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قياس الفعالية الانزيمية وتحديد الطفرات الجينية لانزيم الثايوبيورين الناقل لمجموع المثيل في عينة من الاطفال العراقيين المصابين بسرطان الدم اللمفاوي الحاد == Thiopurine S - Methyltransferase Phenotype and Genotype in a Sample of Iraqi Children with Acute Lymphoblastic Leukemia

Author name: نوار سمير محمد
Supervisor name: منال كمال رشيد | حسنين حبيب غالي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Background : The most common childhood cancer is acute lymphoblastic leukemia which is only treated with chemotherapy alone.All modern protocols of acute lymphoblastic leukemia treatment used thiopurine drugs as an essential anti - cancer drug which used for a long period of time. The 6 - Mercaptopurine is an anti - cancer drug widely used for treating acute lymphoblastic leukemia patients. The patients with low Thiopurine S - Methyltransferase enzyme activity is with an increased risk of developing drug toxicity and consequently unsuccessful acute lymphoblastic leukemia outcome and even death.Thiopurine S - Methyltransferase is one of the main enzymes involved in 6 - mercaptopurine metabolism, and the low activity of this enzyme is strongly correlated to the Thiopurine S - Methyltransferase genetic polymorphism.Aim : Find out the three most common Thiopurine S - Methyltransferase enzyme polymorphism TPMT*3A, TPMT*3B and TPMT*3C in Iraqi pediatric patients with acute lymphoblastic leukemia, and its frequencies. Analyses of Thiopurine S - Methyltransferase activity in the serum of those patients, and compare the results with other population. Methods : This is a cross - sectional study included eighty - one (81) Iraqi pediatric patients with acute lymphoblastic leukemia during the maintenance phase of their UKALL protocol treatment, receiving 6 - Mercaptopurine drug with age range from 1.83 (1year and 10 months) to 16.25 (16 years and 3 months). Thiopurine S - Methyltransferase activity was measured in the patients’ serum by Enzyme - Linked Immunosorbent Assay technique and three of Thiopurine SMethyltransferase genetic polymorphisms were detected by allelespecific multiplex - PCR analysis after DNA extraction from the whole blood. Liver Function Tests were measured by calorimetric method; Alanine Aminotransferase, Aspartate Aminotransferase, Alkaline Phosphatase and Total Serum Bilirubin in addition to Complete BloodCount measured by automated hematology system. Results : There was significant difference in the mean of Thiopurine S - Methyltransferase activity between pediatric patients carrying the wild - type allele TPMT*1 (n=49), with allele frequencies of 60.4% and pediatric patients (n=32) carrying the mutant alleles (TPMT*3A or TPMT*3C) with allele frequencies of 81.2% and 18.7% respectively. The TPMT*3B allele was not detected in this group. The P - value was highly significant (P<0.000**).Conclusion : This study is the first to analyze Thiopurine SMethyltransferase mutant gene frequency in a sample of the Iraqi population, and it revealed the presence of TPMT*3A and TPMT*3C genetic polymorphism but not a TPMT*3B mutant allele. Thiopurine S - Methyltransferase activity was low in the patients with mutant gene as compared with the wild - type allele patients. Finally, genotype and phenotype of Thiopurine S - Methyltransferase enzyme is an essential predictor to reduce the cytotoxic effects of the anticancer drug and successful acute lymphoblastic leukemia treatment.

تركيزات البلازما والاداة المساعدة للتشخيص لعامل النمو البطاني الوعائي (VEGF) ، مصفوفة ميتالوبروتيناز - 2 (MMP - 2) ، مثبطات الانسجة لمركب الميتالوبروتيناز - 2 (TIMP - 2) والمستعمرة الضخمة (M - CSF) في النساء اللواتي يعانين من ورم الثدي : دراسة مقارنة مع م == Plasma Concentrations and Diagnostic Utility of Vascular Endothelial Growth Factor (VEGF), Matrix Metalloproteinase - 2 (MMP - 2), Tissue Inhibitors of Metalloproteinase - 2 (TIMP - 2), and Macrophage Colony Stimulating Factor (M - CSF) in Women with Brea

Author name: اسيل نبيل كامل
Supervisor name: باسل عويد محمد صالح | كفاح حمدان
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: سرطان الثدي هو الورم الخبيث الاكثر شيوعا في النساءويعتبرالسبب الرئيسي الثاني لوفاة مرضى السرطان في العالم. تعتبر الوقاية والكشف المبكر هوالطريقة الاكثر فعالية لمكافحة السرطان. خلال العقد الماضي ، جذبت الكثير من علامات البيوكيميائية لسرطان الثدي انتباه العديد من الباحثين. هذه العلامات يمكن استخدامها في مراقبة العلاج ، والتنبؤ بدرجة اصابة الانسجة ، وسلوك الورم ، ومدى انتشاره ، واحتمالية اصابةالعقد الليمفاوية. من بين هذه الواصمات الحيوية ، عامل نمو بطانة الاوعية الدموية (VEGF) ، خلايا البلعوم المحفزة للحفظ (M - CSF)، ومصفوفة الميتالوبروتنيز MMP - 2))، ومثبطات الانسجة لمركب الميتالوبروتينيز(TIMP - 2). تشير الدلائل المتزايدة الى ان قياس VEGF هو افضل مرشح لتشخيص سرطان الثدي (افضل من مستضد السرطانCA 15 - 3 ) خاصة في المرحلتين الاولى والثانية وكذلك في التمايز بين الورم الخبيث وورم الثدي الحميد. اظهرت الدراسات ان قياس VEGF لا سيما مع CA 15 - 3 ، اعلى فائدة وقوة تشخيصية في الكشف عن سرطان الثدي.وفي الاونة الاخيرة ، تم التوصل في البحوث الحديثة الى ان TIMP - 2 قد يكون ذو فائدة في التشخيص المبكر لسرطان الثدي في مراحله الاولى زتهدف هذه الدراسة الى : 1) قياس تركيزات البلازما لبعض المرقمات الحيوية الجديدة بما في ذلك : عامل نمو بطانة الاوعية الدموية (VEGF) ، ومصفوفة الميتالوبروتينيز ((MMP - 2 ، ومثبط الانسجة لمركب الميتالوبروتينيز ((TIMP - 2 ، وعامل تحفيز مستعمرة البلعم (M - CSF) بالاضافة الى مثبط السرطان 15 - 3 ( CA15 - 3) في النساء المرضى الذين يعانون من ورم الثدي بالمقارنة مع النساء الاصحاء.2) التحقيق في الاداة التشخيصية لكل من المرقم الحيوي المقاس (وبالاقتران مع بعضها البعض) في الكشف عن ورم الثدي و(3) مقارنة النتائج مع معلمات الهستوباثولوجي ( العمر, الجنس , الحالة الزوجية , سن الياس حجم الورم , مرحلة الورم السرطاني , مستقبل الاستروجين , مستقبل الروجيستيرون ومستقبلات 2 Her)المواد وطرق العمل : اشتملت الدراسة على 88 امراة عراقية تم تقسيمهم الى ثلاثة مجاميع : 38 امراة مصابة بسرطان الثدي (Group I, BC) ، 25 امراة مصابات بورم الثدي الحميد الغدي الليفي Fibroadenoma (FA) (Group II, FA benign ) اللواتي تم تشخيصهن من قبل مجموعة اخصائي الاورام و25 امراة سليمة (كمجموعة ضابطة). تم تشخيص ورم الثدي من قبل مجموعة من الاخصائيين في الاورام. حيث تم التشخيص بناء فحوصات تجرى على عينة تؤخذ من خزعة نسيجية من ورم الثدي او بعد استئصال الثدي. تم تنفيذ اجراءات التدريج العلاجي على اساس فحوصات الجسم والدم ، والتصوير الشعاعي للثدي ، وفحص الثدي بالموجات فوق الصوتية ، والاشعة السينية للصدر حسب توفرها وضرورتها. تم اجراء تصنيف الاورام وتدريجها اعتمادا على معايير الاتحاد الدولي لمكافحة سرطان الاورام - (UICC - TNM) واللجنة الامريكية المشتركة لمرحلة مكافحة السرطان. تم تقسيم النساء المصابات بسرطان الثدي الى مجموعات فرعية على اساس مراحل السرطان ؛ المجموعة (ا) : تشمل 12 امراة في المرحلة الاولى (T1N0M0) ، حيث حجم الورم <2 سم ، المجموعة ب : 14 امراة في المرحلة الثانية (T2N0M0 ، T2N1M0 ، T3N0M0) ، حجم الورم (2 - 5) سم ومجموعة C : تشمل 12 امراة في المرحلة الثالثة (T2N2M0 ، T3N1M0 ، T3N2M0 وT4N2M0) ، كما كان لديهم حجم الورم > 5 سم. في هذه الدراسة تم استبعاد النساء المصابات بسرطان الثدي في المرحلة المتقدمة (المرحلة الرابعة) ، والنساء اللواتي يعانين من المرض سابقا او اللواتي يعانين من انواع اخرى من السرطانات؛ السرطانات التناسلية الانثوية (سرطان المبيض ، سرطان عنق الرحم ، سرطان الرحم) ، اورام الكلى ، القولون والمستقيم ، البنكرياس ، الرئة والراس والعنق. ايضا ، تم استبعاد المدخنين والنساء اللواتي يتناولن المشروبات الكحولية من هذه الدراسة. تم سحب عينة الدم من كل امراة لقياس VEGF ، M - CSF ،TIMP - 2,MMP - 2 و3 CA15 - . ايضا تم قياس مصل 25 هيدروكسي فيتامين D (25OHD) ومعلمات الدهون بما في ذلك الكولسترول الكلي (TC) ، والدهون الثلاثية (TG) ، والكوليسترول الدهني عالي الكثافة (HDL - C) ، والكولسترول الدهني منخفض الكثافة (LDL - C) فقط في المجموعة الاولى والمجموعة الثانية | Breast cancer (BC) is the most common malignancy in women and the second leading cause of their death from cancer in the world. The most effective way to combat cancer is its prevention and early detection. During the last decade, biochemical markers of breast cancer have attracted the attention of many researchers. Among these biomarkers, the interest points to Vascular Endothelial Growth Factor (VEGF), Macrophage Colony Stimulating Factor (M - CSF), Matrix Metalloproteinase - 2 (MMP - 2), Tissue Inhibitors of Metalloproteinase - 2 (TIMP - 2). Growing evidence indicates that measurement of VEGF maybe the best candidate for BC diagnosis (better than CA 15 - 3) especially in stages I and II as well as in the differentiation between BC and benign breast tumor. More recently it has been reported that TIMP - 2 maybe useful in early diagnosis of BC and differentiation of breast cancer stages. The aim of this study are to : (1) Measure the plasma concentrations of some of new biomarkers including : Vascular Endothelial Growth Factor (VEGF), Matrix Metalloproteinase - 2 (MMP - 2), Tissue Inhibitor of Metalloproteinase - 2 (TIMP - 2) and Macrophage - Colony Stimulating Factor (M - CSF) in addition to CA 15 - 3 in women patients with breast tumor in compare son with healthy control women, (2) Investigate the diagnostic utility of each of the measured biomarker (and in combination with each other) in detection of breast tumor and (3) Correlate the findings with the clinicopathological parameters (age, menopause, marital status, stages, tumor size, Estrogen Receptor (ER), Progesterone Receptor (PR) and Her - 2/neu receptor) in those patients.Subjects and Methods : Eighty - eight Iraqi women were enrolled in the study; 38 women with primary breast cancer (BC, Group I), 25 women with Fibroadenoma (FA) benign breast tumor (FA, Group II) and 25 apparently healthy women (served as control group). The diagnosis of breast tumor was achieved by the Oncology group. Histopathology investigations were performed by Consultant Histopathologic based on tissue biopsy of mammary tumor or after mastectomy. Tumor classification and staging were performed depending on criteria of International Union against Cancer Tumor - Node - Metastasis (UICC - TNM) classification and the American Joint Committee on Cancer Staging. Accordingly, women with breast cancer were classified into subgroups based on the stage of their BC; Group A : 12 women with stage I, Group B : 14 women with stage II, and Group C : 12 women with stage III. Exclusion criterion includes those women with breast cancer of advanced stage (stage IV), a previous history of multiple type of cancers; female reproductive tract cancers (ovarian, cervical and endometrial cancers), renal, colorectal, pancreatic, lung, head and neck tumors. Blood sample was aspirated from each woman for the measurement of plasma VEGF, M - CSF, MMP - 2, TIMP - 2 and serum CA15 - 3 by using enzyme linked immunosorbent assay (ELISA). Also, serum vitamin D3 was measured in group I only using ELISA and serum lipid profile parameters were measured in group I and group II. Results : The mean (±SD) values of plasma levels of VEGF (p<0.001), MMP - 2 (p< 0.001), TIMP - 2 (p < 0.001), M - CSF (p < 0.05), and CA 15 - 3 (p<0.001) of group I was significantly higher compared to that of group II and controls. However, the mean value of plasma MMP - 2 was the only measured biochemical marker which was significantly increased in group II compared to controls (p<0.001). Regarding the BC stages, the mean values of all the measured biochemical markers were significantly higher in stage III than in stage I and stage II (p<0.001). Interestingly, the mean (± SD) value of plasma TIMP - 2 levels was the only biochemical marker of the measured ones which was significantly increased in stage II than in stage I (p=0.001). The cut - point value of plasma VEGF (>55.73 pg/ml) or TIMP - 2 (>102 ng/ml) (for both, AUC=1.0) was the excellent test in differentiating women with stage I of BC from healthy women. While that of VEGF (>55.73 pg/ml), TIMP - 2 (>102 pg/ml) or CA15 - 3 (>10.82 u/ml) (for all, AUC=1.0) was considered as the best test in discriminating the BC stage II from healthy women. While, the cut - point value of plasma TIMP - 2 (>142 ng/ml) which has the highest (AUC=1.0) considered as the excellent test in differentiating BC stage I from women with fibroadenoma benign tumor (FA). While that of VEGF (>88.67 pg/ml) or TIMP - 2 (>254 ng/ml) which has the highest (for each, AUC=0.991) was considered as the best test in discriminating the BC stage II from FA. In addition, the plasma measurement of VEGF has the superior diagnostic utility in differentiation of FA women and healthy control women (p<0.001, AUC=0.998).Conclusion : Measurement of plasma VEGF (cutoff >55.22pg/ml) or TIMP - 2 (cutoff >102 ng/ml) is the best biochemical marker in diagnosis of stage I or stage II BC and differentiates them from healthy women; they have more diagnostic utility than CA 15 - 3. Also, measurement of TIMP (cutoff > 142 ng/ml) has the excellent diagnostic utility in diagnosis and differentiation of stage I BC from benign tumor fibroadenoma (FA). Moreover, TIMP - 2 (cutoff > 254 ng/ml) or VEGF (cutoff > 88.67 pg/ml) has the superior diagnostic utility over that of CA 15 - 3 in diagnosis and differentiation of stage II BC from FA. Plasma measurement of TIMP - 2 was the best biochemical marker in studying early progression of BC; it can differentiate stage II from stage I BC. Furthermore, plasma measurement of VEGF was found to be the excellent parameter in differentiation between women with FA from healthy ones.

تفاعل انزيم البلمرة المتسلسل النسخي العكسي ذي الوقت الواقعي كاداة تشخيصية جزيئية في التحري عن الجينات المدمجة الشائعة في عينة من الاطفال العراقيين المصابين بابيضاض الدم اللمفاوي الحاد == Real time reverse transcriptase polymerase chain reaction as diagnostic molecular tool in screening of common fusion genes in sample of Iraqi children with acute lymphoblastic leukemia

Author name: اريج عماد كاظم
Supervisor name: بان عباس عبد المجيد | سلمى عباس الحداد
General topic: Medicine
Specific topic: Diseases - Blood
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: ابيضاض الدم الليمفاوي الحاد عند الاطفال هو مرض متنوع له اصناف وراثية متعددة تستجيب بصورة مختلفة للعلاج الكيميائي. توجد الكثير من التغايرات الكروموسومية المؤدية الى انتاج جينات مدمجة مصاحبة لحدوث هذا المرض.اهداف الدراسة : لدراسة تعبير الجينات المدمجة الشائعة في ابيضاض الدم اللمفاوي الحاد عند الاطفال باستخدام تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي وربط نتائج هذه الدراسة مع مختلف المؤشرات السريرية والمختبرية ثم تقييم هذا التعبير بعد العلاج الاولي.المرضى, المواد وطرق العمل : اجريت هذه الدراسة المستقبلية للحالات والشواهد باستخدام تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي لدراسة تعبير الجينات المدمجة MLL - AF4 ,BCR - ABL1 ,E2A - PBX1 ,TEL - AML1 وSIL - TAL1 في ناضح نقي العظم ل 48 مريض بابيضاض الدم اللمفاوي الحاد قبل العلاج مع 46 شخوص ضابطة, تم جمعها من مستشفى حماية الطفل التعليمي / دائرة مدينة الطب للفترة من 1 تموز 2013 الى 31 حزيران 2014. استعمل جين GAPDH كجين مراقب داخلي لدراسة تعبير الجينات المذكورة بعد العلاج.النتائج : من اصل 48 مريض بابيضاض الدم اللمفاوي الحاد, كان هناك 21 ذكرا" و27 انثى مع نسبة ذكور الى الاناث 0.78 : 1 . تراوحت اعمارهم بين 2 شهر الى 13 عام مع متوسط عمر 5 اعوام . تراوحت اعمار 26 مريضا" بين 1 - 5 سنوات. اظهر الفحص الجزيئي الكشف عن نسخ الجينات المدمجة TEL - AML1، E2A - PBX1 وp190 1BCR - ABL في 20.8%, 16.7% و2.1% من المرضى، على التوالي، بينما لم يتم الكشف عن نسخ p210 1BCR - ABL ، MLL - AF4 وSIL - TAL1. بالاضافة الى ذلك، اظهر احد المرضى تعبير نسخي لكل من TEL - AML1 وE2A - PBX1 في الوقت نفسه. وجدت التعابير الجينية بعد بدء العلاج في 2 مرضى من اصل 12 مريض.الاستنتاجات : ان تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي طريقة جزيئية دقيقة وقابلة للتطبيق في تصنيف ابيضاض الدم اللمفاوي الحاد في الاطفال. ان نتائج التصنيف الجزيئي للاطفال العراقيين المصابين بابيضاض الدم اللمفاوي الحاد مشابهة نوعا" ما للتقارير العالمية جاعلة TEL - AML1 الجين المدمج الاكثر انتشارا". | Pediatric acute lymphoblastic leukemia is a heterogeneous disease with various genetic subtypes that respond differently to treatment. Many chromosomal aberrations with resultant fusion genes are known to be associated with the disease.Objectives : To study the expression of common fusion genes in pediatric acute lymphoblastic leukemia using a quantitative real time polymerase chain reaction technique, correlate results with different clinical and laboratory findings and evaluate these expressions after initial treatment.Subjects, materials and methods : A case - control prospective study was conducted using qPCR technique to study the expression of TEL - AML1, E2A - PBX1, BCR - ABL1, MLL - AF4 and SIL - TAL1 fusion genes in bone marrow aspirates of 48 untreated acute lymphoblastic leukemia pediatric patients and 46 control subjects, were recruited at the Children Welfare teaching hospital / Medical City directorate for the period from 1st of July 2013 to 31st of June 2014. Post - induction transcripts’ evaluation for 12 ALL patients was done by comparative quantification method using GAPDH as a reference gene.Results : Out of 48 acute lymphoblastic leukemia patients, 21 were males and 27 were females with a male to female ratio of 0.78 : 1. Age ranged from (2 months to 13 years) with 26 patients aged between 1 - 5 years whereas the median age was 5 years. The mean age was 5.9 years + standard error of 0.51 years. Molecular screening demonstrated detection of TEL - AML1, E2A - PBX1 and BCR - ABL1 p190 transcripts in 20.8%, 16.7% and 2.1% of patients, respectively. BCR - ABL1 p210, MLL - AF4 and SIL - TAL1 transcripts were not expressed. One patient expressed both TEL - AML1 and E2A - PBX1 transcripts. Post induction transcripts were detected in 2 out of 12 patients.Conclusions : Real time PCR technique is an applicable molecular method in classifying and predicting prognosis in pediatric ALL. The molecular classification of Iraqi children with ALL is mostly similar to reports worldwide making TEL - AML1 fusion gene the most prevalent type

الاهمية السريرية لدراسة كهروفسلجـة القلب لدى مرضى التسارع الاذيني الانتيابي حول العقدة الاذينية البطينية == CLINICAL IMPORTANCE OF CARDIAC ELECTROPHYSIOLOGICAL STUDY IN PATEINTS WITH ATRIOVENTRICULAR NODAL REENTRANT TACHYCARDIA

Author name: امـين عبد الحسن مانع العـلواني
General topic: Medicine
Specific topic: Diseases - Heart
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: تعتبردراسة كهروفسلجة القلب من الفحوصات القسطارية الضرورية لدى المرضى المصابيين بالتسارع الاذيني الانتيابي والذي يحدث بسسب ظاهرة عودة الدخول الكهربائي حول العقده الاذينية البطينية. تهدف هذه الدراسة الى معرفة الاهمية السريرية لكهروفسلجة القلب في تقييم مرضى التسارع الاذيني الانتيابي حول العقده الاذينيه . لقد خضعوا جميع المرضى الى اجراء قسطرة كهربائية القلب وخلال هذه العملية تتم دراسة كهروفسلجة القلب حيث تم مراقبة التوصيل الكهربائي بين حزمة هز والبطينين وتثبيت سرعة التيار الكهربائي بين الاذين والبطين اثناء التسارع الانتيابي. وشملت هذه الدراسةتسعة وثلاثون مريض من كلا الجنسين وباعمار مختلفة. ومن خلال دراسة كهروفسلجة القلب لديهم تم تقسيمهم الى مجموعتيين المجموعه النموذجية الاكثر انتشارا حسب قياسات المسافة بين البطين والاذين اثناء التسارع الاذيني الانتيابي والتي تكون اقل من 60 مل/ثانيه. وكان عددهم ثلاثة وثلاثون مريض. والمجوعة الثانية وهي اللانموذجية والاقل شيوعا وكان عددهم ستة مرضى. والمسافة بين البطين والاذين اثناء التسارع الانتيابي اكثر من 60 مل/الثانيه. اظهرت النتائج الى ان اكثر من نصف المرضى تكون العقده الاذينيه الحبيبه ذات فيزيولجية ثنائية الذراع (البطيئ والسريع) 62% وهو الغالب في العادة، ولكن من خلال هذه الدراسة وجدت نسبة تكاد تكون عالية 38% نسبيا بين هولاء المرضى حيث كانت العقدة الاذينية البطينية ثلاثية او رباعية الذراع حيث يشترك اكثر من ذراع بطيئ في التسارع الانتيابي. بعد الاعتماد على نتائج الدراسة وتحديد نوع التسارع الاذيني الانتيابي يتم كوي الحزمه الكهربائية في الذراع البطيئ بعد تحديد مكان الذراع بالاعتماد على التشريح والموقع اثناء الدراسة. مع الاخذ بنظر الاعتبار ان جميع المرضى تم كوي الذراع لهم وبشكل ناجح ولكن القسم الاكبر 59% تم قطع الذراع بشكل تام حيث لاثوجد اي اشارات لكهروفسلجة القلب بعد الكوي والقسم الاخر تم كوي الذراع بشكل غير كامل او تغير في التركيب التشريحي للذراع وكانوا 41% من المرضى بالاعتماد على وجود بعض المواصفات من البداية من دون رجوع التسارع الاذيني الانتيابي بعد الكوي خلال تحفيز القلب بعد الكوي. وتم الاستنتاج من خلال الدراسة ان النساء يعانون بنسبة اكبر من تسارع فوق الاذيني المنتظم مقارنة بالرجال. مع وجود نسبة ليست بالقليلة والتي تصل الى ثمانية وثلاثون بالمئة من المرضى تكون فيها فزيولوجية العقده الاذينيه ثلاثية او رباعية الذراع مقارنة بالفيزيولوجية ثنائية الذراع وهو الشائع بالعادة. | AV nodal reentrant tachycardia (AVNRT) considered as the most common regular supraventricular arrhythmia in humans. It originates from a location within the heart above the bundle of His. It is more common in women than men. It represents an important cause of palpitations and to lesser extent, dizzy spells and syncope. An invasive electrophysiological study is a helpful procedure in the classification of AVNRT into common (typical) and uncommon (atypical) types. This study aims to : (1) Assess the role of electrophysiological study in the classification of AVNRT. (2) Explore the presence of multiple pathways in respect to the usual dual AV nodal pathways. And (3) Used EP study as predictor for successful ablation of the slow pathway. This study was conducted on Thirty nine (39) patients of either sex with paroxysmal supraventricular tachycardia corresponding to an AVNRT referred for catheter ablation. Each subject was submitted for history taking, examination, ECG, Echo study and basic invasive cardiac electrophysiological procedure that include right - sided cardiac catheterization through the femoral veins and programmed pacing was performed after localization of the catheters in the heart using the standard stimulation protocol to induce the tachycardia. In addition, His bundle study which involve measurement of atrial - His (AH) interval, Ventricular - atrial time (VA) as a baseline and after tachycardia induction, demonstration of the AH jump and/or echo beat (ectopic) pre and post ablation of the slow pathway using radiofrequency energy. However this study was conducted in Leipzig Heart Center/Germany from January 2013 to February 2014. The result of this study reveals that 33 (84.6%) of the patients developed typical AVNRT and 6 (15.4%) with atypical AVNRT. 25 patients (64.1%) were females (23 (92%) typical type and 2 (8%) atypical type) and 14 patients (35.9%) were males (10 (71.4%) typical type and 4 (28.6%) atypical type). Among the patients group with typical AVNRT the 91.7% discovered to have two AV nodal pathways and 84.6% with three pathways in respect to 8.3% with two AV nodal pathway and 15.4% with three pathways within the patients group with atypical AVNRT. Accordingly, 38.5% of all patients in this study discovered to have multiple pathways. Concerning ablation and modification of the slow pathway, complete ablation is achieved in 59% while modification of the pathways was noticed in 41%. In conclusion, this study concludes that an invasive electrophysiological study is a safe and reliable method for identification of patients with high risk of tachyarrhythmias. Moreover, most of the female patients discovered to have typical AVNRT. Unusually, higher percent of patients developed multiple pathways (more than two pathways) with respect to the usual dual pathways. The disappearance of post ablation EP data (AH jump and/or echo beat) reflect successful complete slow pathways ablation whereas the appearance of these data considered being a modification of the slow pathway

دراسة التعبير الوراثي للجينات P53, KRAS, c - MYC & Her - 2/neu وانواع الرنا المايكريوية miR - 21, 34a, 92 & 98 على عينات ماخوذة من الغسول القصبي والانصباب الجنبي لمرضى مصابين بسرطان الرئة ومرضى مصابين بامراض رئوية مزمنة غير سرطانية == Expression of P53, KRAS, c - MYC, Her - 2/neu genes and microRNAs 21, 34a, 92 & 98 in cancerous and non - cancerous bronchial wash and pleural aspirate

Author name: حسين عبادي الجبوري
Supervisor name: بان عباس عبد المجيد | عدنان الجبوري
General topic: Medicine
Specific topic: Diseases
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Background : Lung cancer is the leading cause of death from cancer in men all over the world. Most of newly diagnosed cases were in advanced stage and beyond radical treatment due to late appearance of worrying symptoms and absence of effective screening method for high risk groups. Nowadays, depending on recent advances in molecular studies researches are directed toward finding molecular markers for diagnostic, therapeutic and prognostic purposes. Aim of study : To investigate the possibility of using expression of P53, KRAS, Her - 2/neu, c - MYC and microRNAs 21, 34a, 92, and 98 as a molecular biomarkers for detection of lung cancer in samples of bronchial wash and pleural fluid. Material and Methods : A prospective case control study on a total of 120 samples, sixty bronchial washes and sixty pleural effusions. The samples were taken from patients recruited at the Thoracic Surgical Unit in the Specialized Surgery Hospital/ Medical City during the period from March 2012 to April 2014. The work was performed in the Department of pathology and forensic Medicine, Baghdad college of Medicine. The specimens were thirty bronchial wash and thirty pleural fluid samples positive for lung cancer cells by cytopathology, and similar number of negative samples. Studied genes were amplified using qRT - Realtime PCR. Housekeeping genes for normalization of mRNAs was GAPDH and RNU - 48 for microRNAs. Expression was calculated using equation; Expression = (2 - ΔΔCt). Results : Results of Ct values for each marker were obtained from Max Pro 5000 Agilent Technology PCR software and raw and standardized Ct values were analysed using SPSS - 22 software. The mean, standard deviation, t - test, ANOVA test and LSD (least significant difference) were obtained before and after normalization. A statistically significant differences in the expressions of p53, KRAS, c - MYC, Her - 2/neu and microRNAs, 21, 34a, 92, & 98 genes were found between positive and negative (control) samples with a significant p - values of <0.05. Conclusion : According to this study a conclusion could be reached; the study of expression profiles of mRNAs of P53, KRAS, c - MYC, Her - 2/neu and microRNAs 21, 34a, 92 and 98 genes can be used as a biomarker in the detection of lung cancer, differentiating subtypes, and screening of high risk groups

استخدام الصبغة المناعية p16INK4a والتهجين الموقعي وتاثيرها على فحوصات عنق الرحم الصحيحة والملتبسة == The Use of p16INK4a and In Situ Hybridization and Their Impact on the Healthy and Equivocal Pap Smears of the Cervix

Author name: ثناء جميل الخشالي
Supervisor name: ندى صالح امين | هدى مهدي الخطيب
General topic: Medicine
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Throughout the past thirty years, the perception of cervicalcarcinoma has shifted from that of a mysteriously fatal disease, to one ofthe sexually transmitted, human papillomavirus (HPV) related infection.HPV DNA has been found in almost all cervical invasive and preinvasivecervical neoplasms.Conventional Pap smear, which was established in the early sixtiesreduced effectively the morbidity and mortality related to cervical cancer.Given the lower sensitivity of the Pap cytology test, new diagnosticparameters have been established. The histological features ofpreinvasive cervical neoplasia (CIN1, CIN2, and CIN3) are wellunderstood. However, misinterpretation of the morphological criteriacould lead to significant variability.The aim of this study is to identify women with equivocal and normalPap smear who are at risk for developing cervical cancer through the useof p16INK4a immunostaining and human papillomavirus (HPV) DNAtesting using in situ hybridization (ISH).Materials and MethodsIn order to identify human papillomavirus infection in minorcytological and histological abnormalities, biopsies collected from 60women 25 - 66 years of age with normal cervices and Pap smears (groupI), abnormal cervices and Pap smears (group II) and with cervicalcarcinoma (groupIII) referred to the Colposcopy Clinic in BaghdadTeaching Hospital - Medical City Complex, during the period from June2013 through July 2014. Cytology samples collected with ThinPrep forIVliquid base cytology (LBC), punch biopsy for histopathology formalinfixed and paraffin embedded. In situ hybridization (ISH) and p16INK4aimmunohistochemistry in addition to routine hematoxylin and eosin(H&E) stain were used to evaluate the histological specimens.ResultsThe age, age of marriage and parity of the three groups werestudied in relation to each of ISH and p16INK4a reactions. Agedistribution was highly significant among all age groups; in both ISH andp16 test results GI &GII (p=0.00), GI & GIII (p=0.00), GII &GIII(p=0.00) in both ISH and p16 test results. The age of marriage was notsignificant among the groups in both ISH and p16; GI & GII p (0.93), GI&GIII (0.12), GII &GIII (0.30). The effect of parity was insignificantamong all groups; GI & GII = (p = 0.66), GI & GIII (p = 0.82), GII &GIII (p = 0.79) in both ISH and p16 test resultsp16INK4a immunoreaction : Among group I there was a negativereaction in all the specimens collected. In specimens from group II, therewas a very mild or focal p16INK4a immunoreaction in one specimen(5%), a moderate immunoreactivity in 9 specimens (45%), and a highpositive reactivity in 10 specimens (50%) (p=0.000). Group III showedvery high immunoreaction in all the specimens collected.In situ hybridization (ISH) in group I was mild positive in 4specimens (20%) which showed diffuse signal pattern. A mild positivereaction with diffuse nuclear staining was noticed in 4 (20%) of thecervical specimens. Moderate positive reaction with diffuse staining ofthe nucleus was observed in 8 (40%) of the specimens. The remaining 8(40%) of the specimens showed both diffuse and punctate staining of theVnuclei. Among group III specimens, the nuclei in the epithelial cellsshowed both punctate and diffuse signal patterns.Chi square was done to compare results concerning ISH & variousgroups was highly significant (p= 0.00) ConclusionHPV infection is prevalent and can be missed by the conventional Pap smear. The severity of the disease was directly proportional with the age of women in this study. p16 overexpression was correlated with the positivity of ISH. This may support the use of either, or both, tests to improve the accuracy of cervical intraepithelial neoplasia (CIN) diagnosis, and help in the triage of women with equivocal lesions.

تاثير قطر دوالي الخصيه على نوعية السائل المنوي للمرضى المصابين بدوالي الخصية غير السريري == The Impact of the diameter of spermatic veins on Seminal fluid Analysis of patients with Subclinical Varicocele

Author name: ابراهيم جاسم حمادي
Supervisor name: غسان ثابت سعيد | رعد حفظي توفيق
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: ان الصحة الانجابية هي انعكاس للحالة الصحية العامة والتي تشمل الصحة العقلية والفيزيائية والاجتماعية وليس مجرد مجموعه الامراض المتعلقة بالجهاز التناسلي ووظائفه وعليه يعد العقم مرضا يستحق التشخيص والعلاج . يعرف العقم على انه عدم القدرة على الانجاب بعد سنة من الزواج بدون وجود اي مانع للعلاقة بين الزوجين . يشمل العامل الذكري حوالي 25 - 30 % من كل حالات العقم وايضا 30% اخرى بالاشتراك مع العامل الانثوي . ان 60% من عقم الرجال ناتج عن عوامل جينية اما الاسباب الاخرى فقد تكون نتيجة لدوالي الخصيتين ، عوامل بيئية ، عقاقير طبية ، تعاطي الكحول او التعرض المستمر للحرارة العالية او المواد الكيميائية....الخ. يعد مرض دوالي الخصيتين من الامراض الشائعة بين الرجال الذين يعانون من العقم وهو عبارة عن توسع وتعرج الاورده الناقلة للدم الراجع من الخصيتين نتيجة لتلف الصمامات الوريدية وينعكس ذلك سلبا على الوظيفة الطبيعية للخصية نتيجة لارتفاع درجه حرارة الخصية بسبب تراكم الفضلات الكيميائية من الغدد فوق الكلوية وكذلك الكليتين اضافة الى اسباب اخرى. يشكل دوالي الخصية السريري حوالي 15% بين الرجال ويشخص سريريا بفحص الخصيتين . اما في حالات اخرى لايمكن تشخيص دوالي الخصيه عن طريق الفحص السريري فقط وعليه تستخدم وسائل اخرى للتشخيص حيث يتم استخدام اجهزه الفحص بالموجات فوق الصوتية (السونار) لغرض تشخيص حالات كهذه ويسمى الدوالي غير السريريه وهو موضوع البحث . اجريت الدراسة على 122مريضا في عيادة علاج العقم في مستشفى اليرموك التعليمي بغداد/العراق. وقد استثني من الدراسة اي مريض مصاب باي مرض ممكن ان يتعارض مع الخصوبة وقد اخذت معلومات عامة من المرضى كذلك تم قياس الطول والوزن وقد اجريت لهم بعض التحاليل المختبرية كتحليل السائل المنوي وقياس مستوى بعض الهرمونات في الدم والتي شملت الهرمون المحفز للحويصلات((FSH,هرمون الجسم الاصفر (LH) وهرمون الشحمون الذكري(Testosterone) .كذلك تم فحص السائل المنوي للتاكد من وجود جينFasL)) لمجموعه تتكون من 60 مريضا والذين يعانون من الدوالي غير السريريه في مختبرات جامعة مانشستر مترو بوليتان في المملكة المتحدة. قسم المرضى الى مجموعتين الاولى تشمل المرضى المصابين بالدوالي السرير ي والمجموعة الثانية وتشمل المرضى المصابين بالدوالي غير السريري وقد تم مقارنه النتائج مع 50 مريضا سليما وقادرا على الانجاب . لقد اجريت عمليات جراحيه لعلاج الدوالي غير السريريه ل 60 مريضا وملاحظه التغيرات التي قد تطرا على متغيرات السائل المنوي والهرمونات وكذلك حجم الخصية . اوضحت الدراسة تاثيرا سلبيا بين المرضى الذين يعانون من الدوالي السريريه ومتغيرات السائل المنوي حيث كان التاثير واضحا في قله اعداد الحيوانات المنوية وكذلك سرعتها وشكلها الطبيعي كما اثر سلبا على حجم الخصيه المصابة وكذلك اظهر الفحص زيادة معنويه في هرمون (FSH) ونقص واضح في الهرمون الذكري (T).دون التاثير على هرمون LH)). اظهرت الدراسة تاثير دوالي الخصية غير السريري على متغيرات ا لسائل المنوي حيث كان التاثير على عدد الحيوانات المنوية وكذلك على سرعه الحيوانات المنوية وعلى شكلها الطبيعي دون التاثير على حجم الخصية او على هرمونات FSH),) او LH)).مع تاثير معنوي واضح على هرمون التستوستيرون(T) وكانت نتائج فحص ال FasL))مرتفعه لدى مرضى المجموعه. يستنتج من هذه الدراسة ان دوالي الخصية غير السريريه له تاثير واضح على بعض متغيرات السائل المنوي والهرمون الذكري وان اجراء العملية الجراحية لبعض المرضى يساعد في تحسن مستوى الخصوبة لديهم | Reproductive health is a state of complete physical, mental and social well - being and not merely the absence of disease or infirmity in all matters relating to the reproductive system and to its functions and processes. Infertility should, therefore considered to be a disease process worthy of investigation and treatment. Infertility defined as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factors alone constitute about 25% - 30% of all cases of infertility, and they contribute to another 30% in combination with female factors and about 60% of male factor infertility may be due to genetic causes, others could be due to varicocele, genitourinary infections, environmental agents drugs, alcohol, life style factors like age and obesity. Varicocele is a state of dilated, elongated and tortuous veins of the pampiniform plexus of the spermatic cord typically developed during adolescent and have been found in about 15٪ of the general populations This condition is pathophysiologically characterized by retrograde flow in testicular veins found clinically as the presence of a palpable, soft scrotal mass, could be associated with mild to moderate pain, and they can also be associated with infertility. The size of varicocele is variable according to the severity of the disease. It ranges from large palpable mass of veins easily felt by the patient to varicocele that is only discovered by ultrasound study called subclinical varicoele. The treatment of the large sized varicocele is recommended and known to cause relief of the symptoms and might cause improvement to the seminal fluid quality and the state of fertility while the subclinical varicoele treatment is still controversial as there are results of some studies that showed a significant improvement of fertility after surgical treatment otherwise the treatment in many centers is still not indicated. The study objectives were to determine the relationship between subclinical varicocel and male fertility parameters in addition to follow up treated subclinical patients to determine the effects of surgical treatment on the seminal fluid and fertility. Data analyzed for 122 patients included in this study , full comprehensive history was taken ,physical examination ,testicular color Doppler ultrasound examination , pre and post operative assessments of seminal fluid analysis also evaluation serum levels of some reproductive hormonal profile includes, Follicle stimulating hormone (FSH), Luteinizing hormone (LH) and Testosterone (T) in addition to the expression of FasL mRNA gene in seminal sperms of the patients with subclinical varicocele . The Doppler ultrasound was done to all included patients to measure the testicular volume and the accurate size of the dilated testicular veins to diagnose the subclinical cases , the veins diameter 2.5 mm was used as a cutoff point. Follow up was done for 60 patients of subclinical group pre and post varicocelectomy .Fifty healthy fertile male of similar age were included in this study as a control group.The study was done in male infertility clinic of Al - Yarmouk Teaching Hospital Baghdad, Iraq from the 1st of October 2013 to the end of December 2014 while the FasL expression of 60 samples of subclinical varicocele patients were studied in Manchester Metropolitan University . Health and science laboratories - UK. Results showed that the subclinical varicocele affects male fertility with a significant negative correlation between subclinical group and some of semen parameters including : sperm morphology, percentage of progressive motile sperms and also a significant positive correlation between sperm count and FasL gene. The results also supported the hypothesis of clinical varicocele effects on male fertility as there was a significant negative correlation with the total sperm count, percentage of progressive motile sperms and also there was a significant increase in FSH with decreased testosterone (T) and mild decrease in the testicular volume. From this study we can conclude that subclinical varicocele cause decrease in sperm count, motility and sperm morphology and to some extend hormonal serum levels but no significant changes for testicular volume. Samples of subclinical patients showed a significant expression of FasL RNA and the surgical treatment of such cases gave an improvement to the state of fertility.

دالات بيوكيماوية جديدة للساركوبينيا والضعف العضلي لكبار السن في العراق العام الدراسي 2016 - 2017 == Novel Biochemical Markers for Sarcopenia and Muscle Frailty in Iraqi Elderly

Author name: ولاء اسماعيل جاسم
Supervisor name: هدف ظافر الياسين | نزارعبد اللطيف
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: تعرف الساركوبينيا بانها النقصان التدريجي في قوة وكتلة العضلات الهيكلية وهذا يحصل بتقدم العمر. الساركوبينيا اما ان تكون ابتدائية سببها تقدم العمر فقط بدون وجود اي سبب اخر او ثانوية وسببها تقدم العمر الذي له علاقه بالفعالية , الامراض ولتغذية. العينات وطريقة العمل تم اخذ مجموعه عينات دم من اشخاص يرتادون المستشفى التعليمي في بغداد/قسم الروماتولوجي خلال الفترة من الاول من ايلول 2016 الى نهاية شباط 2017. تضمنت الدراسة 100 عينه لاشخاص يعانون من الساركوبينيا(50 امراه و50 رجل) و50 يبدون اصحاء كمجموعة سيطرة(25 امراه و25 رجل). اخذت المعلومات من جميع الاشخاص وقد تضمنت الجنس والعمر والاصابة بالامراض. ( استبعدالا شخاص الذين يعانون من الروماتيزم الرثوي, داء الذئبة الاحمراري، مرض السكري، امراض الغده الدرقية والاشخاص الذين يتعاطون ادويه تحتوي على ستيرويدات). تم تشخيص الاشخاص الذين يعانون من الساركوبينيا الابتدائية بواسطة اختبار الاداء الحركي لكبار السن وجهاز فحص هشاشة العظم (لتعيين مجموع كتلة عضلات الجسم وكتلة عضلات الهيكل العظمي، ومعامل كتلة الجسم. ). اجريت بعض التحاليل البيولوجية التي تضمنت تحاليل تخص الالتهابات مثل الانترلوكين - 6 والبروتين سي عالي الفعالية والالفا 1 انتي - كيموتربسين ولقياس تليفات العضلات (البروكولاجين تايب 3 ان ترمنل ببتايد) ولقياس التقاطع العضلي العصبي ( تركيز السي اكارين فركمنت )ولقياس نمو العضلات تم قياس المايوستاتين.كل المتغيرات البيولوجية قيست بواسطة استخدام تقنية الالايزا. النتائجمعدل قيم كل من كتلة عضلات الجسم والهيكل العظمي والالفا 1 انتي كيموتربسين في مجموعة السيطرة اعلى مما في مجموعة الساركوبينيا وفي الرجال اعلى من النساء وجميع القيم تقل بتقدم العمر. معدل قيم معامل كتلة الجسم ,الانترلوكين - 6 والبروتين سي عالي الفعالية، البروكولاجين تايب 3 ان ترمنل ببتايد وقياس تركيز السي اكارين فريكمنت والمايوستاتين في مجموعة السيطرة اقل من المجموعة المصابة بالساركوبينيا وفي النساء اعلى من الرجال وتزداد القيم بتقدم العمر. هنالك فروقات معنويه عالية في قيم جميع المتغيرات P≤0.01 بين مجموعة السيطره ومجموعة الساركوبينيهاما بالنسبة للفروقات المعنوية حسب الاعمار فهناك فروقات معنويه عالية بين كل المتغيرات البيولوجية P≤0.01ما عدا المايوستاتين حيث لا توجد فروقات معنويه بين قيمه P≥0.05اما بالنسبة للعلاقة بين المتغيرات اعتمادا على الجنس فهي ذات فروقات معنويه عالية P≤0.01 ماعدا قيم الفا 1انتي - كيموتربسين والسي اكارين فركمنت حيث كانت الفروفات معنويه P≤0.05 كما لم تكن هنالك فروقات معنويه بين البروكولاجين تايب 3 ان ترمنل ببتايد والسي اكارين فركمنت P≥0.05 المناقشه1 - معدل قيم كل من كتلة عضلات الجسم وكتله عضلات الهيكل العظمي والالفا 1 - كيموتربسين في مجموعة السيطرة اعلى مما في مجموعة الساركوبينيا وفي الرجال اعلى من النساء وجميع القيم تقل بتقدم العمر. السبب في ذلك قلة الالياف العضليه , قلة خلايا وحدة الحركه , قلةخلايا الارسال وزيادة كتلة الدهون في الاشخاص المصابين بالساركوبينيا. 2 - معدل قيم كل من الانترلوكين - 6 والسي بروتين عالي الفعالية يقلل من قوة العضلات في حين ان الالفا 1 - كيموتربسين يزيد من قوة العضلات عند الاشخاص المصابين بالساركوبينيا. والسبب في ذلك ان الالفا 1 - كيموتربسين تحمي العضلات من التلف اثناء الالتهابات ولكن زيادة معدل قيمة الانترلوكين - 6 يثبط عمل الالفا1 - كيموتربسين . وتزداد قيم السي بروتين عالي الفعاليه والانترلوكين - 6 بتقدم العمر وفي الرجال اكثر من النساء بسبب زيادة كتلة غضلات الجسم وكتلة عضلات الهيكل العظمي .3 - معدل قيم السي اكارين فركمنت في مجموعة الساركوبينيا اعلى منه في مجموغة السيطرة والسبب في ذلك عند اعادة بناء الخلايا العصبيه والعضليه عند كبار السن او المصابين بالساركوبينيا الاكارين ينقسم بواسطة انزيم الكيموتربسين ويطرح السي اكارين فركمنت الى الدم وتزداد فعالية الكيموتربسين وتقل قوة الخلايا العضليه والعصبيه.4 - معدل قيم البروكولاجين تايب 3 ان ترمنل ببتايد في مجموعة الساركوبينيا اعلى من مجموعة السيطرة والسبب انه خلال عملية اعادة بناء الخلايا العضليه العصبيه البروكولاجين تايب 3 ان ترمنل ببتايد قيمها ستزداد بسبب قلة الالياف العضلية وكذلك عملية ازالة التعصب مما يؤدي الى ضعف في الكتله العضليه.5 - المايوستاتين ينظم نمو العضلات سلبيا. المايوستاتين اساسا يثبط عمليه تصنيع المايوستاتين وعندما يزداد تركيز المايوستاتين يقل نمو وقوة العضلات.6 - في الاشخاص المصابين بالساركوبينيا معامل كتلة الجسم اعلى من مجموعة السيطرة. زيادة الدهون ووزن الجسم سببها هو قلة الاحتياج الى الطاقه, قلة فعالية الجسم, قله معدل الايض القاعدي واستخدام كمية سعرات اكثر من التي يحتاجها الجسم.7 - اكثر العوامل التي تؤثر على الساركوبينيا تباعا هي كتلة عضلات الجسم الهيكليه,كتلة عضلات الجسم, المايوستاتين, البروكولاجين تايب 3 ان ترمنل ببتايد , السي اكارين فركمنت ثم الالفا 1 - كيموتربسين. | Sarcopenia is defined as the progressive decline in skeletal muscle mass and strength that occurs with aging. Sarcopenia either primary which is age - related and with no other cause except ageing or secondary sarcopenia which is related to activity, disease and nutrition.Subjects and methodsA cross sectional, case - control study was done in Rheumatology Clinic in Baghdad Teaching Hospital (during the period from September first 2016 to the end of March 2017). It included 100 sarcopinic subjects (50 male and 50 female) and 50 non - sarcopinic subjects (25 male and 25 female). Information was taken from each subject including age, & gender and diseases. Patients with inflammatory diseases (rheumatoid arthritis, systemic lupus erythematosus), diabetes mellitus, thyroid disease and patients taking steroid therapy were excluded.Subjects with primary sarcopenia in this study were diagnosed by : Short Physical Performance Battery and dual - energy x - ray absorptiometry. appendicular skeletal muscle mass, total lean body mass and body mass index.The biomarkers studied were : inflammation : (interleukin - 6, hs - C - reactive protein and α1 - antichymotrypsin ), for markers of muscle fibrosis : (procollagen type III N - terminal peptide, for markers of functional neuromuscular junctions C - terminal Agrin fragment and myostatin

دراسة المؤشر ات الحيوية للخلايا الجذعية السرطانية FOXM1, TAZ, EpCAM,CD47 في ما يتعلق بالمعلمات النسيجية لسرطان الثدي

Author name: رفاه محمد جعفر الخطیب
Supervisor name: سالم رشید حمودي العبیدي
General topic: Medicine
Specific topic: Tissue Diseases
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

دراسة جزيئية للطفرتين NPM1 - A وFLT3 - ITD مع التعبير النسخي للمورث الجزيئي FLT3 في نموذج من المرضى العراقيين البالغين المصابين بابيضاض الدم النقياني الحاد وعلاقتهما مع المؤشرات السريرية والمختبرية == Molecular Study of NPM1 - A, FLT3 - ITD Mutations and FLT3 Transcript Expression in a Sample of Iraqi Adult Acute Myeloid Leukemia Patients : Their Correlations with Clinicopathological Parameters

Author name: شيماء محمد عبد اللطيف
Supervisor name: ختام رزاق كاظم الخفاجي | علي محمد جواد
General topic: Medicine
Specific topic: Diseases - Blood
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Acute myeloid leukemia is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and extension of leukemic cells blocked at the early stage of hematopoiesis. It has a great variability in the pathogenesis, clinical features, and treatment outcomes. Detection of molecular markers has become a smart tool to further division of patients in acute myeloid leukemia subgroups.Nucleophosmin1 mutations are found in approximately 30% of adult acute myeloid leukemia patients and are associated with a good outcome when detected in absence of duplications in the Fms - like tyrosine kinase 3 gene. Over 50 molecular Nucleophosmin1 mutation variants have been recognized; the most common one is Nucleophosmin1 - A mutation.The Fms - like tyrosine kinase 3 receptor is expressed on the surface of hematopoietic stem cells and plays a vital role in normal hematopoiesis. Numerous studies have revealed that high levels of Fms - like tyrosine kinase 3 were expressed in patients with acute myeloid leukemia. Fms - like tyrosine kinase 3 - Internal tandem duplication mutations are found in around 20 - 25% of patients with acute myeloid leukemia and are associated with increased transcript level of Fms - like tyrosine kinase 3 and with a poor scenario in adult acute myeloid leukemia patients.Aim of the Study1. Detect the frequency of Nucleophosmin1 - A; Fms - like tyrosine kinase 3 - Internal tandem duplication along with assessment of Fms - like tyrosine kinase 3 transcript expression in a sample of newly diagnosed Iraqi adult acute myeloid leukemia patients.2. Study the relationship of Nucleophosmin1 - A, Fms - like tyrosine kinase 3 - Internal tandem duplication mutations and Fms - like tyrosine kinase 3VItranscript expression with various clinicopathological parameters and French - American - British subtypes of the disease as well as the correlation among the three markers.Materials and MethodsThis is a cross - sectional study, conducted during the period extending between April 2015 and September 2016. The bone marrow aspirate samples of 53 adult acute myeloid leukemia patients were collected at the time of diagnosis and prior to treatment at the Hematology Ward of Baghdad Teaching Hospital in Medical City compared with 53 control individuals. All the control bone marrows obtained from patients with anemia and idiopathic thrombocytopenic purpura and were negative for infiltrative lesions. The related clinical and laboratory data for each patient were registered at the time of diagnosis.The study was conducted at Main Laboratory of Baghdad Teaching Hospital/ Medical City, Clinical and Communicable Diseases Research Center/College of Medicine/ University of Baghdad and Postgraduate Laboratory of Pathology and FoThe RNA was extracted and was reverse transcribed into cDNA. Real time polymerase chain reaction technique was applied on bone marrow aspirate samples of acute myeloid leukemia and control groups to detect the frequency of Nucleophosmin1 - A mutation, Fms - like tyrosine kinase 3 expression using a TaqMan probe and SYBR green assays respectively and detection of Fms - like tyrosine kinase 3 - Internal tandem duplication mutation using gel electrophoresis post polymerase chain reaction procedure

دراسة تجريبية لانزيمات البروتييز للزائفة الزنجارية المعزولة من المرضى العراقيين المصابين بتقرح القرنية ودورها في علاج تقرح القرنية المتسبب عن المكورات العنقودية == Experimental Study of Pseudomonas aeruginosa Proteases Isolated from Corneal Ulcer of Iraqi Patients and Their Role in the Treatment of Staphylococcus aureus Keratitis

Author name: عائدة حسين ابراهيم
Supervisor name: جاسم محمد كرحوت | منيرة جلوب اسماعيل
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: One - hundred and twenty samples ( corneal scraping) were collected from patients diagnosed to have microbial keratitis (corneal ulcer) who attended Ibn Al - Haitham Teaching Eye Hospital from the period between May 2013 and November 2013, Pseudomonas aeruginosa was reported 26 (21.6%) from the total cases. All bacterial isolates were diagnosed by conventional and biochemical tests, and confirmed by Vitek 2 Compact System.The role of proteases enzymes ( Elastase ( LasB), LasA, Alkaline protease and Protease IV ) of Pseudomonas aeruginosa in the corneal ulcer was studied by using genetic and molecular biological method by real time PCR, and the results indicated that three bacterial isolates of Pseudomonas aeruginosa possessed elastase gene (LasB) (11.5%), and only one bacterial isolate of Pseudomonas aeruginosa harbored LasA protease gene ( 3.8%). All bacterial isolates of Pseudomonas aeruginosa were harbored alkaline protease gene (100%), and twenty bacterial isolates were harbored protease IV (76.9%).The results of real - time PCR analysis indicated that four bacterial isolates of Pseudomonas aeruginosa (15.3%) were harbored more than one gene of different proteases enzymes ( elastase, alkaline protease, and protease IV).On the other hand our results showed that one bacterial isolates (3.8%) harbored both LasA protease and alkaline protease genes, and twenty bacterial isolates of Pseudomonas aerugenosa (76.9%) were harbored alkaline protease and protease IV genes.The LasA protease was extracted from Pseudomonas aeruginosa isolate by cooling centrifuge and precipitated supernatant by ammonium sulfate at saturation (80%). The resulted extracted crude enzyme concentration was 60 μg/ml. Then the crude enzyme was partially purified by dialysis and gel filtration chromatography by using Sephadex G - 100. The concentration of partial purified enzyme reached 40μg/ml.IIISummaryThe results of the experimental treatment of bacterial keratitis ( in vivo) of infected eyes rabbits caused by Methicillin Sensitive Staphylococcus aureus showed that the efficacy of LasA protease was effective was as Lysostaphin in eradicating Methicillin Sensitive Staphylococcus aureus from the infected corneas after approximately 15 h after giving the drug at dose 100 μl ( concentration 1μg / ml ) . While Vancomycin gave us very little potency in eradicating S. aureusfrom corneas in comparison with potency of LasA protease and Lysostaphin duringthis time but showed good potency very late approximately after 3 days of applicationof treatment.The results of the experimental treatment in vitro (in the test tube ) that is caused by Methicillin Sensitive Staphylococcus aureus showed that the efficacy of LasA protease was similar to that of Lysostaphin drug in the killing of Methicillin Sensitive Staphylococcus aureus in the bacterial broth.

تاثير الفيتامينات B1,B6 and B12) على الاعتلال العصبي المحيطي : دراسة نسيجية، نسيجية كيميائية وكيميا نسيجية مناعية == The effect of vitamins B1, B6 and B12 on peripheral neuropathy , Histological , Histochemical and Immunohistological studies

Author name: صالح مهدي علي
Supervisor name: معن حمد الخالصي
General topic: Medicine
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Peripheral neuropathy is a nerve injury process by disease or so on leading to damage of peripheral nervous system . Diabetes mellitus and compression are most common causes of peripheral neuropathy. Vitamins (B1,B6 and B12) commonly used for treatment of peripheral neuropathy. The objective of this study is to compare the effect of vitamins (B1,B6 and B12) on nerve regeneration and recovery after induced sciatic nerve crush injury in experimental animal model.Seventy five male Swiss albino rats were divided randomly into five groups and each group consisted of 15 animals. Group A( which considered as control positive(with crush injury) received normal saline intramuscularly for 45days. Group B received vitamin B1 , group C received vitamin B6 , group D received vitamin B12 and group E which considered as control negative(no crush injury and no treatment) . All animals were injected vitamins intramuscularly for 45days. Tail flick test time was reduced in rats treated with vitamin B12 after crush injury to the Sciatic nerve. Mid thigh circumference (MTC) was significantly increase in B12 treated group after crush injury to the Sciatic nerve. Data analysis was performed by SPSS 20 software using descriptive statistics.IVHistological examination of rats sciatic nerves stain by H&E on day 15 showing degenerative nerve fibers with Schwann cell infiltration in all groups but it is less prominent in group D. On day 45 histological examination of rats gastrocnemius muscles exhibited separation of bundles muscle fibers by connective tissue, variation of fibers in size and shape with atrophy of muscle bundles in all groups .While these were less prominent in rats treated with B12.Van Giesons staining of rat sciatic nerves on day 45 after crush injury. In all groups experimental and control (B,C,D,A) showing degenerative nerve fibers in different areas but less in group D and better organized sciatic nerve fibers in group D.Finally, Immunohistochemical study of (anti S100 protein) of rat sciatic nerves on day 45showed strong activity for those rats treated with vitamin B12 while rats treated with vitamin B1 and B6 exhibited moderate and weak activities respectively .In conclusion vitamin B12 promote peripheral nerve regeneration more than vitamin B1and vitamin B6.

