انتشار داء اللشمانية الجلدية في محافظة القادسية مع تقييم مدى الاسجابة للعلاج بعقار البنتوستام بطريقة RT - PCR == Prevalence of Cutaneous Leishmaniasis In Al - Qadissia Province And The Evaluation of Treatment Response By Pentostam With Rt - Pcr
Author name:
رنا صالح صاحب محلول الدفاعي
Supervisor name:
غيداء عباس جاسم الجبوري
General topic:
Biology
Specific topic:
Microbiology - Parasites
Degree:
Master
University:
University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Qadisiyah
First pages:
24T2937 - p.pdf
Abstract:
Results of clinical and laboratory examination of 239 cases showed 55 infection cases in Cutaneous Leishmaniasis and in infection proportion reaches at 23.01% out of the total number of the Cutaneous cases which are similar to Leishmaniasis during the months of the study. The Results also showed that the highest infection was recorded between the ages 1 - 10 years old and about 26 cases in 47.27% and between 10 - 20 years old about 17 cases in 30.9% and the lowerest was between 40 - 50 and 70 - 80 years old in one cases in 1.8%. The summit of the disease spread was in February for about 24 cases in 43.63% specially in AL - Hamza AL - Sharqi where the highest number of infection was recorded for about 23 cases in 41.81%. It is followed by AL - Shafiya for 10 cases in 18.18% and the lowerest was AL - Mhannawiya for one case in 1.8%. It has been clear that the number of the multiple epidemics is more than the individual ones in 70.5% while the latter in 29.98% respectively. It was focused on hands and legs in 50.35% and 24.46 %. The tissue test showed thick perspiration in the big infected pharynx and lymphatic cell in addition to the existence of abscess to the smash of the infected cell. Forty nine positive cases were found in 89.09% for the genetic material DNA which is connected with Leishmaniasis by mean of NestedPCR to identify the type results showed 47 samples which is L. major in 95.91% and to get sure by investigating the virulence factor lipophosphoglycan, proteophosphoglycan and cysteine protease it was found that all sample were positive in 100% as Leishmania. major. To know the affect of treatment pentostam on these samples, the standard proportion for the genetic patterns was calculated factor lipophosphoglycan, proteophosphoglycan and cysteine protease during five week. Results showed that there was a big difference for each gene in a solation during the period of treatment in a level of the possibility of (P?0.05) when used test LSD. and by comparing the three genes it was found that there was a meaningful between cysteine protease and the other two factor lipophosphoglycan, proteophosphoglycan and there wasn’t difference between lipophosphoglycan, proteophosphoglycan. Hence, it has been clear that the treatment pentostam has a big affect in parasite by its affect on the virulence factors and this explains that possibility of infection may happen again after having the treatment. The skin disease cutaneous Leishmaniasis is considered as one of the endemic parasitic diseases in Iraq. This study aims at identifying its prevalence and specifying the virulence factors in Cutaneous Leishmaniasis in order to define the type of disease reason and the affect of the treatment of Pentostam on the virulence factors lipophosphoglycan, Cysteine protease, proteophosphoglycans by means Real time - PCR
