التحري عن بعـض عوامل الفوعة لبكتريا المكورات Enterococci المعوية المقاومة للمضاد الحيوي الفانكومايسين == Detection of Some Virulence Factors of Vancomycin - Resistant Enterococci
Author name:
حيدر صباح كاظم الخماسي
Supervisor name:
مي طالب فليح
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Baghdad
Language:
English
University location:
Baghdad
First pages:
24T2859 - p.pdf
Abstract:
تم الحصول على (20) عزله لبكتريا المكورات المعوية البرازية من اصل (135) عينة جمعت من مستشفى مدينة الطب ومستشفى الكندي وهي كما ياتي (15 عينة الادرار, 60 عينة دم, 50 عينة من قنوات الجذر للاسنان و10 عينة من الحروق).اختبرت الحساسية لهذه العزلات العشرون لـ 11 | Clinical sampling was carried out between September and December 2013, Twenty Enterococcus faecalis isolates were obtained from 135 clinical specimens. The samples included of patients in Medical City Hospital and Al - kindy Hospital (15 urine, 60 blood, 50 root canal and 10 wound swabs) Antibiotics susceptibility test for 20 isolates was done against 11 antibiotics, it was revealed that the isolates showed multi drug resistance were (18) isolates. The vancomycin susceptibility was determined by the minimum inhibitory concentration (MIC). Resistant and intermediate resistant to vancomycin was distributed among isolates at a ratio of 65% and 20%, respectively. Imipenem was found to be the most bactericidal agent against E. faecalis isolates E. faecalis virulence factors were detected phenotypically, The results showed that all isolates (100%) were hemolysin, protease and aggregation substance producer. 30% of isolates showed an ability to produce gelatinase. While (40.7%) of the isolates were a lipase producer. The results of the tube method showed that all E. faecalis isolates (100%) were slime layer and biofilm producer but the amount of adherent layer were different among the isolates ranged from strong to moderate and weak.The extracted DNA was subjected to Polymerase chain reaction (PCR) technique in a monoplex pattern to amplify the virulence factor Enterococcal Surface Protein (esp) which is chromosomal, results of this investigation showed that 20 (100%) E. faecalis isolates gave the amplicon size 933 base pair for the esp gene.The genetic determinants of Vancomycin - Resistant vanA and vanB genes were amplified using monoplex and multiplex PCR techniques in order to identify vancomycin resistant (van+) and sensitive (lacking van) among (13) E. faecalis. The vanA, vanB genes were detected in 11 and 4 E. faecalis isolates, respectively. The results of monoplex and multiplex PCR revealed that the molecular weight of vanA and vanB genes were 550 and nearly 600 bp, respectively. The results revealed that the vanA and vanB amplicons have a genetic variation in their molecular weight during the electrophoresis of PCR product.
