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دراسة الطفرات الوراثية في جيني TPO وTSHR في محافظة البصرة

Author name: فله عبد الستار
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Plant - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Considered the thyroid glands task Deaf which produces important hormones are Tri iodothyrosine T3 and Thyroxin T4 and is responsible for their production a number of genes that encode the enzymes and proteins and the occurrence of any defect in this gene lead to the emergence of disorders of the thyroid work, including the case of palaces and fragments in hyperthyroid through the emergence of different pathological symptoms that indicate the occurrence of one of the cases studied in this research gland disorders at the level of molecular genetics .The study included only females were collected blood samples from 70 patients divided into 35 blood samples of the state of palaces and 35 blood samples for the case of the bulk in hyperthyroid deaf and diabetes endocrine centers of hospitals and ports Fayhaa year and Qurna, as well as from laboratory d. Nizar Mahfouz and represented a group of patients and 20 blood samples from healthy people represented as a set of control and that during the period from April to September 2013. But has been working on a sample of 70 patients (35 blood samples by the state of palaces and 35 blood samples for the case of the bulk gland) to add to the 20 blood samples of control and ages of the two groups and the healthy range (12 - 60 years).DNA was extracted DNA of two and three encoded amplified regions (8, 9 and 14) for the TPO gene and encoded region tenth TSHR gene PCR technology after it was diagnosed and analysis of mutations using the method for Sequences .The results showed the presence of two types of changes in the TPO and TSHR genes , two polymorphism (SNP) and Mutation, in gene156TPO study found hereditary forms c.1117G> T, A372S and c.1194G> C, S398T in the two groups of patients and healthy and not they relate to the occurrence patients palaces gland activity, as the study diagnosed 12 Novel Mutation in exon 8 in 21 patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all mutations diagnosed are mutations bitmap compensatory (substitution) the type of guarantee or Transtion Transversion depending on the nature of the base mutant, five of the mutations are Missense Mutation in 11 infected condition limitations are as follows c.949A>G,M317V , c.1064G>A,R355H, c.1277C>G,A425G , c.1063C>A,R355S and c.1207C>A,L402M and seven silent mutation in 10 patients hypothyroidism c.1062G>A,A354A , c.1050C>A,L350L , c.843C>A,A281A , c.1101G>A,V367V, c.1143C>T,I381I , c.1071G>A,R357R and c.2406C>T,D802D , While the results did not show any difference in the analysis of the genetic sequence of the two exons 9 and 14 at comparable with the genetic sequence of the control group. In this study suggests that there may be a correlation between the occurrence of mutations in a gene TPO and the state hypothyroidism . The results of the current study showed that the gene TSHR has an impact in both cases, and shortcomings in fragments in the activity of the gland where the study found three types of polymorphism (SNP) of the two of them Non - Synonymous type c.2181G> C, E727D and c.1489G> C, A496P and shape The last of the silent type genetic c.1377G> A, A459A, as the results showed that the two forms hereditary c.2181G> C and c.1489G> A have no correlation to happen disorders and are globally registered the shape genetic c.1489G> C, A496P did not score previously has current study for the first time and may have a link to happen one of the disorders depending on the genetic predisposition of the individual . I found the157current study, seven Novel Mutation in exon 10 distributed to five mutations among patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all the mutations diagnosed are mutations bitmap compensatory (substitutive Substitution) to ensure the kind of Transtion or Transversion depending on the nature of the base mutant 'three mutations are Missense Mutation in 3 people with the status of the palaces and the proportion of each mutation them (20%) are as follows c.1330 T>C , Y444H ; c.1424 T>C ,L475P and c.1435 T>C , S479P and two silent mutation in patients hypothyriodism c.1338 G>A, L446L and c.2300 G>A , Untranscrib , While the results recorded and there is only Tafrtin in the case of the bulk in hyperthyroid by (5.71%), one Missense mutation (c.1832 C> A, P610Q) and the other is silent (Silent) (c.2103 C> A, R701R). The present study indicated that the probability of the occurrence of the disease mutations link through the site mutations in specific areas to link hormone catalyst and the interaction between the Receptor and the unity of secondary alpha (G - protein). Results of the current study also showed that mutations identical and disparate factors have an impact on events in the case of deficiencies, genetic gland activity in the TPO and TSHR through defect events and constructed partially or wholly in the function of the protein encoded by genes TPO and TSHR

عزل وتشخيص مركب ايضي ثانوي جديد لكل من الفطرين Fusarium Trichoderma harzianum وsolani المعزولين من السواحل الرسوبية في محافظة البصرة وتقييم فعاليتهما الحياتية == Isolation and Identification of Novel Metabolic Compound From Both Fusarium solani and Trichoderma harzianum Isolated From Sedimentary Coast of Al - Basrah City and Estimate their Activity

Author name: فردوس نوري جعفر
Supervisor name: محمد لطيف علي | نيران جاسم الصالحي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: with the interest of medical and pharmaceutical uses of the natural products from marine fungi , thirty species of fungi belong to ninteen genus were isolated and identified from sea coast sediment of Al - Faw and Al - Seeba .The fungi Acremonium strictium , Geotricum candidum, Fusarium solani ,F.oxysporum , Penicilium sp. ,Trichoderma harzianum exhibited high inhibition activity toward growth of two reference strains of bacteria Escherichia coli (ATCC25922) and Staphylococcus aureus (NCtC 6571). The six selected fungi were grown in liquid culture media PDB at 25°C for two weeks ethyl acetate was used to extract the active compounds from thefermentation liquid media while ethanol was used to extract the active compounds from fungal mycelia .The crud extracts (fermentation and mycelium ) showed different inhibition activity against the pathogenic bacteria , Proteus mirabilis , S. aureus, isolates E. coli, Pseudomonas aeruginosa Klebsella pneumoniae. and pathoginic yeastes Candida albicans, C.tropicals,Cryptococcus neoformans and dermatophytic fungi Trichophyton verrucosum and Microsporium canis . The results revealed that the fermental fungi extracts has more inhibition activity than mycelial fungal extracts.The minimal inhibitory concentration (MIC) of fungal crude extracts were determined against gram positive and gram negative bacteria and pathogenic yeasts and fungi . antagonisum between studied fungi were evaluated , and the fungus T.harzianum has been the more antagonistic effect against other fungi. The toxicity of the fungal crude extracts were tested by using of human RBC at different concentration the result of this experiment showed that all extracts are non toxic except F.oxysporum extract.From all the above results the both F.solni and T.harzianum extracts showed higher activities ,thus these two extracts chosen for further studies. The anticancer activity of both extracts were studied against Hela cell line and the anticancer activity appeared at 250 Mg/ml and 62.5Mg/ml respectivily. The antioxidant activity of extracts were examined by three different methods ,B - carotene ,hydrogen peroxide scavenger and ferrus ion reduction , the results of three methods showed that both fungal extracts have antioxidant behaviours and the T.harzianum extract had more antioxident activity than F.solani extract and the antioxidant activity of both fungal extracts were increased as the concentration of the extracts increase.An optimization of the fungal growth and bioactivity of both fungi were conducted, by using different Nitrogen sources ( Asparagine , Yeast extract , peptone , Ammonium chloride , Sodium nitrate , Ammonium sulphate ) and carbon sources ( Maltose , Galactose , Glucose , Dextrose , Sucrose ,xylose and Starch ) the results elucidated that yeast extract was the best for growth of both fungi on solid and liquid media and high inhibition activity appear with T.harzianum against bacteria isolates , the carbon source starchenriched the growth of F.solani with more activity in inhibition the bacterial growth while the sucrose give high enrichment to the growth of T.harzianum in solid and liquid media with high activity toward bacterial growth. The study also indicates that the beast temperature and pH which gives the higher activity and growth 25°C and pH6 . The effect of different concentration of salinity were studied as well (1%,2%,3%,4%,5%,6% NaCl)and 2% showed the best concentration .The activity of two studied fungal extracts were superior than commercial antibiotic toward bacterial isolates .Preliminary qualitative chemical tests were carried out on the extracts and their chemical nature were identified .TLC technique were used and the chemical analysis showed that F.solani extract consist of two components with constant Rf values, the components marked as A1 and A2 . A1 shows higher activity against bacterial strains while T.harzianum extract consist of one component.The chemical structures of the isolated compound were

التشخيص المظهري والتغاير الوراثي لذباب القرعيات Dacus frontalis Becker ,1922 في محافظتي البصرة وميسان == Morphological identification and genetic variation of cucurbit fly Dacus frontalis Becker ,1922 in Basrah and Missan provinces

Author name: فاطمة قاسم حمدان
Supervisor name: فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study is identified (morphologic and molecular )for the population of cucurbit fly which is a destructive pest of cucumber ,honey melon and cantaloupe in Basrah and Missan .Sample were collected from different region from Basrah province including Qurna , Safwan , Zubair , Khor Al - Zubair . Al - Misharah , Kumate , Kalat Salih, Ali Al - Garbi in Missan province for the period October ,2014 till September 2015.Result of the identification study showed there is only one species infect cucurbits which is Dacus frontalis Becker ,1922,family Tephritidae ,Order Diptera in all studied regions. This species was classified according to femur color of the middle leg ,its color is orange at the apical half with dark black spot at the basal part of the antenna with the presence of basal diagonal line underneath the scutum behind the thorax. female were characterized by the presence of ovipositor at the end of the abdomen and male characterized by present s one line of hair at the third of the abdomen ring .For the sensitivity of the insect fly cucurbits towards our fruits of cucurbits (cucumber and water melon represented by the number of eggs laid down by the female as it appeared no significant difference in the number of eggs laid down by the female in the provinces of Basra, Maysan and was the highest rate of lay eggs on the fruits of cucumbers choice of six eggs and a difference of moral on water option, which amounted to one egg.Molecular study show the kit equipped Bioneer company and named Genomic DNA Extraction kit was highly efficient in DNA Extraction it was later used in the Random Amplified Polymorphic DNA - Polymerase Chain Reaction which is one of the methods used in molecular identification .Used four primers for different sequence s. and electrophoresis results shows multiplied bands of different molecular weight for four different primers for both females and males .The number and molecular weight of bands was calculated manually comparative with DNA marker or ladder then used the number of bands in the following equation to find the genetic similarity and genetic distance between the different districts each primer for males and females .To find genetic similarity used the equation : Sfy=2Nfy/Nf+ Ny and to findgenetic distance used the equation : GD fy=1 - SfyAt the level of first primer for females it was less value of genetic distance is zero and this is evidence that one species and highest value of genetic distance is 0.9 on the second level of the primer for females it was less value of genetic distance is 0.2 and highest value of genetic distance is one at the third primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one but the fourth primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one .For males at the level of first primer of males it was less value of genetic distance is zero and highest value is 0.8 but the second primer of males it was the less value for genetic distance is 0.8 and on the level of genetic distance is zero on the level of third primer for males it was value of genetic distance is zero and highest value is 0.8 and fourth of males it was the less value of genetic distance is zero and highest value of genetic distance is 0.8

دراسة تصنيفية وبيئية لبعض انواع عائلة ذباب الخيل Diptera : Tabanidae ودورها في نقل طفيلي Trypanosoma evansi في محافظة البصرة

Author name: علاء ناظم حاتم علي
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Zoology - Insects
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

دراسة الطفيليات الداخلية والخارجية في وعلى انواع مختلفة من الحشرات

Author name: علا عبد الشهيد ناصر
Supervisor name: كاظم صالح حسن الهدلك | مسلم عبد الرحمن الطعمة
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: Present study was carried out to isolate and identify the worms nematodes found in the soil, and could be used as a means of biological control against insects, it has also been to isolate and identify Mites present on or within different types of economic and other insects, which were represented by the American cockroaches Periplaneta americana ( Dictyoptera; Blattiidae). And houseflies Musca domestica (Diptera : Muscidae), Persian and ants belonging to the family; Formcidae) Hymenoptera), flour beetles Tribolium castaneum (Tenebrionidae; Coleoptera), and the termites Microcerotermes diversus( Isoptera; Kalotermitidae))The study samples (soil and insects)were collected from different regions and ecosystem of the province of Basra, during the period of the month of December 2014 to the month of September 2015. By setting traps, appropriate and specifically designed for this purpose, that supplied with special food to attract nematodes in the soil and catch insects found in those ecosystem, . and also to determine the best ways to isolate samples preserved and propagate them..Also, the study dealt with experiments for the propagation of the mites (which collect from insect samples) in the laboratory,by growth them on different growth media, for the purpose of immunological and genetic study, and the ability to be used in bio resistance and also to determine the sensitivity of the laboratory mice, that attacked by the mites through conducting hitological study of the skin of these animals..Results recorded two types of endo parasitic nematodes these areFamily : Steinernematidae1 - Steinernema feltiae2 - S. carpocapsaeAnd five species of mites, these areFamily : Histiomatidae2 - Histiostoma sp1 Family : Acaridae 1 - Cosmoglyphus barbisetusgrifolapholiotae 2 - Mycetosancassania Family : Macrochelidae Vitzthum, 1930 Macrocheles muscaedomesticae Family : Pyemotidaepymephorus tarsalis

تاثير بعض مستخلصات الطحلب الاخضر Chara vulgaris L. على البيوض والطور اليرقي لشريطية القطط Taenia taeniaeformis Batsch, 1786 == The Effect of Some Green Algae Extracts Chara vulgaris L. On The Eggs and The Cysticercus of Taenia taeniaeformis Batsch, 1786

Author name: عقیل عبد الصاحب عبد الحسین الوائلي
Supervisor name: باسم ھاشم عبد الله | عماد یوسف عواد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:

التغيرات الكيميوحيوية المصاحبة للشد الملحي وتقييم دور مستخلص الطحلب الاخضر Cladophora crispata في نبات الرز Oryza sativa L. المستزرع نسيجيا

Author name: عبد الامير رحيم عبيد
Supervisor name: حسيي خلف زاير | لوى حسين عبد القادر
General topic: Biology
Specific topic: Plant - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted at the tissue culture laboratory at the Dept. of biology, College of Education pure Sciences from 1/3/2012 - 1/12/2014.With the objective of studing the tolerance of the salt stress of two rice (Oryza sativa L.)cultivars namely Anber and Furat using the technology of tissue culture. . Mature seeds (mature embryo and endosperm) were used as bing parts of explants which were cultivated in a semi - sold( Murashige and Skoog 1962) nutrient medium and some additives such as vitamins and amino acids .The growth regulator Dichlorophenoxy acetic acid (2.4 - D)was used, about 8mg/l , sucrose at 30g.l - 1 . . The study ,also ,tested the effect maltose at 30g.l - 1, and the effect of 0.5g/l of casein hydrolest were tested.the study also included the different of sea water concentrations (0,20,40,60)%on the induction of growth and development of callus ,the effect of green algae extract at Cladophora crispate by adding 1g fresh weight to the nutrient medium with all sea water concentrations.All the features of the callus were tested as well as the effect of the interaction between sea water concentrations and algae extract , as well as interactions among rice cultivar x sea water concentration x algal extract.The study depended on some growth indicators to determine the degree of callus to tolerance towards salt stress .the resistance of callus to salt depended on regeneration through the required of time for the formation of embryos ,number of germinated embryos ,and number of plantlets .The concentration of the major metal ion : sodium, potassium, phosphorus, calcium ,magnesium, potassium/sodium percentage , and the minor metal ions : iron ,copper, manganese were also some chemical components such as protines and amino acid proline measured, were also measured .The randomized block design (C.R.D)and factorial experiments were used depending ten replicates per treatment .The results can be summarized as follows : 1 - The results showed that the addition of sugar di maltose as an energy source and Casein hydrolyses to the nutrient medium had appositive effect on significant decrease in the required time to mitiate the callus ,in the increase of callus induction percentage and in the increase of freshweight of initial callus. .2 - The effect of sea water and algae extract : . A - The current study shaued that the addidtion of 20 and 40 % sea water to the nutrient medium resulted in a significant increase in the required time to induce the callus ,and a significant decrease in the percentage of callus induction and weight of the mitial callus as compared with the control treatment .The concentration 60%0f sea water caused the mature seeds to lose their ability to germinate and induce the callus if no induction occurs at all . The effect of algae extract resulted in a significant decrease in the required time for callus induction ,and a significant increase in the percentage of callus induction ,the weight of the mitial callus and 0,20,40% of sea water as compared with free algae extract medium.B - The results showed that the addition of 20% of sea water to the nutrient medium that is prepared for the growth of the embryo callus led to a significant increase in the fresh weight as compared with the control treatment , whereas the two concentrations 40 and 60%sea water led to a significant decrease in the embryo callus weight .The effect of the algae extracted to a significant increase in the fresh weight of the embryo callus with sea water concentrations as compared with the free algae extract medium.C - The addition of 20,40 and 60%sea water cause a significant decrease in the number of the somatic embryos ,thir germination auerage ,number and germination of the formed plantlets . This addition also caused a significant increase in the required time to stare the germination of the somatic embryos as compared with the control treatment .The addition of the algae extract led to increase the number of formed embryos ,germination percentage ,and number and percentage of plantlets ,wher was the addition led to lower the required time to germination the embryos compared with the free algae extract medium.D - The addition of 20 ,40 and 60% sea water to the nutrient medium resulted in a significant increase in the concentration of sodium ,calcium ,and magnesium ion in the embryo callus ,while a significant decrease occurred in the concentration of potassium and phosphorus ion and thedpotassium/sodium percentage as compared with the control treatment .The abone mentioned concentrations 0f sea water led to significant increase in concentration of ion ,copper, and zinc ions ,and significant decrease in manganese ion ,The addition of the algae extract caused in lowering the concentration of sodium ion .This decrease was significant in the 60 % sea water medium. The ingrease of potassium ,calcium ,phosphorus, and magnesium ion in embryo callus tissue when compared with free algae extract medium and was significant with some sea water concentration.E - The results ,also ,showed that the addition on 20, 40 and 60% concentrations0f sea water resoled in a significant decrease in concentration of some chemical ingredients such as protein and amino acid proline of the embryo callus in comparison with the control treatment . as far as the effect of the algae extract is concerned ,the addition of this extract to the nutrient medium led to a significant increase in the concentration of the proteins and amino acid prolne as well as with all sea water concentrations.F - The results of study showed that the addition of 20,40,60% sea water led to a significant increase in the rate 0f effectiveness 0f the enzyme Super oxide dismutase (S.O.D) and enzyme Ascorbate peroxides (APX),reaching the highest rate of effectiveness of the enzyme at the concentration 0f 60% compared with the comparative treatment , whil the highest average of the effectiveness of enzyme Catalase (CAT)and glutathione piroxidase (GP)and polyphenoloxidase (ppo)was at the concentration 20%sea water then it started to decline when increasing the concentration of sea water from 40 to 60% As for the algae extract it has been shown from the results of the current study that the addition of algae extract to nutrient medium to a high effectiveness of enzyme SOD,CAT ,APX and GP and more significant with all concentration of sea water The impact of algae extract on the effectiveness of the enzyme PPO has led to increasing the effectiveness of this enzyme but did not amount to a significant level except with sea water nutrient medium .G - The results of the current study showed a significant increase of the Amber cutivar in the percentage of the callus induction and the required time of this induction as well as the Wight of the initial and embryo callus ,number and germination percentage of thesomatic embryo , number of plantlets ,as wall as the percentage of the accumulation of potassium ,phosphorus ,calcium, and manganese ions ,as will as in the potassium/sodim ratio focus histidine proline and the effectiveness of the enzyme SOD and CAT enzyme and enzyme GP. While the product was higher than in the accumulation of Firat magnesium ion ,zinc copper and in the effectiveness of the enzyme in APX & PPO . .

