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دراســــة الفعالية التضادية لجرثومة Bifidobacterium spp تجاه جرثومة Staphylococcus aureus المقاومة للمثيسلين == Studying The Antagonistic Activity of Bacteria Bifidobacterium Spp Against Bacteria Methicillin Resistance Staphylococcus Aureus
Author name:
انغام نجاح هادي الخفاجي
Supervisor name:
سهام جاسم الكعبي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
24T3009 - p.pdf
Abstract:
تضمنت هذه الدراسة التحري عن الفعالية التضادية لجرثومة Bifidobacterium spp تجاه عزلات سريرية لجرثومة Staphylococcus aureus المقاومة للمثيسلين، ودراسة تاثير تلك المواد على عوامل الضراوة المنتجة من قبل S.aureus المقاومة للمثيسلين مثل الغشاء الحيوي Biofilm وا | This study included the investigation of Bifidobacterium spp antagonistic activity against Staphylococcus aureus isolates, and the effect of these antimicrobial agent on virulence factors produce by MRSA (Methicillin Resistance Staphylococcus aureus) such as Biofilm and Slime layer. One handered different samples were collected to isolate Bifidobacterium spp and 50 clinical isolates of Staphylococcus aureus from clinical specimens in AL - Najaf AL - Asharaf governorate during the period of (10/10/2013 - 20/1/2014) by the cultural characteristic colonies, microscopic for cells, biochemical tests and polymerase chain reaction PCR technique to detect 16SrDNA, the results showed that 13 isolates belong to Bifidobacterium spp and there are divergent gene content between these isolates and they have been bearing lm26/ lm3 gene. Carbohydrate fermentation test was used to distinguish the species of Bifidobacterium, the results revealed that the B.bifidum is more predominant than the other species; B.thermoacidophilum, B.adolescentis, B.breve, B.longum. Antibiotics susceptibility test to Staphylococcus aureus using antibiotic disc diffusion assay showed that Methicillin Resistant Staphylococcus aureus formed 70% and 30% of isolates were sensitive to the same antibiotics, also the ability of MRSA isolates to produce Biofilm and Slime layer was investigated by tube method and Congo red agar, then 10 isolates of Staphylococcus aureus were selected as efficient isolates according to the resistance of Methicillin and the high ability to produce biofilm and Slime layer. The antagonistic activity of Bifidobacterium spp was extracted and tested against these MRSA isolates, the results showed that the most isolates of Bifidobacterium spp were effective against Methicillin Resistant Staphylococcus aureus (MRSA), as well as the inhibition of growth and formation of Slime layer by using the wells and disc diffusion agar assay. The inhibition zone was between (10 - 30)mm, and the ability of bacteriocin production of Bifidobacterium spp against MRSA have been conducted by well diffusion broth method, B.bifidum3 was greater than other species in producing bacteriocin with asignificant increase (P<0.05). Furthermore the impact of bacteriocin production to inhibit the development and formation of biofilm was investigated, the results showed that the bacteriocin has ahigh activity against MRSA. The bacteriocidal and bacteriostatic effect of bacteriocin was conducted by using spectrophotometer, the results showed that these effects were bacteriostatic in the beginning of the growth MRSA while it was lethal during other time and 40 minutes is the period needed for killing MRSA bacteria.
