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دراسة بعض القياسات الكيموحياتية والمناعية في مرض الفصام واضطرابات الاكتئاب الشديد في مدينة النجف الاشرف == Study of Somebiochemical And Immunological Parameters In Schizophrenia And Major Depressive Disorders In Najaf Alashraf Province
Author name:
دعاء عبد الزهرة محمد علي الرماحي
Supervisor name:
حسين كاظم الحكيم
General topic:
Chemistry
Specific topic:
Chemistry
Degree:
Master
University:
University of Kufa - College Of Science - Chemistry Department
Language:
English
University location:
Najaf
First pages:
25T1430 - p.pdf
Abstract:
شملت هذه الدراسة (56) عينة لادرار وامصال دم المرضى المصابين باورام البروستات ومقارنتها مع (62) عينة من الاصحاء وكانت اعمارهم تتراوح (40 - 80) سنة.ولقد اظهرت الدراسة النتائج الاتية : 1 - تم قياس نشاط انزيم اللاكتيت ديهايدروجنيز (LDH) في ادرار وامصال دم ا | This study was performed on (56) patients with prostatic tumors , in addition (62) apparently healthy subjects with ages ranged (40 - 80) years were investigated as a control group.This study showed the following results : 1 - Total serum protein in prostatic tumor patients showed a significant decrease in comparison to healthy subjects. While urinary protein and creatinine showed a significant increase. 2 - Patients with prostatic tumors showed a highly significant increase (P< 0.05) in serum and urine Lactate dehydrogenase (LDH) activity compared to healthy subjects. 3 - A significant increased in activity of acid phosphatase (ACP) and prostatic acid phosphatae (PAP) was shown in serum of prostatic tumor patients compared to apparently healthy subjects.4 - The results revealed a positive correlation of age with (LDH) activity in patients urine and with (PAP) activity in patients serum in addition there was a positive correlation of (LDH) activity in patients urine with (PAP) activity in patients serum. 5 - LDH isoenzymes were isolated and partially purified from patients urine , by using gel filtration (Sephadex G - 150) and ion - exchange, chromatography (DEAE - Cellulose A - 50) with pH gradient.6 - Kinetic Studies of (LDH) isoenzyme were carried out , which included the effect of different concentrations of substrates (Sodium pyruvate and NADH) , pH and temperate. The Km values of isoenzym (LDH1) were 2.6 , 51.7 for sodium pyruvate and NADH respectively (by Lineweaver - Burk piot) and 2.57 , 50 (by Hans - Woolf plot) and the optimum pH was 7 , optimum temperature was 50 C0. 7 - The isoenzyme (LDH1) obeyed Arrhenius equation and its (Ea) and (Q10) constants were determined