دراسة وبائية وجزيئية للجرثومة Escherichia coli O157 : H7 المعزولة من عينات سريرية وبيئية في محافظة البصرة - العراق == Epidemiological and molecular study of the bacterium Escherichia coli O157 : H7 isolated from clinical and environmental samples Basra Governorate / Iraq

Author name: عباس ضرب شعبان
Supervisor name: ميثم ايوب عبد القادر الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 464 clinical and environmental samples attempt to isolate Escherechia coli O157 : H7 serotype were collected, of which 183 stool samples from diarrheal children at Al - Qurna hospital and Al - Medaina Hospital of children during the period between October 2013 - August 2014. Environmental samples (281) : 121 samples of drinking ( tap) water , 80 samples of river water , and 80 samples of sewage water (collected from different regions of Basra Governorate) . All samples was tested and detected the presence of Escherichia coli serotype O157 : H7 using chromogen agar and ( CT - SMA ) as selective media and using direct immunological testing for direct identification of the pathogen (Certeste) for clinical samples.The study revealed the isolation of 56 isolates of E.coli O157 : H7 at a rate of (12.06%) : 16 clinical isolates at a rate of (8.7%), 40 environmental isolates at a rate of (14.23) : 9 isolates from drinking water at a rate of (7.4%), 18 isolation from sewage at a rate of (22.5), and 13 river isolates at a rate of (16.25).Antibiotic susceptibility testing toward 20 antibiotic of E.coli O157 : H7 isolates showed that all isolates from all sources resistant to the antibiotics Ampicillin, Amoxacilllin, , Rifampin and Piperacillin while showed high sensitivity to antibiotics amikacin, Kanamcin, ciprofloxacin and norfloxacin.The clinical isolates of E.coli O157 : H7 showed multiple high antibiotic resistance index Muliple Antibiotics Resistance (MAR index) ranged between (0.25 - 0.8), Where two isolates showed a resistance to sixteen antibiotics, Two isolates showed resistance to twelve antibiotics, Two isolates showed a resistance to eleven antibiotics, one isolate showed a resistance to ten antibiotics,Three isolates showed a resistant to nine antibiotics and two isolates showed aresistance to eight antibiotics, One isolate showed a resistant to six antibiotics and two isolates showed resistance to five antibiotics.The environmental isolates of E.coli O157 : H7 showed less resistance ranged between (0.25 - 0.75) for samples of sewage and (0.35 - 0.50)rivers samples , and (0.30 - 0.55)tap water samples . As shown, one isolate resistant to fifteen antibiotics, one isolate showed a resistance to thirteen antibiotics and four isolates showed a resistance to ten antibiotics from sewage. As for rivers Tow isolates showed a resistance to ten antibiotics, three isolates showed a resistant to nine antibiotics while a one isolate of tap water showed a resistance to eleven antibiotics, three isolates showed a resistance to ten antibiotics and an one isolates showed a resistance to nine antibiotics and two isolates resistant to eight antibiotics.Investigating about the content of plasmid in isolates E.coli O157 : H7 to study the relationship between the plasmid content as an indicator of antibiotic resistance, as the results showed that most of the isolates E.coli O157 : H7 four clinical isolates containing the plasmid including one molecular weight > 10kbp with a Patterns of multiple different resistance,also two isolates contain three plasmids of a molecular weight > 10kb. And one isolate containing four plasmids with molecular weights ranging from (4 - 10Kb) and one isolate containing five plasmids resistant to sixteen antibiotics with molecule weights ranged between >(2 - 10Kb).The isolates E.coli O157 : H7 isolated from the environment , sixteen isolates showed that they contain plasmids of molecular weights> 10kb , one isolate contains two plasmids of molecular weights ranging between (4 - 10Kb), five isolates containing three plasmids of molecular weights ranging from (2.9 - 10) Kb and two isolates containing four plasmids of molecular weights ranging between( 2 - 10 Kb

دراسة بعض التاثيرات الفسيولوجية والنسجية لدواء Paclitaxel المضاد للسرطان في الفئران المختبرية Mus musculus L. == Study of some physiological and histoloygical effects of the anticancer drug paclitaxel in laboratory mice Mus musculus L

Author name: ضحى طالب حميد الطائي
Supervisor name: سامي جبر المالكي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: صممت الدراسة الحالية لمعرفة تاثير دواء Paclitaxel المضاد للسرطان في الجوانب الفسيولوجية والكيموحيوية والنسجية في الفئران المختبرية البيض Mus musculus L. , اذ تم تقسيم ذكور الفئران المختبرية واناثها الى ثلاث مجاميع , تتكون كل مجموعة من ثمانية فئران هي : مجموعة السيطرة Control group : تتكون من 8)) ذكور و(8) اناث , حقنت بـ (0.2) مل من المحلول الفسيلوجي Normal Saline داخل الغشاء البريتوني (I.P) .مجموعة الجرعة الواطئة : تتكون من (8) ذكور و(8) اناث, حقنت بـ (0.2) مل من محلول دواء Paclitaxel بتركيز 12 ملغم / كغم داخل الغشاء البريتوني (I.P) .مجموعة الجرعة العالية : تتكون من (8) ذكور و(8) اناث , حقنت بـ (0.2 ) مل من محلول دواء Paclitaxel بتركيز 24 ملغم /كغم داخل الغشاء البريتوني (I.P) . وقد اوضحت نتائج الدراسة الحالية حدوث انخفاض معنوي في معايير الدم المتضمنة تعداد كريات الدم الحمر, وحجم الدم المضغوط وخضاب الدم في كل من ذكور الفئران المختبرية واناثها المعاملة بالجرعتين ( 12 و24) ملغم /كغم وانخفاض معنوي في معدل حجم الكرية الحمراء بالجرعة العالية في الاناث عند مقارنتها بمجموعة السيطرة . كما بينت النتائج انخفاضا معنويا في التعداد الكلي لخلايا الدم البيض والنسبة المئوية للخلايا الوحيدة والحبيبية في ذكور الفئران المختبرية المعاملة بعقار Paclitaxel بالجرعتين (12 و24) ملغم \كغم بينما لوحظ في الاناث انخفاض معنوي في العدد الكلي لخلايا الدم البيض والنسبة المئوية للخلايا الوحيدة بالجرعتين والحبيبية بالجرعة العالية 24 ملغم \كغم عند مقارنتها مع مجموعة السيطرة, وارتفاع معنوي في النسبة المئوية للخلايا اللمفاوية بالجرعتين 12)و 24) ملغم \كغم لذكور الفئران المختبرية واناثها. اظهرت النتائج وجود انخفاض معنوي في وزن جسم ذكور الفئران المختبرية واناثها بالجرعة العالية 24 ملغم / كغم فقط عند مقارنتها مع مجموعة السيطرة. كما اوضحت النتائج حدوث انخفاض معنوي في نسبة خصوبة ذكور الفئران المختبرية واناثها (عدد الحوامل) المعاملة بعقار Paclitaxel بالجرعتين ( 12و24 ) ملغم / كغم , وحدوث انخفاض معنوي في عدد مواليد الامهات والذكور المعاملة بالجرعتين , كما اظهرت النتائج انخفاضا معنويا في اوزان المواليد عند اليوم الاول للولادة والاسابيع الخمسة بعد الولادة في ذكور الفئران المختبرية المعاملة بالجرعتين بينما لوحظ في الاناث المعاملة بالجرعتين (12 و24 ) ملغم \كغم انخفاض معنوي في اوزان الولادات عند اليوم الاول للولادة والاسابيع الثلاثة بعد الولادة وبالاسبوع الخامس بالجرعة العالية عند مقارنتها بمجموعة السيطرة . سبب دواء paclitaxel انخفاضا معنويا في اعداد الحيامن بالجرعتين 12)و (24ملغم /كغم وخلال المدتين 45, 30 يوما , بينما لم تظهر النتائج فرقا معنويا في تشوهات ذيل النطف ورؤوسها بالجرعتين وخلال المدتين عند مقارنتها مع مجموعة السيطرة , فضلا عن ذلك بينت النتائج حدوث انخفاض معنوي في اعداد الحيامن السوية بالجرعتين وخلال المدتين. اظهرت نتائج الدراسة الهرمونية انخفاض معنوي في مستوى هرمون الشحمون الخصوي والهرمون المحفز للجريبات stimulating hormone (FSH) Follicle والهرمون اللوتيني Luteinizing hormone (LH) بالجرعتين في ذكور الفئران المختبرية, بينما لوحظ في الاناث انخفاض معنوي في هرمون الاستروجين بالجرعة العالية 24 ملغم /كغم , وانخفاض في الهرمون المحفز للجريبات بالجرعتين عند مقارنتها مع مجموعة السيطرة . كما بينت نتائج الدراسة الحالية وجود ارتفاعا معنويا في مستوى انزيمات الكبد المتمثلة بانزيم Aspartate transaminase ( AST) و(Alanine transaminase ( ALT وAlkaline phosphatase (ALP) بالجرعة العالية 24 ملغم / كغم في ذكور الفئران المختبرية عند مقارنتها مع مجموعة السيطرة . كما اظهرت نتائج الدراسة ايضا انخفاض معنوي في مستوى البروتين الكلي في ذكور الفئران المختبرية واناثها عند معاملتها بالجرعة العالية 24 ملغم / كغم فقط مقارنة مع مجموعة السيطرة. اشارت نتائج الدراسة الحالية الى ارتفاع معنوي في مستوى الدهون المتعادلة ثلاثية الكليسريد بالجرعة العالية في كلا الجنسين وانخفاضا معنويا في مستوى الدهون المتعادلة ثلاثية الكليسريد في ذكور بالجرعة الواطئة 12 ملغم/كغم وارتفاعا معنويا في مستوى الكولسترول الكلي لمصل الدم في الذكورفقط بالجرعة العالية 24 ملغم /ملغم وارتفاع معنوي في مستوى البروتين الدهني عالي الكثافة High density lipoprotein في ذكور الفئران المعاملة بالجرعة العالية 24 ملغم \كغم وبالجرعة الواطئة 12 ملغم \ كغم في الاناث , كما سبب الدواء انخفاضا معنويا في مستوى البروتين الكلي واطئ الكثافة جدا Very low density lipoproteinفي الذكور بالجرعة الواطئة عند مقارنتها مع مجموعة السيطرة . واظهر حقن دواء Paclitaxel في الفئران المختبرية حدوث ارتفاع معنوي في مستوى اليوريا لمصل دم ذكور بالجرعتين ( 12 و24 ) ملغم\كغم , بينما في الاناث بالجرعة العالية عند مقارنتها مع مجموعة السيطرة . واظهرت نتائج دراسة المقاطع النسجية في خصى الفئران المختبرية المعاملة بدواء Paclitaxel حدوث تحلل كامل في جدار معظم النبيبات المنوية وفرط تنسج في خلايا ليدك بالاضافة الى نزف في النسيج الضام البيني , وخلل تنسج Dysplasia . وبينت المقاطع النسجية في مبيض الفئران المختبرية المعاملة بالدواء حدوث احتقان بعض الاوعية الدموية , وتحلل خلية البيضة , وتنخر خلايا الطبقة الحبيبية وانفصالها عن خلية البيضة وعدم تكون طبقة خلايا الاكليل المشع Corona radiate بالاضافة الى تحلل نواة الحوصلة الثانوية . كما سبب دواء Paclitaxel تغيرات مرضية نسجية في كبد وكلى الفئران المختبرية , اذ اظهرت نتائج المقاطع النسجية لكبد ذكور الفئران واناثها المعاملة بالدواء حدوث تنخر وتنكس في الخلايا الكبدية ,وارتشاح الخلايا الالتهابية مع ظهور تشوه وتضخم انوية بعض الخلايا الكبدية وفرط تصبغ السايتوبلازم ,بالاضافة الى توسع الوريد المركزي والاوعية الدموية ,وتوسع الوريد البابي الكبدي واشباه الجيوب الكبدية واحتقانها . اظهرت الدراسة النسجية لكلى ذكور الفئران المختبرية واناثها المعاملة بالدواء حدوث تنخر بطانة النبيبات وتنكسها , وارتشاح في الخلايا الالتهابية مع ظهور تشوهات في الانوية وحدوث موت خلوي مبرمج , واحتقان في الاوعية الشعرية الدموية, وتوسع تجويف النبيب الملتوي القريب واختفاء حافته الفرشاية, وتوسع الاوعية الشعرية الدموية, ونزف وتضخم وفرط تنسج وتوسع, وتضيق حيز محفظة بومان في الكبيبة , وحؤول بطانة النبيبات, وانتفاخ بعض خلايا النبيبات الكلوية وتضخمها . | This study was designed to investigate the effects of the anticancer drug Paclitaxel on some physiological, Biochemical parameters and histopathological changes in albino laboratory mice Mus musculus L. , The male and female laboratory mice were divided into three groups, eight mice each : 1 - The control group (8) males and (8) females , all of them injected with (0.2)ml of physiological saline Intraperitoneal (I.P) .2 - The low dose group of (8) males and (8) females, all of them injected with (0.2) ml of 12 mg/kg paclitaxel , Intraperitoneal (I.P) . 3 - The high dose group of (8) males and (8) females, all of them injected with (0.2) ml of 24 mg/kg paclitaxel , Intraperitoneal (I.P) . The results of this study showed a significant decrease (P≤0.05) in blood parameters that include red blood corpuscle (RBC) , packed cell volume (PCV) and haemoglobin (Hb) for both male and female mice were injected with two doses of paclitaxel (12,24) mg/kg and a significant decrease in mean cell Volume (MCV) that are treated with high dose. The results also showed a significant decrease in the total number of white blood cells and percentage of monocyte and granulocyte cells in male mice treated paclitaxel for both two doses (12,24) mg/kg . while in female mice the total number of white blood cells and percentage of monocyte cells were significant decreased with two doses and percentage of granulocyte cells with high doses 24 mg\kg in comparison with the control group, and a significant increase in percentage of lymphocyte in male and female mice were treated with the two doses(12,24) mg/kg. The results also exhibited a significant decreased in body weight of male and female mice in high dose 24 mg/kg only when compared with control group. The results exhibited a significant decrease in fertility of male and female mice treated with paclitaxel with two doses (12 ,24) mg\kg, there was a significant decrease also in number of newborns male and female treated with two doses, and there was a significant decrease in newborns weight in the first day of birth and the five weeks of birth with (12,24) mg/kg , while female treated with the two doses , there was a significant decrease in the first day of birth and subsequent three weeks after birth with the two dose and fifth week with the high dose ( 12 , 24) mg/kg in comparison with the control group . The results of this study also showed a significant decrease in sperm count treated in mice with the two doses after 30 - 45 days of birth . while no significant difference in malformation rate of sperm (head and tail) with the two doses through the same period in comparison with the control group . there was a significant decrease in the number of normal sperm through the same period with the two doses . The hormonal study showed a significant decrease in testosterone level and follicle stimulating hormone (FSH) with both doses and luteinizing hormone (LH) level with two doses for male mice , in females there was a significant decrease in estrogen levels with high dose 24 mg\kg and a significant decrease in follicle stimulating hormone (FSH) with two doses in comparison with control group. The present study showed a significant decrease in levels of liver enzymes Aspartate transaminase (AST) , Alanine transaminase (ALT) and Alkaline phosphatase (ALP) in male mice were treated with high dose (24) mg/kg in comparison with control group . The present study also showed significant decreased in total protein in male and female mice treated with high dose (24) mg/kg only in compared with control group. The study showed a significant increase in triglycerides level in male and female mice that are treated with high dose and it also showed a significant decrease in triglycerides level in male mice with low dose 12 mg/kg .There is a significant increase in the total cholesterol level for serum in male mice only that are treated with high dose 24 mg/kg .The study showed a significant increase in high density lipoprotein (HDL) in male mice with high dose 24 mg/kg and even with the low dose 12 mg\kg in female mice . The drug causes a significant decrease in very low density lipoprotein (VLDL) in male mice that are treated with the low dose in comparison with the control group . Injection with paclitaxel in mice lead to a significant increase in urea level in serum for male mice that are treated with both doses (12,24) mg\kg in comparison with the control group. The results of the study of histological sections in male testis were treated with paclitaxel showed analysis in most seminiferous tubules , hyperplasia in leydig cell , bleeding in interstitial connective tissue and dysplasia. Histological sections in female mice ovary were treated with paclitaxel showed a congestion in some blood vessel , oocyte degeneration, necrosis of granular layer and separated from oocyte and degeneration of secondary follicle nuclei. Injection with paclitaxel leads to histopathological pathological changes in mice kidney and liver .The result of histopathological sections of liver in male and female mice were injected with paclitaxel showed necrosis and degeneration in hepatocytes, infiltration of inflammatory cells with show malformation and hypertrophy of some hepatocytes nuclei , hyperchromic cytoplasm, also dilation in central vein and blood vessel , dilation and congestion of portal hepatic vein and sinusoids. The study of histological sections for male and female kidneys were treated with paclitaxel showed necrosis of endothelial tubules and infiltration of inflammatory cells with show malformation in nuclei and apoptosis , congestion in blood vessel , dilation in proximal distal tubules and absent of brush border , dilation capillaries , bleeding, hypertrophy , hyperplasia, dilation and narrow of bowman's capsule , metaplasia endothelial tubules , swelling cells and hypertrophy of some renal tubules cells .

