استخلاص وتوصيف المنشط السطحي الحيوي Rhamnolipid من بكتيريا Pseudomonas aeruginosa المعزولة من بعض الحالات السريرية والبيئية == Extraction And Identification of Rhamnolipid Biosurfactant From Pesudomonas Aeruginosa Isolates From Clinical And Environmental Cases A Thesis Submitted
Author name:
مها هاني توفيق الخزرجي
Supervisor name:
ندى صباح رزوقي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
Arabic
University location:
Baghdad
First pages:
24T2813 - p.pdf
Abstract:
هدف البحث الى التعرف على فاعلية التدريس باستراتيجية التعلم بالتعاقد في تحصيل مادة علم الاحياء لدى طلاب الصف الثاني المتوسط وتفكيرهم الابداعي. وللتحقق من ذلك تم صياغة الفرضيتين الصفريتين الاتيتين : 1 - لا يوجد فرق ذو دلالة احصائية عند مستوى (05.0) بين مت | 50 isolates of the bacteria Pseudomonas aeruginosa was obtained from 201 clinical samples were distributed between Burn Specialist Hospital and teaching laboratories from patients with burns, infected wounds, middle ear infection, urinary tract infection and respiratory tract infection for a period from 1st February till 1st May. And we obtained 50 bacterial isolates from 20 samples from contaminated and non - contaminated soils were distributed different areas of Baghdad for a period of 1st May 2013 till 1st August.Number of cultural microscopically, biochemical and sensitivity to antibiotics tests had been, than diagnosis was confirmed by API20E system.These isolates was tested for ability to production of biosurfactants (rhamnolipid) by haemolysis, oil spreasding test, calculate value of emulisification factor (E24) and measuring surface tension for liquid media. Tow isolates (PS42 and PP8) had been selected, first one was from soil samples and other was from pathological samples because of they have highest productivity, haemolysis ability, oil spreading, highest emulisification factor value and highest in lowering surface tension, there for these tow isolates selected for study their inhibitory activity against types of bacteria. The rhamnolipid was extracted from tow isolates P. aeruginosa PP8 and P. aeruginosa PS42 by using mixture of solvents as was obtained 15.45 g and 18.25 g per liter of each of the PP8 and PS42 respectively. The rhamnolipid was diagnosed by thin layer chromatography technology (TLC) and high - performance liquid chromatography (HPLC) showed that tow bacteria produced three types of rhamnolipids (mono - , di - rhamnolipid and rhamnolipid A). The rhamonlipid efficiency was tested for inhibitory activity against bacteria by measuring diameter of inhibitory zone surrounding holes and discs. The inhibitory activity was high against Bacillus cersus bacteria followed by P. aeruginosa, than Staphylococcus aeraus and the lowest one was E. colli. The inhibitory activity for biosurfactant was approached to inhibitory activity for industrial surfactants. Also in this study has been determined minimum inhibitory concentration (MIC) value and Minimum Bactericidal Concentration (MBC) value for biosurfactants and the results varied depending on different types of biosurfactants and type of bacteria and the lowest values for MIC and MBC of biosurfactant produced by P. aeruginosa PS42 on growth of B. cereus and reached to 16.It was first time at local level for testing inhibitory activity of biosurfactant against pathogenic bacteria S. aureus and P. aeruginosa which was studied in vivo (injuried skin) after introduction these pathogens experimentally into laboratory mice with clinical symptoms appeared in injured skin after 48 hours and then treated mice groups with of 30 mg \ ml concentration of biosurfactants produced from isolates of bacterial (P. aeruginosa PP8 and P. aeruginosa PS42), resulted in a decrease in the time required for healing as found from the results obtained by the different length of period needed for healing (depending on the nature of injury and type of microorganism that causes injury).The mice had been recovered after 5 days when used biosurfactant at concentration 30 mg/ml produced P. aeruginosa PS42 followed by mice recovered after 6 days when used biosurfactants 30 mg/ml produced by P. aeruginosa PP8 in comparison with control group which recovered after 12 days for mice infected with S. aureus. In mice infected with P. aeruginosa recovered after 10 days when used biosurfactant produced by P. aeruginosa PS42 at concentration 30mg/ml and followed by mice recovered after 12 days when used biosurfactant produced by P. aeruginosa PP8 at concentration 30mg/ml in comparison with control group which recovered after 17 days.
