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Effect Of Some Additives On Fire Retardance And Bending Strength Of Unsaturated Polyester (Upe) Composite

اسم المؤلف: محمد هلول مخيلف الشمري
الموضوع العام: علوم الفيزياء
السنة: 2006
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

تحضير وتشخيص ليكاند قاعدة شف جديدة مشتقة من 4 - امينو انتي بايرين ومعقداتها مع بعض الايونات الفلزية ودراسة فعاليتها المضادة للبكتيريا Synthesis And Characterization of New Schiff Base Ligand Derived From 4 - Aminoantipyrine And It'S Complexes With Some Metal Ions And Their Antibacterial Effective Study

اسم المؤلف: هيام هادي علكم
اسم المشرف: ساجد محمود لطيف
الموضوع العام: علوم الكيمياء
السنة: 2013
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمن البحث تحضير ليكاند قاعدة شف جديدة (L) ثنائية السن نوع (NO)1,5 - dimethyl - 4 - ( 2 - oxo - 1,2 - diphenyl ethylidene amino) - 2 - phenyl - 1,2dihydropyrazol - 3 - one.وذلك من مفاعلة 4 - aminoantipyriene مع Benzil باستعمال الايثانول وسطا للتفاعل. | This work covers the synthesis and characterization of the new Schiff base bidentate ligand(L) type (NO) 1,5 - dimethyl - 4 - (2 - oxo - 1,2 - diphenylethylidene amino) - 2 - phenyl - 1,2 dihydropyrazol - 3 - oneThe ligand (L) was prepared from reaction of 4 - aminoantipyrine with Benzil under reflux in ethanol solvent. The Ligand Complexes for the metal ions : VO(II) ,Mn(II) , Co(II) , Ni(II) , Cu(II) , Zn(II) , Cd(II) and Hg(II) were prepared. The Ligand and its complexes were characterized by spectral methods [FT - IR , UV - Vis , Atomicabsorption , 1HNMR ] in addition to molar conductivity , Microanalysis (C.H.N) , Magnetic moment effect and melting point and mole ratio Ligand : Metal. From the above data for the ligand and its complexes , we suggested that the ligand (L) behaves as bidentate on complexation with metal ions Via Natom of ( C = N) group and O atom for ( C = O) of pyrazol ring. The suggested molecular formula and geometrical structure are : 1 - Squarepyrimide for [VO(L) (SO4) (H2O) ] 2 - Tetrahedral for [M(L)Cl2]. n H2O M = Zn (II) , n = 1 M = Cd(II) and Hg(II) ; n =0 3 - Octahedral for [M(L)2Cl X ]. Y M = Mn(II) , X = Cl , Y = H2O M = Co(II) , Ni(II) and Cu(II) X = H2O , Y = Cl

تحضير وتشخيص بعض المعقدات الفلزية احادية ومختلطة الليكاند المشتقة من سلفاميثاكزول و4,4' - ثنائي مثيل - 2,2' - باي بريدال Synthesis And Characterization of Some Metal Complexes With Mono And Mixed Ligand Derived From Sulfamethoxazole And 4,4' - Dimethyl - 2,2' - Bipyridyl

اسم المؤلف: ميسون مزهر عـبد الحسـن
اسم المشرف: محاسن فيصل الياس
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء اللاعضوية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم في هذا البحث تحضير معقدات جديدة للعناصر الثقيلة Rh(III)} وPd(II) Pt(IV)و {Au(III) من تفاعل الليكاند (sulfamethoxazole L1) مع ايونات املاح هذه الفلزات بالطريقة التقليدية وتم تحضير معقدات جديدة اخرى في الحالة الصلبة بواسطة تفاعل مزيج من (sulfamethoxazol | In the present study, new heavy metal complexes of sulfamethoxazole (SMX) as L1 have been prepared with Pd(II), Au(III), Rh(III) and Pt(IV) ions , in a solid state by conventional method. Two mixed ligands were chosen : sulfamethoxazole and 4,4/ - dimethyl - 2,2/ - bipyridyl (L2). This is done to prepare another series of complexes with some metal ions ( Co(II), Ni(II), Cu(II), Cd(II), Cr(III) , V(IV), Pd(II) and Au(III)) in order to investigate the coordination behavior of these ligands(L1) and (L2) toward these metal ions.These complexes, already prepared by the solid state, were characterized by the elemental analysis (C.H.N.S) and FT - IR , UV - Vis spectroscopy, in addition to the flame atomic absorption, magnetic susceptibility , melting point and conductivity measurements. According to the results obtained, it is noticed that ligand L1, with the light and heavy metal ions, clearly behaves as a bidentate through the O atom of sulfonyl group and N atom of sulfonylamid group for all the prepared complexes except Cu(II) and Ni(II). In this case, the ligand confirms that the bonding of the metal ion in a tridentate chelate through the O atom of sulfonyl group and N atom of sulfonylamid group and N atom of amine group.While L2 behaves as a bidentate ligand through two N atom. Conductivity measurements have shown that all the prepared complexes are ionic except PdL1L2 complex. Based on the results of the measurements, the following formula have been suggested for the new prepared complexes : - [VOL1L2]SO4.2H2O and [CuL1L2]2( NO3)4.0.5H2O have square pyramidal geometry while the following complexes are having octahedral geometry : - [CrL1L2 Cl2] Cl.H2O,[NiL1L2NO3]2 ( NO3)2.0.5 H2O,[PdL1L2 Cl2].0.5H2O,[PtL1Cl3H2O]Cl.H2O, [Au L1L2Cl2] Cl.2H2Oand the complexes below have tetrahedral geometry : - [CoL1L2] (NO3)2.3H2O,[CdL1L2](NO3)2.3H20 while [PdL1Cl]2 Cl2.H2O,[AuL1Cl2]Cl.3.5H2O,[RhL1ClH2O]Cl2.0.5H2O have square planar geometry.Different bonding and structural behaviors were revealed throughout the study of coordination chemistry of the newly prepared metal complexes.The nature of bonding between the metal ion and the donor atoms of the ligands were demonstrated by calculating Racah parameter and the other ligand field parameters which were calculated using suitable Tanaba - Sugano diagrams.The nature of the complexes in ethanol solution was studied for some of the prepared complexes such as (RhL1,PdL1,PtL1 and AuL1) in the solution state to determine the ratio between ligand to metal state by using the molar ratio method which gave results which approximately identical results when compared to those obtained from the isolated solid state. Besides the stability constant of the prepared complexes were studied and it was found that they were stable in molar ratio 1 : 1.

تحضير وتشخيص مشتقات جديدة للكومارين - 3 - كاربوكسلك اسيد Synthesis And Characterization of New Derived From Coumarin - 3 - Carboxylicacid

اسم المؤلف: كوثر عبد الواحد عبد الحميد
اسم المشرف: شذى فاضل نارين الزبيدي
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء العضوية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعد مشتقات الكومارين مهمه من الناحية البايلوجية والصناعيه والدوائيه وعلى هذا الاساس استمرت الدراسات في تحضيرمشتقات جديده للكومارين وعليه فقد تم في هذا البحث تحضير عدد من مشتقات للكومارين وعددها 23)) بالاضاقة الى المركب 2 - oxo - 2H - coumarin - 3 - carbox | The coumarin and his derivatives class of heterocyclic compoundsThey are presente in a large family of products with broad biological and industrial activities.This fact leads as to synthesis twenty eight compounds of heterocyclic by several methods show in diagrams (1, 2).Than we characterized this compounds by some spectral analysis Such as : (FT - IR), (GCMS), (H - NMR) and determination of their physical properties such as melting points and color.This work involved : 1. Synthesis of base compound ( coumarin 3 - carboxylic ) by reaction Malonic acid with Salicylaldehyde in Petroleum ether as a solvent.2. Synthesis of k1 by reaction ( coumarin 3 - carboxylic ) with Thionyl chloride in Dichloro methane as a solvent.3. Synthesis of k2 by reaction k1 with hydrazine hydrate 99%. 4. Reaction k2 with six kinds of aromatic aldehyde to Synthesis of Schiff Bases : K8 - k7 - k6 - k5 - k4 - k35. Synthesis of five from Tetrazoles compounds by reaction Schiff Bases With sodium azide in dioxane as a solvent. 6. Synthesis of five from thiazolidinone compounds by reaction Schiff Bases with Mercapto acetic acid in THF) as a solvent. 7. Synthesis of five from azetidinone compounds by reaction Schiff Bases with ChloroacetylChloride in Dioxane as a solvent. 8. Synthesis of five of thiourea compounds by reaction Schiff Bases with thiourea in methanol as a solvent.

دراسة دورالانترلوكين - 36 كاما والبارااوكزونيز وحامض السياليك ومتغيرات اخرى في مصل المريضات بهجرة بطانة الرحم في بغداد Study The Role of Interleukin - 36? , Paraoxonase , Sialic Acid And Other Parameters In Sera of Endometriotic Patients In Baghdad

اسم المؤلف: رشا زهير جاسم
اسم المشرف: زهير ابراهيم المشهداني بشرى حميد علي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الحياتية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Endometriosis , a chronic inflammatory autoimmune disease, is among one of the most challenging of the 21st century that affects women in reproductive age. Seventy five consecutive married women endometriotic patients with age range (25 - 40) year were enrolled in this study , divided into three groups , the first included twenty five newly diagnosed endometriotic patients ( without any treatment) , the second consisted of twenty five endometriotic patients who were treated with zoladex for 3 to 5 months , the third involved twenty five patients with recurrent endometriosis (post treatment of zoladex) for one to two years ago. Patients groups were compared with two matched age and sex control groups , control group included twenty five healthy women while pathological control group involved twenty five women suffering from infertility caused by gynecological disorders not linked with endometriosis. The present study highlights the role of some morphological characteristics (BMI and W/H) , immunological / inflammatory aspect (Interleukin - 36 ? , Angiopoietein - 2 , Tumor Necrosis Factor - ? , Immunoglobulin G , Immunoglobulin A , Immunoglobulin M , Sialic acid ) , oxidative aspect (Total Cholesterol , Triacylglycerol , High Density Lipoprotein cholesterol , Low Density Lipoprotein cholesterol , Very Low Density Lipoprotein cholesterol , Paraoxonase - 1 , Ceruloplasmin ) and hormonal aspect (estrogen) in the pathogenesis of endometriosis. …………… Results have revealed that endometriotic patients have approximately a normal BMI and lower than pathological control group, but it was increased in patients under treatment with zoladex. A greater W/H ratio was associated with both endometriosis and pathological control group , while W/H was approximately not affected after treatment with zoladex. The present study have reported for the first time a positive relationship between IL - 36? , Sia and endometriosis. Those parameters could be considered novel biochemical markers in endometriosis because their levels were higher in sera of endometriotic patients without treatment compared with control and pathological control groups. Moreover , Ang - 2 appears to be a good biochemical marker in endometriotic patients. Hence, Ang - 2 levels were higher in sera of endometriotic patients compared with control and pathological control groups In contrast high levels of TNF - ? are associated not only with endometriosis but with pathological control group also. IgA and IgM could be used as possible biochemical markers for endometriosis. The present study is the first dealing with zoladex action on depressing IL - 36? , Ang - 2 , Sia while TNF - ?, IgG , IgA, IgM were decreased under treatment with zoladex. The present study have also suggested that TC and LDL - could be used in diagnosis of endometriosis , HDL - c levels were lower in patients without treatment compared with control and pathological control, but elevation obsereved under treatment with zoladex. PON - 1 activity was low in sera of patients while it was increased after treatment. Furthermore , this is the second study that proves a positive relation between CP and endometriosis and the first dealing with zoladex role on decreasing CP level in endometriosis. Lastly , estrogen was higher in endometriosis compared with control and pathological control groups , while it was decreased by zoladex action

تحضير ودراسات طيفيه لمعقدات ليكاندات الاميدوبنزوثايزول الممزوجه باستخدام الطريقه المايكرويفية Synthesis And Spectral Studies of Amide Benzothiazole Mixed Ligands Complexes Using Microwave Method

اسم المؤلف: فرح سعدون جعفر
اسم المشرف: محاسن فيصل الياس
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء اللاعضوية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: في هذا البحث تم استخدام احدى تطبيقات الكيمياء الخضراء حيث حضرت المعقدات بالطريقة المايكرويفيه حيث تتيح هذه الطريقه ظروف افضل للعمل من حيث تقليل التلوث ,استخدام كمية مذيب اقل, اقل تكلفة, نسبة منتوج عاليه , تفاعل بزمن اقصر وكطريقة عمل ابسط.تم استخدام ليكا | One of green chemistry applications has been used in this work ,where the complexes were prepared by microwave irradiated reactions that availed reduced pollution, free or less solvent conditions, low cost, high yields, shorter reaction times and simplicity in processing. Two of benzothiazole derivatives ligands 2 - benzamid benzothiazole (L1) and 2 - acetamid benzothiazole (L2) were used to synthesize two types of transition metal complexes ; Rh(III), Pd(II), Cd(II), Pt(IV) and Au(III) , the other set of complexes was prepared in presence of a co - ligand 1,10 - phenanthroline(L') or 4,4? - dimethyl - 2,2? - bipyridyl (L") with the metal ions V(IV), Cr(III), Co(II), Ni(II), Cu(II), Zn(II) and Au(III) using microwave and conventional methods to compare the results between them. These ligands and their metal complexes were isolated and characterized in solid state using FT - IR, UV - Vis spectroscopy, flame atomic absorption, elemental analysis C.H.N.S. and magnetic susceptibility measurements as well as melting point and conductivity measurements. According to the results of the above measurements, the following shapes were suggested for the prepared complexes : Complexes of ligand (L1) : Complexes of Rh(III), Pd(II) and Au(III) have a square planar geometry, while Cd(II) complex has a Td geometry and Pt(IV) complex has an Oh geometry.Complexes of mixed ligands (L2 with L'/ L") : All complexes have an Oh geometry except V(IV) complex has a square pyramid and Co(II) complex has a Td geometry. The nature of bonding between the metal ion and the donor atoms of the ligands was demonstrated through the calculation of Racah parameter and other ligand field parameters, which were calculated using suitable Tanabe - Sugano diagrams.The nature of some (L1) complexes in liquid state was studied by following the molar ratio method which gave results approximately identical compared with those obtained from the isolated in the solid state; also, stability constants of the prepared complexes were studied , they were stable in the molar ratio 1 : 1. A theoretical treatment of the ligands and the prepared complexes in gas phase was done using two programs; Hyperchem - 8 and Gaussian program (GaussView Currently Available Versions (5.0.9) along with Gaussian 09 which is the latest in the Gaussian series of programs).Hyper chem. - 8 program used the molecular mechanics and semi - empirical calculation, the heat of formation (?H?f), binding energy (?Eb) dipole moment (µ) for the free ligands and their metal complexes were calculated using ZINDO/1, PM3 and AMBER methods at 298 K. It was found that the complexes were more stable than their ligands; furthermore, the electrostatic potential of free ligands was calculated to investigate the reactive site of the molecules, PM3 was used to evaluate the vibrational spectra of free ligands ,the obtained frequencies agreed well with those values experimentally found; in addition, the calculation helped to assign unambiguously the most diagnostic bands. Electronic spectra measurements for the ligands were calculated theoretically using ZINDO/S method comparing it with the experimental results. It was found that there was a closely relationship between the theory and experimental spectra.Gaussian program semi - empirical (PM3) method which used in order to calculate : the geometry optimization, dipole moment (?),total energy ,electrostatic potential, ELUMO and EHOMO was obtained, evaluate the vibrational spectra of free ligands and these obtained frequencies agreed well with those values experimentally found.Also electronic spectra measurements for the ligands were calculated theoretically by using the job type : Single point energy (SP) along with ZINDO method and also the job type frequency (Freq) used along with CIS method (3 - 21G).

تقدير الخصائص الفيزيائية والكيميائية لمياه الابار المحلية بوساطة تقنيات تحليلية مختلفة لتحديد نوعيتها وصلاحيتها للاستخدام البشري والزراعي Determination of Physical And Chemical Characteristics of Local Wells Water By Using Different Analytical Techniques For The Establishing Their Quality Specifications And Usage Conformity

اسم المؤلف: احسان نصيف جاسم العبيدي
اسم المشرف: كريم ديمة خلف كاميران حسين شكر
الموضوع العام: علوم الكيمياء
السنة: 2008
الموضوع الدقيق: الكيمياء التحليلية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: مؤخرا، ازدادت ظاهرة حفر الابار في المنازل والمناطق المحلية خصوصا بعد الحرب على العراق عام 2003 تبعا لشحة تجهيز الماء الصالح للشرب من قبل السلطات المسؤولة. هدف البحث اساسا لتعرف نوعية مياه الابار المحلية ومدى صلاحيتها للاستهلاك البشري والمنزلي، با | Recently , the phenomenon of drilling wells in the local areas and housings has increased before the war on Iraq 2003 due to the anticipations of insufficient supply of drinking water by the competent authorities. This research work is aimed to recognize the quality of the local wells water quality and the extent of their adequacy for human and domestic consumption. In addition to try to know the relevance of these wells water and the nearest point of surface water (i.e. river water). In this study , five wells were selected in Al - Adheem Embankment area and the surface water of Al - Adheem River itself. The sampling strategy of the two type of water began in 1/3/2007 and the process of sampling is complied with guidelines cited by national and international specifications , This piece of study involves four chapters. Chapter one deals with chemical literature review concerning environment and pollution , sources of groundwater pollution in addition to the physical and chemical characteristics of water such as color turbidity , electrical conductivity , TDS , TSS , pH , total hardness, Ca2+ , Mg2+ , Cl - , SO42 - , NO3 - , NO2 - and heavy metals contaminates (Cu2+ , Cd2+ , Ni2+ , pb2+ , Zn2+). Chapter two describes all methodology and techniques used in this study such as atomic absorption spectrometry (both FAAS and ETAAS) , UV - Vis spectrophotometry , pH - meter , conductivity meter , and sampling protocol according to those cited by national and international bodies. In addition to , the all procedures for measuring the constituents and characteristics of water samples were described. In chapter three , the outcome of this research work were reviewed and discussed for each constituent as mentioned above. The results of study are summarized in the table hereafter : Chapter four explicates the conclusions that attained by this research work showing the important study accomplished for the first time in this area of Iraq in one hand. In the other hand , it showed that some characteristics agree and disagree with the specifications of drinking water that are listed by Iraqi and other international bodies. Finally , It concluded that all water recourse selected in this work were not drinkable and improper for human consumption according to national and international specifications , but it could be useful for arable particularly for that plants stand high concentrations of salts.

تطوير طرائق طيفية لتقدير السلفاميثوكسازول والسلفاديازين باستخدام تفاعل الاقتران الازوتي Development of Spectrophotometric Method For The Determination of Sulphamethoxazole And Sulphadiazine Using Diazotization Coupling

اسم المؤلف: امل حسين محيميد حسين
اسم المشرف: سعدية احمد ظاهر
الموضوع العام: علوم الكيمياء
السنة: 2011
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اشتملت هذه الرسالة على ثلاثة فصول : يتضمن الفصل الاول استعراضا موجزا لاملاح الديازونيوم والكاشف الثايمول واهمية ادوية السلفا فضلا عن بعض الطرائق الحديثة في تقدير ادوية السلفا والهدف من اجراء البحث.واشتمل الفصل الثاني على وصف طريقة طيفية بسيطة وحساسة | his thesis consists of three chapters : - the first chapter includes a short notes on the diazonium salt and thymol reagent demonstrates a brief review for, an assay of sulpha drugs and aim of the work.The second chapter describe a simple sensitive spectrophotometric method for the determination of microgram amounts of sulphamethoxazole in aqueous solution, based on the coupling of diazotized sulphamethoxazole with thymol reagent in alkaline medium to produce an intense yellow coloured ,water - soluble and stable azo - dye which exhibits maximum absorption at 473 nm. The determination limits of Beer’s law were 1 - 6 µg.ml - 1, with a molar absorptivity 2.1 × 104 l.mol - 1.cm - 1. The average recovery was 100.12 %, and RSD 0.492 %, the LOD is 0.0087 ?g.ml - 1 and LOQ is 0.029 ?g.ml - 1. The method has been successfully applied for the determination of sulphamethoxazole in pharmaceutical preparations where the analytical results are compatible with certified value of pharmaceutical preparations and with a standard addition procedure.The third chapter demonstrates the development of simple sensitive spectrophotometric method for the determination of microgram amounts 1 - 7 µg.ml - 1 of sulphadiazine , based on the formation of diazotized sulphadiazine by adding sodium nitrite in acidic medium; followed by removing the excess of nitrite by sulphamic acid, the formed diazotized sulphadiazine was then coupled with thymol in strong alkaline medium to get a yellow coloured azo dye. The produced dye is stable and soluble in aqueous medium and it has maximum absorption at a wave length of 469 nm. The molar absorptivity was 2.6 × 104 l.mol - 1.cm - 1 with LOD and LOQ 0.0077 and 0.025µg.ml - 1 respectively. The method had good accuracy and precision; The average recovery was 100.57 % and RSD 0.657 %. The method was applied on the pharmaceutical preparation of sulphadiazine (as cream), the analytical results were in agreement with certified value of pharmaceutical preparation and with a standard addition procedure.

تحضير وتشخيص ليكاندات ازو جديدة ومعقداتها ودراسة تطبيقاتها الصناعية والبكتيرية Synthesis And Characterization of New Azo Ligands And Their Complexes And Studying Their Industrial And Bacteriological Application

اسم المؤلف: زينب سلمان كاظم
اسم المشرف: عامر جبار جراد
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تتضمن هذه الدراسة تحضير ثلاثة ليكاندات ازو جديدة (L3 - L1) ارتكزت جميعها على 2،4 - ثنائي مثيل فينول، تمتلك الصيغ التركيبية الاتية : - شخصت الليكاندات المحضرة باستخدام (FTIR, UV - Vis, 1HNMR) والتحليل الكمي الدقيق للعناصرC.H.N) )، فضلا عن درجات الانصهار. | Three new azo compounds (ligands) L1 - L3 have been prepared all rest on the 2,4 - dimethylphenol as a back - bone of the prepared ligands having the following structures.The prepared ligands were characterized by melting points measurements, IR, 1HNMR and UV - Vis spectra and (C.H.N) analysis. The other part of this project refer to the synthesis of complexes of the ions Ni(II) and Cu(II) with all prepared ligands.All preparation was performed after fixing the ideal pH and molar concentration that obeyed Lambert - Beer’s law in the studied pH ranges. The structure of these complexes was deduced according to the molar ratio and Job methods depending on the spectroscopic studies of the complex solution of the above ions. However, ration of 1 : 2 M : L for all ions were obtained.The prepared complexes were characterized using IR and Uv - Vis spectra conductivity magnetic susceptibility and melting points measurements. The percentage of the metals in the complexes has been found by flameless atomic absorption technique. Micro elemental analysis (C.H.N.) were also measured, the results are in agreement with the calculated values. All the complexes are quite stable and could be stored for months without any appreciable change. According to the results obtained by elemental and spectral analysis, an octahedral structure was suggested for the prepared complexes with the ligand (L1) and mixed ligands (L1,L3)while tetrahedral for the all prepared complexes with the ligand (L2) and ligand (L3).In addition the dyeing performance of the prepared compounds was assessed. The dyes were tested for light and detergent fastness. Study of biological activities of the prepared compounds has also been performed. The study was carried out using Escherichia Coli, Staphylococcus aurous and Bacillus cereus in agar medium. Some of the complexes exhibit good bacterial activities.

تحضير وتشخيص انواع جديدة من الاطيان الهجينة بطريقة ادخال ايونات المعادن باستخدام اطيان السمكتايت العراقية Preparation And Characterization of Anew Hybrid Clays By Intercalaion With Metal Ions Using Iraqi - Smectite Clays

اسم المؤلف: فرات لطيف يحيى شريف
اسم المشرف: محمد حسن عبد اللطيف
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The aim of this study is to prepare two types of hybrid Iraqi Clays, which have been magnetic Properties by using the mothed of Intercalation.In this study, we have used two types of Iraqi clays (Bentonite and Attapulgite) with a saturated solution of ferrous chloride aqueous (FeCl2.4H2O) , ferric chloride aqueous (FeCl3.6H2O) and Super Magnet solution (Nd2 Fe14 B), the clays activate firstly by using the way of the ionic exchange with industrial Ionic exchanger on the formula (H - Form) , then we intercalate different percentage of iron ions and Super Magnet to that clays.They have used different analytical techniques in this study which include : Magnetic Susceptibility ,Infrared spectroscopy , X - ray diffraction spectrum and Scanning Electron microscopy. the results were have obtained as follows : 1 - The activation process has led to removal the impurities and thus led to increase of the proportion each of Montmorillonite and Attapulgite metal.2 - Magnetic Susceptibility of Initiated Clays are higher than the Magnetic Susceptibility of crude clays.3 - Magnetic Susceptibility increases due to intercalate two types of iron ions, as well as intercalated Super Magnet and has models depended in Table (3 - 2) as the higher models of Magnetic Susceptibility with bentonite clay and models in Table (3 - 13) are higher models of Magnetic Susceptibility with Attapulgite clay, 'the model number (1) is the highest Magnetic Susceptibility models of Super Magnet with clay bentonite, either Attapulgite clay has increased Magnetic Susceptibility by using all ratios in Table (3 - 27).4 - Infrared spectrum has showed appearance of clear peaks back to Hematite and Magnetite which have two types of iron oxides, which own magnetic properties and the peaks have notpresented in the spectrum of the raw clay for each of bentonite and Attapulgite as well as appearance of new peak back to hydroxide neodymium at intercalated the Super Magnet.5 - Powder X - ray diffraction spectrum has Showed an increasing in the intensity of each of the Montmorillonite and Attapulgite metal after activation process' After intercalation process we note a decrease in the intensity of Montmorillonite and Attapulgite metal because of the entry of different ratios of iron ions As well as (Nd2 Fe14 B) This is evidence formation of hybrid clays where the X - ray spectrum showed appearance of new peaks back to Hematite , Magnetite and Super Magnet, as well as an increase in the intensity peaks of Hematite in the original clay.6 - Scanning Electron microscopy image has shown on a clear change in the crystalline structures that have appeared on the surface of clay which show the success of intercalation process.Through the results we have obtained through this study that the method used in Activation of clay and intercalation of two type of iron as well as a Super Magnet is a successful method and can be depended in the preparation of Clays possess magnetic qualities can be used in high - efficiency adsorption and ion exchange applications.

تخليق قواعد شف جديدة مشتقة من انهدريد البايرومليتك Synthesis ,Antibacterial of Some Novel Schiff Bases Derived From Pyromellitic Dianhydride

اسم المؤلف: عذراء محمد سلوم
اسم المشرف: امينة عبد الرحمن فياض عماد تقي علي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم تخليق قواعد جديده مشتقة من انهدريد ثنائي البايرومليتك بعدة خطوات. حيث حضرت مشتقات حامض البايرومليتك المتناظره a­c[ I ] من تفاعل انهدريد ثنائي البايرومليتك مع بعض الامينا ت الاورماتية الحاويه على (مجاميع ساحبه واخرى دافعة ) في الاسيتون كمذيب. واشتملت | The novel Schiff bases derived from pyromellitic dianhydride were synthesized by several steps reaction. A symmetrical diamic acide [I ]a - c was synthesized by the reaction of pyromellitic dianhydride with some aromatic amines (containing electron withdrawing , repulling groups ) in dry acetone.In the second step the diacids were converted to their corresponding diimide [II ]a - c using acetic anhydride and sodium acetate system as a dehydrating agent, via the intramolecular cyclization steps of amic acids.The third step was to synthesis the hydrazone derivatives from the reaction of diimides , with 80 % hydrazine hydrate at (50 - 60)C?.These hydrazone derivatives were allowed to react with several aromatic aldehydes to form new Schiff bases via step four at a temperature near by (80 - 85)C?. As shown in the following scheme (3.1). All the novel compounds and their structures have been ascertained by their melting points , mixed melting points , C.H. N analysis , FTIR , UV - Viss. and 1HNMR spectroscopy for some of them. Also the biological activity of some of them was studied which showed that these compounds have different activities towards the studied bacteria.

تحضير ودراسة طيفية وامتزاز لمعقدات بعض ايونات العناصر الانتقالية لليكندات المشتقة Synthesis Spectroscopic And Adsorption Studies of Some Ions Transition Metal Complexes For Ligands Derived From Sulfamethoxazole And Its Mixed Ligands

اسم المؤلف: فرح علي داود
اسم المشرف: ساهرة صادق عبد الرزاق احمد ثابت نعمان
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان الهدف من الدراسة هو تحضير وتشخيص ليكاند ثنائي السن : - [4 - (5,5 - dimethyl - 3 - oxocyclohex - 1 - enylamino) - N - (5 - methylisoxazol - 3 - yl)benzene sulfonamide]من تفاعل السلفاميثوكسازول مع الدايميدون لينتج الليكاند [H2L] باستخدام طريقة الصهر ا | The aim of the study is the preparation and characterization of bidentate Ligand [4 - (5,5 - dimethyl - 3 - oxocyclohex - 1 - enylamino) - N - (5 - methylisoxazol - 3 - yl) benzene sulfonamide]. The ligand was prepared by fusing sulfamethoxazole and dimedone at (170) ?C for half hour. The prepared ligand was characterized by FTIR,UV - Vis spectroscopy, H1,C3 - NMR spectra, mass spectra, molar conductivity measurement and melting point. The molecular structure for the ligand [H2L] which was proposed as drawn in the figure below : - Ligand [H2L]The complexes prepared using reflux in ethanol as solvent were two types namely : - 1 - Complexes of (1 : 1) mole ratio formula [M(H2L)(H2O)2]Cl22 - Complexes of (1 : 1 : 1) mole ratio formula [M(H2L)(HA)] in the presence of KOH as a base, Where : - M(II) = (Co ,Ni ,Cu) HA= 3 - amino phenol. Spectroscopic methods (FT - IR, UV - Vis spectroscopy) along with (A.A), chloride content and melting point were carried out in addition to molar conductance were done for the characterization of all complexes.The proposed tetrahedral geometry around the metal ions studied were concluded from these measurements. [M(H2L)(H2O)2]Cl2 [M(H2L)(HA)]Molar ratio method were used to determine M to L ratio at maximum wave length (?max=455nm ).The ratio were (1 : 1) mole for both complexes (1) and (4). Bentonite clay was used as adsorbent surface to gather with complexes (1) and (4). The adsorption time investigated was between (15 - 120) min at (25)°C as constant concentration for complexes being (70) mg/L for complex (1) and (50) mg/L for Complex (4) and mesh 75))?m. The results revered that the time needed to reach concentration equilibrium in the were 60 to 75 min for complex (1) and (4). during studying isotherm adsorption for the two complexes on bentonite surface, the results revealed that the trend of the adsorption isotherm was (L1) type according to Giles classification , which means that the adsorbent distributed horizontally on the bentonite surface to from a single adsorption layer type langmire.The adsorption phenomenon was studied at different temperatures (10, 25, 37.5, and 50) oC. The results of the adsorption for both complexes on bentonite surface were showed an increase with increasing temperature (endothermic process). Thermodynamic functions (?Ho, ?Go, and ?So) were calculated and the results were discussed. Generally, the increase in the pH of solution was showed an increase in quantity of both complexes that adsorbed on bentonite clay surface at a certain temperature. The study proved that an increase of adsorbent weight may be enhanced the adsorption process of both complexes. Moreover, the particle size of clay surfaces has an influence on the adsorption process showing an increase in adsorption quantity as the particle size decreased.

تحضير ودراسة طيفية ومعالجة نظرية لمعقدات مزيج ليكاندي مشتقات البنزاميدازول والفينانثرولين

اسم المؤلف: دعاء حبيب محل
اسم المشرف: محاسن فيصل الياس
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء العضوية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الكلمات الدلالية:
  • تحضیر
  • حلقیة غیر متجانسة
  • جالكون، بایرازولین
  • ثنائي هایدروبایرمدین - - 2 اون
  • ثنائي هایدروبایرمدین - 2 - ثایون، بولي ایماید
  • فعالیة بایولوجیة
  • موجات فوق صوتیة Synthesis
  • Heterocyclic
  • Chalcone
  • derivatives
  • Pyrazoline
  • Dihydropyrimidin - 2 - one
الصفحات الاولى:
المستخلص: In this study, two new aromatic chalcones have been synthesized. The first one contain one amino phenyl group while the second contain two amino phenyl groups , from the reaction of 2 - acetothiophene or pamino acetophenone with p - or m - amino benzaldehyde respectively by Claisen - Schmidt condensation in alcoholic base medium (10%NaOH ).Also, some of heterocyclic compounds have been derived from these prepared chalcones as a derivatives for 2 - pyrazoline ring and for dihydro pyrimidine - 2 - one/thione as follows : 1 - Preparation of 3,5 - di (amino phenyl ) - 2 - pyrazoline (3,9) derivatives from condensation of (1,2) chalcone with hydrazine hydrate in absolute ethanol.2 - Preparation of 3,5 - di (amino phenyl) - 1 - phenyl - 2 - pyrazoline (4,10) derivatives by the reaction of chalcone (1,2)with phenyl hydrazine in presence of acetic acid.3 - Preparation of 3,5 - di (amino phenyl) - 1 - substituted - 2 - pyrazoline (5 - 7,11 - 13) from the reaction of chalcones (1,2) with semicarbazide, thiosemicarbazide and isoniazide in strong basic medium (45% NaOH) in absolute ethanol.4 - Preparation of 4,6 - di(amino phenyl ) - 3,4 - dihydro pyrimidine - 2 - one / thion (14,15) : from the reaction of chalcone(2)with urea and thiourea in presence of sodium ethoxide in absolute ethanol in two ways : thermal catalyse and ultrasonic waves with high yield for the last one.The phenyl amines derivatives (3 - 7,9 - 15)included benzidine (8) were converted into N - substituted maleimic acid (16 - 28) , by reacted it with maleic acid anhydride in ether or dry acetone.These derivatives (16 - 28) were undergo dehydration by using anhydrous acetic and sodium acetate to give N - substituted malimide products ( 29 - 41) in good yield.Also, the homopolymers (42 - 54) for these malimides were prepared by free radical polymerization with benzoyl peroxide as initiator and dimethyl formamide (DMF) as a solvent at 130 0C. PDF created with pdfFactory Pro trial version www.pdffactory.com The copolymers (55 - 104)have been prepared by the samemethod and initiator with vinyl acetate for polymers (55 - 67)and with acryl amide for polymers (68 - 72) and with allyl benzene for polymers (73 - 80) and with maleimide (31) for the polymers (81 - 88) , also with maleimide (32) for the polymers (89 - 96) and finally with maleimide (33) for the polymers (97 - 104).The structures of these new compounds were characterized by UV spectra (as a comparable study for unpolymered molecules ),IR (infra - red ) spectra and 1H - NMR (nuclear magnetic resonance ) spectra for some of the prepared compounds and followed by TLC (thin layer chromatography ).The stability of these compounds (9 - 12) were studied spectrally from the changing in its high absorbance values (peaks) ?max for their solutions at concentrations 2×10 - 5M in light and dark with time for comparability. These compounds showed good stability whichcan be suggested to be used as an organic reagents for analytical study especially with drugs and phenolic compounds.More ever, the identification study , here we have studied the heoretical study of formation heat for intermediates and final compounds bodies length of the bond which was agreement with the particularly results.This study ,also includes the biological activity for some of the prepared compounds against four kinds of germs which known by its resistance against antibiotics ,these are Staphylococcus aureus (positive for Gram stain )and Escherichia coli ,Pseudomonas aeruginosa and Salmonalla typhi (negative for Gram stain ) and studiesy the inhibition values for these compounds comparable with three of standard antibiotics as controls (ampicilline : C1, Cloxacilin : C2, erythromycin : C3) , and the results show the ability of these prepared compounds to inhibit all the used germs except Pseudomonas which shows clear resistant for less polarity comparable with control samples.

دراسة تاثير الفسفاتين وبعض المتغيرات الحياتية على مرضى سوفان المفاصل المصابين وغير المصابين بداء السكري من النوع الثاني A Study of Visfatin And Some Biochemical Variables In Osteoarthritis With And Without Diabetes Mellitus Type2

اسم المؤلف: لمياء شاكر عاشور
اسم المشرف: طارق محمد علي رجب الحكيم حامد غفوري حسن
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتضمن هذا الفصل مقدمة عن المركبات الدوائية الاتية وطرائق تقديرها وهي : • الترايفلوبيرازين هيدروكلوريد Trifluoperazine Hydrochloride • طرائق تقدير للترايفلوبيرازين هيدروكلوريد Methods for the determination of Trifluoperazine hydrochloride • الكلوربروم | This thesis consists of four chapters : Chapter one : contains introduction about drug compounds listed below - Trifluoperazine hydrochloride. - Methods for the determination of Trifluoperazine hydrochloride. - Chlorpromazine hydrochloride. - Methods for the determination of Chlorpromazine. - Aim of the research.Chapter two The Chapter includes the development of a new sensitive spectrophotometric method for the determination of a Trifluoperazine hydrochloride in aqueous solution. The method is based on the oxidative coupling reaction of Trifluoperazine hydrochloride with Sulphanilic acid reagent in a acidic medium pH 1.4 in the presence of Potassium Iodate to produce an intense violet coloured, water soluble and stable product , which exhibits maximum absorption at 544 nm. Beer’s law is obeyed over the arange 12 to 66 µg.ml - 1 of Trifluoperazine hydrochloride, with a molar absorptivity of 4804 L.mo1 - 1.cm - 1 , Sandell’s sensitivity index of 0.01 µg.cm - 2 , relative error range not more than 1.26% , and D.L 0.495 µg.ml - 1. The method has been successfully applied for the determination of Trifluoperazine hydrochloride in tablets.Chapter threeThe Chapter includes the development of a sensitive spectrophotometric method for the determination of Trifluoperazine hydrochloride in aqueous solution based on reduction of Fe3+ with Trifluoperazine hydrochloride. The Fe2+ formed is complexed with 2,2’ - bipyridyl at pH 4.2 to produce a red , water soluble and stable complex, which exhibits maximum absorption at 524 nm. Beer’s law obeyed in the concentration range from 2 to 50 µg.ml - 1 of Trifluoperazine hydrochloride. The molar absorptivity is 5284.4 L.mol - 1.cm - 1 and Sandell’s sensitivity index of 0.090 µg. cm - 2 , relative error from 1.64 % , and D.L 0.788 µg.ml - 1. The method has been successfully applied to the determination of Trifluoperazine hydrochloride in tablets.Chapter FourThe Chapter includes the development of a new sensitive spectrophotometric method for the determination of a Chlorpromazine hydrochloride in aqueous solution. The method is based on the oxidative coupling reaction of Chlorpromazine hydrochloride with Sulphanilamide reagent in a acidic medium pH 3.2 in the presence of Ammonium ceric Sulphate dihydrate to produce an intense violet coloured, water soluble and stable dye, which exhibits maximum absorption at 530 nm. Beer’s law is obeyed over the arange 6 to 66 µg.ml - 1 of Chlorpromazine hydrochloride, with a molar absorptivity of 2842.28 L.mo1 - 1.cm - 1 , Sandell’s sensitivity index of 0.1250 µg.cm - 2 , relative error range not more than 2.49 % , and D.L 1.2028 µg.ml - 1. The method has been successfully applied for the determination of Chlorpromazine hydrochloride in tablets.

دراسة حركية لتاثير بعض المركبات المخفضة للدهون الثيازينات والثياديازول على الكرياتين كاينيز و3 - هيدروكسي - 3 - مثيل كلوتاريل كو انزيم - اي ريدكتيز في امصال مرضى ارتفاع الدهون والفئران المختبرية التي تم حث ارتفاع الدهون فيها Kinetic Study of The Effect of Some Novel Lipid Lowering Thiazines And Thiadiazole Compounds On Creatine Kinase And 3 - Hydroxy - 3 - Methyl - Glutaryl - Coa Reductase Activities In Sera of Hyperlipidemia Patient’s And Wister Mice With Induced Hyperlipide

اسم المؤلف: تمارة احمد عبد الكريم العبيدي
اسم المشرف: زينب منيب مالك الربيعي غيد حسان عبد الهادي العبيدي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تمثل الستيرويدات فئة هامة من العقاقير الطبيعية وكذلك الصناعية نظرا لقدرتها على اختراق الخلايا واداء بعض الوظائف البيولوجية الاساسية وبشكل رئيسي هي عوامل مضادة للفيروسات ومضادات للاورام. وتقسم الرسالة الحالية على ثلاثة فصول.يمثل الفصل الاول مقدمة عامة حو | Steroids represent an important class of natural as well as synthetic drugs because of their ability to penetrate cells and perform some of the most fundamental biological functions mainly as antitumor and antiviral agents. The present thesis is devided into three chapters.The first chapter represented a general introduction concerning the structures of some potent steroids, and the pathways of the synthetic adrenal steroids as well as their pharmacological importance in medicine, in general. This part is focused mainly also on the pregnenolone as an important potentially active steroid, including its structural modification at the hydroxyl and keto groups at C - 3 and C - 20 and their uses as antitumor agents. Mitsunobu, Suzuki reactions and aldol condensation as well as chalcon formation and their application at steroids have been described. The second chapter is concerned with the experimental work which included different synthetic methodology.The third chapter is the main part of the thesis, described the synthesis of new 3? - pregnenolone ester derivatives at C - 3 via Misunobu reaction of the carboxylic acid derivatives, such as : rhodamin B, indomethacin, naproxen, protocatecuic acid, vanillic acid and p - coumaric acid, which showed inversion in configuration at the ester group at C - 3. In addition, the synthesis of 17 - (4 - chloro - chalconyl)pregnen - 3? - ol has been described, which then treated with various substituted phenylboronic acids such as : 2,4 - difluoro - , 5 - carboxy - 3 - nitrop - ,4 - fluoro, 4 - thiomethyl - , 4 - hydroxy - , 2,4, - dimethoxy - , 4 - trimethylsilyl, 2 - triflouromethyl - , 3 - cyano, 4 - ethoxyphenyl boronic acids under Suzuki cross - coupling reaction conditions using Pd(PPh3)4 as a catalyst and Na2CO3 as a base to give the (E) - 3 - (substituted - [1,1’ - biphenyl] - 4 - yl) - 1 - (3? - hydroxy - pregnen - 17 - yl) - prop - 2 - en - 1 - one. Two compounds, 17 - acetyl - 5 - pregnen - 3? - yl) - 2 - (2,6 - bis(diethylamino) - 9H - xanthen - 9 - yl)benzoate, and 17 - ((E) - 3 - (4 - chlorophenyl)acryloyl) - 5 - pregnen - 3? - yl) - 2 - (2,6 - bis(diethylamino) - 9H - xanthen - 9 - yl)benzoate have been synthesized via coupling reaction using DCC as a coupling reagent to afford these ester with retention in configuration, aiming to study their fluorescence properties. Moreover, tritylation of the pregnenolone has been described to protect the alcohol at C - 3 during the structural modification of keto group at C - 20 under basic medium. The structures of all the synthesized compounds have been assigned from their 1H, 13C, and 2D NMR (HSQC, HMBC, COSY, NOESY) spectroscopy as well the as theoretical calculations of the HOMO and LUMO energies of the trans and cis isomers of the chalconyl pregnenolone aryl derivatives to compare them with the NMR data, which showed that trans isomer is energetically more favoured.Furthermore, the flourescence proroperties of the two rhodainyl pregnenolone esters have been studies which one show remarkable quantum yield (?F) in comparison to Rhodamin B itself.The anti - HIV activity of some arylated chalconyl pregnenolone derivatives have been studies and one of these analogues having diflouro substituents exhibited remarkable activity against HIV - 1 and 2. Therefore, the molecular modeling study of this analogue is performed and showed two hydrophobic interactions and one hydrogen bonding with the amino acids residues of the reverse transcriptase enzyme of HIV.