تقدير الهستامين في بعض الاغذية المحلية وتحديد جينات الهستدين (hdc) في البكتريا المعزولة منها ودورها في تراكم الهستامين == Estimation of histamine in some local food and detection of histidine genes (hdc) from their isolated bacteria and it’s role in accumulation of histamine

Author name: صلاح ناجي عزيز الخيون
Supervisor name: قيثار رشيد مجيد | طالب احمد جايد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: ELISA technique was used to estimate of histamine in muscles of several types of local fresh fish, including : Tenualosa ilisha, Megalaspis cordyla , Chirocentrus dorab and Scomberoides commersonianus in addition of the shrimp Metapenaeus affinis , all samples of fish and shrimp were collects from Fao markets in Basarah city .Results showed the percentages of histamine in all types of fish, as well as shrimp were : 4.483 and 3.953 and 3.353 and 3.16 and 2.03 mg/kg respectively.Accumulation of histamine in fish after refrigerating for three days was also determinated. Results showed that the values of histamine accumulate were increased within preservation period .On the third day of histamine accumulate of C. dorab , T. ilisha , M. cordyla , S. commersonianus and shrimp were 4.56 , 3.99 , 3.43 , 3.19 and 2.01 mg / kg, respectively. While , on the first day were 4.01 , 3.65 , 3.03 , 2.98 and 1.89 mg/kg respectively .ELISA technique was also used to estimate histamine in Ripening cheese such as cheddar , Alkuda , Almoszerla and Oloadam cheese and smoked Hajdu . They were 4.267 , 2.433, 1.167 , 3.667 and 1.833 mg / 100 g , respectively.Many bacteria were isolated isolated from fishes and shrimp, they identified according to : growth characteristics on selective media, morphological, and microscopic examinations in addition to biochemical tests.Fifty three isolates of bacteria were found, they were : Klebsiella pneumonia sub. pneumonia , Klebsiella oxytoca , Morganella morganii sub. Morganii, Enterobacter aerogenes, Enterobacter cloacae , Cronobacter sakazakii ,AEnterobacter taylorae, Citrobacter freundii , Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei , Escherichia coli, Serratia marcescens, Serratia fonticola , Vibrio vulnificus, Vibrio diazotrophicus , Vibrio hollisae , Vibrio alginolyticus, ,Vibrio cholera, Vibrio parahaernolyticus, Vibrio fluvialis, Lactobacillus helveticus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus johnsonii, Lactobacillus salivarius, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus saprophyticus, Staphylococcus aureus , Staphylococcus intermedius , Staphylococcus epidermidis, Bacillus pumilus , Bacillus licheniformis , Bacillus firmus , Bacillus subtilis, Bacillus alvei , Aeromonas. caviae , Aeromonas veronii bv.veronii , Aeromonas eucrenophila, A. sobria , Aeromonas veronii and Aeromonas encheleia .Isolation and identification of Lactobacillus from all samples of ripening cheese were done. Six species of Lactobacillus were found and as a follow : Lactobacillus plantarum , Lactobacillus delbrueckii, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum and Lactobacillus paracasei .DNA was extracted from all types of G+ and G - bacteria which isolated from fish and shrimp in addition to Lactobacillus bacteria that isolated from ripening cheeses . the DNA of all bacteria was good and pure .Polymerase chain reaction (PCR) technique was used for identification all isolates of Lactobacillus which were isolated from fish and shrimp, as well as ripening cheese depending on the bacterial 16S rRNA gene sequences of bacteria with using different types of diagnosed primers, genes had been identified for : Lactobacillus acidophillus , Lactobacillus plantarum , Lactobacillus delbrueckii , Lactobacillus fermentium , Lactobcillus helveticus , Lactobcillus crispatus ,BLactobcillus gasseri, Lactobcillus reuteri , Lactobcillus rhamnosus, Lactobcillus paracasei, Lactobacillus johnsonii , Lactobacillus salivarius and Lactobacillus casei .Nine primers were in RAPD technique to find out the genetic link between different strains of Lactobacillus bacteria, the results showed that the number and location of randomized bands were differed in some bacterial species but others was fitted and produced a variety genetic of bacterial strains, in particular a number of bands as possible to get a variety of different genetic profiles among Lactobacillus isolates .The results appeared different styles of bands and presence of specific bands in Lactobacillus bacteria in all primers . The results obtained from RAPD technique showed that the total number of bands were 630 bands, the total number of bands which formed genetically was 127 and the percentage of total proportion of genetic variation among the isolates was 20.15% .The results found of genetic primers scheme that the genetic distance was as close as possible between bacteria L.fermentum and L.rhamnosus and was a part of the one genetic group, but L.helveticus was close as possible genetically to this group , and then the L.johnsonii and L.acidophilus was more genetically distant to this group. Also it found that the genetic distance was very near between L.plantarum and L.paracasei and were a part of a one hereditary group and found also that L.reuteri closest genetically to this group, then followed by more genetically L.delbrueckii . However, L.acidophilus was also more genetically distant to this group .CThe results revealed that L.salivarius and L.crispatus were a one hereditary group and the genetic distance for this group was very close to the L.gasseri and it was farther genetically for genetic group, which includes L.fermentum and L.rhamnosus , also found that the bacteria L.casei showed far genetic distance between them and the groups of Lactobacillus .All fifty three isolates of bacteria which isolated from fish , shrimp and ripening cheeses that producing and did not producing histamine subjected to amplified genes which responsible of the histamine production by using the primers HIS1 - F/HIS1 - R ; JV16HC/ JV17HC ; Hdc - f/ Hdc - r ; 106/107 and UNI - L/ UNI - R . Amplification results showed that all primers used to amplified genes of histamine gene different genes sizes depending on the types of bacteria when were positive or negative to Gram stain and their OrigenIt was found the size of the gene was 350 base pairs , was found in G+ bacteria which isolated from fish , shrimp and cheese ripening . These bacteria included : L.acidophilus ,L.helveticus ,L.crispatus, L.gasseri ,L.casei , L.reuteri , L.fermentum,L.rhamnosus, L.paracasei, L.johnsonii, L.salivarius L.plantarum , L.delbrueckii , S.aureus , S. saprophyticus , S.epidermidis , S. intermedius , Bacillus pumilus, B.subtilis, B.licheniformis, B.firmus and B. alvei .May same results were found when amplified HISI - R/HISI - F primer , the size of histamine gene most positive Gram bacteria stain .High size of histamine gene 709 base pair appeared when amplified Hdc - r/ Hdc - f primer .This gene was found in G - bacteria which isolated from fish and shrimp . These bacteria included : Pseudomonas aeruginosa , Pseudomonas fluorescens, Klebsiella pneumonia , Morganella morganii , Enterobacter aerogenes, KlebsiellaDoxytoca, Hafnia alvei, Enterobacter cloacae, Vibrio alginolyticus, Cronobacter sakazakii, Escherichia coli , Enterobacter taylorae, Proteus vulgaris, Proteus mirabilis, Vibrio vulnificus , Vibrio diazotrophicus, Vibrio hollisae , Vibrio fluvialis , Serratia marcescens and Serratia fonticola .However , the size of histamine gene was 530 base pairs , when amplified 106 / 107 primer , the gene was found in most G - bacteria .On the other hand , the UNI - L/UNI - R primer did not amplify in all isolates of bacteria which isolated from fish , shrimp and ripening cheeses .Extraction of plasmids from all isolated bacteria was done . Results showed that isolates have different types of plasmids bands (small and large size) and when the electric deportation of the genetic material of plasmids on gel agarose while some other isolates appeared possess one plasmid.In this study , PCR technique was not detected the presence of histamine genes in plasmid of bacteria , because not occurred any amplify for any type of primers used . This indicates the presence of histamine genes on the genome of the bacteria and not on plasmids of bacteria .

دراسة بعض التاثيرات الفسيولوجية والنسجية لمبيد دايمثويت في الفئران المختبرية البيض Mus musculus L. == Study of some Physiological and Histological Effects of Dimethoate pesticide in laboratory white mice Mus musculus L

Author name: صفا محمد حسين
Supervisor name: علي عبد اللطيف العلي | فارس شاكر كاطع
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تم دراسة التاثيرات الفسيولوجية والنسجية في بعض اعضاء الفئران المختبرية بعد حقنها بجرعتين من المبيد الحشري الفوسفوعضوي دايمثويت Dimethoate, اذ قسمت ذكور الفئران المختبرية الى ثلاث مجاميع وضمت كل مجموعة ثمان فئران وبالطريقة نفسها قسمت اناث الفئران وحقنت مجاميع الذكور والاناث كالاتي : - 1مجموعة السيطرة : حقنت بحجم 0.1 مل من المحلول الفسيولوجي .Normal saline 2 - مجموعة المعاملة الاولى Treated group 1 (T1) : حقنت بحجم 0.1 مل من محلول مبيد dimethoate وبتركيز 6ملغم/ كغم ولمدة 30 يوما ومثلت الجرعة الواطئة من المبيد. - 3 مجموعة المعاملة الثانية Treated group 2 (T2) : حقنت بحجم 0.1 مل من محلول مبيد dimethoate وبتركيز12 ملغم/ كغم ولمدة 30 يوما ومثلت الجرعة العالية من المبيد. بينت نتائج المعايير الدمية لذكور الفئران المختبرية وجود انخفاض معنوي في كل من عدد كريات الدم الحمر وخضاب الدم وحجم الدم المضغوط وبالجرعتين 6 و12 ملغم/ كغم مقارنة بمجموعة السيطرة, كما اظهرت النتائج انخفاض معنوي في معدل تركيز خضاب دم الكرية MCHC وبالجرعة 12 ملغم / كغم فقط ، اما في الاناث فقد انخفض معنويا عدد كريات الدم الحمر وحجم الدم المضغوط ومعدل حجم الكرية ومعدل تركيز خضاب دم الكرية وبالجرعتين6 و12 ملغم/ كغم عند المقارنة بمجموعة السيطرة, في حين انخفض معنويا معدل خضاب الدم Hb بالجرعة الواطئة 6 ملغم/ كغم فقط عند مقارنتها بمجموعة السيطرة. اظهرت نتائج العد التفريقي لخلايا الدم البيض في ذكور الفئران انخفاضا معنويا في عدد خلايا الدم البيض الكلي وبالجرعتين6 و12 ملغم/ كغم، كما انخفضت معنويا كل من النسبة المئوية للخلايا الحبيبية والوحيدة بالجرعة العالية 12 ملغم/ كغم فقط عند مقارنتها بمجموعة السيطرة ,في حين انخفض عدد خلايا الدم البيض الكلي والنسبة المئوية للخلايا الحبيبية في اناث الفئران المحقونة بالجرعة 12 ملغم/ كغم فقط من المبيد عند المقارنة بمجموعة السيطرة. اوضحت نتائج الدراسة الحالية وجود انخفاض معنوي في اعداد الحيامن بالجرعتين6 و12 ملغم/ كغم, ووجود ارتفاع معنوي في نسبة تشوهات رؤوس الحيامن وذيولها فضلا عن وجود انخفاض معنوي في اعداد الحيامن الطبيعية وبالجرعتين 6 و12 ملغم/ كغم مقارنتها بمجموعة السيطرة. بينت نتائج الدراسة الحالية وجود انخفاض معنوي في الزيادة الوزنية لذكورالفئران المختبرية واناثها المعاملة بالجرعتين 6 و12 ملغم/ كغم من مبيد الدايمثويت مقارنة بمجموعة السيطرة. اوضحت النتائج عدم وجود فروق معنوية في مستوى انزيمات كبد ذكور الفئران المختبرية Aspartate transaminase (AST) وAlkaline phosphatase (ALP) المعاملة بالجرعتين6 و12 ملغم/ كغم, فيما انخفض معنويا مستوى انزيم Alanine transaminase (ALT) بالجرعة الواطئة 6 ملغم/ كغم فقط مقارنة بمجموعة السيطرة, في حين ارتفع معنويا مستوى انزيمات AST وALP لكبد اناث الفئران بالجرعتين6 و12 ملغم/ كغم فضلا عن ارتفاع مستوى انزيم ALT وبالجرعة الواطئة 6 ملغم/ كغم فقط مقارنة بمجموعة السيطرة. كما اشارت النتائج الى ارتفاع معنوي في مستوى يوريا وكرياتنين المصل لذكور الفئران وبالجرعة 12 ملغم/ كغم فقط, كما ارتفع معنويا مستوى اليوريا فقط لمصل اناث التجربة بالجرعة العالية 12 ملغم/ كغم فقط ولم تظهر النتائج اي فارق معنوي في تركيز كل من اليوريا والكرياتنين لمصل مجموعة الجرعة الواطئة 6 ملغم/ كغم مقارنة بمجموعة السيطرة. اظهرت نتائج فحص المقاطع النسجية لاعضاء الفئران المختبرية حدوث تغيرات نسجية مرضية في انسجة كبد وكلى ورئة وطحال وخصى ومبايض الفئران المحقونة بمبيد دايمثويت وبالجرعتين 6 و12 ملغم/ كغم واعتمدت شدة التغيرات على جرعة المبيد اذ ازدادت شدتها في الجرعة العالية 12 ملغم/ كغم, وتمثلت التغيرات في الكبد بحدوث تنكس Degeneration وتنخرNecrosis لبعض خلايا الكبد واحتقان دموي Congestion وتوسع Explansions في الوريد البابي والمركزي واشباه الجيوب الكبدية فضلا عن وجود نزف Hemorrhage في المساحة البابية وارتشاحInfiltration خلايا التهابية في مناطق متفرقة في الكبد, اظهرت التغيرات في الكلية تنكس بعض خلايا بطانة النبيبات في حين لوحظت خلايا اخرى متنخرة, اضافة الى انسلاخSloughing وحؤولMetaplasia بعض خلايا بطانة النبيبات بالاضافة الى احتقان ونزف وارتشاح خلايا التهابية في الكبيبات . اظهر الفحص النسجي لرئة الفئران المحقونة بجرعتي المبيد تغيرات نسجية مرضية متمثلة بوجود وذمات Odema وارتشاح خلايا التهابية بمناطق مختلفة ونزف واحتقان الاوعية الدموية, فضلا عن حدوث فرط تنسج Hyperplasia وانتفاخ Swelling الخلايا المبطنة للاسناخ الرئوية مما يسبب تضييق تجاويف تلك الاسناخ. اما في الطحال فقد تمثلت التغيرات النسجية بحدوث تضخم للعقيدات الطحالية وتوسع الاوعية الدموية وقلة في اعداد خلايا متعددة الانوية الكبيرة , فضلا عن حدوث زيادة في خلايا النسيج النخاعاني myloid tissue المكون للدم ووجود تكدس مواد غير حية شفافة materials Hayline بلون وردي في مناطق اللب الاحمر لاسيما حول العقيدات الطحالية . ادى الحقن بمبيد الدايمثويت الى حدوث تغيرات نسجية مرضية في انسجة خصى ومبايض الفئران المختبرية اذ تمثلت هذه التغيرات بحدوث تحلل كامل لمعظم الخلايا المكونة للحيامن في النبيبات المنوية مما يؤدي الى قلة اعدادها, كما لوحظ تسمك Thicking الغشاء القاعدي لبعض النبيبات المنوية فضلا عن احتقان الاوعية الدموية ووجود وذمات بين النبيبات, اما التغيرات النسجية التي حدثت في المبيض شملت حدوث فرط تنسج واحتقان الاوعية الدموية في برنكيما المبيض. | The physiological and histopathological changes were studied in some organs of laboratory mice injected with two doses of organophosphorus insecticide (Dimethoate). male mice were divided into three groups of eight animals per groups, and the same for females and male and female groups were injected as following : 1 - Control group : were intraperitonially (i.p) injected with 0.1 ml of normal saline.2 - Treated group 1 (T1) : were i.p injected with 0.1 ml of 6mg/kg of Dimethoate for a period of 30 days and represented as a low dose of the pesticide.3 - Treated group 2 (T2) : were i.p injected with 0.1 ml of 12 mg/kg of Dimethoate for a period of 30 days and represented as a high dose of the pesticide. The results of blood parameters in male mice showed a significant decrease in both of red blood corpuscle (RBC) count, hemoglobin Hb and Packed cell volume (PCV) of both doses (6,12mg/kg), and significant decrease in mean cell hemoglobin concentration (MCHC) in dose of 6mg/kg only as compared with control group. while in female, both of RBC count,PCV, mean cell hemoglobin(MCH) and MCHC were significantly decreased in both doses (6,12mg/kg), a significant decreased in Hb in dose of 6mg/kg in comparision with control group were recorded. The results of differential WBC counts of male mice showed significant decrease in total WBC counts in mice injected by both doses (6,12mg/kg),also the percentage of monocytes and granulocytes were significantly decreased in dose of 12mg/kg only as compared with control group, while the WBC count and the percentage of granulocytes were significantly decresed in female injected with 12mg/kg only of pesticide as compared with control group. In the male mice injected with two doses (6,12mg/kg). the results of current study explained decrease significantely in sperm count, and significant increases in percentage of malformation rate of sperm heads and tails , as well as, significant decreases in normal sperm count when compared with control group. The results of present study showed significant decrease in weight gain for male and female laboratory mice treated with two doses (6,12mg/kg) of dimethoate in comparision with the control group. The present data showed no significant differences in the level of liver enzymes Aspartate transaminase (AST) and Alkaline phosphatase (ALP) of male laboratory mice treated with two doses (6,12mg/kg), while significant decreased in the level of Alanine transaminase (ALT) enzyme with dose of 6 mg/kg only as compared with control was noticed, while significantely increased in levels of liver enzymes (AST and ALP) in female mice with two doses (6,12mg/kg), as well as,there was a significant increased in level of ALT enzyme with dose 6 mg/kg only when comparision with control. The level of urea and creatinine serum significantly increased whith two doses (6,12mg/kg), and there was no significant difference with dose of 6mg/kg when compared with control, also, the level of urea significantly increased in the serum of female mice with 12 mg/kg only, and there was no significant differences with 6 mg/kg as compared with control group. The results of histological section for some organs of laboratory mice showed occurrence of histopathological changes in the tissues of liver,kidney,lungs,spleen,testis and ovaries of mice injected with doses (6,12mg/kg), and The severity of the effects were dependent on dose, Histopathological findings of liver included degeneration and necrosis of some liver cells, blood congestion and explansions in the portal vein,central vein and hepatic sunsoids, as well as, the presence of hemorrhage in the portal area, infiltration of inflammatory cells in scattered area of it. In the kidney, histopathological changes showed degeneration of some tubular epithelium,while other cells where observed necrotic, sloughing and metaplasia of some tubular lining cells, as well as, congestion, hemorrhage and infiltration of inflammatory cells in the glumeruli. The histological examination of mice lungs were injected with two doses of dimethoate showed histopathological changes represented by the presence of edemas, infiltrations of inflammatory cells in different areas, hemorrhages and vascular congestion, as well as the occurrence of hyperplasia and swelling of cells lining of the pulmonary alveoli, causing narrowing cavities of those alveoli. In the spleen, the histological changes represented by occurrence of hypertrophy of the spleen nodules, expansion of blood vessels and decrease in the number of megalomultinucleated cells, as well as an increase in the cells of hematopoietic myloid tissue and presence of hyaline material, stained with pink color in the red pulp areas, especially around the spleen nodules. Injection with dimetoate caused histopathological changes in the testis and ovaries of laboratory mice represented by complete analysis of most spermatogenic cells in the seminiferous tubules,leading to decrease their numbers ,we also observed thickening of the basement membrane of some seminal tubule ,as well as vascular congestion and deposition of a live materials in interstitial spaces ,while the histological changes that have occurred in the ovaries included hyperplasia and vascular congestion in the stroma in addition to decrease number of ovarian follicles and corpora leutea.