العلاقة مابين بعض العلامات الحيوية القلبية الحديثة مع درجة التكلس والتصلب في الشرايين التاجية المقاسة : متابعة علاجية بالستاتين == Association of Some Novel Biochemical Markers with Coronary Artery Calcium Score and Stenosis : Follow Up treatment with Statin

Author name: نور نصير نافع
Supervisor name: باسل عويد محمد صالح | صباح موسى فاضل
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: يعد مفراس شرايين القلب القسطاري من احدث الطرق لتشخيص امراض الشرايين التاجية من خلال التصوير المقطعي (CCTA ) للاوعية الدمويه التاجية، والذي يعد من احدث الطرق التي تطورت بشكل ملحوظ خلال العقد الماضي من خلال تلوين الشرايين التاجية بدون تدخل جراحي لتشخيص المرض وبدقة عالية تتناسب درجة مقياس التصلب (Ca - Score) مع شدة التصلب الشرايين التاجية ويحدد درجة تطور المرض فى المراحل الاولى، ويعد الفتيون اي (Fetuin - A) من احدى العوامل السلبية الحادة المتفاعلة الذي لايزال غير معروف من الناحية الوظيفية الفسلجية، وهنالك العديد من الدلائل والدراسات التي توضح دوره المهم في عملية تكلس الاوعية الدموية الاوستيونكتين (Osteonectin) هو بروتين سكري لاصق ويعتبر عامل نضير صماوي تم تشخيصه حديثا من خلال انعكاس دوره في الحالة المرضية للانسجة الدهنية ، مثل اعادة تجدد الاوعية الدموية. ويعد الفسفاتين (Visfatin) نوع من انواع الاديبوسايتوكينات المتعدد الاوجه والذي يلعب دورا مهما في العمليات الايضية من خلال الجزء الخارجي من تركيبه وكذلك دوره في مختلف الاثار الضارة على الاوعية الدموية من خلال الالتهاب والانتشار . وقد برز حديثا دور البنتراكسين 3 (Pentraxin 3) كعلامة جديدة يعتقد انها اكثر تحديدا في التهاب الاوعية الدموية من البروتينات الاخرى في عائلة البنتراكسين مثل ارتفاع البروتين سي الحاد التفاعلي (hs - CRP).الهدفلدراسة العلاقة مابين العلامات الحيوية القلبية الحديثة الفتيون اي والاوستيونكتين والفسفاتين والبنتراكسين 3 والبروتين سي الحاد التفاعلي(hs - CRP)(Pentraxin 3)(Visfatin)(Osteonectin)(Fetuin - A) في المرضى الذين يشتبه باصابتهم بامراض شرايين القلب التاجية الذين لديهم درجه خفيفة الى متوسطة من تكلس الشرايين التاجية ، ثم مقارنة النتائج مع المرضى للذين يشتبه باصابتهم بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية وايضا لدراسة دور هذه العلامات الحيوية الحديثة مع درجة التكلس (Ca - Score) ودرجة السمنة (BMI) ومستويات الدهون في الدم المتمثلة بقياس (Cholesterol,HDL,TG) ثم دراسة تاثير علاج الدهون الـ (Statin, atorvastatin 10 - 40 mg ) على مستوى كل من (العلامات الحيويه القلبية الحديثة ومستوى الدهون ) في المرضى المصابين بامراض تصلب شرايين القلب التاجية الذين يعانون من درجة تكلس خفيفة الى متوسطة ( Ca - Score =1 - 399 Agtaston score) .المواضيع اجريت هذه الدراسة في فرع الكيمياء الحياتية في كلية الطب - جامعة بغداد في المختبرات التخصصية لمستشفى ابن البيطار التخصصي لامراض القلب والشرايين خلال الفترة الممتدة من شباط 2013 الى كانون الاول 2013 من خلال تضمين 200 مريض مصاب بامراض تصلب شرايين القلب التاجية ثم ادراج 65 من المرضى المصابين بامراض تصلب شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية ولا يستخدمون علاج لدهون (Statin, atorvastatin 10 - 40 mg ) تم تشخيص شدة التكلس بالشرايين التاجية من خلال قياس درجة التكلس من خلال جهاز فليبس للانظمة الطبية 64 بريليناس . ثم تقسيم المرضى الى 65 مريضا وعلى ثلاثة اقسام بحسب درجة التكلس لديهم والى ثلاثة مجاميع : المجموعة الاولى تضمنت 20 مريض لديهم درجة تكلس تساوي صفر وتتراوح اعمارهم مابين (31 - 65) سنة، والمجموعة الثانية ذو درجة تكلس خفيفة الى متوسطة ( Ca - Score1 - 399) تتضمن (25) مريض وتتراوح اعمارهم مابين (47 - 74)سنة، والمجموعة الثالثة ذو درجة تصلب شديدة ( Ca - Score ≥400) وتراوح اعمارهم مابين (49 - 75) سنة. تم متابع تاثير علاج الدهون (Statin, atorvastatin 10 - 40 mg ) على مستوى العلامات الحيوية القلبية الحديثة ومستوى الدهون لدى(20) مريض من اصل (25) من المجموعة الثانية تحت اشراف الطبيب الاختصاص في امراض القلب (د.صباح موسى فاضل).النتائجكشفت نتائج هذه الدراسة انخفاض كبيرذو دلالة احصائية في مستويات تركيز كل من : (الاستيونكتين والفسفاتين) مقرون بارتفاع ملحوظ ذو دلادلة احصائية في مستوى تركيز فيتيون اي فضلا عن الارتفاع غير الملحوظ احصائيا في مستوى كل من (البنتراكسين والبروتين سي الحاد التفاعلي) في المرضى المصابين بامراض تصلب الشرايين التاجية المجموعة الثانية والثالثة ذو درجة تكلس الخفيفة الى شديدة مقارنة بالمرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية.وقد اظهرت هذه الدراسة وجود ارتباط سلبي ذو دلالة احصائية لدى المرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية بين الفسفاتين والبنتراكسين3 بينما لوحظ وجود ارتباط سلبي ذولالة احصائية بين الفسفاتين والاوستيونكتين في المرضى بامراض تصلب الشرايين التاجية المجموعة الثانية ذو درجة تكلس الخفيفة الى المتوسطة ولوحظ ايضا وجود ارتباط سلبي ذو دلالة احصائية مابين البنتراكسين والمقياس درجة السمنة من جهة في المرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية مع وجود ارتباط ايجابي ذولالة احصائية مابين البنتراكسين 3 والبروتين سي الحاد التفاعلي لدى المرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية مقارنة بالمرضى المصابين بامراض تصلب الشرايين التاجية المجموعة الثانية والثالثة ذو درجة تكلس خفيفة الى شديدة .وكذلك اظهرت هذه الدراسة ايضا وجود ارتباط سلبي ذو دلالة احصائية مابين الاوستيونكتين والكلوكوز لدى المرضى المصابين بامراض تصلب الشرايين التاجية للمجموعة الثالثة ذو درجة تكلس شديدة بينما وجد ارتباط سلبي ذو دلالة احصائية مابين الفتيون اي ومقياس درجة السمنة من جهة ومابين الفتيون اي ومستوى الكلوكوز من جهة اخرى لدى المرضى المصابين بامراض تصلب الشرايين التاجية ذو درجة تكلس خفيفة الى شديدة فضلا عن وجود ارتباط ايجابي ذو دلالة احصائية مابين البروتين سي الحاد التفاعلي ونسبة مستوى الكلكوز التراكمي بالدم مقارنتا بوجود ارتباط ايجابي ذو دلالة احصائية مع الكلسترول المفيد في الدم في المرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية بينما لوحظ وجود ارتفاع ملحوظ في كل من ( قيمة نسبة مستوى الكلكوز التراكمي ومستوى الدهون الثلاثية) ومستوى كل من ( (non - HDL ,AIفي المرضى المصابين بامراض تصلب الشرايين التاجية المجموعة الثانية ذو درجة تكلس خفيفة الى شديدةو المجموعة الثالثة ذو درجة تكلس شديدة مقارنة بالمرضى المصابين بامراض شرايين القلب التاجية الذين لايعانون من وجود تصلب في الشرايين التاجية. وقد ظهرت هذه الدراسة دور علاج الدهون في انخفاض مستوى كل من الفتيون اي والبنتراكسين3 وزيادة مستوى الدهون المفيد للمرضى المصابين بامراض تصلب الشرايين التاجية المجموعة الثانية ذو درجة تكلس خفيفة الى المتوسطة بعد متابعتهم لمدة تتراوح مابين (6 - 8) شهور. ولوحظ وجود علاقة طردية مابين هذه العلامات القلبية الحيوية الحديثة مع( درجة التصلب وعدد الاوعية القلبية التي حدث فيها التصلب )مع وجود ارتباط مابين درجة التكلس المقاسة من خلال جهاز المفراس مع نسبة التصلب .الاستنتاجنظرا لكون درجة تكلس الشريان التاجي ذو علامة بديلة متزامنة مع امراض تصلب الشرايين التاجية لوحظ تزامن حدوثها مع ارتفاع مستوى الفتيون اي مقارنتا بانخفاض مستوى كل من الاوستيونكتين والفسفاتين مما جعلني اقترح دور هذه العلامات الحيووية القلبية الحديثة في تطور الناحية المرضية المسببة لتصلب الشرايين التاجية على الرغم من ارتفاع مستوى كل من البنتراكسين 3 والحاسة البروتين سي التفاعلي والتي تعكس الدور المهم للالتهاب كعامل اولي وسيط لتقدم المرض. ثم لوحظ دور علاج الدهون بخفض مستوى كل من الفتيون اي وتابنتراكسين 3من خلال (Pleotropic effect ) خلال فترة متابعة (20) من اصل( 25) من المرضى المصابين بامراض تصلب الشرايين القلبية التاجية ذو درجة تكلس خفيفة الى متوسطة لمدة (6 - 8) اشهر. | Coronary Computed Tomography Angiography (CCTA) is a rapidly growing, noninvasive imaging modality that developed quickly over the last decade, and its role for evaluation of Coronary Artery Disease (CAD ) becomes of great promise with high diagnostic accuracy. The presence and extent of Coronary Artery Calcification (CAC) correlates with the overall magnitude of coronary atherosclerotic plaque burden and with the development of subsequent coronary events. Human Fetuin A, is negative acute - phase reactant, its major physiologic functions are not fully understood. Several lines of evidence have suggested that it may play an important role in blood vessel calcification. Osteonectin is an adhesive glycoprotein; it is a newly identified autocrine/paracrine factor that could affect key functions in adipose tissue physiology and pathology through its implication in pathological condition of adipose tissue such as vascular remodelling. Visfatin is an multifaceted adipocytokine, It’s circulating levels are enhanced in different metabolic disease; extracellular part can exert various deleterious effects on vascular cells, including inflammation and proliferation. The Pentraxin - 3 (PTX3) has emerged as a novel marker thought to be more specific to vascular inflammation than other proteins in the PTX3 family such as high sensitive C - reactive protein (hs - CRP). Objective To study the relationship between novel cardiac biomarkers; Fetuin - A, osteonectin , visfatin, PTX3 and hs - CRP in patients with suspected CAD who have mild to severe degree of coronary artery calcification, Ca - Sore (1 - ≥400 ASU),then compare the results to patients with suspected CAD without calcification (Ca - Sore =zero ASU)and to explain the important role of these novel biomarkers in development of CAC , also to the study association of Coronary calcium score and novel biomarkers with obesity markers(BMI), and parameters of dyslipedemia(LDL - cholesterol,HDL - cholesterol).Finally, to demonstrat the effect of statin therapy (Atorvastatin 10 - 40 mg) on both novel biomarker and parameters of hyperlipidemia in patients with mild - to moderate degree of calcification with Ca - Score (1 - 399 )ASU.Subjects This study was conducted at the Department of physiol Biochemistry, College of Medicine, University of Baghdad and at the Cardiologic Clinics of Ibn - Al - Bitar Hospital, Baghdad, Iraq, during the period from February 2013 to November 2013. A total of 200 subjects with suspected Coronary Artery Disease (CAD) were encountered, 65 of them not on statin derivatives treatment were included in this study. These patients were investigated firstly for coronary artery calcium by using Multi - Slice Computed Tomography Scanner (Brilliance 64, Philips Medical Systems). The included 65 patients were classified according to their obtained values of coronary artery Ca score into three groups : Group I (GI) included 20 subjects who have coronary artery Ca score=0.0 Agatston Score unit (ASU), aged range (31 - 65 year) and considered as control group, Group II (GII) involved 25 patients with coronary artery Ca score of more than 1 - 399 ASU, aged range (47 - 74 year) and Group III (GIII) included 20 patients who have coronary artery Ca score of more than 400 ASU, aged range (49 - 75 year).Twenty patients of GII were followed after putting them on atorovastatin therapy (10 - 40 mg/day) for 6 - 8 months under supervision of the Consultant Cardiologist. Investigations included serum measurements of Fetuin A, Osteonectin, Visfatin, PTX3 and hs - CRP by using enzyme immunosorbent assay (ELISA) technique. HbA1c, fasting serum glucose and lipid profile parameters were also measured by using spectrophotometric methods. All investigations were performed in patients of the three groups (GI, GII and GIII) and also in patients post statin treatment. Results The results of this study revealed significant reduction in serum levels of visfatin (P=0.005) and osteonectin (P=0.0001) with significant increased of fetuin A in GIII compared with GI. In GII, serum level of osteonectin was significantly decreased, while Fetuin A significantly increased in comparison with those of GI(for both, P=0.0001). There was no significant differences between GII and GIII in the these three parameters.With regard to the changes in serum concentrations of pentraxin III and hs - CRP, the results found that both of these biochemical markers did not differ significantly among the three groups (GI, GII, and GIII).This study also showed that there was significant negative correlation between visfatin and pentraxin III in G I (r= - 0.495, P=0.027), while in GII, significant negative correlation was observed between visfatin and osteonectin (r= - 0.546, P=0.005). Pentraxin III also showed significant negative correlation with BMI in GI (r= - 0.528, P=0.017), significant positive correlation with BMI in GII (r=0.406, P=0.044) and significant positive correlation with with hs - CRP (r=0.482, P=0.031) in G I. In GIII, pentraxin III showed significant negative correlation with ostonectin (r= - 0.489, P=0.029). There is significant negative correlation of osteonectin with glucose in GIII (r=0.566, P=0.009) , In GII and GIII, Fetuin A showed significant negative correlation with BMI values (r= - 0.424, P=0.035)and with serum glucose (r= - .444, P=0.049) . Respectively in G I, hs - CRP concentration was found to be significantly positively correlated with HbA1c (r= - 0.509, P=0.022).The mean value of HbA1c was found to be significantly increased in GIII compared with GI (P=0.03). The serum level of TG was significantly increased in GIII and GII in comparison to G I (p= 0.019).The mean value of serum HDL - C levels of GIII was significantly decreased in comparison with that of GI (P=0.044). The serum level of VLDL is significantly decreased in both GI and GII compared to G III (P=0. 019.The mean value of non - HDL was significantly higher in GIII than in GII and that of GI (P=0. 0289), The mean values of atherogenic index of GIII and GII were significantly higher than in GI along with significant differences between that of GI and that of GII and III(P =0.0008).The present study showed the effect of complete course of statin treatment on the serum levels of novel cardiac biomarkers, the most effected one was serum PTX3 with significant decrease of its serum levels after treatment compared to that before treatment of the same patients group (P=0.0001) combined with significant decrease in serum level of Fetuin A (p=0.027) after complete course of statin treatment. In addition, there was significant increased of serum of HDL in patients after complete course of treatment compared to their concentration before starting treatment (P= 0.0001). Significant correlations were also observed between the novel studied biochemical markers and the degree and number of the major coronary arteries stenosis as well as between Ca score and stenosis.

ازدياد تعبيرالمعلمة الجزيئية miR - 21 التي تتوسط هبوط منتظم لجين PTEN في سرطان الثدي ومصاحبتها مع عوامل التنبؤ المعروفة == MicroRNA - 21 overexpression mediated phosphatase with homology to tensin (PTEN) downregulated in breast cancer in association with clinicopathological status

Author name: شروق محمد عباس التميمي
Supervisor name: سالم رشيد حمودي العبيدي | علي حسين الخفاجي
General topic: Medicine
Specific topic: Tissue Diseases
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: الخلفية : - سرطان الثدي هو الاكثر شيوعا في النساء في كل انحاء العالم . الموروث ( PTEN) هو الموروث المثبط للسرطان ,وهو منظم عكسي(اي مثبط)لاهم طريق لحدوث نمو وتكاثر الخلايا .المعلمة الجزيئية مثل ( miR - 21) هي الجزيئة الاولى التي تم اكتشافها التي تساعد على نمو وتكاثر الخلايا النامية وتمنع موت الخلايا ,اي تطيل من عمر الخلايا النامية. اذا حدث تغير في جزيئة ( miR - 21) وازداد عملها عن الحالة الطبيعية تؤدي الى حدوث نمو مستمر بدون موت الخلايا النامية مما يودي الى حدوث الخلايا السرطانية بالاضافة الى تاثيره على الموروث المثبط لنمو السرطان وهو (PTEN )الذي يعتبر موروث مستهدف له, بحيث يستطيع منع عمل هذا الموروث لذلك يؤدي الى حدوث سرطان الثدي .الهدف من الدراسة : - قياس تعبير المعلمة الجزيئية ( miR - 21) والموروث ((PTEN لمعرفة (زياده او نقصان) على اعتبارهم متغيرات حقيقية عن النسيج الطبيعي وعوامل التنبؤ لسرطان الثدي . المواد وطرائق العمل : - هذه الدراسة هي دراسة تجريبية من الشهر الاول سنة 2013 الى الشهر الاول سنه 2015.تم اخذ خمسين زوجا من العينات, وهذه العينات تؤخذ من سرطان الثدي ومن النسيج الطبيعي للثدي من النساء اللواتي تم استئصال ثديهن بسبب السرطان وهذه العينات تم اخذها في صالة العمليات وكانت العينات طازجة. تم استخلاص شريط ال RNA ثم بعد ذلك تم فحص جزيئة ال (miR - 21) وموروث(PTEN) . وتم تطبيق الواسم المناعي النسيجي الكيمياوي ((IHC لهرمون الاستروجين والبروجستين معher - 2 في العينات التي تم وضعها في بلوكات الشمع وبعد ذلك تم اختيار الحالات الموجبة ل(her - 2)وفحص تضاعف الموروث ( HER - 2) بواسطه تهجين موضعي ذي لونين .النتائج : - معظم الحالات كانت جزيئة ال( miR - 21) مرتفعة في النسيج السرطاني عن ماهو في النسيج الطبيعي وكانت احسن قيمة له (2.940 ) كدليل على ان الجزيئة تغيرت عن قيمته الطبيعية ,وكانت احسن قيمة له (4.156) في حالات انتشار السرطان في العقد اللمفاوية ,وقيمة (6.340 ) كدليل على المراحل المتقدمة من سرطان الثدي .بينما الموروث ( PTEN) كان في جميع الحالات منخفض عن النسيج الطبيعي واحسن قيمة له (0.210) كدليل لتغير تعبيرالموروث عن النسيج الطبيعي , وكانت القيمة) 0.175) دليل انتشاره الى الغدد اللمفاوية, وقيمة (0.098) تمثل المراحل المتقدمة من سرطان الثدي . هنالك علاقه عكسية بين جزيئة ال( miR - 21) والموروث ( PTEN) ولايوجد اي علاقة احصائية بين هذه المتغيرات وبين بقية عوامل التنبؤ الاخرى المعروفة في سرطان الثدي . الاستنتاجات : - التغير في التعبير كل من جزيئة ال ( miR - 21) والموروث (PTEN ) في جميع حالات سرطان الثدي وهنالك علاقة احصائية مع انتشار المرض الى العقد اللمفاوية ومع المراحل المتقدمة من سرطان الثدي . هنالك علاقة احصائية عكسية بين ازدياد التعبير للجزيئة (miR - 21) وانخفاض التعبيرللموروث (PTEN) في عينات سرطان الثدي . | Background : - Breast cancer is the most common cancer in women in worldwide. The phosphatase and tensin homolog gene is a tumor suppressor gene, and a key negatively regulator of cell signaling pathways that regulate growth and survival signaling pathways . More recently, microRNAs are small non protein coding RNAs involved in gene regulation. MicroRNA - 21 was one of the first oncogenic microRNAs and as an anti - apoptotic factor, to be characterized, being up - regulated in numerous tumors including breast cancer. The phosphatase and tensin homolog gene is one of microRNA - 21target genes Aim of the study : - To assess the validity of microRNA - 21 and phosphatase and tensin homolog (PTEN) gene expression as a diagnostic tool for gene alteration .Patients and Methods : - A prospective study , from January 2013 to January 2015. Fifty - pairs of fresh tissues from both breast cancer of invasive ductal carcinoma "NOS" and apparently normal adjacent tissues (from modify radical mastectomy) were by patients were recruited at the Surgical Department /Al - Diawania Teaching Hospital in Al - Diawania city . Total RNA extraction and real - time quantitative polymerize chain reaction technique were used for assessment of microRNA - 21 and phosphatase and tensin homolog gene expression. Tissue sample present in the paraffin embedded blocks belonging to tumor and normal adjacent tissue were used for Immunohistochemistry staining for (estrogen receptor, progesterone receptor and human epidermal growth factor receptor - 2) and dual color - chromogenic insitu hybridization technique for positive human epidermal growth factor receptor - 2 by Immunohistochemistry.Results : - Majority of cases 48(96%) ,were up regulated of microRNA - 21, indicating cancer tissue fold change of microRNA - 21 was significantly higher than that of normal adjacent breast tissue, and the best cutoff value for microRNA - 21 fold change in breast cancer tissues was (2.940)for diagnosis of gene alteration , (≥ 4.156) for positive lymph node involvement and (≥6.340) for higher stage (III,VI).All patients 50(100%) exhibit phosphatase and tensin homolog gene expression down regulation, indicating fold change of cancer tissue for phosphatase and tensin homolog gene expression was significantly lower than that of normal adjacent tissue and the best cutoff value for gene expressional alteration in breast cancer tissues was (0.210) for diagnosis of gene alteration, (≤ 0.175) for positive lymph node involvement and ( ≤ 0.098) for higher stage (III,VI) .Up - regulation of microRNA - A21 and down regulation of phosphatase and tensin homolog gene expression show no significantly correlate with other patients criteria like (age , grade ,size of tumor ,( estrogen receptor, progesterone receptor and human epidermal growth factor receptor - 2) by Immunohistochemical technique and human epidermal growth factor receptor - 2 gene amplification by dual color - chromogenic insitu hybridization technique . Identified significant negative correlation of up - regulation microRNA - 21 and down regulation of phosphatase and tensin homolog gene expression in breast cancer tissues.Conclusion : - gene expression of both microRNA - 21 and phosphatase and tensin homolog was significantly alterated in breast cancer tissues . It has been associated with positive lymph node involvement and higher tumor stage (III,VI)

تاثير التجميد بالتزجيج وطرق الاذابة المختلفة على حيوية وسلامة الحامض النووي للنطف == The Effect Of Laser Thawing After Vitrification On Sperm Motility And DNA Integrity

Author name: شذى صادق المراياتي
Supervisor name: غسان ثابت سعيد
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: ان التجميد بطريقة التزجيج تعتبر من طرق تجميد النطف البشرية وتعتمد على التجميد السريع للماء الى الحالة الزجاجية من خلال زيادة اللزوجة بدون تكوين بلورات داخلية (داخل الخلية). ان التجميد من الممكن ان يؤدي الى تاثيرات ضارة على تركيب ووظيفة النطف البشرية وان الاضرار الناتجة من التجميد من الممكن ان تؤثر على تركيب وسلامة الغشاء البلازمي. وان هذه الاضرار لا تقتصر على اضرار التجميد فقط بل تشمل ايضا على الاضرار الناتجة من عملية الاذابة بسبب اذابة او تبلور الثلج الناتج من التجميد. ان هذه العمليات ( التجميد والاذابة) من الممكن ان تؤثر سلامة الاحماض النووية وكذلك من الممكن ان تؤدي الى تغييرات في نواة النطف البشرية . ان التجميد قد يؤدي الى اجهاد تاكسدي للنطف البشرية كنتيجة الى انحلال الدهون التاكسدي وتقليل الية الدفاعية بواسطة الانزيمات المضادة للاكسدة. خلال عملية التجميد الخلايا والانسجة تخضع الى تغييرات كيميائية وفيزيائية . ان تاثير الليزر ومن خلال التحفيز الضوئي من الممكن ان يقاوم الاضرار الناتجة من التجميد للنطف البشرية وهذا من الممكن ان يؤدي الى تحسين نوعية النطف المجمدة وزيادة قابيلتها على الخصوبة. الهدف : دراسة تاثير الليزر كطريقة لاذابة النطف البشرية المجمدة وتاثيرها على سلامة الاحماض النووية والحركة مقابل الاذابة بدرجة حرارة الغرفة او عن طريق الحمام المائي. الموضوع والطرق : دراسة اجريت على 70 نموذج من النطف البشرية. كل نموذج قسم الى قسمين , قسم قد تم تحضيره بتقنية السباحة الى فوق والقسم الثاني بدون تحضير , وكل قسم قد قسم الى ثلاثة, جمدت, وتم اذابة كل جزء من النماذج بالليزر اوبدرجة حرارة الغرفة او بالحمام المائي بدرجة 37 درجة مئوية . تم دراسة سلامة الاحماض النووية وحركة النطف قبل وبعد التجميد , وكذلك قبل وبعد التحضير بتقنية السباحة الى فوق بطريقة الاكردين اونج (AOT) الحصول بالاعتماد على قياس المذنب (comet).النتائج : اظهرت النتائج ان الاذابة عن طريق الليزر لها تاثير معنوي (P<0.05 ) في تقليل تحطيم الاحماض النووية مع زيادة في الحركة الفعالة للنطف عند المقارنة مع الاذابة بدرجة حرارة الغرفة او بالحمام المائي.الاستنتاج : استنتجت هذه الدراسة ان تجميد الحيامن له اثار مؤذية تؤثر على سلامة الاحماض االنووية وحركة النطف البشرية. ان الاذابة بالليزر تحافظ على سلامة الاحماض النووية والحركة النشطة للنطف وان تقنية السباحة الى فوق كذلك تؤدي الى تنشيط النطف وتقليل تحطم الاحماض النووية. | Vitrification is a method of sperm freezing based on the rapid cooling of water to a glassy state through extreme elevation of viscosity without intracellular ice crystallization. Cryopreservation may lead to deleterious changes of sperm structure and function. Cryoinjury is not limited to the freezing process but may also occur during the thawing process as the ice melts or recrystallizes. It have been reported that cryopreservation/thawing significantly lead to alterations of sperm DNA integrity and could alter the quality of the spermatozoon’s nucleus. Cryopreservation induces oxidative stress in the spermatozoa due to increasing rate of lipid peroxidation and suppression of the antioxidant enzyme defense mechanism. These effects of cryopreservation lead to spermatozoa damage including nucleic acids. Subjects and methods : This prospective study carried on 70 cryopreserved semen samples. Each sample wasdivided into two parts, one part is unprepared and other part was prepared by swim - up technique and each part is divided into 3 parts, freezed and thawed by three methods of thawing by laser irradiation till melting for one part, and by room temperature and water bath at 37°C for the other two parts. The semen parameters were assessed by microscopical examination and the DNA integrity assessed by acridine orange test and comet assay before vitrification and after the three methods of thawing as well as before and after preparation. Result : The results of cryopreserved semen samples showed that laser irradiation thawing has a significant increase in sperm motility as well as a significant decreased DNA fragmentation (P < 0.05) versus room temperature and water bath thawing in addition sperm preparation by swim up method also lead to improve sperm motility and DNA integrity (P < 0.05).Conclusions : Vitrification has a deleterious effect on sperm DNA integrity and motility. Laser irradiation thawing methods of post freezing sperm improves post - thaw motility and DNA integrity. Sperm preparation by swim up technique for sperm preparation increasing motility and decrease DNA damage after thawing . The results of the acridine orange test and comet assay gave relatively similar predictive values for DNA fragmentation.

تاثيرات عقاري المتفورمين والاناستروزول على شكل ووظيفة المبيض في بمتلازمة تكيس المبايض في الجرذان : دراسة شكلية قياسية، نسيجية، وكيميانسيجية == The Effects of Metformin and Anastrozole on the Morphology and Function of the Ovary in Polycystic Ovarian Syndrome Rat Model, Morphometrical, Histological, and Immunoistochemical Study

Author name: علي محسن عبد الامير
Supervisor name: معن حمد الخالصي
General topic: Medicine
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

دراسة الانترلوكين - 6 ,عامل تنخر الورم - الفا وبعض العناصر النذرة في مصل مرضى متلازمة الالم العضلي الليفي == A Study of Serum Interleukin - 6, Tumor Necrosis Factor - ? and Some Trace Elements in Patients with Fibromyalgia Syndrome

Author name: رغد عماد الدين ناجي
Supervisor name: قسمة محمد تركي | محمد هادي العصامي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: متلازمة الالم العضلي الليفي واحدة من اسباب الالم الشائع في الام الجهاز الهيكلي الذي ينتشر في اجزاء مختلفة من الجسم .تتميز هذه المتلازمة بانتشارها في الاناث وهي تصيب الفئات العمرية (۲۰ - ٧۰ ) سنة وتنخفض بعد هذا العمر . ترتبط هذه المتلازمة عادة باعراض متعلقة بالمرض وهي : التعب , تصلب العضلات , اضطراب النوم , ضعف التركيز , اضطرابات نفسية , والصداع .وعلى الرغم من ظهور الكثير من النظريات , الا ان اسباب المرض غير معروفة لحد الان .الهدف : لقياس مستوى تركيز الانترلوكين - ٦ , عامل تنخر الورم - الفا وعلاقتها باعراض الالم وقباس مستوى بض العناصر الندرة ( الزنك , النحاس , السيلينيوم ) لمرضى هذه المتلازمة.المكان : اجريت هذه الدراسة للفترة الزمنية من تشرين الثاني ۲۰١۰ الى تموز۲۰١١. وتم اختيار المرضى من المراجعين للعيادة الاستشارية الخارجية في مستشفى بغداد التعليمي لقسم امراض المفاصل والتاهيل . تم اجراء التحاليل في المختبرات التعليمية العائدة لمستشفى بغداد التعليمي ومركز السموم في مستشفى الجراحات التخصصي .الاشخاص : تضمنت الدراسة ٥٧ مريضا من مرضى متلازمة الالم العضلي الليفي ( ٤٩ انثى + ٨ ذكور ) تراوحت اعمارهم بين ( ٤٣٫۰١٧ ± ١۰٫٩٤٥ ) سنة ومجموعة المتبرعين الاصحاء عددهم ٣٤ (۲٨ انثى + ٦ ذكور ) .طرق العمل : تم قياس كل من الانترلوكين - ٦ , عامل تنخر الورم - الفا , البروتين التفاعلي - سي وهرمونات الغدة الدرقية باستخدام طريقة اليزا .اما العناصر الندرة ( الزنك , النحاس , السيلينيوم ) فقد تم استخدام طريقة الامتصاص الطيفي الحراري .تم التعبير عن النتائج بالمعدل ± معدل الغلط القياسي ومع احتمالية وقوع الغلط اقل من ( ۰٫۰٥ ) كمغزى احصائي .النتائج : اظهرت النتائج ان هناك فروقا معنوية بين مصل الانترلوكين - ٦ , عامل تنخر الورم - الفا , والبروتين التفاعلي - سي - عالي الحساسية , بين مرضى متلازمة الالم العضلي الليفي ومجموعة المتبرعين الاصحاء .اما تحاليل هرمونات الغدة الدرقية , فلم تكن هناك فروقات معنوية بين المرضى ومجموعة الاصحاء .وكانت هناك فروقات معنوية في نتائج العناصر الندرة ( الزنك , النحاس , السيلينيوم ) بين المرضى والاصحاء .الاستنتاج : البحوث السلوكية البايوكيميائية ركزت على فهم العلاقة بين متلازمة الالم العضلي الليفي والسايتوكينات , وهذا قد يقود الى فهم اكثر لاعراض هذه المتلازمة . ان ارتفاع مستوى الانترلوكين - ٦ , وعامل تنخر الورم - الفا لمصل مرضى المتلازمة يعد محفزا لاعراض التعب , زيادة الشعور بالالم , الكابة , وعامل التنخر واظهر ايضا ارتباطا بالتوتر العصبي واضطراب النوم .الميكانيكية المقترحة هي ان الانترلوكين - ٦ يحفز افرازات هرمونات قشرة الكظر المعتقد تتحفز بافراز هرمون الافراج عن الكورتيكوتروبين الفرز من قبل تحت المهاد. | Background : Fibromyalgia Syndrome (FMS) is a common chronic widespread pain syndrome , usually associated with other somatic and psychological symptoms including fatigue , sleep disturbance , and cognitive difficulties like difficult concentration . The prevalence of fibromyalgia is reported to be 2 - 4% with a female to male ratio of about 9 : 1, its prevalence increase with age from (30 - 50) , declining thereafter . The etiology and pathophysiology of fibromyalgia has not been clearly understood and that makes the disorder a frustrating condition for the patients and the physician . The etiopathology of fibromyalgia is not clear , though cytokines like interleukin - 6 is a potent stimulator of hypothalamic - pituitary axis via activation of the hypothalamic corticotrophin - releasing hormone (CRH) . Objective : To evaluate interleukin - 6 (IL - 6), tumor necrosis factor - alpha (TNF - α)and high sensitivity - C Reactive Protein (hs - CRP) and their relation with symptoms included in the underlying etiopathology of fibromyalgia patients since interleukin - 6 is a potent stimulator of hypothalamic pituitary axis via activation of the hypothalamic CRH .Setting : This cross sectional case - control study was performed at the Rheumatology and Rehabilitation Consultation unit , Baghdad Teaching hospital/medical City during the period from November 2010 to July 2011 . The laboratory tests were done in The Teaching Laboratory / Medical City , and The Toxicology Center / The Hospital of Specialized Surgeries Subjects : The study included 57 patients with FMS ( 49 females + 8 males ) , their mean age ( 43 ± 10.9) years , and 34 healthy control individuals ( 28 females + 6 males ) who their age and sex matching with the FMS patients . Methods : Serum levels of Interleukin - 6 , Tumor Necrosis Factor - α , high sensitivity - C Reactive Protein , and thyroid hormones were estimated by using ELISA technique . Zinc(Zn) , Copper(Cu) , and Selenium(Se) were estimated by Flame , and Flameless Atomic Absorption Spectrophotometric techniques. Results : The results show that the mean (± S.D.) values of serum IL - 6 , TNF - α , and hs - CRP concentrations were significantly higher in fibromyalgia patients than healthy control (p<0.05). Mean values of Sera of thyroid hormone levels were non - significant when compared with control group . The results of serum concentration of Zn , Cu , and Se of FMS patients were significantly different from that of control group ( p<0.05) .Conclusion : The elevated IL - 6 and TNF - α in FMS patients considered a promotion to fatigue , hyperalgesia , pain and depression . TNF - α is also associated with stress , rapid eye movement sleep and allodynia .

الندرونيت الصوديوم في الفصال العظمي للمفصل الظنبوبي : تاثيره على معلمات بناء وانحلال الغضروف, لبتين والفعاليات الفيزيائية للمرضى العراقيين == ALENDRONATE SODIUM IN KNEE OSTEOARTHRITIS : EFFECTS ON ANABOLIC AND CARTILAGE DEGRADATIVE MARKERS, CIRCULATING LEPTIN AND THE CLINICAL ACTIVITY IN IRAQI PATIENTS

Author name: سيناء عبد الامير كاظم
Supervisor name: حيدر مهدي جواد | سامي سلمان شهاب
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: المقدمة : الفصال العظمي من الامراض الاكثر شيوعا في العالم والذي يصيب النساء اكثر من الرجال ويتميز هذا المرض بانه يحدث نتيجة لخلل في جميع مكونات المفصل متمثلا بانحلال الغضروف وتهشم العظم الملاصق له والتهاب الغلاف المحيط بالمفصل. متسببا في تقليل عرض الحيز الانسي للمفصل.الهدف من الدراسة : تقييم دور عقار الندرونيت الصوديوم ALN)) لمرضى الفصال العظمي للمفصل الظنبوبي ومعرفة تاثيره على مستوى الفعاليات الحياتية للمرضى التي تتمثل بمستوى الالم ومستوى تيبس المفصل وتاثيره على مستوى وظيفة المفصل الظنبوبي. وتقييم مدى تاثيره على معلم البناء 1β TGF , معلم انحلال الغضروف CTXII, مستوى لبتين في الدم, مايلوبيروكسيدييز MPO, الكالسيوم, مستويات الدهون بانواعه وفوسفات الالكلاين والبروتين التفاعلي C.طريقة العمل : ادخل 116 مريضا مصابا بمرض الفصال العظمي للمفصل الظنبوبي ضمن هذه الدراسة وكانوا بعمر من 45 سنة او اكثر وبالدرجة الثانية من المؤشر الشعاعي لكلكرين ولورنس (KL) مع قياس الوزن والطول, عرض الحيز الانسي للمفصل الظنبوبي, التغييرات الكيمياوية المختبرية المتمثلة بمستوى الكالسيوم, مستويات الدهون بانواعه متمثلة بقياس نسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنيه عالية الكثافة والبروتينات الدهنية واطئة الكثافة , فوسفات الالكلاين والبروتين التفاعلي C وقياس المعلمات المناعية بطريقة الالايزا لكل من معلم البناء 1β TGF , معلم انحلال الغضروف CTXII, مستوى لبتين في الدم, مايلوبيروكسيدييز MPO. حيث تم قياس المستويات المذكوره اعلاه قبل البدا باخذ العلاج. تم اعطاء المرضى 10 ملغم من عقار الندرونيت الصوديوم (ALN) واعيد تقييم حالة المرضى بعد اكمال الاشهر الثلاثة من العلاج.النتائج : لوحظ ان هناك تحسن معتد على الفعاليات الفيزيائية واعراض الفصال العظمي , مع انخفاض معتد في معلم TGF β 1 (لاتوجد مقارنة بين تغيره وتحسن حالة المرضى, غير ان له علاقة بزيادة كتلة الجسم) , معلم انحلال الغضروف ) CTXIIحيث له علاقة بتحسن الفعاليات الحياتيه للمرضى), مستوى لبتين في الدم. مع تغير غير معتد في عرض الحيز الانسي للمفصل, مستوى الكالسيوم, مايلوبيروكسيدييز, مستويات الدهون بانواعه متمثلة بنسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنيه عالية الكثافة والبروتينات الدهنية واطئة الكثافة , فوسفات الالكلاين والبروتين التفاعلي C. الاستنتاج : عقارالندرونيت صوديوم له فعالية كبيرة في تحسين الفعاليات الحياتية لمرضى الفصال العظمي وتقليل الالم الناتج من المرض دون تاثير معتد على تغير تركيب المفصل على الرغم من تقليله لمعلم اضمحلال الغضروف. عدم تاثير العقار على مستويات الدهون في الجسم المتمثلة بنسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنية عالية الكثافة والبروتينات الدهنية واطئة الكثافة يجعله امنا نسبيا للمرضى المصابين باختلال نسبة الدهون. | Background : Osteoarthritis is a common arthritic disorder and responsible for 2 % of disability of people in all world. Osteoarthritis has believed to be principally a cartilage defect associated with focal articular cartilage degradation. Alendronate sodium (ALN) interferes with normal bone formation and turnover cycle in the body by slowing bone loss with elevating bone mass. . Aims of the study : This study was done to evaluate the effects of alendronate sodium on disease activity and physical function in patients with knee osteoarthritis, to evaluate the biochemical parameters in osteoarthritis patients including serum alkaline phosphate, calcium, c - reactive protein and lipid profile in form of analysis of serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein. Also to study the impact of ALN on anabolic in form analysis of serum transforming growth factor beta 1 (TGF - β1) , cartilage degradative in form of C - terminal cross linked - telopeptide of type II collagen (CTXII), Myloperoxidase (MPO) and leptin markers in osteoarthritis patients and to determine its effectiveness in slowing progression of disease. .Patients and methods : The patients were habitants of the cities of Al - Diwaniyhia (consulting the Rheumatologic Unit in Al - Diwanyhia Teaching Hospital) and Baghdad (consulting the Rheumatologic Unit in Baghdad Teaching Hospital). From those patients, 116 osteoarthritic patients 45 years old and more with Kellgren and Lawrence X - ray grade II and more with knee pain were enrolled in this study. Base line assessment was done including measurement of joint space width, assessment of WOMAC scoring(pain, function and stiffness), body mass index and the biochemical parameters (serum alkaline phosphate, calcium, c - reactive protein and lipid profile in form of analysis of serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein) with enzyme - linked immunosorbent assay (ELISA) analysis of serum (transforming growth factor beta 1 (TGF - β1) , C - terminal cross linked - telopeptide of type II collagen (CTXII), MPO and Leptin. They were instructed to take ALN 10 mg daily. Reassessment was done after 3 months of treatment. Results : A significant symptomatic improvement in WOMAC scoring regarding pain and stiffness (P<0.001) were observed associated with non significant improvement in function, significant reduction (P<0.001) in serum CTXII, Leptin and TGF - β1. A nonsignificant reduction in serum calcium and MPO, associated with no significant changes in serum Alkaline phosphatase, C - reactive protein, lipid profile(serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein) and joint space width were also reported. CTX II after treatment showed no significant correlation with BMI, however it showed significant positive correlations with WOMAC score. On the other hand, TGF - β1 showed significant positive correlation with BMI, but no significant correlations with WOMAC score. Conclusion : Alendronate sodium in patients with osteoarthritis has clinical efficacy in reducing symptoms especially pain through inhibition of leptin and CTXII with no significant structural improvement despite reduction of CTXII and TGF - β1 with no effects on inhibition of further disease progression. Relatively safe in old patients with dyslipidemia since there is no associated lipid disturbances with ALN. The reduction in level of CTX - II in the present study may be considered as one of the mode of actions of alendronate in patients with osteoarthritis

تقييم مستويات فيتامين د في مصل الدم وتغاير الجين المشفر لمستقبلات فيتامين د لدى النساء المصابات بمتلازمة تكيس المبايض == Assessment of SerumVitamin D Levels and Vitamin D Receptor Gene Polymorphism in Women with Polycystic Ovary Syndrome

Author name: رنا علي حمدي
Supervisor name: زينا حسن عبد القهار
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Polycystic ovary syndrome is a common endocrinopathy in women of reproductive age with primary manifestations of infertility, menstrual irregularity, and clinical or biochemical evidence of hyperandrogenism.Insulin resistance is predominant in women with this disorder independently of obesity and is contributed to reproductive and metabolic defect seen in this syndrome.Vitamin D was assumed to have a physiological effect in reproduction through binding to nuclear receptors which have been distributed in the uterus, oviduct, ovary, placenta, and fetal membranes. Besides, genes included in vitamin D metabolism have been assumed as candidate genes for the polycystic ovary syndrome susceptibility. Vitamin D receptor gene polymorphisms were suggested to play an influential role on insulin secretion and sensitivity in women with this syndrome.Objective : This study was planned to evaluate serum 25 - hydroxy vitamin D3 levels in women with polycystic ovary syndrome and compare their levels with age and body mass index matched healthy controls. Also, assess the correlation between insulin resistance and 25 - hydroxy vitamin D3 among women with this syndrome. In addition, investigate the possible association between Cdx2 (G/A) single nucleotide polymorphism of vitamin D receptor gene and the risk of polycystic ovary syndrome.Materials and Methods : This case - control study involved eighty eight (88) women from 18 to 34 years of age. Women were attended to Infertility Center in Baghdad Teaching Hospital during the period from March 2017 to June 2017. Women were divided into two groups : group 1 - consisted of forty five (45) newly diagnosed women with polycystic ovary syndrome and group 2 - consisted of forty three (43) healthy women (as controls). Women with polycystic ovary syndrome were diagnosed according to Rotterdam criteria when two out of three following criteria are found, these include oligoovulation and/orIIIanovulation, clinical and/or biochemical hyperandrogenism and polycystic ovaries as defined by ultrasonography.Each serum sample was analyzed for measuring 25 - hydroxy vitamin D3, luteinizing hormone, follicle - stimulating hormone, free testosterone, and insulin by enzyme linked immunosorbent assay. While serum calcium and fasting serum glucose were measured by spectrophotometer. Moreover, DNA samples were amplified and analyzed for the Cdx2 polymorphism of vitamin D receptor gene using allele specific multiple - polymerase chain reaction.Results : Significantly lower levels and higher prevalence of vitamin D deficiency were found in women with polycystic ovary syndrome compared to age and body mass index matched healthy controls (p=0.0001). Also, significant negative correlations were found between serum 25 - hydroxy vitamin D3 levels and fasting serum glucose (r= - 0.484, p=0.01), insulin (r= - 0.422, p=0.04) and HOMA - IR (r= - 0.542, p=0.0001) in patients group. Besides, no significant difference in genotypic distribution of Cdx2 polymorphism of vitamin D receptor gene between patients and controls. GG carriers (p=0.141), GA carriers (p=0.189), and AA carriers (p=1). However, the results found significantly higher serum levels of luteinizing hormone (p=0.002) and luteinizing hormone/follicle - stimulating hormone ratio (p=0.003) in GG carriers than GA and AA carriers for patients group and lower levels of serum 25 - hydroxy vitamin D3 in GG carriers than GA and AA carriers for both patients (p=0.00001) and controls (p=0.00001).Conclusion : Cdx2 polymorphism of vitamin D receptor gene has an association with severity of clinical features seen in polycystic ovary syndrome, but not with risk of development of the disease meaning that genetic variation are not directly linked to risk of this syndrome but may indirectly affect disease development via regulation of vitamin D and/or calcium levels.

الدراسة الكهروفسلجية وحالة الاجهاد التاكسدي في مرض تصلب الاعصاب المنتشر == NEUROPHYSIOLOGICAL STUDY AND OXIDATIVE STATUS IN MULTIPLE SCLEROSIS

Author name: عمار احمــد ثامـــر
General topic: Medicine
Specific topic: Neurophysiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • قسم الفسلجة السريرية
First pages:
Abstract: تصلب الاعصاب المنتشر مرض التهابي مزمن مجهول السبب، يتصف بفقدان الميالين (النخاعين) مع الميل الى اصابة محور الليف العصبي، ويصيب الاشخاص في الاعمار المتوسطة من حياتهم مع ارجحية اصابة النساء اكثر من الرجال.اشتملت الدراسة على فحوص الكهروفسلجية والكيمياء الحيوية بالاضافة الى فحوص شعاعية، لمئة واثني عشر مريضا مشخصا سريريا وفقا لمعايير مكدونالد (McDonald's criteria) وقورنت نتائجهم مع مجموعة ضابطة تضم خمسين شخصا متطوعا ومعافي.احتوت الفحوص الكهروفسلجية دراسة كامن العصب البصري المثار، في حين اشتملت الفحوص الكيميائيحيوية على قياس تراكيز النحاس ، الزنك ، المغنيسيوم ونسبة الزنك/النحاس في مصل الدم بالاضافة الى الفحوص الشعاعية بواسطة جهاز الرنين المغناطيسي.اشارت النتائج الى وجود زيادة معنوية في متوسط كمون الموجه الموجبة (P100) ولمتوسط فرق الكمون بين العينين مع نقصان معنوي لمتوسط مدى المركب (N75 - P100) لمرضى تصلب الاعصاب المنتشر، في حين لم يوجد فرق معنوي في متوسط فرق المدى للمركب (N75 - P100) بين العينين عندما قورنت بقيم المجوعة الضابطة. وكان الاكثر شذوذا في فحص كامن العصب البصري المثار لمرضى تصلب الاعصاب المنتشر هو الاطالة في كمون الموجة الموجبة (P100) ثم الاطالة في فرق الكمون بين العينين ومن ثم التغير في شكل الموجة ثم الاختزال في متوسط فرق المدى المركب (N75 - P100)، في حين كان الاختزال في متوسط مدى المركب (N75 - P100) الاقل تغيرا. وذلك يرجح التاثير الرئيسي لازالة الميالين على التوصيل والمتمثل كهروفسلجيا بزيادة الكمون.كانت هناك علاقة خطية موجبة بين نسبة العجز السريري للمرضى حسب مقياس كرتزك (Kurtzke) مع كمون الموجة الموجبة (P100) بينما كانت العلاقة الخطية سالبة مع مدى المركب (N75 - P100). وهذا يعني ان زيادة معدل العجز للمرضى يترافق بزيادة في عمليات ازالة الميالين مع الميل الى اصابة محور العصب.اظهرت دراسة الفحص الشعاعي ان جميع المرضى كانت لديهم لطخات في جهازهم العصبي المركزي حسب الفحص بالنموذج الثاني، وان توزيع اللطخات لا يتاثر مع العمر او نوع مرض تصلب الاعصاب المنتشر. بالاضافة لذلك كانت هناك علاقة خطية موجبة بين العدد الكلي للطخات مع نسبة العجز السريري، كذلك زيادة نسبة العجز السريري لدى المرضى المصابين بنوع الازدياد الاولي والثانوي عن غيرهم من الانواع، اضافة الى زيادة عدد اللطخات عند المرضى المصابين بنوع الازدياد الاولي والثاني على غيرهم من انواع المرضى.اظهرت الدراسة زيادة معنوية في تركيز النحاس ونقصان معنوي في تركيز الزنك والمغنيسيوم ونسبة الزنك/النحاس في مصل الدم عند المرضى، مما يشير لوجود عملية اجهاد تاكسدي لدى مرضى تصلب الاعصاب المنتشر.بالاضافة لذلك وجد ان هناك علاقة خطية موجبة بين نسبة العجز السريري للمرضى حسب مقياس كرتزك (Kurtzke) مع تركيز النحاس بينما كانت العلاقة الخطية سالبة مع تركيز كل من الزنك والمغنيسيوم ونسبة الزنك/النحاس في مصل الدم عند المرضى.يستنتج من هذه الدراسة ما يلي : 1. ان فحص كامن العصب البصري المثار يعكس انحدار في وظيفة المجال الكلي للنظر ويؤيد الدراسات السابقة في كشف الافات غير الظاهرة سريريا.2. امكانية جعل عجز مرضى تصلب الاعصاب ان يكون بشكل محسا وموضوعيا عن طريق هذا الفحص الكهروفسلجي.3. ان فحص الرنين المغناطيسي يستطيع ان يكشف اللطخات اكثر في نوع الازدياد الاولي والثانوي اكثر من بقية انواع مرض تصلب الاعصاب المنتشر.4. ان نسبة العجز السريري لدى مرضى تصلب الاعصاب المنتشر بنوعيه الازدياد الاولي والثانوي هي اكثر من بقية الانواع.5. كلما ازداد عدد اللطخات في الجهاز العصبي المركزي للمرضى كلمزاادت نسبة العجز السريري.6. وجود اختلال في التوازن الطبيعي بين الجذور الحرة ومضادات الاكسدة مع الميل باتجاه زيادة الاجهاد التاكسدي لدى مرضى تصلب الاعصاب المنتشر.7. امكانية الوصول الى تقييم مستوى العجز الحركي للمرضى بطرق كيميائيحيوية مثل قياس تركيز النحاس، الزنك والمغنيسيوم في مصل الدم.8. التغيرات الحاصلة نحو الاجهاد التاكسدي واختلاف االعناصر الزهيدة قد يكون السبب للمرض، او مرحلة من مراحل الامراض او نتيجة للمرض، وبكل الحالات يظهر انه يلعب دورا فعالا في هذا المرض ويحتاج الى المزيد من الدراسات المستقبلية. | Multiple sclerosis (MS) is an idiopathic chronic inflammatory disease characterized by demyelination that can be associated with axonal degeneration and is the leading cause of a non - traumatic neurological disability in young adults, with female affection more than male. This study was designated to assess the (1) changes in Visual Evoked Potential (VEP), (2) their frequency in MS patients with and without visual symptoms, (3) evaluate the oxidative status and trace elements in patients with MS, (4) correlate the results of VEP and biochemical studies with the disability of MS patients, in addition to assessing the Magnetic Resonance Imaging (MRI) findings in these patients and their changes with the disability status of patients with MS.Electrophysiological and biochemical studies in addition to imaging examination, had been carried out on 112 patients (35 males and 77 females) who were assessed clinically having definite MS, and fulfilled McDonald's Committee criteria and 50 normal control subjects (17 males and 33 females).Electrophysiological study involved visual evoked potential (VEP) of both eyes, in which P100 latency and P100 interocular (IO) latencies with amplitudes of N75 - P100 complex in addition to P100 interocular amplitude were examined, while Biochemical assay involved the evaluation of the oxidative stress by measuring the concentration of serum Copper, Zinc and Magnesium, in addition to the zinc/copper ratio measurement.This study reported statistically significant increase in the P100 latency and interocular latency difference, and a significant decrease in the N75 - P100 complex amplitude, while there was no significant difference in the IO amplitude difference between MS patients and control.The changes in the P100 latencies were more frequently recorded then the IO latency difference, while IO amplitude difference, N75 - P100 complex amplitude and the wave - form of potentials were the least, which is due to the demyelination effects on nerve conduction, and collectively, VEP abnormality was present in (79.46 %) of the examined MS patients.The changes in the VEP parameters recorded in MS patients who have clinical visual presentations, and the changes were also present in those patients without visual clinical picture, which indicates the presence of subclinical lesions in the visual pathways, this fact makes VEP test as an important diagnostic aid in MS patients.Moreover, the results of this study present a positive correlation between the P100 latencies with the patients disability (EDSS score), while with a negative correlation between the amplitudes of VEP potentials with the same score of MS disability.Regarding, neuroimaging, the distribution of MS lesions in the CNS is neither correlated with the type of MS nor the age of patients. While, the patients disability is increased with the increase of total number of MRI lesions. However, patients disability is more with the progressive forms of MS in which more number of MRI lesions in their CNS than other forms of MS disease are detected.Biochemically, the study for oxidative stress reveal a highly significant increase in the serum copper, and a significant decrease in the serum zinc, magnesium and zinc/copper ratio levels.The association between different clinical presentations and biochemical findings was not evident, however, there was a positive correlation between the clinical disability score (EDSS) with the serum concentration of Copper, and a negative correlation with that of Zinc, Magnesium and Zinc/Copper ratio.In conclusion, this study shows : 1 - VEP study can reveal a suspected deterioration of visual function and objectivate unsuspected clinically silent lesions.2 - Disability and physical deterioration of MS patients can be quantified and clarified by using this electrophysiological test. 3 - It manifests that MRI reveals CNS lesions in progressive forms more than other forms of MS.4 - Patients with the progressive forms associate with more disability.5 - The patients disability correlates with the total number of MRI lesion in MS patients.6 - It suggested the existence of a clear imbalance in oxidant - antioxidant status, towards an increasing oxidative state in MS patients.7 - Physical disability of MS patients can also be quantified by biochemical studies and serum Copper, Zinc, Magnesium and Zinc/Copper ratio levels can be used as biological markers of patients disability. 8 - The changes in the oxidative state and trace elements metabolism in MS, may be the cause behind the disease, a stage during its pathogenesis, or as a results of it, in all states they seems to have a potential role in this disease and need further studies to clarify it.

تقييم الطفرات الوراثية ل BRAF, IDH1 وفقدان 1p/19q في الاورام الدبقية للمرضى القراقيين : دراسة نسيجية مناعية كيميائية مع التهجين الموضعي المتفلور == Assessment of BRAF, IDH1 mutations and 1p/19q loss in Gliomas in Iraqi patients. Immunohistochemical and Fluorescence in Situ Hybridization study

Author name: زهراء مروان شعبان العمر
Supervisor name: سالم رشيد حمودي العبيدي
General topic: Medicine
Specific topic: Diseases - Genetics
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Background : - Gliomas account for almost 80% of primary malignant brain tumors, so, they considered the most common primary malignant brain tumors in adults. The major types of glial tumors are astrocytomas, oligodendrogliomas, and ependymomas. Isocitrate dehydrogenases 1, BRAFV600E and 1p/19q co - deletion are important molecular markers nowadays for diagnosis and prognosis of gliomas. Isocitrate dehydrogenase mutation might be encounter in the low - grade glioma, occurs in early stages of development and directs the progression of the tumor to a higher grade. BRAFV600E is a point mutation, resulting in a valine to glutamic acid substitution at position 600 and this mutation occurs more in pediatric gliomas but less frequent in adult gliomas. Co - deletion of 1p/19q results from a non - balanced translocation t (1 : 19) (q10 : p10) with subsequent loss of one of the derivative chromosomes and highly associated with oligodendroglial tumors morphology and improved survival.Aim of the study : - To assess the frequency of Isocitrate dehydrogenases 1 and BRAF mutations in Iraqi patients with gliomas by immunohistochemical study, to correlate their immunoreactivity with some clinicopathological parameters. To validate frequency of 1p/19q loss by Fluorescence in Situ Hybridization study and correlate the deletion with the some parameters.Patients and Methods : Formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in Baghdad city were collected in this retrospective selective study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that is manual tissue microarray, in which twenty - four small cores (each measure 2mm) of represented tissue made, then cut by microtome. IHC detection of Isocitrate dehydrogenases 1 and BRAFV600E antibodies did by Dako autostainer link 48. Assessment of co - deletion of 1p/19qAbstractIII

الببتيدات النيتروجينية الدماغية الابتدائية نوع (NT - PROPBNP) ومؤشرات كيميائية حيوية اخرى في المصل كدلائل لضرر العضلة القلبية لدى مرضى المتلازمة التاجية الحادة == SerumN - Terminal Pro Brain Natriuretic Peptide and Some Other Biochemical marker as Indicators of MyoCardial Damage in patients with Acute Coronary Syndrome

Author name: وفاء منصور ميرزا
Supervisor name: احسان خليل عباس | باسل نجيب سعيد
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: المتلازمة التاجية الحادة تشير الى اية مجموعة من الاعراض تعزى الى اعاقة الشرايين التاجية. العلامة الاكثرشيوعا التي تحفز تشخيصACS هى الم الصدر، في اغلب الاحيانا تشع من الذراع الايسراو زاوية الفك، تشبه الضغط في النوعية، ومصحوبة بجيشان النفس والتعرق. متلازمة التاجية الحادة تحدث عادة كنتيجة لاحدى المشاكل الثلاث : الاحتشاء القلبي المرتفع ال ST ،الاحتشاء القلبي غير المرتفع الST ،اونوبة قلبيةغيرمستقرة. تسمى هذه الانواع حسب مظهر تخطيط القلب الكهربائي والمؤشرات الحيوية مثل كرياتين كاينيس، تروبونين خاص بالقلب ووالببتيدات النتروجينية الدماغية نوع BNPوبادرته (NT - proBNP) تفرز من قبل اذين وبطين العضلة القلبية للانسان. الارتفاع في مستوى الببتيدات النتروجينية نوع BNPوبادرته (NT - proBNP) بعد الاحتشاء القلبي المرتفع ال ST يربط بحجم الاحتشاء وتشوهات حركة جدار القلب.يبقى تخطيط صدى القلب الذي يستعمل بشكل متكرر وعادة الاختبارالتصويرى الاولي لتقييم كل امراض الاوعية القلبية المتعلقة بوظيفية،هيكلية اوشذوذ حركة الدم للقلب اوالشرايين الكبيرة.الهدف من الدراسة : 1 - لتحديد فائدة الببتيدات النتروجينية الدماغية ,التروبونين العالي الحساسية والبروتين التفاعلي العالي الحساسية كدلائل كيميائية في التشخيص المبكر لنقص التروية الدموية القلبية. - ربط مستوى الببتيدات النيتروجينية الدماغية الابتدائية مع معاملات تصوير صدى القلب.2في الحد من تاثير نقص التروية الدموية القلبية. تقييم كفاءة محلل الخثرة الليفية - 3 في نقص التروية الدموية القلبية. لربط العلامات الكيمياوية الحيوية المدروسة بعامل خطر - 4والطرق : المواضيع تم تضمين سبعين من المرضى الذين يعانون من انواع مختلفة من متلازمة الشريان التاجي الحادة وعشرين من الافراد الاصحاء في الدراسة. تم تقسيم المرضى الى خمس مجموعات : 1 - الاحتشاء القلبى واسعة النطاق : وشملت هذه المجموعة 26 (37.14٪) مريض. 2 - الاحتشاء القلبى في الحاجز الامامي : وشملت هذه المجموعة 10 (14.29٪) مريض. 3 - الاحتشاء القلبى الامامي : وشملت هذه المجموعة 2 (2.86٪) مريض. 4 - الاحتشاء القلبى السفلي : وشملت هذه المجموعة (25.71٪) مريض. 5 - الذبحة الصدرية غير المستقرة : وشملت هذه المجموعة 14 (20.00٪) مريض المرضى عادة عولجوا في وحدة القلب المخصصة لان هذا يوفر وسيلة مريحة لتركيز الخبرات والانعاش اللازم.تمت دراسة عوامل الخطر التي تزيد من حدوث متلازمة الشريان التاجي الحادة والتي شملت ارتفاع ضغط الدم، داء السكري، والتدخين، والسمنة، وتاريخ من امراض نقص تروية القلب. | Acute Coronary Syndrome (ACS) refers to any group of acute symptoms and sign, attributed to sever obstruction of the coronary arteries . Acute coronary syndrome usually occurs as a result of one of three problems : ST elevation myocardial infarction, non ST elevation myocardial infarction, and unstable angina. These types are named according to the results of the electrocardiogram (ECG/EKG) and cardiac biomarkers like Creatine Kinase, Cardiac - Specific Troponins and Natriuretic Peptides. B - type natriuretic peptide (BNP) and its precursor, N - terminal pro - BNP (NT - proBNP), are secreted by human atrial and ven¬tricular myocardium. The rise in BNP and NT - proBNP levels after STEMI correlates with infarct size and regional wall motion abnormalities. Echocardiography remains the most frequently used and usually the initial imaging test to evaluate all cardiovascular diseases related to a structural, functional, or hemodynamic abnormality of the heart or great vessels. The aim of the study : 1 - To determine the usefulness of NT - PRO Brain Natriuretic Peptide ,hs - troponin I and hs C - reactive protein as a biochemical indicator in early diagnosis of ischemia.2 - To correlate NT - PRO Brain Natriuretic Peptide level with echo cardio parameters.3 - To evaluate the efficiency of fibrinolytic drug (Actilyse) in reducing ischemia effect.4 - To correlate the studied biochemical markers with the risk factor of cardiac ischemia. Subjects and Methods : Seventy patients with acute coronary syndrome and twenty healthy individuals are included in the present study. Patients were classified into five groups according to their type of ischemia : I - Extensive myocardial infarction : this group included 26 (37.14%) patients.II - Anteroseptal myocardial infarction : this group included 10 (14.29%) patients.III - Anterior myocardial infarction this group included 2(2.86%) patients. IV - Inferior myocardial infarction this group included 18 (25.71%) patients. V - Unstable angina : this group included 14 (20.00%) patients. Patients are usually managed in a dedicated cardiac unit because this offers a convenient way of concentrating the necessary expertise, monitoring and resuscitation facilities.The risk factors that increased the incidence of acute coronary syndrome were studied which included hypertension, diabetes mellitus, smoking, obesity and history of ischemic heart disease.

