, and the Malondayaldehid MDA and Peroxynitrite radical, (ONOO - ) while the moral high concentrations in high - density lipoprotein choesterol, HDL - C, Uric acid, Albumin, and, Glutathione and Ceruloplasmin.4. That the treatment of Albino male rats group water plant henna led to a significant decrease in the concentration of Malondayaldehid Peroxynitrite radical (ONOO - ) compared with the control group, while did not show significant difference (P> 0.05) in the concentrations of Glucose, total cholesterol, Triglycerides (TG), lipoprotein, high - density cholesterol lipoprotein HDL - C, and low density cholesterol lipoprotein LDL - C, and low density cholesterol is very VLDL - C, phospholipids, Atherogenic indices levels I, II and III, Uric acid, Creatinine, Albumin, Ceruloplasmin, Urea, peroxynitrite radical and total (WBCs) number of white blood cells compared with control group. It is clear from the current study that aqueous extract of henna, vitamin C role of great importance to reduce the emergence and development of atherosclerosis induced proxaid hydrogen as anti - oxidation which removal of free radicals, and contain the extracted compounds in particular have an impact on physiological and biochemical events occurring in the body and without side effects makes it possible to make use of the prevention and treatment of many different conditions, which may reflect the preventive role of this extract against free radicals.

الكشف عن بعض عوامل البيئة الداخلية للخلايا السرطانية للنساء العراقيات المصابات بسرطان الثدي Detection of Some Microenvironment Factors In Tissue Samples of Iraqi Women With Breast Cancer

اسم المؤلف: فاطمة سمير عبد الرزاق الزبيدي

اسم المشرف: اخلاص مشرف عيدان

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - المناعة

الدرجة: ماجستير

الجامعة: جامعة بغداد - كلية العلوم للبنات

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: Breast cancer (BC) is the most common cause of cancer - related deaths in women. It continues to rank as one of the top killer of women. This cancer increased in frequency in the last years in Iraq. The aim of this study was to shed light on the immunohistochemical for some factors that could be affect on development of microenvironment in breast cancer of Iraqi patients. And these factors include CD133 as a marker for breast cancer stem cells (BCSs), and also studying tumor necrosis factor alpha (TNF - ?) and transforming growth factor beta (TGF - ?). 53 samples Formalin - fixed, paraffin - embedded (FFPE) wax block appeared their ages were range from 29 - 70 year with a mean age of 48.45 years. 32 patients with breast carcinoma and 21 patients with benign breast lesions were included in this study for comparison. The results of this study show that the CD133 positive expression was found in (56.2%) of Iraqi breast cancer cases. Also the result of this study show that (62.5%) positive expression from both (TNF - ? &TGF - ?) of breast cancer cases, compared with sample of benign breast lesion. The results show (52.3%) positive expression of TNF - ? and (28.57%) positive expression of TGF - ? of samples with benign breast lesion, there is a significant different between studied samples, compared with (19.05%) samples positive expression of CD133 of sample with benign breast lesion so there is a significant different between the samples with breast cancer and benign breast lesion. Also the results show there is a positive relationship correlation between (TGF - ?) expression and (TNF - ?) expression, while there were no relationship correlation between (TGF - ?) and CD133 and no relationship correlation between TNF - ? and CD133. The results show there is a positive correlation between the grade and breast cancer with the three different expressions of marker but in different strongest correlations between (TNF - ?) and (TGF - ?) with the graded but this correlation becomes weak with CD133 marker the value of significant. According to the relationship in breast cancer case in this study between the studied markers and stage of case it shows (TNF - ?) has a strong positive correlation while the correlation appear week between the stage of this studies case and end each of TGF - ? and CD133. For this we concluded from the results there high expression of CD133 and TNF - ? indicators and TGF - ?. CD133 could use in diagnosis of the cancer cell and the high expression of TNF - ? & TGF - ? indicate that these factor play important roles in tumor microenvironment metastasis. And the strong correlation between the expression of these markers with grade and stage of breast cancer

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

اسم المؤلف: كرم رياض حسن الجراح

اسم المشرف: رسمية عبد ابو ريشة

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).

انشاء وتوصيف لخط سرطان الثدي الخلوي العراقي Establishment And Characterization of Iraqi Breast Cancer Cell Line

اسم المؤلف: مرتضى عادل الشامي

اسم المشرف: محفوظة عباس عمران احمد مجيد الشمري

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: التقنيات الاحيائية

الدرجة: ماجستير

الجامعة: جامعة بغداد - كلية العلوم - قسم التقانة الاحيائية

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

التحري عن عوامل الضراوة لبكتيريا اشيريشيا القولون المعزولة من المرضى المصابين بالتهاب المجاري البولية وقياس نمط بعض الحركيات الخلوية لديهم Detection of Virulence Factors of Escherichia Coli Bacteria Which Isolated From Patient With Urinary Tract Infection And Measuring Some Cytokines In Them

اسم المؤلف: رواء ماجد محمد البو صالح

اسم المشرف: ميثم غالي يوسف

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية العلوم - قسم علوم الحياة

اللغة: العربية

النص الكامل من المصدر: https://qu.edu.iq/repository/wp-content/uploads/2017/04/%D8%B1%D9%88%D8%A7%D8%A1-%D8%A7%D9%84%D8%A8%D9%88%D8%B5%D8%A7%D9%84%D8%AD.pdf

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: تضمنت هذه الدراسة جمع 100 عينة من اعمار مختلفة للمرضى المصابين بالتهاب المجاري البولية الذين راجعوا مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية , خلال المدة من تشرين الثاني 2012 الى نيسان 2013 للتحري عن بكتيريا Escherichia coli ا | groups of patient with pyelonephritis, whose visited Al - Dewaniya teaching and Woman s and children hospital in Al - Dewaniya city during the period from November 2012 to April 2013 for detection the isolates of Escherichia coli. The results showed that 56% of bacterial isolates were E.coli and 44% isolates belonged to others bacterial types. These results indicated that the females were more infected 67.86%(38\56) with pyelonephritis than the males 32.41(18\56). Always our result were pointed that the age of 30 - 39 old years have beeninfected with pyelonephritis and present the high percentage infection 30.36% comparison with others age groups (1 - 9, 10 - 19, 20 - 29, 30 - 39, 40 - 49, 50 - 59, 60 - 69, 70 - 79) , with percentage of (1.79%, 7.14%, 14.29%, 30.36%, 19.64%, 10.71%, 12.50%, 3.57%) respectively. The resistance of E.coli isolates to the four generation of Cephalosporin antibiotics were showed the following state : - The first generation of Cephalosporin antibiotic were indicated to Cephalothin 76.6%, Cefazolin 71.4%, Cephlexin 69.7% and Cephadroxil 66.1%. - The second generation of Cephalosporin antibiotic were indicated to Cefaclor 59.0%, Cefonicid 60.8%, Cefprozil 50.0%, Cefoxitin 64.2% and Cefmetazole 55.3%. - The third generation of Cephalosporin antibiotic were indicated to Ceftriaxone 32.2%, Cefotaxim 35.7%, Ceftazidim 37.5%, Cefixim 42.9%, Cefdinir 35.7% and Ceftizoxim 30.4%. - The fourth generation of Cephalosporin antibiotic were consisting of one antibiotic only like Cefepime with percentage of 39.3% The results concerned the virulence factors genes showed that the gene irp2 witch responsible for taking the iron from the blood, whereas the gene pap responsible for the production of P - type Pilli, afa gene responsible for the production fimbriae, iha gene responsible for production of capsule and the gene tst responsible for toxic shock. The result of PCR for the E.coli DNA showed that all the thirty isolates of this bacteria contain the gene irp2, while the others genes pap, afa, hly, and iha were presented the following percentage 36.6%, 30.0%, 96.6% and 10.0%respectively, whereas the gene tst didn’t recorded any isolates concerting. So in this study was to measure immune factors related to the urinary tract infection is in general and pyelonephritis in specially, has been measure some of cytokines which (Interleukin - 8, Interleukin - 6, Tumor necrosis factor - ?), they found increase in these factors in sera of patients with pyelonephritis compared to healthy persons.

عزل وتشخيص بعض فطريات الاذن الخارجيه واختبار قدرة الفطر Aspergillusعلى افراز بعض انزيمات عوامل الضراوة Isolation And Identification of Some Fungi of Otitis Externa And Testing The Ability of Aspergillus Spp. To Produce Some of Virulence Factors Enzymes

اسم المؤلف: وفاق احمد محمود

اسم المشرف: سميرة كاظم حميد ياسر لفتة حسون

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: جمعت (113) عينه سريريه (مسحة اذن Ear swab) لمرضى التهاب قناة الاذن الخارجيه الفطري Otomycosis من كلا الجنسين والذين راجعوا استشارية الانف والاذن والحنجره في مستشفى الصدر التعليمي والعيادات الخاصه للاطباء الاختصاص / محافظة النجف الاشرف للمده من تشرين الثان | This study included 113 ear swabs from patients with otomycosis, males and females, visited the otolaryngology consultation room at Al - Sader Medical City in An - Najaf / Iraq in addition to the private otolaryngology clinics of some of ear, nose and throat specialists, during the period from October 2012 to March 2013, the study aimed to isolate and diagnose the causative opportunistic mycotic infections of the ear and to test sensitivity of these organisms towards antimycotic agents in addition to identifying some enzymes of the most fungal reluctant type phenotypically and molecularly. (103) positive swabs were obtained from total samples including "Cephalosporium, Candida spp, Aspergillus spp, Neurospora and Trichosporium beigelii.The Precentage of isolated fungi was (85.43)% for Aspergillus, (10.67)% for Candida, while other species constituted totally (3.88)%. The study show that the highest percentage of isolated fungi was from males as it was(51.4)% while in females was (48.6)%. Patients' ages were found to be between (11 - 80) years and the age group (21 - 30) years was the most frequent Category in (30.3)% and the Category (71 - 80) years was affected only in (0.8)%. The highest percentage of insulation fungi during the period of sample collection was in February which constitute (30.9)% and the lowest percentage was in March reaching only (14.1)%.The highest ratios to isolate the fungus was recorded among housewives and unemployed males and it was (44.2%), while the lowest rates of insulation when the students reached (15.9%).The cases of otitis media excelled on the rest of the pathological cases accompaniments of Otomycosis as the proportion of insulation was (19.4%), followed by disease of blood pressure which their percentage was (5.3%), while the rest of the pathological cases did not have significant influence in insulation, for more ear side affected the left ear was affected in(51.8)% and the right was affected in (48.2) %. The study showed that there is inhibitory effect of (flucanazole, Nystatin, Clotrimazole, Miconazole, Itraconazole and Ketoconozole) against Aspergillus spp (A.niger, A.flavus and A.terreus). All antifungals were effective and their inhibitory ratio was (100) %, except for the antifungal (Fluconazole ) which found to have a lower inhibitory effect (10) % against the species A.flavus only. Testing the phospholipase activity for aspergillus species, A.flavus and A.niger showed phospholipase activity in two degrees (++, +++), but A.terreus shoed only (++). Searching the gene alp " which is encoded for the production of alkaline protease " showed that all the Aspegillus species produces the gene with amplification results of (100)% for the band supposed to appear at the molecular weight of (747) pb

عزل وتشخيص الفطريات المصاحبة لاخماج الجيوب الانفية وحساسيتها لبعض المضادات الفطرية Isolation And Identification of Sinusitis Infections Borne Fungi And Its’ Sensivity To Antifungals

اسم المؤلف: رواق حيدر عبد النبي المحنة

اسم المشرف: ماجد كاظم عبود الشبلي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - الفطريات

