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فحص عوامل عنق الرحم باستخدام فحصي اختراق النطف واتصال النطف بمخاط عنق الرحم للازواج العقيمين == Examination The Cervical Factors By Using Sperm Penetration Test And Sperm Cervical Mucus Contact Test In Infertile Couples
Author name:
الاء عمران مطلك الحسناوي
Supervisor name:
فارس ناجي عبود الهادي | بشرى جابر الربيعي
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Babylon - College Of Science - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T3023 - p.pdf
Abstract:
The present study was conducted in Infertility unit / Babylon Hospital of Gynecology and Children in a period 21/1/2013 to 15/1/2014. The study involved communiqués tests for males and females complaining from unexplained infertility , in which seminal fluid analysis were normospermia. The study include 45 seminal fluid specimens used in connecting test (SCMCT) and penetrating test (SCMPT) with the specimens of cervical mucus of their wives. Cervical mucus aspirating in day 12 - 14 of menstrual cycle. The study aimed to evaluate the husband's sperm activity and concentration in vitro with cervical mucus. Seminal fluid analysis were achieved and then semen specimens used to performed two in vitro tests : SCMCT and SCMPT. sperm parameters ( sperm concentration and sperm motility grade a, b, c, and d) were evaluated in each mentioned test and compared the results with the values of sperm parameters in seminal fluid analysis (control). The statistic results showed 60% of the study specimens of SCMCT was negative and 40% positive, while in percentage of positive results was 33% and 67% negative in penetrating test. The results revealed a significant (p<0.001) decrease of sperm concentration and grade a and b sperm motility, a significant (p < 0.001) increase of sperm motility grade c and immotile sperm grade d in connecting test with cervical mucus compared to control. The results of penetrating test showed significant (P< 0.001) decrease of sperm concentration in the brim, medium and distal region of cervical decrease (p<0.001) of sperm concentration in both medium and distal region compared to brim region, and a significant (p < 0. 001) decrease in distal region compared to medium region ; A significant (P < 0.001) decrease of grade a + b motility percent in all three regions of cervical mucus area compared to control, while no significant differences (P > 0. 05) between brim, medium and distal region of cervical area ; Only grade C sperm motility in distal region showed a significant decrease (P < 0. 001) compared to control and both brim and medium regions. The results revealed significant increase of grade d (P<0.001) in brim and medium region (P < 0.01) compared to control, while there was a significant decrease (P< 0. 001) in distal region compared to control, brim and medium regions. The correlation (r) study of sperm concentration and sperm motility ( grade : a + b, c and d) with age and infertility period in SCMCT showed non significant (P > 0. 05) correlation, except the correlation between a + b grade sperm motility and age it was noticed a significant negative correlation (P < 0. 05) and positive correlation(p> 0.05) between grade c sperm motility and age. The study of linear correlation of sperm parameters in penetrating test showed a significant(P<0.05) negative correlation between grade a+b sperm motility and age in brim and medium region of cervical mucus area. Except this, there are no significant correlation (P>0.05) between sperm concentration and sperm motility for all grades (a + b, c and d) with age and infertility period in both brim and medium regions It was concluded that both test : SCMCT and SCMPT were necessary to diagnosis the causes of infertility when there were normal finding of both seminal fluid analysis and female criteria. mucus area compared to sperm concentration in control, also a significant