الكشف الجزيئي عند مرضى HLA - B27 والمصلي لـ التهاب الفقار المقسط مع بعض الارتباطات السريريه == Molecular and Serologic Detection of HLA - B27 Among Ankylosing Spondylitis Patients With Some Clinical Correlations

Author name: ميادة محمد علي عبد الهادي الضايف
Supervisor name: بسام موسى صادق الموسوي
General topic: Medicine
Specific topic: Diseases - Genetics
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: مرض التهاب الفقار المقسط (AS)هو مرض التهابي مزمن يصيب المفاصل العجزية الحرقفية، العمود الفقري وليس من النادر المفاصل المحيطيه، كما يؤثر على 0.1 - 1% من عامة الناس. السبب الدقيق لـ AS غير معروف لكن تم تحديد استعداد وراثي قوي لـ AS مع ارتباط قوي بـ HLA - B27 الايجابيه، HLA - B27 يوجد في > 90% من المرضى AS البيض و50 - 80% من المرضى AS غير البيض. هناك عدة طرق للكشف عن HLA - B27 كالطرق المصلية (طريقة مقايسة الممتز المناعي المرتبط بالانزيم(ELISA) وطريقة اختبار السمية للخلايا اللمفاوية الصغيرة(MLCT) والتقنيات الجزيئية (القائمة على الحمض النووي مثل PCR).كانت الطرق المصلية في البداية هي الاختبارات القياسية للكشف عن المتسضدات HLA - B27 ولكن استبدلت بالطرق الجزيئيه على اساس الحمض النووي لان الطرق المصلية لها عيوب عديدة كان تكون حساسة للتنظيم السفلي اوالتغييرات التوافقية للمستضدات، لا يمكنها اكتشاف فروق بنية البروتين الناتجة عن تعدد اشكال النوكليوتيدات الاحادي او المحدود ، لها نتائج سلبيه كاذبة او ايجابية كاذبة لعدة اسباب مثل التفاعل المتصالب للاجسام المضادة مع مستضدات فئة HLA class I المختلفة بسبب التشابه الواسع النطاق داخل الطبقه، وتغيير الحواتم المستضدي، تلوث الصفائح الدموية او كريات الدم الحمراء. تحدث هذه التغييرات في بنية البروتين (المستضد) في كثير من الاحيان استجابة للعدوى المتزامنة، والعلاج بالعقاقير والحالة الصحية للمريض.لاتتاثر التقنيات القائمة على تفاعل البلمرة المتسلسل (PCR) بهذه التغييرات في بنية البروتين، وبالتالي فهي اكثر تمثيلا لحالة منتج الجين (الحاضر/ الغائب)؛ بالاضافة الى انها قادرة على اكتشاف اختلاف اساسي واحد في تسلسل DNA بين الاليلين، ويمكن تطبيقه على اي مصدر من الحمض النووي الجيد بغض النظر عن قابلية الخليه. الهدف من الد راسة : 1. الكشف الجزيئي ل HLA - B27 عند المرضى المصابين بالتهاب الفقار المقسط بواسطة طريقة تفاعل البلمرة المتسلسل بالوقت الحقيقي.2. المقارنة بين التقنيات المصلية والجزيئية في التحقق من حالة HLA - B27.3. تقييم علاقة HLA - B27 مع بعض المتغيرات السريريه.المرضى والمواد وطرق العمل : اجريت دراسة مستعرضة على 83 مريضا عراقيا بالغا شخصوا في عيادة استشارات الروماتيزم والتاهيل في مدينة الطب - بغداد / العراق. واجريت تقارير بيانات المرضى والاختبارات اللاحقة بعد موافقة المريض. سجلت البيانات الديموغرافية والسريرية لجميع المرضى. حددت frequency of HLA - B27 لجميع المرضى المسجلين بطريقة تفاعل البلمرة المتسلسل بالوقت الحقيقي باستخدام HLA - B27 RealFast™ kit وكذلك بطريقة مقايسة الممتز المناعي المرتبط بالانزيم (ELISA) باستخدام (human leukocyte antigen) ELISA kit.سحبت 3 - 5 مل عينات دم من المرضى الداخلين بالدراسة وقسمت الى عينتين : 1.5 - 2 مل جمعت في الانبوب العادي plane tube وتركت لتتجلط لاختبار (ELISA) والذي اجري في المختبرات التعليمية في مدينة الطب، وجمعت عينة دم اخرى بحجم 2.5 - 3 مل في انبوبK2 EDTA tube وحفظ مجمدا لتحليل الحمض النووي الذي اجري في المختبر المركزي للصحة العامه.النتائج : كان متوسط عمر المريض اعمار المرضى (±الانحراف المعياري) هو 38.4±9.8 سنة (المدى 16 - 62 سنه)، ونسبة الذكور الى الاناث هي 10.86/1، المرضى الذكور كان عددهم 76 ومرضى الاناث 7. متوسط عمر المرض من المرضى هو 9.08±32.13 سنة وكان متوسط عمر المرض للمرض هو 6.5±5.8 سنه. لوحظ التهاب الارتكاز في 44 (53%)، كان التهاب المفاصل الاطراف حاضرا في 14 (16.9%)، وكان التهاب العنبة موجودا في 12 (14.5%)، وامراض القلب والاوعية الدموية والصدفية وجدت في مريض واحد (1.2%) لكل منهما. كشف وجود HLA - B27 في 55 )66.3%) من المرضى من خلال real time PCR و28 (33.7%) خالة المتبقية كانوا حالات سلبية لـHLA - B27. كان ظهور المرض مبكرا وكانت مدة المرض اطول في المرضى الايجابيين لـHLA - B27 (قيمة p <0.05 لكل منهما). كانت هناك علاقة ذات دلالة احصائية بين HLA - B27 الايجابية مع الجنس والتهاب العنبة (p= 0.002, 0.032، على التوالي)، ولم تكن هناك ارتباطات ذات دلالة احصائية لـ HLA - B27 الايجابية مع العمر للمريض عند التشخيص ، وتاريخ العائلة، والتهاب الارتكاز ، والتهاب مفاصل الاطراف ، وESR وCRP والنتائج الاشعاعية وانواع العلاج. اظهرت حالة sHLA - B27 بواسطة ELISA لـ 83 حالة 32 حالة ايجابية (38.6%). عند مقارنة نتائج كلا الاختباريين باستخدام نتائج PCR كسيطرة، كانت 11 حالة نتائج ايجابية كاذبه و34 حالة كانت نتائج سلبية كاذبه، حساسية ELISA هي 38.18% والخصوصية 60.71%، وهذه الطريقة تكشف عن HLA - B27 القابل للذوبان، لذلك يمكن ان تتاثر بعدة عوامل بما في ذلك العلاج المستمر للمريض وصحة المريض. من بين 83 حالة، هناك 28 حالة (33.7%) لديها اختبار المصلي الاولي للكشف عن HLA - B27 باختبار microlymphocytotoxicity (MLCT)؛ اوضحت المقارنة بين نتائج ELISA وMLCT مع real time PCR (المستخدم كسيطرة) ان طريقة ELISA اظهرت 13 (46.4%) حالة هي نتائج سلبية كاذبة و3 (10.7%) حالات هي نتائج ايجابية كاذبة (حساسية = 60.6% ، خصوصية = 72.72% ، القيمة التنبؤية الايجابية (PPV)= 86.95%) في حين MLCT اظهرت 9 (32.1%) حالة كانت نتائج سلبية كاذبة و2 (7.1%) حالة هي نتائج ايجابية كاذبة (حساسية = 68.96% وخصوصية = 80% ، PPV = 90.9%). الاستنتاجات : • يتفوق كشف real time PCR لحالة HLA - B27 على التقنيات المصليةMLCT) و(ELISA. الاعتمادية والراحة والفعالية من حيث التكلفة لهذة الطريقة جعلها قابلة للتطبيق على التنميط الجيني HLA - B27 في الممارسات الروتينيه.• اعطت MLCT نتائج افضل من ELISA حيث استخدم الاخير للكشف عن sHLA - B27. كلا التقنيتين تحمل نتائج ايجابية كاذبة وسلبية كاذبة عاليه.• في المختبرات التي لاتتوفر فيها التشخيصات الجزيئيه، يمكن استخدام طريقة MLCT مع استخدام مضخمات صفيحة كبيرة (large panel antisera) لتقليل النتائج الكاذبه. • لم يؤدي الكشف عن ELISA لـsHLA - B27 في المصل الى الحساسيه او التحديد المطلوب. لتعزيز الايجابية، يوصى استخدام عينات البلازما ومحلول الاستعزاز(enhancing solution) لمنع فقدان sHLA - B27 اثناء التخثر وكذلك لمنع التفاعل المتصالب (cross - reaction) مع HLA - B7.• هناك ارتباط قوي بين HLA - B27 والتهاب الفقار المقسط بين المرضى العراقيين. • هناك ميل للمرض للتاثير على الرجال الايجابي HLA - B27 اكثر من النساء الايجابية HLA - B27.• تظهر اعراض AS في وقت اسبق في وجود جين HLA - B27 ومدة المرض كانت اطول في مرضى HLA - B27 الايجابيين من المرضى السلبيين، ولا يوجد اي ارتباطات مهمة لـ HLA - B27 مع المتغييرات المدروسة الاخرى.• هناك غلبة الذكور في الحالات AS وتبدا الاعراض عادة في الشباب | Ankylosing spondylitis (AS) is a chronic inflammatory disease affecting the sacroiliac joints, spine and not infrequently peripheral joints. AS affects 0.1 - 1% of general population. The exact cause of AS is still unknown but a strong genetic predisposition for AS was determined with a strong association with HLA - B27 positivity. HLA - B27 is present in >90% of white AS patients and 50 - 80% of non - white AS patients. There are several methods for the detection of HLA - B27 as serological methods (microlymphocytotoxicity (MLCT) and enzyme linked immunosorbent assay (ELISA)) and molecular techniques (DNA - based e.g. PCR). Serological methods were at first the standard tests for detection of HLA - B27 antigens but were replaced by DNA - based HLA typing because serologic methods have several disadvantages e.g. being sensitive to down - regulation or conformational changes of the antigens, cannot detect the protein structure differences caused by single or limited nucleotide polymorphism, have false - negative or false - positive results for several causes such as cross reactivity to other antibodies with different HLA class I antigens due to the extensive similarity within the class, altered antigenic epitopes, platelet or erythrocyte contamination. These changes to the protein (antigen) structure occur frequently in response to concurrent infection, drug therapy and health state of the patient.PCR - based techniques are not affected by these protein changes and are thus more representative to the state of the gene product (present / absent); in addition, it can detect allelic differences at the nucleotide level. It is a rapid and a selective method for the detection of HLA - B2701 to HLA - B2728 subtypes; it is capable of detecting a single base difference in DNA sequence between the two alleles and can be applied on any source of good quality DNA regardless of cell viability.Aims of the study : • To detect HLA - B27 status among patients with ankylosing spondylitis using real - time PCR technique.• To compare serologic and molecular techniques in verifying HLA - B27 status, and• To assess the correlation of HLA - B27 status with some clinical variables.Patients, Materials and Methods : A cross - sectional study was conducted on 83 adult Iraqi patients with AS diagnosed at the Rheumatology and Rehabilitation Consultation Clinic at the Medical City - Baghdad / Iraq. Patients’ data reporting and subsequent testing was performed following patients’ consents. Demographic and clinical data of all patients were reported. The frequency of HLA - B27 was determined in all enrolled patients by real - time polymerase chain reaction (PCR) using HLA - B27 RealFast™ kit as well as by ELISA method using (human leukocyte antigen) ELISA kit. Three to five ml of peripheral blood sample was aspirated from each and every enrolled patient. It was divided into two samples : 1.5 - 2 ml was collected in a plain tube and left to clot for serological (ELISA) test which was performed at The Teaching Laboratories / Medical City Campus and another 2.5 - 3 ml sample was collected in K2 EDTA tube and was kept frozen for DNA analysis which was performed at the Central Public Health Laboratory.Results : The mean age of patients (± SD) was (38.4±9.8) years (range 16 - 62 years). Male to female ratio was (9 : 1), male patients were 76 and female patients were 6. The mean age of disease onset of patients was 32.13±9.08 years and the mean age of disease duration of patients was 6.5±5.8 years. Enthesitis was observed in 44 (53%), peripheral arthritis was present in 14 (16.9%), eye involvement was present in 12 (14.5%), cardiovascular disease and psoriasis were in 1 (1.2%) each.HLA - B27 positivity was detected in 55 (66.3%) patients by real - time PCR and remaining 28 (33.7%) cases were negative HLA - B27. The disease onset was earlier and disease duration was longer in HLA - B27 - positive patients (p - value<0.05 for both). There were statistically significant correlation between HLA - B27 positivity with gender and uveitis, (p= 0.002, 0.032, respectively) and there were no statistically significant associations of HLA - B27 positivity with age at diagnosis, family history, enthesitis, peripheral arthritis, ESR, CRP, radiological findings and treatment types. The sHLA - B27 status by ELISA test for 83 cases showed 32 (38.6%) positive cases. Comparing the results of both tests using real - time PCR results as a control, 11 HLA - B27 cases were false positive and 34 patients were false negative results. Thus, the sensitivity of ELISA was 38.18% and specificity was 60.71%, this method detects soluble HLA - B27, so it can be affected by several factors including ongoing patient’s treatment and patient’s health. Out of 83 cases, 28 (33.7%) cases have initial serological test for detection of HLA - B27 by microlymphocytotoxicity (MLCT) test; the comparison of ELISA and MLCT results with real - time PCR (used as a control) revealed that ELISA method showed 13 (46.4%) false negative results and 3 (10.7%) false positive results, (sensitivity=60.6%, specificity=72.72%, positive predictive value (PPV)=86.95%) while MLCT showed 9 (32.1%) false negative results and 2 (7.1%) false positive, positive (sensitivity =68.96% and specificity =80%, PPV=90.9%).Conclusions : • Real - time PCR detection of HLA - B27 status is superior to serological techniques (ELISA and MLCT). The reliability, convenience and cost effectiveness of this method make it applicable to HLA - B27 genotyping in routine practices.• MLCT yielded better results than ELISA when the latter was used for detection of sHLA - B27. Both serologic techniques carry high false positive and negative results.• In labs where molecular diagnostics are not available, MLCT test can be used instead adding large panel antisera to reduce false results.• ELISA detection of sHLA - B27 in serum did not give the required sensitivity or specificity. To enhance positivity, the use of plasma samples and an enhancing solution are recommended to prevent the loss of sHLA - B27 during clotting and to prevent cross reaction with HLA - B7.• There is a strong association between HLA - B27 and ankylosing spondylitis among Iraqi patients.• There is a tendency of the disease to affect HLA - B27 positive men more than HLA - B27 positive women.• Symptoms of AS appear earlier in the presence of HLA - B27 gene and the duration of the disease was longer in HLA - B27 positive than negative patients. Uveitis is more likely to occur in HLA - B27 positive than negative patients, and there are no significant associations of HLA - B27 with the other studied variables. • There is a male predominance in AS cases and the symptoms start usually in young adults.

هرمون مضاد مولريان كعلامة للحمل بعد عمليات التلقيح المجهري عند النساء اللاتي يعانين من متلازمة تكييس المبايض == Anti - Mullerian Hormone as a Marker for Pregnancy following Intracytoplasmic Sperm Injection in Women with Polycystic Ovarian Syndrome

Author name: انعام عبد الواحد ذيب
Supervisor name: عفان عزت حسن | باسل عويد محمد صالح
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: العقم هو مرض من امراض الجهاز التناسلي يقصد به عدم قدرة الزوجين على الانجاب او تحقيق الحمل السريري بعد 12 شهرا او اكثر من الجماع الطبيعي الغير المحمي. هرمون مضاد مولريان (AMH) هو البروتين الذي يرتبط هيكليا الى اكثر من 35 الببتيدات مثل انهيبين، اكتيفين، وبروتينات العظام الشكلية وعوامل تمايز النمو، وكثير من هذه الببتيدات تشارك في الوظيفة الانجابية في كلا الجنسين. هرمون مضاد مولريان يمثل علامة بيولوجية للحجم النسبي لاحتياطي المبيض، وعدد الخلايا في الاحتياطي الجريبي الذي يساعد في التقنيات المساعدة على الانجاب ويمكن استخدامها للتنبؤ بتوقيت انقطاع الطمث. قد يكون هرمون مضاد مولريان مفيد كعلامة لضعف المبيض في متلازمة تكيس المبايض، حيث تعكس مستويات هرمون مضاد مولريان عدد الجريبات النامية من المبيض. وهومفيد ايضا في التنبؤ ببعض حالات عجز المبيض في متلازمة تكيس المبايض لكون مستويات هرمون مضاد مولريان تعكس عدد جريبات المبيض النامية. وهو مفيد ايضا في التنبؤ في حالة عدم استجابة المبيض خلال عمليات التخصيب في المختبر او اطفال الانابيب (IVF). مستويات هرمون مضاد مولريان مفيدة في اتخاذ قرار بشان بروتوكولات التحفيز في عمليات اطفال الانابيب (IVF) ويساعد في تحديد عدد الاجنة التي يتم نقلها في عمليات التقنيات المساعدة على الانجاب لزيادة معدلات نجاح الحمل وتقليل خطر متلازمة فرط المبيض. اجريت هذه الدراسة في مستشفى كمال السامرائي لعلاج العقم واطفال الانابيب ومركز بغداد التخصصي لعلاج العقم واطفال الانابيب / بغداد - العراق، تم تقسيم مجموعات الدراسة الى مجموعتين، اللواتي لديهن متلازمة تكيس المبايض والمجموعة الثانية تتالف من الذين ليس لديهم تكيس المبايض (انثى عادية على ما يبدو مع عامل العقم ذكري)، وقد سجلت في هذه الدراسة المرتقبة ، خمسة واربعين مريضة تتراوح اعمارهن بين 18 و42 عاما يخضعن لعملية الحقن المجهري. وقد صممت الدراسة لمعرفة دور مستويات مصل الدم ومستويات السائل الجريبي( (FFمن هرمون مضاد مولريان(AMH)، الهرمون المنبه للجريب ((FSH ،الهرمون المنشط للجسم الاصفر( (LH،هرمون الاسترادايول (E₂) والانسولين في التنبؤ بالنتيجة السريرية (الحمل) بعد عمليات الحقن المجهري في النساء اللواتي يعانين من متلازمة تكيس المبايض واللواتي لديهم اباضة اعتيادية (عامل ذكري). ولاعداد قيم قطعية محددة لهرمون مضاد مولريان في مصل الدم والسائل الجريبي للاستفادة منها في التنبؤ بنتائج الحمل، وكدلك تقييم العلاقة بين النتائج التي تم الحصول عليها للهرمونات المدروسة في مصل الدم والسائل الجريبي. اظهرت نتائج هذه الدراسة وجود زيادة معنوية في مستوى هرمون مضاد مولريان في مصل الدم لدى النساء اللواتي يعانين من تكيس المبايض من النساء اللواتي لا يعانون من تكيس المبايض (P = 0.001)، فيما يتعلق بالهرمونات المقاسة الاخرى، اظهرت النتائج عدم وجود فرق معنوي بين مستويات الهرمونات في مصل الدم بين النساء اللواتي يعانين من تكيس المبايض من النساء اللواتي لا يعانين من تكيس المبايض (P = 0.034)، كما لوحظ وجود زيادة معنوية في مستوى هرمون الاسترادايول في مصل الدم في اليوم الثاني للدورة الشهرية لدى النساء اللواتي يعانين من تكيس المبايض من النساء اللواتي لا يعانين من تكيس المبايض (P = 0.025) فيما يتعلق بالهرمونات المقاسة الاخرى، كشفت النتائج عن عدم وجود فرق معنوي بين مستويات السائل الجريبي لهذه الهرمونات في النساء اللواتي يعانين من تكيس المبايض من النساء اللواتي لا يعانين من تكيس المبايض. عند اجراء المقارنة بين مستوى هرمون مضاد مولريان في مصل الدم وسائل الجريبات ومستويات الهرمونات الاخرى عند النساء اللواتي يعانين من تكيس المبايض والنساء اللواتي لا يعانين من تكيس المبايض من خلال قياس قيم الاختلافات المقترنة، كانت اكبر القيم في مستوى هرمون الاسترادايول ₂E بعده هرمون مضاد مولريان ، ثم هرمون المنبه للجريب (FSH) ، ثم الهرمون المنشط للجسم الاصفر (LH)،ثم الانسولين تعاقبا. عند اجراء دراسة القيم القطعية في مصل الدم والسائل الجريبي لهرمون مضاد مولريان لغرض التنبؤ بنتائج الحمل باستخدام منحنى النسب المئوية 90 و95 عند النساء اللواتي يعانين من تكيس المبايض والنساء اللواتي لا يعانين من تكيس المبايض وجميع المرضى في الدراسة، وهذا قد يساعد في التنبؤ بالنتائج السريرية (الحمل). لوحظ ارتباط مستوى هرمون مضاد مولريان مباشرة مع نتائج الحمل السريري. باستخدام غير الحوامل (الذين لديهم تكيس المبايض والذين لا يعانون من تكيس المبايض) بشكل عام لادخال مستوى قيم قطعية للتنبؤ بنتيجة الحمل. اظهرت مؤشرات حساسية وخصوصية هرمون مضاد مولريان والهرمونات الاخرى في المصل والسائل الجريبي ان مؤشر حساسية وخصوصية هرمون مضاد مولريان في المصل هي (79٪) وفي السائل الجريبي هي (70.3٪) وهما يمثلان اعلى قيمة مقارنة بالهرمونات المقاسة الاخرى، وهي هرمون الاسترادايول ₂E ، هرمون المنبه للجريب (FSH) ، الهرمون المنشط للجسم الاصفر (LH)، الانسولين ومقاومة الانسولين. ومن خلال دراسة منحنى روك للمصل والسائل الجريبي لهرمون مضاد مولريان وغيرها من الهرمونات وهي الاسترادايول ₂E ، هرمون المنبه للجريب (FSH) ، الهرمون المنشط للجسم الاصفر (LH)، الانسولين ومقاومة الانسولين ، تبين ان اكبر مساحة لما تحت المنحنى ((AUC كانت تعود لهرمون مضاد مولريان في المصل وبعدها لهرمون مضاد مولريان في السائل الجريبي. من هذه الدراسة نستنتج الاهمية السريرية لقياس هرمون مضاد مولريان في المصل وفي السائل الجريبي في تقييم النتائج السريرية (الحمل) بعد عمليات الحقن المجهري في النساء اللواتي تعانين من متلازمة تكيس المبايض ، ايضا ان قياس مستويات هرمون مضاد مولريان في المصل وفي السائل الجريبي مهمة في التنبؤ بحالات متلازمة تكيس المبايض في النساء التي تقوم بعملية الحقن المجهري، حيث ان زيادة مستويات هرمون مضاد مولريان تعكس شدة المرض. ان القيم القطعية التي تم الحصول عليها من تركيزات هرمون مضاد مولريان في المصل وفي السائل الجريبي مهمة جدا للتنبؤ بنتائج تقنية الحقن المجهري (ICSI) واستكمالها او انهاءها في (النساء اللواتي يعانين من متلازمة تكيس المبايض والنساء اللواتي لا يعانين منها ). | Infertility is a disease of the reproductive system refers to an inability of a couple to achieve a clinical pregnancy after 2 years or more of regular unprotected sexual intercourse. Anti - Müllerian hormone (AMH) is a protein that is structurally related to more than 35 peptides like inhibin , activin, bone morphogenetic proteins (BMPs) and growth differentiation factors, many of these involved in reproductive function in both sexes. AMH serves as a biomarker for the relative size of the ovarian reserve; the number of cells in the follicular reserve that helps in assisted reproduction and can be used to predict the timing of menopause. AMH may be useful as a marker of ovarian impairment in polycystic ovary syndrome, since AMH levels reflect number of the developing ovarian follicles. It is also useful in prediction of poor ovarian response during in vitro fertilization (IVF). AMH levels are useful in deciding what stimulation protocols for IVF cycles and helps in decisions about the number of embryos to transfer in assisted reproduction techniques to increase the pregnancy success rates and decreasing the risk of ovarian hyperstimulation syndrome. The study was designed to investigate the role of serum and follicular fluid levels of AMH, Follicular stimulating hormone (FSH), Luteinizing hormone (LH), Estradiol (E2) and insulin in prediction of clinical outcome (pregnancy) in women with PCOS undergoing intracytoplasmic sperm injection(ICSI) and those with normal ovulation and to set up the cutoff values of serum and follicular fluid AMH concentration for prediction of pregnancy outcome, and to evaluate the relationship between the obtained results of the studied women’s in blood serum and in follicular fluid (FF). This study was conducted in Kamal Al - Samarrai hospital for infertility treatment and IVF and in Baghdad Specialist Center for treatment of infertility and IVF/Baghdad - Iraq, the study groups were divided into two groups, those with polycystic ovarian syndrome (PCOs) and those without PCOs (normal female with male factor infertility), forty five female patients aged between 18 - 42 years undergoing IVF/ICSI treatment were enrolled in this prospective study. The results of this study showed a significant increase in serum AMH level of PCOs women’s than that of non PCOs women’s (0.001), in regard to other measured hormones, the results revealed that there was no significant difference between serum levels of these hormones in PCOs and non PCOs women’s. There was significant increase in follicular fluid (FF) AMH level of PCOs women’s than that of non PCOs women’s (P= 0.034).There was also a significant increase in FFE₂ level in non PCOs women’s than in PCOs women’s (P=0.025), in regard to other measured hormones, the results revealed that there was no significant difference between FF levels of these hormones in PCOs and non PCOs women’s. In comparison between the serum and follicular fluid ( FF) AMH level and other parameters in PCOs women’s and in non PCOs women’s by measuring the paired differences values, the greatest values was in the E₂ level after that the AMH, then the FSH, LH, Insulin consequently. By studying the cutoff values of the serum and follicular fluid AMH concentration for prediction of pregnancy outcome by using the percentiles 90 and 95 in PCOs, non PCOs and all patients in the study, this may help in prediction of clinical outcome (pregnancy). AMH was directly correlated with clinical pregnancy outcome. Using non pregnant (PCOs and non PCOs) in general to introduce a cutoff level for prediction of pregnancy outcome. The sensitivity and specificity index of serum and follicular AMH and other hormones, revealed that the sensitivity and specificity index of serum AMH (79%) and of follicular AMH (70.3%) have the highest value in comparison with other routine measured hormones, E₂, FSH, LH, Insulin and Insulin resistance. The Receiver operating characteristic (ROC) curve for serum and follicular fluid AMH and other parameters, E₂, FSH, LH, Insulin and Insulin resistance, the greater area was founded belong to serum AMH then the FF AMH. From this study concluded the clinical importance of the measurements of serum and FF levels of AMH in assessment of successful ICSI techniques in PCOS infertile women, also the measurements of serum and FF levels of AMH are important in prediction of cases of PCOS in women’s undergoing ICSI techniques. The obtained cut - off values of serum and FF AMH concentrations are very important for prediction of the outcome of the ICSI technique and its continuation or termination in (PCOs and non PCOs).

دراسة مقارنة بين الرياضيين وكثيري الجلوس من ناحية وظائف الرئة وتشبع الاوكسجين وتركيز الشوارد في الدم == Comparative Study of Pulmonary Functions, Oxygen Saturation, and Electrolytes in Athletes and Sedentary Controls at Rest

Author name: زياد طارق عبد الرزاق
Supervisor name: زيد عبد المجيد المدفعي
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: علم وظائف الاعضاء الرياضية هو دراسة الاثار طويلة المدى وقصيرة المدى للتدريب والظروف على الرياضيين، لمعرفة كيف يتفاعل رياضيا لانواع معينة من التمارين الرياضية.العضلات، والشوارد وتشبع الاكسجين تتاثر في الغالب في ممارسة. وفقا لنوع من الرياضة، ومدة التدريب والعضلات تتاثر في الغالب من قبل هذا التمرين، تتاثر قراءات الرئوية ايضا، وتميل الى ان تكون اعلى لدى الرياضيين من تلك التي من الناس المستقرة وبين المجموعات الرياضية انفسهم.1. هذه الدراسة وسط لتحديد احتراما في استجابة لاختبار وظائف الرئة، الشوارد وتشبع الاكسجين بين الرياضيين والمستقرة.2. التعرف على الفروق في استجابة لاختبار اداء الرئتين، وتشبع الاكسجين والشوارد بين الرياضيين من مختلف الرياضة الاكثر شعبية في العراق.اجريت قياس التنفس، ومستوى تشبع الاكسجين والدم الشوارد بما في ذلك الكالسيوم والمغنيسيوم والصوديوم والبوتاسيوم على 66 الرياضيين المستوى الوطني مختلفة تتراوح اعمارهم بين [24.36 ± 4.65 سنة]. وكان لاعب من خمس مجموعات رياضة مختلفة وكانوا جميعا من الذكور. للخروج منها، كان هناك 20 المصارعين، 15 لاعبي كرة القدم، 8 سباحين و8 عدائين و15 الكاراتيه مختلطة المقاتلين جميعهم رجال الرياضية المتخصصة، في حين كانت المستقرة 40 طلاب الطب، والذكور. وتبين هذه الدراسة ان الرياضيين لديها اعلى اختبارات وظائف الرئة من الجلوس في حين اظهر تشبع الاكسجين والشوارد في الدم في هذه الدراسة لا يوجد فرق كبير بين الرياضيين في الراحة وتلمس المستقرة | Sports physiology is the study of the long - and short - term effects of training and conditions on athletes, to learn how an athlete reacts to specific types of exercise.Muscles, electrolytes and oxygen saturation are mostly affected in exercise; and according to the type of sport, duration of training and muscles mostly affected by this workout, pulmonary readings are also affected and tend to be higher in athletes than those of sedentary people and between athletic groups themselves.1. This study is amid to Identify the deference in response of pulmonary function test, electrolytes and oxygen saturation between athletes and sedentary.2. Identify the differences in response of pulmonary function test, oxygen saturation and electrolytes between athletes of different most popular sport in Iraq.Spirometry, oxygen saturation level and serum electrolytes including calcium, magnesium, sodium and potassium were conducted on 66 different national level athletes aged [24.36 ± 4.65 years]. The athletes were from five different sport groups and were all males. Out of them, there were 20 wrestlers, 15 footballers, 8 swimmers, 8 runners and 15 karate mixed fighters all of them are specialized sports men, while sedentary were 40 medical students, males. This study shows that athletes have higher pulmonary function tests than sedentary while oxygen saturation and serum electrolytes in the present study showed

خلل الوظائف اللاارادية في مرضى الشلل الرعاشي == DYSAUTONOMIA IN PARKINSON'S DISEASE

Author name: اسماء خلف حمود
Supervisor name: اسراء فائق جعفر | فرقد بدر حمدان
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: يؤثر على كبار السن بشكل اساسي، ولكن تتاثر الفئة العمرية الشابة ايضا. انه ينتج عن موت او تحلل الخلايا العصبية المنتجة للدوبامين بشكل رئيسي في المادة السوداء وقد يمتد الى اجزاء اخرى من الدماغ تحتوي على هذه الخلايا العصبية . ان السبب الرئيسي لهذا المرض لا يزال غير معروف، على الرغم من ان هناك العديد من العوامل التي تساهم في حدوثه.احد هذه العوامل هو الالتهاب، الاكسدة، واختلال عمل بيوت الطاقة (الميتوكوندريا) بالاضافة الى الاستعداد الوراثي للمريض المصاب بهذا المرض.ان الاعراض المرتبطة بمرض باركنسون هي الاعراض الحركية ، غير الحركية وغيرها من الاعراض بما في ذلك اعراض اختلال الجهاز العصبي اللاارادي.تربط هذه الدراسة بين اعتلال الجهاز العصبي اللاارادي من مرض باركنسون مع مواد استيل كولين، ومضادات الاكسدة، وعامل نخر الورم في ثلاث مجموعات دراسية التي هي مرضى باركنسون مع خلل الوظائف التلقائية (اللاارادية)، ومرضى باركنسون دون خلل الوظائف التلقائية ومجموعات الاشخاص الاصحاء.يتم ذلك من خلال دراسة الفحوصات اللاارادية بما في ذلك فحوصات القلب والاوعية الدموية اللاارادية (نسبة فالسالفا، ونسبة 30 : 15، والتغير في ضغط الدم عند الوقوف)، واستجابة الجلد السمبثاوي (SSR)، بالاضافة الى اختبار الكيمياء الحيوية من مستويات الاستيل كولين، ومضادات الاكسدة، وعامل نخر الورم.فيما يتعلق الاختبارات اللاارادية، اظهرت النتائج ان التغيرات في ضغط الدم عند الوقوف كان لها دلالة احصائية بالاضافة الى ذلك، نقص ضغط الدم الوضعي يمثل اهم اعراض خلل الوظائف التلقائية في مرض باركنسون.اما بالنسبة لمستوى الاستيل كولين فقد انخفض مع تطور مرض الشلل الرعاشي وذلك باصابة جهاز الوظائف التلقائية (اللاارادية). اضافة لذلك فان مستوى مضادات الاكسدة لم يظهر اي دلالة احصائية.من ناحية اخرى، نسبة عامل نخر الورم يزداد بتتفاقم المرض ليشمل خلل الوظائف التلقائية اللاارادية.بينت الدراسة ان عامل نخر الورم وعامل شدة الاكسدة يؤثران على تقدم مرض باركنسون ككل وخلل الوظائف الاارادية لهذا المرض بشكل خاص. | Parkinson disease is regarded as a common neurodegenerative disorder in the whole world that affects mainly elderly people, but young age group are also affected. It results from dopaminergic neuronal degeneration mainly in substantia nigra that may extend to other parts of the brain. The main cause of this disorder still unknown, although many factors that contributes in its pathogenesis. Of these factors, there are inflammation, oxidative stress, and mitochondrial dysfunction in addition to genetic predisposition.Symptoms related to Parkinson’s disease are motor, non - motor. The present study relates the autonomic dysfunction of Parkinson disease with the acetylcholine, antioxidants, and tumor necrosis factor in three study groups which are Parkinson patients with autonomic dysfunction, Parkinson patients without autonomic dysfunction and the control groups. The autonomic assessment is done by studying the autonomic tests including cardiovascular autonomic study (Valsalva ratio, 30 : 15 ratio, blood pressure change with posture), and sympathetic skin response, in addition to biochemical testing of acetylcholine, antioxidant, and Tumor necrosis factor levels. Regarding autonomic testing, the results difference that is statistically significant regarding changes of blood pressure in response to posture, in addition Parkinson disease. Acetylcholine level decrease as the disease progress from Parkinson patients without autonomic dysfunction to Parkinson patients with autonomic dysfunction. While antioxidant level was not significant difference between Parkinson patients with and without dysautonomia. On the other hand, tumor necrosis factor increases as the disease worsen to involve autonomic dysfunction. This study shows the effect of tumor necrosis factor and oxidative stress affecting the pathogenesis of Parkinson disease as a whole and autonomic dysfunction in specific

استهداف الخلية السرطانية بواسطة نانو الهجين الحويصلي كنهج جديد لموت الخلايا المبرمج == Targeting Cancer Cell by Nano Hybrid Liposome : A new Approach for apoptosis

Author name: نورا عبد عواد
Supervisor name: قسمة محمد تركي | ناهي يوسف ياسين
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: الخلفية : يعتبر العلاج الهادف للسرطان بالنانو حقلا مهما للبحث عن علاجات خاصة بالسرطان لتجنب الاعراض الجانبيه للادويه الكيمياويه التقليديه ،ولان خلايا السرطان تعاني من عطل في منظومة الموت المبرمج يؤدي هذا الى تفضيلهه لمسارات البقاء على قيد الحياة ومقاومة الادويه ولهذا يعتبر تحفيز الموت المبرمج في الخلايا السرطانية من الوسائل المفضلة للتطبيق في اي دواء مستحدث لعلاج السرطان .ان اهداف العلاج بالنانو هو رفع الكفاءة العلاجية للدواء وانتقائيتة والتغلب على مقاومة الخلايا له والتي تمثل عقبة في علاج السرطان.وقد يفيد استعمال الاجسام النانويه الدهنية المهجنة بكل هذه الاغراض في علاج السرطان .تتكون الاجسام النانوية الدهنية المهجنه من مع الايثر)C12(EO)23)L - DMPC والذي يتداخل انتقائيا مع جدار الخلية السرطانية مؤديا الى موتهاالاهداف : تقييم قابلية اجسام النانو الدهنيه المهجنه والمصنعه محليا على احباط نمو خلايا سرطان عنق الرحم والرابدومايوساركوما كتاكيد لحصول الموت المبرمج في زراعتها المحضونه مع الهجين الحويصلي المصنع مختبريا.طرق العمل حضرت اجسام النانو الدهنية الهجينة من مزج [DMPC & C12(E0)23] ومزجها في وسط زررعي خاص ثم بعد ذلك يتم وضعها في حمام مائي لامواج فوق الصوتية Sonicatore) ) للحصول على الشكل الكروي والحجم النانوي الهجين وللتاكد من ذلك تم فحصها باستخدام المجهر الالكتروني الماسح (SEM) ومجهر القوة الذرية (AFM) , وتم تحديد حيوية الخلايا عن طريق احتساب نسه الخلايا الميتة الى الحية بعد تصبيغهه بالصبغات التي تبين سمية الخلايا Crystal Violate&MTTوتم فحص والتاكد من الموت المبرمج بتقنيات متعدده منها الصبغات المتفلوره واختلال شدة نفاذية المايتوكوندريا وقياس فعاليه انزيم PCR وال, (Caspase - 3) النتائج : النتائج تمكن الهجين الحويصلي الواقع ي مجال وانتقائية النانو من احباط انقسام خلايا سرطاني عنق الرحم، والرابدومايوساركوما وبتركيز نصفي (IC50) مساوي لــ (0.2 mmol)، بينما لم تتاثر الخلايا اللمفاوية الطبيعية بهذه الظروف.وكان التاثير واضحا في كل الطرق التي استعملت لقياس الموت المبرمج وقدمت ادله واضحة على ان الهجين الحويصلي قادر على تحفيز الموت المبرمج في خلايا سرطاني عنق الرحم والرابدمايوساركوما عند حضنها مع الجرعه المحبطه النصفية للهجين الحويصلي ولمدة 24 ساعه .الاستنتاجات : يمكن اعتبار الهجين الحويصلي المصنع كوسيلة ممكنه للعلاج بالنانو وباقل اضرار جانبيه للخلايا الطبيعيه مقارنة مع العلاجات المستعمله الاخرى. وتوصي هذه الدراسة ببحث طريقة عمل الهجين الحويصلي وقابليته على احباط نمو انواع اخرى من الخلايا السرطانيه في المختبر وفي البحوث الحيه | Background : Cancer targeted nanotherapy represent an exciting field in the search for new cancer specific therapies to avoid conventional chemotherapy side effects. Because cancer cells usually have malfunctioning apoptotic machinery which favors survival pathways and drug resistance, cancer cell apoptosis is the favorable event to be induced in any new anticancer agent development. Nanotherapy goals are to elevate therapeutic efficiency, selectivity, and overcome drug resistance as major obstacle in cancer treatment. Hybrid nanoliposomes (nHLs) may fulfill all these features in cancer therapeutics.Hybridnanoliposomes (HLs) composed of L - α - dimyristoylphosphatidylcholine (DMPC) and Polyoxyethylene (23) dodecyl ether (C12 (EO)23) can integrate selectively into the cancer cell membrane inducing cancer cell death.Objectives : to assesses the capacity of locally synthesized hybrid nanoliposome to inhibit the growth of cervix cancer cells (HeLa) andrabdomyosarcoma(RD)to confirm the event of apoptosis in HeLa and RD cell lines incubated with in - house synthesized and characterized nHLs.Methods : hybrid nanoliposomes(nHLs) synthesized by sonication method from a mixture of DMPC and C12(EO)23 in tissue culture media RPMI - 1640 for 6 hours at 300W and 40ºC then filtration with 0.2µm filter. Shape and size characterized with scanning electron microscope (SEM) and atomic force microscope(AFM). Viability of HeLacell,RD cell, and normal lymphocytes challenged with HLs were determined using MTT and Crystal violate assay. Induction of apoptosis in the challenged cells was examined by staining with fluorescence dye mix cridine orange/propidium iodide,cell gel electrophoresis, mitochondrial membrane potential disruption, caspase - 3 activity and real time PCR.Results : synthesized nHLs were in nanozise range and selectively inhibited HeLa cells proliferation with IC50 of 0.2mM with no effect against normal lymphocytes. Apoptosis was evident ofHeLa cells population and RD was evident of treated with nHLs.All apoptosis detection procedures used gave a clear defined significant indication that nHLs was capable of inducing apoptosis in HeLa and RD cells incubated with the IC50.Conclusion : synthesized nHLs may considered as promising nanotherapy, this study recommends further inspections for the mechanism of action of nHLs and there capabilities to inhibit other types of cancers both invitro and in vivo.

التعبير المناعي النسيجي الكيميائي لبروتينات (CD24, CD44 وALDH1) للخلايا الجذعية السرطانية في سرطان الثدي : دراسة سريرية مرضية == Immunohistochemical Expression of Stem Cell Markers CD24, CD44 and ALDH1 in Invasive Breast Carcinoma A Clinicopathological Study

Author name: حسنين غسان حسين
Supervisor name: سالم رشيد حمودي العبيدي
General topic: Medicine
Specific topic: Tissue Diseases
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: يعد سرطان الثدي واحدا من الاورام السرطانية الاكثر شيوعا في النساء بالعالم يوقدر بحوالي 1,7 مليون حالة جديدة مسجلة في عام 2012 (بنسبة 11,9% من باقي الاورام السرطانية). في العراق سرطان الثدي يعتبر الاكثر شيوعا لدى النساء ويشكل ما نسبته 34,8% من بين باقي الاورام السرطانية (سجلات وزارة الصحة). الخلايا الجذعية السرطانية تعرف بانها : مجموعة صغيرة من الخلايا السرطانية داخل الورم السرطاني تعتبر المخزون لاعادة تكاثر الخلايا السرطانية وتكوين تنوع خلوي ذات تخصص مختلف في مراحل نموها, من المؤشرات المقبولة للخلايا الجذعية السرطانية هي : CD24 , CD44 , CD133 , EPCAM وALDH1).الهدف من الدراسة : تقييم التعبير المناعي النسيجي الكيميائي لمؤشرات الخلايا الجذعية السرطانية (CD24 , CD44 وALDH1) في كل من النسيج الطبيعي للثدي , الورم الغدي الليفي الحميد في الثدي وسرطان الثدي (بنوعيه سرطان الثدي القناتي الاختراقي وسرطان الثدي الفصي الاختراقي) وعلاقة هذا التعبير المناعي النسيجي الكيميائي مع المتغيرات السريرية المرضية (العمر , حجم الورم السرطاني , درجة التمايز للورم السرطاني وحالة الغدد اللمفاوية).المرضى , المواد وطرق العمل : من تاريخ نيسان 2012 الى اذار 2013 , تم اخذ 133 عينة نسيجية تشمل 37 عينة نسيجية للمجموعة الغير مصابة بالورم السرطاني ( 12 عينة لنسيج طبيعي للثدي و25 عينة للورم الحميد ) و96 عينة نسيجية للمجموعة المصابة بالورم السرطاني ( تتالف من 51 حالة رفع ثدي كامل و45 عملية استئصال للورم) حيث تكونت من 87 حالة من سرطان الثدي القناتي الاختراقي وتسع حالات من سرطان الثدي الفصي الاختراقي. اخذت الانسجة الارشيفية المطمورة في شمع البارافين للمدة بين 2011 و2013 من ملفات المختبرات التعليمية لدائرة مدينة الطب ومختبر الغدق التخصصي الخاص ومن معهد الطب العدلي في بغداد. وكل المعلومات السريرية تم استحصالها من البيانات الخاصة بالمرضى. كل العينات اعيد تقيمها بصبغة الهيماتوكسلين والايوسين وتم التحري عنها بواسطة الصبغة المناعية النسيجية الكيميائية للمؤشرات (CD24 , CD44 وALDH1).النتائج : وجد انه هناك علاقة ذات دلالة احصائية بين الحالة الصحية للثدي (ان كان الثدي طبيعي , به ورم حميد او ذات ورم سرطاني) والتعبير المناعي النسيجي الكيميائي للمؤشرات الثلاثة للخلية الجذعية السرطانية (CD24, CD44 وALDH1).التعبير المناعي النسيجي الكيميائي لمؤشر CD24 في نسيج الثدي : عشرة حالات من اصل 37(27%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. بالنسبة للمجموعة المصابة بالورم السرطاني , 41 حالة من اصل 87(47,1%) من حالات سرطان الثدي القناتي الاختراقي واربع حالات من اصل 9(44,4%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي موجب. ولم تكون العلاقة ذات دلالة احصائية بين مؤشر (CD24) والمتغيرات السريرية المرضية (العمر, حجم الورم, درجة التمايز وحالة الغدد اللمفاوية).التعبير المناعي النسيجي الكيميائي لمؤشر CD44 في نسيج الثدي : ثمانية حالات من اصل 37(21,6%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. اما ما يخص المجموعة المصابة بالورم السرطاني, 27 حالة من اصل 87(31,03%) من حالات سرطان الثدي القناتي الاختراقي وثلاث حالات من اصل 9(33,3%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي النسيجي موجب. توجد علاقة ذات دلالة احصائية بين مؤشر CD44 للخلايا الجذعية السرطانية مع حالة الغدد اللمفاوية حيث كانت قيمة (P) تساوي (0,0140). ولم يكن هنالك علاقة ذات دلالة احصائية بين مؤشر (CD44) وباقي المتغيرات السريرية المرضية (العمر, حجم الورم ودرجة التمايز).التعبير المناعي النسيجي الكيميائي لمؤشر ALDH1 في نسيج الثدي ينقسم الى : 1. التعبير المناعي لمؤشر ALDH1 في نسيج الخلايا السرطانية : سبعة حالات من اصل 37(18,9%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. وفيما يخص المجموعة المصابة بالورم السرطاني, 34 حالة من اصل 87(39,2%) من حالات سرطان الثدي القناتي الاختراقي وثلاث حالات من اصل 9(33,3%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي موجب. توجد علاقة ذات دلالة احصائية بين مؤشر ALDH1 للخلايا الجذعية السرطانية مع حالة درجة تمايز الورم حيث كانت قيمة (P) تساوي (0,0323). ولم يكن هنالك علاقة ذات دلالة احصائية بين مؤشر ALDH1 وباقي المتغيرات السريرية المرضية (العمر, حجم الورم وحالة الغدد اللمفاوية).2. التعبير المناعي لمؤشر ALDH1 في النسيج المحيط للخلايا السرطانية : ثمانية حالات من اصل 37(21,6%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي متوسط الى قوي التعبير في الخلايا المحيطة للخلايا السرطانية و29(78,4%) حالة يكون التعبير المناعي منعدم الى ضعيف في هذه الخلايا. اما ما يخص مجموعة المصابة بالورم السرطاني للثدي, 47 حالة من اصل 87(54,2%)من حالات سرطان الثدي القناتي الاختراقي وخمس حالات من اصل 9(55,6%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير متوسط الى قوي. من هذه الدراسة وجد انه لايوجد علاقة ذات دلالة احصائية بين التعبير المناعي لمؤشر ALDH1 في النسيج المحيط للخلايا السرطانية والمتغيرات السريرية المرضية (العمر, حجم الورم, درجة التمايز وحالة الغدد اللمفاوية).الاستنتاج : - توجد علاقة ذات دلالة احصائية بين مجموعة الغير مصابة بالورم السرطاني والمجموعة المصابة بالورم السرطاني للثدي من حيث التعبير المناعي للمؤشرات (CD24, CD44 وALDH1) في الخلايا الجذعية السرطانية. - هنالك علاقة ذات دلالة احصائية بين فرط التعبير المناعي لمؤشر CD44 وحالة الغدد اللمفاوية في حالات سرطان الثدي. - في هذه الدراسة توجد علاقة ذات دلالة احصائية بين فرط التعبير المناعي لمؤشر ALDH1 في الخلايا السرطانية ودرجة التمايز في الورم السرطاني للثدي. - نتائج المتحصلة من هذه الدراسة تقترح بان فرط التعبير المناعي النسيجي الكيميائي للمؤشرات المناعية (CD24, CD44 وALDH1) قد ترتبط بحالة تحول الورم الى النوع السرطاني وكذلك بالحالة البايولوجية لسرطان الثدي. | Breast Cancer is the most frequent diagnosed cancer among women worldwide with an estimated 1.7 million new cancer cases diagnosed in 2012 (11.9% of all cancers). In Iraq, cancer of the breast is the commonest cancer in females, in 2010 constituted 34.8% of all other malignancies in women. Cancer stem cells have been defined as - a small subset of cancer cells within a cancer that constitute a reservoir of self - sustaining cells with the exclusive ability to self - renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor, the well - accepted cancer stem cell markers are CD44, CD24, CD133, CD166, EpCAM and ALDH1.Aim of the studyTo assess the immunohistochemical expression of CD24, CD44 and ALDH1 as stem cell markers in normal breast tissue, fibroadenoma and breast carcinoma (Invasive ductal and Invasive lobular carcinomas), in correlation with some clinicopathological parameters (Age of patient, tumor size, tumor grade and lymph node status).Patients, materials and methods From April 2012 to March 2013, total of (133) tissue samples of surgical breast biopsies were included in this retrospective study, 37 cases of non - malignant breast cases (12 cases of normal breast tissue and 25 fibroadenoma case) and 96 cases of malignant breast cases (51 mastectomy case and 45 cases of excisional biopsies) were all of them invasive breast carcinoma, eighty seven cases were of invasive ductal carcinomas and nine cases of invasive lobular carcinomas. The samples were obtained from archival paraffin embedded blocks for the years (2011) to (2013) from the histopathology files of department of pathology in Teaching Laboratories of medical city Hospital, Al - Ghadaq’s private Laboratory and Institute Of Forensic Medicine in Baghdad.. The histopathological diagnosis had been revised and all cases were stained by immunohistochemical technique with CD24, CD44 and ALDH1 tumor markers.Results There was significant difference among the three states of health status (normal breast cases, fibroadenoma cases and invasive breast carcinoma) and immunohistochemical expression of stem cell marker (CD24, CD44 and ALDH1 epithelial and stromal expression). Regarding Immunohistochemical expression of CD24, ten cases out of 37(27%) of non malignant breast cases were positive of CD24 expression. For malignant breast cases, forty one invasive ductal carcinoma cases out of 87(47.1%) and four invasive lobular carcinoma cases out of 9(44.4%) were CD24 with positive expression, and there was no significant difference regarding CD24 expression in relation to different clinicopathological variables (age of the patient, tumor grade, lymph node status and tumor size). For CD44 immunohistochemical expression, eight cases out of 37(21.6%) of non malignant breast cases were positive of CD44 expression. For malignant breast cases, Twenty seven invasive ductal carcinoma cases out of 87(31.03%) and three invasive lobular carcinoma cases out of 9(33.3%) were positive CD44 expression, and there was a significant difference regarding CD44 expression in relation to lymph node status of the patients (P=0.0140). Regarding the age of the patient in invasive breast carcinomas there was no significant difference between CD44 expression and the three groups of the patient age, so as for grade of tumor and tumor size (P > 0.05). Regarding ALDH1 epithelial immunohistochemical expression, seven cases out of 37(18.9%) of non malignant breast cases were positive of CD44 expression, while for malignant breast cases, thirty four invasive ductal carcinoma cases out of 87(39.2%) and three invasive lobular carcinoma case out of 9(33.3%) showed a positive expression of epithelial ALDH1. There was no significant difference in epithelial ALDH1 expression between different age groups of the patients. CD44 immunohistochemical expression was significantly different in different grades of invasive breast carcinomas (P=0.0323). The mean value of ALDH1 - E expression showed no significant difference between cases with positive lymph node involvement and those with a negative lymph node involvement, also there was no significant correlation in epithelial ALDH1 immunohistochemical expression in relation to the tumor size. For stromal ALDH1 immunohistochemical expression, eight cases out of 37(21.6%) of non malignant breast cases were moderate/strong of stromal ALDH1 expression and 29(78.4%) cases were none/weak expression. Regarding malignant breast cases, forty seven invasive ductal carcinoma cases out of 87(54.2%) and five invasive lobular carcinoma cases out of 9(55.6%) were moderate/strong expression of stromal ALDH1, and there was no significant difference regarding stromal expression of ALDH1 in relation to different clinicopathological variables (age of the patient, tumor grade, lymph node status and tumor size). Conclusions • A statistical significant correlation has been found between Malignant Breast cases and Non Malignant Breast cases regarding the Immunohistochemical over expression of stem cell markers (CD24, CD44 and ALDH1 epithelial and stromal Expression).• There was a statistical significant correlation between CD44 immunohistochemical expression and lymph node status in invasive breast carcinomas cases.• A statistical significant correlation has been found between ALDH1 Epithelial immunohistochemical expression and Grade of tumor in cases of invasive breast carcinoma. • The results of this study suggest that higher stem cell markers (CD24, CD44 and ALDH1) expression may be associated with malignant transformation and progression in breast cancer biology

تقييم كهروتشخيصي لمرضى اعتلال جذور الاعصاب العنقية == ELECTRODIAGNOSTIC ASSESSMENT IN PATIENTS WITH CERVICAL RADICULOPATHY

Author name: زينه سليم فخري
Supervisor name: نجيب محمد | احسان محمد
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Cervical radiculopathy can be defined as pain in a radicular pattern in one or both upper extremities related to compression and/or irritation of one or more cervical nerve roots. In the electrodiagnostic laboratory, various types of electrodiagnostic studies may be considered when evaluating a patient for cervical radiculopathy. Potential tests including (needle electromyography, motor and sensory nerve conduction studies, late responses, and somatosensory evoked potentials) serves to confirm the presence of radiculopathy, establish the involved nerve root level, determine if axon loss or conduction block is present, grade the severity of the process, estimate the age of the radiculopathy and exclude other peripheral nerve diseases that mimic radiculopathy.The aim of this study was to approve the usefulness of the electrodiagnostic studies (NCS, EMG and SSEP) in the diagnosis of cervical radiculopathy, asses the role and accuracy of the SSEP test (specifically spinal N13 component) in the diagnosis of cervical radiculopathy, identify the extent and distribution of cervical paraspinal muscles denervation in cervical radiculopathy and match the electrodiagnostic results with imaging data in the localization of the root lesion involved.This is case control study had been conducted during the period from April 2016 to October 2017. Ninety five (95) patients with cervical radiculopathy with a mean age of (48.4±11) years and twenty six (26) healthy control subjects with a mean age of (45.2±10.1) years involved in the study. Each subject submitted for medical history, clinical examination and electrophysiological study using Nicolete Viking Quest , EMG, NCS, EP measuring system.XIIFor SNCS, Onset latency, SNAP amplitude and conduction velocity, while for MNCS, distal motor latency, CMAP amplitude and conduction velocity, in addition to the minimal F wave latency (that assess the late response) of median and ulnar nerves are performed.Whereas for right and left sides median nerve SSEP test, the following peak and interpeak latencies (1) N9; (2) N13; (3) N20; (4) N9 - N20; (5) N9 - N13 ; (6) N13 - N20 are measured.Concerning the needle EMG, the following parameters; insertional activity, spontaneous activity and motor units action potential characteristics (duration and phases) of the right and left trapezius, rhomboids major, deltoid, biceps, triceps, extensor indicis and first dorsal interosseous muscles are considered and recorded. In addition to the assessment of the spontaneous activity of the cervical paraspinal muscles.The results of this study revealed that there are significant differences; higher values for the patients when compared with that of control groups regarding Rt and Lt sides N13 peak latency, N9 - N13 and N13 - N20 inter - peak latency. Moreover, the sensitivity of SSEP components in comparison with the MRI (as a gold standard) is the higher for that of spinal N13.From the EMG and MRI findings, C6 and C7 cervical roots are the most roots affected in respect to C5, C8 or C4 roots.Consequently, there are mismatching in the percentages of cervical radiculopathies when diagnosed by the MRI of the cervical spine, needle EMG of the upper limbs muscles and that of the cervical paraspinal muscles, in such a way that Rt C7 radiculopathy 71.6% by cervical spine MRI mismatched with that of upper limbs EMG (66.3%) and 5.3% by the cervical paraspinal muscles.However, the percentages of each level of cervical radiculopathy proved to be higher by the combination of the EMG abnormaities of the cervical paraspinalXIIImuscles with that of the upper limbs muscles than by using the EMG of the paraspinal muscles or upper limbs muscles in an isolation (for Rt C6 radiculopathy 4.2% by paraspinal muscles EMG, 66.3% by upper limb and 68.4% by combination.Furthermore, the sensitivity of the EMG in cervical radiculopathy (using the MRI as a gold standard) increases by the combination of the abnormalities of the cervical paraspinal muscles with that of the upper limbs muscles, for example the sensitivity of Lt C6 radiculopathies 89.9% by upper limbs EMG, 93.2% by the combination of the EMG of PSM with that of the upper limbs muscles.In conclusion, traditional electrodiagnostic tests (NCS, EMG and SSEP) proved to be useful and complementary to the clinical examination in the evaluation of patients with cervical radiculopathy. The benefit of SSEP test for the evaluation of radiculopathies is based on the fact that signs and symptoms in radiculopathies can usually be related to injury of the afferent fibers and the SSEP test can monitor injury to these fibers.In addition to, spinal N13 is a sensitive technique suitable for detecting early and mild symptomatic cervical nerve root lesion.Moreover, cervical paraspinal muscles EMG is complementary to the upper limbs muscles EMG since that significant percentages of cervical radiculopathy will be missed if the examination of PSM is not part of the electrodiagnostic evaluation of patients with suspected radiculopathy. Furthermore, the sensitivity of the needle EMG is increased by the combination of the EMG of the paraspinal muscles with that of the upper limbs muscles, without paraspinal muscles needle EMG the screen of cervical radiculopathy misses much of its sensitivity. Finally, EMG and MRI are valuable and complementary diagnostic tools in the localization and extent of the cervical roots lesion.

