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التغيرات النسيجية في المشايم والاجنة نتيجة الاصابة بداء المقوسات في النساء المفحوصات بقياس الIgG == Placental And Fetal Tissue Structural Changes Resulting From Congenital Toxoplasmosis In Women Diagnosed By IgG
Author name:
حلا ياسين كاظم
Supervisor name:
طالب جواد كاظم | نغم ياسين البياتي
General topic:
Biology
Specific topic:
Zoology - Histology
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
English
University location:
Diyala
First pages:
24T2888 - p.pdf
Abstract:
طفيلي المقوس الكونديToxoplasma gondii هو طفيلي ابتدائي اجباري داخل الخلايا ذات النواة. له القابلية على اصابة جميع حيوانات الدم الحار ويعد هذا طفيلي ذو اهمية صحية نظرا لما له من تاثيرات مرضية. لقد اجريت عدة دراسات بينت التغيرات النسيجية التي اثرت على انسجة | Toxoplasma gondii is an a zoonotic, obligate intracellular protozoan parasite that has the capacity to infect all warm - blooded animals. Many studies done to present the histological changes of the placental and fetal tissue and organs that resulted by Toxoplasmosis infection of pregnant women and about identification of infected fetal organs, but a lot of these studies were done experimentally on laboratory animals, accordingly the present study aims to study the structural changes of the placenta and fetal tissue and to identify the fetal organs in which the organism localized that taken from aborted fetus and embryos from pregnant woman that diagnosed as infected with Toxoplasma gondii. The present study was carried out during the period December 2012 to March 2013. Eighty women who had abortion chosen randomly from that which were revised gynecology theater in hospitals in Baquba city. Their age were ranged between 16 - 45 years, (10) fetuses and (33) samples of them non - infected as control group (c) and (37) samples were infected with Toxoplasmosis (I). The study included serological examination for mothers and Macropathlogical (Gross) , histopatholgical examination and Immunohistochimcal (IHC) stain of the placenta and fetal organs. Toxoplasmosis diagnosed serologically by ELISA (Enzyme Linked immune Sorrbant Assay) test. Immuno - histological techniques are used to detect the antigen and determination of their morphological localization in fetal and placental tissue. Formalin fixed paraffin embedded tissues (placenta and specimens of organs from aborted fetus) used to determined the antigen (Toxoplasma gondii) to detect the histological changes in these tissues. The ordinary histopathlogical changes of the tissue taken from aborted fetus showed different infiltration inflammatory cell and necrosis changes in brain, liver, lung, upper and lower limbs, kidney and spleen that may be due to infection of the parasite and not all infected organs localized the parasite (Tachyzoites). The results of the study showed that (46.26%) of the randomly selected mothers among that revised to the delivery theaters and had abortion. The fetal tissue and organs of the age (8 - 24) weeks gave positive (+) result for IHC in fetuses(brain and lung) and tachyzoites was detected in that organs, but fetus tissue of the age (4 - 8) weeks was negative for that test. Macropathlogical study of the placenta showed that the weight of the infected placenta was less than the normal ranges. and calcification was not significant in the infected placenta(45.7%) compared with non infected samples (4.28%). Infarction observed high significant in infected group was 25 (35.71%) compared with control group (2.85%) (p=0.001).Necrotic foci also seen as (40%) in infected group compared with control group (14.28%), differences between the two groups was statistically significant (p=0.001). Also congestion observed high significant (p=0.001) and the congestion was seen in (45.71) in infected group and (5.71%) in control group. Histopathlogical examination for placenta showed hemorrhage, necrosis, fibroniod necrosis and degeneration in trophoblastic cell, in both first and second trimester of pregnancy. Villous vascularity observed decreased as (32.85%) in infected group compared with control group (2.85%). Fibronoid necrosis in villi showed high percentage in villi which have > 3 that observed in infected group as (72.9%) compared with control (12.12%). An increased in villous stromal fibrosis was seen in (70.27%) placenta in infected group compared with control group which was (24.24%)
