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دراسة وراثية جزيئية لبكتريا المكورات المعوية البرازية Enterococcus faecalis المعزولة من جذور الاسنان الملتهبة للانسان في بغداد == Molecular Genetics Study of Enterococcus Faecalis Isolated From Root Canal Infection of Human In Bagdad
Author name:
سوزان علي كاظم
Supervisor name:
عذراء حميد حسون
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Baghdad
First pages:
24T2922 - p.pdf
Abstract:
تم التحري عن نسبة وجود بكتريا المكورات المعوية البرازية Enterococcus faecalis في (100) عينة معزولة من اشخاص مصابين بالتهاب قناة جذر السن, اذ تم جمع (70) عينة من الاصابات الابتدائية لقناة الجذر، و(30) عينة من الاصابات الثانوية لقناة الجذر (اعادة العلاج) وم | To detection Enterococcus faecalis in (100) root canal sample were collected from primary and secondary root canal infection patient`s from all the ages (10 - 50) during the period of (August 2013) till (January 2014). Detection depending on cultural & microscobial characteristics of bacterial cell was done to find (45) 0f E. faecalis species & Biochemical tests & Serological diagnosis by Lancefield method done to find (24) isolates of this species & Diagnosis by Vitek2 was done to find (20) isolates of E. faecalis. When the Molecular genetics Diagnosis was done the result showed find (32) isolates belong to E. faecalis. The antibiotic sensitivity test was done by using (14) antibiotics, (5) isolates showed resistant against all antibiotics & the isolates showed multiresistant against for some antibiotics. All the isolates were resistant by (100 %) against (5) antibiotics. To detection of the isolates ability of production of protease enzyme, lipase enzyme, hemolysin enzyme & gelatinase enzyme.The results showed that (24 isolate) (75 %) were protease producer, & (8 isolates) (25 %) were lipase producer, & (16 isolate) (50 %) were hemolysin producer, & (5 isolates) (15.6 %) were gelatin producer. To detection the presence efa A gene of the isolates by used specific primer to this gene, and all isolates