التقييم الكهروفسلجي لالياف الاعصاب الصغيرة والكبيرة قبل وبعد الديلزة للمرضى المصابين بالعجز الكلوي المزمن == Electrophysiological Assessment of Small and Large Nerve Fibers Dysfunction Pre and Post Dialysis in Patients with Chronic Kidney Disease

Author name: فارس كاظم خضير
Supervisor name: نجيب حسن محمد
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: يتم التشخيص لمرضى الكلى المزمنة وظيفيا وتتتميز بالانخفاض التتدريجي لراجعة فيه في معدل الترشيح الكبيبي . ومما يزيد من تعقيدها هو عدم القدرة على الحفاظ على مستويات طبيعية من منتجات الايض للبروتين (اليوريا والكرياتينين) وكذلك الحفاظ على مستوى ضغط الدم الطبيعي واختلال في انتاج كريات الدم الحمراء والهيموغلوبين واختلال في معدلات الصوديوم والماء والاملاح . يعتبر اعتلال الاعصاب المحيطية من المضاعفات الشائعة للمرضى المصابين بالفشل الكلوي وهم معرضين اكثر من غيرهم للاصابة بالتهاب الاعصاب المحيطية المتعدد . الفحص الكهروفسلجي مهم اضافة الى الفحص السريري لاجل التشخيص المبكر لمثل هذه المضاعفات وكذلك للتمييز والتفريق بين الانواع المختلفة للاعتلال العصبي المحيطي. هدفت هذه الدراسة الى : (1) تقييم وظيفة الدراسة الكهروفسلجية في تشخيص الاعتلال العصبي للمرضى الذين يعانون من الفشل الكلوي المزمن .(2) معرفة القيم القياسية الاكثر ضرورية من دراسة توصيل العصب، ليتم تنفيذها كاختبار اولي في تشخيص الاعتلال العصبي الالياف الصغيرة.(3) الارتباط بين شدة الاعتلال العصبي المحيطي ومستوى الكرياتينين في الدم.ثلاث مجموعات من المواضيع المدرجة في هذه الدراسة : المرضى البالغين من مرض الكلى المزمن ليس على غسيل الكلى بعد، المرضى على غسيل الكلى ومراقبة الموضوعات.اجريت هذه الدراسة في وحدة الفسلجة العصبية في مستشفى اليرموك التعليمي، في الفترة من يونيو / حزيران 2016 الى سبتمبر / ايلول 2017.اجريت هذه الدراسة على ثلاثة مجاميع بكلا الجنسين تالفت المجموعة الضابطة من اثنان واربعين (42) متطوعا اصحاء (22 ذكور و20 اناث). تراوح عمر هذه الفئة بين 20 و75 سنة بمتوسط (52.48 ± 10.63) سنة. 22 - 75 سنة للذكور بمتوسط العمر (53.54 ± 14.57) سنة، و20 - 74 سنة للاناث بمتوسط العمر (51.42 ± 11.91) سنة. كان جميع الاشخاص الذين تم فحصهم من هذه المجموعة لا يعانوا من امراض مزمنة، مع عدم وجود تاريخ طبي من امراض الجهاز العصبي. لا يوجد تاريخ من تعاطي الكحول او تناول المخدرات. وقد كان اغلبهم من الاقارب العاديين، والعاملين الطبيين، والطلاب والعاملين . شملت الدراسة ثمانون مريضا يعانون من الفشل الكلوي المزمن. قسموا الى مجموعتين : ا - اربعون (40) مريضا مصابين بامراض الكلى المزمنة يراجعون عيادة امراض الكلية والغسيل الكلوي وهم غير خاضعين للعلاج بالديلزة ومعدل الكرياتنين في مصل الدم لديهم مرتفع بشكل مطرد اكبر من 2.5 ملغ / دلزمن كلا الجنسين (22 ذكور و18 اناث)، تراوحت اعمارهم بين( 35 و72 سنة) بمتوسط حسابي (55.35 ± 9.05) سنة.وتتراوحت مدة الاصابة بالاعتلال بين (2 - 10) سنوات، مع متوسط (8.72±3.65) سنواتب - اربعون (40) مريضا مصابين بامراض الكلى المزمنة خاضعين للعلاج بالديلزة الدموية من كلا الجنسين (21 ذكور و19 اناث)، تراوحj اعمارهم بين (43 - 70 سنة) بمتوسط (56.98 ± 7.40) سنة. تراوحت مدة الاعتلال بين (7 - 15) سنة بمتوسط (13.87 ± 5.41) سنة.تم تعريف جميع الاشخاص ال 122 (المرضى والسيطرة) في هذه الدراسة حول هدف وطبيعة الدراسة وتم استحصال موافقتهم على شمولهم بالدراسة. شملت الاستقصائات الكهروفسلجية تخطيط الاعصاب المحيطية الكهربائي وتخطيط العضلات الكهربائي،اذ شمل تخطيط الاعصاب المحيطية كفاءة الاعصاب الحسية بواسطة قياس الكمون الحسي، ارتفاع الجهد الحركي للاعصاب الحسية وسرعة التوصيل الحسي لكل من ( العصب الناصف"الوسطي"، العصب الزندي، والعصب الربلي)،اضافة الى دراسة وظائف الاعصاب الحركية من خلال دراسة الكمون الحركي القاصي ،سرعة التوصيل الحركي ،ارتفاع الجهد الحركي للاعصاب الحركية، قيمة الكمون الوسطى لموجة اف، التشتت الزمني وانعدام التوصيل الكهربائي لكل من الاعصاب التالية : - (العصب الناصف "الوسطي"، العصب الزندي ، العصب الشظوي المشترك والعصب الظنبوبي). في تخطيط العضلات الكهربائي تم فحص العضلات التالية : العضلة ذات الراسين والعضلة الاولى الظهرانية بين العظام للاطراف العليا اما بالنسبة للاطراف السفلى تم فحص العضلة المتسعة الانسية للفخذ ،العضلة الظنبوبية الامامية والعضلة الباسطة القصيرة للاصابع اذ تضمن الفحص استقصاء سعة الموجة، مدة كمون الفعل للوحدة الحركية والزيادة التدريجية في شدة المنعكس عند استمرار التنبيه. وكذلك تم فحص الاستجابة المتعاطفة للجلد للعصب الناصف "الوسطي"و العصب الشظوي المشترك الايمن. بينت الدراسة الكهروفسلجية لوظائف الاعصاب الحسية انخفاض ذو معنى في وظائف الاعصاب الحسية لدى المرضى الذين يعانون من الفشل الكلوي المزمن مقارنة بالاشخاص الاصحاء .اذ لوحظ زيادة في الكمون الحسي، انخفاض الجهد الحركي للعصب الحسي ونقصان في سرعة التوصيل الحسي . ولا يوجد فرق معنوي(p> 0.05) بين كلا النوعين من المرضى عدا فيما يتعلق بالكمون الحسي وسرعة التوصيل الحسي للعصب الناصف والزندي وكذلك العصب الربليز . اما فيما يخص تخطيط الاعصاب الحركية لكلا المجموعتين من المرضى بينت الدراسة انخفاضا ذو معنى لدى هؤلاء المرضى مقارنة بالاشخاص الاصحاء والتي شملت زيادة في الكمون الحركي القاصي وقيمة الكمون الوسطى لموجة اف ونقصان في سرعة التوصيل الحركي. اما الاستجابة المتعاطفة للجلد فقد اظهرت تدنيا معنويا(p< 0.05) لدى مرضى الفشل الكلوي المزمن في كلتا المجموعتين من المرضى عند مقارنتها مع الاشخاص الاصحاء . كما اظهرت الدراسة زيادة في التشتت الزمني في مرضى الفشل الكلوي في مجموعة قبل الديلزة وجدت في 39 عصب من اصل 204 عصب (19.3٪) وكانت اعلى نسبة في العصب الشظوي المشترك الايمن (28.57٪) ، في حين كانت اقل نسبة في العصب الزندي الايمن (12.5٪). وظهرت ايضزايادة التشتت الزمني في المرضى الخاضعين للديلزة وجد في 36 عصب من اصل 203 عصب (17.73٪) وكانت اعلى نسبة في العصب الشظوي المشترك الايمن(24.13٪)، في حين ان اقل نسبة مئوية في العصب المتوسط الايمن (12.5٪). كما اظهرت الدراسة ايضا وجود انعدام جزئي للتوصيل الكهربائي في المرضى الغير خاضعين للديلزة وكانت اعلى نسبة في العصب المتوسط الايمن (25٪)، في حين ان اقل نسبة شملت العصب الظنبوبي الايمن (11.11٪)، واظهرت الدراسة ان 38 عصب من اصل 204 عصب تم فحصها (18.62٪) تعاني من انعدام جزئي للتوصيل. اما المرضى الخاضعين للديلزة الغسيل الكلوي فقد كان هناك 34 عصب من اصل 203 عصب تم فحصها (16.74٪) يعاني من انعدام جزئي للتوصيل . وكانت اعلى نسبة هي في العصب المتوسط الايسر (25٪)، في حين ان النسبة المئوية الدنيا تشمل العصب الظنبوبي الايمن (7.69٪). نستخلص من هذه الدراسة ان تقييم الاعصاب الحسية اكثر اهمية من الحركية في تشخيص اعتلال الاعصاب الطرفية في وقت مبكر لدى مرضى الفشل الكلوي المزمن الغير خاضعين للديلزة كذلك الاستجابة المتعاطفة للجلد هي مفيدة جدا في الكشف عن اعتلال الالياف العصبية الصغيرة في المرضى المصابين بالاعتلال الكلوي المزمن ( قبل وبعد الغسيل الكلوي). وعلاوة على ذلك، فان مستوى الكرياتينين لديه علاقة عكسية واضحة مع كل معطيات دراسة توصيل العصب لدى المرضى الغيرخاضعين للديلزة (كلمزاادت نسبة الكرياتينين في مصل الدم يؤدي الى انخفاض قراات دراسة توصيل العصب). معظم مرضى الاعتلال الكلوي المزمن (الخاضعين وغير الخاضعين للديلزة) لديهم شذوذ في دراسة توصيل العصب في الاطراف، ولكن الاصابة في الاطراف السفلية هي اكثر من الاطراف العلوية.* اعتمد في تعريب المصطلحات العلمية على : 1. المعجم الطبي الموحد انكليزي عربي ،رئيس التجرير د.محمود الجليلي الطبعة الثانية،1978. 2. الموجز المصور لفحص الجهاز العصبي. ترجمة د.عبد الهادي الخليلي - بغداد - 1992 د.موريس فان الن. د.روبرت روزنسكي. | The Chronic Kidney Disease (CKD) is a purposeful identification portrayed by an irretrievable and step by step advancing decrease in glomerular filtration rate (GFR). Furthermore, it is intricate by a rising incapability to preserve common stages of protein metabolism products (such as urea, creatinine, standard blood pressure, hematocrit, sodium, water, calcium phosphate homeostasis, potassium and acid base balance). A further frequent involvement is the Peripheral neuropathy of Chronic kidney disease. Nevertheless, patients who suffer CKD are further exposed to increase peripheral polyneuropathy. Neurological impediments that is subsidiary to the uremic status, participate fundamentally to the sickness and death to patients who suffer renal failure. The occurrence of peripheral neuropathy stays extreme in advanced renal dysfunction Electrophysiological study (nerve conduction study NCS and electromyography EMG) is useful adjunct test to the medical history and clinical examination in the diagnosis of such complication, early detection and differentiation from an axonal type of peripheral neuropathy. The aims of this study are designed to : (1) Evaluate the function of electrophysiological study (NCS and EMG) in diagnosing the neuropathy for patients who suffer CKD. (2) Decide and rule out the most necessary parameters of NCS and EMG, to be performed as preliminary test in the diagnosis of small fiber neuropathy. (3) Correlation between the severity of the peripheral neuropathy and the level of serum creatinine.Three groups of subjects included in this study : Adult patients of chronic kidney disease not on dialysis yet, Patients on hemodialysis and control subjects. The present study was carried out at the neurophysiology unit in Al - Yarmouk Teaching Hospital, in the a period from June/2016 to September /2017.The control group consists of forty two (42) healthy volunteers (22 males and 20 females). The age of this group ranged between 20 to 75 years with a mean 52.48 ±10.63 years. Males are 22 - 75 years with a mean of age 53.54± 14.57 years, and 20 - 74 years for females with a mean of age 51.42±11.91 years. Eighty patients included in this study had chronic kidney disease. They consist of two groups : A - Forty (40) patients with chronic kidney disease on customary attend the Kidney and Dialysis clinic not on dialysis with steadily high serum creatinine (> 2.5 mg/dl of) either sex (22 males and 18 females), their ages ranging between 35 and 72 years with a mean 55.35 ± 9.05 years. The duration of CKD is ranged between 2 – 10 years, with mean 8.72 ± 3.65 yearsB - Forty (40) patients with chronic kidney disease on regular hemodialysis (21 males and 19 females), their ages ranging between 43 to 70 years with a mean 56.98 ±7.40 years. The duration of CKD is ranged between 7 - 20 years with mean 13.87 ± 5.41 years. All of 122 subjects (patients and control) that were included in this study were informed about the aim and technique of the study and their acceptance was taken.Each subject was submitted to medical history and electrophysiological tests (NCS and EMG) of the two limbs i.e. the upper and the lower. These tests include : 1 - Sensory nerve conducting study (SNCS) for Median, Ulnerand Sural nerves, in which, distal sensory latency, sensory nerve action potential (SNAP) amplitude and sensory nerve conduction velocity (SNCV) are performed. 2 - Motor nerve conducting study (MNCS) for Median, Ulnar, Fibular (Common peroneal), and tibial nerves , which includes measurement of distal motor latency (DML), compound muscle action potential (CMAP) amplitude, Motor nerve conduction velocity (MNCV), Mean F - wave latency, Terminal latency index (TLI), Temporal dispersion (TD%) and Conduction block (CB%).3 - Sympathetic skin response (SSR) of right median and right fibular nerves. 4 - Needle EMG for proximal and distal muscles like : biceps, and 1st dorsal interosseous muscles for upper limb and vastusmedialus, tibialis anterior and Extensor digitorium brevis for lower limb, in which insertional activity, spontaneous activity, motor unit action potential (duration, amplitude and polyphasia%) and recruitment pattern are considered. The results of this study revealed that sensory NCS was considerably altered in all kinds of CKD patients as weigh against the healthy control subjects in the form of prolonged distal sensory latency, decreased SNAP amplitude and slowing of SNCV. However, there were not statistically significant differences (P>0.05) between pre dialysis and post dialysis Peripheral Neuropathy except in the SNAP amplitude of right and left median and right ulnar and sural sensory nerves whereas the differences are statistically significant (p<0.05).As for the study of motor nerve conduction, theprolonged DML, slowing of conduction velocity and prolonged mean F - wave latency are perceived in both groups of patients when compared with those of healthy control subjects. However, the differences are also statistically significant (P<0.01) in the TLI of right median and ulnar nerves, distal CMAP amplitude of the right ulnar nerve, distal motor latency (DML) of left median and right ulnar nerves, MNCV (m/sec.) of left median nerve and prolongation or mean F - wave latency of left median, common peroneal and right tibial nerves (P<0.05). Also Sympathetic Skin Response significantly (P<0.01) changed detected in both groups of patients when compared with those of healthy control.Moreover, in both patients groups, abnormal TD% are observed increased temporal dispersion in CKD pre dialysis patients is found in 39 out of 204 nerves (19.3%) However the higher percentage of abnormal TD% is in the right common peroneal nerve (28.57%), while the lower percentage is in the right ulnar nerve (12.5%).Increased temporal dispersion in CKD post dialysis patients is found in 36 out of 203 nerves (17.73%) However the higher percentage of abnormal TD% is in the right common peroneal nerve (24.13%), while the lower percentage is in the right median nerve (12.5%).In both patients groups, abnormal CB% are observed that the higher percentage of conduction block (CB%) in motor nerves of CKD pre dialysis patient involves right median nerve (25%), while the lower percentage involves right tibial nerve (11.11%).Whereas there are 38 out of 204 examined nerve with conduction block (18.62%).And also show that the higher percentage of conduction block (CB%) in motor nerves of CKD post dialysis patient involves left median nerve (25%), while the lower percentage involves right tibial nerve (7.69%). Whereas there are 34 out of 203 examined nerve with conduction block (16.74%).In conclusion, this study reveals Sensory NCS is more useful and helpful than the motor NCS in the diagnosis of early peripheral neuropathy in CKD pre dialysis also Sympathetic skin response (SSR) abnormality are very useful in the detection of small fiber neuropathy in CKD patients (pre and post dialysis).Moreover, the serum creatinine level have strong correlation with NCS parameters in CKD pre dialysis patients (when increase level of s.cr lead to decrease the NCS parameters).Most CKD patients had abnormal NCS parameters in both limbs, but the abnormalities in the lower limbs are more than the upper limbs

تقييم تاثيرات المتفورمين والستاقلبتين على تفاعلية الاوعية الدموية الدماغية لدى مرضى السكري من النوع الثاني بواسطة دوبلر الدماغ : دراسة سريرية مقارنة == Evaluation The Effects of Metformin and Sitagliptin on Cerebral Vasoreactivity in Diabetic Patients (Type 2) By Transcranial Doppler : A Comparative Clinical Study

Author name: احمد محمد حسن المظفر
Supervisor name: حيدر كاظم حسون | محمد رشاد ابراهيم
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: ان مرض السكري من النوع الثاني يزيد من خطر الاصابة بالجلطة الدماغية ب (3 - 6) اضعاف وذلك بسبب اضطراب وظيفة النسيج الطلائي للاوعية الدموية وما ينتج عنه من تصلب مبكر لشرايين الجسم بشكل عام ومنها شرايين الدماغ. ان الكشف والعلاج المبكرين لذلك يقلل من خطر الجلطة الدماغية. ان جهاز دوبلر الدماغ هو جهاز لفحص شرايين الدماغ ومن الممكن تطبيق بعض الاختبارات الفسلجية لفحص مرونة وتفاعلية شرايين الدماغ.لقد اثبتت الدراسات السابقة ان ضعف تفاعلية شرايين الدماغ يزيد من احتمالية الاصابة بالجلطة الدماغية ولذلك من الممكن تقييم تاثيرات الادوية المستخدمة لعلاج داء السكري من النوع الثاني على تقليل خطر الاصابة بالجلطة الدماغية.هدف الدراسة : لقد اجريت هذه الدراسة من اجل تقييم تاثيرات عقاري المتفورمين والسيتاقلبتين والجمع بينهما على تفاعلية الاوعية الدموية الدماغية في مرضى السكري من النوع الثاني والمشخص حديثا باستخدام جهاز دوبلر الدماغ.طريقة العمل : اجريت هذه الدراسة في عامي (2015 - 2016) وقد شارك في الدراسة 182مريضا جميعهم من المصابين بداء السكري من النوع الثاني والمشخص لديهم حديثا. تم استبعاد مائة وثلاث مرضى اما الباقين تم تقسيمهم الى اربعة مجاميع. المجموعة الاولى هي مجموعة الضبط (وشملت 20 مريض) حيث تم علاجهم بواسطة ضبط النظام الغذائي وممارسة الرياضة فقط اما المجموعة الثانية فقد تم علاجهم بعقار المتفورمين بجرعة قدرها خمسمائة ملغرام مرتين يوميا لمدة ثلاثة اشهر اضافة الى ضبط النظام الغذائي وممارسة الرياضة للاقلال من وزن الجسم ووشملت 20 مريض . اما المجموعة الثالثة فقد تم علاجهم بعقار السيتاقلبتين عن طريق الفم مرتين يوميا لمدة ثلاثة اشهر وبجرعة قدرها خمسون ملغرام اضافة الى ضبط النظام الغذائي وممارسة الرياضة وشملت 19 مريض. اما المجموعة الرابعة فقد عولجوا بالسيتاقلبتين والمتفورمين مجتمعين مع ضبط النظام الغذائي وممارسة الرياضة ايضا وشملت 20 مريض. وقد اخذت موافقة جميع المرضى شفهيا قبل المشاركة. بعد ذلك تم اجراء الفحوصات المختبرية وفحص دوبلر الدماغ للشريان المخي الاوسط وتم تسجيل سرعة جريان الدم الانقباضية والانبساطية والوسطى وتم تسجيل معامل مقاومة الشريان ومعامل القدرة الانبساطية والانقباضية للشريان. ثم تم فحص تفاعلية الشريان المخي الاوسط بواسطة قياس مؤشر كتم النفس ومؤشر مدى التوسع الكامل للاوعية الدموية ومقياس فرط جريان الدم لكل مريض قبل وبعد ثلاثة اشهر من العلاج.النتائج : سجلت جميع مجاميع الدراسة زيادة ذات دلالة احصائية في مؤشر كتم النفس الا ان اكثرها قوة كان في المجموعة الرابعة (مجموعة الجمع) حيث كانت الزيادة من 0.64±0.029)) الى (0.87±0.035) مقارنة بالمجموعة الاولى (مجموعة الضبط) حيث كانت الزيادة من (0.67±0.027 ) الى (029 ( 0.72±0.اما بالنسبة الى مؤشر مدى التوسع الكامل للاوعية الدموية , لم تظهر جميع مجموعات الدراسة زيادة ذات دلالة احصائية في هذا المؤشر الا المجموعة الرابعة (مجموعة الجمع) حيث اظهرت هذه المجموعة زيادة ذات دلالة احصائية في هذا المؤشربعد العلاج من 30.3±1.18%)) الى (35.75±2.09%) .وفيما يتعلق بمقياس فرط جريان الدم , لم تظهر المجموعة الاولى (مجموعة الضبط) فرق ذو دلالة احصائية بعد العلاج بينما اظهرت المجموعتين الثانية والثالثة (مجموعتي المتفورمين والسيتاقلبتين) زيادة ذات دلالة احصائية وقد اظهرت المجموعة الرابعة (مجموعة الجمع) الزيادة الاكبر في هذا المقياس من بين جميع مجموعات الدراسة من ) 1.24±0.057) الى ( 1.59±0.06) .الاستنتاجات : 1. ان ادوية الميتفورمين والسيتاقلبتين منفردة زادت بصورة ملحوظة من تدفق الدم الانبساطي من للشريان المخي الاوسط وتخفيض RI وPI هذا التاثير اكثر وضوحا عندما يتم اعطاء كل من الدوائين معا مما يزيد من مرونة شرايين الدماغ.2. ان ممارسة الرياضة وبرنامج الحمية الغذائية زاد من مؤشر كتم التنفس كما الميتفورمين وعلاج السيتاقلبتين. ومع ذلك، فان التاثير اكثر وضوحا مع مزيج من الميتفورمين والسيتاقلبتين.3. ان ممارسة الرياضة وبرنامج الحمية الغذائية ،وادوية الميتفورمين والسيتاقلبتين لا يزيد مقياس مدى التوسع الكامل للاوعية الدموية او مقياس فرط جريان الدم في المرضى الذين يعانون من النوع 2 من داء السكري الذي اكتشف لديهم حديثا. بينما الجمع بين علاج الميتفورمين والسيتاقلبتين له تاثير اكثر اهمية في مقياس مدى التوسع الكامل للاوعية الدموية او مقياس فرط جريان الدم.4. وقد وجدت الدراسة وجود تناسب عكسي ذا دلالة احصائية واضحة بين نسبة السكر في الدم ومعدل السكر التراكمي من جهة ومقاييس تفاعلية الاوعية الدموية الدماغية (مؤشر كتم التنفس ومؤشر فرط جريان الدم ) من جهة اخرى.5. ان الجمع بين عقار المتفورمين والسيتاقلبتين في علاج مرضى السكري من النوع الثاني يقلل خطر الاصابة بالجلطة الدماغية من خلال زيادة كل مقاييس تفاعلية الاوعية الدموية الدماغية (مؤشر كتم النفس ومقياس مدى التوسع الكامل للاوعية الدموية ومؤشر فرط جريان الدم ) | Diabetes mellitus type 2 increases the risk of stroke 3 folds. It do so by inducing endothelial injury and accelerated atherosclerosis. Early diagnosis and treatment lower the risk of stroke. Transcranial Doppler examination applied with certain physiological tests can be used to test vasoreactivity of intracranial arteries. Impaired cerebral vasoreactivity can predict the risk of stroke. Therefore , this diagnostic test can be used for evaluation the effect of oral hypoglycemic drugs on risk of stroke. This study was conducted for evaluation and comparison of the effects of metformin, sitagliptin and their combination on cerebrovascular reactivity in patients with newly diagnosed type 2 diabetic patients. A total of 79 patients were involved in this study. They are all with newly diagnosed type 2 diabetes mellitus. They were divided into four groups; control, metformin, sitagliptin and combination. A verbal consent were taken from each participant. Control group includes twenty patients and was treated with dietary control and regular exercise. Metformin group includes twenty patients and was treated with metformin 500mg twice daily plus dietary control and exercise. Sitagliptin group includes nineteen patients and was treated with sitagliptin 50mg twice daily plus dietary control and regular exercise. Finally, combination group includes twenty patients and was treated with dietary control and regular exercise plus metformin 500mg with 50mg of sitagliptin twice daily. Treatment duration was three months. Biochemical tests were done before and after treatment. Measurement of hemodynamic parameters including systolic, diastolic and mean velocities with pulsatility and resistive indices was done by transcranial Doppler before and after treatment. Vasoreactivity measures which include breath - holding index, FRVD and transient hyperemic response rate ,all were recorded for each patient before and after treatment. In control group, there was significant statistical increase in breath - holding index from (p=0.008). In metformin group, breath - holding index also significantly increased (p<0.000). In sitagliptin group, breath holding index showed significant increase (p<0.000). Finally, combination group showed highest level after treatment among study group (p<0.000). Regarding FRVD; control group revealed insignificant statistical difference after treatment (p=0.702). Metformin group showed increase in FRVD it was statistically insignificant (p=0.071). Sitagliptin group exhibited non - significant statistical difference in FRVD (p=0.701). On the other hand, combination group revealed significant statistical increase in FRVD (p=0.001). In control group, THRR increased after treatment but it was insignificant change (p=0.209). Metformin group showed increase in THRR which was significant change (p=0.019). Sitagliptin group showed significant increase in THRR (p<0.000). Finally, combination group showed significant statistical increase in THRR (p=0.0003) which was the highest value among groups after treatment. From the results obtained in this study we can conclude that treatment with metformin plus sitagliptin increase all parameters of cerebral vasoreactivity in this study. Furthermore, decrease in fasting blood sugar and HbA1c correlates inversely with increase in breath - holding index after treatment

تاثير عقار السيتاقلبتين على الصدفية المزمنة من النوع اللويحي لدى مرضى السكري في العراق == Effect of Sitagliptin on chronic plaque psoriasis of diabetic patients in Iraq

Author name: سرمد نوري الدجيلي
Supervisor name: نصير عبد الامير الحرجان | محسن عبد الحسين الظالمي
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: مرض الصدفية يعتبر من الامراض المزمنة النظامية المعقدة والمحفزة مناعيا بواسطة خلية المناعة من نوع ت ويتميز بلويحات حمراء مقشرة من الجلد والمفاصل. وقد حفز وسطاء الالتهابات 17IL - ,TNF - α وIL - 6 وربما عملية الجهد الناكسدي على انقسام خلايا بشرة الجلد وتمايزها مما ادى الى ظهور الصفات المميزة للمرض. مرضى الصدفية مع النوع اللويحي لا سيما مع ارتفاع مؤشر كتلة الجسم لديهم خطر الاصابة بمرض السكري من النوع الثاني وارتفاع الدهون. العلاجات الحديثة البيولوجية للمرض مثل مضادات α - TNF ومضاد ustekinumab) IL - 17) قد اسفرت عن نتائج افضل من العلاجات التقليدية غير ان استخدامها قد انحسر لعدد قليل من المرضى لما لها من اثار جانبية خطيرة. السيتاقلبتين يعتبر من الكابحات لانزيم الدايبيبتديل ببتايتيز4 وله تاثير مضاد للالتهابات للمرضى الذين يعانون من داء السكري من النوع الثاني وبدون اي تاثيرات جانبية . ولذلك فان الهدف من الدراسة الحالية هو تقييم اثر السيتاقلبتين على درجة باسي عبر التداخل مع معلمات متلازمة الايض, وسطاء الالتهابات وعلامات الاجهاد التاكسدي لمرضى الصدفية المصابين بالسكري من النوع الثاني.المرضى وطرق العمل : لقد اجريت الدراسة على 48 مريضا مصابا بمرض الصدفية يعانون من داء السكري وتم تقسيمهم الى مجموعتين : مجموعة البلاسيبو (ن = ٢٤) : اعطيت١٠٠ملغم من كبسولة البلاسيبو مرة واحدة يوميا بالاضافة الى مراقبة النظام الغذائي وممارسة التمارين الرياضية لمدة ١٢ اسبوع. مجموعة السيتاقلبتين (ن = ٢٤) : اعطيت قرص السيتاقلبتين ١٠٠ملغم مرة واحدة يوميا بالاضافة الى مراقبة النظام الغذائي وممارسة التمارين الرياضية لمدة ١٢ اسبوع. وقد تم الحصول على عينات الدم من المرضى في كلا المجموعتين قبل وبعد استخدام ١٢ اسبوعا من العلاج لقياس تركيز المصل من السكر بعد الصيام ، HbA1c، الدهون الثلاثية، الكولسترول، البروتين الدهني منخفض الكثافة ،البروتين الدهني منخفض الكثافة جدا ،البروتين الدهني عالي الكثافة ، α TNF - ، IL - 17، IL - 6، IL - 10، MDA وGSH، ودراسة العلاقة مع درجة باسي بعد ١٢ اسبوعا من العلاج. تم اجراء الخزعات من نوع لكمة مع قطر ۵ملم سمك لكلا المجموعتين قبل وبعد ١٢ اسبوعا من العلاج وارسالها للفحص النسيجي.النتائج : مقارنة مع خط الاساس في مجموعة السيتاقلبتين ومجموعة البلاسيبو بعد ١٢ اسبوعا من العلاج مستوى السكر بعد الصيام، نسبة HbA1c، الدهون الثلاثية، الكولسترول، البروتين الدهني منخفض الكثافة ، البروتين الدهني منخفض الكثافة جدا، TNF - α ، IL - 17 وIL - 6في مصل الدم اظهرت انخفاضا معنويا (0.05 >P) بعد ١٢ اسبوعا من العلاج بالسيتاقلبتين وارتباطا ملحوظا موجبا مع درجة باسي (0.05 >P) . في المقابل مستوى البروتين الدهني عالي الكثافة، IL10 وGSHفي الدم اظهرت ارتفاعا معنويا (0.05 >P) بعد ١٢ اسبوعا من العلاج بالسيتاقلبتين وارتباط سلبي ملحوظ مع درجة باسي (0.05 >P) . وكشف فحص الانسجة تغييرات معنوية (0.05 >P) بعد ١٢ اسبوعا من العلاج بالسيتاقلبتين في الصفات النسيجية للبشرة والخلايا اللمفاوية المتسللة لادمة الجلد بالمقارنة مع قبل العلاج لمجموعة السيتاقلبتين وبعد١٢ اسبوعا من العلاج لمجموعة البلاسيبو ولا يوجد اي تاثير معنوي على الاوعية الدموية لادمة الجلد.الاستنتاج : كشفت الدراسة ان السيتاقلبتين قد خفض درجة باسي دون غياب كامل للويحات الصدفية من خلال تحسن ارتفاع السكر في الدم، الدهون، وسطاء الالتهابات او السيتوكينات ومسارات الاجهاد التاكسدي مع تاكيد النتائج السريرية والمناعية من حلال التحسن الملحوظ في التغييرات النسيجية المرضية . | Psoriasis represents a complex chronic systemic T cell immune - mediated inflammatory disease characterized by erythematous, scaly plaques of skin and joints. Inflammatory mediators such as IL - 17, IL - 6, TNF - α and possibly oxidative stress process have stimulated abnormal proliferation of keratinocytes and differentiation resulting in characteristic appearance of psoriasis. Psoriatic patients with plaque psoriasis particularly those with high body mass index have increasing risk of developing a diabetes mellitus type 2 (DM2) and hyperlipidemia. Advance treatment of psoriasis with biological agents such as TNF - antagonists and IL - 17 antagonist like ustekinumab have been tried with a better result than traditional treatment. However their use have been limited in some patients, because of severe side effects. Sitagliptin is a dipeptidyl peptidase - IV (DPP - IV) inhibitor exerts anti - inflammatory effect when used in patients with type 2 diabetes without any reported severe side effects. Therefore the objective of current study was to assess the effect of Sitagliptin on psoriasis area and severity index (PASI) score in psoriatic patients with diabetes via interfering with metabolic syndrome parameters, inflammatory mediators and oxidative stress markers.Patients and Methods The study was conducted on 48 diabetic patients with moderate to severe plaque psoriasis who were divided into two groups : Placebo group (n=24) patients were administered placebo once daily plus dietary control and exercise for 12 weeks ; Sitagliptin group (n=24) patients were administered Sitagliptin tablet 100mg once a day plus dietary control and exercise for 12 weeks. PASI score for all patients was assessed before and after 12 weeks of treatment. The fasting blood samples were obtained from the patients in both groups at baseline and after 12 week of therapy used to measure the concentration of serum fasting blood sugar (FBS), HbA1c, triglyceride(TG), cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), tumor necrosis factor alpha (TNF - α), intreleukin - 17 (IL - 17), intreleukin - 6 (IL - 6), intreleukin - 10 (IL - 10), malondialdehyde (MDA) and reduced glutathione (GSH), and to determine their correlation with PASI score after 12 weeks of treatment. Punch biopsies with size of 5mm diameter were performed for both groups at baseline and after 12 week of treatment and sent for histopathological examination and psoriasis histopathological score (PHS) was measured for patients in both groups at baseline and after 12 week of treatment. Results : Compared with baseline in Sitagliptin group and placebo group after 12week, the level of PASI score was significantly reduced (P < 0.05) after 12 weeks of Sitagliptin treatment. The level of FBS, HbA1c, TG, cholesterol, LDL, VLDL, TNF - α, IL - 17, IL - 6 and MDA were significantly reduced (P < 0.05) after 12 weeks of Sitagliptin treatment when compared with baseline in Sitagliptin group and placebo - treated group after 12 week and positively correlated (P < 0.05) with PASI score. In contrast the level of HDL, IL10 and GSH were significantly increased (P < 0.05) after 12 weeks of Sitagliptin treatment in comparison to baseline in sitagliptin group and with that of placebo - treated group after 12week and negatively correlated with PASI score (P < 0.05). PHS was significantly reduced (P < 0.05) after 12 weeks in comparison to baseline in Sitagliptin - treated group and with that of placebo - treated group after 12 week. Histopathological examination also revealed a significant improvement (P < 0.05) in epidermal histological features and dermal lymphocytic infiltration with no effect on dermal blood vessels.Conclusion The present study revealed that Sitagliptin reduce PASI score without complete absence of psoriatic plaques via an improvement in hyperglycemia, hyperlipidemia, inflammatory mediators or cytokines and oxidative stress markers with confirmation of our clinical and immunological results by significant improvement in PHS.

تاثير الاجسام المضادة لل انفلياكسيمب وعلاقتها مع مستضدات الكريات البيض البشرية - النوع الثاني - DRB1 في المرضى العراقيين المصابين بالتهاب المفاصل الرثوي == Influence of Anti - infliximab Antibodies and its Association with HLA - class II - DRB1 alleles in Iraqi patients with Rheumatoid arthritis

Author name: محمد احمد الكرخي
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: التهاب المفاصل الرثوي هو مرض مزمن ذاتي المناعة يصيب عدة اعضاء من الجسم ومن ضمنها المفاصل ويمكن ان يؤدي الى تدمير المفاصل والعجز الشديد اذا لم يعالج في وقت مبكر. المضادات لعامل نخر الورم عامل الفا (TNFα) مثل اينفلياكسيمب (INF) وهو من الاجسام المضادة وحيدة النسيلة خيالية ادخلت في عام 2010 من قبل وزارة الصحة كعوامل فعالة للغاية لعلاج المرضى العراقيين المصابين بالتهاب المفاصل الرثوي الحاد متوسط الشدة او الشديد الذي لا يستجيب للعلاج التقليدي.اينفلياكسيمب يمكن ان يحفز على تكوين الاجسام المضادة لعقار اينفلياكسيمب (ATIS)، ولكن هذه الاجسام المضادة من الممكن ان تؤدي الى نقص فعالية الاينفلياكسيمب او تاثيرات جانبية سلبية.وفقا لمعلوماتنا المتاحة انه لا توجد اية معلومات عن هذه الاجسام المضادة لل انفلياكسيمب وارتباطها مع النتائج السريرية، العلامات المصلية ومستضدات HLA - classІІ - DRB1 في المرضى العراقيين المصابين بالتهاب المفاصل الرثوي.وقد اجريت هذه الدراسة لاعطاء فكرة واضحة عن تكون ودور هذه الاجسام المضادة في الاستجابة السريرية للمرضى لعقار الاينفلياكسيمب وكذلك توضيح تاثير مستضداتHLA - classІІ - DRB1 على القابلية المناعية لعقار الانفلياكسيمب في تكوين هذه المضادات.المرضى والطرق : شملت الدراسة 100 شخص. حيث تضمنت ما مجموعه 50 مريضا الذين توافقوا مع المعايير المنقحة لمرض التهاب المفاصل الرثوي (الكلية الامريكية لامراض الروماتيزم 1987 و2010) في المواءمة مع 50 مجموعة السيطرة (25 اشخاص اصحاء و25 مرضى مصابين بالتهاب المفاصل الرثوي لم يعالجوا بعقار اينفلياكسيمب). وقد اجريت هذه الدراسة خلال الفترة من بداية شهراذار 2014 حتى نهاية شهر ايلول 2014 في وحدة العلاج البيولوجي لقسم امراض الروماتيزم / مستشفى بغداد التعليمي، مختبر الدراسات العليا / فرع الاحياء المجهرية / كلية الطب - جامعة بغداد، المختبرات التعليمية / مدينة الطب، وحدة زرع الكلى / مستشفى الكرامة التعليمي ومركز التطابق النسيجي البحثي / كلية طب الكندي / جامعة بغداد. تم جمع عينات الدم من مجموعة السيطرة والمرضى الذين يعانون من التهاب المفاصل الرثوي في والذين تمت معالجتهم بعقار الانفلياكسيمب لاكثر من ثلاثة اشهر لغرض تقييم الاجسام المضادة لعقار الاينفلياكسيمب،مضادات - CCP، بروتين سي التفاعلي والتنميط الجيني HLA - DRB1.المرضى الذين لم تتكون لديهم الاجسام المضادة لل انفلياكسيمب فتمت متابعتهم لمدة 6 اشهر واعادة فحصهم للتحقق من وجود الاجسام المضادة لل انفلياكسيمب,anti - CCP وبروتين سي التفاعلي.تم الكشف عن الاجسام المضادة لل انفلياكسيمب والاجسام المضادة لل CCP بواسطة جهاز فحص الانزيم الرابط المناعي (ELISA) ,اما بروتين سي التفاعلي(CRP) فتمت قياسته بواسطة جهازNephelometer في حين تم اجراء تحليل التنميط الجيني النوع الثاني DRB1 بواسطة بادئات محددة تفاعل البلمرة المتسلسل (PCR - SSO) لكل من المرضى ومجموعة السيطرة الاصحاء.النتائج : من اصل 50 مريض في الدراسة الحالية، كان متوسط العمر (45.24 ± 9.15سنة) وكانت الاناث اكثر من الذكور (اناث : ذكور 3 : 1).تم اكتشاف الاجسام المضادة لل انفلياكسيمب في خمسة وثلاثون مريض(70٪) من مرضى التهاب المفاصل الرثوي بعد فترة ثلاثة اشهر من العلاج بهذا الدواء, بينما لم تتكون هذه المضادات للعقار في 15 (30٪) منهم.في حين لم يتم الكشف عن اية اجسام مضادة في مجموعة السيطرة (الاصحاء ووالسيطرة المرضية)، كان الفرق على درجة عالية وذات دلالة احصائية (قيمةp = 0.000001)، ومع حساسية وخصوصية (70٪، 100٪) على التوالي. في نفس المدة من العلاج تم الكشف عن الاجسام المضادة لل - CCP في 47 (94٪) من المرضى بينما لم يتم اكتشاف هذه الاجسام المضادة في في 3 (6.0٪)منهم، في حين تم الكشف عن هذه الاجسام المضادة في 22 (88٪) من مجموعة السيطرة ولم يتم اكتشافها في 3 (12٪) منهم.بينما لم يتم اكتشاف اية اجسام مضادة لل - CCPفي مجموعة الاصحاء ,وكان الفرق معتد للغاية وذات دلالة احصائية (قيمة p=0.0005) مع حساسية وخصوصية (94٪، 100٪) على التوالي.تم توثيق علاقة قوية بين نشوء الاجسام المضادة للانفلياكسيمب والاجسام المضادة لل - CCP (قيمة p = 0.006).وفيما يتعلق بفحص بروتين سي التفاعلي، تم الكشف عنه في 26 (52٪) من مجموعةالمرضى بينما لم يتم الكشف عنه في 24 (48٪) منهم، كما انه كان ايجابيا في كل مجموعة السيطرة المرضية, بينما في مجموعة السيطرة للاصحاء، كان ايجابيا في 10 (40٪) وسلبيا في 15 (60٪) وكان الفرق ذات دلالة احصائية عالية (قيمة p= 0.000014) مع تدني في الحساسية والخصوصية (52٪، 60٪) على التوالي.ولم تسجل الدراسة الحالية اي ارتباط ذو قيمة بين نتيجة بروتين سي التفاعلي وتكون اضداد اينفلياكسيمب (قيمةp = 0.084).بالنسبة لمجموع المرضى الذين لم يتم اكتشاف اضداد ال انفلياكسيمب لديهم والبالغ عددهم 15 مريض فتمت متابعتهم بعد 6 اشهر من فترة العلاج بال انفلياكسيمب لغرض التحقق من احتمالية تكون الاجسام المضادة لل انفلياكسيمب ,اضافة الى اعادة فحص اضداد - CCPو بروتين سي التفاعلي ,اظهرت النتائج عن عدم وجود فرق احصائي بين نتائج الفحص بعد 3 اشهر وونتائج الفحص بعد 6 اشهر وكانت قيمة - p((0.067، 0.067 و1.0) على التوالي.وعلاوة على ذلك، كشفت الدراسة وجود علاقة ايجابية بين HLA - DRB1 * 04 (DR4) اليل مع 35 مريض مصاب بالتهاب المفاصل الرثوي تمت علاجهم بال اينفلياكسيمب حيث تمت مقارنتهم مع 25شخص من مجموعة السيطرة للاصحاء وكان الفرق ذات دلالة احصائية (قيمة p = 0.001).واخيرا كشفت الدراسة الحالية عن عدم وجود اي ارتباط بين HLA - DRB1 وتكون الاجسام المضادة لل اينفلياكسيمب في المرضى الذين يعانون من التهاب المفاصل الرثوي (قيمة p = 0.0914).الاستنتاجات : اظهرت الدراسة الحالية على ان ما يقرب من ثلاثة ارباع المرضى العراقيين المصابين بمرض التهاب المفاصل الرثوي تتكون لديهم الاجسام المضادة لل ااينفلياكسيمب بعد ثلاثة اشهر من العلاج مع هذا العامل البيولوجي مع عدم وجود اي ارتباط للعامل الوراثي (اليل DRB1 - HLAالنوع الثاني) مع تكونها، كما اثبتت الدراسة وجود ارتباط وثيق بين العامل الجيني الاليل( HLA - DRB1 * 04 (DR4مع نشوء مرض التهاب المفاصل الرثوي في المرضى العراقيين. | Rheumatoid arthritis is a systemic chronic autoimmune inflammatory polyarthritis leads to joints destruction and severe disability if not treated early. Tumor necrosis factor alpha antagonists such as infliximabwhich is a chimeric monoclonal antibody have been introduced and approved in 2010 by Ministry of Health as highly effective agents for the treatment of Iraqi patients with moderate to severe Rheumatoid arthritis that not responds to conventional treatment.Infliximab can induce formation of anti - infliximab antibodies, however, these antibodies may lead to lack of efficacy to Infliximab or adverse reactions.According to our knowlege no information is available about these antibodies to infliximab and their association with clinical findings , serological markers and HLA - class ІІ - DRB1 antigens in iraqi patients with Rheumatoid arthritis.The present study was conducted to give a clear idea about the development and the role of these antibodies in clinical response of patients to infliximab and demonstration the effect of HLA - class ІІ - DRB1 antigens on the immungenicity of infliximab. Patients and Methods : The study covered 100 subjects. It included a total of 50 patients who met the revised criteria for RA (the American College of Rheumatology 1987 and 2010) in aligment with the 50 control group(25 healthy individuals and 25 RA patients not treated by infliximab). This study was carried out during the beginning of March 2014 till the end of September 2014 in the Biological Therapy Unit of the Deparment of Rheumatology/Baghdad Teaching Hospital, the laboratory for Higher Studies/Microbiology Deparment/Baghdad College of Medicine, the Teaching Laboratories/Medical City, the Kidney Transplant Unit/ Al - Karama Teaching Hospital and HLA Research Center /Al - kindy College of Medicine/Baghdad University. Blood samples were collected from control group and patients with RA on infliximab therapy for more than three months duration for assesment of antibodies to infliximab , anti - Cyclic Citrullinated Peptide(anti - CCP) ,C - reactive Protein(CRP) and for Human Leukocyte Antigen - DRB1(HLA - DRB1) genotyping.The patients with negative anti - infliximab antibodies were follow up and reinvestigated after 6 month of infliximab therapy for anti - infliximab antibodies, anti - cyclic citrullinated peptide and C - reactive Protein.Anti - bodies to infliximab and anti - Cyclic Citrullinated Peptide antibodies were detected by Enzyme Link Immunosorbent Assay (ELISA), the C - reactive protien (CRP) was measured by Nephlometer . whereas the genotyping analysis for HLA - class ІІ - DRB1 had been done by polymerase chain reaction - sequence specific primers (PCR - SSO) for both patients and healthy control group.Results : Out of 50 case study group included in the current study, their mean age was (45.24± 9.15) the females were predminance than males in a ratio (F : M 3 : 1).Thirty five (70%) of RA patients developed antibodies against infliximab after three months of treatment with this drug ,15 (30%) of them were negative for these antibodies, while no anti - inflixmab antibodies were detected in control groups( (healthy and case control) ,the difference was a highly statistically significant(P - value=0.000001), and with sensitivity and specificity (70 %,100%) respectively.At the same duration of treatment the anti - CCP was detected in 47 (94%) patients and negative in 3 (6.0%), while in case control was postive in 22 ( 88%) and negative in 3(12%), and was not detected in all healthy control group,the difference was highly satistically siginficant ( p - value =0.0005) with sensitivity and specificity (94%,100%) repectively.A strong correlation was reported between the development of anti - infliximab and anti - CCP antibodies (p - value=0.006).Regarding the CRP test ,it was positive in 26 (52%) patients and negative in 24 (48%), also it was positive in all case control group while in healthy control group ,it was reported positive in 10(40%) and negative in 15(60%) with highly statistically significance(p - value=0.000014) with low sensitivity and specificity (52%, 60%) respectively.No significant correlation was reported between CRP and development of anti - infliximab antibodies (p - value=.084).Fifteen patients group with negative anti - infliximab antibodies were follow up and re - investigated after six months of treatment with infliximab for anti - infliximab ,anti - CCP antibodies and CRP, the results showed no statistical difference of these test after three and six month of infliximab treatment with p - value (0.067, 0.067 and 1.0) respectively.The study revealed a positive association of HLA - DRB1*04(DR4) allele with 35 patients with RA on infliximab therapy whom compared with 25 healthy control and the difference was statistically significant (p - value=0.001).There was no association found between the HLA - DRB1 and development of antibodies to infliximab in patients with RA (p - value=0.0914).Conclusions : The current thesis showed that approximately three quarter of Iraqi patients with RA developed antibodies against infliximab after three months of treatment with this biological agent and without association of genetic factor( HLA - class ІІ - DRB1 alleles) with their development ,also the study suggested an association of susceptibility of RA in Iraqi patients with HLA - DRB1*04(DR4).

دور Foxp جين والاحماض النووية الرايبوية متناهية الصغر ,145 ,146a ,20aفي عينة من المرضى العراقيين المصابين بمرض تصلب الاعصاب المنتشر == The Role of Foxp3 and MicroRNAs (20a, 146a, 145, and 155) in a Sample of Iraqi patients with Multiple sclerosis

Author name: زينب عبد الاله عبد الجواد الجوادي
Supervisor name: عائدة رشيد منصور الدرزي | بان عباس عبد المجيد
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Doctorate
Language: English
University location: Baghdad
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Abstract: Multiple sclerosis is a devastating central nervous system autoimmune disorder that is characterized by a series of inflammations, demyelinations, and neurodegenerations that affect the brain and spinal cord. The epigenetic studies specially micro Ribonucleic acid expression represent an important field of researches that probably uncover the obscurities behind the multiple sclerosis pathogenesis. The aims of the present work were to study the expression of micro Ribonucleic acids (20a, 146a, 155, and 145) and forkhead box P3 genes by real time polymerase chain reaction relative quantification technique, to correlate their expression according to different clinical parameters, and to perform chromosomal analysis on the multiple sclerosis patients peripheral blood lymphocytes. A case - control study was performed on 25 newly diagnosed untreated multiple sclerosis patients during their attendance to Multiple Sclerosis clinic, or during their admission to the Neuromedical ward (10th floor) in Baghdad Teaching Hospital, and Special Nursing Home (5th floor) in Baghdad Medical City as well as from the Neuromedical Consultancy Unit in Al - Yarmouk Teaching Hospital in Baghdad city, in addition to 25 apparently healthy controls which were medical staff with no history of having any autoimmune disease or receiving any steroid or immunomodulatory therapy. Furthermore, chromosomal analysis was performed for multiple sclerosis patients. This work was conducted during the period from 1st of April 2015 to 31st of March 2016.From a total of 25 multiple sclerosis patients, 23 of them were females and 2 were males with a female to male ratio (F : M) of 11.5 : 1. The ages of these patients ranged between 18 - 55 years; mean age was 33.32 + 9.45 years. Studying of micro Ribonucleic acids expression revealed a significant down - regulation in micro Ribonucleic acid - 20a and forkhead box P3 gene while up - regulation of micro Ribonucleic acid - 155 expression in multiple sclerosis patients in comparison to controls. Micro Ribonucleic acid - 146a was directly associated with inflammation, and multiple sclerosis disease activity. On the other hand, micro Ribonucleic acid - 145 was not associated with significant changes in its expression in relation to multiple sclerosis disease. Chromosomal analysis was done for multiple sclerosis patients and it was normal.In conclusion, multiple sclerosis is associated with significant changes in micro Ribonucleic acids expression including micro Ribonucleic acid - 20a, micro Ribonucleic acid - 146a, and micro Ribonucleic acid - 155 but not micro Ribonucleic acid - 145 as well as to a significant change in forkhead box P3 gene expression that can be measured by real time polymerase chain reaction technique. Furthermore, multiple sclerosis is not associated withgross chromosomal abnormalities.

الكشف الجزيئي عن فايروس الجي سي وجينات البي ثلاثة وخمسون والشبكية الجيني والبتا - كتنين في المرضى المصابين بالاورام الغدية للقولون والمستقيم == MOLECULAR DETECTION OF JC VIRUS, P53, RETINOBLASTOMA GENES AND ? - CATENIN IN PATIENT WITH COLORECTAL ADENOCARCINOMA

Author name: ابراهيم فاضل ابراهيم الدروبي
Supervisor name: فائزة عبد الله مخلص | بان عباس عبد المجيد
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: تمهيد : يعود فايروس الجي سي الى عائله الفيروسة التوارمية. ينتشر الفايروس بين السكان وعلى نطاق واسع حول العالم. يحمل الفايروس جينة Ag T والتي لها القدره على التكوين السرطاني في الانسجة من خلال التفاعل مع المسارات التنظيمية المختلفه, ويمكن ان تتداخل مع التحكم في دوره الخلية واليات عدم الاستقرار الجيني.العلاقة بين فايروس الجي سي وسرطان القولون وسرطان المستقيم لايزال المنظر ينتضرالقرار النهائي.اهداف الدراسة : 1. تحد يد الدور المحتمل لفايروس الجي سي في سرطان القولون والمستقيم من خلال الكشف والتقدير الكمي لجينة التي وتحميل جينة الاكنوبروتين واظهار الحامض النووي الدي ان اي للفايروس في كل من الخزعات النسيجية السرطانية والطبيعية للقولون والمستقيم. 2. الكشف عن الايجابية المصلية من خلال فحص الاجسام المضادة للفايروس في مصول مجموعه البحث.3. تقدير الرابطة المحتملة بين فايروس الجي سي مع البيتاكاتانين وملاحظة قابلية الفايروس على كبح او تحديد عمل الجينات المشبطة للاورم البي خمسة وثلاثون والشبكية الجيني.طرق العمل : تم تصميم البحث كدراسة مستقبلية للسيطرة على الحالة وانطوت على اخذ الجزعات الطازجة لانسجة القولون والمستقيم من خلال تنظير القولون والمستقيم وقد شملت على 28 مريض مصاب بورم القولون والمستقيم الخبيث وثلاث وثلاثون مريض ممن لم يشخص لديهم ورم سرطاني قي القولون والمستقيم والذين راجعوا وحدة التنظير في مستشفى الاورام ومستشفى بغداد التعليمي ومستشفى اليرموك التعليمي خلال الفتره من حزيران 2013 ولغاية اذار2014. تم الكشف عن جينة فايروس الجي سي ( التي جين) والاكنوبروتين بواسطة جهاز تفاعل سلسلة البلمره وكذلك فحص الحامض النووي الدي ان اي لفايروس الجي سي من خلال التهجين الموقعي وكذلك تم الكشف عن الاجسام المصلية المضادة للفايروس بطريقة الاليزا. تم الكشف عن البيتا - كتنين وبروتين الشبكية الجيني بطريقة المعلمات المناعية والكشف عن البي خمسة وثلاثون بتقنية التهجين الموقعي الفلوري.النتائج• بالنسبة لمجموعة السرطان كان اعلى نسبة تردد للاصابة بورم القولون والمستقيم (35.71%) في المجموعة العمرية مابين 50 - 59 سنة. كان سرطان القولون والمستقيم اكثر شيوعا في الذكور منه في الاناث وبنسبة 1.8 : 1.• اضهر الفحص النسيجي ان النوع الاكثر شيوعا كان بنسبة 85.72% من نوع غدية غير ميوسينية.وان ورم القولون الاكثر شيوعا هو غدية متباينة بشكل معتدل (75%) بين درجات غدية ورم القولون والمستقيم• تم الكشف عن جينة الورم لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل وسجل اعلى تردد للاصابة(57.1%) واعلى نسبة نسخ من الجين في مجموعة السرطان مقارنته بمجموعة السيطرة (27.3%) وكانت نتائج الحمل الفايروسي لمجموعة السرطان بمعدل416.93±217.77 نسخة/مايكروغرام اما مجموعة السيطرة فكانت بمعدل229.866±111.49 نسخ /ميكروغرام والتي اضهرت بدورها فرق كبير بين مجموعات الدراسة.• تم تشخيص جينة الاكنوبروتين لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل في مجموعة السرطان وكانت النتسبة ( 53.6%) وهي اعلى من النسبة التي تم تشخيصها في مجموعة السيطرة والتي كانت (15.2%). الحمل الفايروسي في مجموعة السرطان كان بمعدل 317±129.12 نسخة/ مايكروغرام وهو اعلى من معدل الحمل الفايروسي في مجموعة السيطرة (152.94±10514) نسخة/ مايكروغرام .• وجد اتفاق كبير بين نتائج تشخيص جينة التي وجينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل لمجموعة السرطان ومجموعة السيطرة.• بطريقة التهجين الموقعي لل(دنا) لفيروس الجي سي, اضهرت النتائج ان 53.6% من مجموعة السرطان اضهرو نتائج موجبة لفايروس الجي سي . اما مجموعة السيطرة فاضهرت (30.3%). وكان الفروق ذات دلاله احصائية بين مجموعاتا لدراسة P<0.05 . تبين وجود فروق ذات دلالة احصائية بين مجموعات الدراسة بشان كثافة اشارة ونسبة الاصابة ونمط تورط (P <0.05)، في حين لا توجد فروق كبيرة عثر فيما يتعلق بالمشاركة غدي او اللحمية (P = 0.458)• لم يتم العثورعلاقة ذات دلالة احصائية عند مقارنة ال (دنا ) بواسطة التهجين الموقعي وفقا لدرجات ومواقع الورم.• وجد اتفاق كبير بين نتائج تشخيص جينة التي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التشخيص بطريقة التهجين الموقعي لل ( دنا) بين مجموعات الدراسة.• وجد اتفاق متوسط بين نتائج تشخيص جينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج تشخيص فايروس الجي سي بطريقة التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• فحص المضاد المناعي لفايروس الجي سي بطريقة الاليزا اضهرت وجود مضاد مناعي في 14/ 22(63.6%) مريض من مجموعة السرطان في حين وجد ان 15/28 (53.6%) من مجموعة السيطرة تحمل المضاد المناعي. (p=0.474) وجد توافق ضعيف بين نتائج تشخيص فايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التهجين الموقعي لل (دنا) ونتائج فحص المضاد المناعي في مجموعات الدراسة.• تم الكشف عن توطين البتا كتنين في النووية بطريقة الفحص النسيجي المناعي الكيمياوي وكانت بنسبة12/28 ( 42.86%) من مجموعة السرطان، في حين لم يكشفت على تلطيخ نووي ايجابي في مجموعة السيطرة (P <0.05). لم يتم العثور على علاقة احصائية لا مع درجات الوروم ولا الى موقع الورم.• تم الكشف عن بروتين جينة الشبكية الجيني بتقنية الفحص النسيجي المناعي الكيمياوي.بالنسبة لمجموعة السرطان 10/28 (35.7%) اضهرت تلطيخ نووي ايجابي في حين ان 18/28 (64.3٪) كشفت عن تلطيخ نووي سلبي اما مجموعة السيطره فاضهرت(100%) تلطيخ ننووي ايجابي. لا اتفاق بين نتائج فحص جين الرتينوبلاستوما وجينة التي وجينة الاكنوبروتين وكذلك التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• بطريقة التهجين الموقعي الفلوري تم الكشف عن التعبير الجيني للبي خمسة وثلاثون. فوجد ان خمسة حالات تم شطب موقع البي خمسة وثلاثون من كرومسوم رقم 17. ثلاث حالات وجدت فيها شطب كروموسوم 17 وحالة واحد وجد فيها خليط من شطب موقع البي خمسة وثلاثون وايضا شطب لكروموسوم رقم 17.الاستنتاجاتابرزت هذه الدراسة الدور المحتمل للفايروس الجي سي التي قد تشارك في طرق مختلفة في التسبب في سرطان القولون والمستقيم، والتي تتفق مع دراسات اخرى. على الرغم من هذه الادلة دور JCV في الاورام الخبيثة القولون والمستقيم وoncoproteins في تعزيز التحول لا يزال بعيدا عن الوضوح. | John Cunningham Virus (JCV) a member Polyomaviridae family is a type of human polyomavirus. It’s widespread virus detected in different populations throughout the world. JCV encodes for T - Ag which have oncogenic capability through interaction with different regulatory pathways and can interfere with cell cycle control and genomic instability mechanisms. The association between JCV and colorectal carcinoma still awaits the final conformation. Aims of study : 1. Determine the possible role of JCV in colorectal carcinoma by detection, quantification of T - Ag gene and agnoprotein gene load and demonstrating JCV DNA in both colorectal carcinoma and normal colonic tissue biopsies. 2. Detect the seropositivity of JCV antibodies among the study groups.3. Estimate the possible interaction of T - Ag with B - catenin and P53 and retinoblastoma tumor suppressor genes. Methods : This study was designed as prospective case - control study that involved fresh colonic tissue biopsies taken through colonoscopy from 28 patients with colorectal cancer and from 33 patients who did not have colorectal carcinoma attending to GIT endoscopic unit of Oncology Hospital, Baghdad Teaching Hospital, and Al - Yarmouk Teaching Hospital during the period from June 2013 to march 2014. JCV DNA was detected and quantified by real time PCR for T - Ag gene and agnoprotein genes. Using JCV bioprobe, chromogenic in situ hybridization (CISH) technique was used to detect JCV in tissue biopsies. Serological detection of JCV IgG antibodies was detected by ELISA. B - catenin and retinoblastoma protein products were detected by immunohistochemistry. Tumor suppressor gene P53 was detected by fluorescent in situ hybridization technique. Results : • Among the total 28 colorectal carcinoma cases, the highest frequency 10/28(35.7%) were detected in age group 50 - 59 years and its more frequent in male than female with ratio 1.8 : 1.• Histopathological finding revealed that 24(85.72%) cases were non mucinous adenocarcinoma and moderately differentiated adenocarcinoma was more frequent 21/28 (75%) among grades of CRC.• JCV T - Ag was detected in 16 (57.1%) of CRC group and 9/33(27.3%) of control group, with viral load VL mean 416.93 ± 217.77 copy/µg for CRC group and 229.866 ± 111.49 copy/µg for the control group which in turn showed significant difference between the study groups• Agnoprotein gene was detected in 12/28 (42.9%) of CRC group with VL mean (317 ± 129.12) compared to 5/33 (15.2%) with VL mean (152.94 ± 105.14) in non CRC patient (p=0.016). • Substantial agreement was found between T Ag gene and agnoprotein gene by real time PCR for both CRC group K=0.72 and control group K=0.784.• Using chromogenic in situ hybridization technique JCV DNA was detected in 15/28(53.6%) of CRC compared to 10/33(30.3%) in control group (p=0.065). Significant differences were detected comparing the percentage of JCV DNA in study groups according to intensity, score and signal pattern of involvement (p<0.05).• Substantial agreement was found between T Ag by qPCR and JCV DNA by CISH among the study groups. Moderate agreement was found between JCV agnoprotein gene by qPCR and JCV DNA by CISH among the study groups. • Serological detection of JCV antibodies by ELISA revealed 14/22 (63.6%), 15/28 (53.6%) of CRC group and control group showed positive results respectively. (p=0.474). Poor agreement was found between (T Ag by real time PCR and by CISH) and JCV antibodies by ELISA in the study groups.• Nuclear localization of B - catenin was detected by immunohistochemistry in 12/28(42.86%) of CRC groups, while none of the control group revealed positive nuclear staining (p<0.05). No significant associations found neither to grades nor to the site of tumor.• Retinoblastoma protein was detected by IHC. Only ten out of 28 (35.7%) showed positive nuclear staining, 18/28 (64.3%) of CRC revealed negative nuclear staining. All control group showed positive nuclear staining (p=0.000). No agreement was found between Rb protein and JCV T Ag, agnoprotein gene and JCV DNA by CISH among study groups.• P53 deletion was detected using FISH technique. Five cases out of 25 were with deleted p53 region, 3/25 were with deleted whole chromosome 17, and 1/25 revealed combination signals of deleted p53 region and deleted whole chromosome 17. The results were invalid for statistical analysis.Conclusion : This study highlighted the possible role of JCV which might participate in different ways in the pathogenesis of colorectal carcinoma, which in agreement with other studies. Despite these evidences the role of JCV in colorectal malignancies and its oncoproteins in promoting transformation is still far from clear