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: جمعت ((97 مسحة انفية من المرضى المصابين باخماج الجيوب الانفية, ولكلا الجنسين وباعمار مختلفة والذين راجعوا العيادات الخاصة بالانف والاذن والحنجرة Ear Nasal Throat في مدينة الديوانية للفترة من تشرين الثاني 2012 الى نيسان 2013, لغرض عزل وتشخيص الفطريات المسب | A total of 97 nasal swab were collected from patients with sinusitis, for both sexes with different ages who attended private clinics of Ear Nasal Throat in the city of AL - Diwaniya, for the period from November 2012to April 2013, for the purpose of isolating and diagnosing fungi that cause inflammation of the sinuses and test their sensitivity toward some antifungal as well as the study of the effect of some factors on the infictions rats, which include age, gender, housing, and professions and relationship of chronic diseases. Specimen were cultivated on culture media SDA to determine the types of fungi responsible the infection, it is found that the samples gave positive growth were about 36 at a rate of 37.11 %, while the samples which gave a negative growth were about 61 at a rate of 62.88 % Note that some samples give more than one fungal type so amounted to 44 the number of fungal infections and 45.36 %.The isolated fungi included and their rates Aspergillus flavus13.63%, Aspergillus niger 13.63 % , Aspergillus terreus 4.54 % , Pencillium sp 18.18 % , Blastomyces dermatitidis and Cladosporium sp 11.36 % of each one , Alternaria sp and Trichophyton sp 4.54 % of eachone , Troulopis sp , Mucor sp , Rhizopus sp, Paecilomyces sp, Rhodotorula sp, Ulocladium sp, Geotrichium sp and Bipolaris sp 2.27% of each one. The presnt study showed that females cases are more than males cases, where the percentage of female infection about 63.63% but male infection rate of about 36.36%, patients ranged in age between 11 - 76 years and found that the infection was more frequent among age groups 20 - 29 years 29.54% and less frequent among age groups 19 - 11 years 9.09 %. About the injury season shows that increase in the cold months of the year and less in the warm months, as it recorded the highest rate of infection during November at a rate of 43.18 %, while there have been no injury during April, as for Relationship of injury with housing areas of patients has been shown that the highest proportion of the injury people registered from urban areas 52.27 % compared to residents of rural areas 47. 72 %. The role of the professions in the incidence of injury has been shown that the highest recorded injury among housewives40.9%and the least among the unemployed 2.27%, while the relationship of chronic diseases has revealed the presence of 15 people suffering from chronic diseases and fungal infections arate of 15.45 %, the more chronic injuries were pressure disease, where they formed an injury rate of 40%.Results showed the inhibitory effect for antifungal Itraconazole, Ketoconazole, Econazole, Clotrimazole, Nystatin, Amphotericin B against 12 type of fungus Pencillium, Blastomyces dermatitidis, Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Cladosporium sp, Alternaria sp, Troulopsis sp, Mucor sp, Ulocladium sp, Geotrichum sp , Bipolaris sp , the antifungal Econazole was the most antifungal effective against isolated fungi and diameters of inhibition rate 30, 38, 20, 33, 30, 40, 35, 39, 27, 31, 19, 44? m of fungal species respectively, while the antifungal Amphotericin B show less effect against isolated fungi at arate of inhibition diameters 14, 19, 10, 10, 8, 12, 26, 18, 17, 22, 18, 21? m, respectively.As for the values of the MIC and MFC for Ketoconazole and Itraconazole for the fungi Aspergillus niger, Aspergillus flavus , Geotrichum sp found that antifungal Ketoconazole owned less MIC against all fungi, as it amounted to 0.24 Mg /ml of each one, while the MF Cagainst Aspergillus niger and Geotrichum sp was 7.8Mg /ml against Aspergillus flavus was 0.48 Mg /ml, while the antifungal - Itraconazole owned less MIC was 31.25 Mg /ml against all fungi, while the MFC was 62.5 Mg /ml against all isolates.The presnt study showed That fungal infections is one of the important causes of sinusitis infections and fungus Aspergillus spp is the most common.

دراسة مقارنة للتاثير السمي للزنبور الاحمر Vespa Orientalis L والزنبور الاصفر Polistes Olivaceaus (De Geer) (Hymenoptera : Vespidae على تحلل كريات الدم الحمراء والتخثر لدى الانسان Poison Effects of Red Wasp Vespa Orientalis L. And Yellow Wasp Polistes Olivaceaus (De Geer) (Hymenoptera : Vespidae) On The Decomposition of Red Blood Cells And Coagulation In Humans

اسم المؤلف: شهد فلاح عباس

اسم المشرف: سعدون ابراھیم اسماعیل محمد عبد الرزاق الصوفي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان - الحشرات

الدرجة: ماجستير

الجامعة: الجامعة المستنصرية - كلية التربية الاساسية

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: To study the impact of toxic of the the Red Wasp Vespa orientalis L. and yellow wasp Polistes olivaceaus (De Geer) from family (Vespidae) the decomposition of red blood cells and clotting in humans. Were collected 508 insect Red wasp was where the number of workes 390 insect the yellow wasp gathered 539 insect where the number of worker 418 insect, and the diagnosis was confirmed based on the taxonomic keys and professors speclalized in the natural history museum and then extracting machine stinging for poisonWasp red and yellow toxin were purified by gel filtration throw Sephacryl S - 200 were obtained on three peaks of proteins, and when estimated the activity of lysis and clotting for peaks separate P1, P2 and P3 for wasp red toxin, the lysis activity of separate P1 was 98% of the poison origin activity and the clotting time was 47.3 minutes, while the lysis activity of separate P2 was 62% of the poison origin activity and the clotting time was 27.53 minutes, and the lysis activity of separate P3 was 29% of the poison origin activity and the clotting time was 13.67 minutes, so P1 separate from Wasp red poison was selected to purification and study the molecular characteristics, while when estimated the activity of lysis and clotting for peaks separate P1, P2 and P3 for wasp yellow toxin, the lysisactivity of separate P2 was 91% of the poison origin activity and the clotting time was 43.8 minutes, while the lysis activity of separate P1 was 49% of the poison origin activity and the clotting time was 19.28 minutes, and the lysis activity of separate P3 was 29% of the poison origin activity and the clotting time was 7.52 minutes, so P2 separate from wasp red poison was selected to purification and study the molecular characteristics The molecular weight of wasp red and yellow toxin the protein cm wasp red toxin P1 was 22387 Da and wasp yellow toxin P2 was 22382 Da using the gel filtration Sephacryl S - 200, while the carbohydrates content were 60.057 and 44.460 mg/ ml for wasp red P1 and yellow P2 toxin respectively as determined by phenol - sulfuric acid method, and type of link between carbohydrates and protein in the separate P1 and P2 investigating by using gel filtration Sephacryl S - 200 and absorbance at 280 and 490 nm.The percentage of iron in wasp red P1 and yellow P2 toxin were 0.88 and 0.44 ppm respectively as determined by atomic absorption, the contain proteins separate P1 and P2 on the iron were investigating by using gel filtration Sephacryl S - 200 and absorbance at 280 and 557 nm. The optimum pH of lysis and clotting activity for wasp red toxin P1 was 6.5, while lysis activity was decrease in pH 4 and 8 to be 38 and 79% respectively from its original activity, and clotting time was decreased from 47.3 min at optimum pH to be 17.9 and 37.3 min at pH 4 and 8, respectively, it was noted that wasp red toxin P1 was constant in the pH range of lysis activity from 6 - 7.5, but it lost 67 and 25% from its original activity at pH 4 and 8 respectively, while the clotting time was constant in the pH range from 6 - 7.5, but it decreased in pH 4 and 8 to be 15.6 and 35.4 minutes respectively The optimum pH of lysis and clotting activity for wasp yellow toxin P2 was 6.5, while lysis activity was decrease in pH 4 and 8 to be 18 and 37% respectively from its original activity, and clotting time was decreased from 48.8 min at optimum pH to be 8.7 and 35.6 min at pH 4 and 8 respectively, it was noted that wasp red toxin P2 was constant in the pH range of lysis activity from 5.5 - 8, but it lost 74 and 19% from its original activity at pH 4 and 8 respectively, while the clotting time was constant in the pH range from 6 - 7.5, but it decreased in pH 4 and 8 to be 12.6 and 39.5 minutes respectively The results for optimum stability temperature of lysis and clotting activity for wasp red toxin P1 refer to increase activity by increasing the action temperature until it reached a maximum at a temperature 35oC, then lysis activity was decreased to be about 4% of its original activity at 80oC, while the clotting time was decrease from 48.8 minutes at the optimum temperature to be 1.8 minutes at 80oC, it was noted that the lysis and clotting activity were stabile at 35oC for 30 min, but it is beginning to decrease with increasing temperature to loses 84% of its original lysis activity at 80oC, and the clotting time was decreased from 47.3 min at 35oCto be 2.3 min at 80oC.The results for optimum stability temperature of lysis and clotting activity for wasp yellow toxin P2 refer to increase activity by increasing the reaction temperature until it reached a maximum at a temperature 35oC, then Lysis activity was decreased to be about 8% of its original activity at 80oC, while the clotting time was decrease from 48.8 minutes at the optimum temperature to be 3.9 minutes at 80oC, it was noted that the lysis and clotting activity were stabile at 35oC for 30 min, but it is beginning todecrease with increasing temperature to loses 98% of its original Lysis activity at 80oC, and the clotting time was decreased from 48.8 min at 35oC to be 5.3 min at 80oC.The results refer to found a relationship between the influence of the wasp type and dose on the red blood cells lysis degree in males and females, it was observed that the concentration had a significant effect (p<0.05) in the red blood cells lysis degree in females, it was 0.401 at concentration 100% for wasp red toxin P1, while it was 0.331 at the same concentrate for wasp yellow toxin P2, the concentration also had a significant effect (p<0.05) in the red blood cells lysis degree in males, it was 1.020 at concentration 100% for wasp red toxin P1, while it 0.842 at the same concentrate for wasp yellow toxin P2, also it refer to found a significant effect (p<0.05) between the effect of the wasp red P1 and yellow P2 toxin and dose on the red blood cells clotting time in females, itwas 46.80 min at concentration 100% for wasp red toxin P1, while it was 46.20 min at the same concentrate for wasp yellow toxin P2, the concentration also had a significant effect (p<0.05) in the red blood cells clotting time in males, it was 47.80 min at concentration 100% for wasp red toxin P1, while it 40.47 min at the same concentrate for wasp yellow toxin P2, there was no significant effects for blood clotting time when making a comparison between the wasp red P1 and yellow P2 toxin for eachconcentration in females and males. The compared between red blood cells clotting time due the wasp red P1 and yellow P2 toxin dose in females and males did not refer to found a significant effect (p<0.05), the clotting time for wasp red P1 was 46.80 and 47.80 min at concentration 100% for females and males respectively, while the clotting time at concentration 6.25% was 17.30 and 17.10 min for females and males respectively, whilst the clotting time red blood cells due wasp yellow P2 toxin in females was 46.20 min and 47.40 min in males at concentration 100%, and it was 17.00 min in females and 17.10 min in males at concentration 6.25%.

دراسة مقارنة للتاثير الحامي للكبد بين الكويرسيتين ومستخلص الحلبة في ذكور الجرذان المسممة برباعي كلوريد الكاربون Comparative Study of The Hepatoprotective Effect Between Quercetin And Trigonella Fenum Graecum Extract In Carbon Tetrachloride Induced Hepatotoxicity In Male Rats

اسم المؤلف: نور حسون كاظم القرةغولي

اسم المشرف: سحر محمود جواد الجمالي محمد داخل الركابي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: يعد الكبد العضو الرئيسي المسؤول عن ازالة السموم في الجسم, مما يجعله الاول في التعرض للسمية الدوائية والكيميائية للمواد الداخلة للجسم من جميع الاعضاء, ووفق ذلك اجريت هذه الدراسة لمقارنة التاثير الحامي للكويرسيتين ومستخلص الحلبة على الانسجة الكبدية لذكور ال | The liver is the main organ responsible for removing toxins in the body, making it the first to face various chemicals from all of the organs, and according to this the study had designed to compare the hepatoprotective effect of the Quercetin and Trigonella foenum graecum extract on the liver tissues of male rats poisoned with carbon tetrachloride, and to know their effects when used together. The study was conducted at the Faculty of Pharmacy - University of Kufa, and included using of 30 rats from Sprague - Dawley divided into five groups : the negative control group, the positive control group, the group treated with organic extract, the quercetin treated group and the group treated with extract and quercetin together. The period of dosing continued for 7 days and on the eighth day all groups(except the control group) were poisoned with carbon tetrachloride. After 24 hours the animals were killed. The study lasted from April 2013 - until July 2013. The Liver weights of treated animals were measured, the effectiveness of transferase enzymes Alanine Transaminase, Aspartate Transaminase and Alkaline phosphatase and total bilirubin level in the serum were assessed, also the Glutathione and Malondialdehyde levels were estimated in liver homogenized. The results showed the following : A significant increase (P<0.05) in the liver weights, the effectiveness of the amine transferase enzymes, Alkaline phosphatas and total bilirubin in the serum of the treated animals when compared with the negative control group, while these parameters revealed a significant decrease (P<0.05) at the treated groups as compared with the positive control group. The results showed no significant difference (P>0.05) in the Glutathione level and a significant increment (P<0.05) in the level of Malondialdehyde in the treated groups when compared with the negative control group. Also, the results showed a significant decrease and increase (P<0.05) in the levels of Glutathione and the Malondialdehyde at the treated animals as compared with the positive control group. The present study showed numerous histological changes which resulted from the injection with carbon tetrachloride. In relation with the tissue sections which have been taken from the treated groups : with the extract, quercetin and the group treated with both revealed a protective effect for organic extract seeds of Trigonella foenum - graecum and quercetin drug on the liver tissues which had been poisoned and the best effect in the protection appeared at the group which was treated with the extract and quercetin together. From this study we can conclude that the Trigonella foenum - graecum organic extract and quercetin may reveal a protective role for the liver against carbon tetrachloride poisoning by maintaining the effectiveness of liver enzymes Alanine Transaminase, Aspartate Transaminase and Alkaline phosphatase and bilirubin level in the serum as well as by increasing the Glutathione level and falling the Malondialdehyde level in liver homogenized. Also we conclude that using Trigonella foenum - graecum organic extract and quercetin together led to double protection of the liver and efficiently as a result of the positive impact which resulted from using them together

تقييم كفاءة بعض المواد الكيميائية ومستخلص الماء المغلي والبارد لنبات الصبار (L.) Opuntia ficus indicaعلى حيوية الرؤيسات الاولية للمشوكة الحبيبية Echinococcus granulosus خارج الجسم الحي In Evaluation The Efficiency of Some Chemicals And Boiled And Cooled Water Extracts of Cactus Opuntia Ficus Indica (L.) On The Protoscolices Viability of Echinococcus Granulosus In Vitro

