الاكثار الخضري لنبات Spilanthes acmella (L.) Murr. خارج الجسم الحي == In Vitro Vegetative Propagation of Spilanthes Acmella (L.) Murr
Author name:
انسام زهير جاسم الحسني
Supervisor name:
بشرى محمد جابر علوش
General topic:
Biology
Specific topic:
Plant
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
Arabic
University location:
Baghdad
First pages:
24T2912 - p.pdf
Abstract:
وظفت تقانة زراعة الانسجة لاكثار نبات Spilanthes acmella (L.) Murr. كونه نباتا غير مستزرع في العراق ولاهميته الطبية والزراعية بوصفه نبات زينة فضلا عن استعمالاته الاخرى. عقمت البذور بمادة هايبوكلورات الصوديوم(NaOCl) وزرعت على وسط Murashige وSkoog (1962) (MS | The technique of plant tissue culture has been manipulated to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized by sodium hypochlorite (NaOCl) and cultuared on full strength Murashige and Skoog medium(1962) (MS). Nodal segments, apical shoots, and leaflets explants were taken from seedlings and cultured on (MS) medium enriched with different concentrations of Indol acetic acid (IAA), Naphthalene acetic acid (NAA) Benzyl adenine (BA), and 6 - furfurylaminopurine (Kin.). The study include the effect of node orientation vertically and horizontally on induction of shoots formation. The effect of the interaction between IAA and BA, IAA and Kin. on shoot multiplication was investigated. Rooting was also studied after inclusion of IBA and IAA to full and half - strength (MS) medium. At acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. DNA isolation was carried out from fresh and dry plantlet leaves as well as from seeds and Agarose Gel Electrophoresis (AGE) was done. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. The nodes cultured vertically exhibited relatively highest response as compared with apical shoots and leaflets culture. Supplying the culture medium with 1 mg/L. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding IAA at 0.1 and Kin. at 0.5, 1.0 or 1.5 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 14.20, 13.60 shoots/ explants with a mean length of 3.40, 2.60, 1.40 cm, respectively. Adding 0.1, 0.5, 1.0 mg/L IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots, respectively, with mean length of 3.25, 3.80, 3.80 cm, respectively. Plantlets were acclimatized successfully achieving 100% survival after four weeks when transferred to agricultural medium consisted of river sand and peat moss at 1 : 1 v/v ratio. This study showed the ability of in vitro micropropagation of Spilanthes acmella (L.) Murr. DNA isolation from drying leaves was butter than fresh leaves with the precense of liquid nitrogen.