تقييم مستوى بعض السايتوكينات الدهنية والبروتين الدهني الواطي الكثافة المؤكسد ومستوى دهون الدم قبل وبعد استعمال الميثوتركسيت في مصل مرضى داء الصدفية : دراسة حالات مرضية - مجموعة ضبط == Evaluation of some serum adipokines, oxidized low density lipoprotein and lipid profile before and after methotrexate treatment of chronic plaque psoriasis (A case - control study)

Author name: حيدر عبد جبار داود العمار
Supervisor name: مناف صالح داود | محمد كاظم الحطاب
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: يعتبر داء الصدفية من الامراض الالتهابية الشائعة التي تصيب الجلد وهو ذاتي المناعة ويصيب الناس بمعدل (2 - 4)% من مجموع سكان العالم. خلايا T المخترقة لانسجة الجلد، الانترلوكينات، والسايتوكينات مثل visfatin)) ، وهرمونhigh molecular weight adiponectin - ذات اوزان جزيئية كبيرة ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL جميعها تؤثر بداء الصدفية. عادة يبدو جلد المريض بداء الصدفية مقشرا ومغلف بلون ابيض فضيا وممكن ان يصيب بقية اجزاء الجسم. يطرا على حياة المريض بداء الصدفية نفس ما يطرا على مرضى السكري من الدرجة الثانية او المرضى النفسيين او مرضى القلب من تغيرات. ويكون المريض بداء الصدفية بدينا مقارنة بعامة الناس. على الرغم من ان معرفتنا بسبب السمنة التي يصاب بها المريض بداء الصدفية الا ان هنالك الكثير من الدراسات التي وضعت بهذا الشان تفترض بان السبب هو افراز السايتوكينات المحفزة للالتهاب بواسطة الانسجة الشحمية ربما هو المسؤل عن تدهور حالة الصدفية. الدراسة الحالية قامت باجراء اختبارات لقياس التغيرات الحاصلة في مستويات انزيم الفيزفاتن ، وهرمون سايتوكين دهني ذات اوزان جزيئية كبيرة HMW - adiponectin، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الوطئ الكثافة المؤكسدox - LDL وكذلك اقتران هكذا تغيرات مع مدى نشاط او استفحال المرض. الاهداف : ان هدف الدراسة هو : لمعرفة التغيرات الحاصلة في مستويات انزيم الفيزفاتنvisfatin ، السايتوكين الدهني(الاديبونكتين) ذات اوزان جزيئية كبيرةHMW - adiponectin ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدoxidized - LDL في مصل مرضى داء الصدفية قبل وبعد العلاج بالميثوتراكسيت بالمقارنة بمستوياتها في مجموعة الضبط وكذلك لتقييم تاثير ذلك العلاج على مستويات الدهون الاخرى في المصل وكذلك بقياس تاثيره على مناطق الصدفية وشدة المؤشرPASI score. اجريت مقارنة بين السايتوكينات الدهنية و(ox - LDL) المؤكسد مع مؤشر كتلة الجسم (BMI) ومنطقة الصدفية وشدة المؤشر (PASI) وقيمهم التخمينية في وباء الصدفية. اخيرا لتاسيس دور المعطيات المذكورة اعلاه في عملية نشؤ المرض( داء الصدفية). المواد وطرق البحث : اشتملت الدراسة على ست وثمانون شخص (86) من الحالات المطابقة في الجنس والعمر المطابقة للدراسة الحالية (43 مصابين بالصدفية و43 اشخاص اصحاء) اما الحالات المصاحبة بارتفاع ضغط الدم او داء السكري قد تم استبعادها من هذه الدراسة. متوسط العمر كان (41±14.37) للمرضى، بينما كان (35±18.66) لمجموعة الضبط. تم اجراء هذه الدراسة في استشارية الجلدية في مستشفى مرجان التعليمي في مدينة الحلة، وتم اجراء الاختبارات في مختبرات المستشفى ومختبر قسم الجهاز الهضمي خلال الفترة بين (حزيران 2013) الى (اب 2014). تم سحب عينات المصل الماخوذ من الدم لاستخدامه من اجل ايجاد مستوى واجراء اختبارات لقياس التغيرات الحاصلة في مستويات الفيزفاتنvisfatin ، السايتوكينات الدهنية ذات اوزان جزيئية كبيرةHMW - adiponectin ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL ومستوى المحتوى الدهني العام في المصل. تم قياس هذه السايتوكيتات في جميع الحالات بواسطة طريقة الفحص المناعي ELISA. تم تقييم نشاط داء الصدفية وكفاءة الميثوتريكسيت بواسطة مؤشر شدة المرض (PASI) وتاثير الميثوتريكسيت على مستويات السايتوكينات الدهنية المذكورة اعلاه والبروتينات الدهنيه المؤكسدة ذات الكثافة الواطئةox - LDL في المصل. النتائج : اظهرت نتائج هذه الدراسة زيادة واضحة (p<0.05) في الفيزفاتنvisfatin ، الاديبونكتن ذات اوزان جزيئيه كبيرةHMW - adiponectin، ونسبة تركيز البروتين الدهني المؤكسد الواطئ الكثافة (ox - LDL) ، ونقصان واضح (p<0.05) في نسبة تركيز البروتين الريتونولي الرباعي الارتباط (RBP - 4) في مصل مرضى الصدفية عندما تقارن مع مجموعة الضبط. اظهرت الدراسة ارتباطا كبيرا بين (ox - LDL) والفيزفاتن visfatin، وبين الاديبونكتين ذو اوزان جزيئيه كبيرهHMW - adiponectin وكذلك البروتين الرباعي الارتباط الريتونوليRBP - 4. فيما اظهرت نتائج الدراسة انه ليس هنالك علاقة بين الفيزفاتن والاديبونكتن ذو اوزان جزيئية كبيرة والبروتين الدهني الواطئ الكثافة المؤكسد ومؤشر كتلة الجسم (BMI) بينما بينت الدراسة علاقة سلبية واضحة بين البروتين الرباعي الارتباطRBP - 4 مع BMI. من ناحية اخرى اكدت النتائج ارتباطا كبيرا مباشرا بين الفيزفاتنvisfatin والاديبونكتن ( HMW) و(ox - LDL) ومؤشر الشدة (PASI) في هذه الدراسة، بينت نتائج الدراسة ايضا ارتباطا سلبيا واضحا بين (PASI) والبروتين الرباعي الارتباط RBP - 4. بالاضافة الى ذلك يوجد اختلاف واضح في معدلات مستوى الدهون في مصل مرضى الصدفية عند المقارنة مع مجموعة الضبط. اخيرا اوضحت الدراسة ، تاثير الميثوتركسيت على مستوى السايتوكينات و(ox - LDL) ومستوى دهون الدم من خلال الانخفاض الكبير في مستوياتها بعد المعالجة وبواسطة متوسط الاختلاف الكبير في قيم (PASI) قبل وبعد المعالجة لمرض الصدفية. الاستنتاجات : تشير نتائج هذه الدراسة الى ان بعض السايتوكينات الدهنية في مصل الدم ومنها انزيم الفيزفاتنvisfatin ، الاديبونكتين ذات اوزان جزيئية كبيرة HMW - adiponectin، والبروتين ذو رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL)) في مرضى داء الصدفية يمكن ان تكون مؤشرات جيدة للتنبؤ بشدة مرض الصدفية وكيفية متابعة العلاج. عند اجراء مقارنة في مرضى داء الصدفية بين السايتوكينات الدهنيه والبروتين الشحمي الواطئ الكثافة المؤكسدox - LDL مع مناطق الاصابة بالصدفية ومؤشر الشدة (PASI) اظهرت الدراسة ارتباط ايجابي ايجابيا مع فيزفاتن، الاديبونكتن ذات اوزان جزيئية كبيرة و(LDL)ox) المؤكسد،، بينما يرتبط سلبيا مع بروتين الرباعي الارتباط الريتنولي RBP - 4بينما اظهرت الدراسة عدم وجود ارتباط بين هذه السايتوكينات ومؤشر كتلة الجسم (BMI).اظهرت الدراسة علاقة قوية بين شدة المرض ومستوى دهون الدم المختلفة في مرضى الصدفية من خلال قياس مؤشر الشدةPASI)) قبل وبعد اخذ علاج MTXكما بينت نتائج الدراسة تاثيرا واضحا للميثوتريكسيت الفموي على مستوى دهون الدم و(ox - LDL) المؤكسد في مرضى الصدفية، بالاضافة الى تحسن سريع في حجم الصدفية، مما يشير الى الدور المناعي لهذا العلاج في داء الصدفية. | Psoriasis is a common inflammatory autoimmune skin disease that affects 2 - 4%of the world population. T - cells infiltration, interleukins and cytokines including adipocytokines like visfatin, high molecular weight - adiponectin(HMW - adiponectin) and retinol binding protein - 4(RBP - 4), together with oxidized low density lipoprotein(ox - LDL) have been implicated in psoriasis pathogenesis. Many epidemiological evidence speculate that patients with psoriasis may be more obese compared with the general population. Although the definite mechanism underlying the correlation between obesity and psoriasis is uncertain, several studies had hypothesized that, the secretion of pro - inflammatory cytokines by adipose tissue may deteriorate psoriasis. Objectives : The aim of the present study is : To investigate the changes of serum visfatin level, HMW - adiponectin, RBP - 4, and oxidized - LDL in psoriatic patients before and after treatment with oral methotrexate therapy in comparison to their levels in control subjects to evaluate the impact of this drug on their serum levels and on psoriatic lesions by measuring psoriasis area and severity index(PASI). To ascertain the association between these adipocytokines and oxidized - LDL with body mass index(BMI) and psoriasis area and severity index(PASI) and their predictive value in plaque psoriasis . Finally, to establish the role of above mentioned parameters in the pathogenesis of psoriasis. Subjects and Methods : A total of 86 subjects (43 with Psoriasis and 43 healthy controls) were enrolled in this study. Their mean age was (41± 14.37) for patients, and (35.81± 18.66) for the control group. The study conducted in dermatology outpatient clinic in Merjan Teaching Hospital in Hilla City, and investigations were done in laboratory units of the hospital through the period from June 2013 to September 2014. The sera obtained from blood were used to determine the level of serum visfatin, HMW - adiponectin, RBP - 4, oxidized - LDL by enzyme - linked immunosorbent assay(ELISA), while lipid profile levels were estimated by spectrophotometric kits. Assessment of psoriasis disease activity and methotrexate efficacy were done by psoriasis area and severity index (PASI)score and methotrexate effects on serum levels of the above mentioned adipokines and oxidized low density lipoprotein.Exclusion criteria include : any person with hypertension, diabetes mellitus, pregnancy, smoking, alcoholism, and other medical diseases even other types of psoriasis disease.Results : Results of this study showed significant increase (p˂0.05) of serum visfatin, HMW - adiponectin, and oxidized - LDL concentrations, and significant decreases (p˂0.05) of retinol binding protein - 4(RBP - 4) concentration in sera of plaque psoriasis when compared with control group. There was a significant correlation between oxidized - LDL and visfatin, HMW - adiponectin and retinol binding protein - 4(r = 0.81, 0.68, ̵ 0.77)(p value˂0.001) respectively . Regarding the anthropometric parameters, this study shows no correlation between visfatin, HMW - adiponectin, RBP - 4, oxidized - LDL and body mass index(BMI)(r= 0.121, 0.043, - 0.170, - 0.049)respectively(p˃0.05). The findings of the present study confirm a significant association between visfatin, HMW - adiponectin, RBP - 4 and oxidized - LDL and PASI score(r= 0.77, 0.64, ̵ 0.77, 0.72)(p˂0.001) respectively. Additionally, there was a significant mean difference in lipid profile in serum of psoriatic patients when compared to control subjects(p value˂0.001). Finally, the impact of systemic methotrexate on above adipocytokines and oxidized - LDL was determined by significant reduction of their levels after treatment and by a significant mean difference in PASI score before and after treatment in psoriatic lesions size. Conclusions : Results obtained by this study indicate that some adipocytokines including serum visfatin, HMW - adiponectin and RBP - 4 levels, in addition to oxidized - LDL measured in patients with plaque psoriasis were closely associated with disease severity and could be used for prediction and treatment follow up.In patients with plaque psoriasis the PASI score is positively associated with serum visfatin, HMW - adiponectin and oxidized - LDL, while negatively correlated with serum retinol binding protein - 4 level, while the study results revealed no significant correlation between BMI and study parameters. This results may indicate that, these parameters could be independently regulated through the path of psoriasis process.The study findings confirm the association between psoriasis and abnormal lipid profile and also the study showed a significant correlation between lipid profile and PASI score(p˂0.05).The study results showed the good effect of oral methotrexate upon the serum adipocytokines and oxidized - LDL - c levels in psoriatic patients, in addition to better remission of psoriatic lesions, which indicate the immune modulatory role of this drug in psoriasis

دراسة جزيئية وبكتريولوجية لبكتريا Bacteroides fragilis المعزولة من النساء المصابات بالتهاب المهبل البكتيري == Molecular and Bacteriological Study of Bacteroides fragilis Isolated from Women with Bacterial Vaginosis

Author name: زهراء قيس سلمان
Supervisor name: الهام عباس بنيان الساعدي | بشرى جابر عمران
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: شملت هذه الدراسة 150 مريضة يعانين من التهاب المهبل البكتيري ، تم جمع ما مجموعه 300 مسحة من عنق الرحم وافرازات مهبلية من هؤلاء المريضات بمعدل مسحتين اثنتين لكل مريضة و150 عينة دم جديدة تم جمعها من المريضات عند مراجعتهن الى مستشفيين في محافظة بابل هما : مستشفى بابل للنسائية والاطفال التعليمي ، ومستشفى الحلة التعليمي العام، و150 عينة دم جديدة كمجموعة سيطرة ، خلال الفترة من شباط الى تشرين الاول 2016. باستخام معايير امسلز تم تشخيص النساء اللواتي يعانين من التهاب المهبل البكتيري. وقد خضعت المسحات لطرق مختلفة لتحديد B.fragilis باستخدام الاساليب البكتريولوجية التقليدية. وجد ان 31 عزلة موجبة (20.6٪) باستخدام الاوساط الانتقائية. فضلا عن ذلك، تم تطبيق طرق الكشف الجزيئي باستخدام المعلم الوراثي المستند على دور جين 16 sRNA ، وكانت 44 عزلة (29.33٪) ايجابية وتعود لبكتريا B.fragilis وكذلك استخدم المعلم الوراثي nanH حيث كانت 39 عزلة (88.6٪) ايجابية. بالاضافة الى ذلك، تم فحص العزلات المعزولة على الاوساط الانتقائية الخاصة بالبكتريا للكشف عن قابلية البكتريا للالتصاق (adherence ability) بالخلايا المخاطية. كما تم التحري المظهري عن عملية تكوين الاغشية الحيوية (Biofilm formation) للعزلات المدروسة باستخدام فحص tissue culture plate (TCP) test اذ كانت مكونة للاغشية بنسبة (100%). حيث اظهرت العزلات 31 الموجبة المستخرجة من الوسط الانتقائي قدرة البكترياعلى تكوين ال Biofilm وكانت نسبة تكوين البيوفيلم (83.9٪) و(16.1٪) للوضع العالي والمعتدل على التوالي، في حين لم تكن هناك عزلات غير قادرة على تشكيل بيوفيلم. اما فيما يتعلق بتاثير بوفيدون - اليود بنسبة 10٪ على تكوين البيوفيلم، فقد اظهرت نتائج هذه الدراسة ان قدرة البكتريا على تكوينه قد تقلصت لتكون نسبة التشكيل قوي (6.5٪ (ومعتدل (71٪) و(22.6٪) لم يظهر اي تشكيل للبيوفيلم . تم فحص تاثير 10% povidone - iodine المستخدمة في الطب النسائي على انتاج الاغشية الحيوية . وقيمت الدراسة قابلية B.fragilis على انتاج bacteriocin وتاثيرها على بعض العزلات البكتيرية. اظهرت النتائج ان لعزلات البكتريا قدرة تازرية لتثبيط نمو العزلات المدروسة بنسب. فضلا عن ذلك، هدفت هذه الدراسة الى التعرف على علاقة التغايرالجيني(TLR - 4 Polymorphism) باستخدام تقنية البلمرة والقطع بالانزيم (PCR - RFLP) .وتبين من النتائج وجود ثلاث انماط جينية وكانت نسب انتشارها في مجموعة المرضى CC(36.0%) وThr399Ile : CT(12.0%) ,TT(52%) بينما نسب انتشارها في مجموعة السيطرة كانت TT(34.0%) , CC(60.0%) and CT(6.0%) وقد اظهرت وجود اختلاف وهو اختلافا معنويا, فيما بلغت نسبة انتشار الاليل T في المرضى (58%) بالمقارنة للسيطرة (37%) مع وجود اختلاف معنوي .وفي المقابل سجل وجود ثلاث انماط جينية في موقع طفرة Asp299Gly : AA(46.0%) ,GG(50.0%) and GA(4.0%) في مجموعة المريضات بينما في مجموعة السيطرة كانت نسب الانتشار AA(34.7%) , GG(59.3%) and GA(6.0%) مع عدم وجود فارق معنوي بينهما , اما نسبة انتشار الاليل G (52%) في مجموعة المريضات بالمقارنة بمجموعة السيطرة (62%) ويعد فارقا معنويا بينهما . تناولت الدراسة تحليل تسلسل جينات معينة من عزلات B.fragilis وكشفت نتائج التحليل للاحماض الامينية لجين Enterotoxin حيث تم تحديدها في 117/117 (100٪ (بمقارنة تسلسلها مع تلك التي في قاعدة البيانات في بنك الجينات وكانت نسبة الثغرات بينهما (0)٪ ، اما بالنسبة للعزلة الثانية فقد تم التعرف على نتائج التحليل للاحماض الامينية لجين Enterotoxin في 116/116 (100٪) وبثغرات 0/116 (0٪)، اما بالنسبة لجين Phospholipaseتم تحديدها في 129/129 (100٪) مع ثغرات 0/1129 (0٪) لكل من العزلتين . اخيرا تم تحليل العلاقات التطورية بين العزلات محلية من B.fragilis والعزلات العالمية من B.fragilis باستخدام تسلسل الجينات لل Enterotoxin وPhospholipaseحيث اظهر تحليل تسلسل الحمض النووي لجين Enterotoxin ان عزلات B.fragilis المحلية (NO.1) و(No.2) كانت مرتبطة ارتباطا وثيقا بعزلة B. fragilis المسجلة عالميا (AB026625.1) و(U67735.1) ونسبة التغير الجيني الكلي في شجرة التطور (0.01%) في حين ان نتائج تحليل النشوء والتطور من تسلسل جينات Phospholipase كشفت ان نفس العزلات المحلية (NO.1) و(No.2) كانت مرتبطة ارتباطا وثيقا بالعزلة المسجلة عالميا (CP011073.1) ، ونسبة التغير الجيني الكلي في شجرة التطور كانت (0.003 - 0.005%). | This study included 150 women with bacterial vaginosis (BV), a total of 300 swabs (as two high vaginal swabs for each woman and vaginal discharge ) were taken from each woman. In addition to fresh blood samples were collected from the same 150 women with bacterial vaginosis and 150 healthy women as control group , these women admitted to the out - patient clinics of Gynecology and Obstetrics , in two hospitals of Babylon Province : Babylon Maternity and Pediatrics Teaching Hospital, and Al - Hillah General Teaching Hospital during the period from February until October 2016. Women suffering from bacterial vaginosis were diagnosed according to Amsels criteria .The high vaginal swabs were subjected to different methods for identification of Bacteroides fragilis by using traditional bacteriological methods . It was found that 31(20.6%) isolates were recovered by using selective media . Furthermore , molecular detection methods was applied by using 16s rRNA gene ,where 44(29.33%) isolates were positive , and using nanH gene where 39(88.6%) isolates were positive out of the 44 isolates , these genetic markers were used for confirmation detection of B.fradilis isolates . In addition , the 31 isolates recovered from selective media were investigated to detect adherence of B.fragilis to mucosal epithelia cells .Also , biofilm formation was tested in semi quantitative microtiter plate test . The result revealed that all isolates were biofilm former , biofilm formation were accounted (83.9%) and (16.1%) for high and moderate mode respectively , while there were no isolates that do not express biofilm formation . Regarding on effect of 10% povidone - iodine on biofilm formation ,the result of this study demonstrated that internal douche effectively reduced the viability of biofilm forming to strong (6.5%) , moderate (71%) and (22.6%) shown no biofilm formation . The study was also evaluated the susceptibility of bacteriocin produced by B.fragilis on some isolates of bacteria . The result revealed that B.fragilis have synergistic ability to inhibition the growth of studied isolates with different ratios . Additionally, this study aimed to investigate the relation of Asp299Gly and Thr399Ile Single Nucleotide Polymorphism (SNPs) in the Toll - like receptor - 4 (TLR - 4) with the incidence of bacterial vaginosis .The result elucidated that at the site of Thr399Ile SNP, there were three genotypes for this SNP among patients ; TT,CC and TC with frequency of (52%) ,(36.0%) and (12.0%) respectively , whereas , the genotype frequency among control group were TT(34%) ,CC(60%) and TC(6.0%) with significance differences between patients and control . However, the frequency of allele T (mutant) was higher among patients (58%) than control (37%) with significant difference. The site of Asp299Gly SNP also showed three genotype among patients with frequency of AA(46.0%), GG(50.0%) and GA(4.0%) , whereas the genotype frequency among control group were AA(34.7%) , GG(59.3%) and GA(6.0%) with no significant differences between patients and control . Additionally , the frequency of allele G(mutant) was higher among control(62%) than patients (52%) with significant differences between them . The study deal with sequence analysis of specific genes of B.fragilis isolates revealed that the analysis results of amino acids for enterotoxin gene was identified in 117/117(100%) by comparing its sequence with that in the database in gene bank by Blast program with gaps 0/117(0%) and for the second isolate the analysis results of amino acids for enterotoxin gene was identified in 116/116(100%) and with gaps 0/116(0%) , while for phospholipase gene was identified in 129/129(100%) with gaps 0/1129(0%) for first isolates.Finally ,the phylogenetic relationships between two local isolates of Bacteroides fragilis and Bacteroides fragilis global isolates were analyzed using the enterotoxin and phospholipase genes sequence. The DNA sequencing analysis of enterotoxin gene revealed that B. fragilis local number (No.1and No.2) isolates were closely related to isolation B. fragilis recorded globally NCBI - BLAST B. fragilis (AB026625.1) and (U67735.1) , whereas other NCBI - Blast B.fragilis enterotoxin gene were show different out of tree at total genetic change (0.01%) , while the phylogenetic analysis results of phospholipase gene sequences revealed that B. fragilis local isolates ( No.1and No.2) were closely related to isolation B. fragilis recorded globally NCBI - BLAST B. fragilis (CP011073.1) , whereas other NCBI - Blast B.fragilis phospholipase gene showed difference out of tree at total genetic change (0.003 - 0.005 %).

الكشف الجزيئي للفايروس البشري المتوجه للخلايا اللمفاوية التائيه - النوع الاول والتعبير البروتيني ل P27, CDK6,CDK4 وBcl - 2 في سرطان الغدد اللمفاوية الهودجكن واللاهودجكن == Molecular Detection of Human T Lymphotropic Virus Type - 1 (HTLV - 1) and Expressional Proteins of CDK4, CDK6, P27 and Bcl - 2 in Hodgkin?s and Non - Hodgkin?s Lymphomas

Author name: نور سامي عبود الليباوي
Supervisor name: جواد كاظم طراد الخفاجي | شاكر حماد محمد العلواني
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: صممت هذه الدراسة على انها دراسة بحثية ذات اثر رجعي ,وسيطره . اذ اشتملت على120خزعة نسجية من منطقة الغدد اللمفاوية المحفوظة بالفورمالين والمطمورة بشمع البارافين.جمعت هذه النماذج من ارشيفات الانسجة المرضية لمختبرات مستشفى الحلة التعليمي/ محافظة بابل , مستشفى الحسين التعليمي/ محافظة كربلاء ,مستشفى الصدر التعليمي/ محافظة النجف ,مستشفى الحسين التعليمي/ محافظة المثنى ومستشفى القادسية التعليمي/ محافظة القادسية وكذلك العديد من مختبرات الانسجة المرضية الخاصة الموجودة في بابل ,بغداد, كربلاء, القادسيه, المثنى اذ جمعت هذه العينات للفترة من اذار 2016 الى كانون الاول 2016. هذه النماذج تعود للمرضى المصابين بسرطان العقد اللمفاوية للسنوات من 2011 لغاية 2016 .قسمت هذه العينات حسب المجموعات التالية : - 1 - اربعون قطعة نسيجية لمفاوية مصابة بسرطان العقد اللمفاوية نوع هودجكن .2 - اربعون قطعة نسيجية لمفاوية مصابة بسرطان العقد اللمفاوية نوع اللاهودجكن .3 - اربعون قطعة نسجية لعقد لمفاوية (لايظهر عليها اي تغيرات مرضية نسيجية) ظاهريا سليمة استعملت كمجموعة سيطرة .بعد التقطيع النسيجي لخزع العقد اللمفاوبة وتصبيغها باستخدام صبغتي الايوسين والهيماتوكسلين تم اجراء الفحص التاكيدي الدقيق لغرض التشخيص النهائي من قبل اخصائي الامراض النسيجية لهذه الدراسة. تم تصميم الجانب العملي لهذه الدراسة الذي اتخذ مسارين رئيسين : - 1. التحديد الجزيئي للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بفحصها داخل خلايا الانسجة اللمفاوية حيث تم انجازه بوساطة استخدام تقنية التهجين الموضعي ذات الحساسية العالية .2. اجراء دراسة كيميائية - نسيجية مناعية لوصف الحالة التعبيرية ل CDK4 وCDK6 ومثبط CDK (p27) وفحص الجينات السرطانية للخلايا اللمفاوية B (Bcl - 2 ) في الانسجة المصابة بالهودجكن واللاهودجكن والعقد اللمفاوية السليمة ظاهريا (غير مصابة).ان مجمل النتائج التي تم الحصول عليها هذه الدراسة كانت كما يلي : 1 - نسبة الذكور( (57.5% المصابين بسرطان الغدد اللمفاوية الهودجكن واللاهودجكن ومجموعة السيطرة كانت اعلى من نسبة الاناث (42.5%) في هذه المجاميع الثلاثة. حيث كانت نسبة الذكور/اناث هي1 : 1.35.2 - اظهر الفحص النسيجي ان النسبة العليا من مرضى الهودجكن كان من الدرجة المنخفضة (42.5%) يتبعها الدرجة المتوسطة (35%) وادنى نسبة هي الدرجة العليا (22.5%). بينما النسبة العليا من مرضى اللاهودجكن كانت من الدرجة المنخفضة والمتوسطة %37.5)) وادنى نسبة هي الدرجة العليا ( 25%).ا - نتائج الفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول وCDK4 وCDK6 ومثبط CDK (p27 ) وكذلك الجينات السرطانية للخلايا المفاوية B (Bcl - 2 ) في الانسجة المصابة بالهودجكن : 1 - النتائج الموجبة لجين HBZ للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بتفاعل التهجين الموضعي كانت ( 22.5%) 2 - اظهرالاختبارالكيميائي النسجي المناعي لـ p27 ان 35% من الانسجة كانت موجبة .3 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK4 ان 35% من الانسجة كانت موجبة .4 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK6 ان 57.5 % من الانسجة كانت موجبة . 5 - اظهرالاختبارالكيميائي النسجي المناعي لـ Bcl - 2ان47.5 % من الانسجة كانت موجبة .6 - تم تمييز علاقة طردية قوية تربط بين تحديد والانتشارالتعبيرالبروتيني والكثافة للفيروس وCDK4 .7 - وجدت علاقة طردية قوية بين تحديد والانتشارالتعبيرالبروتيني والكثافة للCDK4 وCDK6 .وايضا وجدت علاقة بين تحديد وكثافة ال CDK6 وBcl - 2.8 - تم تمييز علاقة طردية قوية تربط بين تحديد والانتشارالتعبيرالبروتيني والكثافة ل p27 وعمر المرضى. كذلك علاقة طردية بين p27 وBcl - 2.ب‌ - نتائج للفايروس البشري المتوجه للخلايا اللمفاوية التائية من النوع الاول وCDK4 وCDK6 ومثبط CDK (p27 ) وكذلك الجينات السرطانية للخلايا المفاوية B (Bcl - 2 ) في الانسجة المصابة باللاهودجكن : 1 - النتائج الموجبة لجين HBZ للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بتفاعل التهجين الموضعي كانت (45%) بينما لم تظهر اي حالة موجبة بتفاعل التهجين الموضعي للفايروس ضمن مجموعة السيطرة .2 - اظهرالاختبارالكيميائي النسجي المناعي لـ p27 ان 42.5% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة للp27 بواسطة الاختبارالكيميائي النسجي المناعي ضمن مجموعة السيطرة.3 - اظهرالاختبارالكيميائي النسجي المناعي لـCDK4 ان 50% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السيطرة.4 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK6 ان 72.5% من الانسجة كانت موجبة . بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السيطرة.5 - اظهرالاختبارالكيميائي النسجي المناعي ل Bcl - 2 ان 72.5% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السطرة.6 - تم تمييز علاقة طردية قوية تربط بين تحديد الفيروس وBcl - 2 وكذلك وجدت علاقة قوية طردية تربط بين الانتشارالتعبيرالبروتيني والكثافة للفايروس وCDK6.7 - وجدت علاقة طردية قوية بين تحديد والانتشارالتعبيرالبروتيني والكثافة للBcl - 2 وCDK6 .وايضا وجدت علاقة بين تحديد وكثافة ال p27 وBcl - 2 فقط .كذلك وجدت علاقة عكسية بين عمر المريض وBcl - 2 .8 - تم تمييز علاقة طردية تربط بين كثافة CDK4 وp27 وكذلك بين CDK4 وBcl - 2

دراسة بكتيرية ووراثية لبكتريا Gardnerella vaginalis وعلاقتها بالولادة المبكرة == Bacteriological and Molecular study of Gardnerella vaginalis association with preterm labor

Author name: ازل علاء عباس الربيعي
Supervisor name: الهام عباس بنيان الساعدي | لميس عبد الرزاق عبد اللطيف
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: In this study , one hundred fifty samples were collected from paitents with preterm labor (PTL), who have been attending to Babylon Maternity and pediatric hospital and Al - Hilla Teaching Hospital, at the period from February to October 2016. Two swabs were collected one for culturing and the other for direct extraction for isolation Gardnerella vaginalis.Out of the 150 samples only 6(4%) on culture and 30 (20%) on molecular level isolated from preterm labor caused by Bacterial vaginosis , Urinary tract infection and aborted women. The results isolation and laboratory diagnosis as well as biochemical test approved that there is only 6 isolates belong to G.vaginalis confirmed by using Vitek 2 system and molecular detection by specific primers.Preterm birth is one of the most common causes of neonatal morbidity and mortality. Associated with sub sequent preterm labor in up to 40% of cases as shown in our resultsThe isolates are varied in their ability to produce sidrophore and extracellular protease in addition to their hemolytic activity .Adherence activity were investigated and indicate that G.vaginals have pili on her surface help her to attached and initiated infection Metronidazole was effective in prevent adherence at low concentration (5%) was 100% percentage.All isolates are able to produce biofilm in different level which provided as main step in pathogenesis caused it can evade and evoke the action of immune response and antibiotics , Alum , sodium chloride and sodium bicarbonate were shown very high effectively to eradicationSummaryIIG.vaginalis biofilm which gave results (0%) whereas the thymol extract (carvacrol) were moderate in action gave (50%) effect on Biofilm .Results also preveld that increased of pH value give a chance to overgrowth of G.vaginalis .The results indicated that Lactic acid considered as a limiting factors preventing growth of G.vaginalis when its grown with lactic acid , measured by optical Density (OD) the high production level is (1.901) in 2% concentration Whereas the lowest level is (0.32) in 10% concentration.Isolates Have ability to produce wide spectrum Bacteriocin which inhibited growth of both Gram positive and Gram negative bacteria that founded in vagina environment, Staphylococcus saprophyticus were the most bacteria that inhibited .extracted Vaginolysin was have a completed hemolytic activity in different temperatures (37 - 38 - 40 - 42). The results showed that its activity as were increased in high temperature.Recent study were clarified that purified vaginolysin was active in presence of cholesterol in media, gave (1.205) growth rate measured by the optical density at (100%) concentration. vaginolysin is cholesterol - dependent cytolysin (CDCs) toxin.Antimicrobial activity were investigated to the extracted Vaginolysin to evaluation of its activity depending on inhibition zone, the results showed that candida is more affective between the other microorganism gave (1.3)mm.Some virulence factor have been detected in molecular level ( Vly - SI - LuxS - LexA - rec a - Pl - Fur - Omp - Gal - protease ). Which wereSummaryIIIvaried in production may be as a results to the un presence of this gene in bacteria , may be silent gene or due to the amount of bacteria in sample are rare.Three Antibiotics were used at a molecular level (Tet b) were varied between resistance and sensitive to this gene (10) sample are resist,(RdxA) all sample are sensitive to its gene at percentage (100%), whereas (erna)gene were resist in all samples (100%).The results show there was no sequence homology between Mycoplasma spp. and G. vaginalis in the adhesion level this were detected by P30/P32 specific primer .The outcome of RFLPS - PCR is confirmed that G.vaginalis is virulent and have a role in preterm labor by degradation of membranes with the association of variable Silidase and presence of β - Galactosidase .MBL (mannose binding lectin ) levels were have a direct role pregnancy. It is also apparent that MBL plays a role in the defense against vaginal infection. MBL is essential to successful pregnancy in regards to maternal defense against infection, recent study indicate that MBL deficiency related to pregnancy outcome disorderedthis considered the first study of mannose in Iraq .DNA sequencing were done for two main virulence factor Phospholipase gene and Quorum sensing gene which showed some variation , then recorded in NCBI - gene sequencing as first descriped in Iraq.

التاثير المايكروبي المضاد لدقائق الفضة النانوية ضد بكتريا الزوائف الزنجارية المعزولة من عينات سريرية == The Antimicrobial Effect of Silver Nano Particles (AgNPs) Against Pseudomonas aeruginosa Isolated from Clinical Samples

Author name: سارة عقيل حسن التميمي
Supervisor name: هدى هادي الحسناوي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: اجريت هذه الدراسة للكشف عن التاثيرالمايكروبي المضاد لدقائق الفضة النانوية ضد بكتريا الزوائف الزنجارية المعزولة من عينات سريرية, تم جمع 100 عينة من مصادر سريرية مختلفة, تضمنت العينات السريرية عينات الحروق , والادرار, والدم, واصابات الاذن, والقشع تم اخذ هذه العينات من المرضى الراقدين في مستشفى الحلة التعليمي خلال الفترة من تشرين الثاني 2016 الى كانون الثاني 2017. زرعت جميع العينات على وسط السترمايد, وسط اكار الدم, وحضنت كل الاطباق هوائيا بدرجة حرارة C°37 لمدة 18ساعة. تم تشخيص بكتريا الزوائف الزنجارية بالاعتماد على الصفات المظهرية والكيموحيوية.كان توزيع العزلات كالاتي : 22(52.4%) من عينات الحروق, 8(19%) من عينات الادرار, 14.3)6%) عينة من المرضى المصابين بالالتهاب الاذن الخارجية, 4(9.5%) من عينات الدم,2(4.7%) من عينات القشع. اظهرت نتائج اختبار الحساسية باستخدام طريقة الانتشار في الوسط ان جميع العزلات(26) كانت مقاومة لكل من الببراسلين (100%), السيفوتاكسيم (100%),بينما نسبة المقاومة لكل من الاميكاسين (88%), سيفترياكسون (65%), جنتامايسن (62%), بينما وجدت نسبة مقاومة قليلة للسفتازديم (15%), وكل العزلات كانت حساسة (100) لكل من الميروبينيم, اوفلوكساسين, نورفلوكساسين والسبروفلوكساسين. تم في هذه الدراسة ايضا الكشف عن تكوين الاغشية الحيوية بواسطة طريقتين : طريقة الطبق الزرعي النسيجي (TCP) وطريقة صبغة الكونكو الحمراء (CRA) فكانت النتائج كالاتي : وجد ان في طريقة الطبق الزرعي النسيجي كانت جميع عزلات الزوائف الزنجارية والبالغ عددها (9) منتجة للاغشية الحيوية, (4) من اصل (9) (44%) من عزلات الزوائف الزنجارية انتجت مستعمرات سوداء بواسطة طريقة صبغة الكونكو الحمراء. وكذالك في هذه الدراسة تم الكشف عن تاثير جزيئات الفضة النانوية ((AgNPs على تكوين الاغشية الحيوية باستخدام تراكيز مختلفة (µg/ml 2.5, µg/ml 5, 10 µg/ml, 20µg/ml),حيث اظهرت النتائج ان اعلى فعالية لجزيئات الفضة النانوية كانت عند التركيز (20) وبنسبة تثبيط (100%), من جهة اخرى تم اختبار التاثير البكتيري المضاد لجزيئات الفضة النانوية ضد مختلف انواع العزلات البكتيرية باستخدام طريق التخافيف في الوسط الصلب وتراكيز مختلفة من جزيئات الفضة النانوية (12.5 µg/ml, 25 µg/ml, 50 µg/ml, (100 µg/ml, 200 µg/ml وكانت العزلات كالتالي : ـ(S. aureus, E. coli, E. cloacae, K. pneumonia, P. aeuroginosa)اظهرت النتائج ان اعلى فعالية لجزيئات الفضة النانوية كانت عند التراكيز(100 µg/ml, 200 µg/ml).ايضا تم تعريض ظاهرة السوارمنك في عزلات الزوائف الزنجارية لتراكيز مختلفة من جزيئات الفضة النانوية (2.5 µg/ml, 5 µg/ml, 10 µg/ml ), ووجد ان اعلى نشاط لجزيئات الفضة النانوية ضد ضاهرة السوارمنك وانتاج البايوسيانين كانت بتركيز ((10 µg/ml, 5 µg/ml .تم زراعة جميع عزلات بكتريا الزوائف الزنجارية (26)عزلة بوجود نترات الفضة (AgNO3) بتراكيز تتراوح (100 µg/ml - 400 µg/ml ) باستخدام طريقة التخافيف في الوسط الصلب مع قدرات مختلفة لانتاج البيوسيانين. اظهرت النتائج ان جميع العزلات 26((100 المنتجة للبايوسيانين كانت مقاومة للسلفر نايتريت (AgNO3) من خلال ظهور نمو في الطبق الحاوي على التراكيز المذكورة نترات الفضة (AgNO3).في هذه الدراسة تم اختبار بكتريا الزوائف الزنجارية لانتاج البكتريوسين باستخدام عزلات بكترية مختلفة تضمنت العزلات (امعائية مذرقية,مكورات العنقودية الذهبية,اشريكية قولونية, كلبسيلة رئوية). كذلك تم العثور على مجموعة واسعة من منطقة تثبيط (24mm - 30mm) في الانواع البكتيرية اعلاه مع قطر (30mm, 28mm, 24mm, 30mm) على التوالي. عزلات (امعائية مذرقية, كلبسيلة رئوية) كانت الاكثر تاثر والتي انتجت قطر تثبيط ((30mm. في هذه الدراسة ايضا تم تقييم علاقة مقاومة الفضة مع انتاج بيوسيانين في بكتريا الزوائف الزنجارية متعددة المقاومة للمضادات الحيوية. في هذه الدراسة تم استخدام تقنية تفاعل البلمرة المتسلسل للتاكد من وجود بعض الجينات. في هذه التقنية، تم فحص جينات bla - IMP)) التي تشفر الى انزيم ميتالو - β - لاكتاميز المسؤول عن مقاومة ايميبينيم وجينات (silS ,silE) التي تشفر لمقاومة الفضة في عزلات بكتريا الزوائف الزنجارية بواسطة استخدام برايمرت خاصة. وقد وجد ان جميع العزلات ال 26 (100%) من بكتريا الزوائف الزنجارية كانت سلبية لهذه الجينات. كما تم استخدام اثنين من البرايمرات المحددة للكشف عن فينازين (بيوسيانين) (phzS, phzM). اظهرت النتائج لل (phzM) ان من اصل 26)) عزلة , 21 (80.8%) اعطت نتيجة ايجابية, , فيما يخص (phzS gene) وجد ان 14(% 53.9) عزلة كانت حامله لهذا الجين . والخاتمة ان جزيئات الفضة النانوية (AgNps) تمتلك تاثير ضد الاغشية الحيوية وايضا لها تاثير بكتيري مضاد ضد بكتريا الزوائف الزنجارية وانواع بكتيرية اخرى, والتي من الممكن استخدامها كعلاج بديل. فيما يخص انتاج البايوسيانين ومقاومة الفضة، ظاهريا كان هناك تاثير البايوسيانين على مقاومة الفضة ولم تكن مقاومة الفضة بسبب نقل وجود جينات (silS, silE). | This study was conducted to detect the antimicrobial effect of silver nanoparticles (AgNPs) against Pseudomonas aeruginosa isolated from clinical samples, total of 100 specimens were collected from different clinical sites included urine, burns, ear, blood , and sputum. The specimens were taken from patients admitted to Al - Hilla Teaching Hospital during a period extended from November 2016 to January 2017. All specimens were cultured on selective medium (cetrimide agar), blood agar, then incubated at 37°C for 18 hr. The isolated of P. aeurginosa were diagnosed depending on morphological and biochemical characteristics.The distribution of P. aeurginosa isolates was as follows : 22(52.4%) isolates from burns, 8(19%) isolates from urine, 6(14.3%) isolates from patients with otitis externa, 4(9.5%) isolates were isolated from blood and 2(4.7%) isolates from sputum. Antibiotic susceptibility test using disc diffusion test revealed that all isolates (26) were resistant (100%) to Piperacillin, Cefotaxime. While the resistance rate for Amikacin (88%), Ceftriaxone (65%), Gentamicin (62%), but low resistance rate was found to Ceftazidime (15%) and all isolates were sensitive (100%) to Meropenem, Ofloxacin, Norfloxacin and Ciprofloxacin. The biofilm formation in P. aeurginosa isolates was investigated by two methods include, tissue culture plates method (TCP) and congo red agar method (CRA). The results showed that all isolates 9 (100%) were found to be biofilm producer when investigated by TCP assay, and 4 out of 9 (44. %) isolates of P. aeurginosa were producing black colonies when detected by CRA method.The effect of AgNps on biofilm formation was tested by using different concentrations of silver nanoparticles (2.5 μg/ml, 5 μg/ml,10 μg/ml, 20μg/ml). The results showed that the highest activity was at a concentration of 20 μg/ml, with an inhibition rate of 100%. On the other hand the antibacterial activity of AgNps was tested against different bacterial species isolates (Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomnas aeurginosa) using agar dilution method with different concentrations of silver nanoparticles (12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml). The results showed that the highest activity was at a concentration of (100 μg/ml and 200μg/ml). On the other hand Swarming motility in P. aeruginosa isolates was detected and subjected to different concentrations of (AgNps) (2.5 µg/ml, 5 µg/ml, 10 µg/ml), it was found that the highest activity of AgNps against swarming motility and pyocyanin production of P. aeurginosa was at a concentration of (5 µg/ml, and 10μg/ml).The relation of silver resistance with pyocyanin production in P. aeruginosa was assessed. Screening test for silver resistance was performed by agar dilution method, all (26 isolates) of P. aeruginosa were cultivated in the presence of AgNo3 at a concentration (100 µg/ml - 200 µg/ml, 400µg/ml) using agar dilution method with differing capacities for pyocyanin production. All isolates of P. aeurginosa 26(100%) that produce pyocyanin were found to be resistant to AgNo3.In this study P. aeruginosa isolates were tested for bacteriocin activity by the agar well diffusion method using different bacterial isolates : E. coli, K. pneumoniae, S. aureus, E. cloacae. Wide range of inhibition zone (24mm - 30mm) was found against above bacterial species with diameter (28mm, 30mm, 24mm, 30mm), respectively. The most affected bacteria were K. pneumoniae and E. cloacae isolates, which produce inhibition zone (30)mm.PCR technique was used in this study for investigation some genes. In this technique, blaIMP gene that encode Metallo - β - lactamase enzyme responsible for imipenem resistance and (silE and silS) genes that encoding silver resistance were investigated in P. aeuroginosa isolates by using specific primers. It was found that all 26 (100%) isolates of P. aeuroginosa were negative for these genes. Also two specific primers were used to detect phenazine (pyocyanin) modifying genes (phzM, phzS). According to phzM gene, it was found that out of 26 P. aeuroginosa isolates, 21 (80.8%) isolates gave positive results at 330 bp, in regard to phzS gene, it was found out of 26 P. aeurginosa isolates, 14 (53.9%) isolates gave positive result at 664 bp.In conclusion AgNps have an antibiofilm, antibacterial activity against P. aeruginosa and different bacterial species that could be used as an alternative therapy. In regard to pyocyanin production and silver resistance, phenotypically there was effect of pyocyanin pigment on silver resistance and the silver resistance was not due to carriage of silE, silS genes.

التشخيص والتوصيف الجزيئي لبكتريا Clostridium perfringens المعزوله من حالات مرضية سريرية في مدينة الحلة == Molecular Diagnosis and Characterization of Clostridium Perfringens from Clinical Samples in Al - Hilla city

Author name: علياء محمد حمود الشمري
Supervisor name: علاء هاني الجراخ | ميساء صالح الشكري
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: تهدف هذه الدراسة لمعرفة وبائية بكترياC. Perfringens في العينات المؤخوذة في محافظة بابل. تم جمع 140عينة من الجروح العميقة من المرضى الداخلين في مستشفيات مدينة الحلة الرئيسية (مستشفى الحله التعليمي ومدينة مرجان الطبيه) والذين يعانون من مرض السكري المزمن وايضا من مرضى تعرضوا الى طلق ناري فضلا عن المرضى الذين يعانون من التهاب الخلوي اللاهوائي في الجلد(anaerobic cellulitis ) للفترة من شهر شباط ولغاية شهرتشرين الاول 2016. بالاضافه الى ذلك تم تشخيص وعزل بكترياC. perfringens بطرق مختلفة منها استخدام الطرق البكتيرية التقليدية من خلال طرق التنمية على الاوساط الزرعية الاختيارية لهذه البكتريا واظهرت النتائج الحصول على 3 (2.14%)عزلات كان عزل اثنان منها 1.42%)) من مرضى السكري المزمن اما العزله الثالثه (0.72%) فقد تم الحصول عليها من الذين تعرضوا لطلق ناري في حين لم يتم الحصول على عزلات من المرضى الذين يعانون من الالتهاب الخلوي اللاهوائي . وكذلك تم تاكيد التشخيص باستخدام المعلم الوراثي المستند على دور جين 16s rRNA وجين16S - 23S spacer rRNA حيث اظهرت النتائج, من اصل 140 عزلة فان 7 عزلات تعود لبكتريا C. perfringens. بالاضافة الى ذلك, تم التحري عن قابلية هذه البكتريا واختبار قابليتها على انتاج انزيم (phpspholipase C) ووجد ان جميع العزلات منتجة الى هذا الانزيم. كما تم الكشف عن حساسية عزلات البكتريا اللاهوائية تجاه 24 مضادا حيويا. بينت نتائج الدراسة ان جميع العزلات اظهرت حساسية عالية (100%) تجاه كل من penicillins .مثل benzypencillin, ,amoxicillin فضلا عن حساسيتها تجاه  - lactamase inhibitor مثل Ampicillin - sulbactam وحساسيه عالية لكل من metronidazole وaminoglycosidesومن ناحية اخرى، اظهرت جميع العزلات مقاومة عالية لكل من مضادات tetracyclin, Linezolid, levofloxacin, erthromycin بينما اظهرت هذه العزلات تغاير في درجات المقاومة تجاه مضادات pipracillin, Clindamycin, and Impenem.تم استخدام الطرق الوراثية في الكشف عن جينات المقاومة للمضادات الحياتية كجيناتM,Q,W,B) ) tet الخاصة بالكشف عن المقاومة للتتراسايكلين وجينات erm(A,B) للكشف عن المقاومة للكلندامايسين وجين nim للكشف عن المقاومة للمترونيدازول حيث اظهرت النتائج ان الجين tet M كان ذا سيادة عالية بنسبة 71.42 %تم استخدام طريقة التشخيص الجزيئي لتوكسينات البكتريا (alpha,epsilon, iota) للاستدلال على وجود جينات (cpa, etx, iap)، في C. perfringens وبنسبة 100%, 28.4%, 28.4% )) على التوالي.بالاضافة الى ذلك تم استخدام جينات cpa, etx and iap لقياس تعاقب القواعد النيتروجينية للمادة الوراثية للعزلات المدروسة فضلا عن تسجيلها في بنك الجينات العالمي ( ,(Gene Bank,اذ تم خلال هذه الدراسة ولاول مرة في العراق، الحصول على خمسة ارقام انضمام الى بنك الجينات العالمي (5 accession numbers)، حيث تم نشر هذه الارقام في بنك الجينات الامريكي gene bank - NCBI وهذه الارقام هي : جينiap : KY523199, KY523200 اما فيما يخص الجينetx فكانت الارقام هي : KY523201, KY523202 وفيما يخص الجين cpaفكان رقم الانضمام هو : . KY523203تعد هذه الدراسه انها اول دراسه في العراق تضمنت تحليل , دراسه تعاقب القواعد النايتروجينية للمادة الوراثية فضلا عن التسجيل في بنك الجينات العالمي لسموم عزلات محلية من بكتريا C.perfringens معزولة من عينات سريرية. | This cross sectional and hospital - based study aimed to studying the incidenceof Clostridium perfringens in clinical samples in Babylon province. A total of 140 wound swabs were taken from patients admitted to two main hospitals in Hilla city, Iraq (Hilla Teaching Hospital and Medical Marjan City), during the period from February to October 2016. Swabs were collected from diabetic patients, anaerobic cellulitis, and bullet wounds. These wound swab samples were subjected to different methods for identification of C. perfringens according to standard method. It was found that 3 (2.14%) C. perfringens isolates were recovered, of which 2 isolates (1.42%) were obtained from diabetic patients, 1 isolates (0.72 %) from bullet wounds, No isolates are recovered from the other clinical samples (cellulitis). Furthermore, molecular detection method was applied by using 16s rRNA, 16S - 23S intergenic spacer rRNA genes as a genetic marker for confirmation of detection of C. perfringens isolates,7 isolates of C. perfringens out of 140 are detected by using PCR with specific primer.The study also focuses on the ability of this bacteria to produce Phospholipase C and it was found that all isolates are positive for this enzyme (100%) . The results of antibiotic susceptibility testing using Vitek 2 system and disc diffusion method (DDT) of C. perfringens isolates (No.= 3) against 24 antibiotics showed that all isolates were highly sensitive to penicillins, (benzylpenicillin, and amoxicillin), all isolates (3/3) were sensitive to  - lactamase inhibitor such as Ampicillin - sulbactum. They were more susceptible to metronidazole and aminoglycosides in (3/3). On the other hand, all isolates were highly resistant to tetracycline, Linezolid ,levofloxacin, and erythromycin However, these isolates expressed different degree of susceptibility towards other antibiotics (Clindamycin, piperacillin and Imipenem). Molecular detection of C. perfringens of antibiotic resistance genes had been investigated and it had been found that tetracycline resistance gene tet M is the most commonly observed (71.42%) among ribosomal protection genes among C. perfringens isolates. While all isolates (7) gave negative results for other tetracycline resistance genes (tet W and Q, tet B, erm(A), erm(B),nim gens).Furthermore, detection of clostridial toxin (alpha, epsilon, iota toxins was applied using specific genes (cpa, etx, iap) and the results showed that the rate of the presence of these genes are : 100%, 28.8% and 28.4% respectively. The following genes : cpa, etx and iap were used for sequencing analysis, registration in gene bank - NCBI. Five accession numbers were obtained from registration of five sequences of these three genes at gene bank - NCBI : iap gene; accession numbers are : KY523199, KY523200etx gene; accession numbers are : KY523201, KY523202cpa gene; accession number is : KY523203. This is the first study in Iraq which employed analysis sequencing and registration in gene bank - NCBI, of clostriaial toxin of local isolates of C. perfringens obtained from clinical samples

دراسة فيروس نظير الانفلونزا وبكتريا المسبحيات القيحية مع التهاب الاذن الوسطى القيحي في الاطفال == Study of Parainfluenza virus and Streptococcus pyogenes with suppurative otitis media in children

Author name: ايام محمد صالح علي العامود
Supervisor name: جواد كاظم طراد الخفاجي | غانم عبود المولى
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: هدفت هذه الدراسة الى التحري عن دور فيروس نظير الانفلونزا وبكتريا المسبحيات القيحية Streptococcus pyogenes في الاطفال الذين يعانون من التهاب الاذن الوسطى القيحي . جمعت 200 عينة (ثلاث مسحات لكل مريض تضمنت مسحة من افرازات الاذن ومسحة من افرازات الانف لدراسة الفيروس ومسحة من افرازات الاذن لدراسة البكتريا و50 عينة دم) من 50 مريض يعانون من التهاب الاذن الوسطى القيحي احيلوا الى مستشفى الحلة التعليمي في محافظة بابل للفترة من شباط (2016) لغاية ايار (2016). والذين يعانون من التهاب الاذن الوسطى. وتضمنت الدراسة 32 عينة ماخوذه من اشخاص اصحاء كمجموعة سيطرة وتم تقسيمهم الى ست مجاميع عمرية بنفس طريقة تقسيم مجاميع المرضى. شملت الدراسة اربعة اجزاء رئيسية : اولا التشخيص الفيروسي اعتمد على التقنيات المتقدمة (العزل الاولي باستخدام فحص التالق المناعي المباشر لتحديد وتمييز الانواع الثلاثة لفيروس نظير الانفلونزا 1,2, and 3 والتشخيص الجزيئي بواسطة استخدام فحص تفاعل سلسلة انزيم البلمرة بالوقت الحقيقي لتحديد وتمييز الانواع الاربعة للفيروس1,2,3, and 4 وتم استخدام المجهر الالكتروني النفاذ لمشاهدة جزيئة الفيروس . والجزء الثاني فحص البكتريا باستخدام فحوصات الكيموحيوية وفحص Vitek 2 system وتم التحري ايضا عن انزيم الهيمولايسين حيث كانت جميع العزلات منتجه لهذا الانزيم . التشخيص الجزيئي للكشف عن وجود الجين mga بواسطة تقنية سلسلة تفاعل انزيم البلمرة .الجزء الثالث شمل وجود الفيروس والبكتريا في عينات الاذن للمرضى المصابين بالتهاب الاذن الوسطى . الجزء الرابع تضمن دراسة مناعية لمعرفة دور الحركيات الخلوية IL - 6, TNF - α, IFN - α, CD8, and CD56))عند الاصابة بفيروس نظير الانفلونزا بواسطة فحص الامتصاصية المناعي المرتبط بالانزيم وباستخدام تقنية التالق المناعي المباشر لفيروس نظير الانفلونزا بانواعه الثلاثة (hPIV 1,2,3) كانت نسبة الاصابة 64% (اي 32 طفلا من اصل من اصل50) وعند استخدام فحص تفاعل سلسلة انزيم البلمرة بالوقت الحقيقي ( (RT - PCR وجد ان نسبة الاصابة بفيروس نظير الانفلونزا بانواعه الاربعة (hPIV_1,2,3 and 4) 44% ( اي 22 من 50) تضمنت الدراسة استخدام المجهر الالكتروني النفاذ لرؤية جزيئة فيروس نظير الانفلونزا في افرازات الاذن . اظهرت نتائج التشخيص البكتيري ان نسبة الاصابة بالمسبحيات القيحية هي 14%(7 من 50) بعد زرعها على وسط اكار الدم وحضنت لمدة 24 ساعة بدرجة 37 وتم فحصها بواسط المجهر الضوئي بعد تصبيغها بصبغة كرام , تم التشخيص بواسطة استخدام فحص Bacitracin sensitivity وفحص انتاج انزيم الهيمولايسين على وسط الدم بواسطة فحوصات الكيموحيوية وفحص Vitek 2 systemاظهر التشخيص الجزيئي للكشف عن وجود mga جين ان جميع العزلات تحتوي على هذا الجين اي نسبة 100% .كما اظهرت الدراسة ان نسبة وجود الفيروس مع البكتريا في في سوائل الاذن بنسبة (16%)( . 4 وكانت نسبة وجود الفيروس (72%) 18وشملت الدراسة ايضا فحص الحركيات الخلوية بواسطة فحص الامتصاصية المناعي المرتبط بالانزيم واظهرت النتائج ارتفاع مستوى السايتوكينات في مصل مرضى التهاب الاذن الوسطى مقارنة مع الاشخاص الاصحاء وكان اعلى مستوى لانترليوكين 6 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو) 88.24 )و (100.95) 1Pg/ml على التوالي . في حين كان اعلى مستوى لعامل النخر الورمي نوع الفا في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو (99.35 ) و(109.81 ) Pg/ml على التوالي. وكان اعلى تركيز لانترفيرون الفا في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو( 217.16) و(( 197.84 Pg/ml على التوالي . واظهرت النتائج ان تركيز الواسمين المناعيين CD8 وCD56 ان تركيزهما اعلى في مصل مرضى التهاب الاذن الوسطى مقارنة مع الاشخاص الاصحاء , اظهرت النتائج ان تركيز CD8 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو ((11.46±1.49 و(14.76±1.73) ng/ml على التوالي . في حين كان تركيز CD65 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو (12.82±1.62 )و (10.59±1.19) ng/ml على التوالي | This study aimed to detect the role of of parainfluenza virus and Streptococcus pyogenes in children suffering from suppurative otitis media. A total of two hundred sample (three swab for each patients include ear swabs , nasopharyngeal secretion for viral study and ear swab for bacterial study and 50blood sample) , were collected from 50 patients suffering from suppurative otitis media attending to AL - Hilla Teaching Hospital in Babylon - Iraq during the period from February 2016 to May 2016. The study includes 35 samples obtained from healthy subject as control group that classified to six group according to age in similar way of patient's with parainfluenza virus infection group years old The study includes four main parts .First one is the viral diagnosis based on relied diagnostic procedure ( Primary detection of parainfluenza virus in ear discharge by direct Immunofluorescence for parainfluenza virus 1, 2 and 3 types, molecular diagnosis by Real - Time PCR technique for the qualitative detection and differentiation of Parainfluenza Virus 1, 2, 3, 4 and diagnosis of parainfluenza virus by transmission electron microscope. The second part includes the bacterial diagnostic based on relied diagnosis procedure (using conventional biochemical reaction and commercial kit (Vitek 2 system). The heamolysis on blood agar was also observed in all isolates. Molecular detection of mga virulence gene for Streptococcus pyogenes was detected using conventional PCR technique. The third part is Co - infection between GAS and HPIV in OM specimens of children with suppurative OM. The fourth part is an immunological study to investigate the role of cytokines (IL - 6, TNF - α, IFN - α, CD8 and CD56) in HPIV infection by ELISA assay . Direct immunofluorescence for parainfluenza virus 1, 2 and 3 types antigen was positive in 64% (32 out of 50) , when we use Real - time reverse transcription PCR(RT - PCR) the percent of children infection with HPIV1, 2, 3, and 4 was 44% (22 out of 50). The study also include the use of transmission electron microscope for visualized the parainfluenza virus particle in ear swab. Streptococcus pyogenes isolates was detected in 14%(7 from 50) the isolate were confirmed after culturing on the blood agar and incubated for 24 hour at 37ºC, then microscopically by Grams̕̕ staining. Identification was done using commercially prepared bacitracin and the type of hemolysis on blood agar by primary biochemical test and confirmatory identification by vitek2 system compact. Molecular Identification of mga gene for Streptococcus pyogene showed that all Streptococcus pyogenes positive result 100%. The occurrence of Bacterial and viral co - infection in middle ear fluids which have been observed in 16% (4 out of 50 cases). HPIV was identified as a single agent in ear swab in 72%(18 out of 50). The study include estimation of some immunological parameter include (IL - 6, TNF - α, IFN - α , CD8 and CD56 ) using ELISA and revealed elevation the concentration of cytokines in sera of otitis media patients in comparison with healthy control groups. The concentration of IL - 6 is higher in sera of otitis media patients with parainfluenza virus than healthy control group. The high level of IL - 6 Pg/ml in otitis media patients for male and female was (88.24) and (100.91) Pg/ml respectively. Also level of TNF - α Pg/ml in otitis media patients for male and female was (99.3) and (109.8) Pg/ml respectively . The level of IFN - α Pg/ml in otitis media patients for male and female was (217.16 )and (197.84 )Pg/ml respectively. The results also show elevation concentration of CD8 and CD56 in sera of otitis media patients with parainfluenza virus than healthy control group. For CD8 ng/ml in otitis media patients for male and female was(11.46) and (14.76)ng/ml respectively. The level of CD56 ng/ml in otitis media patients for male and female was (12.82) and (10.59)ng/ml. respectively.