اسم المؤلف: اشجان محمد عريبي الخالدي

اسم المشرف: احمد خضير عبيس الحميري

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

اسم المؤلف: مقداد احمد شهاب

اسم المشرف: هاشم محمد عبد الكريم العلاق

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية العلوم - قسم علوم الحياة

اللغة: العربية

النص الكامل من المصدر: https://qu.edu.iq/repository/wp-content/uploads/2017/04/%D8%B1%D8%B3%D8%A7%D9%84%D8%A9-%D9%85%D9%82%D8%AF%D8%A7%D8%AF-%D8%A7%D8%AD%D9%85%D8%AF-%D8%B4%D9%87%D8%A7%D8%A8-ilovepdf-compressed.pdf

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: The present study had been carried out to elucidate the toxic effects of sodium fluoride on local rabbit males, this study involved determination of the histological Changes of some organs include (Testes, Liver and Kidney) , moreover study the changes in body weight of the animals and the percentage of organs weight and some blood parameters and biochemistry which include packed cell blood volume (PCV), Hemoglobin (Hb), Total white blood cells, Testosterone hormone, T3, T4 hormones, Glucose concentration, Cholesterol, Urea, Transaminase enzymes and Bilirubin - level in blood. Twenty local adult male rabbits were included this experiment their ages ranged between 10 - 14 months.The animals were treated with oral swallow for 12 weeks. The animals were divided into four groups : control group is treated orally with (0.9%) normal saline; First group was treated orally with 10 mg/kg of body weight sodium fluoride, second group was treated orally with 20 mg/kg of body weight sodium fluoride and third group was treated orally with 30 mg/kg of body weight sodium fluoride. The results of present study showed a significant decreased (P<0.05) in body weight of treated animals with dose of sodium fluoride 20 and 30 mg/kg of body weight, while there is no significant differences in the body weights of treated animals with sodium fluoride dosage of 10 mg/kg of body weight when to compared with control group. The percentage of organ weight to body weight showed a significant decrease in testis weights and liver weight, while there was no significant differences in kidney weight in treated group with sodium fluoride when to compared with control group. Concerning histological changes showed a reduction or freezed stages in spermatogenesis processes which timed with decrease in number of primary spermatocytes and secondary spermatocytes in addition to infiltration of lymphocytes, also found that NaF cause disorganization, denudation, and reduction in germinal epithelial cells of the seminiferous tubules and associated with absence of sperm in the lumina, also showed a decrease in radius of epididymus ducts and decrease in number of sterocilia and decrease number of mature sperms. Histological sections of kidney showed that there was a clear shrinkage of glumeruli.Histological section of kidney of animals that treated with sodium fluoride showed a mild blood vessels congestion, shrinkage in glumeruli, distributed in renal tubules and erode its epithelium with some apoptotic areas. The liver sections appeared appreciable effect with fluoride include dilation in central vein and erode to its epithelium, congestion in hepatic sinusoids with apoptosis and erode to general structure of liver with some of inflammatory cells and death in some cells which lead to erode of typical ray arrangement. Regarding blood parameters the present study showed a significant decrease (P<0.01) in percentage of (PCV) and (Hb) of the animals treated with sodium fluoride, while showed (WBCs) count significant increased compared with control group. Hormonal analysis (Testosterone, T3 and T4) showed a significant decrease, while significant increase of glucose, cholesterol, urea, transaminases enzymes for (AST, ALT) and bilirubin levels. Results obtained from this study and mentioned above may be attributed essentially to that of Naf administration results in sever lesion of male reproductive system wich may be aggravated with increase administration concentration of Naf.

انتشار داء اللشمانية الجلدية في محافظة القادسية مع تقييم مدى الاسجابة للعلاج بعقار البنتوستام بطريقة RT - PCR Prevalence of Cutaneous Leishmaniasis In Al - Qadissia Province And The Evaluation of Treatment Response By Pentostam With Rt - Pcr

اسم المؤلف: رنا صالح صاحب محلول الدفاعي

اسم المشرف: غيداء عباس جاسم الجبوري

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: Results of clinical and laboratory examination of 239 cases showed 55 infection cases in Cutaneous Leishmaniasis and in infection proportion reaches at 23.01% out of the total number of the Cutaneous cases which are similar to Leishmaniasis during the months of the study. The Results also showed that the highest infection was recorded between the ages 1 - 10 years old and about 26 cases in 47.27% and between 10 - 20 years old about 17 cases in 30.9% and the lowerest was between 40 - 50 and 70 - 80 years old in one cases in 1.8%. The summit of the disease spread was in February for about 24 cases in 43.63% specially in AL - Hamza AL - Sharqi where the highest number of infection was recorded for about 23 cases in 41.81%. It is followed by AL - Shafiya for 10 cases in 18.18% and the lowerest was AL - Mhannawiya for one case in 1.8%. It has been clear that the number of the multiple epidemics is more than the individual ones in 70.5% while the latter in 29.98% respectively. It was focused on hands and legs in 50.35% and 24.46 %. The tissue test showed thick perspiration in the big infected pharynx and lymphatic cell in addition to the existence of abscess to the smash of the infected cell. Forty nine positive cases were found in 89.09% for the genetic material DNA which is connected with Leishmaniasis by mean of NestedPCR to identify the type results showed 47 samples which is L. major in 95.91% and to get sure by investigating the virulence factor lipophosphoglycan, proteophosphoglycan and cysteine protease it was found that all sample were positive in 100% as Leishmania. major. To know the affect of treatment pentostam on these samples, the standard proportion for the genetic patterns was calculated factor lipophosphoglycan, proteophosphoglycan and cysteine protease during five week. Results showed that there was a big difference for each gene in a solation during the period of treatment in a level of the possibility of (P?0.05) when used test LSD. and by comparing the three genes it was found that there was a meaningful between cysteine protease and the other two factor lipophosphoglycan, proteophosphoglycan and there wasn’t difference between lipophosphoglycan, proteophosphoglycan. Hence, it has been clear that the treatment pentostam has a big affect in parasite by its affect on the virulence factors and this explains that possibility of infection may happen again after having the treatment. The skin disease cutaneous Leishmaniasis is considered as one of the endemic parasitic diseases in Iraq. This study aims at identifying its prevalence and specifying the virulence factors in Cutaneous Leishmaniasis in order to define the type of disease reason and the affect of the treatment of Pentostam on the virulence factors lipophosphoglycan, Cysteine protease, proteophosphoglycans by means Real time - PCR

دراسة بيئية للدايتومات الملتصقة على بعض النباتات المائية في هور العودة ضمن محافظة ميسان جنوب العراق A Study On The Ecology of Epiphytic Diatom On Some Aquatic Plants In Al - Auda Marshes / Maysan Province / Southern Iraq

اسم المؤلف: الاء عيسى موسى البوعجي

اسم المشرف: جنان شاوي الحساني

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: البيئة

الدرجة: ماجستير

الجامعة: جامعة بغداد - كلية العلوم للبنات

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: اجريت الدراسة الفصلية على الدايتومات الملتصقة على النباتات المائية لثلاث مواقع في هور العودة ضمن محافظة ميسان جنوب العراق (من شهر تشرين الاول 2013 لغاية شهر تموز 2014) نظرا لاهمية الدايتومات في الانظمة البيئية المائية والاهمية البيئية لاهوار العراق على مس | Seasonal study on the quality and quantity of epiphytic Diatoms was conducted in the Al - Auda marsh within Maysan Province throughout one year from October 2013 to July 2014.These Diatoms have an important role in biotic ecosystems. Three taxa of aquatic plants were selected (Phragmites australis Trin ex stand, Ceratophyllum demersum L. and Typha domengensis Pers) in three sites within Al - Auda Marsh; these sites were Al - Adleh, Al - Auda and Um - Almashahef. Also, the study included measuring physical and chemical factors of all the study sites, such as : temperature (air and water), power of hydrogen (pH), total hardness(TH), Calcium (Ca), Magnesium (Mg), turbidity (Tur.), light penetration(LP), depth of water (D), total dissolved suspend material (TDS), total suspended solids (TSS), total alkalinity (TA), dissolved oxygen (DO), total nitrogen (TN) and total phosphorus (TP) and reactive Silicate (SiO3). The mean ranges of the studied factors were recorded : air temperature (10 - 38 C°), water temperature (13 - 34 C°), pH (7.1 - 8.6), TH (672 - 3800mg/l), Ca (97.8 - 264.5 mg/l), Mg (125.1 - 896.13mg/l), Tur.(7.62 - 30.7NTU), LP(46 - 113cm), D (190 - 413cm), TDS (1170 - 3075 mg/l), TSS (0.02 - 0.08 mg/l). while TA (207.5 - 300mg/l), DO (6.4 - 13.5 mg/l), TN (2.41 - 8.58µg/l), TP (0.013 - 0.23 µg/l) and SiO3(101 - 776.77 µg/l).Results of the study indicated presence of 111 taxa of epiphytic diatoms, which belonged to 13 families and 26 genus (one family and two genus of centric diatoms, 12 families and 26 genus of pennate diatoms).The present study recorded 21 species that were found only on one host plant, 20 species that were found on two host plants and 70 species that were found on all host plants, nine of diatoms taxa were present in all host plants among all study seasons which are Achnanthes minutissima, Cocconeis placentula var. euglypta, Cyclotella meneghiniana, Synedra affinis, Syneda ulna , Gomphonem parvulum, Mastogloia smithii var. amphicephala, E. zebra var. porcellus, Rhopalodia gibba.One of the diatoms taxa was recorded in the present study is new to Iraqi flora, the new record of diatom include one taxa of Achnanthidiaceae family (Achnanthes exigua var. constricta). The results of this study included description of the basic characteristics of this taxa with complete photographic documentation.It was evident, from the results, that the morphological form of host plants influenced the number of the attached epiphytic diatoms. C. demersum had attracted 92 diatoms taxa(35%) while the lowest number was 82 taxa (31%) attached by Typha domengensis. The seasonal variation of diatoms growth was evident. Spring 2014 was characterized by the highest number of attached taxa (69 on P. australis - 11%) whereas the lowest number was observed on C. demersum (32 taxa - 5.4%) in autumn 2013.Numbers of epiphytic diatoms fluctuated among study sites, seasons and aquatic plants. The total number of epiphytic diatoms on P. australis ranged between 0.73×104 cell/gm wet weight in summer 2014 at Al - Auda and 406.89104 cell/gm wet weight in spring 2014 at Um - Almashahef. C. demersum recorded highest total number 513.9 ×104 cell/gm wet weight in winter2014 at Al - Adleh, while the lowest total number was 0.1×104 cell/gm of wet weight was recorded in the spring 2014 at Um - Almashahef. While total number of taxa on T. domengensis ranged (0.84 - 427.89) ×104 cell/gm wet weight in summer and winter 2014 at Um - Almashahef.Fluctuations in the total number of main epiphytic diatoms families were observed on all aquatic host plants throughout the study period. The higher total number(92.59×104 cell/gm of wet weight) of diatoms recorded in Achnanthidiaceae family in summer 2014 on P. australis and lower total number (0.28×104 cell/gm of wet weight) on same plant observed in Rhoicospheniaceae family in spring 2014. C. demersum showed highest total number (95.15 ×104 cell/gm of wet weight) in Fragilariaceae family in spring2014, while the lower total number (0.3×104 cell/gm of wet weight) was in family Rhoicospheniaceae in autumn 2013. Individual of Achnanthidiaceae family were recorded as highest total number (168.57×104 cell/gm of wet weight) on T. domengensis in Achnanthidiaceae family in the winter of 2014, but lower total number (0.28×104 cell/gm of wet weight) recorded in Coscinodiscaceae family in the same season. The study showed highest values for richness index (3.77) in the spring of 2014 for epiphytic diatom on C. demersum in Al - Auda site, while lower richness index recorded was (0.6) on T. domengensis in theautumn of 2013 at Um - Almashahef. Shannon - Weaver index ranged (0.24 - 2.45) for epiphytic diatom on P. australis during winter of 2014 at Um - Almashahef and autamn 2013 at Al - Auda, respectively. Highest values for Evenness index were recorded (0.28) for epiphytic diatom on C. demersum in the spring of 2014 at Um - Almashahef site, while for the same site lower values recorded was (0.03) on T. domengensis in winter 2014. Higher percentage for Jaccard similarity index (63.2%) obtained between epiphytic diatoms hosted on T. domengensis and lower percentage of similarity (28.9%) found among the common taxa of epiphytic diatoms on C. demersum, and highest percentage for Jaccard similarity index between host plants was (70%) between P. australis and C. demersum while lower percentage of similarity(67%) was obtained between T. domengensis and C. demersum.