واقع التلوث بالمركبات الهيدروكابونية في المياة والرواسب وبعض الاحياء المائية في مهر الكحلاء محافظة ميسان / العراق == The Status of Hydrocarbon Compounds Pollution of Water, Sediments and Some Aquatic Biota in Al - Kahlaa River - Missan Province /Iraq

Author name: صالح حسن جازع
Supervisor name: عبد الحسن يوسف العضب | حامج طالب السعد
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:
Abstract: The present study was performed to have knowledge of hydrocarbon compounds pollution status in the water (dissolved and particulate fractions),sediments ,two species of aquatic plants (Ceratophyllum demersum and Paspalum pespaioides) ,two species of fishes(Liza abu and Carassius auratus) and one species of molluscs (Pseudodantopsis euphraticus) .The samples were collected monthly for the period November 2012 to October 2013 from four different stations ) Al - Magideh, Treatment unit, Al - Husaichi and Al - Zubair) distributed along Al - Kahlaa River in Missan province , in addition to Reference station lies on the Tigris River before entering Amara district about 25 Km .The sampling's months were divided into four typical seasons although this is incompatible with Iraq climate.As well as measures of some ecological parameters (water and air temperatures ,pH ,turbidity, electrical conductivity ,dissolved oxygen , biological oxygen demand BOD5 and total suspended solid) which ranged between 22 and 42 C˚ , 18 and 31 C˚ , 6.9 and 7.82 , 42 and 172 NTU , 12 and 118 μS/cm , 4.66 and 9.25 mg/l , 1.46 and 5.04mg/l ,33 and 108 mg/l respectively .In addition to that the Organic Matter(OM) and the texture were measured in the sediment samples. OM values ranged between 0.078 and 0.927% , whereas the percentage of the texture was ( 15 %clay, 71% silt and 14% sand),(31 %clay, 63% silt and 6% sand), (33 %clay, 57% silt and 10% sand) , (40 %clay, 47% silt and 13% sand) and (18 %clay, 55% silt and 27% sand) in Al - Magideh, Treatment unit , Al - Husaichi , Al - Zubair and Reference stations respectively .The concentrations of total petroleum hydrocarbons (TPHs)were measured by using spectrofluorometer. Concentrations of total petroleum hydrocarbons in dissolved fraction ranged between 1.36 μg/l in Reference station and 13.87 μg/l in Treatment unit station during summer and winter respectively ,and in particulate fraction between 2.69 μg/g dry weight in Reference station and 29.92 μg/g dry weight in Treatment unit station during summer and winter respectively ,while in sediments they ranged between 3.16μg/g dry weight in Reference station and 135.18 μg/g dry weight in Treatment unit station during summer and winter respectively. Concentrations of total petroleum hydrocarbons in C. demersum ranged between 5 μg/g dry weight during winter and 58.97 μg/g dry weight during summer , while in P. pespaioides between 3.18 μg/g dry weight during winter and 43.44 μg/g dry weight during summer. Concentrations of total petroleum hydrocarbons in L. abu ranged between 4.81 μg/g dry weight during spring and 37.3 μg/g dry weight during summer ,while in C. auratus between 2.51 μg/g dry weight during spring and 29.303 μg/g dry weight during autumn . Concentrations ofbtotal petroleum hydrocarbons in P. euphraticus ranged between 5.98 μg/g dry weight during spring and 119.75 μg/g dry weight during summer .The concentrations of Polycyclic Aromatic Hydrocarbons(PAHs) and n - alkanes were determined in the laboratories of Nihran Omer (South Oil Company in Basrah province ) ,using Gas Chromatography.Total concentrations of n - alkanes in dissolved fraction ranged between 0.027μg/l in Reference station and 0.192 μg/l in Al - Husiachi station during winter and between 0.016 μg/l in Reference station and 0.039 μg/l in Treatment unit during summer ,and for particulate fraction between 0.108 μg/g dry weight in Reference station and 2.096 μg/g dry weight in Treatment unit station during winter and between 0.101 μg/g dry weight in Reference station and 0.160 μg/g dry weight in Treatment unit station during summer ,while in sediments they ranged between 4.491 μg/g dry weight in Reference station and 51.32 μg/g dry weight Treatment unit station during winter and between 2.31 μg/g dry weight in Reference station and 19.31 μg/g dry weight in Treatment unit station during summer.The range of carbon chain length of n - alkanes in dissolved fraction ranged from C8 to C34 and in particulate fraction from C15 to C34 distributed in two patterns ,the first pattern included compounds between C8 and C25 with the abundance of C19,C21,C23 and C25 odd carbon number of and C18,C20,C22 and C24 for even carbon number ,while the second pattern included compounds with the range from C26 to C35 predominated by C27 and C29 odd carbon number and C26,C28 and C30 even carbon number.In sediments they ranged from C14 to C35 and distributed in two patterns, the first pattern included compounds between C8 and C25 with the abundance of C17,C19 and C21 odd carbon number and C18,C20 and C22 for even carbon number ,while the second pattern included compounds with the range from C24 to C35 with predominance of C31and C33 odd carbon number and from C24 to C34 even carbon number.Total concentrations of n - alkanes in C. demersum ranged between 6.467 μg/g dry weight during spring and 59.483 μg/g dry weight during summer ,whereas in P. pespaioides between 34.7 μg/g dry weight during winter and 166.6 μg/g dry weight during summer .The distribution of n - alkanes compounds in plants chain length ranged from C15 to C35 with bimodal from C15 to C27and from C28 to C35 with predominance of C17 to C25 odd carbon number and C18 to C24 even carbon number in the first one ,whereas in the second the dominance compounds were from C29 toC33 for odd carbon number and from C26 to C32 even carbon number.cTotal concentrations of n - alkanes in muscles of L. abu ranged between 14.60 μg/g dry weight during winter and 73.15 μg/g dry weight during summer and C. auratus between 34.76 μg/g dry weight during winter and 166.61 μg/g dry weight during summer . The distribution of n - alkanes compounds in fish muscles chain length ranged from C16 to C35 with bimodal from C16 to C25 and C26 to C35 with predominance of C17 to C25 and C18 to C24 for the first one ,and from C27 to C33 and from C26 to C34 for the second. These reflected nutrition nature for these kinds of fishes which depend on algae ,phytoplankton ,zooplankton ,diatoms ,insects and crustacean . While in the mollusc (P. euphraticus) ranged between 95.16μg/g dry weight during spring and 220.36 μg/g dry weight during summer. The distribution of n - alkanes compounds in P. euphraticus chain length ranged from C14 to C35 with bimodal from C14 to C27 and from C28 to C35 with predominance of C17 to C21 and from C18 to C24 for the first one ,and from C29 to C33 and from C30 to C34 for the second.The values of Carbon Preference Index (CPI) and pristane to phytane ratio was calculated to know the hydrocarbon compounds source in water ,sediments and biota of Al - Kahlaa River ,since the CPI value ranged between 0.142 and 1.314 in dissolved fraction and between 0.045 and 2.193 in particulate fraction , 0.160 and 1.872 in sediments,1.075 and 8.638 in plants , 0.285 and 1.537 in fishes and between 0.354 and 4.457 in mollusc .Whereas the values of pristane to phytane ranged between not detected and 1.579 in dissolved fraction , not detected and 0.601 in particulate fraction , 0.447 and 3.008 in sediments, not detected and 0.821 in plants , not detected and 2.176 in fishes and between 0.822 and 1.347 in mollusc .These indicate that there are two sources of hydrocarbon compound in water ,sediments and biota ,biogenic from living organisms and anthropogenic from domestic ,industrial and petroleum pollutants except in plants .Total concentrations of PAHs in dissolved fraction varied from 0.739 ng/l in Reference station to1.974 ng/l in Treatment unit during winter ,and from 0.300 ng/l in Reference station to1.125 ng/l in Treatment unit during summer ,and in the particulate fraction they varied from 0.79 ng/g dry weight in Reference station to 24.42 ng/g dry weight in Treatment unit during winter ,and from 4.369 ng/g dry weight in Reference station to10.545 ng/g dry weight in Al - Husaich during summer ,while in sediments they varied from 4.906 ng/g dry weight in Reference station to 35.479 ng/g dry weight in Treatment unit during winter ,and from 2.391 ng/g dry weight in Reference station to 25.886 ng/g dry weight in Treatment unit during summer.It had been noticed that there were a predominance of high molecular weight PAHs on low molecular weight PAHs ,while Benzo(a)dAnthracene/(Benzo(a)Anthracene+ Chrysene) BaA/(BaA+Chr) ratio ranged from 0.218 to 0.804 in water and 0.520 to 0.66 in sediments. The Indeno (1,2,3 - cd)pyrene /(Indeno (1,2,3 - cd)pyrene + Benzo(ghi)perylene) InP/(InP+BghiP) ratio ranged between not detected and 0.578 in water and between not detected and 0.681 in sediments .The Fluoranthene/ Pyrene (Fl/Py)ratio ranged between 0.150 and 2.47 in water and between 0.10 and 8.490 in sediments ,this give an indication of the source of PAHs compounds in water and sediments which were mainly pyrogenic and few from them petrogenic .Total concentrations of PAHs in C. demersum ranged between 24.124 ng/g dry weight during winter and 87.289 ng/g dry weight during summer ,whereas in P. pespaioides between 1.616 ng/g dry weight durig winter and 16.661 ng/g dry weight during summer .The Lower Polycyclic Aromatic Hydrocarbons to Higher Polycyclic Aromatic Hydrocarbons ratio ranged between 0.006 and 0.009 in C. demersum and between 0.008 and 0.103 in P. pespaioides. TheBaA/(BaA+Chr)ratio ranged from 0.995 to 0.997 in C. demersum and 0.756 to 0.995 in P. pespaioides. The InP/(InP+BghiP) ratio ranged between 0.342 and 0.807 in C. demersum and between 0.19 and 0.645 in P. paspaloides . The Fl/Py ratio ranged between 0.096 and 0.988 in C. demersum and between 0.018 and 5.018 in P. pespaioides. These indicate that the sources of PAHs in these plants were both pyrogenic and petrogenic .Total concentrations of PAHs in muscles of L. abu ranged between 2.301 and 16.661 ng/g dry weight during winter and summer respectively and in C. auratus between 1.095 and 8.675 ng/g dry weight during winter and summer respectively ,whereas in mollusc (P. euphraticus) ranged between 1.86 and 6.88 ng/g dry weight during spring and summer respectively. According to ratios of Low Polycyclic Aromatic Hydrocarbons(LPAHs) to High Polycyclic Aromatic Hydrocarbons (HPAHs), BaA/(BaA+Chr), InP/(InP+BghiP) and Fl/Py ,they certainly reflected that the PAHs sources in fishes and mollusk are pyrogenic as a main sources and petrogenic as a small part .Also results of this study revealed the presence of seasonal and spatial variations in concentrations of TPHs ,n - alkanes and PAHs in water ,sediments and aquatic biota from Al - Kahlaa River ,the highest concentrations were recorded during winter whereas the lowest during summer ,also the highest values of these compounds were recorded in Treatment unit station in comparison with other stations.

