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تاثير الجسيمات النانوية للنحاس على بعض اعضاء الجهاز التكاثري الذكري للفئران البيض Mus musculus == Effect of Copper Nanoparticles On Some Organs of Male Reproductive System In Albino Mice Mus Musculus
Author name:
ايمن راضي حبيب
Supervisor name:
محمد ناجي طه
General topic:
Biology
Specific topic:
Zoology - Physiology
Degree:
Master
University:
University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Baghdad
First pages:
24T2908 - p.pdf
Abstract:
This study was conducted on 20 male of Swiss albino mice, where the main target of this study was to identify the influence of copper nanoparticles (Cu - NPs) on some organs of the male reproductive system. Animals were orally administered with 0.1 ml of 100 mg/kg Cu - NPs for 7, 14, 21 days. Then the animals were dissected in the next day after the end of the dosing period and took from them testes and epididymis (head and tail) to study the parameters in which these particles have affected, and this parameter as follows : 1 - Changes in the weights of each of the animals bodies, testes, tunica albuginea, and epididymis (heads and tails). 2 - Changes in sperm characteristics in testes and epididymis (head and tail), which included : the percentage of live sperms, abnormal sperms and sperm concentration. 3 - Changes in the percentages of the spermatogenic cells, which included : a - Spermatogonia. b - Primary & Secondary Spermatocytes. c - Spermatids. d - Sperms. 4 - Changes in numbers of leydig cells and nuclei diameters. 5 - Changes in diameters of seminiferous tubules and their lining wall thickness. 6 - Changes in thickness and height of epithelial cells in both the head and tail of the epididymis. 7 - Histopathological changes in testes and epididymis (head and tail). Phenotype description of testes from mice treated with copper nanoparticles for a period of 21 days occurrence of congestion and a few amount of fatty material around the testes. The results also revealed a decline in the average body weights on the period of 7 and 21 days of treatment when compared with the weight of the same animals before treatment and the decline was significantly at (P<0.05). Whereas the testes and tunica albuginea weights recorded a significant decrease in their average weights (P<0.05) when compared with control groups. As well as the epididymis weights where showed significantly decrease (P<0.05) in the average of the epididymides heads weights for all three periods of treatment, while a significant decrease (P<0.05) in the average weight of the epididymis tail appear for two periods 7 and 21 days compared with control groups. The study also reported a decrease in the percentage of live sperm and the average sperm concentration and an increase in the percentage of sperm abnormalities extended to all periods of treatment compared with control groups and this change was significant at the level of (P<0.05). The number of leydig cells and nuclei diameters, the results show a decrease in their averages and all the extended treatment period when compared with control groups, and this decrease was significantly at (P<0.05). Treatment with particles of copper nanoparticle indicated a decrease in the percentage of spermatogonia, primary spermatocytes and sperms in all periods of treatment compared with control groups and this decrease was significantly at the level of (P<0.05). While the results of the study revealed a significant increase at the level of (P<0.05) in the percentage of secondary spermatocytes and spermatids compared with the control animals. The study also showed a significant decrease at the level of (P<0.05) in the diameter of the seminiferous tubules of testes and the wall thickness of the seminiferous tubules for all periods of treatment compared with control groups. As well as a reduction in wall thickness and height of the epithelium in epididymis (head and tail) for all periods of treatment compared with control animals and this decline was significantly at (P<0.05). The current study also recorded a textile damage in the testes and epididymis (head and tail), which summarized the crash of some seminiferous tubules, degeneration of lining cells in tubule, the appearance of vacuole between cells and lifting epithelium from the basement membrane. On this basis, this study has shown for the first time that particles of copper nanoparticles have a negative impact on the effectiveness and activity of the male reproductive system in albino mice.