دراسة عدد من المتغيرات الفسلجية والمناعية لدى النساء المصابات بالتهاب السبيل البولي في مدينة تكريت Study Some of Physiological And Immunological Parameters in Women With Urinary Tract Infections in Tikrit City

اسم المؤلف: لبنى عبد الجبار ياسين

اسم المشرف: موسى جاسم محمد الحميش

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: ان الهدف الرئيس لهذه الدراسة هو معرفة علاقة الانترلوكينات(IL - 2, IL - 4, IL - 6) ببعض الهرمونات لدى النساء المصابات بالتهاب السبيل البولي.تضمنت هذه الدراسة 116عينة دم من النساء المراجعات للعيادات الخارجية التابعة لمستشفى تكريت التعليمي اللواتي تترواحت اع | The main objective of this study is to investigate the relationship of Interleukines (IL - 2, IL - 4, and IL - 6) with hormones in women with inflammation of the urinary tract. This study involved (116) women visiting outpatient clinic of Tikrit Teaching Hospital for the period from September 2011 until February 2012. The ages of women were 16 - 50 years, and blood samples were taken for each of these women for the purpose of determining the levels of sex hormones (FSH, LH, estrogen, progesterone, testosterone) and the levels Interleukines (IL - 6, IL - 4, IL - 2) and identify some of the variables blood parameters (WBC, ESR) and C - reactive protein Twenty samples were taken from the blood of women who are not infected and non - pregnant as a control group samples. The patients divided into three groups, which included non - pregnant women infected and pregnant women who are infected and pregnant non - infected urinary tract (UTI) also samples of the study was divided by periods of gestation into three periods included the first period (1 - 12 weeks) and the second period (12 - 24 weeks) and the third period (24 - 40 weeks) of pregnancy The results of the current study showed that there is significant increase in ESR for a pregnant women infected and pregnant women non - effected with UTI when compared to control (P ? 0.01) and highest level of ESR was in the last third of pregnancy, The total Count of white blood cells (WBC), results showed a significant difference between the three groups compared to the control group, had the highest level for (WBC) in the last third of pregnancy. As for the study of hormones when measuring the level of the hormone LH showed a significant decrease (P ? 0.05) for the three groups compared to control and the steepest decline of hormone level was in the last third of pregnancy. The hormone FSH got a significant decrease in the three groups compared with a control (P ? 0.01), lowest level was in the third trimester of pregnancy.Estrogen level showed a significant decrease (P ? 0.01) for a non pregnant women with UTI compared with a control, and show a significant increase in the level of the two sets of hormone - positive pregnant non - infected, and the highest increase was in last third of Pregnancy in both groups. The progesterone showed a significant increase in the level of the pregnant infected and pregnant non - infected (P ? 0.01) compared with a control group. While in non - pregnant, there is no significant difference. The testosterone level increased significantly (P ? 0.05) in the three groups compared to control group. the level Interleukines, Tests showed that a significant increase(p ? 0.01) in the level of interleukin - 2 (IL - 2) for a pregnant women with (UTI) compared with a control and a significant decrease in the last third of pregnancy in pregnant women which is non infected compared to the rest of the gestation periods, and for the level of interleukin - 4 The present study showed a significant increase (p ? 0.01) in the level of interleukin - 4 (IL - 4) for a non - pregnant women with (UTI) compared with a controlThe highest level reached by IL - 4 was in the last third of pregnancy in the non - infected at (p ? 0.05), interleukin - 6 has a significant decrease for the two sets of pregnant women with (UTI) and non - infected, compared with the control group and the absence of significant differences between the two sets of pregnant women infected and non - infectedRegarding level of C - reactive protein there is a significant increase (P ? 0.01) in the two groups of pregnant and non pregnant women compared with a control, and significant differences between control group and a group of pregnant non - infected.

دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتية على نمو الطفيلي في الزجاج في مدينة كركوك Epidemiological Study on Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction on The Parasite in Vitro in Kirkuk City

اسم المؤلف: محسن عز الدين سليمان

اسم المشرف: ابراهيم شعبان داوود محمد عبد العزيز قادر

الموضوع العام: علوم الحياة

السنة: 2008

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

تقييم استخدام الانترليوكينات في تشخيص تسمم الدم الجرثومي لدى الاطفال حديثي الولادة في تكريت Evaluation Uses The Interleukines for Diagnosis of Neonatal Sepsis in Tikrit

اسم المؤلف: هبة حازم صالح

اسم المشرف: موسى جاسم محمد الحميش عاشور رفعت سرحت

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: ان الهدف الرئيس لهذه الدراسة هواستخدام الانترلوكيناتInterleukin - 8, Interleukin - 10 (IL - 8, IL - 10) كعلامة تشخيصية للتسمم الدموي الجرثومي لدى حديثي الولادة. تضمنت الدراسة 45 عينة دم من الاطفال حديثي الولادة الراقدين في ردهة الاطفال في مستشفى تكريت الت | The main aim of this study was uses the Interleukines IL - 8 and IL - 10 as diagnosis marker for neonatal sepsis. This study involved 45 blood samples, were taken from neonates admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically diagnosed as sepsis for the period from September 2011 until March 2012. The ages of neonates were 1 - 30day, and this blood samples were taken for the purpose of determining the levels Interleukines IL - 8 and IL - 10 and identify (WBC and C - reactive protein). Seventeen samples were taken from the blood of neonates without of septicemia as a control group samples. The results of the current study showed non significant differences in total white blood cells count for patients with sepsis when compared with a control group. and showed non significant differences in total white blood cells count for patients with sepsis as gestation birth, sex and birth weight compared to the control group. Regarding level of C - reactive protein there was no significant differences between the control group and the patients with sepsis as gestation birth and sex. and showed a significant differences in C - reactive protein level for patients compared to the control group as birth weight. The Interleukine levels showed that a significant increase in the level of interleukin - 8 (IL - 8) for all patients mals and femals, preterm or full - term and who there weight < 2.5 or >2.5kg compared with a control group. this results showed that the relationship between IL - 8 and WBCs was not significant differences at (p<0.05). and for the level of interleukin - 10 the present study showed a significant increase for patients with sepsis as gestation birth, sex and birth weight compared to the control group, the relationship between IL - 10 and WBCs was not significant differences at(p<0.05). The relationship between IL - 1o, IL - 8 and WBCs was not significant differences. when comparison The results as end result for sepsis show is the absence of significant differences (p<0.05) for patients who became good and who had complications compared with the control group. At last, the study concluded that the Interleukines IL - 8 and IL - 10 levels increase in early time for Inflammation ;So its agood diagnosis marker for neonatal sepsis.

تقيم بعض العناصر النادره ومستوى المالونداي الدهايد والبروتين في الرجال العقيمين Assessment of Some Trace Elements, (MDA) And Protein Levels In Infertile Men

اسم المؤلف: زهراء فلاح عبد العالي عنوز

اسم المشرف: علاء الدين صبحي محسن السلامي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية العلوم - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: تم اجراء هذا البحث لدراسه عينات السائل المنوي التي تم الحصول عليها من المرضى المصابين بوهن النطف وعددهم 35عينه كذلك سوي النطف عددهم 40 عينه وتم اخذ مجموعه من الاشخاص الاسوياء (مجموعه السيطره) حيث كان عددهم 20 عينه الذين راجعو مركز الخصوبه في مدينه الصدر ا | This study was performed on human semen specimens obtained from Asthenozoospermic patients (35 specimens) Normozoospermic males (40 specimens), and (20 specimens) Fertile Control group, who were attending to the laboratories of Fertility center in ALSader Hospital of AL - Najaf AL - Ashraf city during the period extended from 1 - 9 - 2013 to 30 - 1 - 2014. The aim of This present study was to estimate the levels of some Trace element (Lead, Copper, Cobalt, Chromium, , and Cademium) concentrations in Asthenozoospermia and Normozoospermia. by atomic absorption method, and comparsion with control (Fertile).Moreover, it tends to estimate the concentration of (MDA) Malondialdehyde , Total protein concentration and to know the viability sperm percent in semen specimens.Also, the relationships between mentioned components and some semen parameters. The results revealed significant increase (P <0.05) in the concentration of examined trace elements in Asthenozoospermia and Normozoospermia when comparsion with control, also a significant increase (P <0.05) in total protein concentration, also in MDA. While The results revealed significant decrease (P <0.05) in the viability sperm percent in semen specimens. Correlation study showed positive relationship between concentration of the trace elements and abnormal sperm morphology percent, also with total protein concentration, and MDA concentration. While negative relationship between concentration of trace elements and percent of sperm motility.It was concluded that the increase in the concentration of the trace elements and it influence on blance and parameter of seminal fluid, also the Correlation between trace element and Protein in semen. All of this lead to arise of cases of Asthenozoospermia and Normozoospermia infertile patients.

عزل وتشخيص بعض انواع الجراثيم المسببة لالتهابات الزائدة الدودية في مدينة تكريت Isolation And Identification of Some Baceterial Speices Which Caused Appendices Infections in Tikrit City

اسم المؤلف: عبد عبد الله محمد الجبوري

اسم المشرف: اميرة محمود محمد الراوي علي صالح حسين الجبوري

الموضوع العام: علوم الحياة

السنة: 2010

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

اسم المؤلف: ايناس صادق عبد الكريم

اسم المشرف: عماد احمد محمود حسن سعيد الاسدي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان - الحشرات

الدرجة: ماجستير

الجامعة: جامعة بغداد - كلية العلوم للبنات

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

اسم المؤلف: توركان احمد حمد حسن

اسم المشرف: برهان مصطفى محمد الدليمي عواد شعبان داود الناصري

الموضوع العام: علوم الحياة

السنة: 2008

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

انتاج انزيم السيليلز من عزلة محلية لبكتريا B167 Streptomyces sp. واستخدامه في انتاج الوقود الحيوي Cellulase Production From Local Isolate of Streptomyces Sp.B167 And Its Application In Biofuel Production

اسم المؤلف: بنان محمود سليمان

اسم المشرف: ناظم حسن حيدر

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: التقنيات الاحيائية

الدرجة: ماجستير

الجامعة: جامعة بغداد - كلية العلوم - قسم التقانة الاحيائية

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: هدفت الدراسة الحالية الى انتاج انزيم السليليز من بكتريا الستربتومايسس ودراسة تاثير بعض الظروف الزرعية على انتاج الانزيم; انتاج الوقود الحيوي من المخلفات السليلوزية من خلال التحلل المائي للمخلفات بالاحماض والانزيمات المايكروبية. تم غربلة 74 عزلة لبكتريا St | The current study was aimed to produce biofuel from cellulosic waste material degraded by local isolate Streptomyces. Seventy four isolates of Streptomyces were screened for cellulase production in solid and liquid media. The results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose. Therefore, it was selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the isolate Streptomyces sp. B 167 reached to 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) by supplementation of cellulose liquid medium with 1 % of yeast extract as nitrogen source. Additives of carbon sources like (manitol, glucose, maltose, sucrose and starch) to the process of saccharification did not improve the cellulase productivity. The bioconversion of cellulosic waste to reducing sugar was maximum with Banana peels (77.78 %) followed by the rice husk (75.56 %), orange peels (71.11 %), corn steep peels (60.0 %) and lowest bioconversions (53.33 %) were recorded with sawdust. The degradation of cellulosic waste increased with increasing substrate concentration. Maximum cellulase productivity (3.18 U/ml) and bioconversion (86.1 %) was obtained at 3 % (w/v) of cellulosic waste (Banana peels). Saccharification of cellulosic waste with different treatment methods was studied. The pretreatment of cellulosic waste with 1 % HCl and H2SO4 produces 21 and 15.8 g of reducing sugar / 100 g of cellulosic waste. In comparison, hydrolysis with Streptomyces sp. B 167 enzymes resulted a significantly higher amount of reducing sugar yield (25 g / 100 g cellulosic waste). Further fermentation of cellulosic hydrolysates was performed using Saccharomyces cerevisiae using stationary fermentation condition. Maximum yield of ethanol were (0.30, 0.19 and 0.10 g ethanol / g glucose) observed with Streptomyces sp. B 167 enzymes, HCl and H2SO4 hydrolysates respectively after 48 h of fermentation

تاثير المستخلص الكحولي لاوراق نبات ذيل العقرب Heliotropium europium وفيتامينC في بعض الجوانب الفسلجية والكيموحيوية لذكور الجرذان المعرضة للكرب التاكسدي Effect of Heliotropium Europium Leaves Alcoholic Extract And Vitamin C in Some Physiological And Biochemical Parameters of Male Rats Exposed To Oxidative Stress