تقييم تلوث الهواء باستعمال دليل نوعية الهواء AQI ودليل التحول APTI لبعض المواقع المختارة في محافظة البصرة == Assessment of air pollution using AQI and APTI indices in selected locations of Basra governorate

Author name: شيماء هشام شهاب الدين
Supervisor name: نايف محسن عزيز
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done for period from December to January 2015 including five station in three replicate for each station distributed in Basra Province (Al - Qurna district ,city center - Ashar ,Altenuma, and Zubair as well as region which is called Abu Al Khaseb , it is chosen as a control station for being agricultural area. The samples have been collected monthly for evaluating the parentage of gas pollution concentration (CO2,CO,NOx,SO2 and TSP) . The concentration of gas is measured by using gas analyzer device and the mount of particles in the air is measured by PDR - 1200. And black carbon ( BC) was measured by using Aethalometer . AQI was using for air quality for knowing the quality of surrounding air . Maps are drawn which show the spreading pollutants in Basra Province during the season by using GIS program . it is clear that there was differences concentration rates of pollutant gases in Basra province, the seasonally difference for gases pollutant proved that in Winter recorded high value for gases CO2, CO which reached 258.62 ppm for CO2 and 7.19 ppm for CO while in summer it is register the high value for NOx and SO2 which reached 0.406ppm for NOx and 0.319 for SO2 while TSP recorded difference reach high concentration 323.54 μg/m3 during Spring .While ,there is no difference has been recorded for black carbon in Autumn and Winter where as high concentration reached 25000 ng/m3 .The yearly difference in Al ashar shows that the highest for CO2 and CO during the period of studying followed by Al - Qurna recorded the highest concentration for NOx and SO2 while al - Zubair station recorded the highest concentration for TSP . Abu al Khaseb station recorded the lowest concentration for all gases and TSP because nature of the region which is formed from farms with lack of human activity .according to AQI which is use to measuringBthe air quality which shows the status of air and the degree of pollution , seasonally it is the highest rang for moderate in yellow color in Abu Khaseb station to unhealthy for sensitive groups in orange color in both al Zubair and Altenuma station where as al Qurna station recorded red color for unhealthy and in Al Ashar station they are record very unhealthy in purple color .while AQI for annually difference for the stations of study for unhealthy for sensitive groups in orange color for all stations except Abu Khaseb station , these groups are considered by negative affection human being health .Biochemical analysis has been done for leaves of plants that are spreading in the study area for knowing the effect of pollutants on biochemical indicators .The study included three kinds of plants which are phoenix dactylifera , ziziphus sp. And conocarpus lancifolius . Biochemical analysis test included total chlorophyll , water content, ascorbic acid and pH .the total chlorophyll estimated by using UV - spectrophotometer ,while ascorbic acid was estimated by using gas chromatography . The results shows significant different among leaves for different plants species in different study stations , the Date palm trees phoenix dactylifera recorded the highest concentration for total chlorophyll with the comparison with other plants. The seasonally differences show that lowest concentration of total chlorophyll in Winter reached 2.11 mg/g .The ascorbic acid recorded highest value in Winter reached 1.41 mg/ g and the water content rate of less recorded value in Spring reached %59.26 with no differences are recorded in the rest of the seasons . pH was recorded the highest rang in Winter reached 7.53.Ziziphus sp. recorded the lowest content of total chlorophyll for Winter reached 1.87 mg/ g and ascorbic acid recorded the highest value in Winter 1.31 mg/ g . whereas there was no significant different in water content in all seasons .pH recorded the high rang 6.64 in Winter , there were no significant in the rest of the season .Conocarpus lancifolius recorded the lowest value of total chlorophyll comparison with the other plants .while the lowest value for total chlorophyll recorded 1.42 mg/g in Winter . Ascorbic acid reached the highest concentration 1.31 mg/ g in Winter , while the water content recorded the lowest value in Winter 36 .88 % . While pH recorded 5.80 the lowest value in Winter.According to the biochemical analysis for plants for getting the value APTI for knowing the ability of tolerance of plants for air pollution which reflects the capability of the plants to tolerance of air pollution . The result of the study shows that Phoenix dactylifera recorded the highest value 7.36 in Al ashar station where as there are no significant recorded among the other station . Ziziphus sp. recorded the highest value 6.41 in al - Qurna station followed by al - Ashar station 6.07 whereas the other station did not recorded any significant . It has been noticed that Conocarpus lancifolius shows that lowest value of APTI which reached 3.91 in Al Zubair station and there were significant differences among the other stations.APTI was used for getting the value of Anticipated Pollution Index (API) which estimating biological characteristic and the economic value for plants. The results of API showed that the plants in Basra province Phoenix dactylifera recorded the value 6 and it was in excellent category. Ziziphus sp. recorded as poor plants recorded the value 2 . while Conocarpus lancifolius recorded the value 1 category as very poor plants.it has been noticed from values of APTI and API for phoenix dactylifera makes it typical plants as bioindicator and reducing the pollution .It is concluded for the current study that there are variation quantity and quality for gases and obvious spread for it is concentration of polluted gases and particles in air of the study stations in Basra. It was found that Phoenix dactylifera is a typical plants for using as bioindicator and reducing the air pollution .

عزل وتشخيص اميبا Acanthamoeba castellani ودراسة تاثيرها الممرض في الفئران المختبرية Mus musculus سلالة Balb/C == Isolation and Detection of Acanthamoeba castellani. and study their pathological effect on laboratory mice Mus musculus Balb \C strain

Author name: شيماء عبد الرزاق حميد
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: free_living species of amoeba belongs to the genus Acanthamoeba was recorded in the recent study , this species was classified as A. castellani was according to the morphological features , cultural properties , heat tolerance and osmosis .Astromoeba was also recorded that characterized by its star - shape and Naegleria spp. which isolated from environmental samples ,both are free living amoeba .Acanthamoeba was isolated from different environments , includings water and soil from various regions of Basrah Provinace and from gathered water in air - cooler basin and discarded water from air conditioning systems .It was isolated from the eyes of out - patients who were suffered from eye problemes and from healthy volunteers.Several culture media were used for parasite culturing including media S, Agar, PYG, Mix , Mud , Heat - killed E.coli coated agar ,Lock and Manitol media.The media differ in their capability for the parasite growth depending on their nutrients ingrediants and the suitable temperature for the growth ,and the high rate for the growth was on S medium 37◦C and there is increasing in the parasite size that it is a new medium and used for the first time .The PYG medium at 37◦C also cause a good growth for the parasite .The parasite susceptibility to Dexonium was examined in vitro which shows the activity of the drug against the parasite .The activity of other drugs including Cisplatin , Pentostam , Chloromphenicol , Methadin , Cifran and Genedin was examined in this study .Cifran and Cisplatin shows a close effect and less than others ,while Methadin does not affect on the parasite ,in other hand Chloramphenicol caused obvious malformation in the parasite and retardation in the numbers.The recent study showed the death dose for half of the lab mice infected with the parasite was 450 amoeba/ml.The study also proved that the parasite is one of the major causes for Blinding ,causes it invades the mice eyes and blinds them moreover it cause skin lesions , hypertrophy in spleen and kidneys and bleeding in brain and lungs .Different ways used in the infecting of mice by the parasite caused the death of them in two - four dayes ,The chronic infection cause death in long period.The parasite can infect the brain when it is injected in different ways through optic nerve ,peritonium ,veins , nose and skin ,in which it caused necrosis .The presence of the trophozoite and cyst cause WBC infeltration.The liver ,kidneys and lungs were also infected when the parasite injected through the optic nerve , peritonium ,veins ,nose and skin and caused necrosis , bleeding patches , gathering of the defence cells (WBC).

تقدير بعض العوامل البيئية والبكتريولوجية لمياه الصرف الصحي في محافظة البصرة / العراق == Assessment of some environmental and bacteriological factors of Wastewater in Basra Province, Iraq

Author name: سهى محمد علي محمد سعيد المظفر
Supervisor name: منال محمد اكبر | ميثم ايوب عبد القادر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير مياه الصرف الصحي على المحتوى البكتيري في محطة حمدان لمعالجة مياه الصرف الصحي /البصرة حيث تستلم المحطة مياه المجاري المنقولة عبر شبكة من الانابيب التي تستلم مياه المجاري من50% من مناطق مدينة البصرة. اختبرت ثلاث مواقع لاخذ العينات هي المحطة الاولى حوض التجميع الخارجي والمحطة الثانية حوض الترسيب الابتدائي اما المحطة الثالثة فهي المياه الخارجة من المحطة والتي تصب في شط البصرة.وشملت الدراسة ايضا قياس بعض الخصائص الفيزيائية والكيميائية لمياه المجاري وهي (الاس الهيدروجيني pH ودرجة الحرارة Temperature والملوحة Salinity والمتطلب الحيوي للاوكسجين BOD5 والفوسفات والمواد الصلبة العالقة الكلية TSS والمواد الصلبة الذائبة الكلية TDS والنتريت NO2 والنترات NO3) خلال الفترة من كانون الاول2014/ ولغاية ايار2015/.ويتضح من نتائج هذه الدراسة ان المتطلب الحيوي للاوكسجين BOD5 هو عامل مهم وله علاقة باعداد البكتريا الكلية الموجودة في مياه الصرف الصحي.كما شملت حساب كثافة المجاميع البكتيرية المتواجدة في مياه المحطات.ولحساب العدد الكلي للمستعمرات البكتيرية اخذت عينات مياه الصرف الصحي وزرعت على اوساط عامة وانتخابية وتفريقية مختلفة استخدم وسط الاكار المغذي Nutrient Agarكما واستخدم وسط المانيتول الملحي الانتخابي Mannitol Salt Agarلعزل المكورات العنقودية الذهبية Staphylococcusaureus ووسط اكار الماكونكي MacConkey Agar لعزل بكتريا القولون البرازية Escherichiacoli ووسط AgarXLD الانتخابي التفريقي (Xylose lysine deoxycholate Agar ) لعزل انواع البكتريا التابعة لجنس السالمونيلا Salmonella.تم ايضا دراسة حساسية العزلات تجاه المضادات الحيوية باستخدام طريقة الانتشار عبر الاقراص Disc Diffusion Test حسب طريقة كربي باور Kirby Bauer Method. ان دراسة حساسية عزلات E. coli توصلت الى ان العزلات البكتيرية ابدت نسب مقاومة عالية لاغلب المضادات الحيوية المختبرة في هذه الدراسة ، كذلك ابدت المكورات العنقودية الذهبية مقاومة عالية لاغلب المضادات الحيوية في حين كانت حساسة لكل من كلورامفينيكول (C) وسيبروفلوكساسين (CIP) ونورفلوكساسين (NOR) وكانامايسين (K). كانت عزلات السالمونيلا متباينة في حساسيتها تجاه المضادات الحيوية المختبرة في هذه الدراسة لكن ابدت عدم مقاومة او مقاومة واطئة للمضاد الحيوي CIP وNOR . ابدت المجاميع البكتيرية المختلفة قيد الدراسة انماط مقاومة مختلفة تجاه المضادات الحيوية اذ ابدت انماط مقاومة مختلفة. وكذلك ابدت العزلات البكتيرية المعزولة من مياه الصرف الصحي مستويات مختلفة من التحمل لتراكيز المعادن الثقيلة ،فيما يخص عزلات E. coliابدت العزلات مستوى عال من التحمل للنيكل بلغ 64% من العزلات كان MIC لها 4mg/Lوللكادميوم فان نسبة 93% من العزلات كان MICلها 0.8mg/L. اما بالنسبة للمكورات العنقودية الذهبية فان 89% من العزلات كان MIC لها 2mg/L لعنصر النيكلوبالنسبة للكادميوم فان 33% من العزلات كان MIC لها 0.4mg/L .وبالنسبة للسالمونيلا كان تحمل 100% من العزلات لعنصر النيكل هو عند 2mg/L في حين كان 50% من العزلات MIC لها 4mg/L كحد اقصى لعنصر الكادميوم. | The present study has been conducted to detect the effect of different parameters of sewage on microbial content at Hammdan for wastewater treatment in Basra city. The station receive sewage transported via net of pipes from different parts of Basra city .Three sites in the station for taking samples ,the first site is the external collection basin, the second site is primary sedimentation basin, the third site is water output that carry the water after treatment to flow at Shatt al Basra. Some characteristics of sewage were measured, these include pH, Temperature, Salinity, BOD5, TDS, TSS, Phosphate, NO2 and NO3 from period December/2014 to May/2015. In this study it is clear that BOD5 is an important factor related to total bacterial count in sewage. The bacteriological study of sewage has been done including measurement of total count of bacteria isolation and counting of Escherichia coli, Staphylococcus aureus and Salmonella spp. From different stations selective media is used for isolation including Nutrient Agar , MacConkey Agar , Mannitol salt Agar and XLD Agar . The antibiotic susceptibility of the isolates towards selected antibiotics have been studied using disc diffusion test. In the present study E. coli isolates showed high percentage of resistance to most of antibiotics. Staphylococcus aureus also showed high percentage resistance to most of antibiotics studied and found susceptibility to Chloramphenicol, Ciprofloxacin and Kanamycin. Variable suceptibility to tested antibiotics in the present study has been shown by Salmonella spp., Salmonella spp. were susceptible or show low resistance to Ciprofloxacin and Norfloxacin .Different Antibiotics Resistance Profiles has been shown by different bacteria groups studies. The bacterial isolates from sewage in this study have shown different levels of tolerance to different heavy metals (Nickel and Cadmium). E. coli tolerate high concentration for Nickel (%64) and for Cadmium (%93). High tolerance level for Cadmium has been shown for Salmonella spp (%50).

خصائص خمائر البيئة النهرية لمحافظة البصرة وقابلية بعضها على المعالجة الحيوية للعناصر الثقيلة والهيدروكربونات النفطية == Characterization of yeasts in river habitat in Basrah Province and an ability of some of them for Bioremediation of the heavy metals and oil hydro carbonate

Author name: سناء قاسم بدر
Supervisor name: باسل عبد الزهرة عباس
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

تقييم حالة النباتات المائية الغاطسة في بعض مناطق هور شرق الحمار وشط العرب جنوبي العراق باستخدام بعض دلائل التنوع الاحيائي == Assessment of the Status of Submerged Aquatic Plants in Some Areas of East Hammar Marsh and Shatt Al - Arab River Southern Iraq Using Some Biodiversity Indices

Author name: سما عباس عبد المجيد الزيدي
Supervisor name: دنيا علي حسين العباوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to assess the state of submerged aquatic plants in some areas of East Hammar marsh and Shatt al - Arab River, as measured by some of the chemical and physical properties of water and sediment in six stations, three of which are located in East Hammar marsh (Nagara, Sallal and Almshab) and the other three are located in the Shatt al - Arab (the island Muhammadiyah, Salhiya and Mhella). Environmental surveys of submerged aquatic plants were done and registered their presence, their vegetation cover and biomass as well as the biodiversity indices (diversity and evenness, and dominance) for 12 months from October 2015 until September 2016.The air temperature in the East Hammar marsh ranged between 19 - 45.3 C, water temperature 14.3 - 32.4C , transparency between 20 - 100cm , Turbidity between 20.9 - 65.3 NTU, depth of 50 - 160 cm and dissolved oxygen between 5.4 - 6.9 mg / L, pH between 7.6 - 8.6 , electrical conductivity between 3.8 - 12.5 mS / cm, nitrates between102.23 - 549.64 μg/L, effective phosphorus between 4.15 - 9.6 μg/L . As for the Shatt al - Arab River values ranged : air temperature between 18 - 48 C , water temperature was between 14.2 - 32.3 , Transparency between 23 - 90 cm, turbidity between 12.8 - 68.3 NTU , depth of 60 - 200 cm , dissolved oxygen between 5.4 - 7.9 mg / L , pH between 7.5 - 8.51 , electrical conductivity between 2.8 - 12.4 mS / cm , nitrates between 106.17 - 569.42 μg/L, effective phosphorus between 4.93 - 9.13 μg /L .BpH and electrical conductivity values of the sediments in the Shatt al - Arab and East Hammar marsh were reached 7.7 - 8.3 and 1.08 - 2.91 mS / cm and 7.5 - 8.4 and 1.12 to 3.05 mS / cm respectively, effective phosphorus values in sediment ranged in sediment of East Hammar marsh between 5.69 - 42.51μg/L, While in Shatt al - Arab fluctuated between 5.12 - 40.3 μg/L, and the value of the organic matter in the Shatt al - Arab were 5.6 to 14.5%, while in the East Hammar marsh reached 7.4 - 15%. The sediments are similar texture in the Shatt al - Arab and East Hammar marsh (Silty clay).The presence of three species were recorded in the Shatt al - Arab River included Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus, while five species in the East Hammar marsh Ceratophyllum demersum, Myriophyllun spicatum , Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus.As calculated biodiversity evidence was that East Hammar marsh was more diverse than the Shatt al - Arab, while dominancy was clear in the Shatt al - Arab, compared with East Hammar marsh.This study showed that the sudden rise in salinity values as well as increased nutrients led to degradation of water quality, which was reflected on the presence and vegetation cover of submerged aquatic plants as well as biodiversity resulting from the presence of Tilapia and other fish that use submerged aquatic plants as food as well as the human factor.

تاثير هرمون الثايروكسين على بعض المعايير الوظيفية للدم وبعض الجينات المنظمة لتمايز النسيج العظمي في الفئران المختبريةmusculus L. Mus. == Effect of Thyroxine hormone on some of physiological parameters of blood and some regular genes of bone differentiation in Laboratory Mice Mus musculus L.