تحسين حماية التاكل للمعادن (الخارصين، النحاس، الالمنيوم، الحديد الكربوني والحديد المقاوم للصدا 316) في ماء البحر الصناعي باستخدام الطلاء بالمواد النانوية Corrosion Protection Enhancement Of; Zn, Cu, Al, Carbon Steel And Stainless Steel 316 In Artificial Seawater By Coating With Nanomaterials

اسم المؤلف: رائد عبد شاكر محمود
اسم المشرف: عبد الكريم محمد علي جبر السامرائي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الهدف من هده الدراسة هو تقدير تاثير بعض مشتقات الثياديازول والثيازين الجديدة التحضير على فعالية كل من الانزيمين الكرياتين كاينيز و3 - هيدروكسي - 3 - مثيل كلوتاريل كو - انزيم اي ريدكتيز بالاضافة الى قياس صورة الدهون في مرضى ارتفاع الدهون والفئران المختبرية | The aim of this study is to evaluate the effect of some novel prepared derivatives of thiadiazole and thiazine on the activities of creatine kinase (CK) and 3 - hydroxy - 3 - methylglutaryl CoA reductase(HMGR) in addition to lipid profile in sera of hyperlipidemic patients and in mice induced hyperlipidemia by feeding cholesterol rich diet.The study includes two parts; in vitro study : Sixty individuals with age ranged between (40 - 60) years were enrolled in this study. They were divided into two groups; first group (G1) consists of 30 healthy individuals as a control group with body mass index (BMI) (25.67). The second group (G2)consists of 30 patients with hyperlipidemia and BMI (26.48) which diagnosed by physician. The patients attended the Ibn - Al Naphes hospital during November 2013 to February 2014. Patients with high blood viscosity, diabetes mellitus, renal failure as well as those who are under treatment with statins were excluded. The serum which obtained used in the determination of lipid profile[total cholesterol(Tch),triglyceride(TG), high density lipoprotein(HDL - c), very low density lipoprotein(VLDL - c)], fasting blood glucose(FBG), aspartate transaminase (AST), alanine transaminase(ALT) and C - reactive protein(CRP).Four organic compounds 3 - (4 - (dimethylamino) phenyl) - 2,3 - dihydro - 2 - (3 - nitrophenyl benzo[1,3 - e]thiazin - 4 - one[I], 5 - (4 - imethylamino)benzylideneamino) - 1,3,4 - thiadiazole - 2 - thiol[II], 2 - (4 - dimethylamino)phenyl) - 2,3 - dihydro - 3 - (5 - mercapto - 1,3,4 - thiadiazol - 2 - yl)benzo[1,3 - e]thiazin - 4 - one[III], and N - (4 - (dimethyl amino)benzylidene) - 5 - (isopropylthio) - 1,3,4 - thiadiazole - 2 - amine[IV] were used in this study to test their antihyperlipidaemic ability and their effect on CK and HMGR activities. The results revealed that compounds(III and IV)showed an activation effect in all concentrations on CK and HMGR activities, while compounds(I and II) showed an inhibitory effect in some concentrations for CK and in all concentrations for HMGR. Therefore, compounds (III and IV) were excluded from this study. The results showed that (10 - 4M) for compound I and (10 - 5M) for compounds II give the best inhibition percentage among the other concentrations on CK and HMGR activities which the kinetic study throughout with these concentrations for these compounds. Simvastatin, which considered as standard drug for lipid lowering, was used for comparsion with the potency of compounds I and II on HMGR activity in treatment of hyperlipidaemia. The results showed an inhibitory effect of simvastatin on HMGR activity with percentage inhibition 88%. The effect of compounds (I and II) on ALT and AST were examined in (10 - 4 M) for compound I and (10 - 5M) for compound II in vitro study. The results showed the inhibitory effect of compounds I in concentration (10 - 4 M) and compound II in concentration (10 - 5M) on ALT and AST activities.The Vmax, Km and type of inhibition for compounds I and II on CK and HMGR activities were studied by using Lineweaver - Burk plot. The results showed that also compound I at 10 - 4M was considered to be a noncompetitive inhibitor for CK activity with Vmax values (1000 and 344.82)U/L for uninhibited and inhibited enzyme respectively and Km value (10) mmol/L. The results also showed that compound II at concentration 10 - 5M was considered to be a competitive inhibitor for CK activity with Vmax value (588.23)U/L and Km values (5.51 and 4)mmol/L for the uninhibited and inhibited enzyme respectively.In vitro, the effect of compound (I) with concentration (10 - 4M) and compound (II) with concentration (10 - 5M) were examined in vivo study. The study was carried out with sixty male Wister mice aged seven to eight weeks and theirweight were (180 - 200 g) ,obtained from animal house , in College of Medicine, Baghdad University. The mice were grouped as follow : group one (12 mice) as control group, group(2) : consists of 48 mice in which the mice were daily administered cholesterol (25mg/k/day), in coconut oil 6% and creamy cheese for 28 days. Lipid profile were measured for twelve mice chosen randomly from G2 to diagnosis hyperlipidemia. Then group2 is subdivided into three groups as follows : group (2.A) : (12 mice) as positive control group in which the mice were daily administered simvastatin (40mg /day) as standard drug for hyperlipidemia, group 2B : (12 mice) in which the mice were daily treated with (10 - 4)M of compound (I)via drinking water for 20 days and Group(2.C) : (12 mice) in which the mice were daily treated with (10 - 5)M of compound II for 20 days. The results showed significant elevation in levels of Tch, TG, LDL - c and VLDL - c, while there is significant reduction in HDL - c levels in G2 comparing to control group(G1), after administration of fat rich diet. Simvastatin, compound I with concentration (10 - 4M) and compound II with concentration (10 - 5M) were administrated to G2A, G2B and G2C respectively. Also, the results showed that the activities of CK reduced for group G2B and G2C while it is increased for G2A. The results also showed that the activities of HMGR were reduced in the three treated groups. The results revealed that compounds I and II exhibit more potent antihyperlipidaemic effect than simvastatin. Also, compound I showed more potent antihyperlipidaemic effect than compound II.The results revealed that compounds I and II showed a noncompetitive inhibitor effect on CK with Vmax values(1000and 166.6) U/L for uninhibited and inhibited enzyme respectively and Km value (0.6) mmol/L for compound I, and with Vmax vales (1000 and 250)U/L for uninhibited and inhibited enzyme respectively and Km value (0.84) mmol/L for compound II.In conclusion, the novel synthetic compounds (I and II) seem to be of interest in the development of new antihyperlipidaemic agents that exhibit inhibition effect on CK while statins cause increase in this enzyme. Also these compounds exhibit inhibition effect on HMGR activity more than simvastatin, which is a key enzyme in cholesterol synthesis.

تحضير مركبات عضوية جديدة ذات صفات بلورية سائلة Preparing of New Organic Compounds With Liquid Crystalline Properties

اسم المؤلف: محمود عبد الستار يحيى القزاز
اسم المشرف: ابتسام خليفة جاسم
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الفيزيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يهدف هذا العمل لدراسة امكانية طلاء سطوح بعض المعادن مثل الفولاذ المقاوم للصدا 316، الحديد الكربوني، النحاس، الالمنيوم والزنك، بالمواد النانوية لتحسين مقاومة التاكل وذلك باستخدام اثنين من التقنيات، الاولى بواسطة الطلاء بالالياف النانوية لمادة متعدد الانيلي | This work aims to study the possibility of coating the surfaces of some metals such as stainless steel 316, carbon steel, copper, aluminum, zinc, with nanomaterials to improve the corrosion resistance using two techniques, first by coating with polyaniline nanofiber using electropolymerization of aniline monomer and the second technique by TiO2 nanoparticles via sol - gel process starting from titanium isopropoxide. The work focused on getting thin and homogeneous layer to cover the entire surface of the metal and protect it from corrosion in artificial seawater solution (3.5% NaCl) at 293, 303, 313, and 323 K.Atomic Force Microscope (AFM), Scanning Electron Microscope (SEM), Energy dispersive x - ray (EDX), x - ray photoelectronic spectroscopy (XPS), and x - ray diffraction (XRD) were used to diagnose and describe the structure and morphology of the layers that cover the surface of the metals under investigation, and finally the corrosion parameters ;corrosion potential (Ecorr), corrosion current (Icorr), protection efficiency (PE%), polarization resistance (Rp) and the effect of temperature on the inhibition efficiency in the absence and presence of polyaniline (PANI) or TiO2 coating at temperature ranging from 293 to 323 K were obtained and expressed as; Ea , ?H*, ?S*, and ?G* of all samples were estimated from Tafel plots using potentiostatic technique.The SEM and AFM images of PANI films reflect nanofibers (diameter from 50 to 70 nm); the thicknesses were measured by special AFM scans. They showed values between )700 to 1564 nm(, which may be attributed to the differences in oxidation - reduction potentials of the metals. The XRD patterns of the deposited PANI showed duplicate broad scattering peaks around 2? of 20? and 25?.The AFM images of TiO2 nanoparticles deposited on specimens revealed that the layer was extremely smooth, the particles are small spherical and the average diameter between 26 and 38 nm.The shifts in Ecorr of the uncoated with comparison of the PANI or TiO2 coated samples were small for; S.S316, C.S, Cu, Al, and Zn which means that the anodic and cathodic reactions affected by the same extent.The Rp of the PANI and TiO2 coated substrate was always more than the uncoated ones that attributed to the weak conductivity of PANI and the semiconducting properties of the Titania. All coated metals with PANI or TiO2 exhibited good degree of corrosion protectiveness. For PANI the PE ranged between 56 and 92 % and the best results was achieved for Zn metal this may attributed to the high corrosion values of the bared Zn, while TiO2 coating showed PE at the range of 68 and 93% and the best was for Al metal. The protection efficiencies showed small changes by increasing the temperature but for TiO2 in some cases the PE somehow increased with increasing the temperature. The data showed that the thermodynamic activation functions (Ea and ?H*) of the corrosion of the coated samples are higher than those of the uncoated ones indicating more energy barrier. The entropy of activation ?S* for the uncoated and coated samples are always negative ,this indicates that the activated complex in the rate determining step represents an association rather than a dissociation step, the measured ?G* values takes positive values and showed almost small change with increasing temperature, indicating that the activated complex was not stable and the probability of its formation decreased somehow with rise in temperature and the ?G* values for coated samples reveal that in the activated corrosion complex becomes less stable as compared to the uncoated specimens.Single cyclic potentiostatic tests conducted at 293 K to follow up the pitting probabilities of each metal in seawater before and after coating. The I - V plots indicated that only SS316 and Aluminium specimens suffer from pitting corrosion and the two types of coating reduces the pitting area of the hysterias loop with pushing the pitting potentials for more positive values

دراسة البلمرة الكهروكيمياوية وخواص حماية التاكل لطلاء متعدد البايرول على الفولاذ الكاربوني والفولاذ المقاوم للصدا An Investigation of Electropolymerization And Corrosion Protection Properties of Polypyrrole Coating On Carbon Steel And Stainless Steel

اسم المؤلف: رواء عباس محمد
اسم المشرف: عبد الكريم محمد علي جبر السامرائي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم تحضير سلسلة من مشتقات 1, 2, 4 - ترايزول بواسطة تفاعلات الغلق ,حضر حامض البنزويك هيدرازايد (1) بواسطة تفاعل مثيل بنزويت مع الهيدرازين ثم تفاعل المركب (1) مع CS2 في محلول كحولي قاعدي ليعطي ملح البوتاسيوم (2). حضر المركب (3) بواسطة غلق ملح البوتاسيوم (2)م | A series of 1,2,4 - triazole derivatives were synthesized by cyclization reaction, the benzoic acid hydrazide (1) was synthesized by reaction of methyl benzoate with hydrazine hydrate then compound (1) was reacted with CS2 in solution of alkali ethanol to give potassium dithiocarbazinate salt (2) , the basic nucleus 4 - amino - 5 - phenyl - 1 - 4H - 1,2,4 - triazole - 3 - thiol (3) was prepared by cyclization of potassium salt (2) with hydrazine hydrate using water as solvent under reflux condition. compound (3) was subjected to addition reaction with different aromatic aldehydes to synthesize Schiff bases (4a,b) which were cyclized by treating with thioglycolic acid to prepare compounds (5a,b).compounds (6) and (7) obtained by cyclization reaction of compound (3) with urea and thiourea. Also in this research, 1,3,4 - thiadiazole derivatives were synthesized by cyclization of thiosemicarbazied with substituted carboxylic acid and sulphuric acid, to yield 2 - amino - 5 - R - 1,3,4 - thiadiazole (8). Schiff bases formation (9a,b) were by reflux of aromatic aldehyde with 2 - amino - 5 - R - 1,3,4 - thiadiazole (8) in the presence of absolute ethanol. Compounds (10a,b) were prepared by cyclization reaction of compounds (9a,b) with thioglycolic acid.The Synthesized compound were confirmed by their melting point ,FTIR ,U.V - visible ,1HNMR spectra and evaluated for their antioxidant activity by using stable free radical 1,1 - diphenyl - 2 - picryl - hydrazyl DPPH. of all tested compounds. compound (5b) was the most active in all concentrations compared to standard Ascorbic acid with an IC50 value 5.84 ?g/ml. In this study, the cytotoxic effects for compounds (5a),(5b),(6),(7),(10a),(10b) were studied in one cultured cellular models (MCF7 cell line) breast cancer (at different concentration) compared to doxorubicin as positive control by cell viability assay (MMT assay), compound (5b) showed the highest cytotoxicity effect with an IC50 value =56.98?g/ml.Also, we examine the cytotoxic effects of gold III complex (AuL2) of bi - dentate ligand (5a) in one cultured cellular models (MCF7 cell line) by High Content Screening and analysis (HCS). The inhibitory effect of AuL2 on breast cancer cell growth was due to induction of apoptosis as evidenced by Annexin V staining and cell shrinkage. We found that AuL2 - mediated lead to disruption of mitochondrial membrane potential (MMP), cell membrane permeability, and release of cytochrome c from the mitochondria into the cytosol. suggesting (AuL2 ) as a potential MCF7 inhibitor. Thus, we suggest that (AuL2) may have therapeutic value in breast cancer treatment worthy of further development. Bis(2 - (4 - Dimethylamino - phenyl) - 3 - (3 - mercapto - 5 - phenyl - [1,2,4]triazol - 4 - yl) - thiazolidin - 4 - one)gold(III) chloride. monohydrate

تحضير وتشخيص المركبات الحلقية غير المتجانسة الجديدة والمشتقة من الجالكونات Synthesis And Characterization of New Heterocyclic Compounds Derived From Chalcones

اسم المؤلف: نبراس مظفر جميل
اسم المشرف: جمبد هرمز توما ضحى فاروق حسين
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الفيزيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتقصى البحث امكانية الحصول على طبقة طلاء رقيقة ومتجانسة لبوليمر البايرول على بعض السبائك مثل الفولاذ المقاوم للصدا 316 والفولاذ الكربوني بواسطة البلمرة الكهروكيميائية لمونمر البايرول ودراسة امكانية تحسين مقاومة التاكل في مختلف الاوساط البيئية مثل المياه ا | This research focuses on getting thin and homogeneous polypyrrole (PPy) layer on carbon steel (45) and stainless steel (316) alloys by electropolymeriztion procedure of pyrrole monomer, and study the possibility of improving the corrosion resistance in different medias; saline water (3.5% NaCl), acidic media (1M H2SO4) and basic media (1M NaOH) at different temperature of 293, 303, 313 and 323 K. The deposited PPy layer on samples under investigation were examined by Atomic Force Microscope (AFM), Scanning Electron Microscope (SEM), Visible and Ultraviolet Spectroscopy (UV - Vis), Fourier Transform Infrared Spectroscopy (FT - IR), Thermogravimetric Analysis (TGA) and X - Ray Diffraction (XRD).The corrosion parameters are including corrosion potential (Ecorr), corrosion current (icorr), protection efficiency (%PE), polarization resistance (Rp) and the effect of temperature on the inhibition efficiency in the absence and in the presence of PPy coating at temperature ranging from 293 to 323K were obtained and expressed as; Ea, ?H*, ?S*, and ?G* of all samples were estimated from Tafel plots using potentiostatic technique.The SEM and AFM images of PPy films revealed a nanofiber like structure; diameter from 20 to 120 nm, the thicknesses were measured by gravimetric and special AFM scans, they showed values between 1300 to 1450 nm. UV - Vis studies showed that the PPy films exhibit weaker absorption peak at 477 nm and stronger absorption peak at 906 nm and the XRD patterns of the deposited PPy show two peaks around 2? of (43o) and (50o).The shift in Ecorr of the uncoated and of the PPy coated samples are between ( - 600 to - 200 mV) and ( - 200 to 100mV) for CS and SS316 respectively.The protection effectiveness of all PPy coated specimens showed remarkable degree of enhancement, and the %PE ranged between (30 to 80%) for CS and (78 to 96%) for SS316 in all medias.The values of Ea and ?H* of the coated PPy metals are always higher than the uncoated ones which indicate more energy barrier. The negative entropy ?S* for the uncoated and coated samples indicates that the activated complex in the rate determining step represents an association rather than a dissociation step, the measured ?G* values takes positive values and showed almost small change with increasing temperature, indicating that the activated complex was not stable and the probability of its formation decreased somehow with rise in temperature and the ?G* values for coated samples reveal that in the activated corrosion complex becomes less stable as compared to the uncoated specimens.Single cyclic potentiodynamic tests conducted at 293 K to follow up the pitting probabilities of each alloy in all medias before and after coating, the I - V plots indicated that only SS316 in salty media specimens suffer from pitting corrosion and the PPy coating reduces the pitting area of the hysterias loop with pushing the pitting potentials for more positive values

تقدير التعكرية لفصائل منتخبة باستخدام التشعيع بمصفوفة من ثنائي وصلة باعث وبمتحسسات شمسية بتقنية الحقن الجرياني المستمر : دراسة وتطبيق Turbidimetric Determination of Some Selected Species Via The Use of Linear Array Light Emitting Diode Irradiation With Solar Cell In Continuous Flow Injection Analysis : Study And Application

اسم المؤلف: محمد كاظم حمود
اسم المشرف: عصام محمد علي شاكر الهاشمي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: في هذا البحث تم تقدير المركبات(SMX,TMP,CAF,HYO and PAR) باستخدام المشتقات الطيفية (الاولى , الثانية ,الثالثة والرابعة). على شكل امزجة ثنائية للادوية بواسطة تطبيق التقاطع الصفري كما يلي : 1 - مزيج السلفاميثاكسازول والترايميثوبريم باستخدام المشتقة الاولى و| This research includes determination of drugs SMX, TMP, CAF, HYO and PAR using derivative spectrophotometry (first, second, third and fourth derivative) were developed for binary mixture by applying zero - crossing technique for pure synthetic mixture and their pharmaceutical formulation as follows : 1. SMX with TMP mixture : SMX was determined by applying 1D&4D teach s at 288.0 and 257.8 nm (zero crossing point of TMP) with linear concentration ranges (2 - 30) and (2 - 25) mg/L , r = 0.9996 and r = 0.9992 LOD = 0.750 and LOD = 0.360 mg/L and TMP was determined by applying 4D teach at 251.5 nm (zero crossing point of SMX) with concentration range (2 - 30) mg/L , r = 0.9995 and LOD = 0.382mg/L. The RSD were 0.255, 0.280 and 1.136 for SMX and TMP respectively and applied for (TRIMOL - 400SMX, 80TMP mg) and (METHOPRIM - 400SMX,80TMP mg).2. PAR with CAF mixture : PAR was determined by applying 3D teach at 275.8 nm (zero crossing point of CAF).With linear concentration range (2 - 35) mg/L , r = 0.9987and LOD=0.445mg/L. And CAF was determined by applying 4D teach at 294.7 nm (zero crossing point of PAR). With linear concentration range (2 - 35) mg/L , r = 0.9995 and LOD = 0.162 mg/L. The RSD was 0.222 for PAR and 0.130 for CAF and applied for (PANADOL EXTRA - 500PAR, 65CAF mg).3. PAR with HYO mixture : PAR was determined by applying 1D&2D teach s at 297.4 and 303.5 nm (zero crossing point of HYO) with linear concentration ranges (2 - 30) and (2 - 30) mg/L , r = 0.9998 and r = 0.9987 LOD = 0.081 and LOD = 0.250 mg/L and HYO was determined by applying 1D teach at 215.9 nm (zero crossing point of PAR) with concentration range (2 - 25) mg/L , r = 0.9997 and LOD = 0.091mg/L. The RSD were 0.107, 0.400 and 0.342 for PAR and HYO respectively and applied for (SPAZMOTEK PLUS - 500PAR,10HYO mg). This thesis has mainly been structured in three different chapters, each one containing the following information : Chapter one provides a short historical review with the analytical performance characteristics of UV - visible are described. The applications of UV and DS in pharmaceutical and SMX, TMP, CAF, HYO and PAR analyses and their mixture.the general and specific objectives of thesis are reported. Chapter two corresponds to the experimental part. Reagents, instruments, procedures and detail protocols for the preparation of standard solution and pharmaceutical sample which used in this study are reported.Chapter three contains the experimental results and discussion that lead to the possibility of successful applications which used DS to determine the concentration of each material in drugs.

نمط جديد للتحليل بالحقن الجرياني المستمر باستخدام نظام تشعيع متعدد القنوات مرتبة بهيئة مصفوفة خطية واثنان من الخلايا الشمسية لقياس التعكرية لتقدير الفصائل التحليلية ذات الاهمية : دراسة وتطبيق New Mode of Cfi Analysis Using Multiple Channel Irradiation System In A Linear Array Arrangement With Twin Solar Cells For Turbidimetric Determination of Analytically Interesting Species : Study & Application

اسم المؤلف: عمر اياد ياسين القيسي
اسم المشرف: نغم شاكر تركي العوادي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الفصل الاول : يشمل الفصل الاول مقدمة عن تفاعلات الترسيب، مبادئها، ميكانيكية تكوين الرواسب، انواع الرواسب. وكما يتضمن الفصل الاول مقدمة عن الضوء والنظرية الكهرومغناطسية، وتداخل الضوء مع المادة (الانعكاس، الانكسار، النفاذية، الامتصاص). ناقش الفصل الاول بشكل

دراسة مقارنة ترددات الاهتزاز والصفات الفيزياوية والطاقية لوحدات بناء جزيئة الفلرين و(ZigZag) (SWCNT) باستخدام طريقة ميكانيك الكمي Comparative Study of Vibrational Frequencies, Physical And Energetic Properties For Units Construction of Fullerene Molecule And (ZigZag) (SWCNTs) Using Quantum Mechanical Method

اسم المؤلف: خالدة عبيد سماوي
اسم المشرف: رحاب ماجد كبة
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء التحليلية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Chapter one : Includes an introduction of the light scattering, types of light scattering and its relation ship to the size of precipitate particles which causing the scatter of light. It also involves an introduction to the concept of turbidity and nephelometry for the scattering of incident light, units of both these, as well as an introduction of light emitting diode. This chapter also describes an introduction to flow injection analysis, its principles of the FIA, dispersion, diffusion law , kinds and application and finally followed with the aims of the project.Chapter two : Comprises a complete description for the chemicals used, their preparations in addition to describe the components of flow injection system.Chapter three : consists of two parts : First part : Describes the design of Ayah 6SX1 - T - 2D solar cell CFI Analyser. Also, a full well clear representation of the expected echanism of what might be happened inside the measuring cell for what was measured whether turbidity solutions (measured reflection of incident light by the presence of precipitate particles surface) or a coloured transparent solutions will act normally as absorbance measurement. In second part : A description for the calculation of dilution factor toevaluate the designed manifold system in continuous flow injection analysis via the chasse and following an air bubble through the whole design. Chapter four : Describes the efficiency and its capability of Ayah 6SX1 - T - 2D solar cell CFI Analyser in distinguishing between precipitated and coloured transparent solutions.For precipitate reaction product gives a positive responses by the effect of reflection , this method is used to determine Cd(II) ion. A method is based on the formation of a yellowish white precipitate for the complex Cd3[Fe(CN)6]2.The linearity of Cd (II) ion is ranged from 0.05 to 12 mmol.L - 1, with correlation coefficient r= 0.9951, limit of detection (LOD) 25.29 ng/sample (3SB)(S/N=3) and the percentage relative standard deviation for 4 and 8 mmol.L - 1 less than 1 % (n=5). This method has been applied successfully to determine a Cd (II) ion in a random river samples. Also provided a comparison between the new method with the classical method (HANNA instrument for turbidity measurement) using the standard additions method via the use of ANOVA - treatments. It was noticed that there is a significant difference at ?=0.05 between the two methods at level < 0.05 was obtained.On that basis the new method can be accepted as an alternative analytical method.While a coloured transparent solutions gives a negative responses, this idea is applied for the determination of vanadium (V) ion. This method is based on oxidation of pyrogallol by vanadium (V) ion in acidic meadium to form color species. The linear dynamic range for the instrument response versus vanadium(V) concentration was 1 - 200 mg.L - 1 with correlation coefficient r = 0.9920. The limit of detection (S/N=3) was 70 ng/ sample from the step wise dilution for the minimum concentration in the linear dynamic range of the calibration graph with RSD % for the repeatability lower than 1% for 90 mg.L - 1 (n=5) concentration of vanadium(V). The method was applied successfully for the determination of vanadium (V) in three river samples. A comparison was made between : two methods proposed method with classical method (UV - Vis spectrophotometry at wave length 427 nm) using the standard addition method via the use of paired t - test. It was noticed that there was no significant difference betweentwo methods at 95 % confidence level. Chapter five : Metronidazole (MTZ) and Mebeverine hydrochloride (MVH) are determined in pharmaceutical preparation as alternative analytical procedures. That were developed by continuous flow injection analysis via turbidimetric (T0 - 180 o).For metronidazole (MTZ), the developed method was based on the formation a greenish yellow precipitate as an ion pair complex by reaction between phosphomolybdic acid with metronidazole in aqueous medium. Linear dynamic of metronidazole is ranged from 0.05 - 8 mmol.L - 1,with correlation coefficient r = 0.9821. The limit of detection (S/N= 3 )(3SB)=171.15 ng/sample from the step wise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph with RSD% lower than 0.5% for 4, 4.5 mmol.L - 1 (n=5) concentration of metronidazole. The method was applied successfully for the determination of metronidazole in three pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (HANNA instrument for turbidity measurement) using the standard additionmethod via the use of t - test. It was noticed that there was no significant difference between two methods at 95 % confidence level. For mebeverine hydrochloride (MVH) is determined by formation of a pinkish banana color precipitate as an ion pair complex between Phosphotungstic acid with mebeverine hydrochloride in aqueous medium.The linearity is ranged form 0.05 to 12.5 mmol.L - 1, with correlation oefficient r=0.9966, limit of detection (S/N=3)(3SB)= 521.92 ng/sample and RSD% (n=6) at 2 and 6 mmol.L - 1 MVH concentration less than 1% was obtained. The method was applied successfully for the determination of mebeverine hydrochloride in three pharmaceutical drugs using standard addition method. A comparison was made between the quoted value and the practically found values for three different kinds of drug by t - test. It showedthat there was no significant difference between the quoted value of each individual company with calculated t - value at 95% confidence interval from developed method.Chapter Six : Summarizes a set of conclusions based on the results of this research work which relates to the use of new patterns and methods for the determination of some analytical species by the developed FIA technique, as well as, some future prospects for the homemade FIA system.

التقدير الطيفي لبعض المركبات العضوية المعوضة والمستحضرات الصيدلانية من خلال الاكسدة بايون السيريوم الرباعي باستخدام المحلل الطيفي الدقيق للتحليل بالحقن الجرياني المستمر (Ayah 3S BGR x3 -3D solar cell) Spectrophotometric Determination of Some Substituted Organic Compounds And Pharmaceuticals Formulation Via Their Oxidation With Ce(IV) Ion Using Ayah 3SBGRx3 - 3D Solar Cell CFIA Microphotometer

اسم المؤلف: مالك حسين علي العلوش العامري
اسم المشرف: نغم شاكر تركي العوادي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الفيزيائية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم استخدام حسابات ميكانيك الكم التقريبية شبه التجريبية وفق نموذج الحساب PM3)) وحسابات ميكانيك الكم الاساسية التامة غير التقريبية وفق نظرية دوال الكثافة (DFT) وباسلوب B3LYP وعناصر قاعدة 6 - 311G لحساب جزيئة الفلرين التي امتلكت التماثل Ih مع وحدات بناءها (5 | Semi - empirical quantum mechanical methods (PM3) and Density Functional theory calculations (DFT/ B3LYP/ 6 - 311G) were executed to evaluate the fullerene molecule ,the internal coordinates (bond length and angles) and some physical and energetic properties.Calculations of the geometrical Parameters (bond lengths and bond angles), have shown that the (C=C) bond length is increased with increasing the size of the molecule that increased elasticity especially to fullerene to be like a ball. The (C - C) bond length is decreased with increasing the size of molecule, that means fullerene will be more stable than the other molecules.The energetic values (heat of formation) is found to be increased with the increasing of the size of molecule. The total energy is decreased with increasing size of molecule that means the stability will increase with the increasing of the size of unit construction of fullerene molecule , on the other hand , the energetic difference (E HOMO - LUMO) between the higher level of orbital and the lower level of orbital are decreased with the increasing of the size of molecule ' unit construction reaching to the fullerene molecule. So, this property will increase the significance of fullerene in the industrial and the electrical purposes.The vibrational modes frequencies with ( 3N - 6) whereas N is represented by number of molecule atoms which are evaluated with the absorption intensities of infrared frequencies. symmetrical and coordinated modes were discussed at the equilibrium geometric formation by using the programs MOPAC & Gaussian 05.It is worthy to say that it was not mentioned before that the complementary classification for the vibrated motion modes for such molecules , so our evaluations may considered unique. It is noted , as well , that CH stretching frequency has had fluctuations that means increasing and decreasing in the values of frequencies. In connected with the bending vibrated modes which taking place out of plane of the molecule are decreased with the increasing size of the unit constructions of molecule but it is increased only at the fullerene molecule. Conversely, the bending vibrated modes which are taken place in the plane of molecule are increased with the increasing the size of unit construction of molecule but are decreased at the fullerene molecule.The distribution of the electronic charges density on the atoms of unit construction of fullerene molecule were studied, the results shown the absence of the electronic charge on the atoms of Fullerene molecule and its concentrated at atoms of the outer circumferential edges C - Cc for the aromatic rings in its own unit construction. Semi - empirical methods (PM3) and Density Functional theory calculations (DFT/ B3LYP/ 6 - 311G) were carried out to evaluate the vibration frequencies and infra - red (IR) absorption intensities for equilibrium geometries of construction units of (6,0) zigzag (Mono (D6h), Di (D6d), Tri(D6h), Tetra (D6d)) ring layers SWCNTs and the internal coordinates ( bond length & angles ) at the equilibrium geometric formation symmetrically according to group theory were evaluated. The most important notes were axial bond length C - Ca which is decreased with the increasing of the tube length whether odd or even number of ring layers. The length of these mentioned axial bonds will be longer in the outer layers than the middle layers that means the increasing of stability of the tube with its length increasing , with the ability of replacing the external atoms of the tube because they are connected with longer and weaker bonds. The reverse was found with the circumferential bond lengths C - Cc was as they were increased with the increasing length of the tube whether the tube was odd or even in number of ring layers. Moreover , the lengths of these bonds are decreased in the middle side to the outer one for the odd and even ring layers. This will confirm the stability of the tube with its length increasing. The lengths of outer bonds C - H which are situated on the edges of the tube which were approximately fixed , with exception of the bond length of CH bonds in Mono ring layers which were longer because the large negative charge on carbon to which it is bonded and the large positive charge on it compared with the positive charge on hydrogen atoms of the multi - layers tubes. The heat of formation which was found to be increased with the increasing of the length of the tube , that means increasing of the stability and the aromatic properties , with the increasing of number of tube' layers. This property will increase the importance in the uses of these tubes for the industrial and electrical purposes as semi - conductors.The vibrational modes frequencies with 3N - 6 with the absorption intensities of infrared frequencies were evaluated and classified. These modes were discussed in symmetrical and coordinating manners at the equilibrium geometric formation, by using the programs of MOPAC and Gaussian 05 , with confirming on unavailability of bounded specification for such modes with 3N - 6 in the literature. We have a through our studies that the stretching vibration modes of symmetric and anti - symmetric Carbon - Carbon axial bond (C - Ca) increased with the increasing length of tube , and this will increase the physical properties for electrical conductivity with confirming on the significance of nanotube industrially. For the vibrational bending frequencies which are taking place in plane and out of plane of molecule surface as well as the vibrational stretching for odd number of ring layers (Mono and Tri) fluctuation will be happening in the relations , as all of them will be decreased with the increasing of tube ' length except (C - Caasym, C - Casym , ? CHsciss. , ? ringasym , ? ringsym and ? ringsym ) which be increased with the increasing the length of tube. The vibrational bending frequencies which are taking place in plane and out of plane of molecule surface for the even number of ring layers ( Di and Tetra layers ) will be decreased with the increasing of tube' length except (? CHsciss.) which is increased with the increasing of tube' length. Furthermore , all vibrational modes which are related to the puckering , breathing and bending movements of clockwise and anti - clockwise according to "group theory" were determined in accurate manner to which deformations are related , which are happened in the tubes , as a result of vibration, that may prevent the flow of electrons' movements and their transitions. The numerical values for the vibrational frequencies which are resulted from Semi - empirical methods (PM3) were acceptable in comparing with the strict calculations , hopping to achieve best values and to be close to the values obtained for the strict calculations (DFT) method to be suited with the basis and standards that are depended internationally. Some of these values were corrected by multiplied by constant scaling factors for such molecules. The results of scaling were good and very close to the experimental and calculated values.The distribution of charges' density on units of construction atoms of (6,0) zigzag nanotube SWCNTs also were studied. The results were consistent with the physical and experimental conductivity properties for such calculated tubes , as the intensity of the electronic charge was concentrated on the outer circumferential edges of atoms C - Cc for the aromatic molecules as well as for nanotubes with its disappearing form outer side towards the middle side of multi - ring layers ( Mono , Di , Tri and Tetra ).

تحضير ودراسة مشتقات 2 - امينو - 5 - ثايول - 4,3,1 - ثايادايازول الجديدة Study And Synthesis of New 2 - Amino - 5 - Thiol - 1,3,4 - Thiadiazole Derivatives

اسم المؤلف: هبه مشتاق احمد علي البدري
اسم المشرف: محمد مهدي صالح الدليمي
الموضوع العام: علوم الكيمياء
السنة: 2007
الموضوع الدقيق: الكيمياء التحليلية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The project conducted in this research work was carried out from October 2013 to May 2014 which leads to this thesis. It full's into five chapters. All these chapters cover up the survey, the practical part of preparing,manufacturing and testing many designs that have been adopted during this research work, and the results were subjected into various mathematic and data treatment.Chapter One : discuses the light (first part) absorption process and describes FIA in general (second part), it is classification and fundamentally involved ideas , the uses and applications of FIA. Chapter one also reviews the use of Ceric sulfate (third part) as a reagent definition, properties, uses and the applications. This chapter ends with the aim of the project.Chapter Two : In this chapter a complete description of the chemicals, their preparations and, using them throughout this project. This chapter also describes the use of flow system with all it is components (i.e. peristaltic pump, connection tubes, junction(Y - junction), six ports injection valve, reaction coil, and measuring readout system).Chapter Three : consists of three parts. The first part describes the design of Ayah 3SBGRx3 - 3D solar cell CFIA microphotometer. The idea of the use with the application of new flow injection microphotometer was build up to measure the responses. Flow injection microphotometer was made based on the use of a solar silicon cells as a detectors, and LEDs as an irradiation sources.The second part deals with calculation of dilution factor. This study was carried out to evaluate the change in concentration at each location of the designed manifold system via the chasse of an air bubble.The third part deals with the analysis carried out in this research work.Three different phenolic compounds (Pyrocatechol, Resorcinol, and Pyrogallol) were subjected by Ceric sulfate as oxidizing agent. A comprehensive detailed study was carried out using Ayah 3SBGRx3 - 3D solar cell CFIA microphotometer, the linear range, limit of detection (L.O.D) and percentage relative standard deviation for repeatability were 5 - 40 mMol.L - 1 , 36.63 ng/sample and < 1.5% successively for Pyrocatechol, and 5 - 40 mMol.L - 1, 17.17 ng/sample, and < 0.7% respectively for Resorcinol. While 5 - 40 mMol.L - 1, 41.61 ng/sample, and < 2 % for Pyrogallol. The method was applied successfully for determination of the mentioned phenolic compounds in pure samples. A comparison was made between the newly developed method and the classical method (UV - Vis spectrophotometry) at wave length 492, 481, and 438 nm for Pyrocatechol, Resorcinol and pyrogallol espectively of analysis using the standard additions method via the use of aired t - test. The obtained results was treated mathematically, it was noticed hat there was no significant difference between two different methods for analysis of three different organic compounds; in addition to no significant difference in the contribution of the Pyrocatechol, Resorcinol and pyrogallol to the oxidation reaction path, at 95% confidence level.Chapter Four : deals with the analysis of two different drugs (first part : Amiloride, and second part Ciprofloxacin) carried out in this research work using Ceric sulfate as a oxidizing agent. A comprehensive detailed study was carried out by Ayah 3SBGRx3 - 3D solar cell CFIA microphotometer, the linear range, limit of detection (L.O.D) and percentage relative standard deviation for repeatability were 0.0005 - 10 mMol.L - 1, 9.471 ng/sample, and < 1.3% successively for Amiloride. 0.05 - 12 mMol.L - 1, 0.300 ?g/sample, and < 0.6%, for Ciprofloxacin. The method was applied successfully for determination of mentioned drugs in pharmaceuticals formulation. A comparison (forAmiloride ) was made between the newly developed method and the classical method (UV - Vis spectrophotometry at wave length 540nm) of analysis using the standard additions method via the use of paired t - test. It showed that there was no significant difference between the quoted value of each individual company with calculated t - value at 95% confidence interval from developed method, in addition to comparison between two methods and calculate tvalue, it was noticed that there was no significant differences between two methods at 99% but a significant difference at 95% confidence level. A comparison (for Ciprofloxacin) was made between the newly developed method and the classical method (UV - Vis spectrophotometry) at wave length 551nm of analysis using the standard additions method via the use of paired t - test. It showed that there was no significant difference between the quoted values of each individual company with calculated t - value at 95% confidence interval from developed method, in addition to comparison between two methods and calculate t - value, it was noticed that there was no significant difference between two methods at 95%.Chapter Five : This chapter concludes few interesting points based on the obtained results throughout this research work, such as Conclusions and future prospect, Published work, and references.

تحضير، وتشخيص ليكاندات ازو جديدة ومعقداتها ودراسة تطبيقاتها Synthesis, Characterization of New Azo Ligands And Their Complexes With Application Study

اسم المؤلف: زينب عبد الهادي حسن علي
اسم المشرف: عباس علي صالح الحمداني
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء الحياتية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في البيت الحيواني التابع لكلية الطب البيطري/جامعة القادسية على ستين جرذا من الذكور ذات وزن يتراوح بين (156 - 160) غم قسمت على اربع مجموعات : المجموعة الاولى (السيطرة), المجموعة الثانية (المصابة بداء السكري) بعد 24 يوما من بداية التجربة, | This study was conducted in the house of the Faculty of Veterinary Medicine Animal/University of Al - Qadisiya on 60 a rat of males of the weight of the ranges between (156 - 160) gm. divided into four groups : the first group (control), second group (diabetes) after 24 days from the beginning of the experiment, the third groups (Pre - treatment) by local EEP for 24 days and then was to stimulate diabetes where once, the fourth group (Post - treatment) by local EEP is that to stimulate diabetes after 24 days from the beginning of the experiment. doses extracted from local EEP be 300 mg/kg of body weight.At the end of the experiment was sacrificed animal blood serum samples collected from rats under anesthesia to measure changes biochemical , as well as the extraction of kidney samples for examination PCR - SSCP for candidate genes in diabetic nephropathy and examination microscopically for kidney samples in the four groups. The results obtained PCR - SSCP technology a surge in certain sites in genes (GLUT1 and nephrin promoter) in diabetes infected groups (G2, G3, G4) compared to control group (G1) where he found and local EEP not corrected of mutations found in both two gene signature, which indicates an extracted from local EEP is not an effective impact in reducing genotoxicity for STZ but an effective impact on the reactive nitrogen species where found a decreases in peroxynitrite concentration in treatment groups through disclosure of 3 - nitrotyrosine by ELISA technology. As well as the decline in the level of nitric oxide radical in the serum (P<0.05).As reveal the incidence of kidney failure by measuring the level of each of creatinin and uric acid in serum. It was noted at the end of the search low moral in body weight, and an increase in the level of moral glucose in blood and increasing the level of uric acid and creatinin, in the serum (P<0.05), for the group infected with diabetes. While groups that treated by local EEP processing had a clear impact in reducing the level of glucose, Nitrotyrosin, nitric oxide, Uric acid and Creatinine. conclusion can be local EEP has characteristics of local anti - oxidization also through its impact in reducing sugar level in blood and its impact on the moderate on kidney functions through the reduction of creatinine and uric acid concentration in blood through improving of histological changes in kidney rats that suffer from diabetes resulting from STZ as well as to the impact of immunological.