اسم المؤلف: صفا احمد عبد القادر محمد دنيدل

اسم المشرف: صاحب جمعة عبد الرحمن عويد هيدان الخزرجي

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: هدفت الدراسة الحالية الى معرفة تاثير المستخلص الكحولي لنبات ذيل العقرب Heliotropium europium في وزن الجسم وعدد من المتغيرات الفسلجية والكيموحيوية كتركيز الهيموكلوبينHaemoglobin (Hb) وحجم كريات الدم المرصوصةPacked cell volume (PCV) والعدد الكلي لخلايا الدم | The aim of the present study was to investigate some effects of the alcoholic extract of Heliotropium europium in body weight and some of physiological and biochemical parameter such as concentrations of Hemoglobin (Hb), Packed Cell Volume (PCV) and the total count of leukocytes (WBCs) and concentrations glutathione (GSH), Malondialdehyde (MDA), Peroxynitrite radical (ONOO - ) , Superoxide dismutase enzyme (SOD), glucose, Total Cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Very Low density lipoprotein (VLDL - C), Atherogenic indices levels, Total protein, Albumin, Globulin, Urea and Uric acid in serum and histological changes in liver of male rats that exposed to oxidative stress induced by hydrogen peroxide H2O2(0.5%) with drinking water for (30) days, and comparison these effects with the effects of the vitamin C as well known antioxidants(250 mg/kg of body weight), for the purpose of this study preliminary experiments were carried out to find the most effective does of plant extracts and it was found that this does was (300 mg/ kg) body weight.The study included(35) male albino rats, (4 - 6) months ages and(245 - 280)gm weight were divided to (7) groups both group with (5) rats as follows : Control group, hydrogen peroxide (H2O2) group, Heliotropium extract group, H2O2+ Heliotropium extract group, Heliotropium extract + vitamin C group, H2O2+ Vitamin C group, H2O2+extract of Heliotropium+ vitamin C group.The results showed : 1. The animal under oxidative stress by hydrogen peroxide caused a significant decrease (p? 0.05) in body weights and values of Hb , PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin, globulin and compared with the control group. While it caused a significant increase in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels(I, II, III) and urea compared with the control group.2. The treatment of animals exposed to oxidative stress induced by hydrogen peroxide with extract of Heliotropium europium showed significant increase in the weights of animals, Hb, PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin and globulin compared with a hydrogen peroxide group. While this treatment showed significant decrease in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels (I, II, III), and urea when compared with a hydrogen peroxide group.3. Compared with a hydrogen peroxide treatment of rats exposed to oxidative stress by hydrogen peroxide with vitamin C, extract of plant and vitamin C with hydrogen peroxide group showed the results were similar to the results of treatment plant extract with hydrogen peroxide group.4. The treatment plant alcoholic extract and vitamin C showed a significant increase in the level GSH, MDA, activity of SOD enzyme, HDL - C, globulin compared with the control group. And showed a significant decrease in animal weights, WBCs, ONOO. - , LDL - C, VLDL - C and urea compared with a hydrogen peroxide group. While not shown significant differences in the values of Hb, PCV, glucose, cholesterol, TG, Atherogenic indices levels total protein and uric acid compared with the control group.5. The treatment alcoholic plant extract group showed increase in the weights of animals and values Hb, PCV, WBCs, GSH, MDA, Triglyceride, VLDL - C, Atherogenic indices levels, globulin and urea compared with the control group. Showed significant decrease in the HDL - C and albumin compared to the control group. while did not show significant difference in ONOO. - , activity SOD enzyme, glucose, cholesterol, total protein and uric acid compared the control group.6. The most important histological changes that have occurred in this study have showed treatment hydrogen peroxide compared with a control to swelling hepatocytes with necrosis each and the small size of the nuclei with swelling sinusoid, and the treatment of animals exposed to oxidative stress plant extract has showed a lack of clarity within the cells in hepatocytes necrosis and swelling sinusoid compared with a hydrogen peroxide while the treatment of animals exposed to oxidative stress with vitamin C showed sinusoid constriction and showed treatment alcoholic plant extract and vitamin C for the animals exposed to oxidative stress loss nucleus cells with the difficulty of distinguishing sinusoid.

تاثير مستخلص الثوم والرمان على الجرذان المصابة تجريبيا بطفيلي Cryptosporidium parvum ومقارنتها مع عقار Metranidazole Effect Extract Allium Sativum And Punica Grantium On White Rat Expermental Infection Cryptosporidium Parvum And Comper With Metranidazole

اسم المؤلف: سرى رزاق خضير عبادة

اسم المشرف: نجم عبد الواحد عبد الخضر الجدوع

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية العلوم - قسم علوم الحياة

اللغة: العربية

النص الكامل من المصدر: https://qu.edu.iq/repository/wp-content/uploads/2017/04/%D8%B1%D8%B3%D8%A7%D9%84%D8%A9-%D8%B3%D8%B1%D9%89-ilovepdf-compressed.pdf

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: جمعت عينات البراز من الاطفال في مستشفى النسائية والاطفال كذلك جمعت من العجول المصابة حيث اجري فحص العينات بصبغة الزيل نلسن ثم عزلت ونقيت الاكياس بطريقة التطويف بمحلول شيذر السكري وجرعت 2103xكيس بيض لكل جرذ بانبوبة التجريع الفموي وسجلت المدة قبل البائنة من | Collects stool samples from children in the women's and pediatric hospital. also collects all those from infected calves, where he conducts testing samples dye Zail Nelson then isolated and scrubbed bags manner floation solution Shidhir diabetes and gived rat 2 x103 bag eggs per rat tube dosage oral and recorded for the period before dowry of 3 - 5 days as recorded wounding 100%. The result is the appointment of the lethal dose of moderation LD50 of aqueous extracts of the garlic, peel the pomegranate after giving the rats doses graded by the mouth and the results indicate that the dose 7500 mg / kg of body weight is the lethal dose of aqueous extracts of garlic bulbs and dose limits of 5250 mg / kg is the lethal dose to peel pomegranate then tretment using cold aqueous extract to plant garlic. Allium satvuim L. peel and pomegranate Punica granantum L and drug Metronidazole (Flagyl) concentration of 250, 500, 750 mg / kg, the results shows the effectiveness of the current study, the aqueous extracts in the treatment of the parasites it turns out that the time required to kill the parasite suit inversely with the increasing concentration of extracts also show the efficiency of the aqueous extract of garlic bulbs and metronidazole concentration of 750 mg / kg in the treatment of 100% after 13 days of treatment with stops put bags of egg either concentration of 500 mg / kg of aqueous extracts garlic bulbs and metronidazole has shown the efficiency of treatment of by 100 % after 15 days of treatment showed, while the aqueous extract of pomegranate peel concentration 750 mg/kg therapeutic efficiency of 98.41% after 15 days of treatment, while the control group continued to launch until after the egg sacks (15) of treatment. The study shows histopathological section on the small intestine (duodenam, ileum, jejunum) Rats infection parasite get pathological changes represented by the crash of the villi of the small intestine and change epithelium from the vertical to the cube and the adhesion of some of the villi and gatherings of inflammatory cells in layer plate fitted, as well as for necrosis and desquamation of the cells lining the villi also destroyed gland and appeared egg sacks in the intestine result use 2x103 frome egg sacks also appeared effective abstract aqueous garlic in the intestinal tissue to restore its natural form after 15 day use concentration 750 mg/kg of aqueous extract of garlic bulbs and metronidazole while have taken normal sat to tissue treaement with aqueous pomegranate peel long than 15 day. test the effectiveness of aqueous extracts cold of garlic bulbs. Alluium sativum L. and peel pomegranate Punica granantum L in treatment parasite spores Cryptosporidum parvum and compared with the treatment of Metronidazole (Flagyl) in laboratory rats. Conducted on plant extracts cold of garlic and pomagranite the chemical tests have shown the presence of active substances of both alkaloids, carbohydrates, Flavonides, Alglycosidat, Alsaponyat, Alresins, phenolies

تاثير بعض العوامل البيئية والمايكروبايولوجية في تنقية مياه الشرب لمحطة المجمع السكني في قضاء الدور - محافظة صلاح الدين Effect of Some Ecological And Microbiological Factors on Drinking Water Purification Plants in Al - Mugama Al - Sakany in Al - Dour City - Salahaldin

اسم المؤلف: فخر الدين نور الدين رشيد الخفاف

اسم المشرف: حميد سلمان خميس المهداوي

الموضوع العام: علوم الحياة

السنة: 2009

الموضوع الدقيق: البيئة

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: تضمنت الدراسة جمع عينات شهرية من مياه نهر دجلة ومحطة مياه الشرب العائدة للمجمع السكني في الدور اخذت العينات من خمس محطات مختارة ابتداء من شهر تشرين الثاني 2007 ولغاية شهر تشرين الاول 2008 لغرض دراسة بعض العوامل الكيمياوية والفيزياوية والبايولوجية السائدة | This study was included collecting of monthly water samples from Tigris river and water purification plant in AL - Mugama AL - Sakany in AL - Dour, These samples were collected from five chosen locations, started from November 2007 until October 2008, To Study The dominance of some chemical, physical and Biological factors Through the year its effects on water purification plants in AL - Mugama AL - Sakany in AL - Dour. These factors included air and water temperature, Turbidity, Electrical conductivity, pH, dissolved Oxygen, BOD, Total alkalinity, Total hardness, Calcium and Magnesium hardness , Chloride reactive nitrite, nitrate phosphate and salinity as well as some Biological factors such as Total number of bacteria and diatoms during the study period. The results of this study shown that there was a seasonal variation in some chemical and physical factors. where the value of air temperature ranged between (3.5 - 40) C and water temperature ranged between (3.4 - 32.5) C.Turbidity value ranged from (2.2 - 22.94) NTU while electrical conductivity ranged between (320 - 590) µs / cm. The results also showed that the water in Tigris river was alkaline, where the pH value ranged between (7.08 - 7.98). and aeration. The avarices value of dissolved oxygen ranged between (3.5 - 10.2) mg/L and The BOD value never increase to critical value, its ranged between (1.58 - 2.37) mg /L salinity ranged between (0.110 - 0.305) mg/L while chloride concentration ranged between (21.78 - 23.82) mg / L. It has been found that total hardness ranged between (170 - 370) mg/L while total alkalinity for the five station ranged between (124.1 - 137.8) mg No2/ L while Micronutrients ranged from (0.388 - 0.449) mg / L and the value of nitrate ranged between (ND - 0. 598) mg No2/ L and (0. 65 - 122) mg Po4 / L. In General where we compare the monthly average for physical and chemical variation for Tigris water with standard world limits applied for grouping of surface water, we can consider the quality of Tigris water is good and suitable for uses as a source for drinking water by using traditional purification of water.While the results of study of Biological factors showed that the total number of bacteria for Tigris water was modularity clear and unsafe for drinking with out treatment include sterilization. High number of bacteria was found in station (1) during February 2008 when it reached (284) cell / ml and the lowest average recorded in station (5) at December 2007 when its reached (50) cell/ml. This study also shown that high number of diatoms were recorded in station (1) when its reached (46860) cell/L at December 2007 and the lowest number recorded in station (5) when it reached (236) cell / L at November 2007. It has been identifies (11) genus and sp. of diatom in this stations and there were :

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

اسم المؤلف: شيماء عبد محمد علي

اسم المشرف: وعد محمود رؤوف خالد عمر محمد علي

الموضوع العام: علوم الحياة

السنة: 2010

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

تقييم بعض المؤشرات المـناعية للتفاعلات الالتهابيـة المتـرافقـة مـع الاصابة بطفيلي الاكياس المائية Evaluation of Some Immunological Parameters Associated With Echinococcus Grunulosus Infection

اسم المؤلف: حارث برع حسن علي الاوسي

اسم المشرف: نغم ياسين كاظم البياتي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: علوم الحياة

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية في محافظة ديالى وشملت الدراسة 184 عينة من الاشخاص الاكثر تعرضا للاصابة بمرض الاكياس المائية. وبعد الكشف تبين ان 16 منهم تاكدت اصابتهم بالاكياس المائية في كل من الكبد والرئتين (12, 4 مريضا على التوالي) وقد تم تسجيل بعض المعلومات من كل | The present study was carried out in Diyala province, and included 184 samples at risk of infection with hydatid cysts. The diagnosis obtained that there were 16 infectied individual with hydatid cysts in liver and lung (12, 4 patients, respectively). Some information were recorded for each individuals. The study included 24 healthy individuals as control. Immunological test was carried out for each group. The results obtained that the rate of infection was higher in female (4.9%) compared with male (3.8%). The highest rate of infection was in 31 - 40 and 41 - 50 age groups and there was no significant difference among age groups. According to occupation there was no significant difference and the student shepherds were not infected with hydatid cyst. When investigation of risk factor, the result showed that (3.26%) of infected individuals were contact with animals while those who eat vegetables were higher (5.43%). The liver was the first infected organ according to distribution of infection in body follow by lung with 75% and 25% respectively and there was no significant difference. According to cytokines, IL - 4, IL - 17A, MIP - 1?, IP - 10, the study were higher in infected individuals compared with non infected and there was significant difference at 0.001 p.value. There was no significant difference in means of IL - 4 and MIP - 1? in both sexes but there was significant difference in IL - 17A and IP - 10 between males and females. The study showed that there was significant difference in IL - 4 according to location of infection and it was higher in lung compared with liver. No significant difference in IL - 17A, MIP - 1?, IP - 10 was shown according to location of infection

التحديد الجزيئي لبعض عوامل الضراوة في بكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة Molecular Identification of Some Virulence Factors In The Bacterium Klebsiella Spp Isolated From Different Clinical Sources