الكشف عن اللولبية البوابية في خزعة المعدة ودراسة مستوى P53 وطفرات مورثة Bax المرتبطة بالقرحة الهضمية == Detection of Helicobacter pylori in Gastric Biopsy and Study the Level of P53 and Bax Gene Mutations Associated With Peptic Ulcer

Author name: اصيل هاشم راضي السعبري
Supervisor name: محمد صبري عبد الرزاق السعيد | مشتاق عبد العظيم وتوت
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: اللولبية البوابية هي بكتريا سالبة لصبغة كرام, الذي تصيب غالبية سكان العالم وتسبب امراض مختلفة مثل التهاب المعدة المزمن, القرحة الهضمية وسرطان المعدة. في حين ان غالبية الناس المصابين بالبكتريا اللولبية لا تظهر عليهم اعراض مرضية. ان العوامل الرئيسية التي تحدد تطور الامراض ذات الصلة بالبكتريا اللولبية قد تكون ضراوة البكتريا, وعوامل المضيف الوراثية والبيئية.الهدف من هذه الدراسة هو الكشف عن اللولبية البوابية في انسجة المعدة ودراسة عوامل الضراوة الرئيسية لهذا الممرض والعوامل الوراثية للمضيف في المرضى الذين يعانون من اعراض اصابات المعدة والاثني عشري. وتشمل الطرق الجزيئية ايضا تفاعل سلسلة البلمرة (PCR) للكشف عن جينات التشخيص والضراوة لبكتريا اللولبية البوابية وتقنية (SSCP) تم القيام بها للكشف عن الطفرات الجينية ل P53 وال Bax جين التي قد تترافق مع عدوى اللولبية البوابية.بلغ العدد الكلي للنماذج (الخزعات) المعوية 180 نموذج. فقط 92 خزعة معدة (51 ٪) اعطى نتيجة ايجابية لللوليبيات البوابية (Helicobacter pylori ) للتشخيص بواسطة التقنية الجزيئية, مع ذلك من بين هذه النتائج الايجابية 60% منها اعطى نتيجة ايجابية للزراع, التي تعتمد على الميزات الكيميائية الحيوية. كما تم اخذ 100 عينة مصل الدم من افراد اصحاء مع عدم وجود تاريخ عدوى بالبكتريا اللولبية كمجموعة سيطرة ونتائج اختبار الاجسام المضادة على اساس (اختبار المصلية) اعطت نتائج سلبية للبكتريا اللولبية.قد تم ملاحظة وجود تكرار عالي للولبية البوابية بالتهاب المعدة المزمن الذي يشكل 88٪ بينما التكرار القليل كان مقداره 3٫3٪ لسرطان المعدة في حين ان 8٫7٪ كان لقرحة الاثنى عشري. وقد كان التكرار العالي لعدوى اللولبية البوابية بين المصابين الذكور اكثر من الاناث, ويعود سبب ذلك لعدة عوامل مثل (متوسط العمر الاجتماعي والاقتصادي ونقص التغذية وسوء النظافة) ويعتبر التدخين احد العوامل الاضافية المرتبطة مع انتشار عدوى (اللولبية البوابية ) بسبب السلوكيات غير الصحية المرافقة للتدخين , والتي قد تشمل نقل البكتيريا من خلال اللعاب بين المدخنين. من جهة اخرى اظهرت النتائج ان معدل العدوى العالي للمجموعة العمرية (31 - 50) سنة الذي تم تسجيله كان 44٫6٪. علاوة على ذلك فان الكشف عن اللولبية البوابية بتقنية سلسلة تفاعل البلمرة PCR)) قد اجريت على جميع انواع خزعات المعدة وجين (ure C ) اضافة الى جين (ure A ) اللذان يستخدمان كعلامات للتشخيص التاكيدي للولبية البوابية من خلال استخدام (primers ) خاص لتقنية PCR. وضحت النتائج ان 84٫8٪ و73٫9٪ للولبية البوابية كانت ايجابية لكلا الجينين. للعينات التي اعطت نتائج ايجابية (للتضاعف الجيني amplification ) بواسطة (PCR ), كان 56 عينة ما يمثل (60٪) قد اعطت استجابة لنتائج الزرع. اضافة الى التضاعف بواسطة PCR لعلامات جين (ure C ) وجين (ure A ). اضافة الى ذلك ,المعدل الايجابي للولبية البوابية للخزعات تحت الاختبار كان 51٪ بالاعتماد على الكشف الجزيئي المباشر بواسطة (PCR) باستخدام جين (ure A) وجين (ure C ). علاوة على ذلك, تم فحص جين (cag A) لكل عينات اللولبية البوابية التي تم عزلها. اشارت النتائج الى ان65 عزلة (70٫6٪) كانت ايجابية لتضاعف (PCR ) للجين من نوع cagA) ) حيث ان عزلة 56 (60٫8٪) كانت ايجابية لتضخيم (PCR ) للجين من نوع (cag E ). من بين النتائج الايجابية التي تم عزلها 49 عزلة (53٫3٪) كانت قد اعطت نتائج ايجابية للجين من نوع (cag A ). كذلك وجد تكرار الجين (vac A ) مقداره في الدراسة الحالية 68٫5٪ (63/92). اضافة الى كل اللولبيات البوابية الايجابية لخزعة المعدة 49 (53٫3٪) اظهرت اشارة كاملة للجينات نوع (cagA, cagE وvacA (. بالنسبة للعينات المعدية المتبقية 27 عينة (30٫4٪) التي تحوي سلالات سلبية كانت سلبية للجين (cag E). وقد تم تحديد طفرات جين (p53) في الاكسونات 5 و6و 7 و8 باستخدام طريقة SSCP. ومع ذلك تم استخدام PCR لتضخيم الاكسونات 5 و6 و7 و8 للجين (p53) التي من المعروف انها بقع ساخنة طافرة .تحليل PCR - SSCP يكشف تعديلات في الجين (p53) في عدة اكسونات. حيث ان ستة عشر من التعديلات في الاكسون E5A , سبعة عشر التعديلات في الاكسون E5B6A, وكذلك سبعة عشر التعديلات في الاكسون E7 وتعديل واحد فقط في الاكسون E6B لكن لم يظهرتعديل في الاكسون E8 في الجين (p53). بنفس الاسلوب ان تحليل PCR - SSCP كشف عن تعديلات في جين bax في الاكسونات E1 وE4 وE6. ويظهر ذلك في الاشكال. فيما يخص الاكسون 1 في bax يمكن ملاحظة وجود 6 نماذج فقط تعطي طفرات موضع ايجابية للجين. قد يعزى ذلك الى اللولبية البوابية التي تحفز طفرة bax من خلال قابليتها على انتاج بروتين cag A الذي له القابلية على تغيير التسلسل الجيني والوظيفة. واستنتجنا الى ان التشخيص الجزيئي قدم دليلا توكيديا على وجود اللولبية البوابية في عينات المعدة. ايضا قد تؤثر السلالات الاكثر ضراوة على تحفيز طفرات جينية في جينات ال p53 وال bax. | Helicobacter pylori is a gram - negative bacteria which infects a majority of the world population. It causes various diseases such as chronic gastritis, peptic ulcer and gastric cancer. While majority of the people infected with H. pylori is asymptomatic. The main factors, which determine the development of H. pylori related diseases might be bacterial virulence, host genetic and environmental factors.The aim of this study is detection of H. pylori in gastric tissue and study the main virulence factors of this pathogen and host genetic factors in patients with gasteroduodenal manifestation. Molecular methods include polymerase chain reaction (PCR) for diagnosis and virulence genes of H. pylori and single strand confirmation polymorphism (SSCP) technique was done to detect the p53 and bax genes mutation may be associated with H. pylori infection. In this study, 180 patient were included, only 92 gastric biopsies (51%) gave positive for the presence of H. pylori diagnosed by direct molecular technique. Among these positive result, 60% gave positive results in cultivation based on biochemical features. In addition, 100 serum samples were taken from healthy individuals, with no history of H. pylori infection as a control group and the results of antibody based test (serological test) were negative for H. pylori.In this study, the high frequencies of H. pylori were observed for chronic gastritis, which constituted 88% while low frequencies were 3.3% for gastric cancer, whereas 8.7% for duodenal ulcer.Also, the highest frequencies of H. pylori infection were found in male patients in comparison to female. On the other hand, the results showed that the high significant infection percentage 44.6%, among age group ranging from 31 - 50 years. Moreover, detection of H. pylori by Polymerase Chain Reaction (PCR) technique is performed on all gastric biopsy. ure C as well as ure A genes are used as markers for confirmatory diagnosis of H. pylori through using specific primers of PCR technique. The results show that 84.8% and 73.9% of H. pylori are positive for both genes, respectively. Among samples with positive result for PCR amplification, 56 samples (60%) also had positive culture result. Besides, the PCR amplification of ureC and ureA markers. Also, the rate of positive H. pylori in the tested biopsies was 51% depending on direct molecular detection by PCR using the ureA and the ure C genes.In addition, cag A gene is investigated in all H. pylori isolates. The results show that 65 isolates (70.6%) are positive for PCR amplification of cagA gene whereas 56 isolates (60.8%) are positive for PCR amplification of cag E gene. Among of these positive isolates, 49 isolates (53.3%) give positive result for cag A gene. The frequency of the vacA gene found in the present study is 68.5% (63/92).Furthermore, among of all H. pylori positive gastric biopsy, 49 (53.3%) reveal full signals of cagA, cagE, and vacA. The remaining 27 (30.4%) gastric biopsies, harboring cagA - negative strains, are PCR negative for cagE.Mutations of the p53 gene in exons (5,6,7 and 8) are identified by PCR - SSCP method. However, PCR is used to amplify exons 5,6,7 and 8 of p53 gene which are known to be mutational hot spots. PCR - SSCP analysis has detected alterations in the p53 gene in several exons. Where sixteen alterations in exon E5A, nineteen alterations in exon E5B6A, seventeen alterations in exon E7 and only two in exon E6B but no alteration is seen in exon E8 of P53 gene.In the same way, PCR - SSCP analysis detected alterations in Bax gene where only 17% of positive samples gave alteration in the sequence of Bax gene in exons E1, E4 and E6. Regarding exon 1 of Bax gene, it was seen that only 6 samples gave positive gene locus mutation. It conclude that the molecular diagnosis gave an evidence and confirmatory guide on the existence of H. pylori in gastric samples. Also the more virulent strain may be affected on the stimulation of p53 and bax genes mutation.

دراسة جزيئية وبكتريولوجية لبكتريا Porphyromonas gingivalis المعزوله من المرضى المصابين بامراض اللثه == Molecular and Bacteriological Study of Porphyromonas gingivalis Isolated from Patients with Periodontitis

Author name: فاطمة مالك عبود
Supervisor name: محمد صبري عبد الرزاق السعيد | الهام عباس بنيان
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: تم جمع 103عينة من الصفيحة الجرثومية العميقة (subgingival dental plaque) من المرضى الذين يعانون من التهاب اللثة المزمن والمتقدم (72, 31) على التوالي لمراجي العيادة الاستشارية لامراض اللثة في كلية طب الاسنان/ جامعة بابل والمركز التخصصي لطب الاسنان في مدينة الحلة. للفترة من كانون الاول 2013 الى حزيران 2014. تم تشخيص وعزل بكتريا P. gingivalis بطرق مختلفة منها استخدام الطرق البكتيرية التقليدية واظهرت النتائج ان 69.5%) 16) منها تم عزلها من التهاب اللثة المزمن وتم عزل 7 (30.5) من التهاب اللثة المتقدم باستخدام التنمية على الوسط الانتقائي الخاص لهذه البكتريا. وكذلك تم استخدام طريقة التشخيص الجزيئي باستعمال المعلم الوراثي المستند على دور جين 16s rRNA حيث اظهر ان12 عزلة من اصل 23 عزلة تم تشخيصها بشكل نهائي انها تعود لبكتريا P. gingivalis. منها حوالي 8 تعود الى التهاب اللثة المزمن و4 تعود الى التهاب اللثة المتقدم. بالاضافة الى ذلك, تم التحري عن قابلية هذه البكتريا للالتصاق(adherence ability) بالخلايا الطلائية المبطنة للفم حيث وجد ان جميع العزلات لها القابلية على الالتصاق بهذه الخلايا وتم ايضا اختبار قابليتها على انتاج انزيم (Gingpain) ووجد ان جميع العزلات منتجة الى هذا الانزيم.في هذه الدراسة تم التحري المظهري عن عملية تكوين الاغشية الحيوية(Biofilm formation) للعزلات المدروسة باستخدام فحص tissue culture plate test (TCP) اذ كانت مكونة للاغشية الحيوية بنسبة 100% وكذلك تم فحص تاثير بعض المواد المستخدمة في طب الاسنان على انتاج biofilm ووجد ان اعلى تثبيط سجل للكلورهكسدين (غسول الفم) والشب (Alum)ويليه تاثير Clove مقارنة باقل تاثير لمادة السعد ((Cyprus rotundus. وكذلك تم الكشف عن حساسية العزلات لبعض المضادات الحيوية ووجد بان كل العزلات كانت حساسة الى Co - amoxiclave وعلى العكس اغلب العزلات تظهر مقاومة عالية لل Amoxicillin بينما سجلت مقاومة قليلة جدا لل Ampicillinو Metronidazole(16.3%, 20.3%, ) على التوالي. واخيرا تم فحص تاثير بعض المواد المستخدمة في طب الاسنان على تثبيط نمو هذه البكتريا وتوضح النتائج ان اعلى تاثير سجل للكلورهكسدين (غسول الفم) والشب (Alum)ويليه تاثير القرنفل(Clove) mm) 25,30,40) على التوالي مقارنة باقل تاثير لمادة السعدC. rotundus (10mm) .في هذه الدراسة ايضا تم التحري عن التغاير الجيني ((TLR - 4 Polymorphisms باستخدام تقنية البلمرة والقطع بالانزيم ((PCR - RFLP. وتبين من النتائج وجود ثلاث انماط جينية في موقع طفرة Thr399Ile : CC وCT وTT ، اذ بلغت نسبة انتشارها ((12.5%,10%,77.5% في مجموعة المرضى على التوالي بينما هناك فقط نمطين في مجموعة السيطرة TT,CCوبنسبة انتشار(36.6% ,63.4%) على التوالي ، فيما بلغت نسبة انتشار الاليلC السائد في المرضى 82.5 % بالمقارنة للسيطرة 63.3% مع وجود اختلاف معنوي. وفي المقابل سجل وجود ثلاث انماط جينية في موقع طفرة Asp299Gly : AA وAG وGG وبنسبة انتشار % 67.5 و12.5% و20% في مجموعة المرضى على التوالي بينما هناك فقط نمطين في مجموعة السيطرة GG, AA وبنسبة انتشار34.4%) 66.6%,) على التوالي. حيث سجلت نسبة انتشار الاليل A السائد (73.75%) في مجموعة المرضى بالمقارنة بمجموعة السيطرة (66.6%) وبفرق معنوي. | In this study, 103 clinical samples were collected from subgingival dental plaque of patients with chronic (CP) and aggressive periodontitis (AP) (72 and 31) respectively, and (n=30) a healthy group admitted to teaching Hospital in college of Dentistry / Babylon University and specialized center of Dentistry in Hilla city, From February 2013 to June 2014). Out of which (n=40) with a chronic periodontitis group from them blood sample were collected for detection of gene polymorphisms in Toll - like receptor - 4 (TLR - 4). These dental plaque samples were subjected to different methods for identification of P. gingivalis mainly traditional bacteriological method. It was found that 23 P. gingivalis isolates were recovered by using selective media where 19 isolates (26.3%) obtained from (CP) and 4 isolates (12.5%) from (AP). Furthermore, molecular detection method was applied by using 16s rRNA gene as a genetic marker for confirmation of detection of P. gingivalis isolates, 12 isolates of P.gingivalis out of 23were detected by molecular method focusing on the role of 16s rRNA gene of P. gingivalis. 8 isolates were isolated from CP, and 4 isolates were isolated from AP subgingival plaques. In addition, these 12 isolates were investigated to detect Adherence of P.gingivalis to oral epithelial cells. The result showed that all isolates have ability to adhere to oral epithelial cells. The study also detected the ability of this bacteria for production of Gingipain, it was found that all isolates were positive for this enzyme at a rate 100%.Furthermore, biofilm formation was tested in the semi quantitative microtiter plate test. The results revealed that all isolates were biofilm former, high and moderate biofilm formation mode were accounted for (50%) while there are no isolates that express non biofilm formation.Regarding to effect of Chlorhexidine (CHX) gluconate (0.12%) on biofilm formation, the results of this study demonstrated (CHX) effectively and reduced the viability of biofilm - forming bacteria from moderate to 25%, weak to 75% and no strong. The study was also evaluating the effect of some plant extracts on biofilm formation. The results showed the highest effect for Alum potassium phosphate followed by Clove, in contrast to lowest effect of C. rotundus. And after screening the effect of Flagyl solution on production of bacterial pigment. The results showed that the metronidazole produced the highest inhibition activity range (30 - 10mm). Furthermore, the susceptibility of isolates to a variety of antibiotics had been investigated and it had been found that all isolates were sensitive to co - amoxiclave and, in contrast, the isolates showed high resistance to other type of beta lactam such as amoxicillin (54.3%). In addition, the result showed that the resistance of p. gingivalis was very low for ampicillin and metronidazole (16.3% and 20.3%), respectively.The screening of antimicrobial activity of (CHX) gluconate (0.12%) was carried out and the results showed that it produced the highest inhibition activity whether against p. gingivalis with inhibition zone range 40 - 15mm. Finally in vitro antibacterial activity of Alum, Clove and Cyperus rotundus plant extracts was studied and the results revealed that all tested isolates were inhibited by aqueous extracts at 50% concentration. The maximum inhibition zone was observed in Alum and Clove extracts respectively (30mm, 25mm), compared to the minimum inhibition by Cyperus rotundus (10mm). The antibacterial actions of 20% concentration of plant extract gave lower inhibition zone than 50% concentration of that extract which represented by (19mm, 16mm, and 10 mm) of the previous three mentioned extracts. This study also aimed to investigate the association of Thr399Ile and Asp299Gly single nucleotide polymorphisms (SNPs) in the (TLR - 4) with the incidence of (CP).The results revealed at the site of SNPThr399Ile, there were three genotypes for this SNP among CP patients; CC, CT and TT with frequency of 77.5%, 10% and 12.5 % respectively, whereas, there were only two genotypes among control group; CC and TT with frequency of 63.4% and 36.6% respectively with no significance differences between patients and control. However, the frequency of allele C (dominant) was higher among CP group (82.5%) than control (63.3) with significant difference. The site of SNP of Asp299Gly also showed three genotypes among CP patients; AA, AG and GG with frequency of 67.5%, 12.5% and 20% respectively, whereas, there were only two genotypes among control group; AA and GG respectively with frequency of 66.6 and 34.4% respectively with no significance differences between patients and control. However, the frequency of allele A (dominant) was higher among CP group 73.75 % than control 66.6%.

دور السايتوكينات المعزولة من انسجة المشيمة المغذية للجنين لدى النساء مع الاجهاض التلقائي == The role of cytokines isolated from feto - placental tissues in women with spontaneous miscarriage

Author name: ميسلون عبد الحميد ناصر
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Basrah
First pages:
Abstract: شملت الدراسة الحالية (172( امراة، تتراوح اعمارهم بين 16 - 41 عاما، من المرضى الذين قدموا الى مستشفى الولادة والاطفال في البصرة بسبب الاسقاط التلقائي غير الكامل في الشهور الثلاثة الاولى من الحمل ، بالاضافة الى النساء مع الحمل الطبيعي في وقت الولادة خلال الفترة من شباط 2012 الى ايار 2014 وتم تقسيمهن الى 3 مجموعات :  المجموعة - ا : وشملت )65) من النساء مع الاجهاض التلقائي المتكرر (RSM) خلال الاشهر الثلاثة الاولى، مع متوسط العمر (25.2 ± 7.28) سنة. المجموعة - ب : وشملت (36) من النساء مع الاسقاط التلقائي غير الكامل في الاشهر الثلاثة الاولى من الحمل ولها على الاقل ثلاثة مرات حمل طبيعي سابق (الاجهاض التلقائي غير المتكرر ( non - RSM بمتوسط من العمر (26.61 ± 6.97) سنة. المجموعة - ج : السيطرة (الحمل الطبيعي) : 71 امراة بمتوسط من العمر (26.17 ± 7.01) سنة. وتم الحصول على الدم الوريدي من المجاميع قيد الدراسة من اجل البحث عن المضاد المناعي م وج الخاص بمقوس قوندي , IgM ) IgG (. والتشخيص المصلي للاصابة بمقوسات قوندي يستخدم على نطاق واسع لفحص النساء الحوامل والمجهضات. كان معدل حدوث داء المقوسات (38.4٪) التي وجدت في 101 مجموعات الاجهاض التلقائي بواسطة فحص (ELISA). اظهرت عينتين (3.08٪) نتائج ايجابية المضاد المناعي (م). بينما كانت 24 (36.92٪) عينة ايجابية للمضاد المناعي (ج) لمقوس قوندي في مجموعات المرضى , بينما لم تسجل اصابة في 71 امراة من مجموعة السيطرة . غالبية النساء المصابة (50٪) في سن (30 - 39) سنة في حالة المضاد المناعي (ج)، في حين ان (10.26٪) في سن (20 - 29) سنة في حالة من المضاد المناعي (م).والنتائج كشفت عن ان اعلى نسبة للاجهاض التلقائي لوحظت في نساء الارياف . وفي الوقت نفسه، تم جمع نسيج الارومة المغذية للجنين خلال عملية الكشط والدم الوريدي للكشف وتقييم السيتوكينات المضادة للالتهابات (IL - 4، IL - 6، IL - 10) والسيتوكينات الموالية للالتهابات (IL - 2،عامل نخر الورم - الفا TNF - α، - γ IFN) وقياس تركيزها باستخدام فحص انزيم مرتبط البلمرة المناعي ، تبين تركيز عال (49.97 ± 8.82، 180.7 ± 15.03، 94.34 ± 7.68) للسيتوكينات في الارومة المغذية للجنين (IL - 4، IL - 6، IL - 10 على التوالي) في حالة مجموعة السيطرة بالمقارنة مع مجموعات المرضى وتم الكشف عن نتيجة عكسية في مصل الدم ، في حين ان التراكيز عالية من السيتوكينات الموالية للالتهابات (IL - 2، TNF - α، IFN - γ) كانت (134.17 ± 24.37، 432.16 ± 51.7، 100.46 ± 9.94 على التوالي) في حالة مجموعة الاجهاض التلقائي المتكررRSM)) بدلا من مجموعة السيطرة ومجموعة الاسقاط التلقائي غير المتكرر RSM - non مع وجود اختلافات معنوية كبير.p<0.001 وقد تبين في الدراسة الحالية وجود علاقة واضحة بين مناعة الامهات نوع TH2 والحمل الطبيعي، في حين يرتبط رد فعل جهاز المناعة نوع TH1 بفقدان الحمل. والتوازن الدقيق بين السيتوكينات الناتجة عن TH1 / TH2 ممكن ان يمنع خمج مقوسات قوندي . الذي يرتبط مع الانتاج الكبير من TH1 للسيتوكينات الموالية للالتهابات.وعلاوة على ذلك نتائج حساب مستويات CD44، اظهر فرقا معنويا عاليا p<0.001 في حال مقارنة بين مجموعة السيطرة والمجموعتين من النساء المجهضات . وهكذا تم تسجيل ارتباط CD44 مع فعالية خلايا TH2 ؛ لذا فمن المرجح انه قد يكون هناك وجود علاقة بين الحمل الطبيعي وكثافة CD44 على سطح الخلية

فاعلية البرنامج التثقيفي على معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة == Effectiveness of an Education Program on Health Care Workers' Knowledge and Practices toward Infection Control at Primary Health Care Centers in Holy Karbala City

Author name: سلمان حسين فارس الكريطي
Supervisor name: اركان بهلول ناجي
General topic: Medicine
Specific topic: Nursing
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: دراسة شبه تجريبية اجريت في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة للفترة من اذار 2102 . لغاية نهاية شهر نيسان 2102 لقد هدفت الدراسة الى تقييم فاعلية البرنامج التثقيفي على معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة. تم تقييم الاحتياج للبرنامج التثقيفي، واظهرت نتيجة التقييم وجود ضعف في معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية.) شملت عينة الدراسة ) 22 عاملا صحيا اختيروا بشكل غير عشوائي )عمدي( ، وقد قسمت العينة الى مجموعتين مجموعة الدراسة ) 22 ( عاملا صحيا، والمجموعة الضابطة ) 22 ( عاملا صحيا . تم بناء برنامج تثقيفي حسب احتياج العاملين الصحيين واعدت ثلاث استمارات واحدة للمعلومات الخاصة بالعينة لتحديد صفاتها، واستمارتين لتقييم معارف وممارسات العاملين الصحيين ، استعملت للاختبار القبلي والبعدي. تم تحقيق مصداقية الاداة بعرضها على مجموعة خبراء لمراجعتها وتحديد درجة مصداقيتها، وتم تحديد ثبات اداة القياس من خلال الدراسة التجريبية.تم الاختبار القبلي لافراد المجموعتين ومن ثم تطبيق البرنامج التثقيفي لمجموعة الدراسة فقط للفترة من التاسع عشر لغاية الثالث والعشرين من حزيران 2102 ، تم الاختبار البعدي الاول مباشرة بعد تنفيذ البرنامج، ثم الاختبار البعدي الثاني بعد اربعة اسابيع من الاختبار البعدي الاول.تم تحليل النتائج باستخدام الاحصاء الوصفي والاحصاء الاستدلالي. اظهرت النتائج استفادة العاملين الصحيين بعد تطبيق البرنامج التثقيفي حيث الزيادة في مستوى المعرفة والممارسة للعاملين الصحيين وان البرنامج التثقيفي الخاص بالسيطرة على العدوى كان فعالا في تحسين معارف وممارسات العاملين الصحيين المشاركين فيه وعدم وجود اختلافات هامة بين مستوى تحسين معارف وممارسات العاملين الصحيين نتيجة لتطبيق البرنامج طبقا للعمر، المستوى التعليمي، سنوات الخبرة والمشاركة في الدورات التدريبية في الاختبار البعدي لمجموعتي الدراسة والضابطة باستثناء سنوات الخبرة في مجموعة الدراسة فقد اظهرت النتائج وجود اختلافات هامة بين مستوى تحسين معارف العاملين الصحيين في الاختبار البعدي لمجموعة الدراسة .واوصت الدراسة بتوفير الادلة الارشادية والتاكيد على اهمية التعليم المستمر للعاملين الصحيين باستعمال الممارسات المعتمدة على الادلة لتطبيق الاحتياطات القياسية للسيطرة على العدوى. وان هنالك حاجة لاكثر من برنامج تثقيفي حول السيطرة على العدوى في كل مراكز الرعاية الصحية الاولية ولعينة اكبر لتستفيد من النتائج . | A quasi experimental study was carried out at the primary health care centers in holy Karbala city from March 2016 to the end of April 2017.The study aims to assess the effectiveness of an educational program on the knowledge and practices of healthcare workers in Karbala city. Assessment of needs was done to evaluate the needs for the educational program. The result of the assessment revealed that the healthcare workers had deficit knowledge and practice regarding infection control in primary health care centers. A Non - Probability sample of (52) healthcare workers was selected randomly and divided into two groups : (26) considered as a study group and the other (26) considered as control group. An educational program was constructed, and three formats were developed one for assessing demographic characteristics of sample, the other two for assessing healthcare workers knowledge and practice. Validity of the instrument was determined through content validity by a panel of experts, and reliability of the tool was determined through a pilot study.A pre - test was done for both groups; educational programs were applied only for the study group from June 19th to June 23rd 2016. Then the first post - test was applied after the implementation of the program and the follow up after four weeks from 1st post test.Analysis of data was performed through the application of descriptive and inferential statistics.The findings revealed that the healthcare workers of the study group had benefited from the implementation of the educational program; their knowledge and practice were significantly improved comparing with the control group, the infection control education program was found to be effective in knowledge and practices improvement to the participant HCWsand non - significant association between HCWs knowledge and practice improvement effect result by application of the education program and their age, education, years of experience and training session in post - test I for study and control group. Except for years of experience in study group it shows a significant association with knowledge improvement.The study recommends the availability of written guidelines and focus on the importance of continuing education for HCWs using the evidence - based practices for application of standard precautions of infection control; and more educational programs of infection control are needed in all PHC centers and on a larger sample to benefit from the results.

تقويم تحمل بعض اصناف البطاطا Solanum tuberosum L للملوحة خارج الجسم الحي == Evaluation of certain potato (Solanum tuberosum L.) cultivars for salinity tolerance in vitro

Author name: اماني اسماعيل خليل طاهر
Supervisor name: ماجد عبد الحميد ابراهيم | عواطف نعمة جري
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was carried out at the laboratory of plant tissue culture - college of Agriculture - University of Basra during the period 15/3/2013to 20/07/2015 ,to study the effect of salinity stress on some growthindicators contain in callus produced from three potato varieties (Lizeta,Arnova and Safari) in Vitro.The sprout of the three potato varieties (Lizeta, Arnova and Safari) were cultured in aseptic condition on Murashige and Skoog (MS) full strength media supplemented with IAA and BA at concentration of 1.0 mg / L in addition to GA3 at concentration of 0.1 mg / L.For callus induction, the shoots were cultured on (MS) media upplemented with 3 mg / L NAA in combination with 1 mg / L Kin or BA.Moreover; to study the effect of salinity stress on some growth indicators , the callus exposed to NaCl at concentration (0,80, 100, 120 ,140 and 160 mM ) four weeks period, in additon to the effect of salisalic acid in combination with NaCl on protien charectirstic in callus of three potato cultivars. The results sammurized at follow : 1 - The effect of cultivars and growth regulators results sammaurized : No significant on regeneriation ,but Arnova cultivar was superior in height plant and number of shoots reached (7.27 cm and 2.48 shoot ) respectively.Lizeta was superior in number of leaves (1.59 leaves / plant ), the combination of hormons (1.0 mg/L of eachIAA & BA) was superior in height plant and number of shoots reached (2.1 cm and 1.6 leaves / plant ) respectively. 2 - Lizeta cultivar was superior in (% callus induction, callus fresh weight on 28 and 45 days ,CHO,protein,proline, Lipid peroxidation and K+ ion) parameters reached (31% ,186.25 mg, 303.9 mg, 29.09 mg /gm dry weight , 16.29 mg/gm dry weight 3.05 μ.gm /dry weight , 204 nmol MDA g/ fw and 9.16 mg/gm dry weight ) respectively .While Ornova cultivar was superior in (plant hight,shoots number, callus dry weight ) reached (7.27 cm, 2.48 shoot/ plant and 18.13 mg) respectively .3 - The combination of (1.0 mg / l ) of BA and IAA affected significantly on number of shoots and leaves parameters and give (2.1 shoot/plant ,1.61 leaf/ plant ) respectivelywhile (1.0 mg/l IAA+0.1 mg/l GA3 +1.0 mg/l BA ) affected significantly on shoots hieght which reached 8.03 cm .4 - Varied response were found among potato callus growth under 100 mM concentration of salt stress, Arnova and Lizeta were superior as compare with Safari which didn’t show any response in addition to callus growth reduction were found at high concentration (120, 140 and 160 ) mM of salinity . Moreover,100 mM gave negative responce on on callus fresh and dry weight .Furthermore, NaCl affected significantly on V.C and Lipid peroxidation at high concentration of NaCl as compare to control treatment .Concerninig to (CHO,protien,proline, Na - 1 and Cl - 1 ions containt ) , 100 mM of NaCl gave posetive responce for both Lizeta and Arnova cultivars as compere to Safari which in turn recorded highest rate at 80 mM concentration and less concentration of protien , K and K/Na ratio 5 - the interaction of 120 mM NaCl with 0.250 mM SA affected significantly on callus growth in Lizeta as compare with other tow cultivars which recorded no responce NaCl affected posetively on Gen expression, in callus of potatocultivars which represented in a form of a new protein bands with new molecular weight at 80 and 100 mM in addition to the interaction of NaCl with SA treatment in Lizeta cultivar. 7 - Significant responce were found among NaCl and potato cultivars in % of shoot initiation reached 42.11% for Lizita in control treatment and less responce in 80 mM reached 11.78 %. 8 - Sucsessful % of acclimatized plant for Safari reached 82.59% as compare to other cultivars while Lizita was superior in plant hight number of leaves, total root system length ,total yield , number and dimeter of tubers reached (28.33cm, 5.50 leaf/plant ,1.06 dcm2 ,10.25 cm, 17.63 g/plant , 5.16 tuber/plant, 3.18 cm) respectively . On the other hand Safari cultivar were recorded significant reduction in proline, CHO, protien reached (2.03 μ.gm/dry weight, 23.05 and 5.77 mg/gm dry weight) respectively.

تشخيص فطري Trichoderma viride وTrichoderma harzianum بتقنية PCR واستخدامهما في تحسين القيمة الغذائية لمتبن واداء الحملان العرابية == Identification ofTrichodermaviride andTrichodermaharzianum by using Molecular Technique and Used to ImproveFeeding Value of Straw and Performance of Arabi Lambs

Author name: ميثم خلف علي الغالبي
Supervisor name: عماد فلاح حسن | طالب احمد جايد
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The objectives of current study were to identify Trichoderma viridi and Trichoderma harzianum by genetics methods and used them singly or both (3% for each treatment) in improvement of barley straw and animal performance. A total of 20Arabi lambs aged about 3 - 4months with an average weight of 22.725±1.43 kg distributed randomly and equally to four nutritional treatments. Lambs fed individually as 2% of body weight, while straw was given ad libtium. The results obtained can be summarized as follows : 1 - The study was able to identify TV and TH using three different PCR techniques, which are PCR - RAPD, PCR - ITS and PCR - IGS.TV and TH showed three and two bands respectively when using PCR - RAPD at nearly 600bp.While both fungi showed one band by using PCR - ITS and PCR - IGS and at nearly 600 and 2,400bp respectively.2 - Using both fungi (treatment 4) resulted in a significant improvement in the chemical composition of the straw through the high content of crude protein and low crude fiber, ADF and NDF compared to untreated straw.3 - There were improvements indigestibilityofcrude protein, fiber, cellulose, hemicellulose, ADFand NDF when the both fungi treatment was used. As well as total digestible nutrients of the fungus treated groups in comparison with untreated straw (control group).4 - Feed intake of treated straw groups increased significantly compared to untreated straw groupwiththe superiority of the group that was treated with both fungi (639.91 - 826.30 gm/head/day for control and both fungi group respectively). As well as there wasa significant improvement in feed conversion efficiency of a group treated with both fungi in comparison of other groups (6.38 - 5.52 kg feed/kg growth rate respectively).5 - Rumen pH, ammonia and total volatile fatty acid of lambs fed treated straw (6.22 - 6.89, 10.00 - 18.69; 11.35 - 13.61 for control115group and both fungi group respectively) were significantly higher than those of untreated group.6 - There were significant improvements in lambs’bodyweigh tsand their absolute and relative gains when fed treated straw (37.82 kg, 14.97kg and 65.67% respectively) especially the group fed both fungi in comparison with untreated group (32.79 kg, 10.03kg and 43.89%respectively).7 - Globulin, AST and ALT was not significantly influenced bytreated straw. On the contrary, there were a significant increase inhemoglobin, glucose, total protein, albumin and albumin/globulin ratio of the treated groupcompared with the untreated straw. While a significant decrease in urea and cholesterollevelin theblood serumof treated groups in comparison with untreated group.8 - Economic evaluation ofadding fungitostrawshowed improvement inthe relativeeconomic efficiencyby26.2%, 37.0% and 59.5% for adding TVorTH or bothtogetherrespectively. Adding fungi decreasedthe cost of feed from 46.65Dinars/ kg increase in body weight gains incase of untreated straw to 40.15dinars/ kg when adding both fungi.

دراسة بعض التغيرات المرافقة لتلقيح وعقد ثمار النخيل Phoenix dactylifera L صنفي الحلاوي والساير == study of some accompanying changes to pollination and fruit set of date palm Phoenixe dactylifera L.CV. Hillawi and Sayer

Author name: وسن فوزي فاضل عباس الابریسم
Supervisor name: مؤید فاضل عباس | ابتھاج حنظل التمیمي
General topic: Agricultural sciences
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was carried out during the growing season of 2014 and 2015 in a private orchard in Shatt Al - Arab with the objective of knowing the effect of pollination 1, 2, 4 and 8 day from opening of female spathes, source of pollen (Ghannami Akhder and Smasmii) and by passing pollination process by spraying unpollinated flowers with the growth regulators and ethephon on certain aspects of the physiology and anatomy of date palm fruits cvs. Hillawi and Sayer. The results may be summarized as follows : 1 - The date pollination had no effect on fruit growth curve, but had a significant effect on length of the khalal stage which was 22, 18, 16 and 15 days for the cv. Hillawi and 24, 20, 18 and 17 days for the cv. Sayer, for the pollination dates 1, 2, 4, and 8 from opening of female spathes. The fruits of Hillawi and Sayer cultivars entered the tamr stage after 19 and 20 weeks from pollination respectively.2 - Fruits produced from flowers pollinated 8 days from opening during the khalal stage was superior in all physical characteristics of the fruit, with the exception of seed weight, which had its highest weight during the first date of pollination, however therewere no significant differences in fruit for both growing seasons, fruits of the cv.Hillawi had the highest values of physical characteristics in cooperation with those of Sayer cv.3 - As for Chemical characteristics of the fruits, the fourth pollination date (8 days from flower opening) gave a significantly highest value of total soluble solids, total sugars and dry matter. The highest values for reducing sugars was recorded during the second date of pollination( 2 days after female spathes opening), fruits of athe cv. Hillawi had the highest values for all those chemical constituents with its highest value with fruit of Sayer cv. 4 - These was significant difference of the unpollinated flowers in free amino acid and soluble proteins content, The second date of pollination gave the highest concentration of free amino acids 3.269mg.g - 1, whereas the fourth pollination date gave the highest1.856mg.gP - 1 P . As for the date of sampling , the fruits record during the third date ( 5 days after pollination) had the highest concentration of both free amino acids and soluble proteins, fruits of the cv. Hillawi had a significantly high concentration of both chemical constituents in comparison with fruits of the Sayer cv.5 - The results showed, that there were significant difference in the concentration of both auxin and cytokinins of the flowers ،422.5 271.5, where the second date of pollination gave the highest concentration of both hormones in the flowers, the date of sampling after pollination, had no effect on the concentration of auxins. however, the third sampling date( 5 days from pollination) had the highest of cytokinins in young fruits cv. Hillawi had the highest values with fruit of Sayer cv. .6 - The fourth date of pollination had the highest percentage ripening whereas the second date of pollination gave the highest fruit set percentage bunch weight during the rutab stage. Fruits of the cv. Hillawi was superior with respect to percentage fruit set, percentage fruit ripening, bunch weight as compared with fruits of the cv. Sayer. As for the effect of pollen source7 - The pollen source had no effect on fruit growth curve, but had a significant effect on the length of the khalal stage, the length of the klalal stage for fruit produced by the pollen Ghannami Akhdar wasb18 and 21 days for fruits of the cvs.Hillawi and Sayer respectively, whereas the length of the khalal stage for fruits produced the Smasmii pollen was 21 and 25 days for both cultivars respectively.8 - Fruit pollinated by Ghannami Akhdar had the highest values for the physical characteristics, as compared with fruits produced by the pollen Smasmii the pollen source Ghannami Akhdar recorded.9 - the highest values for total soluble solids and dry matter, whereas the pollen source Smasmii gave the highest value for the water content and total acidity. Fruit of the cv. Hillawi had the highest value for those chemical constituents as compared with fruits of the cv. Sayer.10 - As for sugars, the increase in reducing and total sugars was slow during early stage of fruit growth, thereafter, there was a rapid increase in sugars which reached its highest values during the 18 the week from pollination. For sucrose, its content in the fruit was at its highest during the 16 th week from pollination. Fruit produced by the pollen source Ghannami Akhdar had a significantly higher content of reducing and total sugars as well as sucrose. Fruits of the cv. Hillawi had a significantly higher sugars as compared with fruits of the Sayer cultivar.11 - As for changes in the activity of the enzymes invertase and cellulose the activity was low during early stage of fruit growth, and the highest level of activity was recorded during the 16 th week from pollination for both enzymes, for fruits of both cultivars. However, the activity of both invertase and cellulose was high for fruits of the Hillawi cv.2093, 787.7 As compared with fruits Sayer.12 - As for auxins, the concentration was in unpollinated female flowers. Fruits produced by the pollen Ghannami Akhdar had the highest concentration of auxins397.3 as compared with fruitscproduced by the pollen source Smasmii, the fifth sampling date( 7 days from pollination) gave the highest auxin concentration of young fruits. The unpollinated flowers of the cv. Hillawi had a significantly higher concentration of auxins as compared with those of the Sayer cv.13 - The study of embryogenesis of female flowers have shown that the process of fertilization took place after 2 days for flowers of Hillawi and Sayer pollinated by the pollen source Ghannami Akhdar, whereas fertilization took place after three days for flowers pollinated with pollen source Smasmii. However, the cv. Hillawi was superior in those characters as compared with the sayer cv.14 - The pollen source also, had a significant effect on percentage fruit set and fruit ripening, bunch weight as compared with fruits produced by the pollen Smasmii. However, fruits of the cv. Hillawi was a significantly superior, in those characteristics.15 - As for the by passing of the process of pollination by treating unpollinated flower with gibberellic acid and ethephon, such treatments had no effect on fruit growth curve, for both cultivars.16 - As for anatomical characteristic of parthenocarpic fruits not treated with gibberellic acid and ethephon(control), it gave the highest number of layers of exocarp, hypodermis and stone cells. The seeded fruits had the highest thickness of the inner mesocarp. Fruits of the cv. Sayer was superior with respect to thickness of the exocarp cells, hypodermis and stone cells, outer and inner mesocarp and tannin cells 259.6, 117.9, 117.8, 810.0, 3734, 773 μm Also, there were a change in the number as the Sayer cv 630.0mmPP. Had the highest number of cells as compared with fruits of the cv.Hillawi. The Hillawi showed the highest width of cells 72.5 μm, as compared with the cv. Sayer, but there were no difference in cell length. The control treatment gave the highest number of cells in pith tissues of parthenocarpic fruit, whereas the seeded fruit gave the highest length and width of cells.17 - treatment with gibberellic acid and ethephon had a significant effect on chemical characteristics of the parthenocarpic fruits treatment with 200mg.l - 1 +1000mg.l - 1 ethephon, caused a significant increase in total sugars and reducing, total soluble solids, and ripening percentage 56.09, 47.97, 59.09, 23.09% for parthenocarpic fruits of both cultivars Hillawi and Sayer. e

تاثير حقن بيض التفقيس ببعض المغذيات والتغذية المبكرة في بعض الصفات الانتاجية والفسيولوجية لفروج اللحم == The effect of in ovo - injection of Hatching Eggs with Some Nutrients and early Feeding on Productive Performance and Some Physiological Traits of Broiler Chicks

Author name: هدى فالح سعد الساعدي
Supervisor name: رياض كاظم موسى | طارق فرج شوكت
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was undertaken at the poultry field of Animal ResourceDepartment \college of Agriculture \University of Basra in Jan.,28th toMar.,3th,2013 and in Oct.,28th to December,23th 2013 for the first and secondexperimentrespectively . The objective of this study was to evaluate the effectof hatching eggs injection with the some nutritious (Folic acid vitamins,Pyridoxine, Threonine amino acid) for the first experiment and choosing besttreatment from first experiment and injection with Arginine amino acid ,folicacid as well as the effect of feeding time on the performance and somephysiological traits of broiler chicks .In the first experiment ,825 hatching eggsof Ross 308 broiler were received from the hatchery .At 14th day of incubationstage ,eggs were tested and unfertile eggs were discarded ,the remainder eggswere divided into seven treatments and injected with 0.1 ml of solution in airsac position of the egg as follows : 1. First treatment : injection 100 eggs with 0.1ml of threonine solution (30μgconcentration per an egg).2. Second treatment : injection 100 eggs with 0.1 ml of b6 vitamin solution(100μg concentration per an egg)3. Thirdtreatment : injection 100 eggs with 0.1 ml of mix from L - threonine aminoacid plus b6 vitamin (30μgand 100μg) per an egg.4. Fourthtreatment : injection 100 eggs with 0.1 ml of Folic acid vitamin solution(250g) per of an egg.5. Fifthtreatment : injection 100 eggs with 0.1 ml of mix solution from Lthreonineamino acid plus Folic acid vitamin (30μg and 250 g) per an egg.6. Sixthtreatment : positiveControl : injection100 eggs with 0.1 mlSterile distilledwaterper egg.7. Seventhtreatment : negativeControl : 155 eggs were left withoutinjection.Hatching chicks of each treatment were distributed in to three replicate (15 Chicksfor each replicate) .Birds were reared up to five weeks of age.The results of first experiment painted out to : 1. There was significantly (P<0.05) proceeding for the treatment of egg injectionwith some nutrients on hatching percentage ,hatching chick weight ,bodyBweight ,body weight gain and weekly and Cumulative feed conversion ratio.whereas, this treatment showed less embryonic mortality percentage incomparison with other experimental treatments and There was significantly(P<0.05) increase in weekly and Cumulative feed intake of birds that werehatched from injected eggs with ( folic acid ,threonine amino acid) during thesecond ,fourth and fifth week were as ,the treatment of pyridoxine injectionshowed less mortality percentage compared to other experimental treatments .2. Treatment of folic acid and threonine and pyridoxine injection showedsignificant (P<0.05) increases in the dressingpercentage, weights of breast,thigh and edible viscera comparison with other treatments.3. There was significantly(P<0.05) increase in red blood cells number,hemoglobinconcentration package cells volume of broilers that were hatchedfrom egg injection with (folic acid plus threonine) at 3 and 5 weeks of agecompared to other treatment While , Treatment of Pyridoxine eggs injectionshowed highest mean of white blood cells at 3 weeks of age, whereas,treatment of Pyridoxine and folic acid eggs injection proceeding otherexperimental treatment in highest mean of white blood cells at 5 weeks of age .4. There was significantly(P<0.05) increase in total protein concentration,glucose ,Globulins, and less uric acid concentration and cholesterol in chicksblood serum of folic acid and threonine egg injection treatment at 3 and 5weeks of age in comparison with other treatment. Whereas, there were nosignificant effect of egg injection on triglycerides concentration and on T3 andT4 hormone in broiler blood serum.5. There was no significantly effect of egg injection with nutritive on low densityfatty proteins concentration whereas,the negativecontrol treatment showedhighest concentration of low and high proteins at 3 and 5 weeks of agecompared to other experimental treatments.6. T4 hormone concentration in hatchingbroiler blood serum of threonineinjection treatment increased significant (P<0.05) at 3 weeks of age ,whereas,T4 hormones concentration was higher respecting treatment of folic acid andthreonine injection at 5 weeks of age in comparison with other treatment.7. There was significantly (P<0.05) increase in phosphorus ,calcium and ferricconcentrations in blood serum of the hatchingbroilers of folic acid andthreonine egg injection treatment at 3 and 5 weeks of age in comparison withother treatments.C8. There was significantly (P<0.05) increase in the villus lengths cryptsdepth ofjejunum of the boilers Caracasrespecting Folicacid and Threonine injectiontreatment in comparison with other treatment.According to the firstexperiment results and choosing the best treatment of egginjection with nutritive, the second experiment was carried out .the treatmentof this experiment whereas follows : 1. Firsttreatment : hatching eggs injection with (30μg of threonine, 250g of folicacid) per an egg.2. Secondtreatment : hatching eggs injection with (5 μg of arginine) per an egg.3. Thirdtreatment : hatching eggs injection with (5 μg of arginine plus 250 goffolic acid) per an egg.4. Fourth treatment : the control treatment (withoutinjection).Hatching chicks of each treatment were divided in to groups each of threereplicates ( 12 Chicks for each replicates ) .firstgroups chicks were fed at thehatchery ,whereas,second groupschicks were fed after 24 hours at field. Chickswere reared from 1 day after hatching up to 5 weeks of age .the results of thesecond experiment pointed out to : 1. Treatments of injection with arginine and Folic acid and threonine plus folicacid proceeding significantly (P<0.05) in hatching percentage andhatchingchicksweight, whereas, This treatment showed less percentage of lateembryonic mortality in comparison with other treatments. Also, Broiler birdsof Arginine and Folic acid injection treatment showed significantly (P˂0.05)increase in body weight, weekly body again, weekly and Cumulativefeedintake,weekly and Cumulative feed conversion ratio and dressing percentage.Whereas, treatment with arginine and folic acid injection showed less totalmortality mean compared to other treatments. Also, there was significantly(P˂0.05) effect of feeding time on some productive traits. Treatments offeeding at the hatchery showed significant increase in body weight, bodyweight gain, feed intake, better feed conversion ratio and dressing percentagein comparison with treatments of feeding at field after 24 hours.2 - There was a significantly (P˂0.05) effect of interaction between eggs injectionwith nutritious and feeding time on body weight, body weight gain and feedconversion ratio. Treatment of folic acid arginine (hatchery feeding) showedDhighest body weight, body weight gain, better feed conversion ratio incomparison with the control treatment (Field Feeding after 24 hours).Treatment of arginine injection (HatcheryFeeding) was highest dressingpercentage, whereas there was no significant effect of interaction on feedintake and total mortality.3 - Treatment of threonine and folic acid injection showed a significantly (P˂0.05)increase in bird’s body fullness, gizzard weight and Fabricia gland. Whereas,treatment of arginine and folic acid injection showed a significantly (P˂0.05)increase in breast fullness liver and heart weight in comparison with othertreatments.4 - Feeding time affected significantly (P˂0.05) on some carcasses traits. Carcassesof feeding at hatchery treatment were better significantly in body fullness,breastfullness, and heart, liver, gizzard, and Fabricia gland and pancreasweights. Whereas, carcasses of feeding at field treatment were of high pad fatweight. There was no significant effect of interaction between injection andfeeding time on carcasses traits.5 - There was a significant (P˂0.05) increase in red and white blood cells numbers 3days and 5 weeks of age and in hemoglobin concentration at 5 weeks of agerespecting the treatment of arginine and folic acid in comparison with othertreatments. Control treatment exceeded in Heterophil cells\ lymphocyte cellspercentage at 3 and 5 weeks of age compared to treatments.6 - There was a significantly (P˂0.05) effect of feeding time on some cellular bloodcharacteristics. Treatment of the field feeding exceeded in red blood cellsnumbers, hemoglobin concentration and Heterophil cells\lymphocyte cellspercentage at 3 days and 5 weeks of age. Whereas, treatment of the hatcheryfeeding exceeded in white blood cells numbers compared to treatment of thefield feeding after 24 hours.There was no significantly (P˂0.05) effect ofinteraction between injection and feeding time on the cellular bloodcharacteristics at 3days of age, whereas this interaction affected significantly(P˂0.05) on the white blood numbers and Heterophil cells\lymphocyte cellspercentage at 5weeks of age.E7 - Treatment of arginine and folic acid injection exceeded significantly (P˂0.05)in serum total protein concentration at 3 days and in total proteinconcentration, uric acid and GPT, GOT enzymes in blood plasma at 5 weeksof age. Treatment of threonine and folic acid in comparison with othertreatments.8 - Feeding time affected significantly (P˂0.05) on biochemical bloodcharacteristics. Treatment of the hatchery feeding exceeded in glucoseconcentration and total protein at 3 days of age and in total protein concentration and uric acid at 5 weeks of age. Treatment of the field feeding exceeded in blood serum cholesterol concentration in comparison with thetreatment of hatchery feeding. There was no significant (P˂0.05) effect ofinteraction between injection and feeding time on the biochemical bloodcharacteristics at 5 weeks of age. 9 - There was a significant (P˂0.05) increase in villus Length and crypts Depth of the intestinal jejunum of comparison with of threonine and folic acid injection treatment in comparison with other treatments. Treatment of the hatchery feeding exceeded (P˂0.05) respecting those traits compared to the field eeding treatment. There was a significantly (P˂0.05) effect of interaction (TheHatchery Feeding) and feeding time villus, length and crypts Depths werehighest at 1 and 5 weeks of age.

تاثير الرش بالسليكون في تحسين التحمل الملحي لصنفين من الطماطة Lycopersicon esculentum Mill المحدودة النمو == Effect of spray silicon on salinity tolerance improvment of two tomato (Lycopersicon esculentum Mill.) cultivars growth limited

Author name: ناصر جبير راضي حسين الحسناوي
Supervisor name: مؤيد فاضل عباس | عواطف نعمة جري
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This experiment was conducted during two growing season (2015 and 2016) in private farm in Kufa - Najaf, with the objective of improving salinity tolerance of tomato (Lycopersicon esculentum Mill.) with application of silicon. The study included 40 treatments, while were four levels of irrigation water salinity (2.5, 5.0, 7.5 and 10 dS.m - 1) , two tomato cultivars (Aulla and Majid) and five concentration of silicon (0 , 50 , 100 , 150 , and 200 mg l - 1). A split - split plot design was used in a factorial experiment with three factors and three replicates. The results were analyzed using analysis of variance and treatment means were compared using the least significant differences (LSD) at 0.05.The effect of the above treatment were studied on vegetative growth parameters, chemical constituents of leaves, flowering parameters ,yield and yield components, as well as fruit chemical parameters. The results may be summarized as follows : 1 - vegetative growth parameters : Irrigation water salinity at 5, 7.5 and 10.0 dSm - 1 caused a significant decrease in plant length ,number of side branches, leaf number, leaf area as well as fresh and dry weights of the shoot system, for both growing seasons. Treatment with silicon caused a significant increase in all vegetative growth parameters, for both growing seasons. As for the effect of cultivars, the cultivar Majid was superior in all vegetative growth parameters, as compared with cv. Aulla, for both growing seasons. As for interactions among treatments, they were significant in their effects on vegetative growth parameters and for both seasons.2 - chemical constituents of leaves : Irrigation water salinity treatments at 7.5 and 10.0 dS m - 1 caused a significant increase in the concentration of sodium and chloride ions, proline, as well as the plant hormone ABA and potassium ion leakage. However, such treatments caused a significant decrease in K+, Ca++and Si ions total solute carbohydrates and total chlorophylls, and the concentration of both auxins and gibberellins and K+ : Na+ ratio.As for treatment with silicon, at 200 mg l - 1 caused a significant increase in total soluble carbohydrates, total chlorophylls, auxins , gibberellins as well as K+,Na+ and Si ions and for both growing seasons. However, the sane treatment led to a significant decreased in the concentration of Na+ and Cl - ions as well asbfree proline concentration, and ABA levels. Treatment with silicon al so caused a significant increase in K+ : Na+ ratio, but decreased potassium ion leakage. As for the effect of cultivar plants of the cv. Majid had a significantly higher levels of most chemical constituents as compared with plants of the cv. Aulla, with the exception of the concentration of Na+, Cl - , ABA and gibberellins wheres plants of the cv. Majid had the higher levels. As for interactions among treatments, they were significant as there was an increase in the concentration of K+, Si , total soluble carbohydrates, total chlorophylls, auxins , gibberellins and K+ : Na+ ratio for both growing seasons.3 - flowering parameters, yield and yield components : Irrigation water salinity at 7.5 and 10.0 dS.m - 1 a significantly reduced the number of inflorescence, total number of flowers per inflorescence as well as percentage fruit set. This treatments also caused a significant reduction in yield components, represented by number of fruit, mean fruit weight, yield per plant and total yield for both growing seasons. Silcon treatment at 50 ,100,150,200 mg L - 1 significantly increased total marketable yield as much as (18.16, 25.12, 37.18 , 35.82) for first season and (19.26, 30.05, 35.83, 29.09) as compared with untreated treatment for second season, respectively. For cultivars, the cultivar Majid was significantly superior in total marketable yield as much as (19.65, 20.66 and %), as compared with cv. Aulla for both growing seasons respectively. The interactions among treatments, were significant in increasing flowering growth parameters, as well as yield and its components.4 - fruit chemical parameters : Irrigation water salinity at 7.5 and 10.0 dS.m - 1, caused a significant reduction in ascorbic acid concentration and concentration of β - Carotene and Lycopene, it caused a significant increase in total soluble solids, titratable acidity and, percentage dry matter, as well as the. As for application of silicon, it caused a significant increase in all chemical parameters. The cultivar Majid had a significantly higher chemical constituents as compared with the cultivar, Aulla. The interactions among treatments, were significant in increasing the chemical constituents of the fruits.