دراسة فيزيائية لتشخيص سطح البوكسايت والبوكسايت المعدل لامتزاز مادة التتراسيكلين عليهما عند درجات حرارة مختلفة Physical Study of Bauxite And Modified Bauxite Surfaces Identification For Tetracycline Adsorption On Them At Different Temperatures

اسم المؤلف: اسراء محمد راضي
اسم المشرف: تقي الدين عبد الهادي حمدان
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمن البحث تحضير وتشخيص استرات وبوليمرات مختلفة جديدة عن طريق تحضير مركبات الكيلية اوروماتية حاملة لمجاميع الهيدروكسيل عن طريق مفاعلة الكليسيرول مع (الريسورسينول , الكاتيكول , الهايدروكوينون ) بشكل منفصل.تم اجراء دراسة نظرية لنواتج الالكلة وبعض نواتج ال | This research includes the synthesis and Identification of new esters and polymers via new alkyl Aromatic containing hydroxyl groups. These compounds was prepared by reacting of glycerol with (resorcinol, catechol, hydroquinone) separately.A theoretical study For the products of alkylation and Polymers was conducted and compared with results.This Work includes the following : - first step : - Alkylation of cyclic compounds (resorcinol, catechol, hydroquinone) with glycerol to produce.2 - (2,4 - dihydroxyphenyl)propane - 1,2,3triol (H1) 2 - (2,5 - dihydroxyphenyl)propane - 1,2,3triol (H2) 2 - (3,4 - dihydroxyphenyl)propane - 1,2,3triol (H3)The resulting compounds were Identified by different techniques (FT - infrared spectra IR, NMR spectrum magnetic (1H - NMR, 13C - NMR) and Elemental analysis (CHN).Some physical properties of these compounds were studied.Step Two : - This step in clndes the preparation of ester compounds (Es1 - Es6) via reacting (H1, H2, H3) with Dicarboxylic Acid (malonic, Succinic) The resulting compounds were identified by different techniques physical properties of these compounds were studied Step Three : - Compounds (H1,H2,H3)were polymerized to produce new polymers (p1 - p9) via reacting with anhydride (maleic , phallic and succinic) by melting reaction (200 - 250) C.These Polymers were studied and Identified by different chniqnes including (FT - infrared spectrum, DSC, DLS, and studying solubility in different organic solvents, and measuring the percentage of swelling.Step Four : - The theoretical simulation of alkylation reaction of compounds (resorcinol, hydroquinone and catechol) with glycerol in existence of silicate aluminum were condncted. The transitional situation of these reactants and estimated energy values that leads to the suggestion interactive pathways, where studied. The prepared compounds (H1, H2, H3) were found haning a low value of total energy ( - 390.240 , - 390.232, - 390.251kCal \ mol), respectively, The study also these compounds possass less ? gab values (1.515,1.23, 0.68ev) respectively which was calculated density function calculations of DFT (minimal - STO - 3G).The comparison between the theoretical and obtained values of these compounds were conducted.The theoretical simulation of polymerization reaction (H1, H2, H3) to produce the polymers (p1,p4,p7)were done.The third is the most possible be cause it has the lowest values of total energy( - 126.625, - 126.491 , - 125.814 kCal\mol), respectively, and also has the lowest ?gab values (8.31,7.03.7.088ev).Polymers (p1,p4,p7) are theoretical produced by ring closure reaction and reaction and the possible energy values of these reactants are ( - 222.444, - 222.443, - 190288.29 kCal \ mol) calculated by semi - empirical calculations PM3.in conclusion, these polymers are formed through condensation reaction but not cyclic closure reaction.

تقدير بعض الملوثات العضوية وازالتها من محاليلها المائية باستخدام اطيان عراقية واطيان عراقية محورة وتطبيق برنامج شبه تجريبي لدراسة الامتزاز نظريا Determination And Removal of Some Organic Pollutants From Aqueous Solution By Using Iraqi Clays, Modified Clays And Applied Semi - Empirical Program To Theoretical Adsorption Study

اسم المؤلف: اقبال سلمان محمد
اسم المشرف: سعدية احمد ظاهر خلود عبد صالح
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم في هذه الدراسة تحضير معقدات مخلبية للموليبدنيوم (V) مع ليكاندات ثنائي الالكيل ثنائي ثايوكاربامات وتشخيصها طيفيا" باستخدام مطيافية الاشعة المرئية والفوق البنفسجية ومطيافية الاشعة تحت الحمراء, وهذه المعقدات هي : 1 - فنيل هايدرازايدو - ثلاثي (ثنائي مثيل ث | This study included Preparation of chelate molybdenum(V) complexes with diethyldithiocarbat ligands and characterized them by (UV - Visible) and infrared (FTIR) spectroscopy.These complexes are : 1 - Phenyl hydrazido - tris - dimethyldithiocarbato molybdenum(V) [Mo(N2ph)(S2CNMe2)3].2 - Phenyl hydrazido - tris - diethyldithiocarbato molybdenum(V) [Mo(N2ph)(S2CNEt2)3].3 - (2,4 - dinitro - Phenyl hydrazido) - tris (diMethyldithiocarbamato molybdenum(V). [ Mo( N2ph - (NO2 )2) (S2CNMe2)3 ]. 4 - (2,4 - dinitro - Phenyl hydrazido) - tris (diethyldithiocarbamato molybdenum(V). [ Mo( N2ph - (NO2 )2) (S2CNEt2)3 ].The study of the kinetics of chemical decomposition for these chelates complexes by using irradiation of these complexes solution, by using a mono wave light (? = 365nm) and temperature (25?C) for (60 min) in ethanol solvent. The result for all complexes were that the order of photochemical decomposition was first order reaction. As well as the rate constants of photo decomposition reactions (kd) were calculated by observed a spectral changes during irradiation process. To know the best conditions for decomposition of these complexes, it is necessary to study the effect of several factors on photo chemical behavior for complexes which included : Effect of concentration of complexes, where five concentrations of complexes have been studied and found that the highest decomposition rate have observed at lowest concentration (2.5x10 - 5M). In addition, study the impact of change in intensity of light on decomposition rate. The result was when the light intensity increase, rate of complexes decomposition also increased. Also study a decomposition of complexs at temperature range (25 - 40?C). The result was when increasing temperature, the rate of complexs decomposition was increas, also the values of activated energy of all complexes where calculated and the results where show in the table : Activated Energy (kj / mole) Complex24.808 B122.971 C120.209 B217.778 C2 Also study effect of pH value at ranging (3 - 11) and found that the rate of the complexes decomposition increased when pH of solutions decreases. The effect of four alcoholic solvent were studied such as (methanol, ethanol, 1 - butanol and isopropanol), and found that the highest decomposition rate of these complexes achieved in Methanol, while the lowest decomposition rate of these complexes achieved in Isopropanol. In addition study the effect of each of nitrogen gas, oxygen gas and the atmospheric air on the rate of complexes decomposition and compile the results with recorded results in normal conditions, the highest decomposition rate observed in presence of oxygen gas and the lowest decomposition in presence of nitrogen gas. The mechanism of photo chemical decomposition of these complexes has been interpreted by spectrophotometric technique, were the conclusion that have been obtained that there is (redox - reaction) happening in these complexes and the output of processes of homo cleavage of bond (M - L) that leading to reduction of metal ions and oxygenation of ligand and formation free radicals.

هرمون الانتي مولرين كدالة لمتلازمة تكيس المبايض في امصال النساء العراقيات Serum Anti - Mullerian Hormone As A Marker of Polycystic Ovary Syndrome In Iraqi Women

اسم المؤلف: شهد فوزي عبيد عبد الواحد الخفاجي
اسم المشرف: بشرى فارس حسن غريد خليل محمد علي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتضمن البحث تنقية المحاليل المائية المخففة من الملوثات العضوية صبغة الرودامين وصبغة الكونغو الاحمر بطريقتين الطريقة الاولى كانت امتزاز هذه الصبغات على سطح البتونايت ضمن Al2O3، ZnO،TiO 005 ( جزء بالمليون استعلمت المركبات الثانوية 2 - مدى من التراكيز ) 05 ) | The present work deals with the purification of diluted aqueous solutions from organic pollutants Rhodamine B (RhB) and Congo red (CR) dyes with two methods. The first one was adsorption these dyes on bentonite in range (50 - 250)mgl - 1, Nanocompounds,TiO2, ZnO, Al2O3 and quaternary ammonium salts Hexadecyl tri methyl ammonium bromide (HDMAB), long chain hydrocarbon compounds Sodium dodecyl sulfate(SDS) in different amounts (0.01 - 0.1)g/10g of bentonite were used to modify the adsorption capacity to remove the RhB and CR dyes from aqueous solutions. Both bentonite and modified bentonite are firstly characterized using SEM, AFM and FTIR techniques.The effect of dyes concentration, contact time, effect of initial dye concentration and contact time, adsorbent dosage, and amount of modifiers on the percentage removal have been studied. The modified bentonite with SDS. TiO2 , ZnO increased the percentage removal(R%) of RhB dye from 80% by natural bentonite to 99.3%,98.9% and98.7% respectively, the other dye CR modified bentonite with HDMAB,TiO2 ,ZnO, increased the R% from 81.3% to 99.6%, 99.0% and 98.8%,while adding Al2O3 did not success to increase the R% of RhB and CR on bentonite surface The study exhibits that Langmuir and Freundlich models gave geed fitted to the experimental adsorption at 25°C.The second method deals with advanced oxidation process by using photo - Fenton catalyst(Fe2+,H2O2 and UV light) to degradates of two dyes RhB and CR, the effect of Fe2+ concentration,H2O2 concentration , initial dye concentration and effect of pH at different interval time have been studied.results indicated that simultaneous utilization of UV irradiation with Fentons reagent increase the degradation degree of RhB and CR dyes quickly lose their color, indicating that the dissolves organics have been oxidized, the maximum color removal were obtained at pH 2.0,250 mgl - 1of Fe2+,300 mgl - 1of H2O2for RhB at 240 minute while for CR maximum removal were at pH 3.0,200 mgl - 1of Fe+2,350 mgl - 1of H2O2 at 270 minute.The photo - Fenton degradation process was monitored by HPLC chromatogram of RhB and CR dye solutions. The intensity of the peaks are gradually decreased with irradiation time.The irradiation time after three hours show no HPLC peaks detected, the results are in a good agreement with the results obtained from the UV visible spectrum.In this study semi - empirical methods are used to calculate the molecular orbital energies. This calculation let to suggest the best surface active material which can be used to modify the adsorption efficiency for RhB and CR on Iraqi bentonite surface. The theoretical study results are compared to experimental results achieved in experimental studies on some organic pollutants and modified bentonite by SDS and HDMAB. Using SDS led to the best adsorption efficiency for RhB while HDMAB lead to best efficiency for CR dye. The enthalpy of formation, dipole moment and energy of molecular orbital HOMO and LUMO energies levels were calculated for the two dyes and the two modifiers.

التقدير بتقنية الحقن الجرياني المستمر التعكرية لايون الاوكسونيوم من خلال مبادل ايواني واستعمال مصفوفة خطية للتشعيع بنظام فوتومتري 5SX1 - T - 1D وباستعمال خلية شمسية كمتحسس Turbidimetric - Cfia Determination of Oxonium Ion Through Ion Exchange Resin With The Use of Linear Array Ayah 5Sx1 - T - 1D Solar Microphotometer

اسم المؤلف: احمد ازهر منصور الصراف
اسم المشرف: عصام محمد علي شاكر الهاشمي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الحياتية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: لوحظ في الفترة الاخيرة ظهور مرض متلازمة تكيس المبايض في الاناث خلال سن الانجاب، ان نشوء المرض ربما يعود للاختلال الهرمونات ( المبايض، الغدة النخامية ) ومقاومة الانسولين. ومؤخرا تم استخدام هرمون الانتي - مولرين كمؤشر في التشخيص الدقيق لمرض متلازمة تكيس | In the recent period there had been increased emergence of polycystic ovary syndrome in females during the reproductive age, which may be due to hormonal imbalances (ovaries, pituitary gland) and insulin resistance. Recently Anti - Mullerian hormone had been used as an indicator for determining the degree and accurate diagnosis of polycystic ovary syndrome in serum of patients as well as the measurement fasting insulin and other hormones routine.Aim of study : - ? Proof that anti - mullerian hormone is a marker for diagnosis of polycystic ovary syndrome patients. ? Compares hormones level as : anti mullerian hormone, fasting insulin, luteinizing hormone, follicle stimulation hormone, Prolactin, testosterone between polycystic ovary syndrome and normo - ovulatory women. ? Correlate the level of anti mullerian hormone with other the biochemical features as hormones follicle stimulation hormone, luteinizing hormone, testosterone, prolactin and insulin resistance.? To find a cut - off value for anti - mullerian hormone in Iraqi women with polycystic ovary syndrome. Subjects and Methods : - Forty patients with polycystic ovary syndrome , aged ( 20 - 35) years attending AL - Seweraa hospital / Waset , Kamal AL - Samarai hospital / Baghdad for infertility and gynecology and Al Elwiya Maternity Teaching Hospital were included in the present study , also forty normal fertile females ,aged (21 - 37) years who serve as control group. Blood samples were taken from all subjects from day 2 - 5 day of menstrual cycle. The level of all parameters were quantitatively determined in patients and control subjects by Vidas. Except Anti - mullerian hormone and insulin hormone quantitatively determined in patients and control subjects by Enzyme - Linked Immuno Assay [ELISA]. Test using commercially available Kits as well as the important measurements that had been done include body mass Index.Results : - ? There was a significant [P = 0.0001] increase in Anti mullerian hormone in polycystic ovary syndrome patients compared to controls.? There was a significant [P = 0.0001] increase in luteinizing hormone in polycystic ovary syndrome patients compared to controls , while the level of follicle stimulation hormone and fasting serum glucose was found to be insignificantly when compared with control group.? There was a significant [P = 0.001] increases in testosterone and prolactin hormones in polycystic ovary syndrome patients compared to controls.? Anti - mullerian hormone is more sensitive and specific than the other tests in predicting the occurrence of the disease with a cut off value (>7.9). ? The mean serum level of fasting insulin, homeostatic model assessment was significantly [P=0.0001] elevated in the polycystic ovary syndrome patients when compared to that found in the control group.

تحضير وتشخيص بعض المشتقات الجديدة للبنزايميدازول المعوضة Synthesis And Characterization of Some New Substituted Benzimidazole Derivatives

اسم المؤلف: سحر ثامر عداي
اسم المشرف: عبد الامير مطلك فنجان
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء التحليلية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The project conducted in this research work was carried out from December 2013 to June 2014 which leads to this thesis, which are in five chapters. All these chapters cover up the survey, the practical part of preparing, manufacturing and using Atomic force Microscopy that have been adopted during this research work, and the results were subjected into various internationally used mathematical and data treatments.Chapter OneDiscusses the acids, ion exchange, precipitation reaction, crystal growth theories, light scattering, turbidity and describe FIA in general, it is classification and fundamentally involved ideas without forgetting the use and applications of FIA. Chapter one also discusses derivative spectroscopy, definition, classification, the applications. This chapter ends with the aim of the project.Chapter Two : In this chapter a complete description of the chemicals and ion exchange column, their preparations and their use throughout this project. This chapter describes the use of flow system with all it is components (i.e. peristaltic pump, connection tubes, junction(Y - junction), six ports injection valve, ion exchange column and measuring readout system). Chapter Three : Consists of four parts The first part describes the design of Ayah 5SX1 - T - 1D Solar cell CFIA and deals with calculation of dispersion to evaluate the change in concentration ateach location of the designed manifold system via the chasse of an air bubble. The second part deals with the analysis carried out in this research work. Determination of oxonium ion (HClO4, HNO3, H2SO4 and HCl) via turbidity of solution of zinc (II) ion in cation exchange column - K3[Fe(CN)6] system. A comprehensive detailed study was carried out using Ayah 5SX1 - T - 1D Solar cell CFIA microphotometer. The linear range (10 - 100 mMol.L - 1), limit of detection (43.20, 57.14, 30.28, 30.97 ng/sample) for above mentioned acids and percentage of relative standard deviation < 2% for five successive measurement of 20 and 80 mMol.L - 1 for used acids. The method was applied successfully for determination of the mentioned oxonium ion in pure and commercial samples. A comparison was made between the newly developed method and the classical method pH meter using of paired t - test. It was noticed that there was no significant difference between two methods.The third part deals with the analysis carried out in this research work. Determination of oxonium ion (HClO4, HNO3, H2SO4, HCl) via turbidity of solution of copper (II) ion in cation exchange column - K3[Fe(CN)6] system. A comprehensive detailed study was carried out using Ayah 5SX1 - T - 1D Solar cell CFIA microphotometer. The linear range (10 - 100) mMol.L - 1, limit of detection (42.94, 39.14, 20.61, 26.11 ng/sample) for above mentioned acids and percentage of relative standard deviation < 2.5% for five successive measurement of 20 and 100 mMol.L - 1 for used. The method was applied successfully for determination of the mentioned oxonium ion in pure and commercial samples. A comparison was made between the newly developed method and the classical method pH meter using paired t - test. It was noticed that there was no significant difference between two methods.The fourth part deals with the approache that was used through linear array Ayah 5SX1 - T - 1D continuous flow injection analyzer to distinguish between the responses that were obtained i.e. discrimination between the kind signals that were completely different.Chapter FourDeals with studying topography and morphography of the Zn3[Fe(CN)6]2 and Cu3[Fe(CN)6]2 complexes using atomic force microscopy. Chapter Five : This chapter concludes few interesting points based on the obtained results throughout this research work, such as conclusions and future work, Published work, and references. -

تقدير السبروفلوكساسين, النورفلوكساسين والحديد الثلاثي باستعمال الطرق الطيفية مع الاستخلاص بنقطة الغيمة Determination of Ciprofloxacin, Norfloxacin And Iron (III) Using Spectroscopic Methods With Cloud Point Extraction

اسم المؤلف: نورا سعد مبدر
اسم المشرف: زهير عبد الامير خماس
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء العضوية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتضمن البحث تحضير ستة وثلاثون مشتق جديد من مشتقات]2 - (5 - كلورو - 1H - بنزو]د[ايميدازول - 2 - يل انيلين[، وقد تمت دراستها وتشخيصها بواسطة اجراء تحاليل اطياف الاشعه تحت الحمراء FT - IR)) واطياف الرنين النووي المغناطيسي للبروتونH - NMR)1) لها.تم تقسيم تح | This research includes synthesis of thirty six new derivatives of [2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl)aniline] and these derivatives were characterized by their FT - IR and 1H - NMR spectra.The synthesized compounds were divided into three parts : - The first part involved : - 1 - Synthesis of 2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl) aniline [1] by the reaction of 4 - chloro - 1,2 - diaminobenzene with anthranilic acid and hydrochloric acid as a reagent.2 - Acetylation of compound [1] by using acetic anhydride in order to obtaine the Acetamide compound [2]. 3 - Synthesis of N - [2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl) phenyl) - hydroxyl N' - methyl] acetimidamide [3] by the reaction of compound [2]with hydroxyl amine hydrochloride and sodium carbonate.4 - Synthesis of Benzimidazolyl Chalcone derivatives [4 - 11] by the reaction of the compound [2] with various aromatic aldehydes such as benzaldehyde ,4 - nitro benzaldehyde,4 - chloro benzaldehyde and 4 - methyl benzaldehyde.The second part included : Mannich reaction was carried out based on the compound [1] using formaldehyde and different secondary amines such as piperdine , indole, diphenyl amine , morpholine , indole - 3 - acetic acid , and imidazole to offered [12 - 24]. The third part was synthesized as following : - 1 - Synthesis of Schiff bases [25 - 32] from the reaction of compound [1] with various aldehydes such as benzaldehyde, 4 - bromobenzaldehyde, 4 - hydroxy benzaldehyde, and glutar aldehyde.2 - Reaction of Schiff bases [26 - 29] with mercaptoacetic acid to give new compounds containing thiazolidin ring [33 - 36].

دراسة التاكل والحماية من التاكل لسبائك من حديد الفولاذ في ماء البحر باستخدام دقائق اوكسيد الزركونيوم وكربيد السليكون واوكسيد الالمنيوم النانوية Corrosion And Corrosion Protection Studies of Carbon Steel Alloy In Seawater Using; Zirconia, Silicon Carbide And Alumina Nanoparticles

اسم المؤلف: حيدر عبد الكريم يوسف
اسم المشرف: خلود عبد صالح السعدي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء الفيزيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعتبر اوكسيد النحاسوز واحدا من المواد ذات الاستخدامات العلمية المتعددة الاغراض وذات الاهمية الصناعية الكبيرة ,ومنها استخدامه كمتحسس للغازات وفي العوامل المساعدة وكذلك في انتاج الواح الطاقة الشمسية ويدخل وبصورة كبيرة كمحطم للصبغات العضوية في مجال الكيمياء | Cuprous oxide (Cu2O) has been extensively studied because of its potential use in several electronic applications, which include solar cells and gas sensors. This Thesis is devoted to the synthesis, characterization of nano - structured cuprous oxide by using a simple electrochemical cell with a potassium chloride solution as an electrolyte, the electro cell contains a two high purity copper electrodes as a sheet form in different areas at varying sizes the area of the cathode was twice of the area of anode , the two electrodes zone were separated using a fine diameter silica diaphragm,Within this broad scope , the Thesis focuses on : • Production of different shape nano - crystals (Cu2O) and investigation of the correlation between experimental parameters and resulting microstructure.• Production of highly purity nano - crystalline Cu2O powder, with the estimation it’s by the available investigation instruments.• Study the different parameters, current density, bath temperature, KCl concentration , types of additives , time of precipitation, and there’s effects on the shape ,quantity ,surface roughness, powder color, on the Cu2O powder. Models have been proposed for the nano - crystal formation validated by several techniques uses such as X - ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), and Fourier Transform Infra - Red spectroscopy (FTIR). The produced nanocrystals show good crystallinity with Cu2O purity and Copper oxide semiconductor nanoparticles were prepared by electrochemistry using galvanostatic and potentiostatic approaches. The optimum deposition conditions ( electrolyte composition , medium pH , type of additives, electrodes area ,current density , temperature of the electrolyte and applied potential of the cell) , to prepare the Cu2O nano particles have been identified; in particular the conditions that allow getting this product by using KCl ,(PVA ,SDS ,and glycerin as emulsifying agent) have been well identified for the first time. The configuration of deposited Cu2O , purity and amounts. These values depend on the electro deposition conditions such as the pH of the solution, the deposition potential and temperature. Where the study results showed that there is direct effect of these factors, it has been observed that the sizes of the precipitate crystalline decreased with increasing current density and especially at high bath temperatures its ranged about 10 - 20 nm , in addition to the effect of the percentage of additives emulsifier agents the best results at poly vinyl alcohol.. The X - ray diffraction pattern and 2? values reflect that the high purity of the deposit where obtained, without any of the other undesirable products like CuO, CuCl2 and CuCl. Also the FTIR analysis was considered. the effect of additives of the type of emulsifier on the crystalline form of the deposit, the PVA produced of the Star (Branches) crystals shape, the addition of the SDS compound leads to the form of the cubic , and the glycerin product the binary pyramid crystals shape.. Finally the conditions effect on the surface roughness of the precipitated powder also discussed. Depending of the electro - deposition conditions, different surface morphologies with various preferential crystal orientations were obtained for the temperatures of the electro - deposition of 80 °C , pH = 8 - 9 , The influence of several deposition parameters of the Cu2O, such as applied potential, pH, the temperature of the bath , the chemical composition, grain size and orientation parameters of the sample was systematically studied using X - Ray Diffraction , Atomic Force Microscopy, Transmission Electron Microscopy ,Fourier Transmission Infra - Red, and scanning electron microscopy.

دراسات ثرموداينميكية وحركية لفعالية مركبات متعدد الفينولات الطبيعية للوقاية من التسمم بالرصاص Thermodynamic And Kinetic Studies On The Effectiveness of Nutritional Origin Poly Phenols In Lead Poisoning Protection

اسم المؤلف: نجدت رضا حميد علي الخفاجي
اسم المشرف: الهام مجيد الرفيعي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتضمن البحث تحضير مركبات حلقية غير متجانسة جديدة ( خماسية , سداسية وسباعية )ودراسة الفعالية الحيوية لها.المرحلة الاولى : تتضمن مفاعلة بنزين ثنائي الامين مع ثنائي كبريتيد الكاربون في الايثانول المطلق للحصول على المركب [1] ليتفاعل مع ( حامض الماليك , حام | This research involves synthesis of new heterocyclic compounds(five , six , and seven - membered rings) and study their biological activity First Part : The o - phenylenediamine was condensed with carbon disulfide in abs. ethanol to yield compound [1] which reacted with(maleic acid and oxalic acid ) and presence of phosphorus oxychlorid to yield compounds [2,3].Second Part : The phthalic anhydride was condensed with glycine in aceton to give compound [4] which reacted with o - phenylenediamine in presence of phosphorus oxychlorid to give compounds [5] and with o - phenylenediamine in abs. ethanol to give compound [6] which reacted with maleic acid in presence of phosphorus oxychlorid to give compound [7]. Third Part : Thiosemicarbizide was condensed with carbon disulfide in abs. ethanol and presence of sodium carbonate to result compound [8] which reacted with different sugars (ribose , xylose and arabinose) in abs. ethanol and presence of glacial acetic acid to give new Schiff bases derivatives [9 - 11] respectively. The resulting imine derivatives were reacted with ( salicylic acid , 2 - mercaptobenzoic acid and glycine) to give compounds [12 - 14] respectively.Fourth Part : Study of biological activity of some synthesized compounds

تاثير الصفائح النانوية على الخواص الفيزيائية والكهربائية لبعض المركبات البلورية السائلة Effect of Nanosheets On The Physical And Electrical Properties of Some Liquid Crystalline Compounds

اسم المؤلف: الاء فاضل سليمان داود
اسم المشرف: عصام عبد الكريم عبد اللطيف عمار هاني الدجيلي
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هذه الاطروحة تمثل سلسلة من دراسات الكم من خلال التركيب الالكتروني للانظمة العضوية الفلزية. تتضمن نتائج الفصول خلاصة عمل تعود لكيمياء الروثينيوم (الفصل الثاني) واستخدام النظريات لتساعدنا على التشخيص التركيبي من خلال اجهزة الطيف الحاسوبية (الفصل الثالث والر | This thesis presents a series of quantum chemical studies into the electronic structure of organometallic systems. All of the results chapters are self - contained, summarising work related to Ruthenium chemistry (Chapter 2) and the use of theory to aid structural identification through computation of spectroscopic parameters (Chapters 3 and 4). Chapter 5 relates to distinct work done concerning to the Topological Analysis of the Electron Density in the dihydried Triruthenium Cluster, but the focus on the question of dihydried triruthenium motif represents a close link to the material covered in Chapters 3 and 4. In Chapter 2, Density Functional Theory (DFT) is used to probe the structures of triruthenium clusters Ru3(CO)12. The researcher shed light on the equilibrium geometries and the best function (PBE1PBE) and basis set (SDD Ru/ SVP on (C, O)) can be used. On the basis of that, the global minimum found for Ru3(CO)12 is an unbridged (1D3) structure. In Chapter 3, DFT with PBE1PBE functional and (SDD Ru/ IGLOIII, TZVP P, H/ SVP on (C, O)) basis sets have been used to identify structures of species observed in hydrogenation reactions of alkynes using catalysts based on Ru3 clusters. The identification of the hydride intermediates observed by NMR spectroscopy is ambiguous, and is generally based only on 1H NMR and JP - H coupling constants for hydrides attached to the ruthenium metal centres. Our calculations propose that the experimentally observed species A and B do not contain the Ru3(? - H)(H) motif, as proposed by Duckett group but have Ru3(? - H)2. In Chapter 4, these studies were extended to the analogous dihydride triruthenium monodentate and bidentate phosphines. By computing energies, 1H chemical shifts, we propose that the experimentally observed species A1 and B1 do not contain the Ru(µ - H)(H) motif, as proposed by Duckett and co - workers, but rather the dibridging Ru(µ - H)2 structures common to A2 and B2. In addition, the results confirm that our methodology is able to model the environment of a terminal hydride correctly.Finally, Chapter 5 describes the Quantum Aim (QTAIM) topological analysis of the electron density in the Picolyl N - Heterocyclic Carbene Triruthenium cluster [Ru3(? - H)2(?3 - ?3C2,NHCpyCH2ImMe)(CO)8] (3 - methylimidazol - 2 - ylidene). However, this cluster has been chosen since, firstly, has Ru3(? - H)2 motif similar to triruthenium core proposed in chapters 3 and 4 , secondly, this particular cluster contains one hydride - unbridged and two hydride bridged Ru - Ru edges and a face - capping ligands with different types of Ru - C and Ru - N bonds , this allowing interesting comparisons between the topological properties of related but different atom - atom interactions, within the same molecule.

العلاقة بين المشعرات المهبلية والعوامل المرضية التناسلية الاخرى بين النساء في مدينتي كركوك وتكريت Relationship Betwee Trichomonas Vaginalis And Other Genital Infectious Agents Among Women In Kirkuk & Tikrit Cities

اسم المؤلف: برهان احمد محمد علي بيباني
اسم المشرف: يحيى جرجيس سلمان ابراهيم شعبان داود
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم خلال الفترة من الخامس عشر من شهر تشرين الاول 2006 ولغاية الثلاثين من شهر حزيران 2007 اجراء دراسة عن وبائية طفيلي المشعرات المهبلية وبعض مسببات الالتهابات المهبلية الافرازية المرافقة وغير المرافقة لها بين النساء المراجعات لقسم الاستشارية النسائية والتول | An epidemiological study was carried out from 15th, October 2006 to 30th, June 2007 for prevalence of Trichomonas vaginalis parasite and some causative agents of secretary vaginal inflammations associated or non - associated with the parasite among women attending the department of Obstetrics and Gynecology in two General hospitals in Kirkuk city and Tikrit teaching hospital in Tikrit city. A total of 300 samples (200 in Kirkuk, 100 in Tikrit) were colle - cted from (15 - 47) years old women. Two swabs were taken from the vaginal (high vaginal) and the lining of uterus cervix (endocervical) for each woman by Gynecologic physician. A gross examination of the swabs was done to determine the color of vaginal secretions and measurement of pH. A wet preparation was done from the (high vaginal swab) and examined microscopically for diagnosis of Trichomonas vaginalis and Candida spp. In addition a Gram stain smear was prepared from a portion of the same swab and examined by oil - immersion for diagnosis of Neisseria gonorrhoeae and Trichomonas vaginalis. A chemical test was done for the detection of Gardnerella vaginalis bacteria & Candida spores. The swabs prepared from endocervical were treated by ACON - Chlamydia Kit specific for diagnosis of Chlamydia trachomatis in vaginal swabs samples. The study concluded the following results : 1 - The infection with various sexually transmitted diseases agents were 68% , 78% in Kirkuk & Tikrit cities respectively (P>0.05).2 - The maximum infection rate for T. vaginalis was 19.5% in Kirkuk city & 16% in Tikrit city among co - infection with other agents and single infections.3 - The percentages of single infections with T. vaginalis alone for various age groups were 16.5%, 8% in Kirkuk and Tikrit cities respectively (P>0.05).4 - The rate of infections with various vaginal micro - organisms were in the following sequence : T. vaginalis (19.5%), G. vaginalis (18.5%), C. trachomatis (14%), Candida spp. (11.5%) & N. gonorrhoeae (4.5%) in Kirkuk city, whereasin Tikrit city the sequence of infection rates were as follows : G. vaginalis (43%), T. vaginalis (16%), C. trachomatis (12%), Candida spp. (6%) & N. gonorrhoeae (1%).(P>0.05).5 - The highest rates of infections with T. vaginalis only as a single infecting agents were 21.27%, 10% among a group age range (36 - 45) years in both Kirkuk & Tikrit cities respectively (P<0.05). 6 - The maximum rate of infection with the T. vaginalis were recorded among women with yellowish vaginal secretions without itching (37%), (31.25%), whereas in those with symptoms of copious secretions onlythe corresponding figures (30.17%), (18.51%) were recorded in bothcities respectively (P<0.05). 7 - The maximum infection rate with C. trachomatis in bloody swabs were (40%), (50%) in Kirkuk & Tikrit cities respectively correlated withclinical signs & symptoms. However, maximum infection rate with G. vaginalis was recorded in women with whitish vaginal discharge (60.86%) or yellowish (53.12%) then greenish (52.94%) in Tikrit city in comparison to Kirkuk city. The maximum infection with G. vaginalis were among women with bloody secretion (40%) followed by whitish secretion (30.35%). (P<0.05). 8 - The maximum infection rate with Candida spp. were recorded in women discharge milky secretions (33.33%), (25%) in Kirkuk & Tikrit cities respectively. Infection with N. gonorrhoeae showed different color vaginal discharge (P>0.05).9 - The maximum rate of infections with T. vaginalis (27.43%), (19.69%) were recorded in women whose pH of vaginal secretion range was between (5 - 6). (P> 0.05). The high vaginal swabs have been revealed to be more efficient in diagnosis of vaginal infection with T. vaginalis than endocervical swabs. The maximum rate of infections recorded by these swabs were (22.22%), (19.73%) respectively. The infection rate of pregnant women were (48%), (22.22%) from positive cases in both cities.10 - The maximum rate of infections with T. vaginalis were recorded in women using various contraceptive, women using intrauterine contraceptive device were the highly infected (33.33%), (31.57%) in both cities. P<0.05

تقييم دور الجين في دم نساء عراقيات مصابات بسرطان الثدي Evaluation The Rule of Mammoglobin A Gene In Blood of Iraqi Women With Breast Cancer

اسم المؤلف: نانسي فيصل رشيد
اسم المشرف: امنة نعمة الثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Mammaglobin A is a member of the superfamily of secretoglobins. Its expression is highly specific for mammary tissue and has been shown to be overexpressed in breast tumor tissue, indicating that mammaglobin A might confer a growth advantage to mammaglobin A - expressing tumor cells. The present study was undertaken to develop the mammaglobin A as a serum biomarker for the early detection of breast cancer, and breast cancer patients follow up after treatments. This study had taken ten months, it involved 84 patients with age ranged 15 - 74 years who had diagnosed with either stage I to III breast cancer or benign lesions of breast at certain Iraqi hospitals and breast cancer centers, and a total number of 10 apparently healthy individuals, with comparable age range of patients as control group.Ten ml of venous blood was collected from studied groups in order to be used for measuring estrogen and progesterone levels, Reverse transcription polymerase chain reaction (RT - PCR) test for detection of their serum m - RNA mammaglobin A existence, and serum mammaglobin A levels using Enzyme - Linked ImmunoSorbent Assay (ELISA) technique. Demographic study revealed that most cases enrolled in this study were women within their reproductive age 25 - 44 years old. Distribution of patients according to age group, marital status, and lactation status showed no statistically significant association (P>0.05) with any of these variables, in all comparison.The hormonal study states that elevated levels of estrogen were observed in 52 (62%) patients, also decrease in progesterone levels were observed in 50 (59%) patients out of the 84 cases studied. This indicate a highly significant SUMMARYassociation (p < 0.01) between breast cancer risk, elevated estrogen levels, and decrease in progesterone levels. Mammaglobin A protein was detectable by ELISA in 100% of breast cancer patients (65 case), and not in any benign tumor patients (19 case) nor healthycontrol women (10 females) which indicate the highly specificity of ELISA test (P<0.01) for measuring mammaglobin A levels in patients serum. With high concentration of protein in sera of women with late stage of breastcancer compared with a low protein concentration in sera of women with early stage of breast cancer. By applying a statistical analysis on the result it was found that serum mammaglobin A ELISA test is highly significant (P<0.01) for, differentiation between patients with metastatic breast cancer and patients with early diagnosed breast cancer. In this work, SCGB2A2 gene which coded for mammaglobin A was detected in 64 out of 65 breast cancer cases, but not in the benign or healthy individuals indicating its high specificity as a marker gene (SCGB2A2) for cells derived from mammary glands. A statistical analysis was made for comparison between the three groups, E - Malignant Tumors \ Early Stage, L - Malignant Tumors \ Late Stage, and B - benign, (E, L, and B) which show that the results of molecular study of mammaglobin A detected by RT - PCR is statistically high significance (P<0.001) when used for the differentiation between E group and B group, also between L group and B group. The results showed that mammaglobin A can become an important tool for detecting primary and metastatic breast cancer, and predicting disease outcome.