اسم المؤلف: فرقد حسن فليح

اسم المشرف: بهيجة عبيس حمود الخالدي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: جمعت (150) عينة من المرضى المراجعين والراقدين في مستشفى الديوانية التعليمي والذين يعانون من اصابات مختلفة وللفترة من 1/كانون الاول/2013 ولغاية 1/نيسان /2014 وتضمنت هذه العينات ماياتي : (50) عينة حروق وجروح، (50) عينة ادرار و(50)عينة قشع من المرضى المراجعين | One hundred fifty samples were collected from patients admitted in Al - Diwaniyah Teaching Hospital and suffering from various injuries and for the period from 1 / December / 2013 and up to 1 / April / 2014 these samples in clude : (50) sample burns and wounds, (50) urine and (50) sputum.The results showed that 90 samples (60%) of the total number gave is positive result for the growth.the bacterial isolates were distributed among 35 samples (70%) of burns and wounds, (33) the sample (66%) from urine and (22) sample (44%) from sputum After biochemical and cultural test found that 99 isolate belonging to Different Genus and species of bacteria these isolates include 40 isolate (44.44%) bound and wound isolates Staphylococcus sp was the predoniant in these samples by rate (42.5%), 36 isolate(40%) E.coli was most isolate by rate 50% in urine sample and 23 (25.5%) Klebsiella pneumonia was the most common isolate by rate (78.26%) in these putum.. Sensitivity test results of antibiotics showed that most of the isolates have high resistance against antibiotics used in the study and the most isolates possess multiple resistance (Cross resistance) as shown resistance to most of antibiotics that used in these study. All asolates (% 100) were resistant to nitrofurantin and Tetracycline while(95%)is resistant to ampiciline (90%) of isolate were resistant to thired generation of cephalosporins (ciprofloxacin and ceftazidime) and (%80) of isolate wereresistant to gentamicin and impeneme, finely (%80)of isolate were resistant to azetroname..The results of the molecular detection of genes uge and mag A gene in Klebsiella sp in the 16 (50%) of the isolates had mag A as the 8 (44.44%) of the isolates bacteria K.pneumonia carrier of mag A and 8 isolates of type K.oxytoca had of the gene above the gene uge was his appearance in the proportion of isolates (46.87%) as it appeared in 9 (50%) of the isolates K.pneumonia and 6 isolates (42.87%) of the isolates k.oxytoca..There were relationship between resistance of bacteria to antibiotics and possession of these genes

تقييم التعبير الجيني للجين UGT1A1 لدى حديثي الولادة المصابين باليرقان Assessment of UGT1A1 Gene Expression In Hyperbilirubinemic Neonates

اسم المؤلف: حسين حميد حسن

اسم المشرف: ظافرة جعفر عبد علي الفتلاوي صباح نعمة محمد الفتلاوي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان - الوراثة

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: استخدمت في هذه الدراسة 125 عينة من حديثي الولادة لتقييم التعبير الجيني لل UGT1A1 والكشف عن المتغاير UGT1A1*28. استخدمت 85 عينة منها في تقييم التعبير الجيني لل UGT1A1, بينما استخدمت ال 40 عينة المتبقية للكشف عن المتغاير UGT1A1*28. اجريت الدراسة الحالية في | A cohort of 125 neonates enrolled in the present study 85 of which subjected to the UGT1A1 expression analysis and forty (40) independent subjects examined for UGT1A1*28 variant. The project was performed in the laboratory of molecular genetics in the collage of education for women, TSB, UCB, BG and ELISA were performed in ATHOP, Assader teaching hospital and Annajaf private laboratory in the period from may to October 2013. Analysis of the UGT1A1 gene expression showed considerable decrease in UGT1A1 expression with relative risk 1.46. Analysis of regression of UGT1A1 mean against severity showed significant inverse correlation between severity of NH and mean UGT1A1 level (r = - 0.99, p = 0.03). Results showed that UGT1A1 expression in males significantly lower than females. Analysis of UGT1A1*28 showed that 80% of the hyperbilirubinemic neonates were positive while 20% were UGT1A1*28 negative. Concluding that there are a considerable proportion of neonates in our community not expressing UGT1A1 enzyme; therefore, they are a risk group for kernicterus. And must be registered and followed up because they are at risk of carcinogenicity, therefore, irinotecan (an anti cancer drug) and similar compounds toxicity. Expression of UGT1A1gene is higher in female than in male neonates, while neonatal jaundice is less severe in female than in male neonates. Eighty percent of jaundiced neonates are UGT1A1*28 mutants. Defective UGT1A1 is the main underlying cause of NJ in our community.

التحري عن عدد من المعادن الثقيلة والتلوث البكتيري في بعض الخضروات المعلبة والطازجة المستوردة في مدينة بغداد Determination of Several Heavy Metals And Bacterial Contamination In Some Imported Canned And Fresh Vegetables In Baghdad

اسم المؤلف: حسين خالد نعمان

اسم المشرف: ايثار كامل الميالي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: البيئة - التلوث البيئي

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: تم اخذ ستة وثلاثين عينة من الخضروات المعلبة وخمس عينات من الخضروات الطازجة, وتجميعها من مناطق واسواق مختلفة من مدينة بغداد بشكل عشوائي خلال الفترة من شهر تشرين الاول 2013 الى شهر نيسان, 2014 حيث تضمنت الدراسة التحري عن تراكير بعض العناصر الثقيلة وهي (الرص | In this study, thirty six samples of canned vegetables and five samples of fresh vegetables were collected randomly from different markets of Baghdad city from October 2013 till April 2014. The study included determining of the concentration of some heavy metals (lead, nickel, zinc and iron) by atomic absorption spectrophotometery and the bacterial contamination in samples, also comparing the canned vegetables with fresh vegetables. It was found that the higher concentrations of heavy metals in canned vegetables as the following : lead 1.179 µg/g in olive, nickel 0.908 µg/g in olive, zinc 10.143 µg/g green pea and iron 90.601µg/g in white asparagus. The lowest concentrations were as the following : lead 0.002 µg/g in green asparagus, nickel 0.019 µg/g in mushroom, zinc 0.528 µg/g in white asparagus and iron 4.061 µg/g in green pea. In fresh vegetables, it was found that the highest concentrations of heavy metals were as follows : lead 0.919 µg/g in green pea, nickel 1.108 µg/g in green pea, zinc 4.304 µg/g in mushroom and iron 43.618 µg/g in tomato. The lowest concentrations were as the following : lead recorded 0.011 µg/g in tomato, nickel recorded 0.022 µg/g in tomato, zinc 0.876 µg/g in green pea and iron recorded 11.081 µg/g in green pea. The identification of the bacteria done by some identification tests for gram negative bacteria, the testes include growth on MacConkey agar, growth on EMB agar, IMViC testes and motility test. The identification tests for Gram positive bacteria included growth on manitol salt agar, growth on staph 110 agar and motility test. The bacterial tests ensured that there is no bacterial growth was detected in the canned vegetables, except some samples (white asparagus, green asparagus and mushroom) of December 2013, while it was detected in fresh vegetables. The bacterial species that isolated in these samples were E.coli, Klebsiela pneumoniae, Bacillus cereus and Staphylococcus aureus.

التغيرات النسيجية في المشايم والاجنة نتيجة الاصابة بداء المقوسات في النساء المفحوصات بقياس الIgG Placental And Fetal Tissue Structural Changes Resulting From Congenital Toxoplasmosis In Women Diagnosed By IgG

اسم المؤلف: حلا ياسين كاظم

اسم المشرف: طالب جواد كاظم نغم ياسين البياتي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان - الانسجة

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: طفيلي المقوس الكونديToxoplasma gondii هو طفيلي ابتدائي اجباري داخل الخلايا ذات النواة. له القابلية على اصابة جميع حيوانات الدم الحار ويعد هذا طفيلي ذو اهمية صحية نظرا لما له من تاثيرات مرضية. لقد اجريت عدة دراسات بينت التغيرات النسيجية التي اثرت على انسجة | Toxoplasma gondii is an a zoonotic, obligate intracellular protozoan parasite that has the capacity to infect all warm - blooded animals. Many studies done to present the histological changes of the placental and fetal tissue and organs that resulted by Toxoplasmosis infection of pregnant women and about identification of infected fetal organs, but a lot of these studies were done experimentally on laboratory animals, accordingly the present study aims to study the structural changes of the placenta and fetal tissue and to identify the fetal organs in which the organism localized that taken from aborted fetus and embryos from pregnant woman that diagnosed as infected with Toxoplasma gondii. The present study was carried out during the period December 2012 to March 2013. Eighty women who had abortion chosen randomly from that which were revised gynecology theater in hospitals in Baquba city. Their age were ranged between 16 - 45 years, (10) fetuses and (33) samples of them non - infected as control group (c) and (37) samples were infected with Toxoplasmosis (I). The study included serological examination for mothers and Macropathlogical (Gross) , histopatholgical examination and Immunohistochimcal (IHC) stain of the placenta and fetal organs. Toxoplasmosis diagnosed serologically by ELISA (Enzyme Linked immune Sorrbant Assay) test. Immuno - histological techniques are used to detect the antigen and determination of their morphological localization in fetal and placental tissue. Formalin fixed paraffin embedded tissues (placenta and specimens of organs from aborted fetus) used to determined the antigen (Toxoplasma gondii) to detect the histological changes in these tissues. The ordinary histopathlogical changes of the tissue taken from aborted fetus showed different infiltration inflammatory cell and necrosis changes in brain, liver, lung, upper and lower limbs, kidney and spleen that may be due to infection of the parasite and not all infected organs localized the parasite (Tachyzoites). The results of the study showed that (46.26%) of the randomly selected mothers among that revised to the delivery theaters and had abortion. The fetal tissue and organs of the age (8 - 24) weeks gave positive (+) result for IHC in fetuses(brain and lung) and tachyzoites was detected in that organs, but fetus tissue of the age (4 - 8) weeks was negative for that test. Macropathlogical study of the placenta showed that the weight of the infected placenta was less than the normal ranges. and calcification was not significant in the infected placenta(45.7%) compared with non infected samples (4.28%). Infarction observed high significant in infected group was 25 (35.71%) compared with control group (2.85%) (p=0.001).Necrotic foci also seen as (40%) in infected group compared with control group (14.28%), differences between the two groups was statistically significant (p=0.001). Also congestion observed high significant (p=0.001) and the congestion was seen in (45.71) in infected group and (5.71%) in control group. Histopathlogical examination for placenta showed hemorrhage, necrosis, fibroniod necrosis and degeneration in trophoblastic cell, in both first and second trimester of pregnancy. Villous vascularity observed decreased as (32.85%) in infected group compared with control group (2.85%). Fibronoid necrosis in villi showed high percentage in villi which have > 3 that observed in infected group as (72.9%) compared with control (12.12%). An increased in villous stromal fibrosis was seen in (70.27%) placenta in infected group compared with control group which was (24.24%)

تاثير الاصابات المجهرية المهبلية وبعض الهرمونات على الاصابة بالمشعرات المهبلية عند النساء في بعقوبة - محافظة ديالى Effect of Microbial Vaginal Infections And Some Hormones On Trichomonas Vaginalis Infection In Women In Baquba - Diyala Province

اسم المؤلف: حنان رحيم حسوني الخشالي

اسم المشرف: نغم ياسين كاظم البياتي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: : توجد هناك عدة عوامل تلعب دور مهم في زيادة الاصابة بطفيلي المشعرات المهبلية وهذه العوامل تتضمن : اختلال مســـــتوى الهرمونات الجنســـــية, تغير الرقم الهيدروجيني للمهبل, بعض انواع الاصابات البكتيرية.الهدف من الدراسة : اولا : تحديد نسب وانواع الاصابات ال | Introduction : It have been founded that many factors play important roles in the increase incidence of infection with Trichomonas vaginalis these factors includes : disturbances in sex hormones level, change in pH of vagina and some types of bacterial infections.Aims : First : To determine the rates and types of Microbial vaginal infections that infected women in Baquba - Diyala province.Second : study of the relationship between the Trichomonas vaginalis and other microorganisms.Third : study the relationship between the Trichomonas vaginalis and the levels of some sex hormones.Patient and Methods : This study was carried out on 227 patients attended either Al - Batol Teaching Hospital for Maternity and Children in Baquba - Diyala, or some private clinic in Baquba city, most of these patient giving symptoms of vaginitis as a patient group, in addition to 10 healthy women as a control group.Patients group included married, widows and divorcees women with age range between (18 - 45) years, and at different educational level.Full clinical evaluation was done for those patient including history and physical examination. This evaluation revealed that 209 of patients (92%) had vaginal discharge, irritation and itching. Reguested blood samples were taken for laboratory test included estradiol, progesterone and testosterone. The level of these sex hormones in women with Trichomoniasis have been determined and compared with levels of these hormones in control group.In addition high vaginal swab were taken for microbiological tests. and used for diagnosis of Trichomonas vaginalis and other associated microorganisms, the rate of parasitic infection had been determined and it's relation with age group, marital status, level of education and level of vaginal acidity had been estimated. Moreover, the percentage of cases, with pus cells, epithelial cells, red blood cells and clue cells have been estimated. Results : the results of laboratory tests elicited the presence of Trichomonas vaginalis (6.3%). And in addition to it there is bacterial and fungal vaginal infection and the percentages were (54%) and (15%), respectively. Moreover, the results showed the presence of dual infections between the parasite and bacteria, the parasite and fungi, and bacteria and fungi and there percentages were (11.8 %), (8 %) and (4.9 %), respectively.Regarding age group, results showed that the highest parasite infection rate was among women in the age group (26 - 35 years) which was (41.1%), in compare with infection rate in others age group (18 - 25 years) and (36 - 45 years) which were (9.3 %) and (18 %), respectively. Regarding, the marited status, the highest infection rate was founded among married women in comparison with widows, divorcees women, and the percentages were (31.7 %), (6.8 %) and (14.7 %), respectively. Regarding, the educational level, the results showed highest rate of infection with Trichomonas vaginalis was among women with low educational level which was (11.8%) in compared with those with intermediate (7.9%), secondary (4.8%), and tertiary education (1.3%). Regarding, the pH of the vagina, results showed that the rate of vaginal infection were high when pH of vagina > 4.5 in comparison with the lowpH ? 4.5 and the percentages were (83.7%) and (16.3%), respectively. The results of direct smear and cultures showed that (25.9 %) of case (59 patient) were infected with Trichomonas vaginalis, (31.7%) of case infected with E.coli, (27.7%) of case infected with candida albicans, (21.1%) infected with Gardnerella vaginalis, (17.6%) percentages of Lactobacillus spp. (8.3%) percentages of Staphylococcus epidermidas (5.2%) in fected with S. aureus, (3.5%) infected with Enterobacter spp., (2.6%) infected with Proteus spp., (2.2%) infected with Klebsiella spp., (1.7%) infected with Acentobacter spp. and Streptococcus spp., (1.3%) infected with Pseudomonas spp., While the lowest rate was for Neisseria gonorrheae (0.8%). Also results showed that the highest percentage of dual infection with trichomoniasis it was in Gardnerella vaginalis which detected in 22 case (37.2%), followed by Candida albicans, which detected in 18 case (30.5%). While the lowest rate was with Neisseria gonorrheae which detected in one case (1.6%). The other laboratory tests including hormonal estimation in patients with trichomoniasis, the results of current study showed decrease level of estradiol hormone in women infected during follicular and luteal phases of the menstrual cycle, In follicular phase the mean of estradiol was (6.83 pg/ml) in comparison with control group that hormone rate was (42.61 pg/ml). In luteal phase the mean of estradiol was (27.00 pg/ml) comparison with control group which hormone rate was (87.16 pg/ml). In addition to that the level of progesterone have increased in follicular phase but decreased in luteal phase in comparison with control group. In follicular phase the mean of progesterone was (5.89 ng/ml) in com - parison with control group which hormone rate was (0.72 ng/ml). In luteal phase the mean of progesterone in infected women was (0.56 ng/ml) comparison with control group in which hormone rate was (19.83 ng/ml).Regarding, the level of testosterone, the results showed that the level had been increased during the follicular and luteal phases in infected women compared with control group. In follicular phase the mean of testosterone was (3.44 ng/ml) in comparison with control group which the mean was (0.24 ng/ml). In luteal phase the mean of testosterone was (3.65 ng/ml) in comparison with control group in which the mean was (0.30 ng/ml).Conclusion : The presence of infection with Trichomonas vaginalis, and it's associated with other microorganisms that are detected in the current study and other study previous may indicated a correlation between them. So those patient, constantly in need for a panel of laboratory test including : vaginal pH, and hormonal estimation as these factors may play a role in diagnosis and follow - up of the patients.

