Share
انتشار بكتريا Escherichia coli في المرضى في المرضى المصابين بالتهاب المرارة الحصوي في محافظة الديوانية == Dissemination of Escherichia Coli In Patients With Cholecystitis Calculus In Al - Diwanyia Governorate
Author name:
احمد رزاق واجد الداوودي
Supervisor name:
سيوف خومان علوان الرماحي
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Al-Qadisiyah - College Of Science - Department Of Biology
Language:
Arabic
University location:
Qadisiyah
First pages:
24T3001 - p.pdf
Abstract:
The study aims to identify Escherichia coli bacteria, which causes calculus cholecystitis of molecular perspective. The study samples were gathered from 110 patients who undergone cholecystectomy in Diwaniya general hospital for the period October 2013 to April 2014. The sample included bile sap, gall bladder tissues and stone if available. The samples were cultured to identify their culture specifications.The results showed that chronic cholecystitis is the most common one, which has 55.5% then acute cholecystitis by 44.5%. The study results showed that age and sex have an influence on cholecystitis. The highest percentage of infection is the ages 36 - 45 for the two sexes. If the cases distributed according to the patient sex, there is a difference in cholecystitis between male and female, where females infections are 88.2% and males are 11.8%. The total isolated bacteria were 125 bacterial isolates of 52% of gall bladder tissues, 26.4% of bile and 21.6% of the stone. Escherichia coli had 34.4% of the total samples of 51.1%, 36.6% and 29.6% respectively of the above mentioned samples.As far as susceptibility for all Escherichia coli isolates towards 15 types of antibiotics by using disc diffusion for Kerby - Bauer method, bacteria had high susceptibility against ? - lactamases antibiotics where they had susceptibility against Ampicillin+ Clavulanic acid by 38.7% and 79% for Ampicillin. The least susceptibility was for Amoxicillin by 69.7%. Bacteria had no resistance for Imipenem where their susceptibility was 100%. Bacteria had weak susceptibility against Aztreonam by 13.9%. While with cephalosporins, susceptibility for Ceftazidime by 27.9% and for Cefotaxime by 20.9%. With Aminoglycosides, resistance to Gentamicin antibiotics was 34.8% and 30.2% for Amikacin. The results showed that resistance to Quinolones was 16.2 for Nalidixic acid while for fluoroquinolones resistance was 25.5% for Ciprofloxacin and 18.6% for Norfloxacin. Isolates ability to produce CTX - M, SHV, TEM and AMPC extended spectrum ? - lactamases enzymes was tested by investigating the existence of blaTem, blaAMC, blaCTX - M and blaSHV genes in these isolates through Polymerase chain reaction (PCR). There were 23/26 isolates (88.4%) showed their ability to contain blaAmpC gene, and 7/26 isolates (26.9%) contained blaSHV gene. There are 18/26 isolates(69.2%) contained blaTEM gene while 20/26 isolates (76.9%) contained blaCTX - M.Through using Quantitative Real Time - Polymerase chain Reaction (qRT - PCR), gene expression measurement for blaCTX - M, blaTEM and blaAmpC was measured by relative numbers. The results showed that gene expression measurement for ? - lactamases enzymes type AmpC produce by Escherichia coli was the highest if compared with ? - lactamases enzymes type TEM and CTX - M with different periods of incubation, which is T4h for 4 hours, T24h for 24 hours and T48h hours of incubation respectively. It is noticed that genes responsible for producing ? - lactamase enzymes type TEM, CTX - M and AmpC are changed during different periods of incubation through using Real Time - Polymerase chain Reaction (RT - PCR).