دراسـة بيئيــة للافقريات القاعيـة في هور الدلمج - وسط العراق An Ecological Study of The Benthic Invertebrates In Al Dalmage Marsh - Middle of Iraq

اسم المؤلف: ميسون حسن مشجل السراي
اسم المشرف: هيفاء جواد جوير
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية التنوع الاحيائي لمجتمع لافقريات القاع في هور الدلمج / وسط العراق فضلا عن بعض الخصائص الفيزياوية والكيمياوية لمياه الهور، اختيرت ست محطات لجمع العينات وذلك للمدة من بداية كانون الثاني وحتى كانون الاول 2013. استخدم تحليل التوافق CCA | Benthic invertebrate diversity on the Al - Dalmage marsh in the middle of Iraq, in addition to the some physical and chemical properties was the goal of the present study, for a period from January to the end of December 2013. Six stations along the eastern side of the marsh were chosen for monthly samples collection.The results showed water temperature ranged from(9 - 34.1)?C. The pH values were neutral but tends to near alkaline and ranged from (7.2 - 9).The Electrical conductivity values varied from (22735 - 2330) ?s/cm, according to the values of salinity (1.5 - 14.6) ‰ the marsh was classified as mesohaline (TDS) which showed high values ranged from (1820 - 17762) mg/L.This study showed that the marsh were well oxygenated due to the dissolved oxygen values which ranged from 7 - 12 mg/L, the study recorded values to BOD5 were ranging from (1.5 - 3)mg/L, and the marsh classified as very hard according to the total hardness values from (6980 - 825) CaCo3 mg/L. The high perecentage of organic matter in the sediment was 2.7 %. The results showed the station were characterized by sediment texture varied from silt clay sand sediment in the stations 1, 4, 5 , silt clay in St(2) and sand silt in St(3), sand clay silt in St(6).A total of 21286 individual benthic invertebrates was sorted, which represents which 78 taxonomic units, including 9 taxa. The Crustacea was the dominate group representing 29% of the total number of benthic invertebrates. It was noted that there were obvious differences in the total number of sorted individuals in which varied in each location and each month. The highest total number recorded in was st (2) was 4360 including 73 taxonomic units, while the highest total number recorded in April was 2624.The results of The relative abundance index showed that the species insects : Polypedilum sp Were more abundant in St(2 - 6), and dominant in St(6), while the Seinura sp were dominant in St(6), while the other taxonomy units varied from less abundant to rare abundant. Also the results of constancy index showed The which were considered "constant " However the other taxonomy units varied from "accessory " and" accidental" taxonomy units in study stations.The Shanon - Weiner index of totall benthic invertebrates varied from (1.2 - 3.8) bit/Ind, The uniformity index of benthic invertebrates varied from(0.7 - 1), these high values indicate that there is no ecological stress on benthic invertebrates in the study area.The Results of Jaccard Presence - community of similarity showed the highest degree of similarity to be 96% which recorded between St(1) and St(2).The values of the index of spcies richness varied from 1.3 - 15 with the greatest values recorded in April while lower values were in October.Six species of Turbellarian Platyhelminthes that were recorded, from which Stenostomum leucops, Stenostomum bryophilum, Stenostomum unicolor, Gyratrix hermaphrodites, Macrostomum sp, Dalyelliidae, which were considered as new records for Iraq, with Gyratrix hermaphrodites as the most abundant species and representing 25% of the total number of Turbellarian. It was noted that there were obvious differences in the total number of sorted individuals which varied in each location and each month, the highest total number recorded in St 3 was 400, while the highest total number recorded in November was (198).The present study result showed that three species of Nematoda were recorded incuding Seinura sp, Alaimus sp, Dorylaimus sp, Seinura sp as the most abundent species representing 84% of the total number of Nematoda. Spatially the highest total number of 590 individuals was recorded in St (4) , while temporally, the highest total number of 366 was recorded in October.One unidentified species of Gastrotricha was recorded for the first time in Iraq, 102 Ind were sorted these were considered "constant" recording to constancy index 63.8% which were sorted from all stations.A total of 2319 individuals of Rotifera was sorted, these were identified as 15 species that included Philodina sp, Rotaria citrinus, Dipleuchlanis sp, Euchlanis sp, Notholca sp, Ttriploeuchlainis sp, Colurella sp, Lepadella sp, Lecane sp, Proales sp, Cephalodela sp, Synchaeta sp, Hexarthra mira, and finally Brachionus sp which was recorded the highest number of individuals 540 individuals.A total of 1888 individual Annelida were sorted representing 12 species including nine species belonging to the family Naididae (three belong to the sub family Naidinae and six belonging to Tubificoid worms), two species belonged to Aleosomatidae including Aeolosoma hemprichi, Aeolosoma variegatum, and finaly one specie belong to Hirudinea which was Glossiphonia heteroclite, with three 3 ind in all study.The family Aleosomatidae recorded the highest percentage of 58% of the total number of Annelida.The sub family Naidinae including Paranais litoralis, Dero (Aulophorus) furcata, Stylaria lactustris.The highest frequency of 23.6% was recorded by P. litoralis.The Tubificoid worms represent six species which were Limnodrlius hoffmeisteri, Limnodrlius profundicola, L. claparedeianus, L udekemianus, Tubifex tubifex, Branchiura sowerbyi. This study also isolated 6238 individual Crustacea, these were identified as 15 species that included five species belonged to Cladocera, four order belong to copepod, which were recorded the highest percentage 30% of the total number of Crustacea, in addition to Macrobrachium nipponense from Decapoda, Sphaeroma annadalei anndalei from Isopoda, Ostracoda, Amphipoda, Conchostracha. It was noted that there was obvious differences in the total number of sorted individuals which varied in each location and each month, The highest total number recorded in st (2) was 1480, while the highest total number of 730 was recorded in April. The study finds 4297 individual insects groups, were sorted represent seven order includes larvae of Ephemeroptera, Trichoptera, Odonata, (Zygoptera, Anisoptera) Diptera (Culicidae, Chironomus sp, Polypedilum sp) Lepidoptera larvae Hemiptera (Corixidae, Nepa cinerea), and adult of Coleoptera (Dysitidae, Hydrophilidae Elmidae, Curculionidae, Coleoptera larvae), the Diptera larvae were recorded the highest percentage 55 % of the total number of insects groups, and Nepa cinerea was considered as new records for Iraq.This study showed two class from Mollusca, 1956 Ind were sorted, including Gastropoda and Pelecyopoda.The Gastropoda which were recorded the highest percentage 94% of the total number of Mollusca, these were identified to physa acuta, Lymnaea sp, Melonoides tuberculata, Melnopsis nodosa, Theodoxus sp, physa acuta which was the greatest percentage 41% of the total number of gastropoda. pelecyopoda which were including Pseudodontopsis euphraticus, Tigridis Unio, Corbicula fluminea, Corbicula fluminali

دراسة تصنيفية حياتية مقارنة لانواع من الجنس Euphorbia L. (Euphorbiaceae) في العراق A Comparative Biosystematic Study of Some Species of The Genus Euphorbia L. (Euphorbiaceae) In Iraq

اسم المؤلف: ازهار طاهر صليبي
اسم المشرف: عذية ناهي سلمان المشهداني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تصنيفية مقارنة لثلاث وعشرين مرتبة تصنيفية تعود جميعها للجنس Euphorbia L. من العائلةEuphorbiaceae النامية في العراق، تضمنت الجوانب المظهرية العامة والدقيقة ودراسة حبوب اللقاح والمركبات الكيميائية والبيئة والتوزيع الجغرافي فضلا عن ا | The current research deal with twenty - three taxa belong to the genus Euphorbia L., Family Euphorbiaceae grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also studied and the chemical compounds, Ecological and geographical distribution and taxonomic treatment have done.Also botanical key was putted to separate the species. General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens studied, and correct the mistake naming, specimens were identified and reidentified some miss identified specimens in the Iraqi herbaria. A comparative morphological study was undertaken to all plants parts of taxa appeared from habit and duration of taxa to the quantitative and qualitative attributes the members of the vegetative and floral parts included the roots, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying with natural images for different morphological characteristics, as well as the field images. This study showed that all species was herbs, shrubs and succulent and semisucculent annual and perinnal, the major taxonomic importance was for flowering parts includeing the specific Inflorescence, installation of a cupped and its regularity on the branches of the plant, glands forms, numbers, presence or lack of appendages, bractes and bracteoles shapes, dimensions and color fruits shape, indumentum and seeds forms, dimension and her surface sculpturing; and the presence or lack of caruncle, as well as the qualities of roots, stems and leaves, and the morphological characteristics of all plant parts mentioned above have a taxonomic importance in identification and separation of the studied taxa. The study of the micromorphological characters of hairs, leaves epidermal cells and the stomatal complex which included anomocytic, paracytic, anisocytic and tetracytic, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa.The pollen grains have a taxonomic importance because their different in shape, dimensions of polar and equatorial view, the wall thickness and their ornamentation. They are mostly of Tricolporate except two species were Hexacolporate, also the differences in their shape in the polar and equatorial view.They were spheroidal, ovoid and oblate - spheroidal in the polar view and oblate, spheroidal, subprolate and oblate - spheroidal in the equatorial view.The ornomentation they were reticulate, microreticulate and foveoate.The chemical study also importance in separation of the genus taxa four compounds Flavonoids diagnosed in all the studing species but different in concentrations, by using HPLC, they are Myriscetin, Quercetin, Kaempferol and Coumarin esculetin and this confirms that there are a common evolutionary link between the species in terms of their chemical properties, which suggests belonging species to the genus Euphorbia.Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical districts. the species of Euphorbia were distributed in all Iraqi districts especially in LCA, MSU, MRO, and DWD and some species were farmed in gardens and nurseries either to the beauty of their bractes colors or because their acceleration to the environmental changes such as succulent species, As well as wild species were spread in mountainous areas, oak, nuts forests, in orchards, cultivated fields, flat plains and desert areas in loam soils, clay, gravel, and sand. Through this study some new taxa where founded in Iraq and they are E. cyparissias Kunth ; E. maculata L. ; E. serpens L. ; E. tirucalli L., and this study suggests to recorded them for the first time in Iraq.

دراسة بيئية وسكانية للقوقع الارضي Candidula gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) في مواقع مختارة من محافظة بغداد Ecological And Population Study of The Land Snail Candidula Gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) In Selected Locations From Baghdad Province

اسم المؤلف: بشرى محمد كاظم ديوان البيضاني
اسم المشرف: عماد الدين عبد الهادي المختار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Few studies on terrestrial snails in general, and garden snails in particular, existin Iraq. The group being important apparently for its wide - spread distribution in various habitats. One species of this group which has been previously recorded in Baghdad, is the garden snail Candidula gigaxii, class : Pulmonata, family : Hygromiidae.Available samples of the land snail C.gigaxii were collected from four locations within Baghdad province; a Taji, the Army Canal area, the Zafaraniyah, and Al - Jadiriya district, the study period of June 2013 to May of 2014.Environmental measurements were recordedat each site. Results showed that air temperature range was 10 - 34? C, soil temperature range was 5 - 26? C, pH range of the soil was between 4 - 8.9 and the content of the soil organic matter was between 0.3 - 2.0%.Snail samples were divided into seven size - classes according to the dimension of the shell and these were <2, 2 - 4, 4 - 6, 6 - 8, 8 - 10, 10 - 12, >12 mmPercentages of each size class were calculated. Having measured shell dimensions, shell growth index (Ia) was also calculated. The range of growth index value for this snail was 1.9 - 3.86 where the highest value was recorded at the Taji site in January 2014 which was probably attributed to low temperature range and high soil moisture and organic matter content.Results also showed that C. gigaxii favored soils with moderately high pH values and rich in organic content. Soil moisture appeared to be a limiting factor for growth; and a loamy soil texture for existence of the studied species. Average highest population density of living snails and empty shells were 44 and 32 individuals / m2 respectively. The overall population density was also calculated for the purpose of obtaining realistic population density of the species.

دراسة نوعية مياه خزان الحبانية موقعيا والاستشعار عن بعد Study of The Water Quality In Alhabbaniyah Reservoir In Situ And Remote Sensing

اسم المؤلف: احمد عطا الله حسن الفهداوي
اسم المشرف: عادل مشعان ربيع
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

العلاقة بين مؤشر الموت الخلـوي المبرمج (ربيطة فاس الذائبة) وقلة النطاف عند الرجال Correlation Between Apoptotic Marker (Sfas Ligand) And Oligozoospermia In Men

اسم المؤلف: احمد حسين علي الزاملي
اسم المشرف: صباح ناصر العلوجي انعم رشيد الصالحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The Fibroblast associated (Fas) system in the testes has been identified as a key regulator of apoptosis, a process that greatly influences the germ cell population of the testes. and the soluble Fas Ligand (sFasL) may be a competitive inhibitor to induce apopto¬sis of the Fas baring cells. Therefore, this study measures the level of sFasL in the semen of oligozoospermic men evaluating the association between seminal plasma sFasL and spermatogenesis.A total 58 oligozoospermic men and 29 normozoospermic volunteers were included in this study. They were attending the Infertility Clinic of the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, AL - Nahrain University\ Baghdad. Their age mean was 31.77± 5.48 years and a median of 30 years. Semen samples were evaluated according to World Health Organization 2010 standard parameters. The Seminal plasma was separated from the cellular elements after centrifugation and the supernatant was transferred to plane tubes and frozen at - 20 ?C till using for measurement of sFasL. These patients were classified into patients with severe oligozoospermia (sperm concentration ?5 million / ml) which include 29 patients, and patients with mild and moderate oligozoospermia (sperm concentration ranging between 5 - 15 million / ml) which includes 29 patients as well. The control group comprised of 29 men with normospermic parameters according to WHO 2010 standard (Normal volunteers). The sFasL was measured using ELISA enzyme immunoassay for quantitative determination of sFasL Kit. Data were analyzed statistically using Analysis of variance (ANOVA), Least Significant Difference (LSD) and Correlation Coefficient (r).The sFasL level was found significantly higher (P< 0.01) in seminal plasma of oligozoospermic men, with weak correlation of the level of sFasL with the degree of severity of oligozoospermia. Based on the results of this study, it was concluded that sFasL is a novel marker found in the seminal plasma of oligozoospermic men. It's level was higher in cases of oligozoospermia regardless of its severity.

تكامل جرعة منخفضة من مبيد الترفلان مع مخلفات زهرة الشمس في مكافحة الادغال وتحسين نمو محصول الماش لتقليل الاعتماد على المبيدات والحد من مخاطرها Integration of Reduced Dose of Trifluralin Herbicide With Sunflower Residues For Weed Control In Mungbean Field

اسم المؤلف: اﺭﻭﻯ ﻋﺒﺪ ﺍﻟﻜﺮﻳﻢ ﺗﻮﻓﻴﻖ
اسم المشرف: اﺑﺮﺍﻫﻴﻢ ﺷﻌﺒﺎﻥ ﺍﻟﺴﻌﺪﺍﻭﻱ
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments, several greenhouse and laboratory experiments were conducted during the course of study to evaluate the allelopathic potential of two sunflower (Helianthus annuus L.) cultivars on companion weeds and weeds grown in mungbean (Vigna radiata L. R. Wilczek) field alone and in combination with reduced rate herbicide, and to determine the chemical and genetical bases of allelopathic traits in the test cultivars. The aim of the first experiment was to test whether the variation in weed growth between the sunflower cultivars (Shumoose and Sin Altheeb), which was observed in the field, was due to differences in their allelopathic potential. Results showed the ability of both cultivars to reduce weed population and biomass with the superiority of Shumoose cultivar in suppression of weed density at 60 and 120 days after sowing compared to Sin Altheeb. Weed biomass was significantly reduced up to 90 and 71% by Shumoose and Sin Altheeb cultivars, respectively. Stair case experiment indicated that root exudates of Shumoose cultivar showed more suppression to weeds than Sin Altheeb giving additional evidence for the superiority of Shumoose cultivar in its allelopathic weed suppression. Chemical analysis by High performance liquid chromatography indicated the presence of 9 compounds in root exudates of Sin Altheeb and 8 compounds in Shumoose, and all are Phenolic in nature. However, total of the isolated phenolics was doubled in Shumoose than in Sin Altheeb. Most of the isolated chemicals are reported to have inhibiting ability for germination and growth of plants, including weeds. Second field experiment was undertaken to explore the response of weeds and mungbean crop to soil incorporated with sunflower residues in combination with lower rate of a pre - plant herbicide (trifluralin). Sunflower residues of the two cultivars Shumoose and Sin Altheeb at 3.2 and 6.4 t ha - 1 were used either alone or in combination with trifluralin at 1.2 L ha - 1 (50% of label rate). Weedy check (control), trifluralin at full label rate (2.4 L ha - 1), and weed free treatments were also included for comparison. Plots treated with 50% of label rate of herbicide and amended with sunflower residues of cultivar Sin Altheeb recorded least weed density and dry biomass and this suppression was much greater than the residue treatments alone and more than cultivar Shumoose. Application of herbicide at 50% rate in plots amended with Sin Altheeb residue resulted yield 64 % more than with the label rate of herbicide treatment. Chromatographic analysis of sunflower amended field soil revealed the presence of several potent allelopathic compounds in the residues of both cultivars with greater quantity (355.5 µg/ml) in Sin Altheeb than in Shumoose (250.9 µg/ml). Dynamics of release, decomposition and degradation of allelochemicals into the soil showed that different compounds exhibited differential behavior for these processes. Overall quantity of allelochemicals started to increase after 2 weeks of decomposition and peaked at 4 week of decomposition (180.1 ppm) then declined sharply in their quantities thereafter. Periods indicating higher quantities of total phenolics as shown by chromatographic analysis was coincided with the periods in which higher suppressive activity against weeds grown under field conditions. Bioassay of sunflower residues decomposed in soil at different times on Echinochloa colonum L, one of the weeds dominated the mungbean field, revealed that residues of both cultivars suppressed growth of E. colonum weed. The phytotoxicity started after 2 weeks and persisted for 8 weeks. However, Sin Altheeb residues showed greater inhibition than Shumoose cultivar at the last three decomposition periods. Shumoose residues recorded appreciable reduction to the growth of test weed compared to Sin Altheeb. The effect of residues of both cultivars on chlorophyll content and ions uptake in Amaranthus retroflexus L. weed revealed that residues of both species reduced chlorophyll content of leaves and the reduction increased with the increased residue rate. Sin Altheeb cultivar residues were more inhibitory than Shumoose at the lower residue rate. In the same weed, ions uptake was significantly averted by the residues of both cultivars. P and K was significantly increased over control, while Ca, Mg an Fe were found to be decreased over control due to application of sunflower residues in soil at 8 g per kg soil. N was the only test element that did not significantly influence by the residue treatments. No significant differences were found in the test ion uptake between the test sunflower cultivars. Results of using RAPD technique on the sunflower genomic DNA revealed that all the 19 primers used in this study scored different amplification monomorphic and polymorphic bands in the tested genotypes with 12 primers generated polymorphic bands. These bands present in one cultivar and absent in another. They could be responsible for allelopathic trait or secondary compounds associated with this phenomenon. Further work is recommended to analyze the sequence of this band to find out whether it is related to allelopathic trait or not.

التنميط الجيني لفايروس التهاب الكبد نوع اي لمرضى الاصابة الحادة في محافظة المثنى - العراق Genotyping of Hepatitis E Virus Among Patients With Acute Infection In Al - Muthanna Province / Iraq

اسم المؤلف: ازهار صبري مسلم الذهبي
اسم المشرف: رغد حربي مهدي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Hepatitis E Virus (HEV) infection is found worldwide. It is responsible for large outbreaks in East and South Asia. This study was carried out to identify the distribution of acute HEV infection in AlMuthanna province and then determine the genotypes responsible for the disease spreading. This is the first investigation about the occurrence of hepatitis E genotypes in Al - Muthanna province patients. The study included 270 jaundiced patients of both sexes From AlMuthanna province which included out patients in public health laboratory in Samawah city, for the period from October 2013 to June 2014. All these patients were tested for anti - HEV IgM antibodies by using enzyme linked immunosorbent assay (ELISA). In Al - Muthanna province, 72 patients (26.66 %) out of 270 patients showed positive results for anti - HEV IgM antibodies, and all those patients were tested for confirmatory test at central public health laboratories (CPHL) in Baghdad province. Those patients consist of 45 females and 27 males with age ranged between (4 - 74) years old, were all negative for routinely screened markers of Hepatitis A, Hepatitis B and Hepatitis C, Ten normal healthy individuals, used as normal control in this study. This study showed that the HEV IgM is more common among younger age group (15 - 24), with a percentage of) 41.67%(, and it was higher in females (63%) than in males (37%). Highly significant differences (p ? 0.01) appeared among age groups. The levels of liver function enzymes demonstrated significant differences (p ? 0.05) in HEV patients as compared with healthy controls. The levels of Total Serum Bilirubin (TSB) were (23.41 ± 12.38) mg/dl, Serum Aspartate transaminase (SAST) were (887.17 ± 9.87) U/l, Serum Alanine transaminase (SALT) were (859.91 ± 13.28) U/l, and the levels of Serum Alkaline phosphatase (SALP) were (206.56 ± 5.04) U/l higher than healthy controls (0.884 ± 0.05) mg/dl, (37.27 ± 4.58) U/l, (34.14 ± 7.63) U/l, (48.09 ± 15.42) U/l respectively. RNA was extracted from sera of positive anti - HEV IgM antibodies by using a QIAamp® Viral RNA Mini Kit. Nanodrop and Quantus™ Fluorometer instruments were used to determine the RNA yield and purity according to the standard kit protocol. High levels of RNA concentration were measured by Nanodrop and Quantus™ Fluorometer. Forty - six samples have high RNA concentration out of 72 samples were detected for genotypes I, III & IV by using Applied Biosystems Real time PCR 7500 machine. In this study, HEV type I and III were detected in 23 samples out of 46 samples by using Real Time PCR systems. Ten samples were positive for this test. Thirteen samples (56.52%) were negative for this test. The distribution percentage of genotypes I and III was (43.48%). The results appeared significant difference (P ? 0.05). Also by using the same technique (Real time PCR systems) the HEV type IV was detected in 23 samples out of 46 samples. Which included, 13 samples that recorded negative results by I & III genotypes kit and only 2 samples from it recorded positive results and 11 samples recorded negative result for HEV genotype IV. From other 10 samples, only 3 samples recorded positive result and 7 samples recorded negative result for HEV genotype IV. In a total, 5 samples were positive for HEV genotype IV and 18 samples (78.26%) were negative. So that the distribution percentage of genotype IV was (21.74%). In this study, the samples that showed negative results in both kits have not been tested for other genotypes. This study indicated that the acute HEV infection is highly endemic in Al - Muthanna province in Iraq. Genotypes I & III were the most distribution than genotype IV in patients from Al - Muthanna province in Iraq. These results suggest that the genotypes I & III are the main causative agents of sporadic HEV infection in Al - Muthanna province

تاثير بعض العوامل البيئية في مجتمع الهائمات القشرية لنهر دجلة وجزيرة بغداد السياحية - العراق The Effect of Some Environmental Factors In Crustaceans Plankton Community of The Tigris River And The Island of Baghdad Tourist - Iraq

اسم المؤلف: اعتدال فليح حسن العامري
اسم المشرف: صباح فرج عبد الاحد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study was conducted to investigate the effect of the water body type on the density of crustaceans zooplankton of the cortical Cladocera and two orders of copepod (Cyclopoida, Calanoida), addition to the physical and chemical effect of lake of Baghdad Tourist Island and Tigris river within the water of the lake near the site and electric and know the effect these characterties on density of crustaceans Zooplankton and for doing this study. We choose five places, tow on Tigris river and three places on the Island in AI - Fahhama in north of Baghdad The samples of water were collected every 15 days all these stations from beginning of December 2013 to May 2014. This study had corrected the temperature of air and water and the pH, electrical conductivity. and Dissolved oxygen, BOD5, NO?¯, PO?¯ and the density of Crastaceans Zooplankton. This study showed that the temperature of the air was nearly in the river and the lake and that because of their being in the same geographs area.The temperature was between (16°c - 34°c)in Tigris while it was (11.9°c - 33°c)in the lake Hydrogen Ions, it was (7.3 - 7.9) in the river and (8.18.8)in the lake because that were a lot of water plants and phytoplankton. This study shows that water of the river and the lake in the areas of study have a good of air and that was shown by recording the heigh values of oxygen. The values of dissolved oxygen (DO) was between (8.11 - 11.5) mg\L in river between (7.9111.37)mg\L in lake and showed from the study that the values were few. It was less than 5mg\L it was (0.33 - 2.6) mg\Lin the river and (0.64 - 3.55) mg\L in the lake. The values electrical conductivity of water in all the placed the study in the permissible range the water, it isn't over than 1500?s\cm. The electrical conductively records were between (851 - 1184) ?s\cm in the river, and it was (755 1270) ?s\cm in the lake. From the other hand nutrients, the phosphor records between (0.025 - 0.0563)mg\Lin the river and in the lake between(0.00310.0429)mg\L The Nitrate records its values highs in the month of winter because of the rain and it was increased in the river, it was (0.345 - 0.9135)mg\L compare with the lake, it was (0.028 - 0.626)mg\L in the places of the study that were appearance of two clear increases in the density of Zooplankton one was in winter and the other was spring.The density of zooplankton in the lake much more than it was in the river. The density of the zooplankton belong to Cladocera between (0.019 - 0.307) ind.\L in the lake and the density of the Calnoida was between (0.003 - 0.065)ind. \L in the river and (0.0078 - 3.37) ind. \L in the lake. The results shows differences if there are changes in places and month on most of physical and chemical biological characteristic the measured during the period of this study. The kind of water body effects on most characteristics and soon the density of crustaceans zooplankton.the results of the study mention the binding morale relation between the density of zooplankton the values of electrical conductivity and value of BOD(Biological Oxygen Demand) while there isn't any morale relation between the density of zooplankton and the value of Hydrogen Ion and Nitrate and dissolved oxygen that because the value of these changes was near to be equal in all the places that eayses the reduce of its effective on the average of density of these organism. The study shows that Baghdad lake have the environmental ingredients makes the lake natural protected to forming and breeding a fish in the lakes in addition that it is a place for Tourism.

تاثير عصارة نبات البروكلي في متلازمة تعدد الاكياس المبيضية المستحدثة في الجرذ الابيض Effect of Broccoli Plant Juice On Induced Polycystic Ovarian Syndrome In Albino Rats

اسم المؤلف: رقية احمد صالح
اسم المشرف: نهلة جاسم محمد الشاهري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The goal of present study was to investigate the benefits of broccoli as anti androgenic plant in protection and treatment for reproductive hormonal and metabolic disturbance, which combined with polycystic ovarian syndrome (PCOS). Three experiments for 3 PCOS models were done. First Experiment : The prepubertal female rats at 21 days old, were injected with Testosterone (T) subcutaneously for 39 days (first model). The females of this experiment were divided into 6 groups (8 rats each) as follows : Group 1 was injected with sesame oil. Group 2 was injected with T hormone. Group 3 was injected with T hormone with orally gavaged of broccoli juice. Group 4 was injected with sesame oil, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 5 was injected with T hormone, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 6 was injected with T hormone, and at the end of the last injection was orally gavaged with broccoli juice for 30 days. Second Experiment : The prepubertal female rats at 21 days old, were injected with Letrozole (L) subcutaneously for 39 days (second model).The females of this experiment were divided into 6 groups with the same style of the first experiment. Third Experiment : it was achieved by the injection of pregnant rats prenatally (days 16 to 19 of gestation) with 1 mg of T hormone subcutaneously (third model), and then the female offspring were divided into groups (8 rats each) as follow : The first offspring group : The female rats offspring at 21 days old (their mothers were injected with sesame oil from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with distilled water : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with broccoli juice : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with broccoli juice until 60 days of age. The third offspring group : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation and orally gavaged with broccoli juice) were orally gavaged with distilled water until 60 days of age. Females of all groups were sacrificed after 24 hours from the last treatment to undertake weight, histological, hormonal and metabolic study for each group. the results of present study showed the symptoms that reported PCOS of the three female experiments such as ovarian cyst, hyperandrogenemia, hormonal and metabolic disturbance, etc. Also a well improvement has been noticed in all syndrome symptoms on females gavaged with broccoli juice for all three rats PCOS experiments, these results indicate that broccoli juice has got a protective and curative role against this syndrome.

التحري الجزيئي عن النمط الثالث لداء السكري البادئ عند النضج (MODY 3) في مرضى السكري العراقيين Molecular Investigation of Maturity Onset Diabetes of The Young Type 3 (MODY 3) In Iraqi Diabetic Patients

اسم المؤلف: اسراء عدنان ابراهيم البغدادي
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شملت الدراسة 63 مريضا مصابا بداء السكري غير المناعي المنشا في اعمار مبكرة من ذوي التاريخ العائلي بالاصابة بمرض السكري ممن لايعانون البدانة الذين تم اختيارهم من مرضى السكري من مراجعي المركز التخصصي لامراض الغدد الصم والسكري في الرصافة/ بغداد للفترة من ايار | This study included 63 non obese patients having early onset of non immunogenic diabetes with strong family history of diabetes selected from diabetic patients attended the specialized center of endocrinology and diabetes at Alrusafa/ Baghdad during period from the begging of May 2011 till the end of February 2013. The demographic study revealed that there is no association between the disease incidence with neither the gender nor the blood group. But there is a significant difference in the distribution of the patients according to age of the first diagnosis and gender, as the study showed that majority of male patients (53.846%) were first diagnosed with hyperglycemia below the age of 20 while the majority of females (72.972%) were first diagnosed with hyperglycemia after the age of 20.The distribution of patients according to the type of treatment also reveled association between the gender and both age of diagnosis and type of treatment of the hyperglycemia as the study showed that there is a higher percent of male patients (80.768%) using insulin at the beginning of their diagnosis with hyperglycemia or transferred later to insulin than those of females (67.565%).The present study reported for the first time in Iraq the presence of monogenic diabetes (maturity onset diabetes of the young type 3) as major cause of diabetes within non obese diabetic patients' early onset of non immunogenic diabetes with strong family history of diabetes. The sequence analysis of the hepatocyte nuclear factor 1? gene showed that Iraqis have mutational hot spot at exons 3 and 4 of this gene responsible for maturity onset diabetes of the young type 3, and that mutations in the promoter region or exons 5, 6, 8 and 9 are a rare cause of diabetes.

دراسات وراثية خلوية ومظهرية لثلاثة انواع من شبوطيات المياه العذبة العراقية (Barbus luteus, Cyprinion macrostomus, Chondrostoma regius) Three Species of Iraqi Freshwater Cyprinidaes (Barbus Luteus, Cyprinion Macrostomus, Chondrostoma Regius)

اسم المؤلف: اسماء سامي ابراهيم
اسم المشرف: ندى عبد المجيد الانصاري طه ياسين الدوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان - الوراثة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية التعرف على الطراز الكروموسومي ونظام تحديد الجنس والتمييز بين الجنسين مظهريا في ثلاثة انواع من اسماك المياه العذبة العراقية ممثلة بسمكة الحمري Barbus luteus والبنيني كبير الفم macrostomus Cyprinionوالبلعوط الملوكي Chondrostoma regius | These present studies were carried out to determine the karyotypes & the system of sex determination between the two sexes as well as the sexual morphological characteristics of three species of Iraqi fresh water fish Barbus luteus, Cyprinion macrostomus, & Chondrostoma regius that were fished from Tigris river at Baghdad during 2005. The cytogenetic studies revealed that the chromosome number of Barbus luteus is 2n = 148 & the karyotype of males is (80m + 52sm + 16st) while the females has (80m + 51sm + 17st). The chromosome number of Cyprinion macrostomus is 2n = 50 the males has (6m + 24sm + 12st + 8t) while the females has (6m + 23sm + 13st + 8t). The chromosome number of Chondrostoma regius is 2n = 48 and the karyotype is (14m + 30sm + 4st). Sex chromosomes were identified in Barbus luteus & in Cypinion macrostomus for the first time , the sex is determined by ZZ / ZW system, the female was considered as heterogametic (ZW) while the male was considered as homogametic (ZZ) , & the chromosome Z was represented as submetacentric chromosome & chromosome W as small subtelocentric chromosome. In spite of the difference in their chromosome number , the ratio between red blood cell diameter & it's nucleus was approximated in these species. The differences among these species in both of the diameter of cell and the nucleus didn't match with the differences at the polyploidy levels or with the difference in chromosome number within the same level. The liver & intestine were recommended as new sources for chromosomal studies , because of the good ratio of dividing cells (71.4 % & 58.2 %) respectively from the whole dividing cells in kidney that was considered as a control. The crude aqueous extract of fresh convolvulus arvensis leaves showed good effect in arresting the cell division of fish at metaphase , the concentration (0.4, 0.6, 0.8, ) mg / gm arrested kidney cells of carp fish at the metaphase in ratios 71.1 % , 88.1 % , 94 % respectively from the colchicines treated kidney cells (control). Positive significance correlation was found (r = 0.9518) between the percentages of arrested cells at metaphase & the concentrations of crude extract. Comparison between the females and males of the three species revealed that in Barbus luteus the distance from head edge to the anal orifice & from head edge to the dorsal fin were larger in males than females. The females have dark colored fins with reddish ventral aspect of head & body in comparison with the males during the whole months of study. So , they were considered as permanently sexual dimorphism characteristics. But , during the spawning seasons (May & June ) a more brighting color of male with reddish coloration of both sides of body were noticed in comparison with female with a golden orange corneal coloration in both sexes. Thus, they were considered as temporary sexual dimorphism characteristics. In Cyprinion macrostomus the distance from the head edge to the anal orifice was larger in females than males. The colors of anal & caudal fins were darker in females than in males during the whole monthes of study.So , they were considered as perminantly sexual dimorphism characteristics. While, during the spawning seasons ( May , June , July & August) , the gill operculum was of orange color only in male that extend to the end of pectoral fin. So , this was considered as a temporary sexual dimorphism characteristic. In Chondrostoma regius the distance from the head edge to the dorsal fin & from the dorsal to caudal fin as well as the pectoral fin base length were larger in females than in males during the whole months of study it was considered that they are permanently sexual dimorphism characteristics. Differential characteristics were not noticed during the spawning season (February & March). The permanently sexual dimorphism & the general characteristics were not affected by the differences of standard length , weight , age & season , because they were observed in fishes of different lengths , weights & ages & during the months of study 2005. But , the seasons affect the development of temporary characteristics in Barbus luteus & Cyprinion macrostomus , because these characteristics were observed during the spawning season only. Aging of Barbus luteus was accompanied by growth increase as indicated by the mean of standard length during May , July, August, September & December & by the mean of weight during May , August, September, October & December. A positive correlation was found between standard length & weight during the months of study , with the exception of January. While aging of Cyprinion macrostomus & Chondrostoma regius was not accompanied by growth increase as indicated by the means of standard length & weight , but a positive correlation between standard length & weight was found during the studied months. Same growth type were observed in the three species of fish , it was allometric in two sexes.

دراسة تصنيفية مظهرية لبعض انواع عائلة قفازات الضفادع من رتبة متشابهة الاجنحة Homoptera : Cercopidae من بعض محافظات العراق Taxonomic Morphological Study of Some Species of Froghoppers, (Homoptera : Cercopidae) From Some Iraqi Governorates

اسم المؤلف: حلا تايه عمران
اسم المشرف: حسن سعيد جاسم الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمن البحث دراسة تصنيفية مظهرية لخمسة عشر نوعا تابعة لاربعة اجناس لعويلتيCercopinae، Aphrophorinae لعائلةCercopidaeمن رتبة متشابهة الاجنحة Homoptera جمعت من مناطق مختلفة في العراق تم الاعتماد على النوعCercopis vulnerata(Rossi, 1807) نموذجا للعائلة.وهذه ا | The present study comprisers taxonomic morphological of 15 species belong to four genera of the two subfamilies Cercopinae and Aphrophorinae, belong to the family Cercopidae of the order Homoptera, it has been regarded the speciesCercopis vulnerata(Rossi, 1807) as typical sample of the families.The species studied in were : Family : Cercopidae West wood, 1838Sub family : Cercopinae West wood, 1838.Genus : Cercopis Fabricius, 1775.**Cercopis vulnerata (Rossi, 1807)Sub family : Aphrophorinae Amyot&Serville, 1843.Genus : Aphrophora Germar, 1821.**Aphrophora alni(Falle'n, 1805).**Aphrophora major (Uhler, 1896).**Aphrophora oblique(Uhler, 1896).**Aphrophora salicina(Goeze, 1778)**Aphrophora straminae(Kato, 1932).**Aphrophora forneri(Haupt, 1919).**Aphrophora intermedia (Uhler, 1896).*Aphrophora triticasp.nov.*Aphrophora asadisp.nov.Genus : Phileanus Stal, 1864.Phileanus spumarius (Linnaeus) 1758.Genus : Neophileanus Haupt, 1935.** Neophileanus lineatus(Linnaeus, 1758).**Neophileanus campestris(Falle'n, 1805).**Neophileanus longiceps(Puton, 1895).**Neophileanus exclamationis(Thunberg, 1874). *New species for the science.**New recorded to the Iraq insect fauna of Homoptera.

التحري عن التشوهات الكروموسومية وطفرات المورث FLT3/ITD في مرضى ابيضاض الدم النخاعي الحاد Screening For Chromosomal Aberrations And Gene Mutations FLT3/ITD In Patients With Acute Myeloid Leukemia

اسم المؤلف: سمارة كاظم محمد
اسم المشرف: عبد الحسين مويت الفيصل
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: صممت الدراسة الحالية من اجل تسليط الضوء على تاثيرات مرض ابيضاض الدم ((AMLفي بعض معايير الوراثة الخلوية والجزيئية الحاصلة في كريات الدم البيضاء في ثلاثة حالات للمرضى اشتملت على مرحلة ما قبل العلاج, في اثناء العلاج وبعد العلاج الكيميائي. كما شملت الدراسة ا | The present study was designed to shed light on the cytogenetic and molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three stages before, during and after treatment with chemotherapy (in vitro) in lymphocytes. It was also aimed to investigate chromosomal aberrations, micronucleus formation and FLT3/ITD point mutations in CN - AML region 100 - 300 bp compared with healthy control. The study was comprised of forty - seven AML Iraqi patients their ages ranged between 2.5 - 81 years. It included twenty females and twenty seven males compared with twenty - six healthy control. The following results were obtained : AML is most common leukemia in adults and uncommon in children. It was found that 46.8% patients suffer from AML, who were under 15 years old, while 19.15% patients their age ranged between 16 - 30 years; 19.15% of patients their age were more than 45 years and 14.9% of patients their age ranged between 31 - 45 years of the total AML patients. AML is more common in males than females. The percentages of females and males were 42.5% and 57.5% respectively representing 1.35 : 1.00 male : female ratio. Two AML patients 5.3% have diploid cells when examined under light microscope. The highest mitotic index was 7.498±1.7 occurred in patients before taking treatment while recorded 6.784±4.5 during treatment and 7.000±2.5 after treatment. Nuclear anomalies such as nucleoplasmic bridges and nuclear buds were observed in AML patients, Micronucleus mean values recorded 0.033±0.018, 0.020±0.00015 and 0.036±0.01for AML patients before, during and after treatment respectively when compared with the control which recorded 0.002±0.00002. Nuclear division index (NDI) means before, during and after treatment of AML patients were 1.658±0.2, 1.000±0.022 and 1.424±0.19 respectively. Significant differences occurred among the three groups when compared with the control group (1.282±0.09). Extracted DNA from 30 AML patients was amplified by PCR to obtain FLT3/ITD gene from exon 11 to intron 12 and ten of them sent for sequencing. Two patients showed larger bands (470 and 460) bp when compared with wild type (330) bp. Among six patients, three displayed point mutations of deletion and substitution while others were normal since no mutations were detected. The percentages of mutation types were substitution 57.78% and deletion 22.2%. The effect of mutations was missense mutation (55.54%), deletion (22.22%) and nonsense (22.22%). It has been concluded from the current work that AML is more common in adult males, patients suffer from AML exhibited high MI, NDI, MN and point mutations are present in such patients including deletion and substitution causing missense, nonsense and deletion.

تقييم الواقع البيئي لمعمل بابل /2 للبطاريات Assessment Study For The Environmental Situation of Babylon\2 Batteries Plant

اسم المؤلف: منال مالك سعدون الموسوي
اسم المشرف: سهير ازهر الكاظمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In this study was investigated the quantity and quality of industrial water from (Babil/2 batteries plant in Baghdad) and estimate the amount of air pollutants for some factory sections and estimate the amount of solid waste resulting from the industry and study the impact of these pollutants on the environment and public health.The first target of this study is investigated the physical and chemical characteristics of the discharged water from sections shipping and ficus where the sample water collocated was two samples per month for 6 month was taking during period from Novembers 2013 to May 2014. Some measurements were conducted laboratory and others on site, the result showing the following values of the variables rates for the shipping and ficus sections before treatment and respectivelyResults of the study showed that there is a significant increase inThe concentrations in sulfate and TDS, heavy metal When reached after treatment for the same variables and respectivelypH 9.9, EC 1524.9 ?s/cm, TSS 166.4mg/l, TDS 611.7mg/l, Cl - 64.46mg/l, (SO4)+2 486.8mg/l, BOD 49.2mg/l, COD 220.4mg/l, Pb+2 0.55mg/l, Zn+2 0.09mg/l, Cd+2 0.01mg/lpH 8.2, EC 1191.6?s/cm, TSS 68.1mg/l, TDS 927.6mg/l, Cl - 76.95mg/l (SO4)+2, 722.6mg/l, BOD 4.4mg/l, COD 29.2mg/l, Pb+2 0.04mg/l, Zn+2 0.03mg/l, Cd+2 0.01mg/lFrom the results of the study showed that there is a no significant increase in the concentrations in sulfate and TDS, but increases slowly. In the water of charging department reached the concentration of same variables and respectivelyThe second aims of this study a steamed the concentration and rate of air pollution (Total Suspended Particles, heavy metal, Oxides and gases) collected the sample of outstanding minutes analysis made to know and identify the concentration of heavy metal for same factory section and compared those with local and global determinants also the concentration of air emitted was measured.Results of the study showed that there is a significant increase in the concentrations of the outstanding minutes compared to the allowable limits as it reached the highest concentration in the Department of Shipping 3313.46?g/m3. The lowest concentration was in the section of the Aggregation 0.8155?g/m3. Where the concentration of heavy metals, especially lead element exceeded the permissible limits as it reached the highest concentration in Ficus section 3.53?g/m3 the lowest concentration in the assembly department which is 0.36 ?g/m3 concentration of CO gas in the department of Ficus, Shipping, assembly, queries reachedpH 1.6, EC 12058?s/cm, TSS 148.3mg/l, TDS 10122mg/l, Cl - 76mg/l(SO4) +2 3838mg/l, BOD 23.55mg/l, COD 203.5mg/l, Pb+20.55mg/l Zn+20.46mg/l, Cd+2 0.022mg/lrespectively.(21.7, 3.9, 2.44, 2.84?g/m3), the concentration of CO2 was 231.8, 187.6, 219.4, 225.4?g/m3.In the department of Ficus, Shipping, assembly, respectively queries either NO2 was 0.12?g/m3 in sections of Ficus, Shipping and queries, 0.16?g/m3 in the assembly department. The H2S appeared only in sections Ficus, Shipping with concentration 0.02?g/m3, 0.18?g/m3 respectively, either gas SO2 only appeared in the shipping department and was only 0.01 ?g/m3.The last part of these study was to calculate the amount of solid waste for a period of study in terms of total solid waste generated by the plant was 37.004 tons for eight months and included this waste pastes lead and lead plates and scrap processing units either the amount of recycling of batteries that are broken in the foundry of lead in Khan Dhari amounted to 354.832 tons for eight months, and the output of 181.1 tons. In this study we foxed on the problems in these factory that effected the process of industry and we found liquid wastes in the factory is not efficient in reducing the concentrations of all the variables treatment and gaseous emissions generated in the factory, there are no have any significant treatment of solid waste recycled and are utilized as it emerged that the Babil / 2 battery plant on a large degree of pollution sulfate and TDS with regard to liquid waste. The minutes and the outstanding lead with respect to gaseous pollutants to the current study that recommended a set of recommendations that would work to eliminate or minimize the causes of pollution and thus access to good results.

الكشف عن بعض عوامل البيئة الداخلية للخلايا السرطانية للنساء العراقيات المصابات بسرطان الثدي Detection of Some Microenvironment Factors In Tissue Samples of Iraqi Women With Breast Cancer

اسم المؤلف: فاطمة سمير عبد الرزاق الزبيدي
اسم المشرف: اخلاص مشرف عيدان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Breast cancer (BC) is the most common cause of cancer - related deaths in women. It continues to rank as one of the top killer of women. This cancer increased in frequency in the last years in Iraq. The aim of this study was to shed light on the immunohistochemical for some factors that could be affect on development of microenvironment in breast cancer of Iraqi patients. And these factors include CD133 as a marker for breast cancer stem cells (BCSs), and also studying tumor necrosis factor alpha (TNF - ?) and transforming growth factor beta (TGF - ?). 53 samples Formalin - fixed, paraffin - embedded (FFPE) wax block appeared their ages were range from 29 - 70 year with a mean age of 48.45 years. 32 patients with breast carcinoma and 21 patients with benign breast lesions were included in this study for comparison. The results of this study show that the CD133 positive expression was found in (56.2%) of Iraqi breast cancer cases. Also the result of this study show that (62.5%) positive expression from both (TNF - ? &TGF - ?) of breast cancer cases, compared with sample of benign breast lesion. The results show (52.3%) positive expression of TNF - ? and (28.57%) positive expression of TGF - ? of samples with benign breast lesion, there is a significant different between studied samples, compared with (19.05%) samples positive expression of CD133 of sample with benign breast lesion so there is a significant different between the samples with breast cancer and benign breast lesion. Also the results show there is a positive relationship correlation between (TGF - ?) expression and (TNF - ?) expression, while there were no relationship correlation between (TGF - ?) and CD133 and no relationship correlation between TNF - ? and CD133. The results show there is a positive correlation between the grade and breast cancer with the three different expressions of marker but in different strongest correlations between (TNF - ?) and (TGF - ?) with the graded but this correlation becomes weak with CD133 marker the value of significant. According to the relationship in breast cancer case in this study between the studied markers and stage of case it shows (TNF - ?) has a strong positive correlation while the correlation appear week between the stage of this studies case and end each of TGF - ? and CD133. For this we concluded from the results there high expression of CD133 and TNF - ? indicators and TGF - ?. CD133 could use in diagnosis of the cancer cell and the high expression of TNF - ? & TGF - ? indicate that these factor play important roles in tumor microenvironment metastasis. And the strong correlation between the expression of these markers with grade and stage of breast cancer

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

اسم المؤلف: كرم رياض حسن الجراح
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).

التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients

اسم المؤلف: احمد كاظم محمد
اسم المشرف: محمد ابراهيم نادر بتول حسن الغرابي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: داء السكري مرض واسع الانتشارعالميا تختلف نسبة الاصابة بين البلدان المتطورة والنامية ويعتبر المسبب الرئيسي للاعاقة والموت في العالم.داء السكري النوع الثاني هو الاكثر حدوثا حيث تبلغ نسبة الاصابة (90 - 95%) من مجموع المصابين بالسكري للانواع الثلاثة الرئيسية. | Sixty of non insulin dependent diabetes mellitus (NIDDM) patients who were diagnosed according to American Diabetes Association criteria (ADA) 2007 were selected from the specialized center of endocrinology and diabetes (Baghdad AL - Russafa Health Directorate) during the period between first of May 2013 to last of October 2013.Their age ranged 35 - 70 years. Fourty apparently healthy individuals their age ranged (35 - 70) years were considered as control. Enzymatic colorimetric methods used for measured FBS (fasting blood sugar) and HbA1c (glycohemoglobin) and enzyme linked immunosorbent assay (ELISA) for hormones and enzyme markers. Fasting blood sugar revealed high significant in patients with median (11.6 mmol/L vs. 5.9 mmol/L) and (P<0.001) in comparison to control groups.Elevation of glycated hemoglobin (HbA1c) with mean (9% vs. 5.5%) in comparison to control groups. Another reliable marker are the concentration Adpionectine hormone, Insulin hormone and adenosine deaminase activity the results of those estimated significantly difference between levels mean (20.7 vs. 34 ng /ml) in patients compared to healthy subjects (P<0.001) for adiponectin ; mean (106.6 vs. 59.3 ng/ml) for ADA (adenosine deaminase) with (P<0.001) and the median (12.1 vs. 16 uIU/ml) for insulin hormone with (P 0.001). In order to investigate the accuracy of previously mentioned tests, a statistical analysis [Receiver - Operator Characteristics (ROC)] has been applied to show the accuracy, specificity and sensitivity of the tests under test.This analysis revealed that serum ADA activity is the best marker with highly specificity 100%, sensitivity 100%, and accuracy 100% while; FBS was the best test with highly specificity 100%, sensitivity 100% and 100% accuracy in comparison with other tests. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction - Sequences Specific Olegionucleotide (PCR - SSO) technique. The present study revealed that diabetic patients were positively associated with HLA - DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison with healthy control groups.This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2(Homeostatic Model Assessment2) ? - function, HOMA2 - IR, serum adiponectin, serum ADA and HLA alleles(DQB1*0201, DRB1*1137, DRB1*0401, DQB1*0501, DRB1*1306) in spite the significant associated between FBS and serum ADA and HLA - DRB1*0701 allele with (P 0.021, P 0.008) respectively.The current result concluded that there may be an important role for HLA genotyping in arising the chance for enhancing the susceptibility for either disease development or protection against its initiation.

انشاء وتوصيف لخط سرطان الثدي الخلوي العراقي Establishment And Characterization of Iraqi Breast Cancer Cell Line

اسم المؤلف: مرتضى عادل الشامي
اسم المشرف: محفوظة عباس عمران احمد مجيد الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Several primary cultures were initiated from different samples obtained from Iraqi female patients of breast tumor, one sample was successful, and this sample was histological diagnosed as breast cancer infiltrating ductal carcinoma.The cell suspension was cultured in tissue culture flask and confluent monolayer achieved after 16 days from primary culture. The continuous subculture was done in grown cells in tissue culture flask each 48 - 96 hrs. Between subculture to other until across 50 passages through11months.In our current study different experiments were done to characterize the cultured continuous cells, which are studying the growth curve of the new established cell line and calculating the population doubling time that have been 22 hours.Furthermore, a morphological study was carried out by staining the cells with hematoxilin and eosin dyes. The cells were elongated multi - polar epithelial like cells with nuclear polymorphism and multi - nuclei, in addition to high nuclear to cytoplasm ratio, all these characters of the malignant tumor cells.The Cytogenetic study showed chromosomal aberrations with many numerical changes among the tumor cells and abnormal structure gives chromosomes with unknown origin called marker chromosome. In furthermore the G - band stained of normal 46XX chromosome was done to facilities the comparisons between chromosomes of the new established breast cancer cell line and normal chromosomes aberrationsImmunocytochemistry examination was done for the tumor cells grown in multi well tissue culture slide chamber to detect the presence of some hormonal receptors (ER and PR) gives negative result, and to test Her - neu2 gene that gives week positive result.

تاثير فطريات المايكو راي زا الشجيرية والمادة العضوية في مرض الذبول المتسبب عن الفطر Fusarium oxysporum f.sp.lycopersici في نبات الطماطة Lycopersicon esculentum Effect of Arbuscular Mycorrhiza And Organic Matter On Wilting Caused By (Fusarium Oxysporum F.Sp. Lycopersici) In Tomato (Lycopersicon Esculentum)

اسم المؤلف: ثامر عبد الشهيد محسن علي الابراهيمي
اسم المشرف: بتول زينل علي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The study was conducted to evaluate the effect of three arbuscular mycorrhizal species (Glomus etunicatum, G. leptotichum, Rhizophagus intraradices) and the organic matter (Peatmoss) on controlling the wilting caused by Fusarium oxysporum f.sp.lycopersici on tomatoes using pot cultures. The study was included two experiments : 1 - The first experiments was performed to select the best mycorrhizal species or a maxture of two or three species acting on several parameters, Results showed the followings : - Significant increase of the tri mixture activity of the three mycorrhizal species compared to the dual mixture or single species on mycorrhizal colonization, mycorrhizal dependency and the weight of mycorrhizal roots after four and eight weeks planting. - The highest activity of the trimixture of AM fungi in increasing the plant growth parameters after four and eight weeks planting. - The highest activity of the tri mixture of AM fungi in increasing the percentage of N, P, K and the protein in leaves and roots after 8 weeks planting compared to other treatments.2 - The second experiment was performed to study the effect of the tri mixture of AM fungi (which gave the highest activity in the first experiments) and the organic matter in the presence of the pathogen F.o.l. and their interactions on mycorrhizal parameters, disease parameters, analysis of some elements and compounds, study of some plant growth parameters, enzymatic and non - enzymatic antioxidants in tomato plant, Results revealed the followings : - Significant increase in mycorrhizal colonization included % of mycorrhizal frequency, mycorrhizal intensity in the root system, mycorrhizal intensity in in root fragments, abundance of arbuscules in parts of root fragments, abundance of arbuscules in root system and the highest values were shown in the treatment (organic matter x 4weeks pathogen infection) after six and ten weeks planting. - Significant increase in dry weight of mycorrhizal roots, the highest was shown in the treatment (organic matter x control) after 10 weeks planting. - Increase in percentage of mycorrhizal dependency after 6 and 10 weeks planting and the highest percentage was shown in the treatment (absence organic matter x control) after 10 weeks. - Increase in number of mycorrhizal spores, the highest was recorded in the treatment (organic matter x 4weeks pathogen infection) after 12 weeks. - Significant decrease in % of disease parameters in leaves and roots, the lowest percentage were recorded by the treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) for all parameters. - Results of plant growth parameters showed significant increase of all for different weeks. The treatment (trimixture of mycorrhiza x organic matter x control) recorded the highest for all parameters tested in plant. - The treatments affected the flowering and fruit duration, the shortest was shown by the treatment (trimixture of mycorrhiza x organic matter x control) - Significant increase in percentage of N, K and protein in leaves and roots, and chlorophyll a, b and total chlorophyll in leaves, the highest values were shown in treatment (trimixture of mycorrhiza x organic matter x control). - The treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) recorded the highest percentage of P, souble carbohydrates, highest content of malondialdehyde and hydrogen peroxide in leaves and roots as well as highest percentage of lignin in roots. - Significant increase in the activity of antioxidant enzymes in leaves and roots, the highest activity recorded by the treatment (trimixture of mycorrhiza x absence organic matter x 4weeks pathogen infection). - Significant increase of non - antioxidant enzymatic in antioxidants included content of carotene in leaves, content of ascorbic acid, proline, total phenol, and glutathione in leaves and roots, The treatment (trimixture of mycorrhiza x organic matter x control) showed the highest content of carotene and ascorbic acid while the treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) showed the highest content of proline, total phenol and glutathione in leaves and roots.

دراسة تصنيفية لانواع مختارة من نباتات ذوات الفلقتين في محافظة كربلاء المقدسة, العراق Taxanomical Study For Selected Dicot. Spp. In The Holy Karbala Province, Iraq

اسم المؤلف: زينة خليل ابراهيم البهادلي
اسم المشرف: علي حسين عيسى الموسوي خليل ابراهيم الشمري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة بيئية لعدة مواقع من محافظة كربلاء المقدسة بين شهري شباط وايار ولسنتي 2013 و2014 من حيث نوع الترب والمجتمعات الاحيائية لهذه المواقع جمعت خلالها انواع مختلفة من النباتات تابعة لعائلات مختلفة ضمن ذوات الفلقة الواحدة وذوات الفلقتين اذ | The present work includes ecological study for different locations of the holy Karbala province between February and May and for the years2013, 2014, investigating soil kinds and communities in these locations. Different plant species of Monocots and Dicots were collected from variable soil kinds, such as sandy saline soil, loamy saline soil and sandy gravely soil. The species of Haloxylon salicornicum, Zygophyllum coccenium were dominant in the communities of sandy saline soil, Nitraria retusa, Juncus sp. and Halocnemum strobilaceum were dominant in loamy saline soil.Comparative anatomical study has been done for (30) Dicots wild species grown in these environments of Karbala province, as follow : a.Family : Aizooaceae1) Aizoon hispanicum L. b.Family : Chenopodiaceae2) Chenopodium murale L.3) Halocnemum strobilaceum (Pall.)M.Bieb.4) Haloxylon salicornicum (Moq.)Bunge ex. Boiss.5) Salicornia herbacea L.c. Family : Compositae (Asteraceae)6) Carthamus oxycantha M.B. 7) Launaea capitata (Spreng.)Dany 8) Launaea mucronata (Forssk.)Muschl. 9) Rechardia tingitana (L.)Roth. d. Family : Convolvulaceae10) Convolvulus oxypyllus Boiss. e. Family : Cruciferae (Brassicaceae)11) Diplotaxis harra (Forssk.)Bioss. 12) Sterigosella africana (L.)Boch. 13) Sterigosella grandiflora (Bnge)Bochantsev 14) Savignya parviflora (Del.)Webb. 15) Neotorularia torulosa (Desf.) Hedge & J. Leonard 16) Zilla spinosa (Turra)Prantl. f. Family : Cucurbitaceae 17) Citrullus colocynthis (L.)Schrad. g. Family : Geraniaceae18) Erodium laciniatum (Cav.) Willd.h. Family : Malvaceae19) Malva parviflora L. i. Family : Neuradaceae 20) Neurada procumbens L. j. Family : Nitrariaceae21) Nitraria retusa (Forssk.)Aschers.22) Peganum harmala L. k. Family : Papillionaceae23) Trigonella stellata Forssk. m. Family : Polygoniaceae 24) Rumex versicarius L. n. Family : Resedaceae25) Oligomeris linifolia (Vahl)Macbr.q. Family : Solaneaceae 26) Lycium barbarum L.r. Family : Tamaricaceae 27) Tamarix florida Bge.28) Tamarix macrocarpa (Ehrenb.)Bge.u. Family : Zygophyllaceae29) Fagonia glutinosa Del. 30) Zygophyllum coccenium L. The present research focused on many anatomical characters and studies comparatively, such as epidermis types, stomatal complexes, leaf venation, cross sections of root, stem and vertical sections of leaf blades. Different cross sections were shows variability cuticle thickness and epidermis, vascular cylinder tissues, collenchyma and sclerenchyma in different species studies. Variations were noticed among these tissues in the variable species.Thickness of cuticle epidermis of leaves were variable, and unifacial and bifacial mesophylls were noticed for the first time in some species studies. Some cross sections were obtained for scale leaves in Tamarix florida, Tamarix macrocarpa, Halocnemum strobilaceum, Haloxylon salicornicum and Salicornia herbacea.The anatomical characters of these plant parts has not been studied before for these species, at least for Iraqi specimens. The following species Launaea capitata, Oligomeris linifolia and Zilla spinosa were glabrous in all vegetative and reproductive organs. In time that the other species, Haloxylon salicornicum , Salicornia herbacea, Launaea mucronata, Reichardia tingitana, Strigosella grandiflora , Peganum harmala, Lycium barbarum, Tamarix florida and Tamarix macrocarpa were glabrous just in the vegetative parts, but hairy (pubescent) in the reproductive organs. The indumentums was variable in thickness within variable plant organs and parts, even on the lower and upper leaf surfaces. Different kinds of crystals were found and verified in the stems and leaves of different species. Size and density of these crystals were variable in variable locations of stem and leaf tissues.. Geographical distribution was investigated for all the studied species using Iraqi satellite maps by GIS program. Neotorularia torulosa was the most distributed species, and Rumex versicarius was the least distributed one. Schedules, schemes, illustrations and micrographs were presented for the different species studied in this work. Lastly results were discussed depending on available references and reasons.

دراسة تصنيفية لانواع الجنس Echinops L. (Compositae) في العراق Comparative And Systematical Study of The Genus Echinops L. (Compositae) In Iraq

اسم المؤلف: سكينة عباس عليوي
اسم المشرف: علي حسين عيسى الموسوي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تصنيفية مقارنة لانواع الجنس Echinops L. في العراق حيث شملت الصفات المظهرية والتشريحية وحبوب اللقاح، وكذلك البيئة التوزيع الجغرافي فضلا عن المعاملة التصنيفية. شملت الدراسة المظهرية دراسة صفات الجذور والسيقان والاوراق والرؤوس الزهري | The present investigation dealt with comparative systematic study of the genus Echinops L. (Compositae) in Iraq.The work involved comparative morphological and anatomical characters, in addition to palynology, ecology, geographical distribution and taxonomic treatment for these species. Morphological characters of roots, leaves, involucre bracts, inflorescence, in addition to, indumentum variations were discussed and the study concluded that stems, leaves, involucres bracts, especially the inner most layer characters were useful aid in distributing species of the genus. Comparative anatomical characters were used, such as characters of leaves, epidermis stomatal complexes, variation patterns, cross sectionings of stems, leaves as well as L.S of growing points. The study revealed that the anatomical characters are important taxonomically. The present investigation observed that pollen grains of all species were tricolporate and echinate. The taxonomic important of grains characters were assessed of the species. Maps of geographical distribution were prepared for all species studied. The study shown that most species were distributed in the North area of Iraq and a few species of the genus were distributed in the East and South of Iraq. In conclusions, (11) species of the genus Echinops were recognized in Iraq. Taxonomic treatment was carried out for all Echinops species and key was designed for them

دراسة تصنيفية مظهرية لبعض انواع عائلة فراشات اللهانة من رتبة حرشفية الاجنحة (Lepidoptera : Pieridae) من مناطق وسط العراق وشماله Taxonomic Morphological Study of Some Species of Cabbage Butterflies, (Lepidoptera : Pieridae) From Mid And North Regions of Iraq

اسم المؤلف: مهند محمود اغا
اسم المشرف: حسن سعيد جاسم الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study comprises taxonomic morphological of seven species belong to six genera of the subfamilies Pierinae, Coliadinae, (Pieridae : Lepidoptera) which were collected from middle and north of Iraq.The described species in this study are : Famliy : Pieridae Duponchel - Subfamily : Pierinae DuponchelGenus : Pieris Schrank, 1801Pieris rapae Linnaeus, 1758Pieris brassicae Linnaeus, 1758Genus : Belenois Moor, 1881Belenois aurota Fabricius, 1793Genus : Colotis Hübner, 1816Colotis fausta Olivier, 1804Genus : Anthocharis Boisduval, 1836Anthocharis cardamines Linnaeus, 1758 - Subfamily : Coliadinae SwainsonGenus : Colias Fabricius, 1807Colias croceus Fourcroy, 1785Genus : Gonepteryx Leach, 1785Gonepteryx rhamni Linnaeus, 1758The last species as new recorded to fauna of Iraq

التمنيع باضداد الخمل النوع الاول المنتجة من بكتريا الاشريكية القولونية المسببة لالتهاب المجاري البولية ضد استيطان واصابة المثانة Immunization With Type 1 Fimbriae of Uropathogenic Escherichia Coli Against Colonization And Infection of Bladder

اسم المؤلف: اروى علي شكر
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Between September and November 2013 a total of 140 urine specimens obtained from patients in Al - Kadhmiah Teaching Hospital, Ibn - Al Naffees Teaching Hospital, and Educational Laboratories in Medical city. These midstream urine specimens were obtained from patients clinically suspected to have urinary tract infection (UTI) in sterilized containers. All specimens were processed immediately after collection.A total number of 140 specimens of mid - stream urine were collected from patients suffering urinary tract infection symptoms. These isolates were obtained from 41 male and 99 female patients. They were distributed in the age group of 5 - 60 years old. All specimens were identified using biochemical tests and API 20 E system. 60 (42.85%) of urine specimens was Escherichia coli and 15(10.71%) of specimens growth was Klebsiella spp. and 25(17.85%) growth was Proteus spp. and 40 (28.57%) was G+ve bacteria.The adherence ability of E.coli isolates were evaluated by using Congo red agar and detected their ability to produce biofilm by using methylene blue staining technique in polystyrene microtiter plates and then Optical density was determined at 580 nm.All sixty isolates of E.coli were grown on Congo red agar medium to detect their adherence ability. Twenty (33.3%) isolates were given negative result by forming pink colonies on congo red agar, were as, forty (66.6%) isolates were given positive result by forming black colonies with a dry crystalline. Three isolates give strong results E.coli (13, 8, and 40). Consistency indicated biofilm production by microtiter plate. E. coli 40 which isolated produced the thickest biofilm (O.D. : =3.17).Type 1 fimbriae expression by E.coli 40 isolate was detected by mannose - resistant hemagglutination (MRHA) of human blood group (O) IRBCs in the presence of 2% D - mannose. The isolate showed strong MRHA characteristics of type 1 fimbriae under microscope (40x). The E.coli E.40isolate which produced the thickest biofilm and gave strong MRHA was elected to be the source of type 1 fimbriae.Type 1 fimbriae was extracted by heating and mechanical shearing and partially purified by Ultracentrifugation (48, 000xg for 3hrs). SDS - PAGE with a molecular weight 20, 000 Dalton.Anti - type1 fimbriae antisera were prepared in rabbits. The specificity and titration of anti - type1fimbriae antibodies were determined using IgG protein by radial immunodiffusion plate and bacterial agglutination. IgG antibodies to fimbriae type 1 were also detected in rabbit sera from immunized rabbit and non - immunized. Immunized rabbit test 1 had increases in Fim1 - specific IgG antibodies (9.5) mg/dl. Immunized rabbit test 2 had increases in Fim1 - specific IgG antibodies (8.6) mg/dl. The non - immunized rabbit had no increase in absolute IgG antibodies to Fim1.The antisera agglutinated bacterial cells up to 1/80 dilution confirming the presence and titer of specific antibodies against type1 fimbriae.The microtiter plate method was applied to estimate the role of the prepared anti - type1fimbriae antibodies in reducing the biofilim formation by E.coli. Prevention of bacterial adherence and subsequent biofilm formation to polystyrene microtiter plate was studied by employing different dilutions (1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640) of rabbit sera containing anti - type 1 fimbriae antibodies. The maximum inhibition of biofilm formation in terms of optical density (580 nm) was found in lowest dilution (1/10) (highest concentration of Abs). However, the minimum inhibition was observed in highest dilution of rabbit antisera (1/640) (lowest concentration of Abs). Here the inhibition occurs in a dose dependent manner as the biofilm formation increased dramatically with increasing in antisera dilution. The results also showed that there is a significant difference(P<0.05) among data treated with sera and data without sera and among serial dilutions.General urine examination and culture for urine that absorbed from test 2 and control was done (GUE) show that pus cell in urine was (1 - 2/HPF). There was no growth of bacteria in urine culture.The Histological section show that the Control and test 1 rabbit (have been immunized with fimbriae and adjuvant and injected with E.coli intra muscular) and Test 2rabbit (that has been immunized with fimbriae and adjuvant and injected with E.coli directly in bladder has normal epithelial cells and mucosa.

التقييم الجزيئي لنسخ جين المقاومة الدوائية MDR1 في بعض المرضى العراقيين البالغين المصابين بسرطان ابيضاض الدم الحاد Molecullar Assessment of Multidrug Resistance Gene (MDR1) Transcript In Some Adult Iraqi Patients With Acute Leukemia

اسم المؤلف: كفاح جبار شاكر اليعقوبي
اسم المشرف: عبد الحسين الفيصل
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aims to shed light on the follow up of acute leukemic (AL) patients at initial diagnosis and after treatment to assess the response and early relapse through evaluating the gene expression level of one of the major multidrug resistance genes which is the multidrug resistance 1 (MDR1) to investigate the possible association between level of MDR1 gene expression and the clinical outcomes and this may be considered as a potential marker for response to chemotherapy of acute leukemic patients. Furthermore, the current study correlates between the MDR1 gene phenotype and MDR1 genotype in three important coding regions (C1236T, G2677T/A, and C3435T considering the potential influence of altering MDR1 activity and its effect on therapy outcome as well as susceptibility to develop cancer.White blood cells (WBCs) isolated from 106 blood sample of acute leukemic patients were provided by Iraqi hospitals in Medical City. These samples were distributed as follows : 46 newly diagnosed patients with acute leukemia who had not received chemotherapy and follow - up 25 after 1st induction, 17 after 2nd induction and 8 at consolidation, with 10 blood samples of healthy voluntaries. Two comparative groups were taken included 46 sample of peripheral blood (PB) and 26 sample of bone marrow biopsy (BMB) in paraffin blocks to validate the level of gene expression compare to WBCs. For genotyping analysis, 56 of blood sample were taken to study genetic variation of MDR1 gene polymorphism. The samples preservation with TRIzol was done. Samples subjected to total RNA and DNA extraction, then molecular study by using reverse transcription, Quantitative Real Time - polymerase chain reaction (qRT - PCR) and direct sequencing, at Molecular Oncology Unit in Guy´s Hospital - Kings College / London.The study reached at the following results : 1 - The results showed age groups (20 - 39 years) were associated with acute myeloid leukemia (AML), while (13 - 19 years) with acute lymphoblastic leukemia (ALL).2 - The level of MDR1 gene expression showed high significant differences with WBCs compared to PB and BMB.3 - The clinical outcomes indicated that the rate of complete response (CR) of newly diagnosed acute leukemic patients was 19(41%), while 27(58.7%) was non - responder (NR).4 - Statistical analysis showed significant differences with NR at initial diagnosis in acute myeloid leukemia, while appeared after 1st induction in lymphoid type.5 - The results of positivity MDR1 gene expression were 10(21.7%) out of 46 newly diagnosed in acute leukemia, while 36(78.3%) were MDR1 - negative depend on (1.1±0.03) cutoff value.6 - The positivity MDR1 gene expression appeared mainly in non - responders patients at initial diagnosis, and with early relapse patients, after complete remission, in consolidation.7 - The MDR1 mRNA expression showed significant differences with high level in NR compared to CR patients at initial diagnosis. During treatment follow up the increased level of MDR1 gene expression in CR patients and appeared non - significantly with NR.8 - The results of MDR1 C1236T genotype and allele frequency showed that 1236CC wild type genotype and C allele were significantly frequent in healthy control. While CT heterozygous genotype frequency was highly significant in AML and no significant difference in allele frequency. ALL showed non - significant difference in genotype and allele frequency of MDR1 C1236T.9 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed no evidence associated with risk factor in MDR1 C1236T ALL carriers. While risk factor observed in AML with MDR1 1236CT carriers.10 - The results of MDR1 phenotype - genotype association indicate that MDR1 1236CC wild type was significantly high expression among healthy and it was aprotective genotype. While the MDR1 1236CT showed significant differences with high level of MDR1 gene expression in AML patients. Whereas ALL revealed significant differences in high level of MDR1 gene expression with MDR1 1236TT genotype. Both CT and TT were affected genotypes.11 - The results of MDR1 G2677T genotype and allele frequency indicated that 2677GA genotype significantly appeared with low frequency in healthy control with no significant difference in allele frequency. Both ALL and AML showed high significant frequency in 2677GT genotype. G allele frequency was showed significant differences in AML while non - significant in with ALL.12 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed the MDR1 2677GT genotype was associated with risk factor to developing ALL and AML. Whereas the GG appeared associated with AML only.13 - MDR1 phenotype - genotype association, indicate that MDR1 2677GA genotype was significantly high expression in healthy individual. While AML patients showed significant differences with high level of MDR1gene expression in 2677GT genotype. ALL showed significant differences with high level of MDR1 gene expression in MDR1 2677TT genotype.14 - The results of MDR1 C3435T genotype and allele frequency showed significant difference in genotype and allele frequency with heterozygous CT in both control and AML patients and mutant T allele. Whereas non - significant genotype and allele frequency with ALL.15 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed that the MDR1 3435CC genotype carriers associated with risk to developing ALL. While no risk factor associate with MDR1 C3435T variants to develop AML.16 - MDR1 phenotype - genotype association, indicate that the wild type 3435CC genotype was significantly high expression in healthy control. The MDR1 3453CT genotype showed high significance with high level of MDR1 gene expression inAML. While ALL showed significantly high level of MDR1 gene expression in 3435TT genotype.17 - The results of MDR1 genotype - phenotype association showed similar impact of MDR1C1236T, G2677T/A and C3435T genotypes in AML clinical outcomes. The MDR1 CT/GT/TT genotypes were associated in NR AML with high level of expression at presentation, compared to significant low level in CC/GG genotype. In contrast, CR patients were observed non - significant with MDR1 gene expression at presentation and significant with low MDR1CC/GG genotypes in post treatment. In regards to ALL patients the MDR1 TT genotype showed significant differences with high level of MDR1 gene expression in NR and CR ALL at presentation and significant only with NR at post treatment. So there was no clear evidence between MDR1 genotypes and clinical outcome with ALL.18 - The haplotype results showed that the three MDR1 C1236T, G2677T/A and C3435T genotype were linkage disequilibrium significantly with heterozygous haplotype B (CT - GT - CT) compared to A(CGC) and C(TTT). Also B haplotype appeared significantly with high level of MDR1 gene expression compared to A and C. According to the clinical outcome, haplotype B was observed significant differences in NR AML patients while other haplotypes were non - significant

اليات استحثاث المقاومة في نبات الخيار بواسطة Trichoderma harzianum وBacillus subtilis وGlomus mosseae اتجاه الفطر Rhizoctonia solani المسبب لمرض سقوط البادرات Resistance Mechanisms In Cucumber Plant By Trichoderma Harzianum, Bacillus Subtilis And Glomus Mosseae Against Rhizoctonia Solani Causing Seedling Disease

اسم المؤلف: رباب مجيد عبد الذهيبي
اسم المشرف: علي هاشم الموسوي هادي مهدي عبود
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت هذه الدراسة للتحري عن اليات استحثاث المقاومة في نبات الخيار ضد الفطر الممرض Rhizoctonia solani بوساطة المعاملة بالعوامل الاحيائية وهي harzianum Trichodermaو Bacillus subtilis وGlomus mosseae وتاثير ذلك في نمو نبات الخيار وحاصله النامي تحت ظروف البيت | This study was carried out to investigate the mechanisms of induced resistance in cucumber plant against pathogenic fungus Rhizoctonia solani by bioeagent factors Tricoderma harzianum fungus, Basillus sibtilis bacteria and mycorrhizal fungus Glomus mosseae. The effect in parameters growth and yield of cucumber plant which growing under greenhouse and field conditions. The pathogenic fungus showed high Pathogenesis on cucumber plant. Three isolated of B. subtilis and T. harzianum showed high antagonism ability against R. solani under laboratory condition. For mycorrhizal fungus G. mosseae isolated was tested to study their ability in cucumber growth for chosen the best isolated which can used in anther experiment which results were as follows : - A - Pots experiment 1 - The bioeagent factors was showed their ability to induced several mechanisms of resistance in cucumber plant against pathogenic fungus R. solani, Which included increasing the activity of defense enzymes in existed of pathogenic fungus R. solani, The treatment (T. harzianum + G. mosseae) was recorded highest level in enzyme activity of Phenylalanine ammonia layase enzyme which was (25.3541) unit/gm fresh weight, While T. harzianum treatment showed significantly increased the enzyme activity of Polyphenol oxidase and Peroxidase which was (10.6707, 2.6137) unit/gm fresh weight respectively. This study showed no enzyme activity for chitinase enzyme excepted in some treatment and some periods.This study recorded increased in plant content of total phenol, lignin, salicylic acid hormone, total protein, The (G. mosseae + B. subtilis) treatment overtaken on other treatments in increased plant content of total phenol which was (1.989) mg/gm fresh weight, B. subtilis treatment was significantly increased plant content of lignin which was (18.318) mg/gm fresh weight, The treatment (T.harzianum+ B. subtilis) showed significantly increased plant content of salicylic acid hormone which was (1.8658) mg/gm fresh weight. This study recorded no Jasmonic acid hormone activity in treated plant except in some treatments but with low level, The treatment (G. mosseae + T.harzianum + B. subtilis) was recorded highest content in total protein which was (239.3) ?g/mg. 2 - The rate of seedling death and disease index was significantly reduced in bioeagent treatments, The lowest rate for seedling death in the treatment of (G. mosseae + B. subtilis), (G. mosseae + T.harzianum) and (G. mosseae + T.harzianum + B. subtilis) which were 11 % each for them comparison with control treatmentwith pathogen which was 100%.3 - The rate number of spores and percentage of mycorrhiza infection was significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. The treatment of mycorrhiza infection with pathogen recorded highest number of mycorrhizal spores which was (1410) spore. The mycorrhiza infection reach to 100% in (G. mosseae + T.harzianum) and(G. mosseae + T.harzianum + B. subtilis) treatment with pathogen fungus. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest index of mycorrhiza infection which reach to (80)%, But the index of mycorrhiza infection and mycorrhizal root weight were decreased in the existed of pathogenic fungus, Mycorrhizal dependency it was increased in all treatments with pathogenic fungus and the treatment of (G. mosseae + B. subtilis) with pathogen recorded highest percentage in Mycorrhizal dependency which was (58.873)%. 4 - The plant content of chlorophyll (a, b, total) was significantly increased, The tratment of (G. mosseae + B. subtilis) with pathogen recorded highest level in chlorophyll a it was (5.30)mg/gm, the highest level in chlorophyll b found in(G. mosseae + T.harzianum + B. subtilis) without pathogen it was (6.07)mg/gm, (G. mosseae + T.harzianum + B. subtilis) treatment without pathogen recorded highest level in total chlorophyll it was (10.79)mg/gm. The concentration of phosphor concentration increased in shoot part of (G. mosseae + T.harzianum) without pathogen which was (0.538)%, potassium concentration were decreased in all treatments with pathogen excepted (G. mosseae + T.harzianum) treatment, The highest level of potassium in treatment of (B. subtilis) without pathogen which recorded (4.65)%, For nitrogen concentration was increased in all treatments with pathogen and the highest level in (T.harzianum) which was (5.114)%. Carbon concentration significantly decreased in all treatments with pathogen and (G. mosseae + B. subtilis) without pathogen recorded highest concentration in carbon which was (46.203)%. 5 - The result showed that all growth parameters either in the absence or presence of pathogenic fungus was significantly increased in all bioagent treatments.The percentage of germination reach to (100)% in (B. subtilis) with or without pathogen , the treatment (G. mosseae + B. subtilis) with pathogen recorded highest level in shoot length it was (92.40)cm, And the same treatment recorded highest rate in fresh and dry weight for shoot part which was (7.412, 0.990) gm respectively, (T.harzianum) without pathogen recorded highest rate in leaf area it was (453.083)cm2, The highest rate of flower number was in (T.harzianum) with pathogen which was (15.66)flower/plant. The fresh and dry weight for root, root length, root surface area and the rate of root diameter were significantly increased in comparison with control treatment without pathogen. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest level in fresh and dry weight for root which were (0.359, 0.094)gm respectively. Highest root length in (G. mosseae + B. subtilis) without pathogen it was (48.14)cm, root surface area decreased in the treatments with pathogen and the treatment (G. mosseae + T.harzianum + B. subtilis) without pathogen recorded highest root surface area it was (19.16)cm 2, The root diameter was increased in(G. mosseae) with pathogen was reach to (0.363)mm.B - Field experiment 1 - Under field condition the percentage of germination increased in the treatment without pathogen which were (94.44)% in treatments (G. mosseae) , (B. subtilis) and (G. mosseae + T.harzianum) respectively, In the treatments with pathogen the percentage of germination reduced and The bioeagent factors showed no reduction in percentage of disease infection or disease index with pathogenic fungi R. solani under field condition which reach to (100)%. 2 - The rate number of spores, percentage of mycorrhiza infection, index of mycorrhiza infection, mycorrhizal root weight and Mycorrhizal dependency on cucumber plant significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. (G. mosseae + T.harzianum + B. subtilis) significantly overtaken on other treatments in increased rate of mycorrhizal spors it was (3950)spore, The mycorrhiza infection reach to (100)% in all treatments, For index of mycorrhiza infection the treatments of (G. mosseae + B. subtilis) and (G. mosseae + T.harzianum) recorded highest rate which reach to (100)%, (G. mosseae + T.harzianum + B. subtilis) treatment recorded high increased in mycorrhizal root weight and Mycorrhizal dependency which were (545.67 gm, 67.78%) respectively. 3 - The plant content of total chlorophyll was significantly increased, The highest level in(G. mosseae + T.harzianum + B. subtilis) treatment which was 5.19 mg/gm. There was significantly increased in the concentration of nutrition elements P, K, N, C in cucumber plant. The treatment (T.harzianum+ B. subtilis) recorded highest concentration in phosphor and nitrogen which were (489.4, 3.62) % respectively, The treatment of (G. mosseae + T.harzianum + B. subtilis) recorded highest concentration of potassium which was (2.975)%. The highest concentration of carbon recorded in B. subtilis treatment which was (34.823) %. 4 - All shoot, root and yield parameters of cucumber were significantly increased under field conditions. The treatment (T.harzianum+ B. subtilis) recorded significantly increased in plant height, dry shoot weight, leaf area rate number of flowers, rate number of fruit and fruit weight which were (42.87 cm, 10.85 gm, 631.06 cm2, 19, 17, 1275 gm) respectively. The treatment G. mosseae recorded significantly increased in fresh shoot weight which was (32.76) gm. The treatment with (T.harzianum+ B. subtilis) recorded significantly increased in fresh and dry weight of root, root length, root surface area, rate of root diameter, rate number of fruit and fruit weight which were (11.4 gm, 5.5 gm, 151.71 cm, 44.05 cm2, 0.763 mm, 17, 1275 gm) respectively,

دراسة مقارنة لتاثير بعض المستخلصات النباتية وبعض الاحماض الكاربوكسيلية في البكتريا المسببة لالتهابات الحروق Comparative Study of The Effect of Some Plants Extract And Carboxylic Acids On Contaminating Bacteria In Burns Infection

اسم المؤلف: سـعاد خليـل ابراهيـم
اسم المشرف: مهدي ضمد القيسي جنان مجيد العقيدي
الموضوع العام: علوم الحياة
السنة: 2009
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت (500) عينة من المرضى المصابين بالحروق من ثلاث مستشفيات في مدينة بغداد للمدة من 1/6/2007 ولغاية 1/11/2007. زرعت هذه المسحات على الاوساط الزرعية للعزل الاولى وتم تشخيصها بالفحوصات المظهرية والبايوكيميائية. اظهرت (489) عينة ملوثة بالبكتريا وبنسبة | • Five hundreds swabs were collected from patients suffered from burns in three hospitals of Baghdad city, for the period from 1st June 2006 to 1st November 2007. The swabs were implanted in order to be isolated and diagnosed by morphological and biochemical tests. • The results showed that 489 swabs were contaminated with bacteria (97.8%), while 11 swabs were not (2.2%). The identification and prevalence of the bacteria was as follow Pseudomonas aeruginosa was found in 148 swabs (30.27%), Klebsiella pneumoniae in 102 (20.26%), Staphylococcus aureus in 79 (16.16%), Escherichia coli in 70 (14.31%), Proteus mirabilis in 52 (10.63%) and Enterobacter cloacae in 38 (7.77%). • Information card for each patient was assigned included age, sex, time of suffering from burns, social status and address.• The degrees of suffering were as follow : - First degree of burns, 201 cases (40.2%). - Second degree, 187 cases (37.4%). - Third degree, 112 cases (22.4). Sensivity test to antibiotic and was done for all the isolated bacteria, and it was found, that, they were sensitive to Vancomycin, Ciprofloxacin, Amikacin, Tetracyclin and Fusidic acid within the percentage ratio of 0.0, 59.1, 3.7, 58.5 and 11.6%, respectively. Also it was found that the investigated bacteria showed high Vesistance to Penicillin G (87.9%), Erythromycin (93.9%), Trimethoprim (100%), Carbencillin (83.9), Cefotaxime (63.4%), Ampicillin (75.9%), Gentamycin (67.9%), Streptomycin (83.6%), Nalidix acid (89.4%) and Linomycin (83.2%). o Searching for the active compounds in the extracts of investigated plants (Linum usitatissimum, Nigella sativa and Eruca sativa) was conducted. It was found, that, the seeds of the three plants contained Glycosides, Alkalis, Flavonoids, Soapiness and Resins, which are not water soluble, but could be detected by the alcoholic extraction. Cumarin was noticed in the seeds of N. sativa and E. sativa only. o Different concentrations of water and alcohol extracts were prepared in addition to the isolation of oils, soapiness and Flavonids. The effectiveness of the extracts was tested at the concentration of 16, 32, 64, 128, 256 mg/ml for all the plants, against the bacteria.o The Phenol, water and alcohol extracts of L. usitatissimum and E. sativa showed high effect against the bacterial species, while that of N. sativa was less affective.o The Minimum inhibition concentration (MIC), was found to be different according to the type of extract and the species of bacteria.o The effects of the organic acids (Acetic, Citric, and Lactic) were tested at concentrations of 0.5, 1, 1.5, 2, 3 and 4% of each acid. An increase in the diameter of inhibition area, related with the increase in the acid concentrations, was detected. It was found that the acetic acid was more affective against the microorganisms, followed by the Citric acid and then the Lactic acid.o on the base of ratios of inhibition area diameters (in vitro), the results illustrated that E. coli was more affected by Acetic acid when the diameters reached 18.75, 21.75, 30.75, 38.25, 40.00 and 44.75 mm, respectively. The concentration of 1.5% was the effective enough against E. coli as diameters rates for P. mirabilis were 19.25, 18.25, 18.0, 22.56, 27.75 and 31.0 mm, respectively, followed by Ps. aeruginosa, S. aurues and En. cloacae when exposed to the optimum concentrations of Acetic, Citric, Lactic acids. The diameters at concentration of 4% were 23.50 and 23.66 mm. o The water extracts from all the involved plants, in addition to the organic acids showed good levels of effectiveness against the different studied bacterial species, when suffered by burns rats were exposed to them. A clear decrease in bacteria numbers in these rats in comparison with non - treated animals, in the swabs removed from the treated and non - treated rats, in addition to the absence of any histophathalogical changes in livers and skins of the treated animals. o Biochemical tests of serums from animals treated with the water extracts and organic acids at concentration of 16 mg/Kg and 18 mg/Kg for seven days and the microscope investigation, proved that these compounds were not toxic.o The effects of the water extracts and the organic acids were studied too from the point of the immunity point of view. An increase in the Levels of IgG and IgA of the supplementary systems C3 and C4 was noticed in the suffered from burns in general. The levels of IgG and IgA tended to be normal after the treatments with the water extract from the studied plants and with the organic acids.o The L D50 of N. sativa and E. sativa was 18 mg/kg while it was 16 mg/Kg of L. usitatissimum.

دراسة جزيئية عن جين المقاومة mecA في بكتريا العنقودية الذهبية المقاومة للمشيسلين والمعزولة من بعض مستشفيات بغداد Molecular Study For Detection of Meca Gene In Methicillin - Resistant Staphylococcus Aureus Isolated From Some Hospital In Baghdad City

اسم المؤلف: لمى ياسين موسى
اسم المشرف: محمد ابراهيم نادر
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two hundred and thirty clinical specimens were collected from two different hospitals in Baghdad during the period (December 2012 to April 2013). These specimens were collected from visitors, hospitalized patients and the health care workers in these hospitals. The specimens included nasal swab, wound swab, burn swab, abscess and pus, sputum, ear swab, urine and blood culture diagnostic results show that 150 out of the (230) specimens gave positive bacterial cultures and (100) isolates are characterized as Staphylococcus aureus depending on the cultural and biochemical examinations.the coagulase test was performed and the results showed that from total 150 isolates of Staphylococci, 100 isolates (61%) were coagulase - positive (COPS), while only 50 isolates (39%) were coagulase negative (CONS). In addition, the distribution of methicillin resistance among Staphylococcus spp. was investigated.The use of the antibiotic susceptibility profile for these isolates was examined against methicillin resistance. Using disk diffusion method revealed that (13) isolates were proved to be methicillin resistant Staphylococcus aureus (MRSA), While (87) isolates of S. aureus showed sensitivity to methicillin (MSSA) and there was no intermediate resistance among these isolates.The ability of MRSA isolates to produce some virulence factors were investigated and the results showed that MRSA isolates produce many enzymes and toxins that contributed in their virulence such as protease, urease, dnase and gelatinase, and also produce a beta types of haemolysins.The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA were produced slime layer when theytested by Congo red agar method and the results showed that all of MRSA isolates produced strong slime layer.One of the aims of this study was to adopted a accurate diagnostic method to detect S.aureus by its genetic material contents through extracting of DNA and gel electrophoresis of the PCR product for the specific gene.Detection of methicilline - resistance gene represented by A confirmatory test was carried out for the selected isolates using Polymerase chain reaction (PCR) technique for further characterization up to the species level by the amplification of (mecA) gene.This is Staphylococcus aureus specific gene that encodes the extra Penicillin Binding Protein, which is unique to methicillin - resistant staphylococci. All the (13) positive isolates by disk diffusion test are found to be positive for the presence of (mecA) gene as their agarose gel revealed the presence of DNA band of mecA gene with a molecular size about (200 bp.).Results of the detecting (femB) gene showed that it was positive in all of MRSA isolates as they appear to have a band with a molecular size of about (651 bp). The genetic determinants of methicillin resistance mecA and femB genes were amplified using multiplex PCR technique in order to identify methicillin resistant (mecA+) and susceptible (lacking mecA) staphylococci and to differentiate S. aureus (femB+) from coagulase negative staphylococci (lacking femB). All of the S. aureus isolates (100%) were found to harbor femA gene, it is species specific marker for S. aureus.