تاثير دوالي الخصيتين في معالم المني وسلامة الحامض النووي الـDNA Effect of Varicocele In Semen Quality And Nucleic Acid DNA Integrity

اسم المؤلف: حيدر لطيف فرحان المساعد

اسم المشرف: صاحب يحيى حسن المرشدي عبد الزهرة كاظم محمد علي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: شملت هذه الدراسة فحص 150 عينة من السائل المنوي لمرضى القيلة الدوالية Varicocele والاشخاص الخصبين بعد مدة امتناع قدرها 3 - 5 ايام حيث جمعت العينات في مختبرات مركز الخصوبة / مدينة الصدر الطبية / محافظة النجف الاشرف من 1 / 10 /2012 الى 30/ 4 /2013, لقلة الدر | This study was included 150 samples of semen for varicocele and fertile persons. The samples were collected after 3 - 5 day abstinence period at fertility center Laboratories / ALsader Medical city in An Najaf province.For the period from 1/10/2012 to 30/4/2013.Because the studies about varicocele are rare, the present studies aimed to know the impact of varicocele on semen and sperm parameter, sperm quality index, Malondialdehyde concentration and the percentage of sperm DNA integrity.So, this study was aimed at investigation wethere the Varicocelectomy in varicocele patients could positively influence studied parameters.The research was involved examining 83 sample of seminal fluid for varicocele patient. The percentage of varicocele grades were examined and the results were represented 40% grade1, 39% grade2 and 21% grade3.The result were showed a significant decrease (P<0.05) in sperm concentration , progressively motile sperm, normal sperm morphology percent and sperm viability percent for varicocele patient compared with the fertile persons, while there was noticed a significant increase (P<0.05) in Round cells concentration snd liquefaction time for varicocele patient compared to fertile person and the result of this study were showed significant decrease (P<0.05) of sperm quality index and sperm DNA integrity for varicocele patient compared to fertile persons (control).The study noted to the significant increase (P<0.05) of malondialdehyde concentration for varicocele patients compared to fertile persons. The coefficient factor was used to investigate the correlation between malondialdehyde concentration and the studies parameter.there was increase correlation (P<0.05) between malondialdehyde concentration and sperm concentration(r= - 0.86 P<0.05) progressively motile sperm (r= - 0.938), normal sperm morphology percent (r= - 0.90 P<0.05), sperm viability percent (r = - 0.846 P<0.05) and sperm quality index (r = - 0.836 P<0.05), while there was (P<0.05) positive correlation between malondialdehyde concentration and semen volume (r=+0.822 P<0.05), liquefaction time (r = +0.828 P<0.05) and round cells correlation (r= + 0.89 P<0.05).Also the coefficient was used to study the correlation between sperm DNA integrity and other study parameter. The results of this study were showed positive correlation between the percentage of DNA integrity and sperm concentration (r= +0.88 P<0.05), progressively motile sperm (r=+0.78P<0.05), normal sperm morphology (r= +0.86 P<0.05), sperm viability percentage (r=+0.87 P<0.05) and sperm quality index (r= +0.81 P<0.05), while it was noticed in used correlation between sperm DNA integrity and malondialdehyde concentration (r= - 0.681 P<0.05), semen volume (r= - 0.67 P<0.05), liquefaction time (r= - 0.65 P<0.05) and round cells (r= - 0.54 P<0.05). The effect of varicocele grading on sperm and semen parameter, sperm quality index, malondialdehyde concentration and percentage of sperm DNA integrity was studied, the result were showed a significant decrease (P<0.05)of sperm concentration, progressively motile sperm, sperm viability and normal sperm morphology percentage, and a significant increase (P<0.05) of liquefaction time and Round cells concentration for varicocele patients grad1, grade2 and grade3 compared with the fertile persons. and when we are compare between varicocele patient according to type of grade the results were show a significant decrease (P<0.05) of sperm concentration, progressively motile sperm and normal sperm morphology percentage and a significant increase (P<0.05) of liquefaction time for varicocele patients grade2 and grade3 compared to grade1 varicocele patient.So the result showed a significant decrease (P<0.05) of sperm concentration for grade3 varicocele patients compared with the grade2 varicocele patients. The result of this study were showed a significant decrease (P<0.05) in sperm quality index and sperm DNA integrity for grad1, grade2 and grade3 varicocele patients compared to fertile persons. also it was noticed a significant decrease (P<0.05) of sperm quality index and sperm DNA integrity for grade2 and grade3 varicocele patients compared to grade1 varicocele patients but the malondialdehyde concentration according to varicocele grade, the result were showed a significant increase (P<0.05) of malondialdehyde concentration for grade2 and grade3 varicocele patients compared to grade1 varicocele patients and fertile persons. In this study the effect of Varicocelectomy on study parameters was studied and the result were showed a significant increase (P<0.05) of sperm concentration, progressively motile sperm, sperm viability percentage, normal sperm morphology, percent, sperm quality index and sperm DNA integrity and a significant decrease (P<0.05) of liquefaction time and malondialdehyde concentration for Varicocelectomy compared to varicocele patients. The result were showed a significant decrease (P<0.05)of sperm viability percent, normal sperm morphology percent, sperm DNA integrity and sperm quality index and a significant increase (P<0.05) in round cells concentration, liquefaction time and malondialdehyde concentration for Varicocelectomy patients compared to fertile persons.From the result of present study, we are concluded that varicocele has been negatively impact on sperm and semen parameters, malondialdehyde concentration and sperm DNA integrity, and the effect of varicocele is associated with the increasing of varicocele grading, while Varicocelectomy has been positive impact on sperm and semen parameters, malondialdehyde concentration and sperm DNA integrity but this result have not reached to similar state to fertile persons

انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

اسم المؤلف: ذو الفقار حيدر حسن

اسم المشرف: ماجدة مالك متعب

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

العلاقة بين كروماتين النطف ومعايير المني في مرضى عديمي الخصوبة The Relationship Between Sperm Chromatin And Semen Parameters In Infertile Patients

اسم المؤلف: وليد عبد علي حسن الخفاف

اسم المشرف: صاحب يحيى حسن المرشدي زينب شنيور مهدي الطرفي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: هدفت الدراسة الحالية الى تقييم النسبة المئوية لكروماتين النطفة غير السوي Abnormal sperm chromatin percentage لمرضى عديمي الخصوبة مقارنة بالرجال الخصبين ودراسة العلاقة بين النسبة المئوية لكروماتين النطفة غير السوي ومعايير المني ومؤشر نوعية النطف وتركيز الم | The aim of the current study was to evaluate the Abnormal sperm chromatin percent for the infertile comparing with the fertile men and study the relation between the Abnormal sperm chromatin percent, the sperm and semen parameters, sperm quality index and MDA concentration for the infertile normozoospermic and asthenozoospermic patients comparing with the fertile men.This study was conducted in the laboratories of fertility centers in Al - Sader medical city/ Al - Najaf Al - Ashraf from 1 / 11 / 2012 to 30 / 4 / 2013.By the end of the semen liquefaction time, the 72 samples were divided into two groups : - the fertile men, (17), and the second is represented by the Infertile Normozoospermic (23) and the Asthenozoospermic (32). The age average was (1.36±30.9) , (0.98 ±29.6) and (1.63± 31.4) years respectively. The samples were restricted to the non smoking patients only. The study results show a significant (P< 0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the sperm viability percent and the semen volume, and a significant (P<0.05) increase in the round cell concentration, liquefaction time, Malondialdehyde concentration and the percent of the abnormal chromatin of the Normozoospermic and the Asthenozoospermic patients comparing with the fertile men. The results also show a negative correlation (P<0.05) between the (MDA) concentration and the parameters represented by the sperm concentration, percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent sperm quality index and semen volume. While it had been noticed that there was a positive relation between the MDA concentration and the parameters represented by the sperm liquefaction time, round cell concentration and the abnormal sperm chromatin percent of all the study groups. In this research the relation between the sperm chromatin damage and the other study parameters had been studied by dividing the samples according to the degree of chromatin damage. The results show a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent, the sperm quality index and semen volume, with a significant (P<0.05) increase in the liquefaction time and round cell concentration (MDA) concentration and the percent of the abnormal chromatin of the group (> 10) comparing with the group (? 10) for the fertile men, also the results show a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent and the semen volume, with a significant (P<0.05) increase in the liquefaction time and round cell concentration for the group (> 25) comparing with the group (? 25) for the infertile Normozoospermic patients. For the Asthenozoospermic patients the study shows a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm quality index, the sperm viability percent and semen volume, and a significant (P<0.05) increase in the liquefaction time and round cell concentration for the study groups represented by the group (> 20 - 40) and (< 40) comparing with (? 20). From the above results it could be concluded that the increase of (MDA) level resulted from the active (ROS) leads to an increase in the abnormal chromatin percent of the infertile Normozoospermic patients which had a negative effect on the semen parameters which could be a reason for the infertility for those patients

علاقة بعض الهرمونات والمعايير الكيموحيوية للمصابين بحصى المرارة Relation of Some Hormones And Biochemical Variables For Patients With Gallstones

اسم المؤلف: انسام فيصل ياسر الجوراني

اسم المشرف: احسان ريسان ابراهيم الركابي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الحيوان - الفسلجة

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: صممت الدراسة الحالية لمعرفة مستويات بعض الهرمونات والمتغيرات الكيموحيوية لممصابين حصى المرارة، اذ اجريت الدراسة على المرضى الوافدين الى مستشفى الزهراء التعليمي ومستشفى الكرامة التعليمي / قسم الجراحة في محافظة واسط للمدة منذ بداية شهر تشرين الاول (2012) حت | The study was Designed to investigate some hormonal levels and biochemical variables in patients with gallstones, where the study was conducted on patients attending Zahra Hospital and Karama Hospital / Department of Surgery in Wassit province for the period since the beginning of the month of October (2012) and until the end of the month of April (2013), The number of patients (75) were aged between 15 - 70 years and the number of males (15) and females (60) compared with healthy controls and numbered (25) aged between 15 - 70 years and the number of males (5) and females (20). Blood samples were collected in volume (10 ml) blood serum was isolated to achive required measurements, which included measuring the level of the hormone leptin, thyroxine (T4), thyroid stimulating hormone (TSH), growth hormone (GH), also the study included estimate the concentrations of biochemical parameters : total cholesterol (T.Chol), higher density lipoprotein cholesterol (H DL - Chol), factor risk (RF), liver enzymes ALT and AST, total serum bilirubin (TSB), glucose, creatinine, albumin, Globulin, and total serum protein (TSP), The results of the study were as follows : .Prevalence of gallstones in Wassit province, and Females injury rate will gallstone in wassit province much higher than males with rate of 5/1. Significant increase (P <0.05) in the levels of leptin hormone and GH as well as non significant increase (P >0.05) in the levels of the hormone TSH, while there was a significant decrease (P <0.05) in the level of the hormone T4 in people who suffer from a gallstones disease compared with healthy people. Significantly increase (P <0.05) in the concentration of T.Chol, and RF, while there a non - significant decrease (P> 0.05) in the concentration of HDL - Chol. significant increase (P <0.05) in the concentration of ALT, AST, TSB, and glucose with a non - significant increase (P>0.05) in the concentration of createnine. significant decrease (P <0.05) in the concentration of albumin, TSP, while an increase is not significant (P>0.05) in the concentration of globulin. There is effect of sex, age, BMI, and duration of the disease for patients with gallstones compared to healthy persons. There is effect of the taking contraceptive pills for female patients infected with gallstones compared to patients female non Taking contraceptive pills.