Author name: سلمى سعيد عباس
Supervisor name: سامي جبر المالكي | علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير هرمون الثايروكسين على تمايز العظم وبعض المعايير الفسلجية والتي شملت المعايير الدموية والهرمونات المحرضة للقند واوزان واطوال الاجنة واوزان المواليد في الفئران المختبرية البيضاء Mus musculus L.,اذ قسمت اناث الفئران المختبرية في الدراسة الحالية على مجموعتين هما : المجموعة الاولى الرئيسية : لدراسة تاثير هرمون الثايروكسين L.Thyroxine hormone على المعايير الدموية للاناث الحوامل وغير الحوامل وعلى تمايز العظم في الاجنة(باستخدام طريقة تحضير المقاطع النسجية الاعتيادية للاجنة والطريقة الجزيئية باستخلاص المادة الوراثية (RNA) من الاجنة وباستخدام تقنية النسخ العكسي RT - PCR والبلمرة الكمية qRT - PCR ومعرفة تاثيره على اوزان المواليد والاجنة, وتالفت من 90 انثى بالغة بعمر(10 - 12)اسبوع وقسمت على مجموعتين ثانوية ,و هي مجموعة السيطرة والتي حقنت حيواناتها بـ 0.1 مل من المحلول الفسيولوجي0.9% ولمدة 10 ايام متتالية.بينما المجموعة المعاملة حقنت حيواناتها بـ0.1 مل من هرمون الثايروكسين وبجرعة مقدارها 0.5 ملغم /كغم/يوم ولمدة 10 ايام يوميا بعدها شرحت 5 اناث من كلا المجموعتين ,و زوجت 40 انثى المتبقية ولكلا المجموعتين مع الاستمرار بالحقن لحين تشريح الاناث واستخراج الاجنة خلال فترات عمرية متباينة (9و10و11و12و13و14و15) يوم.اما المجموعة الثانية الرئيسية خصصت لدراسة تاثير هرمون الثايروكسين على الهرمونات المحرضة للقند وهرمونات الدرقية وانزيم Alkaline phosphatase.وتتالف من 15 انثى وقسمت على ثلاث مجاميع ثانوية تضم كل منها (5) اناث, وهذه المجاميع هي : مجموعة السيطرة وحقنت بـ0.1 مل من المحلول الفسيولوجي 0.9%.ومجموعة المعاملة الاولى وحقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 15 يوم للاناث العذارى قبل التزاوج.ومجموعة المعاملة الثانية حقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 30 يوم . اظهرت نتائج الدراسة الحالية ان المعايير الدموية قد تباينت قيمها خلال الفترات الزمنية وللمجموعات ضمن المدى الطبيعي عدا في حالات قليلة ,اذ وجد هناك انخفاض معنوي في اعداد خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية واعداد كريات الدم الحمر,وكذلك قيم مستوى كمية الهيموكلوبينHb والنسب المئوية لحجم الدم المضغوط PCV(HCT)% ومعدل كمية الهيموكلوبين MCHومعدل تركيز الهيموكلوبينMCHC ومعدل حجم كريات الدم الحمرMCV والصفيحات الدموية PLT في فترات متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وبينت النتائج وجود زيادة معنوية في خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية, ومعدل تركيز الهيموكلوبينMCHC والنسبة المئوية لحجم الدم المضغوط %HCT ومعدل حجم كريات الدم الحمر MCVو الصفيحات الدميةPLT في مدد متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وسجلت نتائج الدراسة الحالية حصول ارتفاع معنوي في قيم هرموني T4,T3في الحيوانات المعاملة مع انخفاض معنوي في تركيز هرمون TSHفي الاناث المعاملة مقارنة مع السيطرة,و اوضحت النتائج ايضا انخفاضا معنويا في مستوى الهرمون اللوتيني LH وهرمون الحليب وللمجموعتين(15و30) يوم وزيادة معنوية في الهرمون المحفز للجريبات FSHللمجموعة الثانية(30 يوما) ,مقارنة مع مجموعة السيطرة, ولايوجد تاثير معنوي لهرمون الثايروكسين على هرمون الشحمون الخصوي ولنفس الفترتين مقارنة مع مجموعة السيطرة, وحصلت زيادة معنوية في مستوى انزيم الفوسفاتيز القاعدي بعد 30 يوما من الحقن بهرمون الثايروكسين مقارنة مع مجموعة السيطرة ,وحصول انخفاض في اوزان الاجنة بعمر 15يوما والمواليد للاناث المعاملة مقارنة مع مجموعة السيطرة , وبينت الدراسة زيادة معنوية في اطوال الاجنة المعاملة بعمر 9 و10و11و15يوما مقارنة مع مجموعة السيطرة. اما الدراسة النسجية فقد بينت ان الهيكل العظمي في الاجنة بعمر 9و10و11 يوما عبارة عن بداءات اعضاء تتكون من نسيج ميزنكيمي,ولم تسجل الدراسة النسجية وجود فرق بين مجموعة السيطرة والمعاملة في تمايز العظم .وظهرت بداية التعظم الغشائي والغضروفي في الاجنة بعمر12يوما ,اذ لوحظ بداية تمايز الخلايا الميزنكيمية الى ارومات عظمية,مع وجود القليل من المادة البينية الشبيهة بالعظم بين الارومات العظمية في منطقة الفك السفلي قرب غضروف ميكل ,والفك العلوي والعظم الحنكي واللامي وغضاريف الاضلاع ,اما في الاجنة بعمر13 و14و15يوما فقد ازدادت عمليات تمايز العظم بنوعية ,فاضافة الى النمو الذي حصل في العظام السابقة في تكوينها فقد ظهرت عظام جديدة في مناطق اخرى من هيكل الجنين تمثلت بعظام الصفيحة القاعدية والفخذ وحزام الكتف وحزام الحوض والاضلاع ,اما النتائج الجزيئية الخاصة بتمايز النسيج العظمي في اجنة الفئران المختبرية فقد اشارت الى التعبير الجيني لبعض جينات النسيج العظمي وتشمل Runx2وOSX وOsteo(OSC) وSialo(BSP) والتي بلغت ذروتها في مرحلة (13) يوم. ان لهرمون الثايروكسين تاثير على التعبير الجيني لبعض الجينات التي ترتبط مع التمايز النسجي للعظم اذ يؤدي هذا الى التغير في التعبير الجيني زيادة احيانا وانخفاضا خلال المدد العمرية المختلفة للاجنة .نستنتج من الدراسة الحالية ان زيادة مستوى هرمون الثايروكسين يسبب اضطراب في المعايير الدموية والتعبير الجيني لجينات العظم وانخفاض مستوى الهرمونات المحرضة للقند (LH وProlactin) وزيادة مستوى الهرمون المحفز للجريبات, وتعد هذه الدراسة الاولى على مستوى جامعة البصرة | the present study was performed to know the effect of thyroxine hormone on differentiation of bone tissue and some of physiological parameters, which included blood parameters and Gonadotropine hormones and Weights and Lengths and birth weight of mus musculus L.the females are divided into two groups ;the main first group which included 90 adult females (10 - 12)week, that subjected to study effect of thyroxine hormone on blood parameters of pregnant , nonpregnant females ,and bone differentiation of embryos (by using tissue sections method and molecular technique by RT PCR , and qRT PCR), and also known it is effect on weight of fetus and newborns; this group was also subdivided into two secondary subgroup : - control group ; was injected with 0.1 ml of normal saline (0.9%) for 10 consecutive days ,and treated group; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 10 consecutive days ,after that, 5 females from both groups were anatomized,while 40 females were mated of the both groups with continuous of injection until the females were subjected to anatomical practices and then the the embryo were taken during varied peroids (9,10,11,12,13,14,15) day. The main second group which included 15 females ,was selected to study the effect of thyroxine hormone on ;sexual hormone ,thyroid hormone and alkaline phosphatase . this group was divided into another 3 subgroups,one regarded as control group which ; was injected with 0.1 ml of normal saline (0.9%) for 15 consecutive days.and second the first treated group (T1) ; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 15 consecutive days ,and without mating of the females,and the second treated group ; (T2);was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 30 consecutive days and also without mating of the females. Results of the present study were shown that the blood parameters were varied in value between periods and groups (decreasing and increasing among normal value ) with exception in few cases , significant decrease of W.B.C , the granular cells ,monocytes, lymphocytes ,lymphocytes percentage ,granulocytes percentage and monocytes percentage during different periods of pregnancy in the treated animals compared with control , and was decreased RBC ,Hb ,HCT percentage ,MCH ,MHCH ,MCV, and PLT in varied periods , and we noticed a significant increase in WBC ,granular cells ,monocytes ,lymphocytes , granulocytes percentage , monocytes percentage , MCHC ,HCT percentage,MCV, and PLT in varied periods of pregnancy in the treated animals compared with control. Our study was exhibited significant increase in levels of T3 and T4 hormones of the treated animals compared with control, with significant decrease in TSH of treated animals , also significant decrease in LH and prolactin H and for groups(15 and 30 days) ,and significant increasing in FSH of second group (30 day) ,when compared with the control , and there was no effect of thyroxine on TEST H level and for 2 periods(15 and 30) , significant increasing in alkaline pho after 30 days from injection with TH H , decreased of body weight of embryos (15 days) and newborns of the treated females ,the current study showed significant increase of lengths of treated embryos (9,10,11,15 days). The histological study was explained the skeletal tissue ossification of embryos during (9,10,11)day post gestation was primitive organs of mesenchymal tissue and The histological study was not show asignificant differences of bone differentiation between treated and control groups,and intramemberaneous ossification was appeared of embryos at 12 days ,in this stage the begining of differentiation of mesenchyme cells was appeared into osteoblast , with a little of matrix like bone between osteoblasts in Mandible near the meckle cartilage , Maxilla, Palatine,and Hyoid, and endochondral ossification as in Ribs, where in embryos (13,14,and 15) The bone differentiation processes were increased of bone (the intramembranous ossification and endochondral ossification),in addition to the growth of previously formed bones,also new bones were appeared in different areas in embryo skeleton like Basal plate,femur,Scapula girdle , pelvis girdle and the Ribs . In other hand, the molecular results of bone differentiation in embryo ,were exhibited peak of expression of some of the genes of bone tissue (Runx2 ,OSX, Osteo(OSC) ,and Sialo(BSP) ) at 13 days of embryo age, also the thyroxine hormone was effected in expression of some the bone genes that included disturbanceas like increasing of expression during peroids and decreasing in other peroids of embryos life. We concluded from the present study that the increase of thyroxine hormone level caused disturbances of blood parameters and gene expression of the bone genes & decreasing of the level of LH & prolactin hormones and increase the level of FSH. This is the first study on the Basrah University

تقييم فعالية عقاري Nandrolone decanuate وAlbendazole للاصابات المختلفة بداء الاكياس العدرية في الفئران المختبرية سلالة Balb/c == Evaluate the effectiveness of a drug Nandrolone decanuate and Albendazole for injuries the different infections by hydatid cysts in mice Balb/c

Author name: سعيد فاضل مناتي الحمداني
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: تبنت الدراسة الحالية استعمال طرق مختلفة لاحداث الاصابة برؤيسات الاولية Protoscolex المستخرجة من الطور اليرقي ( الاكياس العدرية ) لطفيلي Echinococcus granulosus وهذه الطرق هي الحقن الوريدي والجنبي وتحت الجلد وكذلك عن طريق الغشاء البريتوني . اظهرت الدراسة حدوث الاصابة وتطور الاكياس العدرية في كل الطرق اعلاه . كذلك تم استعمال طريقة الحقن في اللفائفي والتجريع عن طريق الفم لكن لم تظهر الاصابة المستخدمة بهذه الطرق اي تطور للاكياس العدرية في حيواتات التجربة . كذلك حقن 650 رؤيس اولي في جميع طرق الحقن وتبين حدوث الاصابة في نفس مواقع الحقن بطريقة الحقن تحت الجلد والحقن الجنبي وعن طريق الغشاء البريتوني. ولكن طريقة الحقن الوريدي اظهرت حدوث الاصابة وتطور الاكياس العدرية في اماكن مختلفة من جسم الحيوان المختبري ويكون الكيس العدري متموضعا بين نسيج العضو المصاب في حين الحقن عن طريق الجنب وتحت الجلد والغشاء البريتوني يكون الاكياس العدرية المتطورة ملتصقة على سطح العضو المصاب . كذلك اثبتت الدراسة وجود اختلاف في الوان الاكياس العدرية المتكونه وحسب طريقة الحقن. استـــــعمل في هذه الدراســــــــة عقار Albendazole وعــــــقار Nandrolone decanuate كعقارات علاجات للاصابة المستعملة وتبين ان لها تاثير ادى الى موت الرؤيسات الاولية بتقدم فترة الاصابة واثبتت الدراسة ان عقار Nandrolone اكثر كفاءة من عقار البندازول وذلك من خلال الاختلافات في النسبة المئوية لموت الرؤيسات الاولية وكذلك بسبب هذا العقارشوه في شكل الرؤيسات وطريقة ابعاجها اذ كان الانبعاج الى الخارج في حين كان الانبعاج الى الداخل في الاصابات المعالجة بعقار البندازول . | The present study showed various methods for the infection by the protoscolex obtained from the larval phase of the Echinococcus granulosus . which involved the venus,pleural,subcutaneous and peritoneal injection . In all injection method 650 protoscolex were injected ,It was found that no infection happen in the same location to the injection which resulted by the subcutaneous,pleural and pretonal injections. On the contrary to thatresulted by venous injection in wich the injection occurred in varoes sites of the lab animals body ,and the hydatid cysts were pervasine with in the diseased infected organ tissues. Wile that resulted by pertonial ,subcutaneous and hered the surface of the organ. The colors of the hydrated cysts were also varied according to the infection methods.Albendazole and Nandrolone were used as treatments against the induced infections and it was found that Nandrolone was more efficient than albendazole,That was observed by the rates of the protoscolex caused by the action of the drugs and desmorphorphology and invagination outward while in albendazole was inward

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تشخيصية مظهرية وجزيئية للشريطيات المتطفلة على نورس مستدق المنقار Larus genei والكرسوع Himantopus himantopus شمال محافظة البصرة == Diagnostic, Morphological and Molecular Studies on some cestodes parasitized on Larus genei and Himantopus himantopus , North of Basrah province

Author name: سحر محمد علي محمد سعيد المظفر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During the period from August 2015 to July 2016, fifty three aquatic birds of Larus genei (slender - billed Gull) family Laridae and 52 of Himantopus himantopus family Recurvirostridae were collected from Al - masehab marsh north of Basrah Province.The current study was focused on these two birds due to its economic importance to the local people of the study area. These two birds were found infected with 10 species of cestodes. Echinocotyle longirostris and Infula burhini were recorded for the first time in Iraq. Larus genei was considered as a new host of Wardium fusa.The isolated cestodes were described and compared with other recorded species at different parts of Iraq and the world.The percentage and the mean intensity of infection of these 10 species of cestodes were counted monthly and seasonly together with single, double and trible infection during the whole year. The main percentage infection was reached to 95.2% and the main intensity was 13.49. In L. genei the percentage and mean intensity were 90.5% and 15.4 recepectively, while for H. himantopus were 100% and 11.8 recpectively. In L. genei the high percentage infection was found in August 2015 and September , November, and December 2016, and during March, April, May, June and July for H. himantopus. A significant difference was recorded between the percentage and the mean intensity of infection.Random Amplified Polymorphic DNA (RAPD) technique with random 6 primers was used to distinguish between Infula burhini and Acoleus vaginatus wich isolated from the bird H. himantopus due to the morphological similarities.The Scanning Electron Microscope (Pharmacy College, Basrah University) was used to photograph the scolex of A. vaginatus and I. burhini.