استجابة ثلاثة اصـناف من حنطة الخبز Triticum aestivum L. باضافة سماد NPKZn والرش بالجبرلين Response of Three Bread Wheat (Triticum Aestivum L.) 3With Gasprying Fertilizer And NPKZn Cultivars To

اسم المؤلف: عبد الجبار جاسم جريان
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A field study was conducted at the farm of Biological Dpartment, College of Education (Ibn Al - Haitham) University of Baghdad during the grown season 2013 - 2014, to study the effect of compound fertilizer NPKZn (15 : 15 : 15 : 1) - Turkish origin was introduced to Iraq recently - in both ) 3(GA acid Gibberellicvarious concentrations of with morphological and chemical indicators, the components of yield, some quality and biological characteristics of three varieties of the Iraqi wheat (Bohoth 22, Alfath, Bohoth158). Experiment was carried out according to the design of complete sectors of randomization [Randomized Complete Block Design (RCBD)].160) (0 of fertilizer, two levels used experiment Asthree replicates, with ) 015, 100, acid concentrations (25, 50 Gibberellicof fourand 1 - .hKg and three varieties of as well as to the treatment of comparison 1 - .lmg wheat. So the expriement was factorial on (2 x 5 x 3).It included 90 experimental units..The study of components of yield have gone on its : 1. There is difference in these three different varieties Bohoth 22, Alfath, and Bohoth 158 in the studied indicators.The class Bohoth 158 exceeds on the other categories in the following phenotypic indicators (dry weight of vegetative part, space of flag leaf and sustainability of biomass), with increase as like {(17.85, 11.37), (14.66, 7.33) and (17.02, 14.24)}%, respectively, while Bohoth 158 significantly exceeds on the other varieties absolute growth rate. And also the differences were significant between varieties in the following chemical indicators (phosphorus and calcium content and the percentage of protein in the grain) and in the components of yield there was significant increase in the (spike length with awn and without its, the number of spikes, weight of spike and its grains, weight of grains in spike and grains yield), while in the Bohoth 158 there was a significant increase in qualitative and rheological characteristics for the following qualities, (the proportion of wet and dry Glutine, flour absorption susceptibility of water, sedimentation values, ash content in coarse bran compared to others two classes. 2. The results of the study showed that the transactions under the influence of fertilizer has excelled significantly in all the study indicators (morphological and chemical components of yield, quality and rheological) with the exception of the dry weight of roots. acid was the best among Gibberellic 1 - .lmgof 100 concentration 3. The the concentrations giving him the highest average of indicators of morphological and physiological components of yield, quality and rheological. and spray 1 - 160 Kg.hbetween the manure level overlap 4. The result Gibberelllic acid showed 1 - mg.l 100 of concentration under the significant increase in all studied indicators except for the (dry weight of roots, the sustainability of biomass, and 1000 - grain weight) 5.The trio overlap transactions showed exceeding of Bohoth 158 nder the influence u 1 - .hKg160 in level fertilizer of add ithw significantly 22 Bohothcategories : other acid onGibberellic of 1 - .lmgof spraying 100 and Alfath in the following indicators : Dry weightof the flag leaf, spike length with awn, number of spikes, grains yield, the percentage of dry Glutine and the values of sedimentation.

دراسة بيئية للدايتومات الملتصقة على بعض النباتات المائية في هور العودة ضمن محافظة ميسان جنوب العراق A Study On The Ecology of Epiphytic Diatom On Some Aquatic Plants In Al - Auda Marshes / Maysan Province / Southern Iraq

اسم المؤلف: الاء عيسى موسى البوعجي
اسم المشرف: جنان شاوي الحساني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الفصلية على الدايتومات الملتصقة على النباتات المائية لثلاث مواقع في هور العودة ضمن محافظة ميسان جنوب العراق (من شهر تشرين الاول 2013 لغاية شهر تموز 2014) نظرا لاهمية الدايتومات في الانظمة البيئية المائية والاهمية البيئية لاهوار العراق على مس | Seasonal study on the quality and quantity of epiphytic Diatoms was conducted in the Al - Auda marsh within Maysan Province throughout one year from October 2013 to July 2014.These Diatoms have an important role in biotic ecosystems. Three taxa of aquatic plants were selected (Phragmites australis Trin ex stand, Ceratophyllum demersum L. and Typha domengensis Pers) in three sites within Al - Auda Marsh; these sites were Al - Adleh, Al - Auda and Um - Almashahef. Also, the study included measuring physical and chemical factors of all the study sites, such as : temperature (air and water), power of hydrogen (pH), total hardness(TH), Calcium (Ca), Magnesium (Mg), turbidity (Tur.), light penetration(LP), depth of water (D), total dissolved suspend material (TDS), total suspended solids (TSS), total alkalinity (TA), dissolved oxygen (DO), total nitrogen (TN) and total phosphorus (TP) and reactive Silicate (SiO3). The mean ranges of the studied factors were recorded : air temperature (10 - 38 C°), water temperature (13 - 34 C°), pH (7.1 - 8.6), TH (672 - 3800mg/l), Ca (97.8 - 264.5 mg/l), Mg (125.1 - 896.13mg/l), Tur.(7.62 - 30.7NTU), LP(46 - 113cm), D (190 - 413cm), TDS (1170 - 3075 mg/l), TSS (0.02 - 0.08 mg/l). while TA (207.5 - 300mg/l), DO (6.4 - 13.5 mg/l), TN (2.41 - 8.58µg/l), TP (0.013 - 0.23 µg/l) and SiO3(101 - 776.77 µg/l).Results of the study indicated presence of 111 taxa of epiphytic diatoms, which belonged to 13 families and 26 genus (one family and two genus of centric diatoms, 12 families and 26 genus of pennate diatoms).The present study recorded 21 species that were found only on one host plant, 20 species that were found on two host plants and 70 species that were found on all host plants, nine of diatoms taxa were present in all host plants among all study seasons which are Achnanthes minutissima, Cocconeis placentula var. euglypta, Cyclotella meneghiniana, Synedra affinis, Syneda ulna , Gomphonem parvulum, Mastogloia smithii var. amphicephala, E. zebra var. porcellus, Rhopalodia gibba.One of the diatoms taxa was recorded in the present study is new to Iraqi flora, the new record of diatom include one taxa of Achnanthidiaceae family (Achnanthes exigua var. constricta). The results of this study included description of the basic characteristics of this taxa with complete photographic documentation.It was evident, from the results, that the morphological form of host plants influenced the number of the attached epiphytic diatoms. C. demersum had attracted 92 diatoms taxa(35%) while the lowest number was 82 taxa (31%) attached by Typha domengensis. The seasonal variation of diatoms growth was evident. Spring 2014 was characterized by the highest number of attached taxa (69 on P. australis - 11%) whereas the lowest number was observed on C. demersum (32 taxa - 5.4%) in autumn 2013.Numbers of epiphytic diatoms fluctuated among study sites, seasons and aquatic plants. The total number of epiphytic diatoms on P. australis ranged between 0.73×104 cell/gm wet weight in summer 2014 at Al - Auda and 406.89104 cell/gm wet weight in spring 2014 at Um - Almashahef. C. demersum recorded highest total number 513.9 ×104 cell/gm wet weight in winter2014 at Al - Adleh, while the lowest total number was 0.1×104 cell/gm of wet weight was recorded in the spring 2014 at Um - Almashahef. While total number of taxa on T. domengensis ranged (0.84 - 427.89) ×104 cell/gm wet weight in summer and winter 2014 at Um - Almashahef.Fluctuations in the total number of main epiphytic diatoms families were observed on all aquatic host plants throughout the study period. The higher total number(92.59×104 cell/gm of wet weight) of diatoms recorded in Achnanthidiaceae family in summer 2014 on P. australis and lower total number (0.28×104 cell/gm of wet weight) on same plant observed in Rhoicospheniaceae family in spring 2014. C. demersum showed highest total number (95.15 ×104 cell/gm of wet weight) in Fragilariaceae family in spring2014, while the lower total number (0.3×104 cell/gm of wet weight) was in family Rhoicospheniaceae in autumn 2013. Individual of Achnanthidiaceae family were recorded as highest total number (168.57×104 cell/gm of wet weight) on T. domengensis in Achnanthidiaceae family in the winter of 2014, but lower total number (0.28×104 cell/gm of wet weight) recorded in Coscinodiscaceae family in the same season. The study showed highest values for richness index (3.77) in the spring of 2014 for epiphytic diatom on C. demersum in Al - Auda site, while lower richness index recorded was (0.6) on T. domengensis in theautumn of 2013 at Um - Almashahef. Shannon - Weaver index ranged (0.24 - 2.45) for epiphytic diatom on P. australis during winter of 2014 at Um - Almashahef and autamn 2013 at Al - Auda, respectively. Highest values for Evenness index were recorded (0.28) for epiphytic diatom on C. demersum in the spring of 2014 at Um - Almashahef site, while for the same site lower values recorded was (0.03) on T. domengensis in winter 2014. Higher percentage for Jaccard similarity index (63.2%) obtained between epiphytic diatoms hosted on T. domengensis and lower percentage of similarity (28.9%) found among the common taxa of epiphytic diatoms on C. demersum, and highest percentage for Jaccard similarity index between host plants was (70%) between P. australis and C. demersum while lower percentage of similarity(67%) was obtained between T. domengensis and C. demersum.

دور الكاينيتين والسماد المركب (NPKZn) في تحمل نبات الفلفل Capsicum annuum L.)) للاجهاد الملحي Role of The Kinetin And NPKZn In Salt Stress Tolerance of Sweet Pepper (Capsicum Annuum L.)

اسم المؤلف: سعاد عبد سيد الجلالي
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجربة اصص في الحديقة النباتية التابعة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم، جامعة بغداد في الموسم الربيعي عام 2014 هدف الدراسة كان دراسة تاثير تركيزين من كلوريد الصوديوم هي 50) و100) مليمول.لتر - 1 فضلا عن معاملة السيطرة واربعة | The aim of the pots experiment which was conducted in the Botanical Garden of Biology Department in the Faculty of Education /Ibn - Al - Haytham / University of Baghdad, during the spring season of 2014 was the influence of different concentrations of Sodium chloride (50, 100) mM.L - 1 in addition to the control treatment and and Four concentrations of kinetin (25, 50, 75, 100) ppm in addition to the control treatment.It is also aiming to study the effect of application and non application the level 160 Kg.H - 1of NPKZn fertilizer, and their interactions in some morphological characteristics root length, root size, plant height, the internode length, stem diameter, Leaf area, dry weight for root and shoot and the absolute growth rate for shoot.and some physiological characteristics Elements content and protein ratio in root, Elements content, protein ratio, total chlorophyll content, carbohydrates percentage and cytokinine concentration in shoot. Biochemical characteristics (enzyme activity of the enzymes) (SOD, POD and CAT) and some antioxidants that nonenzymatic : (the proline and vitamin C concentration) in shoot. Malondialdehyde (MDA) content in shoot, and some Floral morphological, physiological and biochemical characteristics of fruits (Number of branches, The total number of flowers, Number of blooming flowers, ratio of aborted Flowers, Number of knotted flowers, the number and size of fruits, their wet weight , nitrogen and calcium concentration, protein percentage and vitamin C concentration in fruits of pepper plant California weleander variety.The experiment was designed according to Randomized Completely Block Design (RCBD)with three facters kinetin, manure addition and Sodium chloride,) consisting 90 pots. Seedling of pepper plant was planted on 4/2/2014 acompanied by making all agricultural operations of irrigation and the removal of the jungles. results were obtained can be summarized as follows : 1 - The results showed that increasing the concentration of sodium chloride from zero to 100 mM.L - 1 Led to a decline in morphological, physiological and floral characteristics, with and significant increases in the content average of sodium, chloride, The proportion of flowers aborted and biochemical characteristics. The rate quality effectiveness for enzyme SOD increased from 7.24 to 34.66, POD from 8.10 to 26.35 and CAT from 4.91to 39.57 (unite mg protein - 1) , and proline acid content from 23.05 to 40.30 (mg.L - 1) and vitamin C concentration from 2.67 to 3.68 % and Malondialdihyde (MDA) from 1.84 to 3.86 (µM.gm F.W. - 1)2 - As concerns kinetine treatment effect, the results showed a significant increase in the average of morphological, physiological and floral characteristics, and significant decreases in the average of sodium, chloride contents and biochemical characteristics, in the cased increasing in the concentration of kinetine from 0 to 100 ppm caused decreases in the average of quality effectiveness for enzyme SOD from 25.79 to 21.91, POD from 21.83 to 17.20 and CAT from 27.88 to 21.73 (unit mg protein - 1) , and proline acid content from 34.27 to 31.68 (mg.L - 1), Malondialdihyde (MDA) from 3.15 to 2.85 (µM.gm F.W. - 1) and vitamin C concentration from 3.42 to 3.23 %.3 - application the level 160 Kg.H - 1of NPKZn Manure caused a significant increases in all averages morphological, physiological and floral characteristics, and significant decreases in the average of sodium and chloride contents and biochemical characteristics, fertilizing with the level 160 Kg.H - 1of NPKZn fertilizer caused decreases in the average of quality effectiveness for enzyme SOD from 22.03 to 19.88, POD from 17.70 to 14.53 and CAT from 23.34 to 17.34 (unite mg protein - 1) , and proline acid content from 32.84 to 28.93 (mg.L - 1), Malondialdihyde (MDA) from 2.97 to 2. 75 (µM.gm F.W. - 1) and vitamin C concentration from 3.29 to 3.03 %.4 - Results of the bilateral interpenetration between the Fertilization by fertilizer NPKZn and Sodium chloride concentrations, showed that the fertilizations has a positive and effective influence in reducing the negative effects of sodium chloride in all characteristics studied. 5 - The results showed the positive role of kinetine in decreasing the negative effects of sodium chloride Through bilateral overlap between factors. Especially when the concentration of 75 ppm of kinetine and concentration 100 of sodium chloride In all morphological, physiological and biochemical floral characteristics of plant.6 - The results indicated that the Interference between the fertilizer NPKZn and kinetine has a positive and clear role to both factors increase morphological, physiological, floral characteristics and reduce biochemical characteristics.7 - The effect of the triple overlap between study factors was significant, The results showed That plants sprayed kinetine And fertilization led to a significant reduction of most of the negative effects resulting from the of high concentrations of Sodium chloride in all morphological, physiological and flowering traits. Especially in the concentrations 75 ppm kinetine and in the existence of fertilization.

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

اسم المؤلف: التفات فاضل شحاذة الطائي
اسم المشرف: علي هاشم الموسوي كاظم محمد ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

دراسة وراثية جزيئية لبكتريا المكورات المعوية البرازية Enterococcus faecalis المعزولة من جذور الاسنان الملتهبة للانسان في بغداد Molecular Genetics Study of Enterococcus Faecalis Isolated From Root Canal Infection of Human In Bagdad

اسم المؤلف: سوزان علي كاظم
اسم المشرف: عذراء حميد حسون
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم التحري عن نسبة وجود بكتريا المكورات المعوية البرازية Enterococcus faecalis في (100) عينة معزولة من اشخاص مصابين بالتهاب قناة جذر السن, اذ تم جمع (70) عينة من الاصابات الابتدائية لقناة الجذر، و(30) عينة من الاصابات الثانوية لقناة الجذر (اعادة العلاج) وم | To detection Enterococcus faecalis in (100) root canal sample were collected from primary and secondary root canal infection patient`s from all the ages (10 - 50) during the period of (August 2013) till (January 2014). Detection depending on cultural & microscobial characteristics of bacterial cell was done to find (45) 0f E. faecalis species & Biochemical tests & Serological diagnosis by Lancefield method done to find (24) isolates of this species & Diagnosis by Vitek2 was done to find (20) isolates of E. faecalis. When the Molecular genetics Diagnosis was done the result showed find (32) isolates belong to E. faecalis. The antibiotic sensitivity test was done by using (14) antibiotics, (5) isolates showed resistant against all antibiotics & the isolates showed multiresistant against for some antibiotics. All the isolates were resistant by (100 %) against (5) antibiotics. To detection of the isolates ability of production of protease enzyme, lipase enzyme, hemolysin enzyme & gelatinase enzyme.The results showed that (24 isolate) (75 %) were protease producer, & (8 isolates) (25 %) were lipase producer, & (16 isolate) (50 %) were hemolysin producer, & (5 isolates) (15.6 %) were gelatin producer. To detection the presence efa A gene of the isolates by used specific primer to this gene, and all isolates

دراسة تصنيفية مظهرية خارجية لبعض انواع عائلة الفراشات فرشاتية الاقدام من رتبة حرشفية الاجنحة (Lepidoptera : Nymphalidae) في وسط وشمال العراق Morphological Taxonomic Study of Some Species of Brush Footed Butterflies (Lepidoptera : Nymphalidae) In Mid And North of Iraq

اسم المؤلف: ايناس صادق عبد الكريم
اسم المشرف: عماد احمد محمود حسن سعيد الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study comprises taxonomic morphological study of seven species belonging to four genera of the subfamiliesNymphalinae, Danainae, Satyrinae belong to the family Nymphalidae of the order Lepidoptera.The following species were studied in the subject are : Famliy : Nymphalidae Swainson, 1827 - Subfamily : Nymphalinae Swainson, 1827Genus : Vanessa Fabricius, 1807Vanessa japonica Linnaeus, 1758Vanessa cardui Linnaeus, 1758Vanessa atalanta Linnaeus, 1758Genus : Junonia Hubner, 1819Junonia orythia Linnaeus, 1758 - Subfamily : Danainae Swainson, 1827Genus : Danaius Kluk, 1802Danaius chrysippusLinnaeus, 1758 - Subfamily : Satyrinae Boisduval, 1833Genus : Coenonmphyma Hubner, 1819Coenonmphyma pamphilus Linnaeus, 1758 Coenonmphyma spThe species Vanessa japonicaLinnaeus added as new record to the Iraq insect fauna of Lepidopteraand the species Coenonmphyma sp added as new expected species to the world.

العلاقة بين انتاج الانزيم المحلل للكولاجين وتكوين الغشاء الحياتي بوساطة بكتريا Pseudomonas aeruginosae The Relationship Between Collagenase Production And Biofilm Formation By Pseudomonas Aeuroginosa

اسم المؤلف: امال عزيز كريم السعدي
اسم المشرف: شذى سلمان الطحان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A total of 359 samples divided as 228 clinical and 131 non clinical specimens were collected during 2012 from four hospitals in Baghdad city including : Al - Kadhymia Teaching hospital, Baghdad Teaching hospital, The Burn Specialist Hospital and Al - Imam Ali hospital, for isolation of P.aeruginosa to study the correlation between collagenase production and biofilm formation. Eighty two Pseudomonas isolates were screened for biofilm formation, 28 isolates were classified as strong biofilm formers, 25 as moderate and 27 as weak biofilm former. The 28 isolates were identifid by VITEk - 2 Compact system which confirmed that the isolates were P.aeruginosa. Collagenase production assay was used to screen 28 isolates that were strong biofilm formers inorder to detect the ability of these isolates to produce collagenase, the substrate of collagenase (collagen) was purified localy from bovin tendon and the results showed that just 8 isolates could grow in mineral salt media with collagen after 4 days of incubation. The factors affecting biofilm formation and collagenase production were studied to determine the optimual conditions for their production, those factors included : 1 - Nitrogen sources represented higher influence on collagenase production specialy (yeast extract) in media containing collagen than other media without collagen as a substrate. The specific activity differed between the 8 isolates, biofilm formation also became more pronounced with (yeast extract), while NH4Cl and NaNO3 depressed biofilm formation at the same conditions. The statistical analysis between the two parameters (biofilm and collagenase) according to different nitrogen sources demonstrated highly significance at p?0.01 with yeast extract and casein. 2 - pH, results showed that the best pH for production was 7 for both collagenase and biofilm.The statistical analysis for determination the relationshipe between the two parameters showed highly significance at p ?0.01 for different pH. 3 - The maximum production of the two parameters was at 35?C temperature which gave highly significance at p?0.01 with defferent temperature. 4 - Long incubation periods revealed increasing in collagenase production and biofilm formation which represented highly significance detween them when incubation periods were prolonged at p?0.01. Results of this study showed that collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction. The REP - PCR analysis using BOX - primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP - PCR in 9 different genotypes, named cluster 1 to 3 which included C1, C2, C3 with relatedness : 8 (80%), 8 (86%), 3 (80%) respectively. A19 and A20 both of them were not included in any cluster, they have 78% similarity.The REP - PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13), showed similarity reached 86

عزل وتشخيص الجين lipA المنتج من بكتريا الزائفة الزنجارية من مياه الصرف الصناعي Isolation And Identification of lipA Gene Producing Pseudomonas Aeruginosa From Industrial Wastewater

اسم المؤلف: انتصار طه لفتة
اسم المشرف: واثق عباس الدراغي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: توضح هذه الدراسة عزل lipA gene من الزوائف الزنجارية من مياه الصرف الصناعية للزيوت النباتية وتشخيصها اعتمادا على تفاعل البلمرة المتسلسل (PCR). استغرقت هذه الدراسة تسعة اشهر, من شهر شرين الاول 2014 لغاية حزيران 2015. تم جمع خمسين عينة من مياه الصرف الصناع | This study clarify the isolation and identification of lipA gene in Pseudomonas aeruginosa from industerial wastewater of vegetable oils factories depending on the polymerase chain reaction (PCR) technique. The present study had taken nine monthes starting from October 2014, till the mid of June 2015. Fifty samples of industrial wastewater were collected from the factories of the general company of vegetable oils, fourty from AL Rasheed factory and ten from AL ameen factory, the samples collected from the physical, chemical and biological treatment units, and other different wastewater tanks departments. While the four sewage samples were collected from sewer service Baghdad /Alrustumaiya. For Identification of Pseudomonas aeruginosa, two types of tests were used in this study. The first type was the routine tests, which include selective cultures, bacteriological and biochemical tests. Thirty four of fifty samples (68%) gave a positive growth and results for tests that were used to confirm the presence of P. aeruginosa. In addition to diagnosis P. aeruginosa in sewage sample which prepare the biological treatment units in vegetable oils factories as active sludge, other bacterial types have been diagnosed by using traditional methods and API 20 E system. For detection of bacterial lipolytic activity, two methods were used for this purpose, the first was the screening of bacterial lipolytic activity which was based on values of clear zones diameter around bacterial colony. The best values were between (1.9 _ 2.7) cm. The second test was carried by the Gas - Chromatography - Flame ionization detector, fatty acids solutions that were produced from hydrolysis by lipase enzyme were extracted by Petrolium ether solvent then analyzed by gas chromatograph apparatus. The second type of test was the Molecular diagnosis by using the Polymerase Chain Reaction (PCR) to detect lipA gene in Pseudomonas aeruginosa by two primers. Through two primers used, lipA 948 was the best and more specialized primer to isolation lipA gene of P. aeruginosa. It gave (100%) a positive result. While the second primer lipA 558, gave (66.66%) a positive result and that may be due to the design of this oligonucleotides was not specific for lipA of P.aeruginosa. This is so as was based on highly preserved region of 12 bacterial lipA - homologous genes for many genus and many species belong to Pseudomonas. DNA sequencing done for amplicon generated using lipA 948, this sequence aligned by using BLASTn software against NCBI database to validate the results and investigate the similarity degree with other corresponding strains.

الاكثار الخضري لنبات Spilanthes acmella (L.) Murr. خارج الجسم الحي In Vitro Vegetative Propagation of Spilanthes Acmella (L.) Murr

اسم المؤلف: انسام زهير جاسم الحسني
اسم المشرف: بشرى محمد جابر علوش
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: وظفت تقانة زراعة الانسجة لاكثار نبات Spilanthes acmella (L.) Murr. كونه نباتا غير مستزرع في العراق ولاهميته الطبية والزراعية بوصفه نبات زينة فضلا عن استعمالاته الاخرى. عقمت البذور بمادة هايبوكلورات الصوديوم(NaOCl) وزرعت على وسط Murashige وSkoog (1962) (MS | The technique of plant tissue culture has been manipulated to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized by sodium hypochlorite (NaOCl) and cultuared on full strength Murashige and Skoog medium(1962) (MS). Nodal segments, apical shoots, and leaflets explants were taken from seedlings and cultured on (MS) medium enriched with different concentrations of Indol acetic acid (IAA), Naphthalene acetic acid (NAA) Benzyl adenine (BA), and 6 - furfurylaminopurine (Kin.). The study include the effect of node orientation vertically and horizontally on induction of shoots formation. The effect of the interaction between IAA and BA, IAA and Kin. on shoot multiplication was investigated. Rooting was also studied after inclusion of IBA and IAA to full and half - strength (MS) medium. At acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. DNA isolation was carried out from fresh and dry plantlet leaves as well as from seeds and Agarose Gel Electrophoresis (AGE) was done. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. The nodes cultured vertically exhibited relatively highest response as compared with apical shoots and leaflets culture. Supplying the culture medium with 1 mg/L. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding IAA at 0.1 and Kin. at 0.5, 1.0 or 1.5 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 14.20, 13.60 shoots/ explants with a mean length of 3.40, 2.60, 1.40 cm, respectively. Adding 0.1, 0.5, 1.0 mg/L IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots, respectively, with mean length of 3.25, 3.80, 3.80 cm, respectively. Plantlets were acclimatized successfully achieving 100% survival after four weeks when transferred to agricultural medium consisted of river sand and peat moss at 1 : 1 v/v ratio. This study showed the ability of in vitro micropropagation of Spilanthes acmella (L.) Murr. DNA isolation from drying leaves was butter than fresh leaves with the precense of liquid nitrogen.

تاثير الكلوتاثيون وبيروكسيد الهيدروجين وتداخلهما في بعض الصفات النوعية والكمية لنبات الماش Vigna radiat Effect of Glutathione And Hydrogen Peroxide And Their Interactions On Some of The Quantity And Quality Characteristic of (Mung Bean) Plant Vigna Radiata L.

اسم المؤلف: ايمان حسين هادي الحياني
اسم المشرف: وفاق امجد القيسي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments were conducted during the spring and autum growth season of Vigna radiata L. in the year 2014 at botanical garden of Biology Department, Collage of Education for Pure Science (Ibn AL_Haithem) University of Baghdad.The experiments aimed to study the effect of glutathione and hydrogen peroxide, and their interactions on some of quantity and quality characteristic of plant Vigna radiate L.The treatment of glutathione (0, 25, 50, 75, 100) mg.L - 1 While the H2O2 (0, 5, 10, 15) ml mol. L - 1.The two experiment were designed as Randomized Complete Block Design as factorial experiment with two factors with three replication which included 60 experiment units the area of each one (1×1`) m. Results were analyzed Statistically and compared using average L.S.D at 0.05. The results of the experiments showed that glutathione revealed significantly increased all study parameter espically in 100 mg.L - 1 concentration, stem diameter by 54.56%, 28.04%, leaves number 69.34%, 53.14% for two seasons, fresh weight 44.20% for first season, the dry weight 37.43%, 91.46%, leaf area 61.61%, 151.23%.leaf area index 86.61%, 146.35%, biomass duration 60.48%, 101.06%, absolute growth rate (AGR) 32%, 92.68%, inflorescences number 56.55%, 39.90%, flower`s number 71.97%, 22.60%, root length19.82. %, 26.20%, root dry weight 76.10%, 79.25%, total function of SOD 69.32%, 40.52%, total Function of POD 29.45%, 82.25%, total Function of GPX 30.90 %, 63.62%, chlorophyll concentration of a 73.48%91.40%, chlorophyll concentration of b 35.42%, 17.67% for two seasons respectively. The total chlorophyll content 13.69%, for second season, Caroten concentration 207%, 309%, proline concentration 84.47%, 31.75%, (MDA) concentration 6.25%, 38.35%, glutathione. concentration 41.49%, 23.62 %, H2O2 concentration 52.16%, 33.24%, pods number 17.43%, 16.93%, 100 seeds weight 22.95 % 22.48%, , 93.62%, seeds yield 52.17%, 43.70%, the carbohydrate percentage 64.07%, 19.21 %, the protein percentage 22.32% for first season while the results of effect of soaking seeds with H2O2 specially with 15m ml.L - 1 concentration increased stem diameter 45.52%, 34.00%, leaves number 18.55%, 30.75%, Lateral branch 26.06%, 48.04% for Two seasons respectially, fresh weight 35.45% for first season, dry weight 40.89%, 43.85%, leaf area 53.24%, 64.53%, leaf area index 92.30%, 62.39%, biomass duration 35.35%, 57.53%, abosult growth rate34.78 %, 48%, in, inflorescences number 34.98%, 38.10% flowers number 2.987, 20.13%, root length19.44 %, 19.36%, root dry weight 99%, 96.52% total functhion of (SOD)100%, 2853%, total function of (POD) 176.57%, 40.58%, total function of (CAT)118.29%, 71.78%total functhion of (GPX)12.80%, 61.40%, Caroten concentration 54%for second season, prolineconcentration 40.93%for first season, (MDA) content 17.31% for second season, glutathione concentration, 13.68% 24.29%, H2O2 concentration 26.53%, 30.58%, number of pods 42.77%, 20.51%, seads number /pod 22.93%, 22.93%, 100 seeds Wieght 24.07%, 24.86%, seeds yield 16.69% for first season, the solubly carbohydraty percentage 43.26%for first season, the protein percentage 11.50%, 25.18 % for two seasons respicatlly, while chlorophyll a decreas concentration ed by 15m ml.L1 12.87%for second season, total chlorophyll concentration 39.66%, 26.22 for two season respicatlly. There were high significant interaction between glutathione and H2O2 in all study parameter characters in the two experiments.

تاثير الجسيمات النانوية للنحاس على بعض اعضاء الجهاز التكاثري الذكري للفئران البيض Mus musculus Effect of Copper Nanoparticles On Some Organs of Male Reproductive System In Albino Mice Mus Musculus

اسم المؤلف: ايمن راضي حبيب
اسم المشرف: محمد ناجي طه
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study was conducted on 20 male of Swiss albino mice, where the main target of this study was to identify the influence of copper nanoparticles (Cu - NPs) on some organs of the male reproductive system. Animals were orally administered with 0.1 ml of 100 mg/kg Cu - NPs for 7, 14, 21 days. Then the animals were dissected in the next day after the end of the dosing period and took from them testes and epididymis (head and tail) to study the parameters in which these particles have affected, and this parameter as follows : 1 - Changes in the weights of each of the animals bodies, testes, tunica albuginea, and epididymis (heads and tails). 2 - Changes in sperm characteristics in testes and epididymis (head and tail), which included : the percentage of live sperms, abnormal sperms and sperm concentration. 3 - Changes in the percentages of the spermatogenic cells, which included : a - Spermatogonia. b - Primary & Secondary Spermatocytes. c - Spermatids. d - Sperms. 4 - Changes in numbers of leydig cells and nuclei diameters. 5 - Changes in diameters of seminiferous tubules and their lining wall thickness. 6 - Changes in thickness and height of epithelial cells in both the head and tail of the epididymis. 7 - Histopathological changes in testes and epididymis (head and tail). Phenotype description of testes from mice treated with copper nanoparticles for a period of 21 days occurrence of congestion and a few amount of fatty material around the testes. The results also revealed a decline in the average body weights on the period of 7 and 21 days of treatment when compared with the weight of the same animals before treatment and the decline was significantly at (P<0.05). Whereas the testes and tunica albuginea weights recorded a significant decrease in their average weights (P<0.05) when compared with control groups. As well as the epididymis weights where showed significantly decrease (P<0.05) in the average of the epididymides heads weights for all three periods of treatment, while a significant decrease (P<0.05) in the average weight of the epididymis tail appear for two periods 7 and 21 days compared with control groups. The study also reported a decrease in the percentage of live sperm and the average sperm concentration and an increase in the percentage of sperm abnormalities extended to all periods of treatment compared with control groups and this change was significant at the level of (P<0.05). The number of leydig cells and nuclei diameters, the results show a decrease in their averages and all the extended treatment period when compared with control groups, and this decrease was significantly at (P<0.05). Treatment with particles of copper nanoparticle indicated a decrease in the percentage of spermatogonia, primary spermatocytes and sperms in all periods of treatment compared with control groups and this decrease was significantly at the level of (P<0.05). While the results of the study revealed a significant increase at the level of (P<0.05) in the percentage of secondary spermatocytes and spermatids compared with the control animals. The study also showed a significant decrease at the level of (P<0.05) in the diameter of the seminiferous tubules of testes and the wall thickness of the seminiferous tubules for all periods of treatment compared with control groups. As well as a reduction in wall thickness and height of the epithelium in epididymis (head and tail) for all periods of treatment compared with control animals and this decline was significantly at (P<0.05). The current study also recorded a textile damage in the testes and epididymis (head and tail), which summarized the crash of some seminiferous tubules, degeneration of lining cells in tubule, the appearance of vacuole between cells and lifting epithelium from the basement membrane. On this basis, this study has shown for the first time that particles of copper nanoparticles have a negative impact on the effectiveness and activity of the male reproductive system in albino mice.

دراسة تشريحية مقارنة للاجزاء الخضرية وبعض الاجزاء التكاثرية لمراتب من العائلة القرعية Cucurbitaceae في العراق Comparative Anatomical Study of Vegetative And Some Reproductive Parts For Taxa of The Family Cucurbitaceae In Iraq

اسم المؤلف: باسمة محمد رضا كاظم العبيدي
اسم المشرف: عذية ناهي سلمان المشهداني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - التشريح
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية دراسة تشريحية مقارنة لتسعة مراتب من العائلة القرعية Cucurbitaceae المستزرعة والبرية والتي زرعت في الحديقة النباتية لكلية التربية للعلوم الصرفة ابن الهيثم والمراتب هي : 1 - Citrullus colocynthis Schrad2 - Citrullus vulgaris Schrad3 | The present study included comparative anatomical study for nine taxa of Cucurbitaceae family for cultivated and wild types, which were planted in the garden of College of Education for Pure Sciences/ Ibn Al - Haitham. These taxa included : Citrullus colocynthis Schrad, Citrullus vulgaris Schrad, Cucumis melo Linnaeus, Cucumis melo var.flexuosus Naudin, Cucumis sativus Linnaeus, Cucurbita maxima Duchesne, Cucurbita pepo Linnaeus, Lagenaria siceraria (Mol.) Standl, Luffa cylindrica (Linnaeus) M. Roem. This study focused on some the anatomical characterization as compared study for the first time to these taxa in Iraq. This study included the features of ordinary epidermal cells, leaves petioles, stems, pedicles and sepals of the studied taxa. The results found the appearance of stomata in the stems and petioles in straight line as adjacent planar shape to the epidermal cells and not scattered as found in the leaves, this result was seen for the first time in the current taxa. Furthermore, the stomata have been shown in pedicel and sepals in same type as the stomatal complexes in leaves. The importance of phenotypic variation for the length and types of eglandular trichomes and the presence or not of glandular trichomes were found to carry out the role of indumentum in the different isolated taxa. Additionally, this study included the examination of the cross sections of the roots and also exposed the constituent tissues of it, the variation in the thickness of epidermis, cortex, vascular cambium as well as the arrangement of phloem cells have an important role in the studied taxa. The cross sections of stems, leaves petioles and pedicles showed shape and studied a significant role in the studied taxa and split them into groups.Moreover, this study concluded that the following of above sections from the outside into the inside has an important role to find out their constituent tissue. Thus the results of the study clarified the importance of epidermis tissue through the explanation of the outer and inner tangential walls of their cells and the variation in their dimensions, thickness and kind of stomatal complexes; therefore it has been given the importance of those features in the studied taxa in this study. Cortex tissue showed an extension of angular collenchyma and chloronechyma from stem to pedicel and have shown variation in the thickness in these two layers which was useful in the classification of studied taxa. Vascular tissues differed in the shape of vascular bundle, the number of rows and xylem units and the arrangement of the internal phloem, and all of those characteristics have helped in the studied taxa. In addition, the vascular tissues helped to find their arranged in the stems and found as vascular cylinder shape, while in the petioles and pedicel shown as vascular ribbons were which took the shape of sections petioles and pedicel. The medullar cells and medullary rays have been studied and also their similarity in the morphological characterization and function. Moreover, the anatomical features of the vertical sections of the laminae of leaves in mesophyll tissue have been examined, in the thickness of palaside and spongy layers in midrib, the variation in number and arrangement of vascular bundles have an important role in the studied taxa. In the current study, the ordinary and petal leaves venation and the type of venation have been studied in the studied taxa. Moreover the vertical sections for sepals were examined and also their similarity with laminae of leaves which was useful for studying the constituent tissues of some parts such as pedicel and sepals and anatomical features; and all of the studied characterization helped to study the vegetative parts through following them in studied taxa.

التحري عن الاميبا الحالة للنسج/ الاميبا المتغايرة Entamoeba histolytica/Entamoeba dispar في عينات البراز للمرضى با ستعمال تقنية PCR Detection of Entamoeba Histolytic /Entamoeba Dispar In Stool Specimens By Using PCR Technique

اسم المؤلف: بثينة عبد الحمزة حسون الزبيدي
اسم المشرف: اخلاص مشرف عيدان
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تسلط الدراسة الحالية الضوء على تشخيص وتحديد نسبة الاصابة بطفيلي الاميبا المرضية المتمثلة بالاميبا الحالة للنسج Entamoeba histolytica وتمييزها عن الاميبا غير المرضية المتمثلة بالاميبا المتغايرة Entamoeba dispar اللتين تتمايزان بشكلهما المتماثل في كلا الطور | This study sheds light on the determination and diagnose of the percentage of infection of like Entamoeba histolytica and differentiate it from the non pathogenic Entamoeba dispar that were morphologically identical in both cysts and trophozoite phase in two different groups : the first group includes 50 stool samples of patients suffering from diarrhea and abdominal pain (symptomatic group) while the second group includes 95 stool samples of patients not suffering from diarrhea and abdominal pain (Asymptomatic group).In addition to that, 20 stool samples were collected from healthy individuals as a control these samples were collected patients of AL - Zafaranyia General Hospital and AL - Alwyia Childhood Teaching Hospital in Baghdad from July, 2011 to May, 2012.These samples belong to different ages (1 year - < 50 years) of different sexes (males and females). Entamoeba samples were diagnosed by using two methods : the first one is the wet mount as a General Stool Examination(GSE) after macroscopic examination for sample and classifying them according to their consistency (solid, semisolid and liquid) and color (brown and yellow) and presence of blood and mucous in addition to diagnosing them microscopically to detect the presence of pus cells and both phases trophozoite and cyst for Entamoeba histolytica.While the second method that was depended in diagnosis is duplex polymerase chain reaction (dPCR) by using two pairs of genes for detection of their amplification in DNA sample isolated from stool. The first pair is cystein proteinase which represents one of virulent factors in Entamoeba histolytica parasite as a tissue invader. The second pair encodes the surface antigen present on the surfaces of both parasites E. histolytica and E. dispar. It is represented by Actin gene (Act). The results of the wet mount showed that in percentage of infection of both species (E.histolytica/E.dispar) the (54%) of patient symptom and (34.37%) patient a symptom.While results of the specificity and sensitivity showed that in positive sample wet mount negative PCR while negative sample wet mount showed that positive PCR and higher percentage of infection the Entamoeba dispar. While the results of the dPCR showed that in spite of the presence of both species Entamoeba pathogenic (Entamoeba histolytica) and non pathogenic Entamoeba (Entamoeba dispar) , the percentage of infection of Entamoeba histolytica was lower than that of Entamoeba dispar. This was clear by the detection of amplification the Reaction producets (dPCR) by using both genes : Actin &Ehcp5 where only 8 samples were detected and they were found to be positive amplification Ehcp5 and 31 samples were positive amplification in both groups of patients (symptomatic and a symptomatic). The percentage of infection in females was higher than that in males for all ages when using both tests (wet mount & PCR). More over the patients with less than (10) years old had higher percentage of infection in both sexes.In conclusion, it should not be depended on direct wet mount technique for the identification of infected parasite Entamoeba histolytica and differentiating it from Entamoeba dispar because of their high homology. That the molecular examination is the only method that is able to determine the kind of infection with parasite Entamoeba

انتاج انزيم السيليلز من عزلة محلية لبكتريا B167 Streptomyces sp. واستخدامه في انتاج الوقود الحيوي Cellulase Production From Local Isolate of Streptomyces Sp.B167 And Its Application In Biofuel Production

اسم المؤلف: بنان محمود سليمان
اسم المشرف: ناظم حسن حيدر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى انتاج انزيم السليليز من بكتريا الستربتومايسس ودراسة تاثير بعض الظروف الزرعية على انتاج الانزيم; انتاج الوقود الحيوي من المخلفات السليلوزية من خلال التحلل المائي للمخلفات بالاحماض والانزيمات المايكروبية. تم غربلة 74 عزلة لبكتريا St | The current study was aimed to produce biofuel from cellulosic waste material degraded by local isolate Streptomyces. Seventy four isolates of Streptomyces were screened for cellulase production in solid and liquid media. The results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose. Therefore, it was selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the isolate Streptomyces sp. B 167 reached to 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) by supplementation of cellulose liquid medium with 1 % of yeast extract as nitrogen source. Additives of carbon sources like (manitol, glucose, maltose, sucrose and starch) to the process of saccharification did not improve the cellulase productivity. The bioconversion of cellulosic waste to reducing sugar was maximum with Banana peels (77.78 %) followed by the rice husk (75.56 %), orange peels (71.11 %), corn steep peels (60.0 %) and lowest bioconversions (53.33 %) were recorded with sawdust. The degradation of cellulosic waste increased with increasing substrate concentration. Maximum cellulase productivity (3.18 U/ml) and bioconversion (86.1 %) was obtained at 3 % (w/v) of cellulosic waste (Banana peels). Saccharification of cellulosic waste with different treatment methods was studied. The pretreatment of cellulosic waste with 1 % HCl and H2SO4 produces 21 and 15.8 g of reducing sugar / 100 g of cellulosic waste. In comparison, hydrolysis with Streptomyces sp. B 167 enzymes resulted a significantly higher amount of reducing sugar yield (25 g / 100 g cellulosic waste). Further fermentation of cellulosic hydrolysates was performed using Saccharomyces cerevisiae using stationary fermentation condition. Maximum yield of ethanol were (0.30, 0.19 and 0.10 g ethanol / g glucose) observed with Streptomyces sp. B 167 enzymes, HCl and H2SO4 hydrolysates respectively after 48 h of fermentation

التحري عن عدد من المعادن الثقيلة والتلوث البكتيري في بعض الخضروات المعلبة والطازجة المستوردة في مدينة بغداد Determination of Several Heavy Metals And Bacterial Contamination In Some Imported Canned And Fresh Vegetables In Baghdad

اسم المؤلف: حسين خالد نعمان
اسم المشرف: ايثار كامل الميالي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم اخذ ستة وثلاثين عينة من الخضروات المعلبة وخمس عينات من الخضروات الطازجة, وتجميعها من مناطق واسواق مختلفة من مدينة بغداد بشكل عشوائي خلال الفترة من شهر تشرين الاول 2013 الى شهر نيسان, 2014 حيث تضمنت الدراسة التحري عن تراكير بعض العناصر الثقيلة وهي (الرص | In this study, thirty six samples of canned vegetables and five samples of fresh vegetables were collected randomly from different markets of Baghdad city from October 2013 till April 2014. The study included determining of the concentration of some heavy metals (lead, nickel, zinc and iron) by atomic absorption spectrophotometery and the bacterial contamination in samples, also comparing the canned vegetables with fresh vegetables. It was found that the higher concentrations of heavy metals in canned vegetables as the following : lead 1.179 µg/g in olive, nickel 0.908 µg/g in olive, zinc 10.143 µg/g green pea and iron 90.601µg/g in white asparagus. The lowest concentrations were as the following : lead 0.002 µg/g in green asparagus, nickel 0.019 µg/g in mushroom, zinc 0.528 µg/g in white asparagus and iron 4.061 µg/g in green pea. In fresh vegetables, it was found that the highest concentrations of heavy metals were as follows : lead 0.919 µg/g in green pea, nickel 1.108 µg/g in green pea, zinc 4.304 µg/g in mushroom and iron 43.618 µg/g in tomato. The lowest concentrations were as the following : lead recorded 0.011 µg/g in tomato, nickel recorded 0.022 µg/g in tomato, zinc 0.876 µg/g in green pea and iron recorded 11.081 µg/g in green pea. The identification of the bacteria done by some identification tests for gram negative bacteria, the testes include growth on MacConkey agar, growth on EMB agar, IMViC testes and motility test. The identification tests for Gram positive bacteria included growth on manitol salt agar, growth on staph 110 agar and motility test. The bacterial tests ensured that there is no bacterial growth was detected in the canned vegetables, except some samples (white asparagus, green asparagus and mushroom) of December 2013, while it was detected in fresh vegetables. The bacterial species that isolated in these samples were E.coli, Klebsiela pneumoniae, Bacillus cereus and Staphylococcus aureus.