تاثير هيومات البوتاسيوم والكبريت في صفات التربة والنمو والحاصل وخزن الطماطة المزروعة تحت الانفاق البالستيكية في المنطقة الصحراوية == EFFECT OF POTASSIUM HUMATE AND SULPHUR ON CHARACTERISTICS OF SOIL , GROWTH, YIELD AND STORABILITY OF TOMATO (Lycopirscon esculentum MILL.) CUITURED UNDER PLASTIC IN DESERT REGION OUTHERN IRAQ

Author name: ميسون موسى كاظم محمد الساعدي
Supervisor name: عبد الله عبد العزيز عبد الله
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present experiment was carried out during two winter grawing seasons (2013 - 2014 and 2014 - 2015) in one of the dry land farms,khor AL - Zubiar Basrah with the objective of studying.The response of the Hybrid tomato (Lycoperiscon esculentum mill.) CV.Hitouf grown under plast poly ethyien to varins fertilizer treatment of organic fertilizer pow humus and inorganic fertilizer Agriculture sulphur .to study of the effect of the fertilizer treatment was on soil physical characteristics,vegetative growth characteristics,flowering and leaf chemical constituents wells quantitative and and quantitative yield ,and storability of behavior of the fruit at room temperature or cold storage .The experiment included nine treatments in afactorial mannes which were the combination of three levels of potassium humate (0 ,255 ,510 kg K.haˉˡ) and three levels of Agricultural sulfur (0 ,205 ,510 kg S. .haˉˡ) the experiment was performeal as factorial with completely randomized block design (CRBD)with three replicater Mean treatment value ware compared using least significant L.S.D at 0.05 level.The results may be sumuarized as follows : 1 - phsical and chemical soil characteristics .The addition of potassium humate at 255 and 510 kg K.hˉˡ caused significant increase in soil water content as compared with control treatment during the second growing season ,wheres the level of 255 kg k.ˉˡ caused asignificant decrease in soil pH as compared with control treatment as well as the level 510 kg K.haˉˡ humate during the first growing season caused asignificant decrease in soil pH,and Ec soil electrical conductivity for both growing seasons,and asignificant in lreane in soil moisture contant the yield growing season .For sulphur treatment ,it was fourd that the addition of Agricultural sulphur at 205 and410 kg S.haˉˡ caused asignificant decrease in soil pH for both growing seasons and a significant increase in soil moisture contant for both seasons as compared with control treatment.However ,sulphur at 500However,sulphur at 410 kg S.haˉˡ caused asignificant decrease in soil Ec during the secand growing.The time period caused asignificant decrease in soil pH and Ec soil during both seasons wheres soil moisture contant increased significant as the time period increased ,2 - Vegetative growth characteristics The addition of K humate at 255 and 510 kg K.haˉˡ caused a significant increase in plant length and number of side shoots during both growing seasons ,as compared with control treatment . Those two levels of K humate also caused asignificant increase in stem diameter ,total leaf number and both fresh and dry weight of the plant during the secand of growing season.However ,K - humate at 72 kg .haˉˡ caused asginificant increase in leaf area during both growing season ,where the increase in total leaf number and fresh weight of the plant was for the first growing season only.Sulphur at 205 and 410 kg S.haˉˡ caused a significant increase in plant length ,leaf area and plant dry weight during both growing season whereas the levels of Khumate had a significant effect with respect to plant from weight during the first growing season ,and total leaf number during the secand growing season.However ,the addition of Khumate at 410 kg S.haˉˡ induced asignificant increase in leaf number of side shoots during the first growing seaeon as compared with the level 205 kg S.haˉˡ and control treatment .The level 410kg S.haˉˡ caused asignificant increase in plant fresh weightduring the second growing season as compared with the level 205 kg S.haˉˡ .3 - flowering characteristics.The addition of K - humate of 255 and 510 kg K.haˉˡ caused a significant increase in number of inflorescens ,number of flowers per inflorescens during both growing season compared with control treatments .However ,both levels reducal the percentage of flower abortion during the first growing season .At 510 kg K.haˉˡ percentage flower decreased as compared with 36 kg K.haˉˡ and control treatment. Sulphur treatment at 205 and 410 kg S.haˉˡ reduced the percentage of flower aboration significant as compared with control treatment during the first growing season.At 410 kg S.haˉˡ ,sulphur ,that was a significant increase in number of inflorescane as compared with contror during the first growing season .cowever ,sulphur had no significant effect on number of flowers per inflorescence during both growing seasons .The interaction treatments had no significant effects ,with the exception of percentage flower aboration during the first growing season.K humate at 510 kg K.haˉˡ caused asignificant decrese in percentage flower abortion as compared with K humate at 255 kg K .haˉˡ during the secand growing season.The addition of sulphur at 205 and 410 kg S.haˉˡ reduced the percentage of flower abortion significantly as compared with control treatment during the first growing season.However, sulphur at 410 kg S.haˉˡ increased significantly the number of inflorescence as compared with control duringthe first growing season .Sulphur had no significant effect on flower number prr inflorescence for both seasons.4 - Leaf chemical constituents.The addition of K humate at both levels(255 and 510 kg K.haˉˡ ) caused a significant increase in total soluble carbohydrates,K+/ Na+ ratio and a significant decrease in chloride ion during both growing season.There was also a significant in total chlorophylls, proline, and percentage of N,P,K,S,Mg and Ca during the first growing season only.There was a significant increase the percentage of K+ and adecrease in Na+ ion during the second growing season .K humate at 510 kg K.haˉˡ caused asignificant increase in total chlorophylls and proline and percentage of S and Ca during second growing season.Plantfertilized with sulphur at 205 and 410 kg S.haˉˡ had asignificantly higher concentrations of total soluble carbohydrates ,total chlorophylls and percentage of N,P,S,Mg during both growing seasons as compared with control plants such treatments also caused a significant increase in percentage K+ ion,K+/Na+ ratio during the first growing season.However ,proline content,Ca++ ion was significantly increased, wheras chloride ion (CLˉ) was significantly decreased due to sulphur fertilization,during the second growing season .Sulphur at 410 kg S.hˉˡ caused a significant increase in percentage of Ca++ during the first growing season,and K+ ion during both growing season in comparison with 250 kg S.hˉˡand control treatment.5 - Quantitative yield.Plant fertilized with K humate at 255 and 510 kg S.haˉˡ had a significantly higher average fruit weight during both growing seasons ,fruit number and plant yield and total yield during the first growing season and early yield during the second growing season however supplying K humate at 510 kg K.haˉˡ had a significantly higher early yield during the first growing season and in fruit number ,plant yield and total yield during the second growing season.As for fertilization with agricultural sulphur, plant fertilized with 250 and 410 kg S.haˉˡ had a significant higher fruit weight as compared with control treatment during both season fruit number ,plant yield during the first growing season and early yield during the second growing season .Sulphur fertilizer at 410 kg S.haˉˡ had a significantly higher fruit number,plant yield and early and total yield during the second growing season compared with control treatment.There was a significant interaction between studied factors in certain characteristics and both growing seasons.6 - Qualitative yield.Tomato plants fertilized with K humate at 255kg K.haˉˡ had a significantly higher total titratable acidity percentage during both growing seasons ,vitamin C contant during the first growing season ,and percentage dry matter during the second growing season in comparion with control treatment.Plants fertilized with 510 kg S.haˉˡ had a significantly higher total soluble solids content during both growing season and in percentage dry matter during the first growing season and vitamin Ccontent during the second growing season.Plant fertilized with agricultural sulphur at both levels (205 and 410 kg S.haˉˡ )had asignificantly highr total titratable acidity during both season asecompared with control treatment, and percentage matter during the second growing season.Plants fertilized with sulphur at 410 kg S.haˉˡ had a significantly higher percentage dry matter and vitamin C content during second growing season.7 - Storage behavior.A - Storage at room temperature 25±1c .Storage at room temperature percentage fruit decay and loss in weight decreased significantly for fruit fertilized with K humate at levels of 36 and 510 kg K.haˉˡ ,and there was an increase in total soluble solids.Also,fruit decay percentage significantly decreased and total soluble solids increased in fruits of plants fertilized with sulphur at 205 and 410 kg S.haˉˡ during both growing seasons,and adecrease in weight loss during second growing season ,however,sulphur at 410kgS.haˉˡ caused a significant decrease in percentage season.Percentage fruit decay ,weight loss and total soluble solids increased ,with increasing storage period during both growing season .However vitamin C content decreased with increasing storage period .B - The cold storage 5±1 c.Both percentage fruit decay and percentage loss in weight decreased whereas total soluble content increased in fruit of plants fertilized with potassium humate at the levels of 255and 510 kg K.haˉˡ significantly as compared with fruit of control plants and for both growing seasons .Also,the percentage loss in weight for fruits of plants fertilized with agricultural sulphur at 205 and 410 kg K.haˉˡ ,decreased significantly for both growing seasons.Percentage fruit decay decreased significantly during the first growing season as well as an increase in total soluble solids during the first growing season.However sulphur at 410 kg S.haˉˡ caused asignificant decrease in fruit decay percentage during the second growing season and an increase in total soluble solids during the first growing season.Decay percentage,percent of weight loss, and total soluble solids and titratable acidity increased with increasing storage period .There was also adecrease in vitamin C contant with increasing the storage period for both growing seasons.The method of storage had a significant effect on studied characteristics,as cold storage caused a significant decrease in percentage fruit decay during both seasons and weight loss during the first growing season ,as compared with storage at room temperature.However,storage at room temperature caused asignificant increase in ascorbic acid content ,and total titratable acidity of the fruit as compared with cold storage and for both growing season .The total soluble content of the fruits increased for there stored at room temperature as compared to cold storage during the first growing season only.

تاثير الرش ببعض مضادات الاجهاد البيئي في بعض الصفات الفسيولوجية والتشريحية والانتاجية لنخيل التمر Phoenix dactylifera L. صنف الحلاوي == Effect of Spraying Some Environmental Stress Compounds on Some Physiological and Anatomical and Productivity Characters of Phoenix dactylifera L. CV. Hillawi

Author name: منتهى عبد الزهرة عاتي سدخان
Supervisor name: عل حسين محمد الطه
General topic: Agricultural sciences
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted in a private orchard at Abu El - Khasseb District, Basrah Governorate during the growing seasons of 2014 and 2015 to investigate the effect of foliar spray of some environmental stress compounds of Fegeamino and Drin at concentrations of (2 and 4 ) ml.L - 1 and Ascorbic Acid at concentrations (500 and 1000)mg.L - 1 on some biochemical , anatomical characters and yield components of date palm cv. Hillawi during the ripening stage of Khalal , Rutab and Tamir. Results showed that Ascorbic Acid treatment at 500 mg.L - 1 recorded significant increases in fruit and flesh and seed fresh weight of the first season, fruit length and diameter of both seasons , moisture content of the first season , dry matter percentage of the second season , potassium to sodium ratio and thickness of inner mesocarp ,whereas Ascorbic Acid treatment at 1000 mg.L - 1 had significant increases in fruit total acidity of the second season , total chlorophyll and carotene pigments concentration of leaf ,proline concentration of leaf , peroxidase activity of leaf , exocarp and outer mesocarp thickness of fruit , bunch weight and total yield of tree at Tamir stage . Results showed also that Drin treatment at 2 ml.L - 1 recorded significant increases in vitamin C concentration of leaf, phosphorus and potassium concentration of leaf , peroxidase activity of fruit , tannin layer thickness, and fruit ripening percentage at Tamir stage , whereas Drin treatment at 4 ml.L - 1 had significant increases in fruit dry matter percentage of the first season , total soluble solids of the second season, free amino acids at Rutab stage , soluble protein of fruit at Khalal stage , total carbohydrates of leaf , nitrogen concentration , ,total chlorophyll concentration of fruit, catalase activity of leaf and fruit, and number of cells in mm2 of inner mesocarp. Fegeameno treatment at 2 ml.l - 1 gave significant increasesin fruit and flesh fresh weight of the second season , fresh weight ,gibberllins concentration, carotene pigment concentration of fruit , cell length and width ,whereas Fegeamino treatment at 4 ml.L - 1 had significant increases in seed weight of the second season , auxins , abscic acid concentration and vitamin C of fruit . Control treatment had significant increases in fruit moisture content of the second season , total soluble solids and total acidity of the first season, soluble protein of leaf and potassium to sodium ratio of leaf . Once and twice and trice sprays of trees with environmental stress compounds gave significant increases in most of the studied characters .Results of the most influencail bio - combination treatments were Fegeamino at 2ml.L - 1 with once spray which gave significant increases in fruit and flesh and seed fresh weight of the second season, fruit length of the first season and cell length ,whereas Fegeamino treatment at 2ml.L - 1 and twice spray gave significant increases in fruit ripening percentage , fruit soluble protine ,leaf potassium to sodium ratio, vitamin C of fruit and cell width , and Fegeamino treatment at 2ml.L - 1 and trice spray had significant increases in fruit dry matter percentage of the first season, carotene pigment concentration, gibbirellins concentration , exocarp thickness and number of cell in mm2 of inner mesocarp.Drin treatment at 4 ml.L - 1 and trice spray increased significantly fruit total acidity, free amino acids , fruit total chlorophyll and carotene pigment concentration and, catalase activity . Ascorbic Acid treatment at 500 mg.L - 1 with twice spray increased significantly fruit and flesh fresh weight of the first season, fruit length and diameter of the second season , and leaf soluble protein , whereas Ascorbic Acid treatment at 1000 mg.L - 1 and trice spray increasedsignificantly bunch weight , total yield, peroxidase activity of leaf and fruit, and catalase activity of leaf. Control treatment of bio - combination gave significant increases in fruit moisture content of the second season and total soluble solids of fruit .Results of protein pattern showed the occurrence of deferential gene expression in which a number of bands appeared on the gel were differed in numbers and location depending on type of treatments .

استخلاص وتوصيف الكولاجين من وتر ارجل الجاموس وجلد سمك المزلق ودراسة بعض صفات الكولاجين المتحلل انزيميا == Extraction And Characterization of Collagen From Buffalo Tendon Legs and Fish Oriental Sole Skin and Study of Some Properties of Enzymatically Hydrolysed Collagen

Author name: محمد زيارة اسكندر
Supervisor name: ام البشر حميد جابر الموسوي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تاثير احلال نسب مختلفة من مجروش نوى التمر محل الشعير مع او بدون اضافة خميرة الخبز (Saccharomyces cerevisiae) في اداء الحملان العرابية الذكرية == Effect of replacing different ratios of crushed Date Stone instead of barley with or without addition of Saccharomyces cerevisiae on performance of Arabi Male Lambs

Author name: كريم حمادي مهنى ابو سلوى
Supervisor name: محارب عبد الحميد طاهر | عـماد فلاح الجاسم
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted in the Animal Farm of the College of Agriculture - University of Basrah. A total of 28 Arabi male lambs were used, with average age of 5 months and mean body weight of 21.74 ± 1.33 Kg. Lambs were distributed equally and randomly into 7 nutrition rations in two experiments. First experiment was a growth experiment which lasted for 90 days. The second was digestion field experiment and lasted for 13 days after the end of the first experiment. The experimental rations were as follows : Ration - 1 - (control) - fed on concentrate ration only by 3% of body weight. Ration - 2 - fed on 90% of the control ration and 10% of ground date stones substituted for the barley.Ration - 3 - lambs fed on the ration 2 with addition of 3 grams of bakery yeast (Saccharomyces cerevisiae) / Kg of feed. Ration - 4 - fed on 80% of the control ration with substitution of 20% of date stones for barley.Ration - 5 - lambs fed on the ration 4 with addition of 3 grams of bakery yeast per Kg of feed.Ration - 6 - fed on 70% of the control ration and 30% of date stones.Ration - 7 - lambs fed on the ration 6 with addition of 3 grams of bakery yeast per Kg of feed.Results were as follows : 1 - Mean final body weight (34.00 Kg) except Control ration, total gain weight (12.75 Kg) except the control and 2 rations and daily gain weight (141.67 g/ day) of lambs in ration 3 were significantly higher than animals in other rations.2 - Animals of ration 3 recorded the highest feed consumption arithmetically (0.829 Kg/ day/ lamb) and the best feed conversion ratio arithmetically too (5.85 Kg dry matter/ Kg body weight gain).3 - Substitution of date stones with addition of bakery yeast affected digestion coefficients of feed nutrients. Ration 3 was superior in digestion coefficients of dry matter, crude protein and nitrogen free extract to other rations. These values were 70.64, 72.11 and 80.64 %.4 - Results showed significant differences between means of pH of rumen fluid and total bacterial count which Significant decreases in each of 6 and 7 rations than other rations while in the counts of cellulolytic digestion bacteria, ration 3 were significantly than animals in other rations but not differ significantly from that of the ration 5 while it was similar with control ration. 5 - No significant differences between rations were noticed in means of red blood corpuscles, white blood cells, hemoglobin, packed cell volume, cholesterol concentration, glucose concentration and concentration of the enzymes GPT and GOT. While differences between means of blood urea concentration and total protein of blood were significant.6 - Mean empty body weight, hot carcass weight, dressing percentage on the basis of live weight and empty body weight of lambs in ration 3 28.02 Kg, 16.18 Kg, 47.59% and 57.74% respectively were significantly higher than that of animals in other rations except control ration in hot carcass weight.7 - The highest weights of rib cut (rib 7 - 12), leg cut and loin cut were for animals in ration 3 (1.61, 5.00 and 2.40 Kg respectively) than other rations but in the loin cut, the ration 3 was similar to the control ration 2.16 Kg.8 - For body measurements, the highest leg circumference were recorded for animals in rations 2 and 3 (35.00 and 36.50 cm respectively) than other rations but these rations were similar to the control ration. While the highest rib eye muscle area was for carcasses of animals in ration 3 (14.41 cm2) but this value did not differ significantly from that of the control animals (13.45 cm2).9 - In the case of some carcass muscle weights, the muscle Supraspinatus weight were higher in the animals of the ration 3 (176.67 g) but it was similar to control ration (164.25 g). Weight of the muscle Biceps femoris was higher in the carcasses of animals in ration 3 (625.75 g).10 - Carcasses of animals in ration 3 recorded the highest dissected lean meat 931.70 g and fat 280.55 g in rib cut, but no significant differences were registered for dissected bone weight among the different rations. Also weights of the internal organs of the animals namely : liver, heart, kidneys, lungs, spleen and the testicles for animals in ration 3 were higher than that of other rations.11 - Mean weights of separated fat of carcasses were higher for animals of the 2 and 3 rations (1.93 and 1.95 Kg respectively) in comparison with animals of other rations but these rations were similar to the control ration 2.08 Kg.12 - Substitution of date stones in percentages of 10% and 20%, with the addition of bakery yeast caused significant improvement in organoleptic characteristics (color, tenderness, juiciness and overall acceptability). Evaluation of samples of rations 3 and 5 were superior to that of other rations.13 - Rations 3 and 5 gave the best results for water holding capacity in meat. No significant differences were obtained in cholesterol concentration and pH of meat.Results showed that the lowest values of cooking loss were in ration 3 (43.30 %).No significant differences were observed in chemical composition of theLongissimus dorsi muscle (moisture, protein, fat and ash) between rations.

تاثير اضافة بعض الاحماض الامينية وعصير البنجر الى المخففات في بعض صفات السائل المنوي المبرد والمجمد للكباش == Effect of adding some amino acid and Beet juice to the dilutors on some cooling and freezing rams semen traits

Author name: فلاح عبد المحسن عبد الله الاسدي
Supervisor name: عماد فلاح حسن | عبد الرزاق نعيم خضير
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted at the agricultural experimental Research Station / College of Agriculture / University of Basrah form 3rd /1/2015 to 3rd /1/2016. A total of 12 mature rams (4 Najdi , 4 Arabi and 4 crosses) aged 3 - 3.5 years with nearly similar weights . Nutrition depended on concentrate ration ( 47 % wheat bran , 50 % barely ,1% urea , 1% CaCo3 and 1% Nacl ) , with 16.58 % crude protein and TDN 72.17% and was given 0.5kg /animal twice aday (at morning and afternoon)500 - 750 g / animal green fodder with wheat straw was given as it was available in the farm , cubic mineral and water provided freely at the day. Rams were training for semen collection for 30 days before the beginning of the study by using artificial vagina used for sheep. Semen was collected three time each month. The study includes two experiments : First experiment : It was designed to study the effect of breed , season ( spring , summer , autumn and winter ) and month on semen characteristics physical , sexual hormones ( testosterone , ICSH and SSH) of blood serum and level of enzymes ( AST , ALT and ALP ) of blood plasma.Second experiment : It included ten diluters from local materials (Beet pulp juice ) with 5 , 10 and 15% egg yolk and amino acids ( Glycin and cysteine ) with concentration of (5, 10 and 15) mmol. It also included the effect of type of dilutor , cooling , freezing storage period ( one and two months) in liquid nitrogen ( - 1960 C) on individual motility and live sperm percent .bThe results can be summarized as follow : 1 - Arabi rams exceeded (P<0.05) Najdi and crosses rams in their semen pH , spring also showed higher (P<0.05)pH followed by winter in comparison with summer and autumn .May showed higher (P<0.05) semen pH in comparison with other months.2 - Najdi rams exceeded (P<0.05)Arabi and their crosses in mean ejaculate volume, mass and individual sperm motility , sperm concentration , live sperm percent and mean testes circumference .3 - Summer and autumn showed higher (P<0.05) in meanejaculate volume , mass and individual sperm motility , sperm concentration , live sperm percent and mean scrotal circumference than winter and spring . Spring also showed higher (P<0.05) dead sperm percent followed by winter in comparison with summer and autumn .4 - August showed higher (P<0.05) ejaculate volume and mass and individual sperm motility followed July and June in comparison with other months. September , August and October exceeded (P<0.05)in comparison with other months sperm percent . October showed higher (P<0.05) in live sperm percent in comparison with other months . Highest (P<0.05) dead sperm percent happened during March followed by April and May . Scrotal circumference was high (P<0.05) during September followed by November and October.5 - Najdi and Arabi rams showed significant (P<0.05)differences in testosterone and ICSH hormones in comparison with their crosses . However , crosses exceeded (P<0.05)Arabi and Najdi rams in mean of SSH hormone. Autumn followed by summer exceeded (P<0.05) wintercand spring in testosterone , ICSH and SSH hormones. September and October showed higher (P<0.05)testosterone, ICSH and SSH hormones in comparison with other months.6 - Arabi rams exceed (P<0.05) both Najdi and their cross by enzymes concentration ( AST, ALT and ALP) of semen plasma . Spring and winter exceeded (P<0.05) summer and autumn by mean of ALT and AST enzymes. However , summer and winter exceeded (P<0.05) autumn and spring by mean of ALP concentration . March showed highest (P<0.05)AST and ALT concentration , while August showed highest (P<0.05) ALP concentration.7 - Amino acid diluter ( 15 mmol glysin and cysteine ) and 10 % beet pulp juice showed highest (P<0.05) individual motility after cooling and freezing for month and two monthsduring all months of year. While the beet pulp juice 15% and control showed the lowest means (P<0.05) of individual motility after cooling and two months of freezing.8 - Glysin 10 and 15 mmol , cysteine 10 mmol and beet juice 10% showed significant (P<0.05) increase in live sperm percent after and cooling and freezing . On other hand beet pulp juice 15% and control recorded the least value (P<0.05) . Beet pulp juice and control showed the highest (P<0.05) level of dead sperm percent after cooling and freezing in contrary with glysin 10 mmol and cysteine 15 mmol

تاثير الاحلال الجزئي او الكلي للحنطة المخمرة محل الذرة الصفراء في بعض الصفات الانتاجية والفسلجية لفروج اللحم == Effect of Replaced Partial or Complete Fermented Wheat Instead of Corn on some Productive and Physiological Traits of Broiler Chicks

Author name: علي جبر حمود علي الحميداوي
Supervisor name: عبد الله عبد المنعم محمد | جعفر محمد جاسم
General topic: Agricultural sciences
Specific topic: Zoology - Nutrition
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study included four experiments, two test laboratory experiments and two field experiments. The first experiment involved the use of laboratory quantities of inoculums dose and different periods of fermentation on the local wheat fermented with Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor to obtain the best percentage of crude protein with less fiber content between different fermentation methods. The seconds experiment depended of the best results of the first experiment and re - fermentation process with the addition of urea by 2% to provide a source of nitrogen for use by microorganisms to protein syntheses. The first field experiment was to see the effect of substitution of fermented wheat with Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor partially or completely replace corn in diets on the productivity of broiler chickens. Digestion experiment was to determine the proportion of digested crude protein and crude fiber in the various diets also conducted. In this experiment 360 unsexed chicks of broiler class (Ross 308) is distributed over eight treatment by 3 replicates per treatment (15 chicks for replicate) as follows. T1 and T2 : diets control with or without antibiotic respectively; T3 and T4 : substitution partial and complete of fermented wheat with Saccharomyces cerevisiae; T5 and T6 : substitution partial and complete of wheat fermented by Iraqi probiotic; T7 and T8 : treatment replace partial and complete rumen liquor fermented wheat. As the second field experiment designed to study the replacement of wheat fermented with Saccharomyces cerevisiae and Iraqi probiotic and rumen liquor with urea partially or completely replace corn in diets on the productive performance of broiler chickens as well as digest it conducted a second test after the end of the second field experiment. In this experiment, A 450 chicks were used and distributed over 10 treatments each with three replicates (15 chicks for replicate)Btreatments are : T1 and T2 partial and complete replacing of wheat without urea instead of corn, respectively (controls); T3 and T4 partial and complete replacing of wheat with urea instead of corn, respectively (controls); T5 and T6 partial and complete replacing of wheat fermented by Saccharomyces cerevisiae with urea instead of corn, respectively; T7 and T8 partial and complete replacing of wheat fermented by Iraqi probiotic with urea instead of corn, respectively; T9 and T10 partial and complete replacing of wheat fermented by rumen liquor with urea instead of corn, respectively. The current studies included the following results : The first laboratory experiment : Fermented wheat by Saccharomyces cerevisiae with the amount of inoculums get 2 gm. - 1 Kg of wheat and 14 days fermentation period the archieved highest percentage in crude protein, followed by treatment of the fermented wheat rumen liquor with the amount of inoculums dose of 4 ml. Kg - 1 wheat and fermentation period of 14 days and then the treatment of Iraqi probiotic fermented wheat 5 gm. Kg - 1 and fermentation period of 21 days to access to 14.64% ,14.43% and 13.59%, respectively, in the treatment of non - fermented wheat recorded of 11.59% crude protein.The second laboratory experiment : The seconds experiment depended three of the best results of the first experiment (Fermented wheat by Saccharomyces cerevisiae with the amount of inoculums get 2 gm. - 1 Kg of wheat and 14 days fermentation period, fermented wheat by rumen liquor with inoculums dose of 4 ml. Kg - 1 wheat and fermentation period of 14 days and fermented wheat by Iraqi probiotic 5 gm. Kg - 1 and fermentation period of 21 days) re - fermentation process with the addition of urea by 2%.CThe first field experience : fermented wheat treatments by Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor increased significantly (P≤0.05) the live body weight, total weight gain, accumulative feed intake, economic figure, percentage of bone ash, villi height, ratio of villi height to crypt depth of the intestine, number of red blood cells, percentage of hemoglobin as well as the level of calcium and cholesterol in serum recorded significant decrease in favor of fermentation treatments were favorable results for the benefit of the treatment of substitution of wheat fermented by rumen liquor (T8) significantly (P≤0.05) was showen in protein and fibers digestibility in T7 and T8. While no significant defferances recorded in feed conversion ratio, viability, dressing percentage, organs weight, sensory characteristics, intestinal length and crypt depth were not different among all including the control.The second field experiment : The results showed that there were significant increases in final live body weight, total weight gain of T5, and accumulative feed intake for the T6 group, while T10 recorded the best conversion ratio, also T9 and T10 recorded the best economic figure, dressing percentage, percent of hemoglobin and packed cell volume. and Femur bone. As well as best protein and fibers digestibility were recorded as compared with control. Six treatment was showed the best results in blood serum ELISA titer against for Newcastle disease, infection bursa disease, Avian influenza and Bronchitis while non significant difference showed in viability, gizzard and proventiculus percent

التشكل الوراثي لجيني اللاكتوفرين والبرولاكتين وعلاقته بانتاج الحليب ومكوناته واداء الحملان قبل الفطام في النعاج العرابية == Relationship between Lactoferrin and Prolactin Genes Polymorphism and Milk Yield and its Components and Pre - weaning Lambs Performance of Arabi Ewes

Author name: علي جاسم عبد الرضا
Supervisor name: اسعد يحيى عايد | سمير اسطيفان حنا
General topic: Agricultural sciences
Specific topic: Zoology - Education
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Polymorphism analysis of lactoferrin and prolactin genes and its relationship with milk yield and its components and birth, weaning weight and growth rate before weaning were studied. DNA was extracted from 77 blood samples of Arabi ewes (21 ewes from Animal Farm, College of Agriculture, University of Basrah and 56 ewes from Animal Farm, College of Agriculture, University of Al - Muthana). Lactoferrin genotypes were determined by using Hind III restriction enzyme, those of prolactin by Hae III enzyme at Genetic Engineering Laboratory/ College of Agriculture/ University of Basrah and College of Animal Resources/ Mullah Thani University/ Islamic Republic of Iran. Milk yield was measured once a week from 52 ewes belong to Animal Farm/ College of Agriculture/ University of Al - Muthana. Samples were chemically analyzed. Birth and weaning weight were recorded as well as growth rate before weaning during a period of 1/2/2013 to 31/12/2013. Results revealed the following : 1 - The possibility of amplifying both genes and get two alleles for each and three genotypes of the lactoferrin gene (AA and AB and BB) and two genotypes (AA and AB) of the prolactin gene.2 - Prolactin and lactoferrin A allele frequencies were 0.94 and 0.66 respectively. AA genotypes frequency was 0.88 and 0.52; AB 0.12 and 0.29 and BB 0.00 and 0.19 of prolactin and lactoferrin genes respectively. population was at equilibrium for prolactin gene, but was not in lactoferrin gene.3 - Heterozygosity levels were 11.69% and 28.57% for prolactin and lactoferrin genes respectively. inbreeding coefficients (Fis) were - 0.05 and 0.345 for both genes respectively. University of Basrah and Al - Muthana herds showed very high genetic correlation (0.999).4 - The overall means of total and daily milk yields and lactation length were 61.13±2.76 kg, 0.582±0.04 kg and 104.75±1.95 days respectively. Ewe age and lamb sex did not significantly influence the above traits. However, ewes lambed during February exceeded thoselambed in September by daily milk yield (0.661 vs 0.577 kg) and lactation period (110.00 vs 100.00 days).5 - The overall means of total fat, protein and SNF and their percentages were 2.65 Kg and 4.33%, 2.5kg and 4.11% and 11.98% respectively. Only SNF was significantly influenced by ewes age and lamb age.6 - The overall means of birth and weaning weights and pre - weaning daily growth rate were 4.48±0.39 kg, 21.21±0.89 kg and 161.71±8.47 gm./day respectively. Lambs birth weight was significantly influenced by ewes age, ewes aged 4 and 5 years got heavier lambs than those aged 1 and 3 years.7 - Lactoferrin genotypes significantly affected protein and total solids not fat, as AA genotype exceeded the other genotypes. Fat of AA, AB and BB genotypes were 2.85, 2.23 and 2.63 kg respectively. while values of SNF were 7.24, 5.78 and 7.51 respectively.8 - There was arithmetic increase (not significant) in all traits for the AB genotype over AA genotype of prolactin gene.9 - Allele B of prolactin gene positively and significantly influenced most trait. It shared about 75% of each trait variation. That of lactoferrin gene shared about 25% only.10 - Heritability of SNF% and quantity was high (0.652 and 0.666 respectively). Those of birth weight and growth rate were medium (0.309 and 0.346). All other traits had low heritability.11 - Highest value of BLUP was recorded for daily milk yield, total milk yield and adjusted milk yield for 150 days (0.032, 3.072 and 2.220 kg respectively). The lowest values were - 0.021, - 2.071 and 0.00 kg respectively. Their ranges were 0.053, 5.143 and 2.220 kg respectively.12 - The lowest value of BLUP of fat%, protein% and SNF% were - 1.724, - 2.448 and - 4.565% respectively. the highest values were 2.058, 2.466 and 3.764% respectively with a range of 3.782, 4.914 and 8.329% respectively.13 - The lowest values of BLUP of birth and weaning weight and growth rate were 3.00 kg, 19.9 kg and - 1.20 g/day, the highest were 5.5 kg, 23.45 kg and 1.03 g/day with ranges 2.5 kg, 3.86 kg and 2.23 g/day respectively.14 - Breeding values of prolactin AB genotype of all studied traits exceeded those of AA genotype. However, those of lactoferrin AB and BB genotypes exceeded those of AA genotypes.

التعبير الجيني ووفرة نسخ mRNA لجينات نقل العناصر الغذائية في الامعاء الدقيقة لفروج اللحم باختلاف مستوى الطاقة والبروتين في العليقة == Gene Expression and mRNA Abundance of Nutrients Transporter Genes in The Small Intestine of Broiler of Different Levels of Protein and Energy in Diets

Author name: علي احمد عبد الكریم ابراهیم العامري
Supervisor name: اسعد یحیى عاید | عبد لله عبد المنعم
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted in both Poultry Farm/Animal Resources Department/ College of Agriculture / University of Basra and laboratories of Molecular Biology/ London/ United Kingdom (The Molecular Oncology Unit Laboratories of Guy's and St Thomas's Hospital/ Kings College London/ UK). Molecular analysis of the samples and the detection of a number of gene expression of several feed transporter genes in the small intestine of broiler chickens when fed diets with different levels of energy and protein. A total of 225 unsexed one day old broiler chicks (Ross breed) were randomly distributed on five experimental treatments each with three replicates of 15 chicks. Feeding was ad libitum during the whole period of experiment that lasted 35 days.The first treatment was control with 22.3 and 20 % crude protein and 2910 and 3001 kcal/kg for starter and growth period respectively. The second treatment consisted 24.1 and 22 % crude protein and 2920 and 2978 kcal/kg for starter and growth period respectively. The third treatment consisted 20 and 18 % and 2898 and 2996 kcal/ kg for starter and growth period respectively. The fourth treatment consisted 21.9 and 20 % and 3107 and 3199 kcal/ kg for starter and growth period respectively. The fifth treatment consisted 22.2 and 20 % and 2720 and 2800 kcal/ kg for starter and growth period respectively. The results revealed the followings : - The study showed the ability to amplify all genes and find their abundance according to their primers. - The highest value of APN gene abundance of female in the duodenum (1.86 ng) fed diet with 22 % cp and 3199 kcal/kg ME. - Highest abundance (6.19 ng) of Pept1 mRNA showed by males in their jejunum, when fed a diet consisted 22 % cp and 3199 kcal/kg ME. - The greatest value of b - AT and CAT2 amino acids transporter genes effect was revealed by males fed diet with 22 % cp and 3001 kcal/kg ME. - Females fed 20 % cp and 3199 kcal/kg ME diets reordered highest SGLT1 gene expression (4.70ng) within their ileum. - Males fed 20 % cp and 3199 kcal/kg ME diets reordered highest GLUT5 gene expression (6.37 ng) within their ileum. - All genes associated positively and significantly with body weight, growth rate, carcass cuts percentages (legs, breast, intestine length and weight) at the age of 35 days and negatively with feed consumption and feed conversion ratio. - Amino acid and peptides transporter genes abundance improved with an increase in diet protein, that of saccharide transporter improves with the increase of ME level in the diets. - Male gave higher mRNA copies of most genes in comparison with females, except that of SGLT1 and NPA which increased in females. - It is possible to manipulate nutrient transporter gene expression by feeding. That may reflect on birds performance.

استخلاص مضادات الاكسدة بمساعدة الموجات الدقيقة من اليرون حبة القمح وجنينها ونخالتها ودراسة تاثيرها في الصفات الريولوجية للعجين == Extraction of Antioxidant with Assisted Microwave from Aleuron Wheat Grain, Germ and Bran and Study its Impact in the Rheological Properties of Dough

Author name: علاء محمد صالح مجيد المسافر
Supervisor name: علي احمد ساهي | علي خضير جابر الركابي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to investigate and test the activity of three essential parts of wheat seed Aleurone, germ and bran which were used as secondary products from grinding grain to feed animals. diversity solvents (distilled water, chloroform, hexane, ethanol, methanol) were applied to extract the bioactive compounds by both traditional and modern extractions.The total phenolic compounds, antioxidant activity, reducing power, metal chelating, and scavenging of hydrogen peroxidase were determined for all three essential parts of wheat. The stability of the extracts was studied toward temperature and pH at the interval time. In addition, the synergistic effect of the extracts was studied by mixing with different concentrations of ascorbic acid and alpha tocopherol. The extracted compounds were identified using gas chromatography mass spectroscopy (GC - Mass).The ethanol extraction of wheat germ had the highest antioxidant activity, so it was chosen to prevent oxidation in the corn oil. Moreover, the ethanol extractions for germ, bran and Aleurone were added to wheat flour (80 % extraction) in order to study their effects as improvers in the rheological properties of wheat flour dough. The results were as follows : 1 - The microwave - assisted extraction for 40 sec was able to extract the highest level of phenolic compounds from wheat germ compared toconventional extraction conditions. The total phenolic compounds were 556.6 μg gallic/ml, 426 μg gallic/ml for ethanol and methanol extractions, respectively.2 - The microwave - assisted extraction was able to give the highest antioxidant activity (53%) at concentration of 100 mg/ml from ethanolic wheat germ extraction compared to synthetic antioxidant BHT ( 92 %). In addition, the wheat germ had the highest reducing power (163.88 %) at concentration of 5 mg/ml , while the percentage of reducing power for both ethanolic extraction of Aleurone and ethanolic extraction of wheat bran were 112.63 % and 79.68 %, respectively. In contrasts, all of above extractions had lowest reducing power compared to BHT and alpha tocopherol 249.8 % and 223.5 % respectively at the same concentration. Moreover, the wheat Grem had the highest percentage for both Ferrous chelating and scavenging of hydrogen peroxidase.3 - Ethanolic extracts of Aleurone, bran and germ have a higher antioxidant activity in natural pH while decreased when treated with acid and alkaline. In addition, the ethanolic wheat germ was able to give the highest antioxidant activity at 100°C for 100 min wich read 62.2% compared to others and gave synergistic effect for bothascorbic acid and alpha tocopherol exhibited 91.90 % of antioxidant activity at concentration 60 mg/ml.4 - Identification of ethanolic and methanolic extract using GC/MS showed presence of bioactive compounds such as ascorbic acid, benzoic acid, phytosterol compounds, Isoeugenol and alkylresorcinols5 - Ethanolic extracts of germ with concentration 0.05, 0.10, 0.15, 0.20, 0.25 mg/g was added to corn oil in order to compared with BHT 0.05% and stored at 45°C for 28 days, the result showed that the ethanolic wheat germ at 0.25 mg/g had the ability to decrease the hydrogen peroxide for the corn oil 6.8 meq/kg at end of stored time compared BHT (0.05 %) 4.3 meq/kg at the same conditions.6 - The ethanolic Aleurone extraction possessed a positive effect on the rheological properties when it was added as improvers to the wheat flour ( 80 % extraction). The dough consistency was 6.7 min, which considered highest reading among all treatments. In addition, the recorded elastic index was 3.2, 2, and 1.8 at interval times 45, 90, 135 min, respectively.

تاثير اضافة الخليط التازري لمسحوق حليب الكيفير المجفف والمصنع في بعض الصفات الانتاجية والفسلجية والمناعية لفروج اللحم == Effect of Addition of Synbiotic of Dried and Processed Kefir Milk Powder in Some of The Productive, Physiological and Immunological Characteristics of Broiler Chicken

Author name: عقيل طوينة عودة
Supervisor name: عبد الله عبد المنعم محمد
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Four experiments were carried out for this study, two field and two laboratory, to manufacture a Synbiotic for milk powder Kefir ( MPK ) and loaded with some feed material with or without sugar beet milled or Helianthus tuberosus powder and the effect of adding it to some of the production , physiological and immunological characteristics of the chickens . Where the first laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agricultur - University of Basrah , for the period from 10 / 4 / 2015 to 7 / 5 / 2015 for the purpose of manufacturing a Synbiotic of MPK.The second field experiment was conducted in a commercial field for poultry in Al - Zubair / Basrah during the period from 24 / 1 / 2016 to 27 / 2 / 2016 , which included selecting the best biomass of MPK loaded on wheat , corn , soybeans and wheat bran with the addition of Helianthus tuberosus powder , So that one gram of MPK and loaded on the previous materials can bacteria . In this L. acidophiluscfu / ml 8least 10be provided at experiment, 450 unsexed one day of the Ross 308 chicks, at a rate of 40 g / chick were distributed randomly to six Treatments , three replicates per treatment , 25 birds per replicate and the treatments were as follows ; T1 : Control treatment included a standard diet . T2 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach . T3 : A standard meal with 3 g of MPK loaded on wheat/ kg feed .T4 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T5 : A standard meal with 3 g of MPK loaded on soybeans / kg feed. T6 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed .The third field experiment was conducted to determine the best effect of the synbiotic MPK loaded with wheat, corn, soybeans and wheat bran and compared with three foreign probiotics in the productive, physiological and immunological characteristics of chicks. This experiment was conductedbduring the period from 4 / 10 / 2016 until 9 / 11 / 2016, using 600 unsexed chickens with one day of the Ross 308 at a rate of weight of 40 g / chick .The chicks were randomly distributed into eight treatments with three replicates per treatment and 25 birds per replicate. The experiment was designed as follows : T1 : Control treatment included a standard diet, T2 : A standard meal with 3 g of MPK loaded on wheat/ kg feed . T3 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T4 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed. T5 : added treatment 1/2 g of probiotic Labzyme / kg feed , Korean - made , T6 : added treatment 1/2 g probiotic Biozyme / kg feed , German - made , T7 : added treatment 1/2 g probiotic Biolac / kg feed , Vietnamese made , T8 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach .The fourth laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agriculture, University of Basrah for the period from 6 / 3 / 2016 to 7 / 4 / 2016 for the study of antibacterial activity in the kefir milk and compare it with Biozyme , Pencilin, Gentamycin, Tetracyclin and Neomycin against Staphylococcus aureus and Escherichia coli causing bacterial necrosis with Bacterial Chondronecrosis with Osteomyelitis ( BCO ) . The results of the study included the following : First laboratory experiment ;The results of the first experiment showed that the wet biomass rate of fermented kefir milk was 70 % . This high percentage is evidence of the growth of Lactate Bacteria in this product. The results showed that wheat bran and wheat bran and corn were the best loaded significant materials (p <0.05) Compared to soybeans in terms of growth and reproduction of lactic acid bacteria. Adding Helianthus tuberosus powder increased the number of bacteria in dried kefir milk compared sugar beet milled , which reduced the number of bacteria in MPK .Second field experiment : cThe results of the experiment showed the following : 1 - There were significant increase (p <0.05)) in the final body weight rate and the body weight gain and Food conversion efficiency, as well as a significant decrease (p <0.05) In the amount of feed consumed for the sixth, third and fourth treatments, supplemented with synbiotic MPK loaded on wheat bran, wheat and corn compared with other treatments .2 - Treatments sixth , third , fourth had significant (p<0.05) decrease mortality percent as comparing with control and treatment two .3 - The sixth , third , and fourth treatments were significantly higher (P <0.05) in the production index and the performance index and drcrease value of economic efficiency compared with other treatments .4 - Total protein and globulin in plasma serum were increased significant (p<0.05) and decreased (p <0.05) in the concentration of triglycerides and cholesterol in the sixth, third and fourth treatments supplemented with synbiotic MPK , loaded on wheat bran , wheat and corn , as well as the increased of the third, sixth and fourth treatments significantly (p <0.05) in the concentration of ALP enzyme compared to other treatments .5 - The results showed that the fifth, sixth, third, and fourth treatments did not differ significantly in antibodies titer against Newcastle ( NDV) and the Comboro ( IBDV ) disease, but significantly exceeded (p <0.05) compared to the first and second treatments .Third field experience : According to the results of the second field experiment, the best three treatments were added to the synbiotic MPK and loaded on wheat bran , wheat and corn . It was compared with three foreign prpbiotics , the treatment of addition of amoxicillin antibiotic and control treatment. The results of the experiment showed the following ;d1 - The second , fourth , third and sixth treatments, supplemented with probiotic, MPK , loaded with wheat , wheat bran , corn, and labzyme were significantly higher (p <0.05) in body weight and weight gain . As well as a significant decrease (p <0.05) in the amount of feed consumed and a significant improvement (p <0.05) in the efficiency of food conversion compared to other experimental treatments .2 - The first, fifth, and seventh treatments were significantly higher (p <0.05) in the mortality percent as comparing , to other experimental treatments .3 - A significant (p <0.05) increased was observed in the production index and the performance index and significantly decrease in the economic efficiency value for the second, fourth, third and sixth treatments.4 - The second, fourth, third and sixth treatments were significantly higher (p <0.05) in dressing percentage , tibia and thoracic characteristics , as well as a significant increase (p <0.05) in the weight of the cecum compared to other treatments.5 - There was a significant increase (p <0.05) in the weight of Bursa fabricius for the first, eighth and seventh treatments, as well as a H/L ratio was significant (p<0.05) decrease in the second, third, fourth and sixth compared to other treatments.6 - Total protein and globulin in plasma serum were significant (p<0.05) increased and decrease significantly(p <0.05) in the concentration of triglyceride and cholesterol in treatments second, fourth, sixth and third , compared to other treatments.7 - The superiority of the second, third, and fourth treatment was significantly higher (p <0.05) in the concentration of ALP enzyme in plasma serum compared to other treatments.8 - The third, seventh, second, fourth, and sixth treatments recorded the highest significant mean (p <0.05) of the degrees granted to them by theeEvaluator in the flavor, tenderness and juicy compared to the fifth, first and eighth treatments .9 - The second, seventh, third, fourth, and sixth treatments were significantly higher (p <0.05) in lactic acid in intestinal fluid , as well as There were significant (p<0.05) increase in digestibility of dry matter , protein and fat in treatments fourth, second, third and sixth as compared with other .10 - The fourth, second, third, and sixth factors significantly exceeded (p <0.05) in weight and length of the tibia , bone index , ash ratio , and skeletal weight of the chickens, as well as The second, fourth, third, and sixth treatments were significantly higher (p <0.05) in the level of calcium and phosphorus in the bone compared with other .11 - The first, eighth, fifth and seventh treatments were significantly higher (p <0.05) in the rate of food passage in the gastrointestinal tract compared with other.12 - Significant increase (p<0.05) in villi length and crups depth in treatments fourth, second and third as compared with seventh, fifth, eighth and first.Fourth laboratory experiment : The results of the experiment showed no significant differences in the treatment of MPK with gentamycin in diameter (mm) inhibition of Staphylococcus aureus, Escherichia coli, Which cause BCO disease , while significantly increase (P <0.05) with Pencilin, Labzyme, Tetracyclin , Biozyme in diameter of the inhibition zone to the staphylococcus aureus and the diameter of the inhibition zone of Escherichia coli.

تاثير فاصلة ومعاملات الري وتغطية سطح التربة في بعض خصائصها وانتاج نخلة التمر Phoenix dactylifera L. == Effect of interval and methods irrigation in addition soil surface mulching on some soil properties and production of date palm Phoenix dactylifera L

Author name: عبد الرحمن داود صالح الحمد
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in an agricultural field which located at Abu - AlKhaseeb distruct /20 Km south of Basra City , during the season of growth (2013 and 2014) on a land area about one hectare, a clay soil texture and classified as Typic torrifluvents. In order to study the effect of method and interval irrigation and mulching soil surface in some physical and chemical properties of the soil and production of date palm Phoenix dactylifera L. Hillawi c.v. The irrigation method factor included four treatments; drip irrigation method (D) and surface irrigation (S) and rotation method (DS) (rotation drip irrigation with surface irrigation in binary cycle) and tide irrigation method (T), while the mulching factor included three treatments : nylon (N) and coverage waste (W) and no mulching (no). Irrigation interval factor included three treatments are (4 ,8, 12) day. Experiment carried out in factorial Experiment with random complete block design (RCBD) with three replications, the addition of irrigation water calculated from the basin American evaporation (Evap.Pan Class - A - ), which was erected in the study site, plus 20% as leaching requirements.Below is a summary of the study results : 1 - The addition of irrigation water by the irrigation methods (D, DS, S) increase the moisture content significantly for different depths of soil compared with traditional irrigation treatment (tidal). Surface irrigation treatment shows higher values in the moisture content, followed by treatment of irrigation alternately then treated drip irrigation, irrigation with treated showed tidal lowest values. The irrigation methods (drip, drip - surface, surface) increased in moisture content values with lack of a period irrigation, especially when the interval 4 day. Moisture content of the irrigation methods treatments increased significantly values morally using coverage, particularly nylon except irrigation treatment tidal which were not increase significantly. The moisture content values increase significantly with depth for all experimental treatments, and she took to decline at the end of the first season and the second compared to the first season.2 - The treatment of traditional irrigation (tidal) showed the lowest values in the bulk density, followed by treatment of drip irrigation and treatment of rotation and then surface irrigation treatment for all depths .The results showed increased bulk density values of all treatments with depth ,also that the use of the nearby interval and coverage nylon contributed to maintaining the values of bulk density and showed superior in giving the lowest value for the density. Increased bulk density values with the progress of seasonal growth of all treatments and three depths of soil.3 - The use of irrigation methods (drip, drip - surface, surface) to a significant decrease in the values of Mean Weighted Diameter (MWD) compared with the traditional method of irrigation (tidal) and the order of the effect for the irrigation methods is S <DS <D <T. The results showed that values increase with the progress of the growing season, except for the treatment of traditional irrigation (T) with superiority of depth (0 - 30) cm on other depths.4 - The cumulative infiltration and infiltration rate Increased in the soil with the traditional irrigation treatment compared to using other methods of irrigation and the methods irrigation taken following order in the cumulative infiltration and infiltration rateS <DS <D <T. The results showed that the cumulative infiltration and infiltration rate values increased with using mulching especially with nylon and follow short - rang irrigation interval .5 - The use of irrigation methods (drip, drip - surface, surface) decreased the electrical conductivity values on different depths of soil compared with traditional irrigation treatment (tidal), which maintained a significant increase in the electrical conductivity values, especially in (0 - 30 ,30 - 60 ,) cm depth, followed by treatment of drip irrigation and surface irrigation, The rotation treatment DS showed high efficiency in salts washing rate in all depths and recorded the lowest values in the electrical conductivity .The results showed that the using of mulching, especially nylon contributed significantly in reducing the electrical conductivity values, and that the exposed treatment showed higher values, as the results showed that the using of long interval increase in the electrical conductivity values .Other than increase the electrical conductivity values of the surface depth of all treatments and it decreased significantly at both ends of the first and second seasons, compared with the beginning of the experiment except for the treatment of tidal which remained conservative values until the end of the experiment .6 - The use of irrigation methods (drip, rotation, surface) contributed to the significant increase of the values of productivity (weight, size, length, total sugars, dry weight and total yield) of Date Palm compared with the use of traditional irrigation method (tidal ) .The irrigation methods takes the following order in productivity : T <S <D <DS. The results showed that a significant increase in productivity with use short interval and the use of nylon mulching style. The results showed that increase in productivity in the second season compared to the first season.

تاثير رش التربتوفان والكلايسين واضافة المحفز الحيوي Biohealth للتربة في نمو وحاصل بعض اصناف البطاطا SolanumtuberosumL في الاراضي الصحراوية == EFFECT OF SPRAYING TRYPTOPHAN, GLYCINE AND SOIL APPLIED BIOHEALTH ON GROWTH AND YIELD OF SOME POTATO (SolanumtuberosumL.)CULTIVARS IN DESERT LANDS

Author name: عباس كاظم عبید عباس
Supervisor name: عصام حسین علي الدوغجي | نوال مهدي حمود
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Two experimentswere conducted during autum seasonsof 2013 - 2014 and 2014 - 2015 in Tomato Development Project belong to Basrah Agricultural Director, Khor Al - Zubairin southern Iraq to study the effect of foliar application of tryptophan, glycine and soil appliedBiohealthongrowth and yield of some potato cultivars. The aim of the experiments was focused on the effect of three potato cultivars (Draga, Arnova and Provento) in the first season and (Arizona, Arnova and Rivira) in the second season.Spraying with two concentrations( 100 and 200 mg.l - 1)for both Tryptophan and glycine and 0 mg.l - 1(sprying with distilled water).Spryingwas done twice 45 and 60 days after after propagating.Besides, additiontwo concentrations ofBiohealthinto the soil( 0 and 1 g.l - 1for the first seasonand 0 , 1 and 2 g.l - 1 for the second season). The addition ofBiohealthwas done twice 45 and 60 days after propagatingfor both seasons. The experiments was adopted as factional experiment.Split - Split Plot Design was used with three replication,Least Significant Differences Test(LSD) was used at probability of 0.05. The results could be summarized as follows : 1. Emergence of field parameters Results showed that"Arnova" cultivargave asignificant increases in speedemergence field after 20.51 and 19.43 daysin both season,respectively."Brovento"in first season and “Arezona” in the second seasongave asignificant increasesin the percent of field emergence 96.42 and 89.33%respectively, .2.Vegetative growth parameters Resultsshowed that"Draga"cultivar in the first season and "Rivira" inthe second seasonincreased significantly in mostvegetative growth parameters.Spryingwith 200mg.l - 1 tryptophan in bothseasons gave increased significantly in mostvegetative growth parameters.Application withbiohealth1 g.l - 1 in the first seasonand 1, 2 g.l - 1In the second season gave significant increases in mostvegetative growth parameters.The interactions between the studied factors caused significant increased effects in all vegetative growth parameters on both seasons. 3. Yields parameters Results in the first season showed that "Draga" cultivar gave significant increases in tuber number.plant - 1,total plant yield, marketable yield,total yield, small and large tubers yield (6.10 tuber,379.9g,3.36 ton.donum - 1, 3.71 ton.donum - 1,0.35ton.donum - 1 and2.52 ton.donum - 1,respectively), While "Rivira cultivar in the second season gave significantincreases in tuber weigh,total plant yield,marketable yield,total yieldand large tubers yield(88.38 g, 492.6g,4.50 ton.donum - 1,4.82 ton.donum - 1, 3.87 ton.donum - 1,respectively).Sprying with 200 mg.l - 1 tryptophan in the two seasons gavesignificant increases in tuber weigh (69.08, 74.95 g), total plant yield (389.1 , 438.4 g), marketable yield (3.54, 3.87ton.donum - 1),total yield (3.80, 4.28 ton.donum - 1) and large tubers yield(2.44, 3.08 ton. donum - 1) respectively, While the 100 mg.l - 1 tryptophan increases in tuber number.plant - 1 in the first season(5.76 tuber) and 200 mg.l - 1 tryptophan increases in the first seasonin yield medium tubers (1.10 ton.donum - 1)and in the second season increases in tuber number.plant - 1(5.90 tuber).Application with1g.l - 1 biohealthin the first season gave significant increases in tuber number.plant - 1,total plant yield,marketable yield,total yield,medium and large tubers yield(5.72 tuber, 366.6 g, 3.28 ton.donum - 1,3.59 ton.donum - 1,1.03 ton.donum - 1and 2.26 ton. donum - 1,respectively), while in the second season application with 2 g.l - 1 biohealthgave significant increases in tuber number.plantC 1,total plant yield,marketable yield,total yield,and large tubers yield(5.85 tuber, 423.4 g), 3.75ton.donum - 1 ,4.15 ton.donum - 1 and 3.00 ton.donum - 1,respectively). The interactions between the studied factors caused significant increased in allyields parameters on both seasons.4. Chemical characteristics 4 - 1. Leaves chemical characteristics Spraying with 100 and 200 mg.l - 1 tryptophan in Carbohydrate contents. In the second season200 mg.l - 1glycinegave significant increases in carbohydrate contents.Application with 1 g.l - 1biohealthin second season gave a significant increase incarbohydrate contents.The interactions between the studied factors caused significant increased effects in all leaves chemicalparameters on both seasons.4 - 2. Tubers chemical characteristics Results showed that "Brovento" cultivar in the first season gave significant increases in the percentage of dry matter, starch and specific gravity, "Draga" increases in thepercentage of protein, vitamin C and total solubilesoilds, "Arnova" increases intotal free amino acids. In the second season "Rivira" cultivar gave significant increases inthe percentage of dry matter, starch, specific gravity and total free amino acids and"Arezon" increases in thepercentage of vitamin C and total solubilesoilds.Foliar application with tryptophan at 200 mg.l - 1 in the first season caused an increase in the percentage of dry matter, starch,specific gravity and protein, while 200 mg.l - 1 glycine increases in vitamin C and total solubilesoilds, glycine at100 mg.l - 1increases in total free amino acids.Foliar application with tryptophan and glycine at 200 mg.l - 1in second season caused an increase in the percentage of dry matter, starch andspecific gravity, while 200 mg.l - 1 glycine caused increases in the percentage of protein and total free amino acids, glycine at100 mg.l - 1 increasesin vitamin C and tryptophan at 200 mg.l - 1 in total solubilesoilds.Application with biohealth gave asignificant increase in mosttubers chemical characteristics for both season.The interactions between the studied factors caused significant increased effects in all tuber chemicalparameters on both seasons.

استخلاص وتنقية وتوصيف متحللات بروتينية من مخلفات الاسماك والروبيان واختبار كفاءتها في حفظ اقراص مفروم اللحم البقري == Extraction, purification and Characterization of Protein hydrolysates From Fish and Shrimp by - Products and Assay Efficiency in Beef Patties Storage

Author name: عالية زيارة هاشم الحلفي
Supervisor name: ام البشر حميد جابر الموسوي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was interested in the preparation of bioactive peptides by using shrimp and fish by products.Three types of proteolytic enzymes , Alcalase and Pepsin and Flavourzyme were used for this purpose.The constituents (moisture , protein , fat and ash) of defatted raw and dried materials were studed .The proteolysis action of the three enzymes was observed for 5 hours the peptides chain length of the protein hydrolysates were determined and tested for their antioxidat and antibacterial 240 minutes of enzyme assay was reliance according to the highly antioxidantive and antibacterial properties of produced peptides.The proteolysis of shrimp by - product by Alcalase and Pepsin gave highly antioxidantive activity. The isolation , purification and the peptides bioactivity determination was achieved as below : 1 - The peptides of the both protein hydrolysates were isolated by using Ultrafiltration membranes of 5 MWCO KDa. The isolated peptides were examined for their antioxidative activity. It shown , that the peptides wjich was synthesized by the proteolytic activity of Alcalase has ahigher antioxidative action (53.67%) comparing with pepsin peptides (41.19%) at concentration of 100 mg/mL. The results showed that peptides of hydrolysate enzyme Pepsin give inhibitor zoon against bacteria test in inhibition zoon ranged from 10 - 11 mm and determined peptides content of amino acids and showed glysin , threonine ,valine and lysine, which amounted to 9.11% and 8.94% and 7.51% and 9.16%, respectively in peptides of enzyme Alcalase while recorded amino acids serine 9.69% ,theronine 8.76% ,cystin 14.10% and 7.06% lysine ratios highest peptides hydrolysate enzyme Pepsin respectively.Summaryb2 - purification of peptides by gel filtration was showed four peaks of peptides hydrolysate enzyme Alcalase and three peaks for peptides hydrolysate Pepsin enzyme and tested the antioxidation activity of all peaks and inhibitory to bacteria, which recorded that second peak of the Alcalase enzyme antioxidant activity amounted to 63.28% and peptides The first peak of the same enzyme%48.57 the peptides first and the second peak for hydrolysate Pepsin enzyme was% 41.65 and 55.21% also tested the inhibitory effect against some types of bacteria have been to peptides second peak of the Pepsin enzyme hydrolysate towards the bacteria Escherichia coli, Salmonella typhi, Staphylococcus aureus, Bacillus cereus , Pseudomonas aeruginosa inhibition zoon ranged 13 - 9 mm, and determined the minimum inhibitory concentrations (MIC) of these peptides and found that the concentration of 125 and 250 mg / ml values have affected all types of bacteria testing. The cellular toxicity of peptide peakes in analysis of human red blood cells, and has not any toxic effect observed for all concentrations and different periods incubation of peptides peasks.3 - The molecular weight of peptides two of peak enzymes was determined by electrophoresis and cleared two bandes each peak represents two chain peptide molecular weights 3.71 and 4.37 KDa the first peak and the second peak 3.71 and 4.27 KDa of the hydrolysate Alcalase enzyme and chains peptides first peak of hydrolysate Pepsin enzyme 3.71 and 4.16 KDa the second peak, 3.71 and 3.98 KDa, respectively , The study included determined of amino acids and found that it contains all the amino acid varying percentages depending on peptides peakes hydrolysate protein.4 - The stability of antioxidant peptides towards thermal treatment to different degrees thermal ranged between 25 - 100C and change in pH 2 - 11 and treatment of sodium chloride salt ranged between %2 - 8 was studed and found that the antioxidant activity of peptides and reducing power stable at 60C The ability to binding ion ferrous ,hydrogen peroxide , hydroxyl radical scavenging and superoxide anion scavenging they appear stable at thermal 40C and whenctesting the stability of peptides to change the pH was observed that the peptides two peakes hydrolysate enzyme Alcalase stable at pH 8 but decreases when moving away from this value towards the basic or acidic while shwoed peptides two peakes hydrolysate enzyme Pepsin stability at pH 7, and this stability declined when number pH to 11, and when the treatment with salt sodium chloride showed peptides first peak and second enzyme Alcalase and Pepsin stable antioxidant activity at 4% salt concentration for all tests except the oxidation stability of peptides in binding ferrous ion has showed at 6% salt concentration.5 - when the follow of peroxide values for beef patties treated with peptides two peakes enzymes, The results showed there is adecrease in peroxide values for beef patties treated with peptides of the Alcalase enzyme was more cleared compared to the peptides of first peak for the same enzyme and peptides two peakes Pepsin enzyme also got a decrease in the total number of bacteria and total coliform bacteria and psychrophilic bacteria When the treatment second peptides peak of the Pepsin enzyme concentration of 50 and 100 mg / 100 g meat.6 - Sensory evaluation of beef patties showed that the treatment samples with peptides second peak of the Alcalase enzyme recorded the highest degree of sensory evaluation peptides compared with the first peak of the same enzyme and peptides peaks hydrolysate Pepsin enzyme.