تشخيص وحياتية حشرة مع تصنيف الديدان الخيطية المتطفلة عليهافي محافظة ميسان == Diagnosis and life cycle insect Gryllotalpa gryllotalpa (L.) with a taxonomy parasitic nematodes in the province of Missan

Author name: سجى عماد نعيم الساعدي
Supervisor name: باسم هاشم عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: It was collected 318 mole cricket insects randomly from four regions in Missan including AL Kahala district by 191 insects, AL Mishraah by 62 insects, Kalat Salih by 61 insects and New Hay AL Hussein ( Centre of the city) by four insects. The period was from September 2013 to August 2014. It was diagnosed one species of mole crickets which is G. gryllotalpa decended from Gryllotalpidae families because of its essential charact ristics such as the general form of the insect, shape of the wings and legs, and its site and colour. In addition, there is a large lobe in the front legs of the insect and there one two species of veins in the front wings of the male used to attract females for mating. The life cycle of the insect was studied in the laboratory. Itstransformation is characterized by being imperfect progress, on egg, then a nymph and then an adult. The female lays about 100 eggs oval - shaped and grey in a room in the soil. The period of preegg - laying is approximately 100 days and the percentage of hatchling is 74% . The females remain alive after one day egg - laying is completed while the males remain 13 days after egg - laying. The eggs hatch into nymphs which pass stages of life development andit takes 115 day - Newly hatched nymphs are transparent and grey, its length is 10 mm with antennas and small cerci which are without wings. Wings begin to appear in the sixth stage of life. Then nymphs develop into brown adults with a large filled body. Its length is about 40 mm and all parts of the insect continues to grow in proportion to the size of the insect.After examining these insect, it showed that they are infected with seven species of nematode parasites. They are Binema ornate, Binema korsakowi, Binema anulinervus, Cameronia triovata, Cameronia multiovata, Mirzaiella asiatica, Grylophilla skrjabini. The Prevalence of those nematodes are 18%, 13%, 1%, 2%, 1.0%, 22% and 25.6% respectively. while the mean intensity of infection was 1.5, 1.6, 3.5, 4.5, 8, 1.5,2.15 respectively.Four of these species of nematodes were considered as new record in Iraq, These are : Binema anulinervus : Most important feature in the shape of the high - tail and long that look like a flagella.Mirzaiella asiatica : Featuring male this form of the parasite by the short and broad and meandering tail. The female is characterized by a length of the muscle of the esophagus and the existence of the tapes curled at his side. Cameronia triovata : The most important feature of this parasite egg shaped oval that is flat from one side. C. multiovata : What also distinguishes this form of the parasite is a large and long oval egg.Nematodes were a taxonomically quoited and illustrated.The species B. ornate and G. skrjabini appear repeatedly over 10 months followed by the parasite B. korsakowi for eight months then followed by M. asiatica for seven months, as for the parasites B. anulinervus, C. triovata, C. multiovata for two months

دراسة مظهرية وجزيئية بتقنية RAPD لبعض الاصناف الغيبانية لنخيل التمر في البصرة == Morphological and Molecular Study by RAPD Technique for Some Un Known (Ghaibany)Cultivars date - palme In Basrah

Author name: زينب شعلان عبد الرزاق علي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية اثناء الموسم الزراعي للعام 2015م على احد عشر صنفا بذريا (غيباني) لنخيل التمر Phoenix dactylifera L. النامية في البصرة وهي اصناف جديدة تسجل لاول مرة, درست ثمان وعشرون صفة من صفات النمو الخضري, وست وعشرون صفة من الصفات الثمرية كما. درست صفات البذور, فتبين ان الاصناف المدروسة اختلفت في الخواص الخضرية والثمرية, اذ اظهرت الدراسة ان كلا الصنفين نبوي والصنف نجمة تميزا بشكل التاج الشبه منفتح في حين كانت الاصناف الباقية مضمومة التاج. وكذلك انفرد الصنف نجمة عن باقي الاصناف بلون الاوراق الاخضر الشمعي. وتراوح معدل طول الورقة من 2.68 م في صنف الوردية الى 4.35م في الصنف نمروشة, كما تراوح معدل طول منطقة الاشواك من 56 سم في صنف دكلة عبد الى 160سم في الصنف ام اللسين. وتباينت الاصناف في معدل عدد الاشواك فكان ما بين 18 شوكة في الصنف وردية الى 47 شوكة في الصنف ام اللسين. واختلفت الاصناف بنمط ترتيب الاشواك على المحور الوسطي فقد انفرد الصنف برماني بنمط توزيع الاشواك الثنائي وامتاز الصنف وردية بالنمط المفرد الثنائي في حين كان النمط المفرد هو السائد في الاصناف الباقية, واتضح من الدراسة ان النسبة المئوية لطول منطقة الاشواك الى طول الورقة الكلي هي من الصفات التي تتسم بالثبات دونا عن باقي الصفات الخضرية. اما بالنسبة للخواص الثمرية فقد تراوح معدل طول العرجون للاصناف المدروسة مابين 66 سم في الصنف رحماني الى 152سم في الصنف برماني والذي يعد من العراجين الطويلة, وانفرد الصنف دكلة داود بلون العرجون الاصفر المائل للوردي اما الصنفين برماني ونجمة فقد كان لون العراجين فيهما اخضر في حين كان لون العراجين في الصنفين وردية ونبوي اصفر يميل للاخضرار واصفر في باقي الاصناف. اما الخواص الفيزيائية المدروسة للثمار فقد سجل الصنف دكل داود اقل المعدلات في حجم الثمرة في مرحلتي الخلال والتمر والتي كانت 5سم3 و2.6سم3 على التوالي, وامتاز الصنف الزريجي بكبر حجم ثماره لكلا المرحلتين الخلال والتمر 17سم3 و10.24سم3 على التوالي, وتبين ان طعم الخلال في الاصناف وردية ودكلة عبد والصنف رحماني والصنف زريجي عفصي اما الاصناف الباقية فهي حلوة المذاق. وامتاز الصنف نجمة بكونه مبكر جدا في موعد النضج وكانت الاصناف الباقية مبكرة النضج في حين كان الصنف برماني متوسط النضج, وامتازت اصناف الدراسة بكون التمور فيها طرية القوام عدا الصنف رحماني الذي كان قوام التمر فيه شبه جاف. واستخدمت الدراسة برنامج التحليل الالي لتحليل المكونات الرئيسة للثمار في مرحلة الخلال, فتبين ان الاصناف انقسمت بمجموعتين وحسب التقارب في البيانات المغذاة للبرنامج فانعزل الصنف الزريجي بمجموعة مستقلة لكون بياناته متفردة, اما المجموعة الثانية فقد توزعت الاصناف فيها الى تحت مجموعة, صغرى ضمت الاصناف دكلة داود ونجمة واقترب منهما الصنف وردية, اما الاصناف الباقية فتوزعت هي الاخرى بتحت مجموعتين. استخدمت الدراسة الجزيئية تقانة PCR RAPD لتثبيت البصمة الوراثية للاصناف المدروسة ولكشف الارتباط الوراثي بينها, وجرى باختبار عشرة بادئات عشوائية من مجموعة OPA وبادئان من مجموعة OPB. تم تحليل نواتج التفاعلات للبادئات مع Genomic DNA الاصناف كافة وكل على حده لتحديد مدى كفات البادئ فكانت النتيجة ان اربعة من البادئات ( OPA0.2 وOPA0.3 وOPA0.5 وOPA0.6) نجحت في كشف التباين بين الاصناف المدروسة, وبينت نتائج التحليل العنقودي للنواتج التفاعلات للبادئات ان الاصناف توزعت بمجموعتين, صغرى ضمت كل من الاصناف زريجي ونمروشة والصنف ام اللسين ومجموعة كبرى ضمت الاصناف الباقية, وقد توزعت فيها الاصناف الى تحت مجموعة ثانوية. مما يشير الى الاصول المشتركة, وبينت الدراسة امكانية انتخاب اصناف بذرية تمتاز بخصائص ثمرية جيدة وتكثيرها لدعم الاقتصاد الوطني. | This study was performed during agronomic season in 2015 on 11 cultivars (ghaibany) Phoenix dactylifera L. that grow in basrah, and these cultivars are new recorder. Twenty eight characteristics of vegetative growth were studied, seed characteristics were studied also. It was concluded that the studied cultivars varied in there vegetative and fruit qualities. The study showed that both cv Nabawy and cv Najma distinguished by semi - opened crown but the other cultivars had closed crown . In addition, cv Najma marked by waxen green leaves. The rate of leaf length ranged from 2.68 m in. cv Wardiah to 4.35m in cv Nammrosha. besides the length of spine area ranged from 56 cm in cv Deglat Abd cultivars to 160 cm in cv Am - Ellsian. The cultivars also varied in the rate of spine number which was 18 in cv Wardiah an 47 in cv Am - Ellsian. The cultivars differed in the style of spine arrangement on rachis since cv Bermany distinguished by the dual style of spine arrangement, while in cv Wardiah marked by single dual style, however the single style was prevalent in other cultivars. The study clarified that the percentage between spine area to the total length of the leaf was one of qualities that characterized by stability that differed from other vegetative characteristics. As for fruit characteristics, the length of peduncle of cultivars concerned ranged from 66 in cv Rahmany cultivars to 152 in Bermany and that was regarded long peduncle. cv Deglat Dawood marked by colour of peduncle which was yellow look like rosy, whereas cv Bermany and cv Najma the colour of their peduncle were yellow look like green and yellow in the rest. As for physical characteristics of studied fruit, cv Deglat Dawood recorded the lowest rates in the volume of fruit during the phases of khalal and dates, the volume was 5 c.m and 2.6 c.m respectively, cv Zraigai distinguished by the bigness of the volume of the fruit for both phases of khalal and date 17c.m and 10.24 c.m. The taste of khalal in the cultivars of cv Deglat Abd, cv Wardiah, Rahmany, cv Zreigai was tannic while the taste of other cultivars were sweet. cv Najma cultivars distinguished by being so early in maturity, the other cultivars were early in maturity but cv Bermany was middle in maturity. The dates of studied cultivars were soft except cv Rahmany that was semi - dry. The study used a program of automatic analysis to analyze the main components of fruit in khalal phases the, it became clear that the cultivars divided into two groups according to the convergence data that fed to the program. cv Zraigai cultivars was isolated in independent group because its data were unique, but the second group ,cultivars in which were classified in small group included cultivars of cv Deglat Dawood and cv Najma and cv Wardiah approached to this group. The other cultivars classified into two groups.The molecular study used P C R RAPD technique to fix genetic print of studied cultivars and to discover the genetic link among them. Ten of random primers of OPA group were tested and two of OPB were tested too. The products of reaction of primers were analyzed Genomic DNA for cultivars totally and individually; to determine the efficiency of primer the results was that for of primers succeeded in discovering the variation among cultivars concerned. The result of cluster analysis of reaction products of primers showed that the cultivars divided into two groups : small group included cv Zairgai, cv Nammrosha and cv Am - Ellsian cultivars, and big group contained the other cultivars in which cultivars distributed into secondary group and that refer to the common origin. The study showed the ability of electing and increasing Ghaibany cultivars that marked by good characteristics to support national economy

دراسة تصنيفية وحياتية للفطريات الناقصة الملونة في البصرة == Taxonomical and Biological study of Dematiaceous imperfect - fungi in Basrah

Author name: زينب خلف عبد الله
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During this study 23 species belong to 10 genera of imperfect fungi were isolated from different natural sources ( plants, soil , and ari) from Basrah govermanete using different isolation methods.Among the isolated fungi the genus Alternaria was selected to identify its species by using the morphological characteristis by light microscopy and Scaning Electron in Microscope (SEM) and verified by molecular and chemical methods. Eight species of Alternaria were identified namely; A.alternata (isolate1 and 2) A.chlamydospora A.citri, A.raphani , A.longipes , A.infectoria A.tenuissima and A.chrysanthemi based on light microscope examination.Taxonomic relegation of these species were confirmed by SEM image. SEM revealed that there was differences in the conidial wall ornamentation .The molecular analysis using PCR depending on the loci ITS1, and ITS4 700bp which confirmed the morphological identification of most of species namely A.alternata (isolate 1 and 2) , A.chlamydospora , A.longipes , A.infectroia and A.tenuissima. However , A.citri was related to A.alternata, A.raphani was belonged to A.infectoria and A.chrysanthemi is belonging to A.lolii. Nevertheless ,by using the loci OPA1 - 3 there was no variation among the species of Alternaria ,this is may be due to the inadequate method applied.The genetic variation of 36 isolates of A.alternata was studied using 9 - primers by RAPD - PCR technique. All primes gave an amplification of DNA for all the fungal isolates. Among the primers OPC08 exhibited a highest efficacy reaching 235 total bands .In the present study the antibacterial activity of fungal crude extract was tested. It appeared that the extract exhibit an antibacterial bioactivity against E.coli , S.aureus , S.epidermidis and S.saprophyticus.However, the extract of A.alternata (isolate 1 and 2) showed an inhibition growth against all the tested bacterial strains. This study was also revealed that the fungal filtrates of Alternaria species possessed various chemical compounds. However, there were differences in the chemical composition among the fungal filtrates.The results showed that Alternaria species ( A.alternata isolate 1 and 2, A.chlamydospora A.citri, A.raphani , A.longipes) were capable to biosynthesize silver nanoparticles, when the fungal filtrate were treated with AgNO3 solution after72 hrs incubation .Silver nanoparticles synthesis was checked by the change of colour from yellow to brown and confirmed by Uv - vis spectrophotometry at wave length (420, 440 nm). SEM image showed that the silver nanoparticles with spherical shape and size ranged between (20 - 72nm). Aconclusion can be derived that application of SEM,chemical analysis of fungi extracts ,molecular analysis and the silver nanoparticles characteristics are useful in the taxonomy of fungi to solve the overlapping of the morphological identification .Taxonomic key were prposed for the species of the genes Alternaria based on the morphology , chemical compound with the fungal extracts and size of silver nanoparticles synthesized by fungi .Melanin pigment identify by TEM microscope and it appeare like dark drop inside the cell .

دراسة المايكوبلازما المصاحبة لحالات العقم في الرجال في محافظة البصرة == Study of Mycoplasma associated with men infertility in Basrah province

Author name: زهراء كاتب جمعة
Supervisor name: غيداء جاسم عبد النبي الغزاوي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed current was to determine the role of Mycoplasma in men infertility in Basrah province . A100 seminal fluid specimens were collected from men who were admitted to infertility center in Basrah province from 1/10/2015 to 31/5/2016 .Besides 50 semen specimens were collected from those who determine fertility men as control group. Samples then were cultured on Monophasic - Diphasic - Culture - Setup(MDCS) to isolation Mycoplasma then the samples were cultured on Blood agar, MacConkey agar and Chocolate agar to isolation bacteria other than Mycoplasma .The isolated Mycoplasma from 48 cases were concluded five species : Ureaplasma urealyticum 38% ; Mycoplasma hominis 21%;Mycoplasma genitalium 15% ; Mycoplasma pirum 15% and Mycoplasma fermentans 11% . Where the last two species are first recorded in Iraq from semen.This Study also recorded that the patients of 30 - 39 years old were of high infertility rate and Mycoplasma infection percentage.High the percentage of a single infection was related to U. urealyticum and Mycoplasma hominis .By using the double Vitek 2 system 22 other bacterial species were diagnosed from seminal fluid. some of these species were first recorded in Iraq and represented types next : Staphylococcus haemolyticus ,,Enterococcus faecalis Staphylococcus hominis , Staphylococcus warneri ,Kocuria rosea , Streptococcus pneumoniae , Kocuria kristinae ,Granulicatella elegans , Vibrio cholera , Aeromonas salmonicida ,Enterococcus faecium , Morganella morganii, Enterobacter cloacae ,Alcaligenes faecalis , Staphylococcus xylosus , Micrococcus lutes ,Granulicatella adiacens, Kocuria varians,And the most exhibited and isolated bacterial species was of Staphylococcus haemolyticus.Mycoplasma infection were noticed in patients who were suffering from other diseases like varicose 18.75% and diabetes 8.33% and bladder nerve pressure 4.16% .In this study the primary infertility was 82% included 39 cases infected with Mycoplasma , while for the secondary infertility 18% nine cases were infected with Mycoplasma .High infection rate of Mycoplasma 69.23% was recorded in patients who underwent a varicose other Surgery compare with the other patients.Mycoplasma infection for the five species was clear quantity and quality in semen analysis for infertile men was less than control group .Where the semen volume was very small and the sperms count was effect on also the sperm morphology and activity (mobility) were affected by Mycoplasma infection . Pus cells and RBC. Were appeared in semen.This study also , confirmed that there is a 7% of semen from infertile men , was free from bacterial infection.

عزل وتشخيص بعض انواع من بكتريا المايكوبلازما من عينات سريرية في مدينة البصرة == Isolation and identification of some Mycoplasma species from clinical samples in Basrah city

Author name: رواء صادق مجيد
Supervisor name: غيداء جاسم عبد النبي الغزاوي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study , a total of 150 clinical samples were collected (50 samples of sputum ,50 swabs of gingiva and 50 vaginal swabs ) from patients visited Basrah general hospital and from the 1st specialized center of dental medicine in the city of Basrah . This study extended from January 2015 to May 2015. Samples were taken from males and females (except vaginal swabs where were collected from females only) .Their ages ranged from 6 - 70 years old. These samples were collected and cultured in a method monophasic - diphasic culture setup ( MDCS ) . Three types of Mycoplasma were isolated : Mycoplasma pneumoniae from sputum , Mycoplasma salivarium from gingival swabs and Ureaplasma urealyticum from vaginal swabs .These isolates diagnosed by biochemical tests and PCR. Mycoplasma spp. were isolated from 76 individuals out of 150 enrolled in this study, infection rate was 50.5% , 25 cases were diagnosed as M. pneumoniae (50%) , 13 cases were diagnosed as M. salivarium (26%) and 38 cases were diagnosed as U. urealyticum (76%).Mycoplasma was studied in related with two factors : sex and age . In this study, the Mycoplasma medium is modified , egg yolk was used instead of horse serum as source of cholesterol and also used two amino acids Arginine and Cysteine as well as urea ( when diagnosing U. urealyticum ) to support Mycoplasma growth. Besides that , thallium acetate was replaced by sodium benzoate , finally , magnesium sulfate was used as a sign of ammonia when diagnosing U. urealyticum.In this study , PCR technique used to diagnose species of Mycoplasma using ATPase gene ,16s rRNA gene and Urease gene .Sixty - eight isolates were identified by PCR technique , 25 of them were M. pneumoniae , 13 were M. salivarium and 32 were U. urealyticum.