دراسة شكليائية ونسجية للقناة الهضمية في سمكة الخشني Liza abu Heckel.1843 Morphological And Histological Study of The Digestive Tract In Liza Abu (Heckel, 1843)

اسم المؤلف: حنان رعد ضياء حسين
اسم المشرف: وجدان بشير عبد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The morphological and histological structure of digestive tract in one species of Iraqi fish that resides in Iraqi inland fresh water (Liza abu) was studied. In this study used (43) fish were in different lengths, the gut of these fish have removed to study the relationship between the length of gastrointestinal tract and the standard body length as well as for morphological and histological study. The value of relative digestive tract length (RGL) was 2.9. Intestine was the longest part of the digestive tract, the average length of intestine was (32 cm). The shape of the folds of the mucous membrane lining the walls of digestive tract showed some differences in different parts of digestive tract, in esophagus the folds were longitudinal running along the wall and it was straight, unbranched and the mean of their number was (9 - 8) folds. In the stomach the folds were longitudinal and (7 - 6) in their number, they were branched into fingerlings structures. In pyloric caeca, anterior and posterior part of intestine and rectum the folds were fingerlings in shape, unbranched and their number were (20 - 19), (27 - 26), (25 - 23), (19 - 18) respectively. Histological study was shown that the wall of the digestive tract is consist of four major coats, from the inside to the outside they were as follows : Tunica Mucosa which characterized by its clear three parts, epithelial tissue, lamina propria and muscularis mucosa. Epithelial tissue in esophagus was thick stratified squamous epithelium, in stomach was thin simple columnar epithelium and gradually increase in length in each of pyloric caeca, intestine and rectum and it was simple columnar epithelial tissue. Mucous which is secreted in esophagus is made of a large proportion of neutral and acidic mucopolysaccharide, while the mucous secreted in the cardiac stomach consist of a large proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, either, in the pyloric stomach is consist of very small proportion of both neutral and acidic mucopolysaccharide, in pyloric caeca mucous consist of a small proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, and the mucous secreted in an anterior, posterior parts of intestine and rectum consist of small proportion of acidic mucopolysaccharide and large proportion of neutral mucopolysaccharide. Lamina propria consists of connective tissue containing capillaries and lymphoid cells and it represent the basis of the folds, in the cardiac stomach it contains simple tubular glands. Muscularis mucosa characterized by the presence of muscle fibers that are not continuous, the higher thickness of this layer was in pyloric stomach. Tunica Submucosa consists of connective tissue that contains capillaries, the higher thickness of this coat was in the stomach and less thickness of this coat was in the rectum. Tunica Muscularis consists of two layers of smooth muscle fibers, the internal layer has a circular arrangement, the external layer has a longitudinal arrangement in stomach, pyloric caeca, intestine and rectum. either in esophagus tunica muscularis was arranged in inverse manner. The circular and longitudinal muscle fibers in esophagus were skeletal muscle fibers, and they were smooth and non - striated in stomach, pyloric caeca, intestine and rectum. the higher thickness of this coat was in the stomach and less thickness was in the rectum Tunica Serosa is present in all regions of digestive tract.

دراسة بعض متغيرات الخطوط الجلدية للبنان ومجاميع الدم لذكور اطفال التوحد

اسم المؤلف: اسراء جعفر صادق السعدي
اسم المشرف: نصر فرحان عبد الله
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الوراثة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

التاثير التثبيطي لبعض المستخلصات على فعالية انزيم Angiotensin converting enzyme وبعض المؤشرات الحيوية المساهمة في ارتفاع ضغط الدم Inhibitory Effect of Some Plant Extracts On Angiotensin Converting Enzyme Activity And Some Biochemical Marker That Associated With Hypertension

اسم المؤلف: رؤى اياد يوسف
اسم المشرف: غازي منعم عزيز حسن فياض
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study was sought to investigate the inhibitory effect of four crude aqueous plant extracts : Zingiber officinale Roscoe (ginger), Camellia sinensis (Green tea), Olea europaea (Olive) and Hibiscus sabdariffa (Rosella) on key enzyme linked to hypertension, Angiontensin - I Converting enzyme (ACE), and on the oxidant/antioxidants status, lipid profile in vitro and in vivo studies. Study of some biochemical biomarker demonstrated that antioxidant enzyme, oxidant enzyme, liped profile and ACE level for 75 hypertension patients. Antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) were shown to have cross relationship with ACE level in hypertension groups, while lipid profile have positive relationship with ACE level in hypertension groups. ACE activity for the four groups G1, G2, G3, G4 were 19.61±3.97, 21.3 ± 1.95, 28.06 ± 5.34 and 32.74±8.19 ng/ml respectively. From these results we concluded that ACE was associated with hypertension and its modulated by drug or herbal extracts. Angiotensin - I Converting enzyme was extracted from sheep lung with specific activity 0.08 U/mg, then the crude ACE extract was concentrated with sucrose by dialysis with specific activity 0.1 U/mg, purification fold was 1.25.The enzyme was purified partly by ion - exchange chromatography using DEAE - cellulose with specific activity 0.5U/mg, yield 30% and purification fold 6.25. ACE activity was determined using N - [3 - (2 - furyl) acryloyl]L - phenylalanyl glycyl glycine (FA - PGG) as a substrate. Results for in vitro ACE inhibitory activity using plant extracts (ginger, green tea, roselle and olive) showed that the all four crude aqueous plant extracts had inhibitory activity in different values when used in the same concentrations about (1 mg/ml), and ginger extract possessed higher inhibitory activity than other three extracts. The ACE inhibitory potency of the ginger extract was found to be significant (P<0.001) when compared with the standard anti - ACE inhibitor drug (Captopril) at the same concentration. The inhibitory activity of ginger extract with different concentration (25, 50, 100 mg/kg) in L - N - ? - nitro - L - arginine methyl ester (L - NAME) induced hypertensive mice was evaluated. Acute oral administration with L - NAME 50mg/kg.b.w causes a rise in blood pressure in normal mice. Administration of aqueous ginger extract (25, 50 and 100 mg/kg) for 4 weeks in L - NAME treated mice significantly (P<0.05) reduced the mean arterial blood pressure compared with L - NAME animals without treatment, with decreasing the serum levels of ACE; while the activity of superoxide dismutase (SOD) and glutathione peroxidase(GPx) showed a significant elevation in ginger treated L - NAME induced hypertensive mice. The results suggest that ginger extract could prevent the development of high blood pressure induced by L - NAME probably can be attributed to prevent or reduce the oxidation process and the inhibition of physiological processes of a substance L - NAME and so as it contains ginger compounds of polyphenols, which inhibits the activity of the ACE and prevent oxidation of fats and repair System Antioxident. Our study concluded that ginger might act as a natural alternative to better and safer in the prevention of negative impacts and risk factors such as high blood pressure and lipids.

تاثير انزيم SNase المنقى من العنقودي في الغشاء الحياتي للايشيركية القولونية والكليبسيلا الرئوية Impact of Snase Purified From Staphylococcus Aureus On Biofilm of Klebsiella Pneumoniae And Escherichia Coli

اسم المؤلف: هند تحسين ابراهيم
اسم المشرف: حارث جبار فهد المذخوري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: SDS - PAGE showed that a single sharp band with an approximately 16.8 KDa molecular weight has been seen. A matter indicates that the enzyme is consistently pure. PCR technique was applied to approve the existence of nuc gene in S.aureus Nevertheless, only those which depicted positive results on DNase agar harboured nuc gene, as it is specified by single band appearance of nuc at assumed molecular weight (ca. 255 pb) of marker that being used. The current study findings highlighted the participation of SNase purified from S. aureus in significant (P< 0.05) preventing biofilm formation by E. coli and K. pneumoniae compared with untreated controls. Evidently, the inhibitory effect of SNase on biofilm formation is undoubtedly perceived, indicating the degradation of the structural major component of biofilm formation (i.e. extracellular DNA). Results revealed that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; E. coli and K. pneumoniae attached to the uroepithelial cells. 1 Chapter one : Introduction and literature Review 1. Introduction and Literature Review 1.1. Introduction Staphylococcus aureus is a persistent human pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. The pathogenesis of S. aureus infection is a complex process involving a diverse array of secreted and surface - associated virulence determinants that are coordinately expressed at different stages of infection (Loughman et al., 2009). Escherichia coli is a genetically diverse species that causes a variety of infections which fulfill many or all of the proposed criteria for biofilmassociated infections (Kaper et al., 2004). Klebsiella pneumoniae is an opportunistic pathogen responsible for a wide range of nosocomial infections. One important factor associated with virulence in K. pneumoniae is its capacity to adhere to surfaces and form biofilms (Cruz et al., 2012). Bacteria often exist as sessile communities called biofilms which are exquisite structures caused by a genetically programmed developmental process. It is estimated that biofilms are involved in 65% of human bacterial infections, since cells in biofilms are 1000 times more resistant than cells in the planktonic state, making medical treatments fail (Shal? et al., 2011). Extracellular DNA (eDNA) plays a significant role in biofilm formation, as revealed by studies in several bacteria including E. coli (Nakao, 2012) and K. pneumoniae (Whitchurch et al., 2002); however, there is no definite proof on a cause - and - effect relationship between DNA release and biofilm formation (Beenken et al., 2012) or becomes a key component of the macromolecular scaffold in many different biofilms (Jakubovics et al., 2013). In rare cases eDNA has been shown to inhibit bacterial settlement. Therefore, it is possible that nuclease mediated eDNA degradation would therefore promote adhesion. Consequently, it is not clear whether microbial nucleases contribute to the gross biofilm structure in clinically relevant situations (Sheilds et al., 2013). 2 Chapter one : Introduction and literature Review All previous studies used both commercial bovine and recombinant human DNaseI in the disruption of medically important biofilms; whilst, extracellular nuclease of S. aureus (SNase) was used against biofilm of S. aureus themselves (Benenken et al., 2012b ; Kiedrowski et al., 2014). Thus, to date, the role of S. aureus exonucleases in biofilm of other bacteria remains unclear. However, in the present study SNase is used against biofilm of other bacterial species (viz. K. pneumoniae and E. coli). To address this, the following steps were undertaken : 1 - Investigating the negative impact of SNase extracted from S. aureus on K. pneumoniae and E.coli biofilm formation. 2 - Studying the preventive activity of SNase on the adherence of K. pneumoniae and E. coli on uroepithelial cell.

دراسة بيئية لبعض انواع صنف بطنية القدم الارضية في ثلاث محافظات وسط العراق An Ecological Study For Some Terrestrial Gastropoda Species In Three Provinces Middle Iraq

اسم المؤلف: ايمان حسين عبد
اسم المشرف: عماد الدين عبد الهادي المختار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية للتعرف على انواع صنف بطنية القدم الارضية المتواجدة في بعض محافظات وسط العراق (بغداد, بابل, كربلاء). اذ تم تسجيل الملاحظات الحياتية والتعرف على بيئاتها المفضلة وقياس العوامل البيئية ذات الاهمية. حيث تم تشخيص ثلاثة انواع وهي : Cornu as | The present study was conducted to identify the terresterial gastropoda species located in some provinces of middle Iraq (Baghdad, Babylon and Karbala). They were record Biological observations on their environment and learn about their prefer environments and to measure the important ecological factors. Three species were diagnosed : Cornu aspersum specie belong to Helicidae family, Monacha cantiana and Candidula gigaxii species belong to Hygromiidae family. Monthly sampling was conducted from (October 2013 - July 2014) of the three provincs on two sites per each provinc, Where the samples were collected randomly by five repeaters of one square meter once a month. The study also included soil ecological properties measuring : soil temperature, soil pH, Electrical conductivity, percentage moisture, organic matter content and soil texture.The results showed soil temperature values ranged between (8 - 30) C?, soil pH (7.33 - 7.96), Electrical conductivity (0.7 - 4.1) ds/m, percentage moisture (9 - 33) % , organic matter content (0.81 - 2.88) %. The results showed percentages rate for clay, silt and sand for Jadiriyah (38.39, 49.18, 12.43)%, Zafaraniya (36.66, 51.53, 11.81)%, Alexandria (37.93, 52.54, 9.53)%, Hindyia dam (40.54, 53.05, 6.41)%, Hosseinie (36.28, 45.81, 17.91)% and Twerij (37.79, 50.20, 12.01)%. Generally soil texture clay silt sandy soil for all study sites. The relative abundance of the studied species was recorded, The lowest value for Candidula gigaxii specie was 7.3 % in March in Hindyia dam, while the highest value was recorded for Cornu aspersum specie 75.0 % in December 2013 in Jadiriyah. Rates of population density (individuals / m 2) were calculated for the studied specimens and the results showed the presence of significant differences when comparing the study sites during research period. The population density rates for species the results showed the lowest value for Candidula gigaxii specie 0.4 (individual/m²) in October 2013 in Jadiriyah, and the highest value for Cornu aspersum specie 19.4 (individual/m²) in Janeuary 2014 in Jadiriyah. The results showed that Candidula gigaxii specie is found in sites (Jadiriyah, Zafaraniya, Hindyia dam(along research period, While the results did not record the presence of it in sites (Alexandria, Hosseinie, Twerij).The study showed presence of positive and negative significant relationships between species and soil ecological properties, as it were for Cornu aspersum specie negative significant relationship with air and soil temperature (0.827, 0.763) respectively, positive significant relationship with moisture and organic matter content (0.703, 0.880) respectively, while the results did not record significant differences with pH and Electrical conductivity (0.096, 0.168) respectively. Monacha cantiana specie has recorded negative significant relationship with air and soil temperature (0.431, 0.553) respectively, positive significant relationship with percentage moisture and organic matter content (0.368, 0.550) respectively, while did not record significant differences for pH and Electrical conductivity (0.214, 0.096) respectively. The results showed for Candidula gigaxii specie negative significant relationship of air and soil temperature (0.504, 0.322) respectively, positive significant relationship with moisture and organic matter content (0.380, 0.569) respectively, While did not record significant differences with pH and Electrical conductivity (0.088, 0.170) respectively.

تحديد تعبير الجين المتحمل للملوحة TaGSK1 في عدد من اصناف الحنـطـة Determination of Gene Expression of Salt Tolerant Gene Tagsk1 In Wheat Cultivars

اسم المؤلف: ايمان نعمان اسماعيل
اسم المشرف: نورية عبد الحسين علي مجيد ارشيد سباح
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت الدراسة الحالية في مركز بحوث التقنيات الاحيائية/ جامعة النهرين للمدة 2011 - 2012 لغرض الكشف عن جين الملوحة TaGSK1 ودراسة التعبير الجيني له في صنفين من الحنطة فرات ودجلة والتركيب الوراثي 2H المنتخبة لصفة تحمل الملوحة مقارنة مع الصنف تموز2 الحساس للملو | This study was conducted in biotechnology research center, Al - Nahrain university in 2011 - 2012 to detect the gene responsible for salt tolerant (TaGSKI) and study its expression in two wheat (Tigris and Euphrates) cultivars and the genotype (2H) were selected for salt tolerance through improvement and breeding programs as compared with to the sensitive local wheat cultivar (Tamooze 2). Seeds of the these cultivars were sown in flasks that contained Ms media in three salt levels (0, 15, 25) ds/m with three replication for each. Five seeds from each cultivars were sown in each replicate. After 10 - 15 days from the sowing, the percentage of germination was estimated, and samples of leaves were collected for RNA extraction and then changed to the cDNA. B - actin gene that consider endogenous control and salt tolerant gene TaGSK1 were amplificated by using PCR technique to discover the gene and by QPCR to estimate gene expression by determine the CT (Cycle Threshold) value for B - actin and TaGSK1 genes in wheat plants of the studying cultivars. Number of DNA dilutions of 189bp molecular weight that extracted from agarose gel were used to determing CT value for each dilution. Standard curves were drawn to find out value of PCR Efficiency which was used for gene expression for the salt tolerant gene TaGSK1.The results of germination percentage (%) showed that there were high differences between the two salt tolerant cultivars (Tigris and Euphrates) and 2H genotype and local cultivar (Tamooze 2). Euphrates cultivar gave 100% germination percentage, and the Tigris cultivar and 2H genotype gave 89, 86% germination percentage respectively at 25ds/m. whilst the local cultivar gave zero germination percentage at the same level of salinity. At 15ds/m level, also the Euphrates cultivar gave 100% germination percentage while the Tigris cultivar and 2H genotype gave 96, 94% germination percentage respectively as compared to Tamooze cultivar that gave 13% germination percentage. All the cultivars have 100% germination percentage at 0.0ds/m level. The conclusion of this result is the two cultivars (Tigris and Euphrates) and 2H genotype more salt tolerance than local cultivar at this growth stage which more salt sensitive than others growth stages. The results of PCR reaction were also indicated that Tigris and Euphrates cultivars and 2H genotype have salt tolerant gene TaGSK1, while this gene did not exist in the local cultivar. There were two bands of TaGSK1 gene that have 189bp and 404bp molecular weight in the Tigris and Euphrates cultivar and the genotype 2H, while the local cultivars have only one band that have 404bp molecular weight. These results were indicated that 189bp molecular weight of this gene is responsible for salt tolerance character in these cultivars.The results of QPCR reaction also were revealed that there is difference between the cultivars in their gene expression. Tigris and Euphrates cultivars and 2H genotype gave the highest gene expression at 15ds/m and increased at 25ds/m as compared to 0.0ds/m. At the second level 15ds/m the gene expression of the two cultivars and genotype was 0.8682, 0.8190 and 0.8688 respectively, and at the third level 25ds/m was 1.656, 1.3176 and 1.2665 respectively, while at the first level 0.0ds/m was less than the other 15, 25 ds/m as compared to local cultivar that the gene salt tolerant (TaGSK1) have no gene expression at the same salt levels. This result indicated that the local cultivar does not have salt tolerant gene TaGSK1. From these results we can revealed that TaGSK1 gene was found in the Tigris and Euphrates cultivars and 2H genotype, and this gene can be considered from high salt tolerant gene, because it gave the highest gene expression at the highest salt level 25ds/m. Therefore this gene help the plant to tolerate salt stress and grow very well. The results also showed that the two salt tolerant cultivars (Tigris and Euphrates) and the salt tolerant genotype (2H) proximately have the same salt tolerance degree, so they have proximately the same gene expression as compared to salt sensitive local cultivar which have no salt tolerant gene TaGSK1. This gene is good indicator for salt tolerance at high salinity levels 15, 25 ds/m in these cultivars and genotype.

التاثيرات المرضية النسجية لعقار ليفيتيراسيتام في التكوين الجنيني لكلى اجنة ومواليد وحوامل الجرذ الابيضAlbino rats (Rattus rattus) Histopathological Effect of Levetiracetam Drug On The Kidney Development In Rats Embryo, Newborns And Pregnant Albino Rat (Rattus Rattus)

اسم المؤلف: لمياء هادي محمد الابراهيمي
اسم المشرف: نهلة عبد الرضا البكري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الاجنة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الصرع هو حالة مزمنة تتميز بنوبات متكررة دون سابق انذار، سببها خلل في نقل الاشارات الكهربائية داخل الدماغ، وعقار Levetiracetam (LEV) من العقاقير المضادة للصرع، ذو خصائص دوائية ملائمة وتظهر فعاليته في تحسين السيطرة على النوبات، ويكون امتصاصه في البلازما سري | Epilepsy is a condition characterized by recurrent abrupt seizures episodes caused by the defect in the transmission of electrical signals to the brain. LEV is one of antiepileptic drugs which has appropriate pharmacological properties and show its effectiveness in improving the control of the epileptic seizers, its rapid absorbance, and its excretion through the kidneys by glomerular filtration and it is re - absorption into the tubular portion.Kidney arise from intermediate mesoderm and it consists of three execratory organs in all vertebrates : pronephros which is transient and present in all vertebrates, mesonephros which also present in all vertebrates embryos and their activity depends upon the type of embryo, and metanephros which is the functional kidney during life after the embryogenesis in reptiles, birds and mammals. The first signals for kidney initiation in albino rat embryo take place in the 10th days of pregnancy. The current study is designed to demonstrate the effect of LEV (350 mg/kg/day) on the kidney development of Albino rat fetus from the age of 8 - 18 days of pregnancy and its effect on the kidneys of the 3 and 7 days aged newborns. In addition to its effect on pregnant rat kidneys treated with the drug for 17 days. Hundred pregnant Albino rat (Rattus rattus) are enrolled in this study, classified blindly to 50 control and 50 pregnant rats are orally treated with LEV (350 mg/kg/day). The histological results of the current study revealed that there is no effect on embryonic kidney in fetuses aged 9 days of pregnancy, whereas, the pronephros of the fetuses aged 10 days shows death of the cells in comparison to control group. Moreover, the kidney of the 11th aged fetuses showed degeneration of the mesonephric duct represented by decrease cellular mass, death and hypertrophy of some cells comparing to the cells of control group. Concerning the kidney of the 12th aged fetuses and subsequent days, there is degeneration of the epithelial cells of the metanephros duct, their detachment from the basement membrane and their congestion in comparison to the cells of the control group. The kidney of the 13th aged fetuses showed swelling, damage for some of the epithelial cells of the glomerulus and collecting tubules, accumulation and shrinkage of glomerular cells, damage, death, and detachment of some collecting tubules cells demonstrated in the 14th aged fetuses. There enlargement of capsular space in addition to the most previous defects was seen in the 15th aged fetuses in comparison to the control group. Most of the previous defects are seen in the 16th, 17th and 18th age fetuses. Scanning electron microscope revealed nuclear enlargement of the podocytes found in the visceral layer of the renal corpuscle of the 16th aged fetuses (mean diameter 64.6 µm compare to 39.2 µm of the control group). Regarding the kidney of 3rd and 7th aged newborns, the histological defects seen in the latest aged fetuses was also seen and there was statistically significant differences (p?0.05) between the diameters mean of the glomerulus of the 3rd and 7th aged newborn. Whereas, there was no significant difference in the diameter of the kidney tubules of 3rd but there was significant difference of the 7th aged newborn. Regarding the kidney of the 17th day treated pregnant rat, there was enlargement of the capsular space caused by accumulation, and degeneration of glomerular cells characterized by loss of the nucleus, shrinkage of the glomeruli, and atrophy of most of them. Loss of the nucleus and detachment of the cells, and swelling of most of the collecting tubules. There was a statistically significant difference between the diameter of the kidney tubules of the pregnant rat and the control group. In conclusion, LEV had histological effect on the kidney of the 10 - 18 aged fetuses and on 3, 7 days aged newborns in addition to its effect on the kidney of the pregnant female treated with the drug for 17 days.

التحري عن بعـض عوامل الفوعة لبكتريا المكورات Enterococci المعوية المقاومة للمضاد الحيوي الفانكومايسين Detection of Some Virulence Factors of Vancomycin - Resistant Enterococci

اسم المؤلف: حيدر صباح كاظم الخماسي
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم الحصول على (20) عزله لبكتريا المكورات المعوية البرازية من اصل (135) عينة جمعت من مستشفى مدينة الطب ومستشفى الكندي وهي كما ياتي (15 عينة الادرار, 60 عينة دم, 50 عينة من قنوات الجذر للاسنان و10 عينة من الحروق).اختبرت الحساسية لهذه العزلات العشرون لـ 11 | Clinical sampling was carried out between September and December 2013, Twenty Enterococcus faecalis isolates were obtained from 135 clinical specimens. The samples included of patients in Medical City Hospital and Al - kindy Hospital (15 urine, 60 blood, 50 root canal and 10 wound swabs) Antibiotics susceptibility test for 20 isolates was done against 11 antibiotics, it was revealed that the isolates showed multi drug resistance were (18) isolates. The vancomycin susceptibility was determined by the minimum inhibitory concentration (MIC). Resistant and intermediate resistant to vancomycin was distributed among isolates at a ratio of 65% and 20%, respectively. Imipenem was found to be the most bactericidal agent against E. faecalis isolates E. faecalis virulence factors were detected phenotypically, The results showed that all isolates (100%) were hemolysin, protease and aggregation substance producer. 30% of isolates showed an ability to produce gelatinase. While (40.7%) of the isolates were a lipase producer. The results of the tube method showed that all E. faecalis isolates (100%) were slime layer and biofilm producer but the amount of adherent layer were different among the isolates ranged from strong to moderate and weak.The extracted DNA was subjected to Polymerase chain reaction (PCR) technique in a monoplex pattern to amplify the virulence factor Enterococcal Surface Protein (esp) which is chromosomal, results of this investigation showed that 20 (100%) E. faecalis isolates gave the amplicon size 933 base pair for the esp gene.The genetic determinants of Vancomycin - Resistant vanA and vanB genes were amplified using monoplex and multiplex PCR techniques in order to identify vancomycin resistant (van+) and sensitive (lacking van) among (13) E. faecalis. The vanA, vanB genes were detected in 11 and 4 E. faecalis isolates, respectively. The results of monoplex and multiplex PCR revealed that the molecular weight of vanA and vanB genes were 550 and nearly 600 bp, respectively. The results revealed that the vanA and vanB amplicons have a genetic variation in their molecular weight during the electrophoresis of PCR product.

التحري عن الطفرات في جيني CNTNAP2 وIL1RAPL1 في مرضى التوحد Mutation Screening of CNTNAP2 And IL1RAPL1 Genes In Autistic Patients

اسم المؤلف: بشير كاظم خرميط
اسم المشرف: عبد الكريم عبد الرزاق القزاز
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان اضطرابات طيف التوحد هي مجموعة من الظروف التي تتميز بضعف في التواصل الاجتماعي ونمطية في السلوك. يختلف الاشخاص المتوحدين اختلافا كبيرا في التطور المعرفي والتي يمكن ان تتراوح من فوق المتوسط الى العجز في التفكير. رغم ان اضطرابات طيف التوحد هي تورث بشكل كبي | The autism spectrum disorders (ASDs) are a group of conditions characterized by impairments in reciprocal social interaction and communication, and the presence of restricted and repetitive behaviors. Individuals with an ASD vary greatly in cognitive development, which can range from above average to intellectual disability (ID). While ASDs are known to be highly heritable (~90%), the underlying genetic determinants are still largely unknown. The research studies correlate between Contactin - associated Protein - Like 2 (CNTNAP2), Interleukin - 1 Receptor Accessory Protein - Like1 (IL1RAPL1) genes and ASDs. This study includes forty autistic patients and forty non autistic children as control groups (twenty unaffected sibling and twenty unrelated children). The age of autistic and non autistic children was ranged from 3 to 10 years. Blood samples of autistic patients were collected from Rahman specialist centre for the care and service autistic children in Baghdad. DNA was extracted from blood samples for molecular detection of CNTNAP2 and IL1RAPL1 mutations associated with ASDs by the use Polymerase Chain Reaction (PCR) technique and sequence analysis. PCR reaction was performed to amplify exons (14, 17 and 20) of CNTNAP2 gene that encode to CASPR2, a member of the neurexin family which functions in the nervous system as cell adhesion molecules and receptors. The PCR results revealed that identical bands related to the CNTNAP2 exons were present in all samples. Therefore, five samples (four from autistic patients and one from control sibling) were selected for genotype analysis of CNTNAP2 exons (14, 17 and 20) by direct sequencing. Genotype analysis revealed that there were no any variants in CNTNAP2 exons, but it shows that four different mutations were identified in non coding region (introns) of the CNTNAP2 gene. These mutations were seen only in autistic patients but absent in control sample. Three of these mutations are single nucleotide polymorphisms (SNPs) (rs3779031 A/G in 2118282 position, rs3779032 A/C in 2118436 position and T/G in 2117905 position). The other mutations were deletion in one nucleotide (Del A/ - in 2117901 position). SNP rs3779032 A/C are located at intron 21 while other mutations are located at intron 19. The current study showed that two common SNPs (rs3779031 and rs3779032) in CNTNAP2 were strongly associated with ASDs, where the frequencies of these SNPs were relatively high. SNP rs3779031 identified in two autistic patients while rs3779032 identified in three autistic patients from four unrelated families with ASDs. PCR reaction also was performed to amplify exons (3, 4, 5, 6, 7, 8 and 9) of IL1RAPL1, a gene implicated in calcium - regulated vesicle release and dendrite differentiation. The PCR results show a large intrgenic deletion (Deletion of exons 3 and 4) in six autistic patients, two of these patients were twin. This deletion may be incomplete penetrance due to phenotypic heterogeneity of these patients. This study provides evidence of the role of genetic factors in the etiology of ASD and the important CNTNAP2 and IL1RAPL1 genes mutation of pathogencity ASDs.

الازالة الحيوية لليورانيوم والسيزيوم من الترب الملوثة بواسطة نبات الشعير Phytoextraction of Uranium And Cesium From Contaminated Soil By Hordeum Vulgare Plants

اسم المؤلف: سيف صبار كامل
اسم المشرف: ناظم حسن حيدر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Phytoremediation is defined as the use of green plants to remove pollutants from the environment or to render them harmless. Phytoremediation process can be classified based on the contaminant fate as; Phytoextraction, Phytotransformation, Phytostabilization, Phytodegradation, Rhizofiltration. In this work, the phytoextraction process was employed. A piece of land in the Botanical Garden at the University of Baghdad with an area of 25 m2 was prepared to plant the barley plant. Then, seeds of barley of type "Samir", which is an Iraqi kind that suitable for cultivation in Iraq, have been seeded. For U and Cs experiments, soil was mixed with a limited quantity of each U isotope for three different concentrations; 152 Bq/kg, 95 Bq/kg and 81 Bq/kg and for Cs106.5 Bq/kg, 79 Bq/kg and51 Bq/kg. For NPK and Urea experiments, different concentrations were tested to establish the requirements of these experiments. The LB4100 - W counting system includes the most complete data analysis software package was used to measure and calculate the alpha and beta concentrations and subsequently the overall activity concentration of any studied sample. Samples were prepared by following the Indian Standard method. For U, the experiment achieved by dividing it into four groups that differ in the spent time of agriculture in contaminated and clean (reference) soils. The results illustrated that the phytoextracted of U with planted period in contaminated soil, which were 31, 50, 63, 34 days, were 36.22, 54.84, 76.24, and 66.30 Bq/kgm, respectively. However, the 4th group differs comparing with other groups in the spent time of cleaned soil, which was 73 days. For Cs experiments, the work grouped similar to U experiment. The results of Cs phytoextraction showed that the absorbed Cs were 54.34, 100.69 and 109.07 for spent times in contaminated soil; 23, 43 and 57 respectively. Furthermore, barley plant has significant ability to phytoextract U and good ability to phytoextract Cs for all the three different concentrations. Besides, the results illustrated that the increase in the planted time in contaminated soils led to increase the quantity of phytoextracted isotopes. The results of adding K fertilizer showed a decrease in the ability of barley to absorb U, while the addition of urea enhanced the ability of barley. Finally, the following conclusion can be drawn from the present study that : barley is a good tool to phytoextract Cs rather than U and the use of urea fertilizer is suitable for enhanced the phytoextraction process.

الكشف الجزيئي عن التغيرات في جين MSX1 المسؤول عن حالة فقدان الاسنان باستخدام سلسة تفاعل البلمرة في عينة من المرضى العراقيين Molecular Detection of Msx1 Gene Changes Responsible For Causing Hypodontia Using Polymerase Chain Reaction (PCR) In Sample of Iraqi Patients

اسم المؤلف: اماني احسان الصقر
اسم المشرف: اسماعيل حسين عزيز اكرم فيصل الحويزي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: مصطلح الهايبودونشيا يعني نقص الاسنان الخلقي ويعد من اكثر التشوهات الفموية حدوثا لدى الانسان. مائة وخمسة وسبعون من مرضى نقص الاسنان الخلقي سجلوا في هذه الدراسة لديهم على الاقل سن واحد مفقود ولايزيد عدد الاسنان المفقودة عن ستة، قصدوعلاج الاسنان التقويمي في | Hypodontia means congenitally missing teeth, and considers as one of the most common oral alteration in human. One hundred and seventy five of hypodontia patients were matriculated in this study, having at least one missing tooth and no more than 6 missing teeth, seeking orthodontic treatment, who attended Al - Huwaizi Dental Center at AL - Harethia District - Baghdad, and control group consists of twenty five apparently healthy subjects (15 females, and 10 males). The age of both groups ranged from14 to 65 year. Buccal swabs were collected (for molecular study) from 25 of those patients who attended the clinic in a period between the beginnings of October 2013 to the end of April 2014, and from control group. Information were taken from the subjects under study according to a questionnaire that includes, name, gender, age, family and medical history, and the place of residence. Hypodontia was diagnosed according to the history of patients, clinical examination, orthopantomogram (OPG), and dental casts. The result of demographic study of hypodontia patients revealed that hypodontia was found in 129 of females which were more than that in males(46) with significant difference (p < 0.05). The ratio of females to males was 2.8 : 1. The missing teeth in hypodontia patients were found in right, left or both sides. The number of hypodontia patients, who had missing teeth in the right side were 37, in the left side were 48, while in both sides reached to 90 with non - significant differences and the results clarified that the missing teeth in anterior region found in 81 patients were higher than that in posterior region (63) while the least in both regions which recorded in 31 patient. It was found that hypodontia was more common in the maxilla (73) (upper jaw) than that in mandible (65) (lower jaw), whereas 37 suffering from missing teeth in both jaws, with non - significant differences. Present results showed that the maxillary lateral incisor (LI) was the most frequently missing tooth (124), the second most missing tooth was mandibular second premolar (PM2) (101) followed by lower central incisor (CI) (33), the maxillary second premolar(PM2) (27) whereas the lowest frequently missing teeth were canine (C), and the first premolar (PM1). The molecular part of present study used polymerease chain reaction (PCR) technique for amplification of DNA samples extracted from buccal swabs of twenty five hypodontia patients and control group.Four pairs of primers X1.1F, X1.3R; X1.4F, X1.4R; X2.1F, X2.3R, and X2.3F, X2.4R of the MSX1 gene, obtained from Bioneer Company (Korea), were used to amplify overlapping regions of the 2 exons of the MXS1 gene. The first pair of primers was used to amplify fragment with product size of 421 bp., while the second, third, and forth pairs of primers were used to amplify fragments with product size of 152 bp., 493 bp., and 264 bp., respectively. The outcome of MSX1 gene amplification showed that four patients with the first pair of primers and nineteen patient with the third pair of primers gave negative result (no bands) which differed from the result of the other patients and control. The disappearance of bands may be attributed to MSX1 microdeletion in those patients.The sequencing of MSX1 gene for the PCR product of second, third and fourth pairs of primer showed no genetic mutation, while the PCR product of the first pair of primers reveled nine missense and two silent mutations.It was concluded that hypodontia occurre frequently in Iraqi population and its occurrence in females was higher than that in males, and the gene MSX1 is responsible for many teeth missing in hypodontia patients.

دراسة نسجية وكيمياء نسجية للقناة الهضمية في طائر فاختة النخيل Streptopelia senegalensis Histological And Histochemical Study of The Alimentary Canal In Laughing Dove Streptopelia Senegalensis

اسم المؤلف: خولة خلف حسون صادق
اسم المشرف: مختار خميس محمد سعيد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تمثل الدراسة الحالية دراسة التركيب المظهري والنسجي وكيميائية النسيج للقناة الهضمية للحمام البري فاختة النخيل Streptopelia senegalensis, لثمان عينات من النماذج الحية البالغة من الحمام البري التي تم اصطيادها حية باطوال مختلفة, وبعد تخديرها بمادة الكلورفورم, | The present study represent the Anatomical, Histological and Histochemical structures of the alimentary canal in the laughing dove Streptopelia senegalensis, for eight adult specimens of these wild birds were trapped alive in different lengths, The specimens were anaesthetized by chloroform, and then carefully dissected for studying the gross anatomy, the alimentary canal from the proximal esophagus to the cloaca was carefully removed from the body cavity, lengths have been taken for alimentary canal and for each part of it, the alimentary canal divided into small pieces for each part, then fixated with Bouin's solution for general histological studies.Transverse sections of the different studied samples were cut at thickness of 5 - 7microns and the prepared sections were stained with routine stains Haematoxylin and Eosina and special stains for histochemical studies ( (Periodic acid - Schiff (PAS) technique, Alcian blue - PAS method and the Mercury bromophenol blue) ). The present Anatomical study showed the esophagus as long tubular organ, the cervical part of it is longer than the thoracic part and the crop was merely enlargement sac in shape which locate at the entrance of the thorax. The stomach is differentiated into a glandular proventriculus and a muscular ventriculus or gizzard.The small intestine distinguish into duodenum, jejunum and ileum which is the longest part of it, whereas the caeca are very small pair, and the rectum is short tube which end with cloaca, the last part of the alimentary tract. The histological study revealed that the alimentary tract composed of usual four layers; tunica mucosa, tunica sub mucosa, tunica muscular and tunica adventitia or serosa. The mucosal layer of esophagus was arranged in folds which was longer in the cervical part and constituted by nonkertenized stratified squamous epithelium, esophageal glands found just in submucosal tunica of the thoracic part. The crop has the same histological structure as in esophagus but it’s folds are less high than them in esophagus and there is no glands on it. The superior gastric glands of proventriculus were simple tubular glands and the deep gastric glands were compound alveoli glands which involve the submucosa tunica.A thick layer of koilen covers the mucosa of the ventriculus it’s lamina propria had loose connective tissue with simple alveolar glands. The villi of small intestine become shorter toward the rectum, which lining by simple columnar epithelium with striated border, and contain goblet cells which gradually increase in number frequently from the duodenum to the rectum. The histochemical studies revealed that PAS and alcian blue / PAS stains were given positive react in the esophageal glands, mucosa layer of esophagus, crop, stomach, and superior gastric glands and the goblet cells of small intestine and rectum. whereas the alcian blue / PAS were givin weak react in deep gastric glands in the provetriculus and gastric gland of gizzard but goblet cells were givin positive react in small intestine and rectum.Proteins were observed in the mucosa layer of esophagus, glandular stomach, intestine and rectum, when they had taken positive react with Mercuric bromophenol blue stain, but the reaction appeared weak in esophagea glands, intestinal glands and goblet cells.