دراسة مناعية نسجية للاستجابة المناعية لسرطان الغدة اللبنية المغروسة في الفئران البيض Immunological And Histological Study For Immune Response To Implanted Murine Mammary Adenocarcinoma In Albino Mice

اسم المؤلف: نور ابراهيم عبد الزهرة شبر

اسم المشرف: احمد حميد عبود العزام جميل جري يوسف الحميداوي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية - المناعة

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: يعد السرطان واحدا من المخاطر الاساسية التي تهدد حياة الانسان في مختلف بلدان العالم، لان هذا المرض لا يقف عند عضو معين فهو ينتشر الى كثير من اعضاء الجسم الاخرى ليفتك بها، ومن هذا انطلقت الدراسة الحالية التي اجـريت للفترة من تشرين الاول / 2012 م ولغاية اب | The Cancer is one of highly risk factors that threats the human life world wide, because the disease has metastatic property from primary to secondary sites. This study was carried out during the period extending between October 2012 and August 2013, in laboratories of Faculty of Veterinary Medicine & Faculty of Education for girls in University of Kufa. It is aimed to follow up the changes in volume of growing tumor in female albino mice which were injected with cells of murine mammary adenocarcinoma.The histological changes, immunohistochemical examination investigate the distribution of CD8+ T lymphocytes were also studied.The changes of IgG and IgM titers in serum were also investigated. The study included use of 40 female albino mice at age 6 - 8 weeks, 25g weight average.All mice were injected by cells of murine mammary adenocarcinoman (AM3) in subcutaneous of femoro - dorsal region, and ascending to cervical region. The tumor was successfully implanted into 28 mice.These mice divided into four groups, each contains seven tumor - bearing mice.The animals of the first group subjected to the investigations after 48 hrs from implantation , while those of 2nd, 3rd & 4th groups after 10, 20, 30 days respectively. Seven healthy mice were considered as control group to comparison of immunological tests. Tumor volume & relative tumor volume were measured in different experimental groups. Histological section were prepared by routine histological techniques that including the steps of dehydration, clearing, infiltration, embedding, cutting & staining by hematoxylin and eosin stains, then microscopical examination had been done by optic microscop. The results indicated there was significant increase in tumor volume and relative volume with advancing of tumor age in animals of 2nd, 3rd & 4th groups where compared with first group. The gross examination of the animals of different groups showed that the tumor grew as irregular projection with loss of hair and erosion of overlying skin with milky discharge. The post - mortem examination revealed that the tumor mass was divided into small lobules with various coloures and the tumor mass surrounded by fibrous tissue which increase in thickness as the tumor increased in the age. Extensive areas of necrosis were noticed in the centers of the tumor masses. The microscopic examination of tumor masses in the first group raveled there were sub cataneous small aggregation of tumor cells at the implantation region as well as dispersion of the implantated tumor cells. The inflammatory cells, especially neutrophils were infiltrated. In the second group, the tumor masses showed lobulation by fibrous septa with proliferation of new blood vessels with congestion of them. Infiltaration of inflammatory cells, particularly the lymphocytes. The tumor cells arranged as ribbons and acini, characterized by hyperchromacia pleomorphism, high N/C ratio as well as appearance of mitotic figures. The histological features of the third group revealed increase of amount of fibrous tissue extensive necrotic areas and loss of architecture.These appeared in the fourth group in addition to the presence of cellular debris with dead inflammatory cells. The immunohistochemical study showed no CD8+ T cells were infiltrated in the mass of first group but the little infiltration of these cells was show in the second group. The highest infiltration of the CD8+ T cells was appeared in the third group. while the little cells were discharged in the fourth group. The results of the single radial immunodiffussion assay showed that the implantation with mammary adenocarcinoma had significant effect in increasing the titer of IgM after (48) hours and (30) days from implantation of tumor compared to control group, while the titer is not significant after (10) and (20) days from implantation of the tumor compared to control group. Also the results revealed a significant decrease in the titer of IgG after (48) hours and (30) days from implantation of the tumor, while were no significant increase in the titer of immunoglobulin after (10) and (20) days from implantation of the tumor, compared to the control group. From all the above, We concluded that there was variable histological changes as the age of tumor advanced these changes were characterized by increase of amount of tumor cells in the first groups and occurrence of necrosis in late groups, as well as increase the fibrous tissue as the tumor age advanced. The infiltrative CD+8 lymphocyt was also variable as change of tumor age. The implantation of tumor in mice groups had marked effect in stimulation of humoral immune response through increase of IgM & IgG titers.

التعبير الجيني للمورث Erg11 وعلاقته بالمقاومة وظاهرة رجوع النمو لبعض عزلات خميرة المبيضات البيضاء المختبرة تجاه مضادات الازول الفطرية Gene Expression of Erg11 And It’s Relation With Resistance And Trailing Growth Phenomena of Some Isolates of Candida Albicans Tested Against Azole Antifungals

اسم المؤلف: حيدر عبد الحسين عباس

اسم المشرف: عدنان حمد عبيد الحمداني

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: هدفت الدراسة الحالية الى الكشف عن دور المورث Erg11 والتعبير الجيني النسبي له في مقاومة خميرة المبيضات البيضاء C.albicans وعلاقته بنشوء عزلات مقاومة او ظاهرة رجوع النمو Trailing growth لهذه الخميرة عند اختبار حساسيتها الدوائية تجاه بعض مضادات الازول (الفلو | The current study aimed to detect the role of Erg11 gene and its relative expression in the resistance of Candida albicans, and its relationship with the emergence of resistace or Trailing growth isolates when tested its susceptibility against azoles agenst (fluconazole, ketoconazole). A total of 120 specimens were collected from patients of both gender with different ages whom infected with oral thrush, uro - genital and Gastrointestinal candidiasis and admitted the General Teaching Hospital and materinaty and children Teaching Hospital in AL - Diwaniyah city during the period from December/2012 to February / 2013. The isolation and identification of yeast isolates were done based on their morphological, Cultural and biochemical tests in addition to use the confirmatory systems such as the growth on Chrom Agar candida, Api Candida and Tobacco Agar medium to distinguish C.albicans from other yeast spp. Also, some virulence factors of C.albicans such germ tube formation, chlamydospores production, growth at of 45 c and resistance to cycloheximide were detected. The antibiotics sensitivity test was used in two method (disk diffusion & dilution) to determine the sensitive, trailing growth, resistance isolates and minimal inhibitory concentration (MICs) toward azole agents. The qRT - PCR was used to detect the relative quantitative of Erg11 gene expression and its relationship with resistance to azole and emergence of trailing growth phenomena in the presence of Fluconazoles and ketoconazole. The isolation and identification results revealed that the isolation percent of C.albicans was 47.05 % incomparison with other Candida species that were 15.3%, 11.76 %, 8.23 % and 5.9 % for C.krusi, C.dubliniensis, C.tropicalis, C. parapsilasis and C.glabrata , respectively. The antibiotic susceptibity tests of C.albicans showed the presence of resistance precent (38.89%) to fluconazole and the most of sensitive isolates revealed a trailing growth phenomena in the zone of growth inhibition of floconazole in a ratio 55.56%. While the resistance percent to ketoconazole was 27.78 % and the sensitive isolates showed the trailing growth in a ratio (38.89%). The statistical analysis showed a significant differences (P <0.05) among tested treatments. The MICs values of fluconazole against C.albicans isolates were 8, 16, and 64 Mg/ml for sensitive isolates and were 0.125 and 0.5 Mg /ml for resistance isolates and the trailing growth phenomena occurred at MICs value ? 8 Mg/ml within 24 hours of incubation while the values became ? 64 Mg/ml after 48 hours of incubation.There was a significant differences (p< 0.05) among tested treatments isolates incubated at 24 hours while there was no significant differences (P>0.05) at 48 hours of incubation. In respect with Ketoconazole, the resistance of C.albicans was increased after 48 hours of incubation at MICs values 0.125, 0.25, 0.5 and 1 Mg /ml while its was sensitive at values 4, 8 and 16 Mg /ml and the trailing growth phenomena occurred when the MICs ? 2 Mg/ml after 24 hrs of incubation, while it reached to ? 8 Mg/ml after 48 hrs of incubation. There was a significant differences (p< 0.05) among tested treatments incubated after 24 hours while there was no significant differences (P >0.05) among treatments after incubation 48 hours. The results of relative quantitative gene expression for both genes revealed a significant differences (p< 0.05) in gene expression levels through the occurrence of increased in the transcription of mRNA quantity among tested treatments.The up - regulation of Erg11 and fold change in mRNA transcription was 2.498 fold compared with 1.0 for control treatment after 24 hrs of incubation in the presence of antifungal, while the increasment was 12.606 fold after 48 hrs of incubation of isolates with antifungals.

دراسة بعض الصفات الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت Study of Some Physical, Chemical And Biological Properties on Tigris River in Tikrit City

اسم المؤلف: بشار طارق اسماعيل هلال الشنداح

اسم المشرف: رياض عباس عبد الجبار

الموضوع العام: علوم الحياة

السنة: 2008

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: تهدف الدراسة الحالية للتعرف على بعض الجوانب الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت ولمياه المخلفات السكنية في محطة المعالجة.جمعت العينات لمدة سبعة اشهر اعتبارا من شهر تشرين الثاني 2006 وحتى نهاية شهر ايار 2007 وبمعدل مرتين شهريا | The present study aimed to find out some physical, chemical and biological aspects of Tigris river passed in Tikrit - city, and population residence in the treatment unit.Samples were collected over the period of seven months during November 2006 to the end of May 2007 biweekly. Four stations were selected for sample collections over the distance of 25 km. Two stations were in Tigris river and two were in the treatment unit. The first selected station was close to the old water purification unit in Tikrit - city. The second station was selected at the treatment unit in AL - Dubaee before the mechanical treatment unit. The third station was selected at the treatment unit after the biological treatment. The fourth station was selected in Tigris river about ten km south of the treatment unit at AL - Dijla town. The study involved an investigation of some physical, chemical and biological aspects of Tigris river and the residence waste discharges to the river. This included air and water temperature, conductivity, PH, dissolved oxygen, BOD, Turbidity, total hardness, calcium and magnesium hardness, chloride, total alkalinity, nutrients (i.e., phosphate, nitrite, silica) , and the concentrations of heavy metals (i.e., cadmium and cupper). The biological factor included the total bacteria counts cultured on nutrient agar. Results of the study clarify the close relation between air and water temperature which varied (8.5 - 36) C0 and (9 - 27) C0 respectively. Conductivity values were relatively high and ranged (128 - 3530) microsemens/cm. River water almost was alkaline during the period of the study. PH values of the first and fourth stations were close in the river, although a value of 6.12 was recovered at station on during May. In all stations, PH values ranged (8.8 - 6.12). River water has a good ventilation, dissolved oxygen values ranges (8 - 11) mg/L at the first and the fourth stations, while these values were (0.1 - 1.34) mg/L at the second and third stations. Mean dissolved oxygen values pointed out the availability of good oxygen concentration for the continuity of living organisms which ranged (0.3 - 5.8) mg/L in Tigris river, while BOD values at the stations second and third were high and ranged (5.6 - 131.2) mg/L. Turbidity was high during April at the stations one and four and ranged (2.03 - 788) NTU in all stations. Total hardness in Tigris river was slightly low - very hardness and ranged (120 - 360) mg/L at the first and fourth stations, while these values were (180 - 480) mg/L at stations two and three.The high total hardness values coincided with the high conductivity values. Concentrations of calcium ions responsible on the calcium hardness ranged (211.4 - 120.7) mg/ L was higher than magnesium ions responsible for magnesium hardness range (5 - 265) mg/L.Records of chloride ions ranged (14.16 - 169.99) mg/L at all stations. However, chloride values at stations first and fourth were among the allowed world values and ranged (14.16 - 42.48) mg/L.Alkalinity values were due to the carbonate and bicarbonate and ranged (35 - 725) mg/L.phosphate values ranged (0.168 - 25.2) µg phosphor atom - phosphate/L. Low nutrient values recorded in Tigris river, while the highest values recorded at second station (treatment unit).Nitrite values fluctuated at all stations and ranged (0.078 - 14.778) µg Nitrogen atom - Nitrite /L. Silica values ranged (648 - 5961.6) µg silicon atom - silica /L. Heavy metals values (Cadmium and Cupper) clarify that, Cadmium were (un sensible), except one value (0.008) mg/L that recorded at November, and un sensible for Cupper.Results of the statistical analyses of all involved factors showed significant differences at stations first and second, and between the first and third stations as well as the fourth station. While there was no significant differences between the second and fourth stations. And no significant differences between stations first and fourth.Total bacterial counts indicated high bacterial counts, and the average of total bacterial count ranged (2.605X105, 5.44X108, 2.265X108, 2.385X105) / ml at the four stations respectively, so the Tigris river considered pollutant - very pollutant.