دراسة نسجية وكيمياء نسجية للقناة الهضمية في طائر فاختة النخيل Streptopelia senegalensis == Histological And Histochemical Study of The Alimentary Canal In Laughing Dove Streptopelia Senegalensis
Author name:
خولة خلف حسون صادق
Supervisor name:
مختار خميس محمد سعيد
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Baghdad
First pages:
24T2841 - p.pdf
Abstract:
تمثل الدراسة الحالية دراسة التركيب المظهري والنسجي وكيميائية النسيج للقناة الهضمية للحمام البري فاختة النخيل Streptopelia senegalensis, لثمان عينات من النماذج الحية البالغة من الحمام البري التي تم اصطيادها حية باطوال مختلفة, وبعد تخديرها بمادة الكلورفورم, | The present study represent the Anatomical, Histological and Histochemical structures of the alimentary canal in the laughing dove Streptopelia senegalensis, for eight adult specimens of these wild birds were trapped alive in different lengths, The specimens were anaesthetized by chloroform, and then carefully dissected for studying the gross anatomy, the alimentary canal from the proximal esophagus to the cloaca was carefully removed from the body cavity, lengths have been taken for alimentary canal and for each part of it, the alimentary canal divided into small pieces for each part, then fixated with Bouin's solution for general histological studies.Transverse sections of the different studied samples were cut at thickness of 5 - 7microns and the prepared sections were stained with routine stains Haematoxylin and Eosina and special stains for histochemical studies ( (Periodic acid - Schiff (PAS) technique, Alcian blue - PAS method and the Mercury bromophenol blue) ). The present Anatomical study showed the esophagus as long tubular organ, the cervical part of it is longer than the thoracic part and the crop was merely enlargement sac in shape which locate at the entrance of the thorax. The stomach is differentiated into a glandular proventriculus and a muscular ventriculus or gizzard.The small intestine distinguish into duodenum, jejunum and ileum which is the longest part of it, whereas the caeca are very small pair, and the rectum is short tube which end with cloaca, the last part of the alimentary tract. The histological study revealed that the alimentary tract composed of usual four layers; tunica mucosa, tunica sub mucosa, tunica muscular and tunica adventitia or serosa. The mucosal layer of esophagus was arranged in folds which was longer in the cervical part and constituted by nonkertenized stratified squamous epithelium, esophageal glands found just in submucosal tunica of the thoracic part. The crop has the same histological structure as in esophagus but it’s folds are less high than them in esophagus and there is no glands on it. The superior gastric glands of proventriculus were simple tubular glands and the deep gastric glands were compound alveoli glands which involve the submucosa tunica.A thick layer of koilen covers the mucosa of the ventriculus it’s lamina propria had loose connective tissue with simple alveolar glands. The villi of small intestine become shorter toward the rectum, which lining by simple columnar epithelium with striated border, and contain goblet cells which gradually increase in number frequently from the duodenum to the rectum. The histochemical studies revealed that PAS and alcian blue / PAS stains were given positive react in the esophageal glands, mucosa layer of esophagus, crop, stomach, and superior gastric glands and the goblet cells of small intestine and rectum. whereas the alcian blue / PAS were givin weak react in deep gastric glands in the provetriculus and gastric gland of gizzard but goblet cells were givin positive react in small intestine and rectum.Proteins were observed in the mucosa layer of esophagus, glandular stomach, intestine and rectum, when they had taken positive react with Mercuric bromophenol blue stain, but the reaction appeared weak in esophagea glands, intestinal glands and goblet cells.