انتاج مشتقات الكايتوسان الذائبة بالماء والمستخلصة من قشور الروبيان بتفاعلات ميلارد وتطبيقاتها في بعض الانظمة الغذائية == Production of Water soluble Chitosan derivatives extracted from shrimp shells by Maillard reaction and their application in some food systems

Author name: عاليه جميل علي
Supervisor name: علي حسين عبد الكريم العامري | منير عبود جاسم الطائي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study included the preparation of of chitin from shrimps shells Penaeus semisulcatus and studied of chemical composition of it like moisture,ash and protein wich was 7.80,0.43and3.50%respectively,The yield was 20.0%,then prepared three different types of Chitosan A,B and C from extracted chitin by removing of acetyl group from chitin at different times 4,10 and 20 hour, determination degree of deacetylation for types of Chitosan and standard Chitosanby FTIR 72.30,83.60,98.50 and85.20% respectively as was the viscosity of the three types of Chitosan 137.70,125.21 and74.11 centipoise respectively, selected Chitosan C,which carries ahigher valuedegree of deacetylation 98.50% and studied physico chemical and functional properties,which included (yield ,moisture , ash, protein and solubility) which amounted to16.20, 5.50, 0.20, 1.15 and 98.90%, respectively and reached a molecular weight (17.782) kilo daltone.Capacity of Chitosan to bind fat FBC and water WBC by using three types of oils are (olive oil, corn oil and sunflower oil) were estimated and were givin the highest capacity link with olive oil 665.0%, while the ability to bind Water amounting to 772%, as measured X - ray diffraction (XRD) and use the scanner electron microscope (SEM) to see morphological Chitosan and standard Chitosan Chitosan and the standard Chitosan, while the profile of the thermal gravimetric analysis (TGA) for Chitosan and standard Chitosan show that highest thermal decomposition at 308.56 ,315.92ᵒc respectively .The water soluble Chitosan derivatives were prepared by reaction with five types of reducing sugars by Maillard reactions and the use of three treatments,first treatment at a temperature of 50ᵒc for 1 - 7 days, the second treatment at a temperature of 100ᵒc for 1 - 7 hours and the third treatment at a temperature of 121ᵒc for 1/4 hour by autoclaving, ,and the properties of these derivatives were studied estimated yield, solubility, stability, free amino groups and the change in reducing sugars. highest value of yield gavein by The third - treatment, second treatment, first treatment reached 45.20,43.50,040.0% either solubility reached 1.10 ,0.780, 0.82 g/100 ml and derivatives stability at pH between10.50, 10.0, 9.80 respectively, The largest amount of amino groups and reducing sugars was the first treatment at absorbance 0.590 and0.352 respectively while the second an third treatment amounting to (0.201 and 0.060) respectively for amino groups , and 0.400 and 0.100 respectively for reducing sugars.Properties of antioxidants for prepared Chitosan and its derivatives. The antioxidant activity of Chitosan ranged between17.8 - 73.2%, while the highest activity for the third treatment of derivatives, the second and the first ranged of 76 0.81 - 85.88, 71.30 - 82.70 and 66.01 - 78.50% respectively, The reducing power of chitosan ranged between 0.320 - 0.700, while the highest value of absorbence of reducing power of derivatives to the Third treatment and then the second and finally the first which amounted to 1.290, 1.201 and 0.693 respectively, while the ability of chitosan to chelating ferrous ion and scavenging hydroxyl radical range between27.60 - 72.99%and16.90 - 84.9% respectively.Thehighest percentage to scavenging hydroxyl radical and ability to chelating ferrous ion by derivatives is shown by the third the second and the first treatment reaching 89.98 and 82.8 ,88.80 and 81.90, 84.80 and 76.01 % respectively, the selected third - treatment derivatives (121ᵒc for 1/4 hour) because of its best properties and carried the tests such as analysis of thermal gravimetric (TGA) that was noted three stages to different degrees heat to the disintegration of the derivatives, and the detection of toxicity on human bloodThird treatment compounds identified by GC - MS and the results showed that the derivatives have defferant compounds ,and identified compounds name, molecular formula, structural formula, molecular weight, size and retention time in addition to the profile mass spectrometerIn the practical side the use prepared chitosan in increasing storage time of table eggs, physicochemical tests were carried out for eggs stored at a temperature of 15 ᵒc and for aperiod of six weeks, it included estimation of weight loss (WL) wich amounted to 3.36% for chitosan at the six week as compared with acetic acid and control sample wich 9.01 and 8.96% respectively.Value of yolk Index YI of Chitosan - coated eggs decreased at the sixth week, amounted to 0.35, as compared with acetic acid and control sample wich reached (0.19 and 0.22) respectively, The value of Haugh Unit HU in the sixth week was higher to Chitosan treatment wich amounted to 61.42 compared with acetic acid treatment and control sample wich is24.95 and24.37 respectively.As regards eggs grading EG Chitosan treatment ongradiation A up to fifth week while acetic acid treatment and control sample reached gradiation C , when estimating pH of egg albumin pH high increased has been noted in control and sample treated by acetic acid in compared with chitosan - coated sample wich ranged 9.30, .Chitosan derivatives were also used chitosan as - natural antioxidants in beef products through the follow - up assessment peroxide value at different storage time the highest concentration 0.11g/100g beef meat displays the highest activity to prevent oxidation oil of beef meat wich reached5.11.Asensory evaluation has been carried of product stored on a 4ᵒc for 5 days of recipes sensory color,flavor,juiciness, tenderness and public acceptance using different concentrations.They have shown significant deferences ,but concentration at (0.11g/100g beef meat)was superior over the other concentrations and samples treated with BHT

معايرة معادلات التبخر نتح المرجعي لمنطقتي الفرات الاوسط وجنوب العراق وتاثير جدولة الري والتسميد في بعض خصائص التربة الفيزيائية وكفاءة استعمال المياه لمحصول زهرة الشمس Helianthus annuus L) == Calibration of Reference Evapotranspiration Equations in the Midmost Euphrates Region and Southern Iraq and the effect of irrigation schedule and fertilization on some Soil physical properties and water - use efficiency of sunflower crop (Helianthus annuu

Author name: طالب صبر حريجة العسكري
Supervisor name: داخل راضي نديوي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Theoretical foundations for research included the estimations of reference evapotranspiration (ETo) in the midmost Euphrates region and southern Iraq by applying seven mathematical models as well as CROPWAT 8.0 and CLIMWAT software using two sets of climatic data for periods 1970 - 2002 and 2004 - 2010.The results could be summarized as follow : - 1. The annual mean of ETo calculated by the Penman - Monteith equation (PM) for the meteorological stations of Nasiriya, Amarah, Semawa, Najaf, Diwaniya and Kerballa were (6.00, 6.20, 5.92,5.97,6.13,5.74) mm day - 1 respectively, during the first measurement period, and (6.47, 6.08, 5.86, 5.98, 6.13, 5.65) mm day - 1 respectively, during the second period. Also, the ETo estimated by CROPWAT 8.0 and imported by CLIMWAT software have showed the same trend of PM calculations with overestimate reached (6.10, 5.93) % during the first and secondperiods for CROPWAT 8.0 software, and underestimate for ETo reached 8.16 %during the first period for CLIMWAT software compared to PM calculations.2. The statistical criterions for evaluating the performance of six empiricalequations of the original versions were compared with the Penman - Monteithequation revealed that the variations in their performance with relativelypreference for temperature - based equations in comparison with radiation - basedequations. Blancy - Criddle (1950) equation (BC) has proved superiorperformance in Nasiriya, Semawa, Najaf, Diwaniya and Kerballa stations duringboth periods, also in Amarah station during the second period and declining itsperformance for the benefit of Kharrufa equation (1985) (K) during the firstperiod of measurement.3. The calibration process of the six empirical equations including : Hargreaves - Samani (1985) (HS), Makkink (1957) (M) , Priestley - Taylor (1972)(PT),andHargreaves (1975) (H) in addition to BC and K equations were made bysubstituting recalibrated constant values for the original constant values afterthese constant had modified to fit PM calculations and it used a set of statisticalcriterions to verify the validity of calibration and its ability to reduce theestimates errors compared to the original formulas. As the indices ofconcordance (D), confidence (C), correlation (r) and regression (R2) togetherwith the Nash - Sutcliffe Efficiency(NSE), standard deviation ratio (RSR). meanabsolute error (MAE), root mean square error (RMSE), relative error (RE)indicators and the ratio between both average estimations of ETo (r) are used toassess the validation of calibration and optimization which revealed thatconfidence became high in modified equations with reducing the estimation errorof ETo due to approach the values of these criterions from optimal value, then,they had improved efficiency of the six equations to simulate the values of EToare more accuracy than original formulae.4. The proposed new constants for the estimation of the ETo with : BC was 1.37for the time periods from January to April and 1.39 for May to December insteadof the original constant 1 : 41, HS was for the time periods from Octoberto March and for April to September instead of the original constantb0.0023, K was for June - July and for remained months instead of theoriginal constant 0.34, M are for the time periods from November to Mayand for June to October instead of the original constant 0.61, the equationsPT and H were 1.96 and 0 0.0165 for the period of December - May and 2.61and 0.0178 for the period from June to November instead of the originalconstants of 1.26 and 0.0135 respectively and equationsThe side applied to the study has included the implementation of the fieldexperiment for the cultivation of sunflower crop through the loop spring 2013 inprivate farm lies according to the geographical positioning system (GPS) withinthe coordinates (606,366.3437642), (606320, 3437729), (606353, 3437749),(606400, 3437660), is located in Batah town, Thi Qar province, at a distance of 17km west of the city of Nasiriyah, up 5 meters from the sea surface. The mainobjective of experiment included the following : i. Measuring ETo for the study through the crop growing seasonii. To determine the crop coefficient (Kc) and the actual water consumption(ETa) of sunflower crop in the Batah areaiii. . To asses effect of irrigation scheduling and fertilizer application as well astheir combinations on the growth indicators, nutrient uptake, dry matter,crop yields, the crop water use efficiency and water productivity.iv. Evaluating impact of irrigation scheduling and fertilizer application besidestheir combinations on some of soil physical properties at the end season.A field experiment was carried out using factorial experiment in a randomizedcomplete block design (RCBD) Layout with three replications in two factors, nineirrigation Scheduling treatments were assigned to the main plots and four fertilizertreatments were assigned to the subplots. Sunflower seeds (flame) were planted onMarch 23 with plant density 61538 plant ha - 1; the harvest operation was conductedon 18 / July / 2013.The irrigation Scheduling was achieved based on replenish root zone to theborders of field capacity when it reach the percentage of management allowabledepletion (MAD) identified about 55% of total available water (TAW), taking intoaccount the diversity of the effective depth of the root zone according to the stagesof growth, . The experiment involved Irrigation scheduling of the sunflower basedby calculating ETa using Kc which adjusted in situ multiplying by ETo thatestimated by PM equation (S1) ,original BC equation (S2) and adjusted K equation(S3) using meteorological data for real time that collected from agrometeorologicalstation nearby the field. PM equation (S6) using historicalmeteorological data and from pan evaporation with applied (FAO 56, 1998) tocalculate pan coefficient (S4). Whereas, irrigation Scheduling based on soil waterbalance according to daily measurements of soil moisture and adjusted daily onthe basis of changes in moisture content (soil water balance) (S5), fixed irrigationinterval and variable irrigation depth (S7), different irrigation interval and variableirrigation depth (S8) and soil water balance for bare soil (S9). Also the studyincluded four fertilization treatments were used ( F : mineral fertilizer, C : Compost,M : mineral fertilizer + Compost , N : non fertilization). The results showed thefollow : c1. The maximum value of ETo during season growth of sunflower crops accordingto field measurements has increased significantly when using evaporation pan,which gave the highest average rate of 8.81 mm day - 1 in comparison with PMequation that recorded the lowest value of ETo with mean equal to 8.00 mm day - 1,while BC and K gave 8.66 and 8.14 mm day - 1 respectively.2. The converged seasonal mean of Kc value for sunflower crops during growthseason were 0.864, 0.862, 0.863, 0.861, 0.862, 0.862, 0.861, 0.862 for S1 - S8treatments respectively, while the Kc varied in different stages of growth, thehigher value of Kc was 1.213 observed during the flowering stage, followed bytwo phases of vegetative growth and maturity with mean 0.796, 0.494respectively, whereas the lowest values (0.350) was recorded in the initial stage3. The ETa values were variable with various irrigation Scheduling treatments,the treatments (S1 - S8) gave mean ( 876.86, 938.98, 885.57, 943.40, 898.47,949.05, 835.61, 839.76) mm Season - 1 respectively, the mean values of ETa aredistributed as 52.28% during flowering stage, 23.38% through maturity, 18.97%during vegetative growth stage and 5.36% in the initial stage4. The Irrigation scheduling of the sunflower based on S5 treatment caused toincrement of water consumption by transpiration during flowering stage to reach83% from total ETa depth, whereas The Irrigation scheduling based on S7treatment caused to increment of water consumption by evaporation during thesame stage to reach 29% from total ETa depth.5. The results demonstrated that all Irrigation scheduling have been showed astrong relationship with water balance measurement (S5), by R2 is greater than0.91 and r greater than 0.95, also, the results showed there was a preference to therevised K equation to calculate ETa of sunflower crops in the region according toindicators of regression model each of the R2, slope and intercept which amountedto 0.9395, 0.9875, - 0.0144 respectively.6. The overall mean of net irrigation depth was 874.6 mm season - 1 ranged from941 mm season - 1 for S4 treatment to 800 mm season - 1 for S7 . the addition ofwater was 14 applications for S1 - S4 treatments and 15, 13, 12 applications for S5,S8, S7 respectively.7. The lowest nitrogen concentration in the leaves of Sunflower crops duringflowering stage related to decrease water supply at S7 (27.69 g kg - 1) , then, itincreased to maximum value ( 31.48 g kg - 1) when irrigation Scheduling based onS1 followed by S3, S5, S2, S4 treatments,. As respect with maturation stages theS5 treatment gave maximum mean (26.81 g kg - 1) was significantly superiorcompared to all treatments except S1 and it gave increment in nitrogenconcentration equal to 22% in comparison with S8 which recorded lowest nitrogenconcentration (21.94 g kg - 1). The results showed the F treatment was significantlysuperior followed by M treatment which gave average 34.43, 33.42 g kg - 1 duringflowering stage and 28.69, 26.48 g kg - 1 during maturation stage respectively.8. Increasing irrigation depth due to an application S4 and S6 treatments andIncreasing the irrigation frequency for S5 treatment led to significant increment inthe bulk density for 0 - 15 cm depth with mean 1.393, 1.384, 1.386 M m - 3respectively, the bulk density decreased steadily with decreasing of irrigationddepth and irrigation interval until reach their lowest level (1.350 M m - 3) at S7treatment. The bulk density was significantly decreasing when C and M treatmentswere applied with means of equal to (1.362, 1.364) M m - 3 respectively, atmagnitude 1% higher than their initial values. Also the results showed theirrigation scheduling treatments and fertilizer application as well as theircombinations failed to exhibit any significant influence on bulk density for depths15 - 30 cm and 30 - 45 cm.9. The results showed a significant increase in the soil penetration resistance forsoil with high watery supply (S4 and S6), while that status is significantlydecreased under treatment of low watery supply (S7, S8 and S1), the soils withmedium watery supply causing the pattern moderate between the two, also, thisepithet is significantly reduced when you add compost with an average of 708.09kPa, while there was a significant increase resulted from control treatment andaddition of chemical fertilizer alone, the proportion of 31.81 and 18.41%sequentially compared with the addition of compost.10. The results showed the possibility basic infiltration rate for most treatmentsunder the category (moderately slow) according to Landor (1991), by the mean ofbasic infiltration rate was 1. cm h - 1 ranged between 0. to 3. cm h - 1, whereasbasic infiltration rate for S4N and S9 decreased to 0.6 , 0.51 cm h - 1 respectively,thus it can be classified into (slow) while it increased greater than 2.0 cm h - 1 forS1C, S1M, S2M, S5C, S7F, S7M,S7C, S8M treatments which refer to moderateinfiltration rate according to the same classification . C and M fertilization led toimprove soil physical properties, which in turn positively affected the infiltrationrate with increment in magnitude (5 , 56) % respectively, compared to Ftreatment.11. Treatment of F gave a significant superior in most morphologicalcharacteristics and yield components, the efficiency of F treatment for productionwas 37.45 % followed by M and C treatments were achieved 24.66, 19.24 %respectively, compared to N treatment.12. Treatment F achieved highest yield of grains 1945.80 kg ha - 1, an increasing was24.32% in comparison with N treatment which gave lowest yield of grains reached1367.24 kg ha - 1, also there were a significant increase in crop water use efficiencyand water productivity are associated with application of F and M fertilization13. The productivity characteristics of sunflower were significantly affected byirrigation Scheduling treatments, The highest seed yield 1893.8 kg ha - 1and thehighest dry matter 5246.2 kg ha - 1were obtained under S5 treatment, while the S7treatment recorded lowest values of these indicators (1512.5 and 3765.4) kg ha - 1 respectively,14. The application of Irrigation scheduling based on S5, S1 and S3 treatments achieved a significant increase in crop water use efficiency and water productivity, the mean of first parameter was (0.219, 0.216, 0.216) kg m - 3 respectively, and themean of the second parameter was (0.228, 0.220, 0.219) kg m - 3 respectively.

تاثير حقن بيض التفقيس بفيتامين A وE وD3 وخليطهم في بعض الصفات الانتاجية والفسلجية والسلوكية لطائر السمان الياباني (Coturnix japonica) == Effect Of In Ovo Injection of Vitamin A , E , D3 and AD3E on some Productive , Physiological and Behavioral Traits of Japanese Quail )Coturnix japonica)

Author name: صلاح مهدي كاطع سلمان
Supervisor name: طارق فرج شوكت | قصي موسى جعفر
General topic: Agricultural sciences
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in the Animal Production field which belong to the Technical College - AL - Massaiab - AL - Furat AL - Awsat University which included two experiments the first was during 4/3/2014 until 22/3/2014, the second was during 22/3/2014 until 10/8/2014 and the to investigate the effect of injection of japanese quail eggs A,E,D3 and AD3E Vitamins on some productive , reproduction, physiological , behavioral and hematological traits .A total number of 720 eggs were used in the experiment ,those eggs were randomly distributed into six treatments , 120 egg in each treatment . The eggs were injected before the first day of incubation in the air sac by vitamins. Treatments were arranged as fallow : T1 : Negative control treatment .T2 : Positive control treatment , it included injection of 50 microliter of corn oil for each egg.T3 : It included injection of 50 microliter per egg by vitamin A (100 IU/egg).T4 : It included injection of 50 microliter per egg by vitamin E (15 IU/egg).T5 : : It included injection of 50 microliter per egg by vitamin D3 (100 IU/egg).T6 : : It included injection of 50 microliter per egg with the blend of AD3E vitamins by the same concentration per egg.The most important results recorded in the first experiment were high significant increased (P≤0.05) of hatchability of fertile egg and post chick weight but not effect significant on embryo mortalities during embryogenesis .The second experiment included rearing of the post hatched chicks of the first experiment in wooden dataries , chicks were all randomly allocated inSummarybto three replicates for each treatment (17 chick / replicate) to study productive , reproductive , physiological, behavioral , histological , biochemical and hematological traits for Japanese quail.Results illustrated the followings : 1 - A significant increase (P≤0.05) of productive traits in treatments of injection (body weight , weight gain , feed utilization , feed conversion ratio ) and find significant increase egg production , egg weights and mass as well as shell characteristics (egg shell weigh and thickness ) in Treatments (T5 , T6 ) comparisons with the negative and positive control treatments .2 - A significant effect (P≤0.05) on early maturation for males and females in treatments of vitamins injection , more over there were a significant increase of their body weights during maturation and early behavioral jumping with a significant surpass of cloaca area in comparison with males in control treatments.3 - A significant increase (P≤0.05) of relative weights of testes in males of all ages in injection treatments with a significant increase of seminal tubules diameters and thickness of germinal layer of testes during 28 and 47 days in comparison with that of the negative and positive controls.4 - A significant increase (P≤0.05) of relative weights of ovaries with an increase of the primary follicle diameter in females of injection treatments during 28 , 47 and 69 days in comparison with that of the control .5 - A significant increase (P≤0.05) of relative weight of fabricia gland and spleen for both sexes of birds in injection treatments , more over the interaction between vitamins injection and sex was significant in all ages.6 - A significant increase (P≤0.05) of PCV in injection treatment , moreover the PCV was increased in females at 28 and 47 days, the interaction between vitamins injection and sex was significant in all ages .7 - No significant increase (P≤0.05) was noticed in Hb of injection treatments at 28 but there were a significant increase (P<0.05) in 47 days in vitamins injection treatments and there was a significant increase (P≤0.05) of Hb in 69 days in the sixth treatment in comparison with the control treatments, and it was numerical on other treatments ( 3rd, 4th and 5th) there was no interaction between vitamins injection and sex at 28 days but the interaction was significant in at 47 and 69 days .8 - There were no significant increase (P≤0.05) of RBC in injection treatments at 28 days but it was significant at 47 days in the third and sixth treatments , at 69 days there was a significant increase of RBC in injection treatment as compared with the control treatments , the interaction between injection of vitamins with sex was not significant at 28 days but it was significant at 69 days .9 - There were no significant effect (P≤0.05) in WBC in vitamins injection at 28 , 47 , 69 days as compared with the control treatments moreover there was no significant interaction between injection treatment and sex.10 - There were significant increase (P≤0.05) in LH , FSH and testosterone levels in serum of vitamins injection treatment for both sexes , the interaction between treatment sex was significant in all ages .11 - There were a significant surpass (P≤0.05) of total protein level in injection treatments at all ages as compared with the control treatments and the interaction between treatments and sex was significant at all ages . glucose and cholesterol level were not affected according to the treatment but females were significant surpasses on males at all ages . The interaction between treatment and sex was not significant at all ages12 - There were a significant surpass (P≤0.05) of Ca and P in injection treatment at all ages as compared with control treatments , the interaction between treatment and sex for Ca and P level was significant at all ages .13 - The study revealed no significant improvement in concentration Ca , P and ash in tibia bone , weight and length of thigh for both sexes in injection treatment at all ages .

تاثير انظمة الاضاءة والتقنين الغذائي الزمني في بعض الصفات الانتاجية والفسلجية والتناسلية لطائر السمان الياباني (Coturnix japonica) == The Effect of Lighting Systems and Feed Restriction timing on Some Productive, Physiological and Reproductive Traits of Japanese Quail (Coturnix japonica)

Author name: صباح كاظم مرزوق الحمود
Supervisor name: ریاض كاظم موسى | ربیعة جدوع عباس
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تصميم وتصنيع وتقييم الاداء الميكانيكي لالة زراعية من خلال وضع السماد العضوي في خنادق تحت سطح التربة وتاثيرها في بعض خصائصها ونمو وحاصل الذرة الصفراء Zea mays L == Designing , constructing and evaluating the mechanical performance for agricultural machine laying the manure under the soil surface and its effect on some of soil properties and growth and yield of corn ( Zea mays L.

Author name: صادق جبار محسن
Supervisor name: شاكر حنتوش عداي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: ditch opener and manure laying machine was designed and manufactured in the agricultural machines and equipment dept., Agriculture college, Basrah university in 2015. This machine was evaluated to determine its mechanical performance and its ability in laying manure in ditches under the soil surface as well as the effect of the added manure on the soil physical and chemical properties, corn crop growth and production parameters.The machine consists of a frame, manure tank, chemical fertilizers tank, subsoiler for soil disturbance down to 80 cm, two boards for soil digging out to open the ditch , two boards to return the soil to ditch to bury the manure , manure and chemical fertilizers feeding mechanisms and blades for manure mixing with the soil. Also its provide with two pipes to transfer the chemical fertilizers to the soil and a petrol engine to operate the two feeding mechanism.The machine disturbed the soil by the subsoiler and digging different ditches of different depths by two boards . In additional to that it can lay the manure at different levels in the opened ditches using the feeding mechanism and thereafter buried the manure in the soil . The manure can be laid under the soil as separate layer or mixed with soil using the mixing blades.Three experiments were conducted to evaluate the machine performance. These experiments are : (1) - Laboratory experiments were conducted to study the effect of the machine forward speeds (0.32, 0.46 and 0.63 m sec - 1), manure feeding mechanisms rotating speeds (360, 440, 560, 710 and 880 rpm) and two manure feeding openings areas (0.024 and 0.048 m2) as well as the interaction of the above parameters on the amount of the manure per hectare (ton ha - 1). These results were analyzed using CRD and the statistical program (Genstat). Mean results were compared using least significant difference (RLSD) at 0.05 level.The results showed the manure amount per hectare significantly increased as the rotating speeds of the feeding mechanism increased,Bthe forward speeds decreased and the feeding opening areas increased. The highest value was recorded for forward speed of 0.32 m sec - 1 , rotating speed of 880 rpm and feeding open area of 0.048 m2. These means are 64.17, 62.06 and 54.06 ton ha - 1 respectively. while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and the feeding open area of 0.024 m2 gave the lower values which they are 26.91, 30.04 and 33.75 ton ha - 1 respectively. Also, the forward speed of 0.32 m sec - 1 , the rotating speed of 880 rpm and the feeding area of 0.048 m2 recorded the highest amount of manure per hectare (110.00 ton) while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and manure feeding area of 0.024 m2 recorded the lowest value of manure per hectare (4.00 ton).(2) - A field experiment was conducted in silty loam soil in the agriculture college field at Garmit Ali location to study the effect of the implement operation depths (10, 20, 30, 40 and 50 cm), the angle between the soil digging out boards (45⁰ and 60⁰) two soil types namely cultivated soil before one year and uncultivated soil and their interaction on the implement mechanical performance parameters . These parameters are the draft force, soil disturbed area and the energy utilization efficiency (EUE) as well as the draft force requirement of the implement units (subsoiler, ditch opening boards, soil returning boards and soil mixing blades). The results were analyzed using RCBD. The analyzing was carried out by the statistical program (Genstat). Mean results were compared using RLSD at probability level of 5% , and showed the followings : (2 - 1) - The draft force, the disturbed area and the energy utilization efficiency increased significantly when the operating depth increased from 10 to 50 cm. The amount of the increase is 26.66 kN , 0.4111 m2 and 7.67 m3 mJ - 1 respectively.(2 - 2) - The angle between the opening boards of 60⁰ significantly surpassed angle of 45⁰ in increasing the draft force, the disturbed area and EUE. The percentage of increase is 12.55 , 21.93 and 10.85 % respectively.C(2 - 3) - The draft force decreased while the disturbed area and EUE increased by 16.30, 14.91 and 20.72 % in the disturbed soil compared with undisturbed soil respectively.(2 - 4) - The draft force requirement of the subsoiler was higher than that for the other implement parts. The share of the draft force requirement of the subsoiler was 60 % out of the total draft force requirement of the implement while it was 26, 9 and 5 % for the ditch opening boards, the soil returning boards and soil mixing blades respectively.(2 - 5) - The draft force requirement of all implement units increased significantly with implement operating depths and the angle between the ditch opening boards and it was higher in the uncultivated soil compared with the cultivated soil. The draft force requirement of the soil returning boards and the soil mixing blades in both soil types was not significantly different.(3) - Another field experiment was conducted in the field of the agriculture college at Garmit Ali location in the season of 2015 in a cultivated soil of silty loam texture. The aims of the experiment were to study the effect of the added manure level (0, 20 and 40 ton ha - 1), the depths of added manure (10, 20 and 30 cm) and the methods of the addition (subsoil layer and mixing with soil) and their interaction on the soil properties and corn crop (Zea mays L.) growth parameters and yield at the end of the growth season.The soil was plowed and harrowed (soil clods breaking down). The manure was added thereafter by the implement in the soil according to the studied treatments. The corn seeds were sown in rows at 1/7/2015. The drip irrigation method was used to irrigate plants until the end of the experiment 10/10/2015. The other agricultural operations were conducted according to methods used in the around area.Soil and plants samples were collected for analysis and the corn seeds yield was calculated at the end of the growth season. The measurements included soil bulk density, soil moisture content, soilDsalinity, soil pH, the dry weight of the shoot, N, P, K uptake in shoot and the total seeds production.The split - split plot in RCBD design was used to analyze the means using Genstst program. Mean results were compared using RLSD at probability level of 5 %, and showed the followings : (3 - 1) - The manure level of 40 ton ha - 1 surpassed the other two levels (0 and 20 ton ha - 1) in giving higher values of soil moisture content, higher uptake of N, P, K, a greater amount of dry weight and total yield while it gave lower soil bulk density, soil salinity and soil pH.(3 - 2) - Increasing the depth of added manure from 10 to 30 cm with the mixing method lead to significant increase in the soil moisture content, uptake of N, P, K, the dry weight and the total yield production. Whereas the soil bulk density, soil salinity significantly reduced while the soil pH was not significantly affected.(3 - 3) - Mixing of 40 ton manure ha - 1 at depth of 30 cm gave the highest crop growth and yield production which they are 13.94 and 6.75 ton ha - 1, respectively.

عزل وتشخيص بكتريا Bacillus cereus واستعمالها في انتاج متعدد هيدروكسي البيوترات == Isolation and Identification of Bacillus cereus Bacteria and Using in Polyhydroxybutyrate Productio

Author name: شيماء ذياب جدوع السهلاني
Supervisor name: امال كاظم غضبان الاسدي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Forty three local bacterial isolates were obtained after heating treatment for different sources collected from many places in Basra government. Included : Vegetables, Fruit, Pastry (cakes), Legumes, Soils, Sand, Animal manure, the papyrus plant, milk, water liquefaction and sewage water. Primary screening for the isolates by Sudan black B dye only. 22 isolates were chosen with strong staining, and the identification of all the isolates revealed were belong to genus Bacillus by studying microscopic and biochemical tests. They were : 4 isolates Bacillus cereus, 2 isolates Bacillus firmus, 2 isolates Bacillus lichenformis , 3 isolates Bacillus megaterium, 4 isolates Bacillus mycoides , 3 isolates Bacillus pumilus and 4 isolates Bacillus subtilis.Secondary screening of these isolates was Polyhydroxybutyrate (PHB) produced, Bacillus cereus B5 given 2.4 g / L the highest production of PHB. A PCR technique was used for 16S rRNA test and detecting the gene of PHB production in Bacillus cereus B5.The highest PHB production from Bacillus cereus B5 was 6.2 g / L, biomass 8.4 g / L and yield 73.8% the by using optimum conditions : incubation temperature 35̊ C, for 48 hours aerobically by using shaking incubator for 150 RPM/ min, 2% inoculum volume, the pH was adjusted to 7, and the production media where contained 1% glucose as carbon source and peptone as a nitrogen source. 3% have dated juice was the best substitute for glucose as carbon source, it gave 7.11 g PHB / L and the yield 79 %.Analysis with FT - IR was showed, that PHB produced from date juice media had a peak at 1723 cm - 1 this means it belong to an esterpolymers group. And GC - MS showed 12 compounds produce from analyzing PHB as short chains of fatty acids.The properties of PHB produced from date juice media were : The degradation temperature was 312̊ C, the percentage of crystallization was 60%, molecular weight was 423.674 KDa., permeability of water vapor was 56.2 ×10 - 8 g / Pa.s.m2, tensile strength was 24.9 MPa, the blend 40% polyethyleneglycol as plasticized with PHB increased the percentage of elongation to 8.7%. PHB is non - toxic and without hemolysis on human blood.Studying biodegradation of the plastic films of PHB by using bacterial isolates were E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus in petri dish showed that all isolates can degrade PHB, biodegradation in soil and at soil surface was 100% after 28 days.PHB packages increased the shelf life of strawberries and grapes after storage for 15 days, compared with polyethylene packages, and It was reduced the numbers of microorganisms in butter, the percentage of free fatty acids and peroxide value compared with butter with polyethylene after 15 days

تاثير اضافة النتروجين في امتصاص NوPوK وتوزيعها في اجزاء النبات ونمو وحاصل ثلاثة اصناف من الحنطة Triticum aestivum L. == EFFECT OF NITROGEN APPLCATION ON N, P, K UPTAKE AND DISTRIBUTION WHIHIN PLANT PARTS, GROWTH AND YIELD OF THREE WHEAT CULTIVARS Triticum aestivum L.

Author name: سندس عبد الكريم محمد العبد الله
Supervisor name: عبد المهدي صالح حسين الانصاري | وليد عبد الرضا السباهي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A field experiment was conducted at Al - Daire site 40 km north of center Basrah Governorate .The study was conducted to investigate the effects of different nitrogen fertilizer levels on dry matter accumulation, (N, P, K) uptake, distribution in different parts of three cultivars of wheat plants and their yield during two seasons (2011 - 2012 & 2012 - 2013).The experiment was split - plots in R.C.B.D design with three replicates, Nitrogen rates (0, 60,120 and 240Kg ha - 1 occupied the main plots, while (IPA - 99, Abu - Graib and Latifia) Cultivars Occupied the sub - plots. Samples of wheat plants were collected at 10 growth stages : seedling stage, tillering (main shoot and 1 tiller), tillering (main shoot and 4 tillers), stem elongation, booting, 50% of spike emergence, 50% flowering, milk stage, dough stage and maturity. Each plant sample was separated into different plant parts : blades, sheathes, stems, flag leaves, spikes and seeds. These parts were dried at 70°C and dry weight of each part was recorded and sub sampled for chemical analysis of N , P , and K. Growth parameters (No. of day till 50% flowering , No. of day from 50% flowering to Maturity , flag leaf area , plant height, spike length and No of tillers m - 2) , yield components (No. of spike m - 2, No. of grain in spike and weight of 1000 grain ),grain yield, biological yield, harvest Index, grain protein percentage and nitrogen use efficiency were also studied.Results showed that dry matter accumulation in plants of all cultivars did not differ at early growth period, While after the growth stage of 50% flowering plants dry weight of Cv.IPA - 99 was higher than other cultivars, This difference in dry weight continue till maturity stage at which total dry weight were 14473.58, 13373.96 and 12743.59 Kg ha - 1 for IPA - 99, Abu - Graib - 3 and Latifia respectively.Dry matter yield were not affected by N levels during the early growth period ,then after dry weight of plants increased as N rates increased ,total dry weight were10752.61, 12737.49 and 15347.10 Kg ha - 1for 0, 60 , 120 Kg Nha - 1 respectively, with no differences between 120 and 240 Kg Nha - 1 levels.Dry matter of plant parts differed among cultivars, with IPA - 99 having the highest results. Increasing nitrogen rates increased weight of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.IIResults also showed reduces in dry weight of different plant parts(except grains)after reaching a maximum weigh at dough stage for Spikes, and 50% flowering for the other parts. The highest decreased was in blades and lowest in sheathes. The application of N fertilizer decreased translocation of dry matter from plant parts to grains. Data showed that no differences in nutrient uptake were observed for cultivars till ending of tillering stage, but after this stage IPA - 99 cultivar showed highest uptake till the end of the season. Nitrogen uptake was157.68,136.72,124.51 Kgha - 1 ,P uptake 19.70,16.93,15,67 Kg ha - 1, K uptake136.30,121.10,112,60 Kg ha - 1 for IPA - 99,Abu - Graib - 3 and Latifia, respectively.Increasing N rate increased nutrient uptake of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.uptake of N, P, and K for all cultivars and nitrogen rates declined as the season progressed, this decline differed among nutrients and plant parts.Grain contained 59.20, 79.00 and 7.45% of total nutrient N, P and K uptake, whereas vegetative parts contained 40.80, 21.00 and 92.55% of total nutrient uptake, respectively.Cultivar IPA - 99 resulted in highest grain yield among studied cultivars. Increasing N rate from 0 to 120 Kg N ha - 1 only increased grain yield, the rate 240 KgNha - 1 did not affect yield significantly as compared with that of 120 Kg N ha - 1.Results also showed a significant interaction between Cultivars and N rates. The highest grain yield obtained at treatment IPA - 99×240 Kg N ha - 1with no significant differences than IPA - 99×120 KgNha - 1.Results indicated that highest nitrogen use efficiency (66.23%) was found in plant of IPA - 99 cultivar, whereas, the lowest efficiency (47.37%) was found in plant of Abu - Graib - 3 cultivar. At all cultivars increasing N rates to 240 KgNha - 1 decreased nitrogen use efficiency.Therefore it can be concluded that 120 Kg Nha - 1 can be recommended as the best rate for N to all cultivars under similar growing conditions

تقييم المضافات الغذائية في بعض المنتجات الغذائية المحلية ودراسة تاثيراتها الكيميائية والفسلجية والنسيجية في الفئران المختبرية == Evaluation of food additives in some local food products and study their chemical, physiological and histological effects in laboratory mice

Author name: رنا داود سلمان الكامل
Supervisor name: علي احمد ساهي | قاسم فوزي عبد الكريم
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Due to the progressive expansion in the use of chemical additives in food processing associated with absence of quality control and loss of proper application of the international standards of food additives, making human beings exposed to high risks from ingestion of these foods which might be so mild in cases like simple poisoning and more complex like cancers, the present study was performed, which includes four axes : The First Axis : 1 - A questionnaire study of food additives : the results of which indicated that 55.42% of consumers eat packed food continuously, and most of them(61.44%) have no interest in reading the labels on the outside cover of the packaging , despite the fact that 49.39% of them consume it daily.Whereas 51.80% of consumers had no specific knowledge or familiarity about food additives, 54.14% of them do not possess any knowledge about their side effects and 77.10% of them did not know the symbols listed out.2 - A survey of some food products that used sodium benzoate as a preservative in their manufacture. The results indicated that 163 types of food samples contain sodium benzoate, including different products such as soft drinks, canned juices, sherbet, jams, tomato sauce, pickles, ketchup and ambah.3 - A survey of some food products in which sugar enters in their composition, These include 231 products which mentioned the word sugar or artificial sugar within labels on the outside without mentioning its kind .These products include : imported ice cream, jams, candies, soft drinks and diet drinks, cake, sherbet , fruit preserved in sugar solution and biscuits. 4 - A questionnaire study of the market sales of locally made pickles. The results indicated that : 71.79% of consumers frequently ate pickles in large quantities despite the fact that 48.71% of them does not have any information about sodium benzoate preservatives added to some of its , whereas 64.10% of them have no information about its side effects that result from the ingestion of these products beyond the allowable limits. 5 - A questionnaire study for the market sales of locally made ice cream.The results showed that : 77.77% of consumers ingested ice cream in large quantities despite the fact that 63.88% of them did not have any information on the sweetener saccharin added to some of its. While 62.50% of consumers possess no information about the side effects resulting from consumption of products that contain artificial sweeteners used in food processing especially when there is more than the allowablelimit.

اثر اضافة الكبريت الزراعي واليوريا والسوبر فوسفات في نشاط انزيمي اليوريز والفوسفاتيز وحاصل الذرة البيضاء Sorghum bicolor L. == Effect of Agricultural Sulfur , Urea and TSP application on the activity Of Urease phosphatase and yield of (Sorghum bicolor L. )

Author name: رشاد عادل عمران الزبيدي
Supervisor name: هيفاء جاسم حسين التميمي | عبد الزهرة طه ظاهر
General topic: Agricultural sciences
Specific topic: Soil and Water - Fertility
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

المسؤولية الادارية عن الاعمال الطبية : دراسة مقارنة

Author name: زياد خالد يوسف المفرجي
Supervisor name: رعد ناجي الجدة
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The subject of the administrational responsibility of medical works deals with the system of administrational works concerning the medical aspect. As it is well known, theadministrational law is a judicial law in which justice plays a big role in establi

الهيئات المستقلة وعلاقتها بالسلطة التشريعية في العراق : دراسة مقارنة == The Relationship of The Independent Institutions With The Legislative Authority And Their Applieations In Iraq A Comparative Study

Author name: هشام جميل كمال ارحيم
Supervisor name: عامر عياش عبد بشر الجبوري
General topic: Law
Specific topic: Constitutional Law
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تعد الهيئات المستقلة احدى الاجهزة التنظيمية المستقلة في الدولة والتي تم استحداثها بموجب دستور جمهورية العراق لعام 2005 النافذ، وتم منح الهيئات المستقلة اختصاصات واعمال متنوعة، لذلك وجب علينا دراستها لمعرفة الاطار النظري للهيئات المستقلة وتحديد الهيئات ا | The independent institutions are regarded as independent organizations within the government; it was renewed in 2005 according to the Iraqi constitution of the same year. Those institutions have their own work and specializations, and for that reason it i

الحقوق السياسية لمتعددي الجنسية == Political Rights For Multinationals

Author name: روافد محمد علي الطيار
Supervisor name: علي هادي حميدي الشكراوي | فراس كريم شيعان
General topic: Law
Specific topic: Private International Law
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:
Abstract: The contribution of the individual in political life is based on a fundamental requisite to enjoy the nationality of the State which purports to exercise their political rights on its territory, but that he may enjoy the individual more than the nationali

الحماية الجنائية للاطباء : دراسة مقارنة == The Criminal Protection For Doctors A Comparative Study

Author name: نسرين محسن نعمة الحسيني
Supervisor name: علي حمزة عسل الخفاجي
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:
Abstract: الحماية الجنائية للاطباء تتمثل بالنصوص التي يقررها المشرع الجنائي لاحاطة الاطباء بالحماية التي يراها كفيلة برد الاعتداءات والافعال غير المشروعة التي قد تؤدي الى النيل من حياتهم او سلامتهم الجسدية او شرفهم وذلك بتجريم كل من يعتدي عليهم ومعاقبته والاقتصاص م | The Criminal Protection for Doctors (A comparative Study)Criminal protection for physicians are texts decided by the criminal legislator to inform doctors the protection seen enough to chill all kinds of attacks and illegal acts that may lead to the Nile

نظرية العلم بالتجريم

Author name: عقيل عزيز عودة
Supervisor name: جمال ابراهيم عبد الحسين الحيدري
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: العلم هو حالة ذهنية ونفسية لا تدرك بالحس الظاهر تعبر عن انكشاف الصور في الذهن من دون اقترانها باحكام. والعلم عنصر مهم لتوافر الركن المعنوي للجريمة لانه لا ارادة بغير العلم، فالعلم لا غنى عنه في تكوين القصد او الخطا، فالقصد الجرمي ما هو الا علم وارادة يحيط | Knowledge is a mental and psychological case that is not felt by apparent senses. It expresses images disclosed in mind without its association with decisions. Knowledge is an important element due to the availability of the subjective element of the crim

الاسناد في القاعدة الجنائية : دراسة في القاعدة الجنائية الموضوعية == The Attribution In The Criminal Rule A Study of The Criminal Rule

Author name: حيدر غازي فيصل الربيعي
Supervisor name: تميم طاهر احمد الجادر
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The basic rules stipulated in the modern criminal legislation is that no crime and no punishment without a law or according to a law. The meaning of that is the criminal is the main means to which the legislator resorts if he is to criminalize certain beh

التوفيق بين احكام التجريم والعقاب وثوابت احكام الاسلام : دراسة مقارنة == Matching Between Rules of Criminalization And Penalization And Constants of Islamic Rules Comparison Study

Author name: حيدر حسين علي الكريطي
Supervisor name: حسون عبيد هجيج
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:
Abstract: تعالج الاطروحة مشكلة دقيقة ومعقدة متشعبة الجوانب ومتعددة المستويات تتمثل بتعارض احكام جوهرية اساسية يضمها التشريع الجنائي العراقي مع القاعدة الدستورية المحورية التي رسخها دستور جمهورية العراق لعام 2005 وهي : ( عدم جواز سن قانون يتعارض مع ثوابت احكام الاسل | This study tackles with a minuet, complex and ramified - sides problem with multi levels are represented by conflicting of major essential laws included by Iraqi criminal legislation with the constitutional central base that was installed by the Iraqi con

التحقيق البرلماني في الانظمة السياسية البريطاني والامريكي والمصري والعراقي : دراسة مقارنة == The Parliamentary Investigation Comparative Study In UK, USA, Egypt & Iraqi Regimes

Author name: رياض محسن مجول
Supervisor name: حميد حنون خالد الساعدي
General topic: Law
Specific topic: Constitutional Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
Key words:
  • رقابة برلمانية
  • تحقيق برلماني
  • لجان التحقيق
First pages:
Abstract: استقر العمل في مختلف الانظمة السياسية على امتلاك البرلمان وسائل عدة لمراقبة اعمال الحكومة والوقوف على مدى صحتها واتفاقها مع الصالح العام، فظهر السؤال والاستجواب والتحقيق البرلماني بوصفها ابرز الوسائل التي يملكها البرلمان لمراقبة اعمال الحكومة، وقد تميز | The parliamentary investigation has been consider one of the most important instrument that a parliament have it to control the action of executive authority in the state because it's characterized from its like legal control instrument with many characte

اثر الحكم الصادر بعدم الدستورية : دراسة مقارنة == The Effects of The Judgment of Unconstitutionality Comparative Study

Author name: احمد علي عبود الخفاجي
Supervisor name: رافع خضر صالح شبر
General topic: Law
Specific topic: Constitutional Law
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:
Abstract: The constitutional judiciary controls laws with a view to ensure respect for the rules in the constitutional document of the most important legal means to ensure enforcement of the law and properly implemented, they are designed to safeguard the constitut

دور الاقليات في حكم العراق وفقا لدستور 2005

Author name: ايات سلمان شهيب السعدي
Supervisor name: صعب ناجي عبود
General topic: Law
Specific topic: Constitutional Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

دور الضبط الاداري البيئي في حماية جمال المدن : دراسة مقارنة == The Role of The Environmental Administrative Control For Protection The Beauty of The Cities Comparative Study

Author name: سجى محمد عباس الفاضلي
Supervisor name: غازي فيصل مهدي
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: Background : Beauty secret of Allah power which is innate in humans realize sense and heart through sensory perceptions that God gave us. The beauty is essential in human life. Became the attention in the beauty of cities and glory through the protection

التنظيم القانوني للمحكمة الادارية العليا في العراق : دراسة مقارنة == The Legal Organization of The Supreme Administrative Court In Iraq A Comparative Study

Author name: ميسون علي عبد الهادي الحسناوي
Supervisor name: حنان محمد مطلك القيسي
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The modern arrangement of the contemporary state requires the distribution of specializations among ministries, offices, and foundations so that the latter can devote themselves to accomplish their works successfully and also the mobilization of efficien

النظام القانوني للهيئات المستقلة : دراسة مقارنة == The Legal System To Independent Bodies Comparative Study

Author name: صادق محمد علي الحسيني
Supervisor name: اسماعيل صعصاع غيدان البديري
General topic: Law
Specific topic: Constitutional Law
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:
Abstract: The idea of independent bodies, which prevailed in many of modern legal systems, considered as modern concept. however the purpose of establish its in order to achieve most important functions deals with public freedom, economic action or transparency as

المسؤولية المدنية الناشئة عن الاعتداء على الحق في الصورة : دراسة مقارنة

Author name: حسن محمد كاظم المسعودي
Supervisor name: جاسم العبودي
General topic: Law
Specific topic: Civil Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

تعدد المسؤولين واثره على ضمان حق المتضرر : دراسة مقارنة == Multitude of Responsibles And Its Effect on Guaranteeing The Right of The Damaged A Comparative Study

Author name: غني ريسان جادر الساعدي
Supervisor name: عزيز جواد هادي الخفاجي
General topic: Law
Specific topic: Civil Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

المحكمة الجنائية الدولية لسيراليون == The International Criminal Tribunal For Sierra Leone

Author name: مازن عثمان محمد الجميلي
Supervisor name: حكمت محمد شبر
General topic: Law
Specific topic: International Criminal Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
Key words:
  • المحكمة الخاصة
  • نورمبرغ
  • طوكيو
  • يوغسلافيا
  • رواندا
  • سيراليون
  • الابادة الجماعية
  • الجنود الاطفال
  • التهجير القسري
  • اتفاقية لومي
  • شارلزتايلور
  • ليبيريا
First pages:
Abstract: ان هذا الموضوع يعد من المواضيع المهمة في القانون الدولي العام حيث ان عنصر الجزاء في القانون الدولي العام يبرز هنا وبكل وضوح وهذا النوع من المحاكم ليس وليد اليوم حيث يذهب بعض الباحثين الى ان اول تطبيقات القضاء الدولي الجنائي ترجع الى العصر الفرعوني القديم | The International Criminal Tribunal for Sierra LeoneThis issue is one of the important topics in public international law as a punishment in public international law highlights here and very clearly, this type of courts is not a new phenomenon which goes

الاثار التبعية للعقوبة التاديبية في القانون العراقي والقوانين المقارنة

Author name: كوثر حازم سلطان
Supervisor name: عدنان العابد
General topic: Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
Key words:
  • الموظف العام
  • General employee
  • العقوبة التادبية
  • Disciplining punishment
  • الترقية
  • Promotion
  • العلاوة
  • Bonus
  • الحوافز
  • Rewards
  • المعنوية
  • Morale
First pages:
Abstract: The Organizational basics in various systems works for rewarding the qualified employee on his good administrational behavior with rewards, encouraging bonuses, promotions, and others, beside of that the ignoring or perverted employee will be punished wit

الجرائم الماسة بحرية التعبير عن الفكر

Author name: نوال طارق ابراهيم العبيدي
Supervisor name: جمال ابراهيم عبد الحسين الحيدري
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The intellectual freedoms is from the most important freedoms which the human needs in his life for connecting in spiritual sides which allow in forming his opinions and thoughts in different issues these freedoms include, freedom of belief, freedom of ex

نظرية الفعل غير المشروع دوليا : دراسة في المسؤولية الدولية == Theory of Internationally Wrongful Act : Study In International Responsibility

Author name: محمد محمود امين
Supervisor name: عصام عبد الرزاق العطية
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: مما لا شك فيه ان فاعلية اي نظام قانوني يتمثل في مدى نضوج قواعد المسؤولية فيه، وفي ميدان القانون الدولي العام فان قواعد المسؤولية فيه تلعب دورا اساسيا ومهما كونها تشكل احدى الضمانات المهمة والرئيسة لحسن تطبيق قواعد القانون الدولي، وكفالة احترام الدول | The functionalism of any legal system depends upon the extent of maturity of the rules responsibility embedded in it. In the filed of public international law, the rules of responsibility play a basic and important role as they form one of the basic guara

السلطة التقديرية للادارة في مجال الضبط الاداري في الظروف العادية : دراسة مقارنة في القانون الوضعي والشريعة الاسلامية == The Evaluation Authority At Field of Management Accuracy In The Normal Conditionscomparison Study In The Positive Law And Sharia Islamic Law

Author name: مهند قاسم زغير
Supervisor name: حيدر طالب الامارة
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The compliance of the Management to the law means that it respects the legal rules derived from various sources; however it does not meet the requirements of the public interest. It required leaving a kind of discretion to the Management in some cases in

الجرائم الناجمة عن الغزو العسكري == Crimes Resulting From Military Invasion

Author name: ادم سميان ذياب الغريري
Supervisor name: عبد القادر الشيخ | صباح مصباح محمود
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تتركز هذه الدراسة المتخصصة في القانون الجزائي بالجرائم الناجمة عن الغزو العسكري في تقديمها طرحا قانونيا عنها، بنبذة عما مرت به البشرية من غزوات في العصور القديمة والوسطى والحديثة، واعطاء تعريف قانوني للغزو وتمييزه عما يشتبه به من صور الحرب الاخرى : | This study, specialized in criminal law, concentrates on crimes arising from military invasion, starting with a brief of what passed by humanity invasions across ages, giving a legal definition of invasion and distinguishing it with what is suspected of

رقابة الادارة في مرحلة تنفيذ العقد الاداري == Administration Censorship At The Stage of Execution To The Administrative Contract

Author name: هنادي فوزي حسين
Supervisor name: غازي فيصل مهدي
General topic: Law
Specific topic: Administrative Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: some provisions, concerned with monitoring of the making of administrative contract, on the other hand. Management control (monitoring) importance in the phase of implementation of administrative contract, stems from the significance and gravity of probl

دور محكمة العدل الدولية في تسوية منازعات الحدود البحرية

Author name: فاطمة حسين شبيب
Supervisor name: اكرم داود الوتري
General topic: Law
Specific topic: Financial Legislation
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: اولا : ملخص باللغة العربية : للامتدادات البحرية للدولة الساحلية اهمية اقتصادية لما تحويه من ثروات طبيعية فضلا عن الناحية الامنية التي تشكل عاملا مهما في حماية امن وسلامة اقليم الدولة البري والبحري.ونتيجة للتغيرات الواسعة التي شهدها قانون البحرالاسيم | Disputes concerning maritime frontiers among states, are one of the important subjects of international law. According to article (2/3) of the United Nations charter, states is compelled to settle their disputes peacefully. By recoursing to one of the pea

الجنسية في العلاقات ذات الابعاد الدولية الخاصة : دراسة مقارنة == The Nationality In The Relationships Concern International Private Dimensions : Comprative Study

Author name: عبد الرسول عبد الرضا جابر الاسدي
Supervisor name: رشيد مجيد محمد الربيعي
General topic: Law
Specific topic: Private International Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: The idea of the subjet of the research is divided into three chapters.The first chapter discusses the nationality and its'effects in moving the international character of relation ships, the notionality in this scope appears as an instrument to dis Tingu

دور الارادة في المسؤولية الجزائية == The Role of Will In Penal Resposibility

Author name: معاذ جاسم محمد العسافي
Supervisor name: جمال ابراهيم عبد الحسين الحيدري
General topic: Law
Specific topic: Criminal Law
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: This study tackled the role of will in penal responsibility. The reason behind tackling this subject were introduced in the introduction, especially that will is considered one of the important subjects in the general theory of sanctions law for it is as

المسور بن مخرمة دراسة في مروياته التاريخية == Almuswr Bin Makhrama Astudy Of His Historical Narratives

Author name: سجاد حنتوش شوكان الزيادي
Supervisor name: عباس جبير سلطان التميمي
General topic: History
Specific topic: Islamic History
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:
Abstract: Our study tackled the historical narratives only about the personality of Al Mswer Bin Mekhremeh, that we searched about the oldest available references. He was one of the minor prophet's(p.b.u.h.) companion. He lived in the prophet's time. He had a role during the orthodox caliphs, especially in the caliph's Omar Bin Al Khettab time. He had also an important role during the time that followed killing the caliph Omar when he participated with his uncle Abdul Rehman Bin Ouf in choosing the third caliph Othman Bin Iffan when he was the right hand to his uncle to choose the caliph in spite of the signs that he had a tendency towards the prophet's family. He had good stand with Al Imam Ali, Al Imam Al Hassan ,and Al Imam Al Hussein. After choosing Othman as caliph, they depended on him in many issues like sending him as an envoy to Africa and other states. The caliph Othman also relied on him when he sent to MuawehBin AbiSufyan besieging him to raise the blockade when he was surrounded by the angry rebels in the house accident. But Muawehdid not respond to his request, the matter that made the caliph angry on Muaweh. Al Mswer also had an opposite opinion to the Umayyad, but Muawehby his slyness could attract Al Mswer and made him calm. His opinion towards Umayyad remained him clear. That is after Muaweh'sdeath his stand was against the Umayyad when Yazid was chosen as caliph when he stood beside the Zubairis in the war against the Umayyad where he was killed by a canon stone that was thrown towards the hornableKabba which wasbesieged by the Umayyad and he was buried there. What concerns our study, it consisted of three chapters, an introduction, and a conclusion. In the introduction, reasons behind choosing the topic were studied as well as the difficulties that have been faced. The first chapter talks about Al Mswer's social, cultural, and political life. Several issues about Al Mswer's antecedents, fathers, and birth ambiguities ending with his death. Concerning his cultural activity, he was considered one of the legists where he had speeches about the prophet. The political aspects and stands in his life were discussed, particularly the last about Al Zubairi and hid death. The second chapter was about this historical narratives that were before and after prophecy. Some of these were about events that happened during the prophet's time like wars led by the prophet. Some of them belong to Al Mswer's personal issues. Some of the narratives were deeds to Al Mswer which were close to the prophet. The third chapter was about Al Mswer's narratives during the orthodox caliphs and part ofMuaweh's time. In this chapter we found his clear activity during the caliph Omar Bin Al Khettab and his witness to the events at that time such as Al Remadah Year and later killing of the caliph Omar and the election issue as well as his role. Later some narratives during the caliph Othman Bin Iffan and his uncle's Abdul Rehman Bin Ouf deeds were mentioned. After that we see turning his narratives about Othman's mistakes when he gave fortune to his relatives rather than other people. That what made people rebel and kill Othman. He had one or two narratives duringMuaweh Bib AbiSufyan. At last Al Mswer's narratives were studied concerning the source and narrated people as well as studying the narrative body to select the perfect
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منهج المروزي وموارده في كتاب طبائع الحيوان : مقالات منتخبة من المقالة الاولى باب الصين والترك والهند == Al - Marowzi Approach And Its Resources In The Book Of Tba'E Alhaywan Selected From The First Article Chapters Of China, Turk And India

Author name: غازي هادي حمزة اليساري
Supervisor name: هاشم ناصر حسين الكعبي
General topic: History
Specific topic: Islamic History
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:
Abstract: هذه الاطروحة تقدم المؤرخ والجغرافي والطبيب شرف الزمان طاهر المروزي - كان حيا ( سنة 518هـ/ 1134م ) - وبعنوان ( منهجية المروزي في كتاب ابواب الصين والترك والهند , ابواب منتخبة من كتابه طبائع الحيوان وموارده ) , وهذه الدراسة تاتي ضمن حقل الدراسات التاريخية و| This thesis of progress and geographic and doctor sharaf al - zaman tahir al - Marowzi - was alive (year 158 AH \ 1134 AD) - And entitled (Al - Marowzi methodology in the book of the Chapters of China and Turk and India , Elected chapters of his book Tba'e Alhaywan and resources) , This study is part of the historical and geographical studies field That are interested detects historians Arab Muslims efforts , And to clarify their approach to authoring and resources and their tactics in dealing with the events witnessed by the Arab Islamic nation , And the date of their neighboring nations and clans , Hence the desire to record the subject came , and the approach of Al - Marowzi was in the writing of the three chapters - China and Turk and India - particularly strong motivation towards writing this thesis. Sharaf al - zaman tahir al - Marowzi considered is one of the leading historians of Arab Muslims who wrote about the Levant and Morocco typing known Tba'e Alhaywan - The first article of it - And the known world of his time , The polls and note down what is received from this historic Nations resources , Especially the subject of our study - The country of China, India and Turk - and He writes what is being offered to him and raises his attention , And the notices of the customs and traditions and systems and civilization of the different nations and peoples in this country that He lived with al - Marowzi.The Research Plan Research divided into preliminary and introduction, four chapters and a conclusion , The first chapter came in three sections , The first section took care of study of the life and geographic and doctor sharaf al - zaman tahir al - Marowzi , Such as his name and lineage scientific and upbringing and of his travels in the Arab Islamic countries , The second section we are believe in The influence of the age on the person of the author , So I dealt with the most prominent features of his time in the Seljuk state and its capital city of Mero, where he lived al - Marowzi , What characterized this era of conflict between the political forces that tried to seize power and the conduct of scientific movement , On the contrary, this contributed to the competition between scientific schools in multiple centers , The most important schools Baghdad, Cairo, Cordoba and Maro , In this study we have shown the flourishing of science and medicine in the Seljuk era. Then came the third section , Where we discussed the Arab - Islamic relations with the countries of India and China until the fall of the city of Baghdad by the Mongols in the year 656 AH , 1258 AD , Bypassing the country of Turk , To the fact that this relationship has come under the second section, which we introduced the first Seljuk state and growing up in the country's Turk and its extension after that to the other country,And its domination on the territory of the Abbasid state in the fifth century AH, the eleventh century AD In the second chapter , I thought that we should deal with the first section and briefly summarize the development of historical writing among Arab Muslims , And its early stages and its growth and development in the successive Arab and Islamic Arab countries until the era of al - Marowzi , And the stages in which it passed , From the style of biography and news , To the style of human being and the idea of the nation , Then we presented a second section on the geography of the Arabs , I presented the possession of the Arabs before to Islam geographical information astronomical benefited from them in their travels and movements , Then we showed the interest of Muslim Arabs in geographical knowledge, trips and trends , and The development of Arab - Islamic geographical authorship, from the keen attention of the Islamic regions to specialization in one country , And then the tendency to classify the geographical dictionaries influenced by the geography of other neighboring nations, especially the Greeks and Romans after the translation of the works of these countries , And in the third section dealt with the talk about the development of the emergence of animal science among Arab Muslims , Before the spread of the Islamic religion in the Arabian Peninsula and the development of this science during the successive Arab Islamic times , And the stages that passed until the era of Sharaf al - zaman taher Al - Marowzi and classification of the book of Tba'e Alhaywan. After learning about the development of the history, geography and animal sciences of Muslim Arabs, I had to learn in the third chapter the approach of Doctor Sharaf Al - Zaman Taher Al - Marowzi in writing the chapters of China, Turkey and India in his book Tba'e Alhaywan , And divided his sections into three paragraphs , presented in the first paragraph , The definition of the book , As it touched on the control of the title and proportion and date of composition , And the purpose of writing , Manuscripts , Typography , Arrangement and division and divided the investigation into three paragraphs , In the second paragraph, the basics of the organization and presentation of the historical article were presented in the three sections according to the sections , And the most important texts of rare historical documents presented by Al - Marowzi and unique in the chapter of China , In the third paragraph of this chapter, Al - Marowzi's method and methodology were presented in writing the three chapters and quotations from the ancient Greek heritage and Arab Islamic sources , Resources and elders , In addition to the style of criticism of the novels and the beginning of transport and end and the flags mentioned, all with live examples and resources Al - Marowzi within the three chapters - the study research. And to meet the title of this treatise right , I devoted the fourth and last chapter to talk about the cultural aspects in the countries of China, Turkey and India and was extrapolated from the total resources of Al - Marowzi in the three chapters , In which there are clear indications about the geographical aspects in the countries of China and Turkey and India and its locations and seas and cities and borders and how to manage this country and that in the first section , The second section has included Al - Marowzi resources for religious values and principles of ideological and social and cultural manifestations in the country of China, India and Turk , Finally, the third and final section was devoted to the economic aspects of this country , The research then concluded after that with a conclusion summarizing the research and its results , This was followed by a detailed outline of the basic sources and secondary references from which they were reported.Study of sources It is obvious to those who study the subject as the subject of this thesis , The researcher should not expect to find his scientific article in a wide range of ancient primary sources , because This type of studies and research , Have been shown only at very close intervals in comparison to other studies and research , Hence, the authoritative source on which this study is based is Al - Marowzi's own book (Tba'e Alhaywan) , And is the real source and solid foundation on which this study was built , It is from this book that information was extracted and conclusions drawn , And devising ideas , And building judgments and observations , Therefore, the reader will notice the frequency of the name of the book and its articles and chapters frequently , During the chapters and sections of this study
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