تاثير المستخلصات المائية والكحولية لنباتات ليمون البصرة والرمان والبلوط على البكتريا المسببة للاسهال == The Effect of Aqueous And Alcoholic Extracts of The Plants (Aurantifolia Varacidica, Punica Granatum, And Quercus Robur) On The Bacteria Causing Diarrhea
Author name:
نور جبار احمد علي
Supervisor name:
نجم عبد الله جمعة الزبيدي
General topic:
Biology
Specific topic:
Plant
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Diyala
First pages:
24T3010 - p.pdf
Abstract:
اجريت الدراسة في محافظة ديالى للمدة من 17 اب 2014 ولغاية 15 شباط 2015, اذ اخذت اربع عزلات جرثومية جاهزة بعد عزلها من حالات مرض الاسهال وهي Proteus mirabilis وKlebseilla pneumonia وEscherichia coli وSalmonella typhimurium0تناولت الدراسة تاثير مستخلصات نب | This study was conducted in Diyalaprovince during the period that lasted from 17 August, 2014 to 15 February 2015, four ready bacterial isolates were taken after isolating them from diarrhea disease cases and then they were diagnosed by the bactriology in the bacteriology lab/ Baqubah teaching hospital.The isolates were : Salmonella typhimurum, Escherichia coli, Klebseilla pnumonia, and Proteus mirabilis. The study investagated the effect of the plants extracts of Punicagranatum, Quercusrobur, and aurantifoliavaracidica Citrus on the bacterial isolates that caused diarrhea.The bacterial isolates showed a various sensitivity toward the plants extracts. The sensitivity of isolates against extracts were to alcoholic extract, hot aqueous extracts cold water extracts of all the plants used in the study The study showed that plant extract ofQuercusroburwas the more effect on the growth of the bacterial isolates Punicagranatum extractsand extract of Aurantifoliavaracidica respectively. The high inhibition zone concentration were at 100 and 80 mg /ml. The isolates that had moer sensitivity toward the plants extracts were : Klebseilla pnumonia, Salmonella typhimurum, Proteus mirabilis and Escherichia coli respectively. The study investigated with the qualitative and quantitative of active compounds found in the plants extracts used in the study. The result showed that the plant were rich of many secondary metabolic compounds like : (Taninat, clycosides, flavonoids, alkaloids, pheonols and saponins) Also, the present study showed the toxid effect of the aqueous and alcoholic extracts on the red blood cells. it showed also that there was cytotoxic of the cold and hot aqueous extract for all the plants used in the study. The result showed the resistant of the bacterial isolates against some antibiotics such as Ciprofloxacin, Cefotaxime, Amikacin, trimethoprim, Gentamicin and Nalidixic acid. The study showed that the bacterial isolates had a highest ratio of resistance against the antibiotics Amikacin, Trimethoprim, and Cefotaxime