تاثير الدقائق النانوية للفضة في بعض الجوانب التركيبية والوظيفية للجهاز التناسلي الذكري للفئران Effect of Silver Nanoparticles On Some Structural And Functional Parameters of Male Reproductive System of Mice

اسم المؤلف: امير محمد جعفر علي حسين البلداوي
اسم المشرف: محمد ناجي طه
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تطرقت هذه الدراسة الى معرفة تاثير الدقائق النانوية للفضة (Ag NPs) في بعض الجوانب التركيبية والوظيفية للجهاز التناسلي الذكري للفئران. جرعت الحيوانات فمويا بالـ Ag NPs بجرعة 5 ملغم/مل وللمدد 5, 10, 15 يوما وفي اليوم التالي لانتهاء كل مدة معاملة تمت التضحية | This study touched to know the effect of silver nanoparticles (Ag NPs) on some organs of the reproductive system and the testosterone hormone in male albino mice. animals were orally dosed with 200 mg/kg of Ag NPs for 5, 10, 15 days and the next day to the end of each period of treatment has animal sacrificed and autopsy after taking their weights before and after the treatment for the comparison between them and then took the testes and epididymis (head and tail) from them after measuring their weights. This study included the study of changes in the weight of animals and organs, sperm characters in both the testes and epididymis, the percentage of cells generating sperm, the number of Leydig cells and the diameters of these cell nuclei, changes in the seminiferous tubules in the testes and changes in epididymis tubule and histopathological changes as well as changes in testosterone level. The results of the animals weights before treatment showed a significant decrease (P<0.05) compared to the weights of the same animals after treatment, while the results of this study after comparing with the control group showed a significant decrease (P<0.05) in the weights of testes and tunica albuginea for the periods of 5, 10 and 15 days. The epididymis showed a significant decrease (P<0.05) in the average weights of the epididymis head for the three periods while the average weights of the epididymis tail showed a significant decrease (P<0.05) for the periods of 10 and 15 days. The vitality and the concentration of the sperms showed a significant decrease (P<0.05) in their percentages for the three periods. All the results above showed a significant decrease (P<0.05) when they compared between the three periods of the treatment. While the sperm abnormalities showed a significant increase (P<0.05) in their percentage in each of the testes and epidydimes for the periods 5, 10, 15 days with a significant increase (P<0.05) in these abnormalities when they compared between the three periods of the treatment. The histological results of this study after comparing to the control group revealed histopathological changes in the testes and the epididymis (head and tail). The percentage of spermatogenesis showed a significant decrease (P<0.05) in the number of spermatogonia, primary spermatocyte and sperms when compared to the control group with a significant decrease in the number of these cells when compared the three treatment periods with each other. While the number of secondary spermatocyte and spermatids was significantly increased (P<0.05) when compared with the control, with a significant increase in the number of these cells when comparing the three periods of treatment. Whereas the results of Leydig cells after comparing to the control group showed a significant decrease (P<0.05) in the number and the diameter of the cell nucleus. The results of this study also showed a significant decrease (P<0.05) in the thickness and the diameter of the seminiferous tubules of the testes and the thickness, The epithelium height of the epididymis (head and tail) when compared to the control group with significantly decrease in the thickness and diameter of the seminiferous tubules of the testes and the thickness and epithelium height of the epididymis (head and tail) when they compared between the three treatment periods. The results of this study when compared to the control group showed a significant decrease (P<0.05) in the level of the testosterone hormone with significantly increase (P<0.05) in the testosterone level when compared between the periods of 5, 10, 15 days. From these findings it is concluded that the AgNps crossed the blood - testis barrier and caused many changes to the testicular tissues and decreased the level of the testosterone. This may affect the fertility of mice.

تاثير تربية الاسماك بالاقفاص الموضوعة في نهر دجلة المار بمدينة بغداد في مجتمع الهائمات القشرية The Impact of Fish Farming By Cages Placed In The Tigris River At Baghdad City On The Community of Crustacean Zooplankton

اسم المؤلف: حارث قاسم مهدي
اسم المشرف: صباح فرج عبد الاحد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان الاثار البيئية لزراعة الاسماك في اقفاص التربية في العراق لم تتم دراستها بصورة جيدة،علما ان هذا النشاط يمارس على نحو متزايد وهناك حاجة كبيرة لدراسة الاثار المترتبة على ذلك.اجريت الدراسة الحالية على مواقع اقفاص تربية الاسماك في نهر دجلة شمال بغداد للتعر | The present study took place on fish farming cages located in the Tigris River in the north Baghdad, to in order to study the effects of high fish densities on zooplankton communies in addition to study of some physical and chemical factors.The environmental effects of fish farming in cages in Iraq have not been studied well, knowing that this activity is practiced increasingly and there was a significant need to study the implications of this important mater.The aim of this study was to investigate the effects of carpfish farming cages on the density of zooplankton in the Tigris River, The samples were taken twiece a month form the three stations for six months from January to June 2014 in three selected stations in the river stream : the first place was Al - Rashidiya station which located in Al - Rashidiya area (Kadhim Al - Ali), 5 km from tourist Baghdad island and the second station was Algriaat 1 which located in Algriaat area in Rusafa side of Baghdad city corresponding to the Kadhimiya near the bridge of riverain pedestrian (Al - Duab bridge) connecting between Al - Rashidiya, while the Al - Kadhimiya and the third station was Algriaat 2 which located in Algria’at zone corresponding to the area of Kadhimiya.The study included the identification studying the density of three grops of zooplankton which were Calanoida, Cyclopoida and Cladocera in addition to the measurement of some physical and chemical characteristics of river water and its effect on the three zooplankton community according to their importance, which included velocity of water current, water temperature electrical Conductivity and Salinity, pH, dissolved oxygen(DO), biological oxygen demand (BOD), phosphate concentration and nitrate concentration. The results of the present study showed that the density of Cladocera. Was lower than the densities of Calanoida & Cyclopida in the three station. on the other hand the current study showed that the densities of both Calanoida and Cyclopoida were oscillatory during the months of th study between highest and lowest, without in statistically significant differences, but the highest densities in June, and this study shows that the highest densities of the three groups of zooplankton were recorded in June. The present study showed a negative effect of the high density of the fish in the cages on the density rate of planktonic crustacea by reducing the numbers and the density of planktonic crustacea community inside cages. Compared with planktonic crustacea community outside the cages and with those within 100 meters far from cages, the differences were statisticaly singnficance.On the other hand regarding to the results of the physical and chemical factors it was found a highest that the velocity of water current rate was 82.50 cm/s in Algria’at 2, while the lowest was 43.33 cm/s in Al - Rashidiya station.Water temperatures ranged between 9C° in Algria’at 2 to 32C° in Al - Rashidiya station during the study period.The highest rate of electrical conductivity was 1032.00 ?S/cm in Algria’at 1, while the lowest was 765.25 ?S/cm in Al - Rashidiya station, while the highest rate of the salinity concentration in the fish cages was 0.66 mg/L in Algria’at 1 and the lowest concentration of salinity was in Al - Rashidiya 0.48 mg/L.pH values ranged between 7.20 in Algria’at 2 to 8.05 in Algria’at 1 in March for both stations, whil the concentration of dissolved Oxygen in water concentration ranged between 5.95 mg/l in Al - Rashidiya to 7.70 in Al - Rashidiya and Algriaat 1. and the Biological Oxygen Demand ranged was between 4.11 mg/l in Algriaat 1 to 6.50 mg/L in Al - Rashidiya station during February for both stations. It was also found that the lowest concentration of phosphate was 0.016 ml/l in Algriaat 2 in april while the highest concentration was 0.075 mg/l algreaat2 in march Also it was found that the lowest cocentration of nitrate 0.82 mg/l was recorded in al griaat 2 in fabruart while the highest conceneation 1.90 mg/l was recorded in Al Rashidya in june.All the results of the physical and chemical factors founded were within normal allowable environmentally boundaries and which have no effect on the aquatic planktonic crustacea community.

دراسة مجتمع الديدان الحلقية قليلة الاهلاب بنهر الفرات في المسيب - العراق Study of Aquatic Oligochaetes Community In The Euphrates River In Al - Mussayab / Iraq

اسم المؤلف: الهام عبيد صالح الجنابي
اسم المشرف: هيفاء جواد جوير
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعد ديدان قليلة الاهلاب المائية مجموعة حيوانية تضم انواعا مهمة من الناحية الاقتصادية والعلمية كونها غذاء جيدا للاسماك، ودورها في النظام البيئي بوصفها مؤشرات حياتية معروفة في تقييم نوعية المياه ودرجة تلوثها. وبسبب قلة الدراسات التشخيصية وعدم وجود قائمة خ | Aquatic oligochaete worms is an animal group that contains important species, economically speaking as its being a good fish food, and its scientific role in the ecosystem as well known bio - indicator for assessment water quality and evaluate the degree of water pollution. Due to little identification studies available about this group, and the absence of Iraqi checklist of species, the idea of the current study came, which concerned with the community of aquatic oligochaetes worms in the middle sector of the Euphrates River. Three sites have been chosen for this purpose, the first site is on the Euphrates River in the district of River at Al - Mussayab city (S1) and two streams branched from it, including Al - Mashroo'a stream (S2) and Abu - alasafeer stream (S3).These sites are characterized by sediment texture ranged from silt - sandy sediment in S1, sandy in S2 and Sandy - Clay in S3, the percentage of organic matter in the sediment ranged between1.8 - 2.6 % in S1, 0.7 - 1.9% in S2, and 4.8 - 6.4 % in S3. As far as plants concern, the presence of reed plant Phragmites australis, Potamgeton crispus plant, Hydrilla verticellata plant, and some filamentous algae in all study sites.Total of 1720 individuals of aquatic oligochaeta have been sorted out during the study period from all sites representing 37 species belong to the family Lumbricidae, Tubificoid naidid worms, Naidid worms of subfamily Naidinae and subfamily Pristininae, and finally, family Aeolosomatidae. Eleven species were considered as new records for Iraq. Regarding study sites, S2 recorded a least number of individuals and number of species, while the number of individuals and number of species were converged at S1 and S3. Results of the Species Richness Index showed that the highest value was recorded at the S3 and reached 8.37, while Species Uniformity Index, ranged between the highest value of 1.88 at the S3 and lowest value of 1.60 in S2, and finally in S1 it was 0.85. Shannon - Weiner diversity Index, recorded its highest value of 6.33 bits / individual in the S3, and lowest value has in of 2.78bits/individual in S1, but it was 4.80 bit / individual in S2. The Results of Jacquard Presence - Community values of similarity showed that the highest degree of similarity is (58.62%) recorded betwen S1 and S2. One species of family Lumbricidae was recorded that is Eiseniella tetraedra in S2.This family recorded a percentage of 3% of the total isolated worms. Total of 584 individuals of Tubificoid naidid worms were sorted with a percentage 34% of the total number of worms and they were identify as nine species included Limnodrilus hoffmeisteri, L. profundicola, L.claparedeanus, Tubifex tubifex, Aulodrilus pigueti, Peloescolex tenuis, Potamothrix hammoniensi, Rhyacodrilus coccineus, and finally Branchiura sowerbyi which recorded the highest percentage of 65.24% of the total tubificoid naidid worms with the highest frequency of 100%, followed by L. hoffmeisteri with a percentage of 16.95% and the frequency percentage of 85.19%, while the remaining species recorded close proportions ranged between 1 - 2%. Peloscolex tenuis was considered as a new record for Iraq, where10 individuals of this species were sorted with a percentage of 1.71%, and a frequency percentage of 3.70 %.Total of 47 individuals of subfamily Pristininae, were sorted, represented five species, including two species of genus Pristinella, P obsorni, and P. sima, and three species of genus Pristina, identified as, P. longiseta, P. aequiseta, and P. foreli, with a highest percentage of 53.19%, and a highest frequency of 14.81% were recorded by P. longiseta. A total of 788 individuals of subfamily Naidinae were sorted represent 20 specie includes Chaetogaster diastrophus, Stephensoniana trivandrana Paranais litoralis, P.frici, Ophidonais serpentina, Slavina appendiculata, S.isochaeta, Specaria josinae, Stylaria lacustris S. fossularis, Dero(Dero) evelinae, D.(D.) nivea, D. (Aulophorus) furcatus, Nais simplex, N.pseudobtusa, N. stolci, N. variabilis, N. elinguis, N. pardalis, Allonais inaequalis. Nine species of which were recorded for the first time in Iraq (in Bold). Species S. lacustris recorded the highest frequency percentage of 59.25%, and the highest individuals number 0f 187 was recorded by both S. lacustris and O. serpentine. Finally, 207 individuals of family Aeolosomatidae were identified into two species, Aeolosoma variegate and A. hemprichi. The first species recorded the highest percentage of 82.61% and a frequency percentage of 33.33%, and the second species recorded a percentage of 17.39% and a frequency percentage of 29.64%. The study also included a description of the identified species with photos illustrations of the identification characteristics of each species.

استخلاص وتوصيف المنشط السطحي الحيوي Rhamnolipid من بكتيريا Pseudomonas aeruginosa المعزولة من بعض الحالات السريرية والبيئية Extraction And Identification of Rhamnolipid Biosurfactant From Pesudomonas Aeruginosa Isolates From Clinical And Environmental Cases A Thesis Submitted

اسم المؤلف: مها هاني توفيق الخزرجي
اسم المشرف: ندى صباح رزوقي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدف البحث الى التعرف على فاعلية التدريس باستراتيجية التعلم بالتعاقد في تحصيل مادة علم الاحياء لدى طلاب الصف الثاني المتوسط وتفكيرهم الابداعي. وللتحقق من ذلك تم صياغة الفرضيتين الصفريتين الاتيتين : 1 - لا يوجد فرق ذو دلالة احصائية عند مستوى (05.0) بين مت | 50 isolates of the bacteria Pseudomonas aeruginosa was obtained from 201 clinical samples were distributed between Burn Specialist Hospital and teaching laboratories from patients with burns, infected wounds, middle ear infection, urinary tract infection and respiratory tract infection for a period from 1st February till 1st May. And we obtained 50 bacterial isolates from 20 samples from contaminated and non - contaminated soils were distributed different areas of Baghdad for a period of 1st May 2013 till 1st August.Number of cultural microscopically, biochemical and sensitivity to antibiotics tests had been, than diagnosis was confirmed by API20E system.These isolates was tested for ability to production of biosurfactants (rhamnolipid) by haemolysis, oil spreasding test, calculate value of emulisification factor (E24) and measuring surface tension for liquid media. Tow isolates (PS42 and PP8) had been selected, first one was from soil samples and other was from pathological samples because of they have highest productivity, haemolysis ability, oil spreading, highest emulisification factor value and highest in lowering surface tension, there for these tow isolates selected for study their inhibitory activity against types of bacteria. The rhamnolipid was extracted from tow isolates P. aeruginosa PP8 and P. aeruginosa PS42 by using mixture of solvents as was obtained 15.45 g and 18.25 g per liter of each of the PP8 and PS42 respectively. The rhamnolipid was diagnosed by thin layer chromatography technology (TLC) and high - performance liquid chromatography (HPLC) showed that tow bacteria produced three types of rhamnolipids (mono - , di - rhamnolipid and rhamnolipid A). The rhamonlipid efficiency was tested for inhibitory activity against bacteria by measuring diameter of inhibitory zone surrounding holes and discs. The inhibitory activity was high against Bacillus cersus bacteria followed by P. aeruginosa, than Staphylococcus aeraus and the lowest one was E. colli. The inhibitory activity for biosurfactant was approached to inhibitory activity for industrial surfactants. Also in this study has been determined minimum inhibitory concentration (MIC) value and Minimum Bactericidal Concentration (MBC) value for biosurfactants and the results varied depending on different types of biosurfactants and type of bacteria and the lowest values for MIC and MBC of biosurfactant produced by P. aeruginosa PS42 on growth of B. cereus and reached to 16.It was first time at local level for testing inhibitory activity of biosurfactant against pathogenic bacteria S. aureus and P. aeruginosa which was studied in vivo (injuried skin) after introduction these pathogens experimentally into laboratory mice with clinical symptoms appeared in injured skin after 48 hours and then treated mice groups with of 30 mg \ ml concentration of biosurfactants produced from isolates of bacterial (P. aeruginosa PP8 and P. aeruginosa PS42), resulted in a decrease in the time required for healing as found from the results obtained by the different length of period needed for healing (depending on the nature of injury and type of microorganism that causes injury).The mice had been recovered after 5 days when used biosurfactant at concentration 30 mg/ml produced P. aeruginosa PS42 followed by mice recovered after 6 days when used biosurfactants 30 mg/ml produced by P. aeruginosa PP8 in comparison with control group which recovered after 12 days for mice infected with S. aureus. In mice infected with P. aeruginosa recovered after 10 days when used biosurfactant produced by P. aeruginosa PS42 at concentration 30mg/ml and followed by mice recovered after 12 days when used biosurfactant produced by P. aeruginosa PP8 at concentration 30mg/ml in comparison with control group which recovered after 17 days.

دراسة وبائية لطفيليات القناة الهضمية عن منطقتي ابو غريب والعامرية وتاثيرها في بعض مستويات الدم Survey of Intestinal Parasites In Abu - Ghreeb And Al - Ameria Areas And Their Effect On Some Blood Components A Thesis Submitted

اسم المؤلف: دعاء بهاء عبد الجبار
اسم المشرف: طالب عبد الله حسين
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية اجراء مقارنة حول نسب انتشار الطفيليات المعوية وعلاقة الاصابة ببعض مكونات الدم المحيطي (اقيام الهيموغلوبين والعدد الكلي لكريات الدم البيض واعداد الحمضات) في مرضى من منطقتي ابو غريب والعامرية خلال المدة مابين شهر تشرين الاول 2011 ولغاي | The present study has included the comparison 0f prevalence rates of intestinal parasites and the relationship of infection of certain components peripheral blood (hemoglobin values, the total number of white blood cells, eosinophils numbers) in patients of each of the regions of Abu Ghraib and Amiriyah during the period from October, 2011 until July, 2012 as it has been collected (2449) stool samples that were distributed between (1430) stool samples from Abu Ghraib Hospital auditors and (1019) stool samples of auditors of the Health Center in Amiriya and also it has been collected blood samples. Stool samples were examined by direct method using brine (Normal Saline) and tincture of iodine (Lugol's Iodine) and the floating way by sulphate zinc water (ZnSo4.7H2O) to investigate the prevalence of intestinal parasites and the relationship of infection by age, sex, number of family members, and the type of water used for drinking. The results showed the presence of significant difference at the possibility(p<0.05) in total infection for the regions of Abu Ghraib and Ameriyah where the percentage was (36.29%) in the area of Abu Ghraib and (27.57%) for the Amiriyah area. It has been diagnosed five types of intestinal parasites (protozoa + Helminthes) as follows : A - Intestinal protozoa in the Abu Ghraib and for the Amiriyah area : 1 - Entamoeba histolytica was (17.62%, 16.68%).2 - Giardia lamblia was (11.95%, 5.49%).3 - Entamoeba coli colon was (2.44%, 2.55%(.B - Helminthes1 - Dwarf tapeworm Hymenolepis nana was (2.37%, 1.76.(2 - pinworm Enterobius vermicularis was (1.88%, 1.07.(We Did not observe any significant differences between the infection of intestinal parasites and sex factor where the total infection for males was (6.53%) while for females, it reached (8.06%) for Abu Ghraib, as to the Amiriya region, also we did not notice any significant differences between the infection of intestinal parasites and sex factor where The total infection for males was (7.26%) while for females, it was (6.84%(. It has been also found that there are significant differences and when probability of (P <0.05) for the infection with parasite (Giardia lamblia) between the areas of Abu Ghraib and Amiriyah where infection rate reached (11.9%) in Abu Ghraib while for the Amiriyah area, it has reached the ratio of, (5.49%) for the same parasite, while there was no significant difference between the other four types of parasites. The individual injuries are prevalent Lama has reached bilateral injuries (56) bilateral injury in the Abu Ghraib area, and (13) in the area of bilateral injury Ameria and most of the injuries were bilateral parasite (E.histolytica+ G.lamblia). The results showed that there is a significant difference when probability (P <0.01) for the distribution of age groups, reaching the highest infection rate in the age group of (29 - 20) years, (56.68%) and the lowest infection in the age group (60 years and over) the percentage of infection was (21.42%) in the Abu Ghraib area, while for Amiriyah area, it has been recorded the highest infection rate in the age group of (29.20), where it reached (49.22%) and the lowest injury in the age group (60 years and above) where The percentage of infection was (4.82%(. Also, it was noticed the emergence of variation in the values of the components of the peripheral blood and the lack of significant difference when probability (P <0.05) in the area of Abu Ghraib where it recorded the highest proportion of hemoglobin in the parasite (Entamoeba histolytica), amounting to (12.40 g / 100 ml) and the lowest percentage hemoglobin was recorded in parasite (Enterobius vermicularis) amounting to (9.46 cells / mm3), and the lack of a significant difference in the total number of white blood cells where it recorded the highest rate in the parasite (Entamoeba histolytica) 6776.32 cells / mm3 while the lowest rate was recorded in the parasite (Enterobius vermicularis (5074.37cell\mm3, and lack of a significant difference for eosinophile numbers was recorded the highest proportion in parasite Hymenolepis nana 516.63 cell\mm3 and the lowst rate was recorded in parasite Entamoeba histolytica 159.63 cell\mm3. As for the Amiriyah, it hasn’t been noticed any significant differences when probability (P <0.05), where the highest proportion of hemoglobin in the parasite (Entamoeba histolytica), was (11.76 g / 100 ml) and the lowest percentage hemoglobin recorded in parasite (Enterobius vermicularis) was (9.90 g / 100ml), and there was the lack of a significant difference in the total number of white blood cells, where it has been recorded the highest rate in the parasite (Entamoeba histolytica) 6574.59 cells / mm3 and the lowest rate was recorded in the parasite (Entamoeba coli) 5700.00 cells / mm3 and the lack of a significant difference for eosinophile numbers. The highest proportion in parasite Enterobius vermicularis was 518.65 cell\mm3 and the lowst rate in parasite Entamoeba coli was 140.29 cell\mm3. The results also showed that there is a high significant difference when the probability (P <0.01) in the incidence of intestinal parasites and the relationship of water used for drinking where the study proved that the highest infection rates recorded of the people who use canal waters and water tank where It percentages was (49.54%, 39.00%), respectively while the lowest rate of infection was recorded in people who use boiling water which reached (14.02%). The same applies to the Amiriya district which recorded the highest percentage of people who use tap water and the lowest percentage of those using boiling water reaching ratios (41.49 %, 12.5%), respectively.

دراسة تشخيصية لانواع تحت العائلة Tubificinae (قليلة الاهلاب : نايديدي) من بيئات مائية مختلفة داخل مدينة بغداد - العراق An Identification Study On Subfamily Tubificinae (Oligochaeta : Naididae) From Different Aquatic Habitat In Baghdad / Iraq

اسم المؤلف: افراح محمد علوان الجبوري
اسم المشرف: هيفاء جواد جوير
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: انواع تحت العائلة Tubificinae ديدان مائية حمراء اللون يتراوح طولها مابين 1سم الى عدة سنتمترات وقطرها حوالي 1ملم تنتمي الى العائلة Naidiadae، الصنف الثانوي قليلة الاهلاب، صنف السرجيات التابع لشعبة الديدان الحلقية، وتسمى عاميا" بديدان الوحل. تقطن هذه المجم | Species of Subfamily Tubificinae are aquatic red worms, their length ranged between 1cm to several centimeters, and their diameter about I mm, and they are classified with Family Naididae, subclass Oligochaeta, class Clitellata, phylum Annelida. They are commonly called "sludge worms". This group inhabit the rivers, lakes and ponds sediment, and some species also found in marine habitat, they are considered as one of the main benthos components specially in freshwater, with increase abundance in soft sediment rich in organic matters. Sludge worms individuals are hermaphrodite, reproduced sexually by cross - fertilization, eggs led in cocoon and hatched directly without any larval stage. They are also reproducing asexually by ArchitomyAccording to the economic importance of this worms as good food for fish, their ecological role as well known bio - indicators, little studies about their identification were available and absence of check list of their species in Iraq, the present investigation idea was come to concern primarily with sorting and identification of subfamily Tubificinae from River Tigris and some water surface in Baghdad /Iraq. For this purpose six study sites were chosen, including a drainage canal, North Baghdad (S1); three sites on the shore of River Tigris (S2, S3 & S4) ; in addition to site in Al - Jeish canal, East Baghdad (S5), finally the sixth site was from pond in Al - Zawra'a park, within the center of Baghdad (S6). These sites were characterized by sedimenet rang from clay, silty - clay to silty with a percentage of organic matter ranged between higher percentage of 9.9% in site S5 and 0.7% as a lowest percentage in site S1. According to water temperature, salinity and pH values, they were identical in all study sites.3628 individuals were sorted from 24 samples collected from all study sites. The sorting results revealed that the highest number of worms (1346 ind.) recorded in site S5, while the lowest number (326ind.) recorded in site S4. Twelve species were identified belonging to seven genera, and Limnodrilus was the most dominant genus. Five species were considered as new records to Iraq, which are L. silvani in S6, P. hammoniensis & Monopylophorus irroratus in P. moravicus in S2 and Rhyacodrilus cocciensis in S6.Five species of Limnodrilus were recorded, including L. hoffmeisteri, L. claparadienus, L. profundicola, L. udekemianus, and L. silvani with a percentage of 52%, 1.40%, 4%, 10.03% and 0.35% for each species respectively. Two species of Potamothrix were recorded which are P. bavaricus and P. hammoniensis with a percentage of 2% and 2.05% respectively, in addition to Branchiura sowerbyi, Psammoryctides moravicus, Tubifex tubifes, Rhyacodrilus cocciensis, and Monopylophorus irroratus with a percentage of 26.07%, 0.33%, 3%, 0.27, and 0.24 respectively. The higher frequency percentage of 100% was recorded by L. hoffmeisteri and B. sowerbyi.High densities of Tubificinae were recorded during all study period. Higher density of 6882 ind./m2 was recorded during in site S1; 4296 ind/m2 in site S2 ; 4496 ind/ m2, ind/ m2in site S3; 3730 ind/ m2 in site S4; 15218 ind/ m2 in site S5 and 7026 ind/ m2 in site S6.The results of relative abundance revealed that L. hoffmeisteri was the dominant species in site S3 and abundant in other study sites. B. sowerbyi was abundant in all study sites, while other species were recorded between few to rare species except L. udekemianus which was abundant in site S5, and T. tubifex in site S4. It was clear from species richness index, Shannon - Wiener diversity index, and species uniformity index, that the highest value for each index was recorded during cold months (Dec. - Feb.), 1.47, 2.83bit/ind, and 1.17 respectively, while the result of cluster analysis depending on Jaccard index of similarity revealed that the highest similarity of 80% found between S1& S6.The investigation also includes description of identified species and photos of identification criteria for each species. B. sowerbyi was easily recognized from other species by having gill filaments at the posterior end of the body. Species of Limnodrilus were characterized by the absence of hair chaetae, and they are differentiated by the shape of penis sheath, due to the close similarity of chaetal shapes, with the exception of L. udekemianus in which its anterior chaetae characterized by long and curved dorsal tooth. Species of Potamothrix were recognized by the presence of a pair of spermathecal chaetae, its shape was used to differentiate between P. hammoniensis, which have long gutter - shape and P. bavaricus, which have plade - like shape with triangle base. P. moravicus also have spermathecal chaetae but it was differed from that of Pomatothrix species by its long and narrow shape. T. tubifex was identified by its tub - shaped penis sheath, while R. cocciensis, was recognized by their penial chaetae in segment NO. XI, and M. irroratus by the presence of pseudopenis and its sheath.

دراسة بعض الجوانب المناعية لاصابات اللشمانيا في عينة المرضى العراقيين Study of Some Immunological Aspects of Leishmaniasis In Sample of Iraqi Patient

اسم المؤلف: مرتضى قيس علي العامري
اسم المشرف: انوار ادريس سليمان العساف
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study was carried on 145 patients infected with leishmaniasis including; 75 with CL, 70 with VL, attending Al - Karama Teaching Hospital, Central Public Health Laboratory and Central Pediatric Teaching Hospital, during the period October 2013 to February 2014 were enrolled in this study, in addition to 30 healthy control collected from the National Center for blood donation for the same age groups for a group of patients. No clinical autoimmune manifestations such as arthralgia or cutaneous vasculitis were reported in any patients. Based on direct skin smear examination and serum samples analysis, the patients were distributed into two clinical groups : 30 patients suffering from cutaneous leishmaniasis and 30 patients suffering from visceral leishmaniasis patients.The presented study aimed to confirmation of diagnosis cutaneous leishmaniasis depending on direct skin smear and visceral leishmaniasis based on indirect immunofluorescent assay test IFAT, then investigate the association of IgG autoantibodies against some antigens, especially : nuclear antigens, like : ds - DNA, nucleosome, histones, SmD1, PCNA, ribosomal P0, SS - A/Ro60kD, SS - A/Ro52kD, SS - B/La, CENP - B, Scl70, U1 - snRNP, AMA M2, Jo1, PM - Scl, Mi - 2, Ku and against cardiolipin, in order to correlate the types of diseases presentation (cutaneous or visceral) with the presence of these autoantibodies in a sample of Iraqi patients.The study was reached to the following results : 1. The direct microscopically examination of skin smear in CL patients, showed positive results in 30 cases (40.0%) compared to 45 cases (60.0%) negative results, and was a significant difference at the level of (P?0.05).2. The anti - leishmanial antibodies by Indirect immunofluorescent assay test in VL patients, showed positive results in 30 cases (42.9%) compared to 45 cases (57.1%) negative results, and was a highly significant difference at the level of (P? 0.01).3. The age groups most affected in CL were 16 to 40 years, with high significant differences at the level of (P? 0.01) were observed when compared with the other age groups. As for patients with VL, who aged between 3 months to 9 years, they showed variable significant differences between age groups in female while there were no significant differences in male at the level of (P?0.01). The majority of patients were aged 3 to 4 years and they represent 9 (30.0%). The study result showed 16 CL patients (53.3%) have only one lesion, while 11 patients (36.7%) have 2 to 9 lesions and only 3 cases (10%) have ? 10 lesions in different sites of their bodies. There were elevated high significant differences at the level of (P?0.01) when compared between the groups. And 21 cases (70%) have infected for ? two months, and the remaining 9 cases get their infection for > 2 months duration. There were increased high significant differences observed at the level of (P?0.01) when compared between the groups. Dry lesions recorded superiority in 20 (66.7%) of the CL cases, and other 10 (33.3%) have been diagnosed as a wet lesions. There were elevated high significant differences observed at the level of (P?0.01) when compared between the two types of the lesion. And The upper limbs were the dominant site of lesions in the CL cases, 15 (39.4%) of the cases had their lesions in one or both hands, followed by the lower limbs in 11 (29.0%) cases and the less frequent site of infection was the face in only 4 (10.5%) cases and 8 (21.0%) cases common lesions. The results showed obvious high significant differences at (P? 0.01) between the groups.4. For the tests of the autoantibodies, the anti - ds - DNA antibody was positive only in one patients (3.3%) with CL of the total (30) patients, while tests of the rest 29 (96.7%) were negative, while in VL of the total (30) patients, 9 patients (30.3%) were positive and 21 (70.0%) were negative. The anti - ds - DNA in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the study groups. As for the anti - nucleosomes antibodies, it was positive in 5 patients (16.7%) and negative in 25 (83.3%) among patients in CL, while in VL patients, 16 patients (% 53.3) registered positive and 14 (46.7%) their result werenegative, the anti - nucleosomes antibodies in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the groups study. No score was recorded for anti - SmD1antibodies in CL patients, no one was positive 0 (0.0%) and all the 30 patients (100.0%) gave negative result for this antibody, while in patients with VL, the result showed 4 patients (13.3%) gave positive and 26 patients (86.7%) were negative, the anti - SmD1antibodies in leishmaniasis patients was significantly different at the level of (P?0.05) when compared between the groups. While the anti - SSA/Ro60kD and anti - SSA/Ro52kD, in VL patients, 9 patients (30.0%) scored positive results and 21 of the patients (70.0%) were tested negative for anti - SS - A/Ro60kD, but 7 patients (23.3%) were positive and 23 of the patients (76.7%) were tested negative for anti - SS - A/Ro52kD. While in patients with CL, no one scored positive (0.00%) and 30 of the patients (100.0%) were negative for anti - SS - A/Ro60kD, whereas one patient (3.3%) gave a positive result and 29 of the patients (96.7%) were negative for anti - SS - A/Ro52kD. About anti - SS - B/La we found that 19 of the patients (63.3%) were scored positive result for this antibody in addition to 11 patients (36.7%) who were negative in VL patients and 4 of the patients (13.3%) have those antibodies and 26 of the patients (86.7%) haven’t these antibodies in CL patients respectively. The anti - SS - A/Ro60kD, anti - SS - A/Ro52kD and anti - SS - B/La in leishmaniasis patients were highly significantly different at the level of (P?0.01), when compared between studied groups.5. VL cases showed a significantly different distribution of anti - nuclear antibodies (ANA) in compare with CL cases, especially; in Anti - Ku antibodies 24 (80%), anti - Mi - 2 antibodies 21 (70%) and anti - SS - B/La antibodies 19 (63.3%) respectively.6. The results of anti - nuclear antibodies level in patients with leishmaniasis like; anti - ds DNA, anti - nucleosome, anti - SmD1, anti - PCNA, anti - SS - A/RO 60, anti - SS - A/RO 52, anti - CNP - B were in high significantly different at the level of (P?0.01) and level (P?0.05) when compared between the groups, while the other autoantibodies like; anti - histone, anti - ribosomal P0, anti - Sc170, anti - U1 - snRNP, anti - AMA M2, anti - J0 - 1 and anti - PM - Sc1 showed no significant differences (P?0.01) between the investigated groups.7. The mean value of anti - cardiolipin IgG antibodies was increased substantially with highly significantly differences (P?0.01) in VL patients as compared with control group (8.123 vs. 1.959), also as compared with CL patients (8.123 vs. 2.402).

تقويم صفة تحمل بعض اصناف البطاطا المكثرة نسيجيا للجفاف على المستوى الجزيئي Evaluation of Some Potato Varieties Grown In Vitro To Drought Tolerance At The Molecular Level

اسم المؤلف: هوازن حليم صالح
اسم المشرف: علي عبد الامير مهدي الصالحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: درست استجابة اربعة اصناف من البطاطا Solanum tuberosum L. وهي (Lusa, Ambo, Arizona, Riviera)، استجابتها للزراعة النسيجية، وتقييم صفة تحمل الجفاف، كما اجريت التحاليل الجزيئية لتحديد البصمة الوراثية لها.اوضحت نتائج تجربة اختبار استجابة الزراعة النسيجية، ان ا | Four varieties of Potato (Solanum tuberosum L. Lusa, Ambo, Arizona and Riviera) were studied for their response to tissue culture and evaluation of the treatment of drought tolerance and molecular analysis. Results showed that the varieties differ in their response to growth, where as Arizona was the best variety in shoot tip culture and the survival percent (100%) and gave the highest average of branch length and number of leaves which were 9.810 cm, 8.100 leaf/ plant, while Riviera showed lower rate of survival and growth (40%). Riviera was excluded from the experiments. Cytokines and auxins were used in the following concentrations Kinetin (kin) with 2, 4 mg/L and Benzel adenine (BA) with interacted with Indoleacetic acid (IAA) at 1 or 2 mg/l or Napthalene acetic acid (NAA) at 0.5 or 1.0 mg/L in multiplication experiment. Arizona variety exhibited the highest length shoot, number of nods, number of leaves, root length, dry weight of roots, fresh and dry weights of shoots when using kinetin 2mg/L; interacted with NAA at 0.5 mg/L. while it showed a significant increase in the average of branches number, stem height, fresh and dry weights of shoot as well as the number of roots after treatment with using BA at 1 mg/L in combination with 0.5 mg/L (IAA). Drought tolerance of the studied varieties was studied by using Poly Ethlene Glycol (PEG) at the concentrations 20, 40, 60, 70, or 80 g/l. It showed that proline content was at its highest in Ambo variety reached 22.811 ? /g at the concentration 80 gm/L PEG. Arizona gave the highest mean in number of branches, number of nods, number of leaves, number of roots, under water stress conditions when the medium was supplemental with 20 mg/L PEG. Molecular analysis the studied varieties was conducted by using 6 primers. Results showed that the inheritance approximation between them, 0.314, 0.297, 0.273 for Lusa, Ambo and Arizona respectively. These results indicate that Rivera variety is genetically different from the others.

الكشف الجزيئي عن بكتريا Oxalobacter formigenes باستخدام جينات 16SrRNA، oxc في البراز من اشخاص اصحاء Molecular Identification of Oxalobacter Formigenes Bacteria Based On 16S rRNA, Oxc Genes In Stool Samples Healthy Subjects

اسم المؤلف: طارق طلال خلف
اسم المشرف: زهرة محمود الخفاجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: بكتريا Oxalobacter formigenes من مكونات المكنون البشري الميكروبي، تتغذى على الاوكزالات السامة وبذا تخلص الجسم منها. البكتريا سالبة لصبغة كرام، ولا تكون الابواغ ولاهوائية المعيشة، ويمكن عزلها من الغائط البشري، ولدراسة البكتريا من النواحي الجزيئية، تم جمع ع | Oxalobacter formigenes is one of the human microbiome, uses the toxic oxalate for their growth (Oxalotroph), and helps the body to get rid of excess oxalate. The bacterium is Gram negative, non - spore former, obligate anaerobe, and can be isolated from human stool.To carry out molecular studies on this bacterium, 50 samples of human stool were collected from subjects with wide range of ages (3 - 63 years) and different genders, subjects were healthy and have no medications and especially antibiotics for at least for 3 months. Genomic DNA was extracted with special kit, to amplify certain housekeeping genes, these are 16S rRNA (3' end) segment having the anti - Shine - Dalgarno sequence (ASD), and the oxc gene responsible for production of oxalyl - CoA decarboxylase (middle region responsible for active site at where the Thiamin pyrophosphate binds, the enzyme co - factor). It was possible to amplify the 16S rRNA gene in 46 sample out of 50 (92%), and 7 samples out of 50 (14%) for oxc gene. Analysis and alignments of 16S rRNA sequences put the Iraqi samples in the O. formigenes i.e., the same species depending on Distance Score of alignments, it has been found that ASD of bacteria is conserved and represented by the sequence " 5' CGCGGUGAACGUUCCCGGG3' " in the studied samples and in strains found in the International Databases. Segment of oxc (~ 600 bp) translated into proteins (190 - 194 amino acids) were aligned with oxc protein of O. formigenes (M77128) reference strain, it has been found that this segment similar to the TPP binding site and characterized by its high hydrophobicity

تاثير المستخلص المائي الخام لنبات الزنجبيل Roscoe officinale Zingiber على بعض الجوانب الدموية والتناسلية في الفئران الحوامل Effect of Crude Aqueous Extract of Zingiber Officinale Roscoe On Some Hematological And Reproductive Aspects In Pregnant Mice

اسم المؤلف: رغد خالد موفق مسلم
اسم المشرف: محمد عبد الهادي غالي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للتعرف على مدى تاثير المستخلص المائي الخام للزنجبيل Zingiber officinale Roscoe (الطازج، اوالجاف) على الفئران الحوامل المجرعة عن طريق الفم وبالتراكيز (284، 568، 1136، 1420 ملغم/ كغم) اثناء مراحل الحمل المختلفة (المبكرة والوسطية والمتاخرة | This study was designed to investigate the effect of the crude aqueous extract of ginger (Zingiber officinale Roscoe) (fresh or dry) on pregnant mice orally administered at doses (284, 568, 1136, 1420 mg/kg) during different durations of pregnancy (early, intermediate, and final) for a period of 7 days for each duration. The study showed that there was no significant difference in the average weights of the animals at (P < 0.05) after treatment with (dry or fresh) extract at doses (284, 568, 1136 or 1420 mg/kg) comparing with the control group.The parameters of blood in the early duration of pregnancy showed a significant decrease in the average of hemoglobin (Hb) at (P < 0.05) after treatment with (fresh or dry) extract at the doses (568, 1136, 1420 mg/ kg), while the average of packed cell volume (PCV), demonstrated a significant decrease after treatment with fresh extract at doses (284, 568, 1136, 1420 mg/kg), and at (568, 1136, 1420 mg/kg) after treatment with dry extract at (P < 0.05) : The average of red blood cells (R.B.C.) after treatment with (fresh or dry) ginger extract, at (P < 0.05) showed a significant decrease at doses (284, 568, 1136, 1420 mg / kg), while the average mean corpuscular hemoglobin (MCH), and the average mean corpuscular volume (MCV) at (P < 0.05) revealed a significant increase and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract, at doses (1136, 1420 mg/kg) for the average of (MCH), at doses (568, 1136, 1420 mg/kg) for the average (MCV) after treatment with fresh extract, while the average Mean corpuscular hemoglobin concentration (MCHC) did not show any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the intermediate duration of pregnancy showed a significant decrease at (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, while dry extract demonstrated a significant decrease in average of (Hb) and (PCV) at doses (568, 1136, 1420 mg/kg), and a significant decrease in the average of (R.B.C.) but at doses (284, 568, 1136, 1420 mg/kg), while the average of (MCH) and (MCV) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while the average of (MCHC) did not showed any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the final duration of pregnancy revealed a significant decrease (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while (MCH) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, (MCHC) did not demonstrated any significant difference at the level (P < 0.05) after treatment with (fresh or dry) extract at all doses, while (MCV) showed a significant increase at (P < 0.05) and at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract comparing with the control group. The results showed a significant decrease at (P < 0.05) in the average of diameters of ovarian and corpus luteum after treatment with (fresh or dry) extracts in the early and final durations of pregnancy at doses (568, 1136, 1420 mg/kg), and in the intermediate duration after treatment with (fresh or dry) extract but at doses (284, 568, 1136, 1420 mg/kg) comparing with group control.As for the concentration of the hormone estrogen (? - estradiol) didn’t demonstrated a significant difference at (P < 0.05) during different durations of pregnancy after treatment with (fresh or dry) extract at all doses, whereas progesterone showed a significant decreased at doses (284, 568, 1136, 1420 mg/kg) in all durations after treatment with (fresh or dry) extract comparing with the control group. Using doses at (284, 568, 1136, 1420 mg/kg) with (fresh or dry) extracts revealed a significant increase at (P < 0.05) in the average number of infected follicles in the all durations of pregnancy comparing with the control group. The results showed that there are no significant difference in the lengths of the embryos during intermediate and final durations at (P < 0.05) after treatment with (fresh or dry) extract at all doses comparing with the control group.There are also histopathological changes in the ovary, uterus and placenta included degeneration in ovarian follicles, necrosis, congestion, hemorrhage, edema, vasodilatation and rupture in blood vessels at different using doses.These findings indicated that the treatment with (dry and fresh) extract at doses (568, 1136 or 1420) mg/ kg harmful on pregnancy in different durations (early, intermediate, and final) , also the using of fresh ginger more harmful than dry ginger.
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