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التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني == Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients
Author name:
احمد كاظم محمد
Supervisor name:
محمد ابراهيم نادر | بتول حسن الغرابي
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
University of Baghdad - Institute Of Genetic Engineering And Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
First pages:
24T2977 - p.pdf
Abstract:
داء السكري مرض واسع الانتشارعالميا تختلف نسبة الاصابة بين البلدان المتطورة والنامية ويعتبر المسبب الرئيسي للاعاقة والموت في العالم.داء السكري النوع الثاني هو الاكثر حدوثا حيث تبلغ نسبة الاصابة (90 - 95%) من مجموع المصابين بالسكري للانواع الثلاثة الرئيسية. | Sixty of non insulin dependent diabetes mellitus (NIDDM) patients who were diagnosed according to American Diabetes Association criteria (ADA) 2007 were selected from the specialized center of endocrinology and diabetes (Baghdad AL - Russafa Health Directorate) during the period between first of May 2013 to last of October 2013.Their age ranged 35 - 70 years. Fourty apparently healthy individuals their age ranged (35 - 70) years were considered as control. Enzymatic colorimetric methods used for measured FBS (fasting blood sugar) and HbA1c (glycohemoglobin) and enzyme linked immunosorbent assay (ELISA) for hormones and enzyme markers. Fasting blood sugar revealed high significant in patients with median (11.6 mmol/L vs. 5.9 mmol/L) and (P<0.001) in comparison to control groups.Elevation of glycated hemoglobin (HbA1c) with mean (9% vs. 5.5%) in comparison to control groups. Another reliable marker are the concentration Adpionectine hormone, Insulin hormone and adenosine deaminase activity the results of those estimated significantly difference between levels mean (20.7 vs. 34 ng /ml) in patients compared to healthy subjects (P<0.001) for adiponectin ; mean (106.6 vs. 59.3 ng/ml) for ADA (adenosine deaminase) with (P<0.001) and the median (12.1 vs. 16 uIU/ml) for insulin hormone with (P 0.001). In order to investigate the accuracy of previously mentioned tests, a statistical analysis [Receiver - Operator Characteristics (ROC)] has been applied to show the accuracy, specificity and sensitivity of the tests under test.This analysis revealed that serum ADA activity is the best marker with highly specificity 100%, sensitivity 100%, and accuracy 100% while; FBS was the best test with highly specificity 100%, sensitivity 100% and 100% accuracy in comparison with other tests. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction - Sequences Specific Olegionucleotide (PCR - SSO) technique. The present study revealed that diabetic patients were positively associated with HLA - DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison with healthy control groups.This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2(Homeostatic Model Assessment2) ? - function, HOMA2 - IR, serum adiponectin, serum ADA and HLA alleles(DQB1*0201, DRB1*1137, DRB1*0401, DQB1*0501, DRB1*1306) in spite the significant associated between FBS and serum ADA and HLA - DRB1*0701 allele with (P 0.021, P 0.008) respectively.The current result concluded that there may be an important role for HLA genotyping in arising the chance for enhancing the susceptibility for either disease development or protection against its initiation.
