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تقييم كفاءة بعض المستخلصات النباتية وعقار الميترونيدازول Metronidazole في حيوية الاميبا الحالة للنسيج Entamoeba histolytica خارج الجسم الحي In vitro مع دراسة جزيئية لتحديد بعض الانواع التابعة لها في محافظة بابل == Evaluation of Some Plant Extracts And Metronidazole Drug In Vitro of Entamoeba Histolytica Viability And Molecular Study For Some Species Determination In Babylon Province
Author name:
سحر عباس حسين المعموري
Supervisor name:
هادي مزعل خضير الربيعي | احمد خضير عبيس الحميري
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Babylon - College Of Science For Girls - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T2985 - p.pdf
Abstract:
اجريت الدراسة الحالية للمدة من تشرين الاول 2013 ولغاية تموز 2014 في كلية العلوم للبنات / جامعة بابل, اذ تم الحصول على طفيلي الاميبا Entamoeba من عينات المرضى المصابين والمراجعين لمستشفيات بابل للاطفال والولادة ومستشفى ابن سيف للاطفال والمركز الصحي الحسين | The present study was conducted during the period from October 2013 till July 2014 in the College of Science for women, Babylon University, the Entamoeba stool samples were collected from patients that attending to Babylon Maternity and pediatric hospital, Ebn - Saiff hospital and Abi - Gharaq and Al - Mussaib of primary health center, Babylon province. The parasites were detected by direct smear method, 130 stool samples were collected that contain blood and mucus. The overall percentage incidence of E. histolytica (80.8%). No significance differences of infection rates in males and females and the highest rate of infection was in (1 - 10) year age group for males and females (82.9% and 39.1% respectively) while the lowest infection rate was in (51 year and more) for males and females (33.3 %, 6.7 % and respectively).Three plants extracts (cooled and Boiled) water (Euphorbia helioscobia, Eucalyptus gloubules and Mytrus comminus) evaluated at the culture media (Beef liver infusion media) that grown with E. histolytica. The results revealed the E. helioscobia boiled water extracts the efficient extracts to killed the all parasites in the second day from experiment beginning with 10 mg/ ml concentration and the next efficient plant extracts (boiled water) its E. globules and M. commines was the third day with 5 mg / ml concentration. The Metronidazole showed to killed all parasites in the third day with 1 microliter / ml. and from this present study showed that the successful growth of E. histolytica in culture media for some day and finished (Killed) at the fiveth day from experimental beginning.Isolation and identification of some terpenoid compounds were detecting in the present study belong E. helioscobia plant extracts (Camphene, ???pinene, Euphornin, ?? caryophyllene, Myrecene and ??humulene) by (HPLC).Molecular study was conducted to determined three species of Entamoeba by using Polymerase chain reaction for ten stool samples that primarily detected with Amoebic dysentery by direct smear method and the following results for molecular study, the highest overall percentages its E. dispar with (7/10) mean 70% whereas the E. histolytica and E. moshkoviskii its (6/10) mean 60 % for each one, as well as existence the double infection or tertian infection with these species for Entamoeba.
