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دراسة فعالية المشتت الحيوي السطحي المستخلص من بكتريا Bifidobacterium spp في تثبيط الغشاء الحيوي للمسببات المرضية المعزولة من مرضى القسطرة القلبية وتاثيره في عملية البلعمة == Study of The Effectiveness of Biosurfactant Extracted From Bacteria Bifidobacterium Spp. In The Inhibition of Biofilm of Pathogens Isolated From Cardiac Catheterization Patients And Its Effect In Phagocytosis
Author name:
بتول شاكر عبد المجلاوي
Supervisor name:
هيام عبد الرضا كريم العواد | علي رحيم حنظل الهامل
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Kerbala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Karbala
First pages:
24T2905 - p.pdf
Abstract:
This study was conducted in order to assess the effect of Biosurfactant extracted from bacteria Bifidobacterium spp in the inhibition of Biofilm produced by pathogenesis bacterial isolated from patients with during cardiac catheterization, This study getting 89 patients with cardiac catheterization unit in AL - Imam AL - Hussain Teaching Hospital from both genders of different ages ranged between (29 - 75) years, starting from (January 2014 and to the end of July 2014). The samples taken from atherosclerosis patients were cultured for all types of cardiac catheterization by three tests Swabs, catheter tip culture and blood culture sample before and after a catheter for the purpose of isolating pathogens that component of the biofilm of patients with atherosclerosis. The results of the current study showed that patients with diagnostic cardiac catheterization rate of 66 (74.16%) patients were given 15 (60%), 15 (60%), 14 (58.33%) and 15 (62.5%) bacterial growth, respectively. But patients with therapeutic cardiac catheterization of 15 (16.85%) patients were given 6 (24%), 6 (24%), 6 (25%) and 6 (25%) bacterial growth respectively, and diagnostic and therapeutic cardiac catheterization for together 8 (8.99 %) patients were given 4 (16%) 0.4 (16%), 4 (14.67%) and 3 (12.5%), bacterial growth, respectively. The results showed sensitivity and specificity values for swabs test 96%, 100%. while the catheter tip culture was 96% and 100% respectively, and blood culture was 100 and 98%, respectively, it became clear from the results after the sample cultivations on the enrichment and differential media that 89 patients (sample joint) gave 25 (28.08%) positive sample for bacterial cultivation, and two of them showed two types of bacteria to become 27 isolated bacterial, isolates were diagnosed 11 (70.74%) gram positive, 15 (55.56%) gram negative , one isolate only of the yeasts 1 (3.70%), and 64 (71.91%) did not give any growth. Ability of pathological isolates were tested for the production of Biofilm by using three different methods, tube method TM, Congo Red Agar CRA, microtiter plate M TP were given 25 (92.6%), 16 (58.26%) and 26 (96.29%) the isolate of producing biofilm by different quantities respectively. The results of the investigation of formation biofilm test showed that MTP has 100% sensitivity and 96% specificity, the TM sensitivity was 96% and 100% specificity and CRA was sensitivity of 61.5% and 100% specificity. One hundred and fifteen samples were collected from of local and imported dairy products 45 (39.13%), breast milk 32 (27.83%) and cows milk, 38 (33.04%) were obtained at 22 (19.13%) positive sample of Bifidobacterium bacteria after morphological, microscopic and biochemical diagnosis tests and comparing these isolates with standard isolates. Oil Spreding Techingue and blood Hemolysis was performed to investigate the capability Bifidobacterium on the production biosurfactant, the efficiency antithesis test was studied of liquid bacterial farm for Bifidobacterium bacteria against bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, E coli, Klebsiella pneumoniae , and Serratia marcescens was diameters of inhibition zones (24.25, 21, 24, 22, 23) mm respectively. The inhibitory effectiveness was Studied for filtrate against bacteria Staphylococcus epidermidis, S.aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, P.aeruginosa, Enterobacter Cloacae and Serratia mercescens, where the E.coli bacteria was more effective and P.aeruginosa less effective by the bacterial filtrate. The study included the effect of three concentrations of biosurfactant against bacteria test group, and the diameters of inhibition rates of inhibition areas to concentrations 50% as follows (33.34, 30.34, 32.30, 33.32) mm respectively. The diameters rates of zones of inhibition for the concentration of 25% was as follows (30.32, 27.32, 31.28, 31.5, 29) mm respectively, and zones inhibition rates of concentration of 12.5% as follows (28.29, 24.30, 26.26, 28.26) mm respectively, statistically significant differences at the significance level of P <0.01inhibition in rates depending on the type of user concentration and statistically significant differences between the types of bacteria in their affected by biosurfactant. also determine the values of (MIC) Minimum Inhibitory Concentraction, (SubMIC) Sub Minimum Inhibitory Concentraction and (MBC) Minimum Bactericidal Concentraction, the value of MIC 6.25 mg / ml, Sub MIC 3.12 mg / ml and MBC 12.5 mg / ml of the bacteria E.coli, K. pneumoniae, Enterobacter cloacae, and the value of MIC amounted to 12.5 mg / ml, Sub MIC 6.25 mg / ml and MBC 25 mg / ml of bacteria S.aureus, S.epidermidis, P. mirabilis, P. aeruginosa and Serratia mercescens. The susceptibility of biosurfactant was studied to inhibit biofilm bacteria by MTP method The results showed a significant decrease in the formation of biofilm after the addition of biosurfactant compared with the biofilm before adding biosurfactant where the decreased rate reached 96%. Effect of biosurfactant test was performed on the efficiency of phagocytosis in vitro the percentage of phagocytosis reached 40.9% of the control group 64.8% of the experimental group. Concluded that the biosurfactant has a clear inhibitory efficacy against pathogens isolated from patients with cardiac catheterization and inhibition of biofilm for pathogens as well as raising the efficiency of the process of phagocytosis in vitro