دراسة مجتمعات اللافقريات القاعية الكبيرة في ثلاث بيئات مائية مختلفة في جنوب العراق == Synecology of Macrobenthic Invertebrates of Three Different Aquatic Habitat at Southern Iraq

Author name: رغد زيدان خلف
Supervisor name: هيفاء علي حمزة | مرتضى يوسف العباد
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study aimed macrobenthic invertebrats communites in three different aquatic environments southern Iraq. Eight stations were chosen for this purpose, Four of them distinction by being with running water and affected with the tide of Arabian Gulf,these include two stations on each of Shatt Al - Arab River (Al - Mohamadiat and Al - Salihia) and east part of Al - Hammar marsh (Al - Burgah and multaqaa Al - Mashab and Al - Sallal).the other four stations were not affected with the tide and located on the central marshes, two stations on each of Aum Al - Showaich marsh (Al - Sibitia and Al - Badria) and Al - Chebaeish marsh (Abu Sobat and Al - Sihaqee).The samples of water and sediment were collected monthly during the period from April 2014 to March 2015.Some environmental factors for each station were measured these include air and water temperatures, pH ,electrical conductivity, turbidity, dissolved oxygen, biological oxygen demand, total dissolved soild, total hardness, Calcium, nitrate , reactive phosphate, sulphate, total organic carbon and soil texture. The macrobenthic invertebrates samples which collected by Three replicates monthly for each station were isolated and identified,then densities and relative abundance were calculated.Some ecological indices such as diversity, richness, evenness, dominance, beta diversity and similarity indices(qualitative and numerical) were studied. Canonical correspondence analysis was adopted to evaluate the effect of the measured environmental factors on the occurrence and density of the studied macrobenthic invertebrates.The results showed that the variables of the stations were ranged as below : Air temperature (12 - 44)C̊ ,while the water temperatures (10.6 - 35)C̊ , PH (7.5 - 9.1), EC (2.07 - 13.7) mS /cm, Turbidity (4.2 - 199) NTU, DO (5 - 11.5) mg/l , BOD5 (0.3 - 3.5)mg/l , TDS(1245 - 10220)mg/l , TH (531 - 2928) mg/l , Ca+2 (80.2 - 320)mg/l , NO3 - (0.26 - 15.25) mg/l , PO4 - 3 (0.032 - 2.9) mg/l , SO4 - 2 (250 - 910)mg/l , and for sediments : TOC (9.3 - 22.5)% and soil texture ranged between sandy silt to muddy silt.The total number of recorded taxes were 80,representing by 16 species belonging to Annalida, 36 genus to the Aquatic insects (28 genus of them were first record in the middle wetlands), 16 species to Crustacea and 12 species to Mullosca. The species Pomacea canaliculata was recorded for the first time from Shatt Al - Arab river. The recorded taxes were classified according to its occurrence periods into four classes : dominant, fluctuated, less abundant and rare.The monthly density of each taxa of macrobenthic invertebrate and for each main macrobenthic invertebrate group in all stations was calculated. The results revealed that the densities were ranged from( 0 - 7115, 0 - 485 , 0 - 703 and 0 - 1199) ind/m2 for the groups of annelid, aquatic insect, crustacean and mollusca respectively. While the total density of all macrobenthic invertebrate groups was ranged between( 0 - 7367) ind/m2.The results of annual relative abundance for each main macrobenthic invertebrate group in all study stations referred that the value 66.3% was recorded for the annelids, while the lowest one 6.1% was recorded for the aquatic insects. On the other hand, the annual relative abundance for each main group in each station was investigated, and was ranged between (0.9 - 68)% recorded in Shatt Al - Arab river stations for the groups of aquatic insects and annelids respectively,( 0.4 - 67)% recorded in east Hammar marsh stations for groups of water insects and mollusks respectively, and (1 - 82)% recorded in Middle marshes stations for the annelids. The annual relative abundance for total macrobenthic invertebrate group in all stations was ranged from (2 - 27)% recorded in Al - Sibitia and Al - Sihaqee stations respectively. The annual relative abundance for the species and genus which recorded during our study revealedthat all highest values (94, 96.58 and 100)% were recorded for the species Limnodrilus hoffmeisteri in stations of Shatt Al - Arab River, east Hammar marsh, and Middle marshes respectively.The monthly relative abundance for main macrobenthic invertebrate groups in all station was studied. The highest value which recorded from Shatt Al - Arab river stations (Al - Salihia station) was reached 95.8% and recorded for the annelids, while reached 100% either in east Hammar marsh stations(Al - Burgah) for the mollusks and in Middle marshes stations (Al - Sibitia) for the crustaceans.Some ecological indices for each for each main macrobenthic invertebrate group from all stations totally were calculated. About the spatial diversity the range of Shannon - Wiener diversity index were between 0 - 0.6674 for annelids, 0.8718 - 1.92 for aquatic insects,0.5404 - 1.917 for crustaceans and 0.3035 - 1.15 for mollusks, while were 1.076 - 2.085 for all the benthic groups totally.The values of Richness index were from 0 - 1.127 for annelids,0.778 - 3.683 for aquatic insects, 0.5212 - 2.104 for crustaceans and 0.2543 - 1.277 for mollusks, while were 2.321 - 5.225 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 0.9629 for annelids,0.4649 - 0.7419 for aquatic insects ,0.3016 - 0.8327 for crustaceans, and were 0.2189 - 0.8974 for mollusks, while were 0.3121 - 0.77 for all benthic groups totally. The values of Dominance index were ranged from 0.6129 - 1 for annelids, 0.343 - 0.7736 for aquatic insects ,0.2991 - 0.8772 for crustaceans,0.5075 - 0.9845 for mollusks while were 0.2638 - 0.7762 for all benthic groups totally.About the temporal diversity the values of Shannon - Wiener diversity index were between 0 - 0.99 for annelids, 0 - 2.01 for aquatic insects, 0 - 1.5 for crustaceans and 0 - 1.637 for mollusks, while were 0 - 1.981 for all benthic groups totally. The values of Richness index were from 0 - 0.969 for annelids, 0 - 2.717 for aquatic insects, 0 - 1.314 for crustaceans and 0 - 731 for mollusks, whilewere 0 - 3.047 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 1 for each of annelids, aquatic insects and crustaceans, and were 0 - 0.992 for mollusks, while were 0 - 971 for all benthic groups totally. The values of Dominance index were ranged from 0 - 1 for each of four benthic groups, while were 0.3 - 1 for all benthic groups totally.Whittaker Beta index between the three different aquatic habitats for each main macrobenthic invertebrate group was calculated. The lowest value 1.14 was recorded for the mollusks between the middle marsh of um Al - Shoowaich and Al - Chebaeish, and highest value 1.66 was recorded for the aquatic insects between the east Hammar marsh and marsh of Al - Chebaeish, while the index values for all groups of macrobenthic invertebrate totally were ranged from 1.33 between the Shatt Al - Arab river and east Hammar marsh to 1.54 between the Shatt Al - Arab river and marsh of Al - Chebaeish.Jaccard Similarity index for each main macrobenthic invertebrate group for each stations was calculated.The values of index for annelids were ranged from( 0 - 89)% the lowest value recorded between Al - Burgah and Al - Sibitia stations and the highest value between Al - Salihia and Multaqaa of Al - Mashab and Al - Sallal stations, for the aquatic insects the values of index between (8.3 - 46.7)% the lowest value between Al - Salihia and Al - Sibitia and the highest value between Al - Mohammediat and Al - Badria stations, for the crustaeceans values ranged(14.3 - 77.8)% the lowest value between Al - Mohammediat and Al - Badria stations while the highest value between Al - Sibitia and Al - Badria,and for mollusks were between(18 - 80)% the lowest value between Al - Mohammediat and Al - Burgah stations while the highest value between Multaqaa of Al - Mashab and Al - Sallal and Abo Sobat stations.The index for all macrobenthic invertebrate groups totally also calculated and its values were from (15 - 58.5)% recorded between Al - Mohammediat with Al - Sibitia stations and between Al - Mohammediat with Al - Salihia stations respectively.Bray - Curtis index was calculated monthly for each main macrobenthic invertebrate group in each stations .Also the Bray - Curtis index was calculated annually between stations and the results refer that the highest values as the following : 0.993 for the annelids between Al - Badria and Al - Sihaqee stations, 1 for the crustaceans between Al - Salihia and Al - Sibitia stations, and 0.96 for the mollusks between Al - Sibitia and Abo Sobat stations.The highest value for all benthic group totally was 0.983 between Al - Salihia and Abo Sobat stations.On other hand Bray - Curtis index was calculated for all macrobenthic invertebrate groups totally between the three aquatic habitats and the values were ranged from 0.5 recorded between the wetlands of um Al - Shoowaich and Al - Chebaeish to 0.875 recorded between the east Hammar marsh and wetlands of um Al - Shoowaich.The Canonical Correspondence Analysis (CCA) by using Canoco application revealed the clear effect of different ecological factors on numbers of the individuals and species of each group of macrobenthic invertebrate in all stations during the study period.

تاثير المركبات الثانوية لاوراق نبات كف مريم Vitex agnus - castus L.مع مبيد بولو500 في بعض جوانب الاداء الحيوي لذبابة التبغ البيضاءBemisia tabaci (Genn.) (Hemiptera : Homoptera : Aleyrodidae) == Effect Of secondary Plant compounds of Vitex agnus - castus L. With PoLo500 Insecticide in Some Biological aspects of Tabacoo Whitefly Bemisia tabaci (Genn.) (Hemiptera : Homoptera : Aleyrodidae

Author name: رشا حطاب عبد الله الاسدي
Supervisor name: ناصر عبد علي المنصور
General topic: Biology
Specific topic: Zoology - Immunology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لتقييم كفاءة المستخلصات النباتية والمركبات الفعالة لاوراق نبات كف مريمL. Vitex agnus - castus في الاداء الحيوي لذبابة التبغ البيضاء Bemisia tabaci (Genn.) من خلال حساب معدلات الهلاك عند كل معاملة وبتراكيز وفترات زمنية مختلفة من دور البيضة الى دور الحشرة الكاملة وحساب معدلات الهلاك التراكمية للادوار غير البالغة وحساب معدلات الهلاك باستخدام مبيد بولو 500 واوضحت النتائج ان النبات والمبيد المستخدمين في هذه الدراسة لهما تاثير كبير في حياتية ذبابة التبغ البيضاء وكما يلي : A - المستخلصات النباتبة : تمثل نسب هلاك البيوض اقل المستويات مقارنة مع الاطوار الاخرى اذ كان مستخلص خلات الاثيل الاكثر تاثيرا ، اذ بلغ معدل الهلاك %83.67 يليه المستخلص الكحولي 70.60% واقل المستخلصات تاثيرا المركبات الفينولية بلغ معدل هلاكها 48.5% .كانت اغلب المستخلصات المستخدمة ذات تاثير كبيرفي هلاك الطور الحوري الاول ، اذ بلغ اعلى معدل للهلاك باستخدام المستخلص الكحولي وبلغ 95.87% يليه خلات الاثيل بمعدل 78.52% واقل معدل للهلاك كان عند استخدام المستخلص المائي بمعدل 49.57 %.تفوقت المستخلصات النباتية الاتية في الطور الحوري الثاني وهي : الكحولي والهكسان وخلات الاثيل والقلويدات وبمعدلات هلاك 96.33 و89.74% و76.93 و72.27 %على التوالي،اما المستخلص المائي فكان الاقل تاثيرا اذ بلغ معدل الهلاك 55.9%.اما الطور الحوري الثالث كانت اغلب المستخلصات النباتية والمركبات الفعالة ذات تاثير كبيرفي معدل هلاك هذا الطوراذ بلغت معدلات الهلاك93.29% للمستخلص الكحولي و82.83% لمستخلص خلات الاثيل و75.55% للقلويدات واقل معدل للهلاك سجل عند استخدام المستخلص المائي وبلغ %59.54 .سجل الطور الحوري الرابع (العذراء) اعلى معدل للهلاك عند استخدام المستخلص الكحولي وكانت النتيجة 91.41% تليه المركبات الفينولية 89.28% واقل معدل للهلاك سجل عند استخدام المستخلص المائي بلغ %59.12 .وكان اعلى معدل لهلاك الحشرة الكاملة عند استخدام المركبات القلوانية بمعدل90.94% تليها المركبات الفينولية بمعدل 89.93% وخلات الاثيل بمعدل89.85 % والكحولي 87.69% والهكسان 80.17% واقل معدل للهلاك عند استخدام المستخلص المائي وبلغ معدل الهلاك %69.03. - B معدل الهلاك التراكمي : اوضحت النتائج تفوقا واضحا للمركبات القلوانية في معدل الهلاك التراكمي للادوار غير البالغة لذبابة التبغ البيضاءB. tabaci يليها مستخلص الهكسان ومستخلص خلات الاثيل والمستخلص الكحولي واقلها كان المستخلص المائي . - C مبيد بولو 500 : تبين من خلال النتائج ان استخدام مبيد بولو500 له تاثير كبير في معدل هلاك ذبابة التبغ البيضاء من دور البيضة لحين الوصول الى الحشرة الكاملة ،اذ بلغت معدلات الهلاك كما يلي : البيوض 58.51% والطور الحوري الاول 44.70% والطور الحوري الثاني 64.41% والطور الحوري الثالث 70.49% والطور الحوري الرابع (العذراء) 73.48% واخيرا الحشرة الكاملة 72.30 %. | The current study was conducted in order to evaluation efficiency of plant extract and effective compounds to plant Vitex asgnus - castus L. on the biological performance of Bemisia tabaci(Genn.)whitefly through calculating mortality rate for each treatment with different concentration and period from egg stage until adult stage and calculate the cumulative mortality rate for immature stages and calculate mortality rate by use Polo500 insecticide Ressults demonstrated that the plant and insecticide used in current study had more effective important role in life of the B. tabaci as follow A - Plant Extract Mortality rate of egg : represent ratios of egg mortality in the lower levels compare with other stages , mortaiity rate of ethyle acetate extract has more effective (83.67%) than alcohol extract mortality rate (70.60%) and lower extract effective was phenol compounds mortality (48.50%) First Nymphal instar : in this instar most of used extracts had more effective in mortality of this instar, more mortality rate were by alcohole extract (95.87%) the ethyl acetate (78.52%) and lower mortality rate was by aqueous extract (49.57%) Second Nymphal insrar : most effective was to following plant extracts; Alcohol, Hexan , Ethyl acetat , Alkaloid with mortality rate (96.33%) , 89.74% , 76.93% , 72.27% Aqueous extract has the lower effective mortality rate 55.90% Third Nymphal instar : most plante extracts and effective compounds had large effect in mortality rate in this stage (93.29%) for alcohol extract , 82.83% for ethyl acetat ,75.55% for alkaloid and lower effective was to aqueous extract 59.54% Fourth Nymphal instar ( pupa) : in this stage alcohol extract was more effective in mortality rate 91.41% , then phenol compound 89.825 and lower perdition average was to aqueous extract 59. 12% .Adult : the highest mortality rate was when used alkaloid compounds 89.93% , then ethyl acetat 89.85% , alcohol 87. 69% , Hexan 80.17% and lower average to aqueous extract 69.03% .B - The accumalative perdition : Results demonstrated more effective to alkaloid compounds in inmature stage of B.tabaci , then to hexan extract , ethyl acetat , alcohol extract and lower average was to aqueous extract .C - POLO500 Insecticide : Results show this insecticide has significant effect in motality rate of B. tabaci starting from egg to adult . egg 58.51% , first instar 44.70% ,second instar 64.41% , third instar 70.49% , pupa 73.48% and adult 72.30%

دراسة التغيرات النسجية المرضية في اعضاء سمكتي البلطي الزيلي Coptodon zillii والكارب البروسي Carassius auratus في بعض انهار ابي الخصيب وعلاقتها ببعض العناصر الثقيلة == Study of Histopathological changes in organs of fish, Coptodon zillii and Carassius auratus in Abo Alkhaseeb Rivers and relationship with some type of heavy metals

Author name: خالد عبد الصمد عبد الرضا السلمي
Supervisor name: علي عبد اللطيف عبد الحسن العلي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة الحالية جمع نوعين من الاسماك النهرية هي سمكة البلطي الزيليCoptodon zillii والكارب البروسي Carassius auratus من اربعة انهار في قضاء ابي الخصيب وهي نهر( السراجي وحمدان ومحيلة وابو مغيرة )وعلى فترتين ,امتدت الاولى من شهر حزيران 2013 ولغاية شهر تشرين الثاني 2013 وامتدت الثانية من شهر كانون الاول 2013 ولغاية شهر مايس 2014 . اجريت الدراسة الحالية بمحورين شمل المحور الاول دراسة التغيرات النسجية المرضية في اعضاء الغلاصم والكبد والكلية والعضلات وللسمكتين اما المحور الثاني فتضمن دراسة تراكم اربعة من العناصر الثقيلة(الرصاص والنحاس والنيكل والكوبلت) في انسجة الكبد والعضلات وذلك لغرض ايجاد علاقة بين المحورين . قد بينت الدراسة النسجية للاعضاء المدروسة ظهور تغيرات نسجية مرضية اختلفت في شدتها بين نوعي الاسماك اذ تبدو انسجة اسماك الكارب اكثر تضررا من انسجة اسماك البلطي فضلا عن اختلافها بين افراد النوع الواحد , ففي الغلاصم تمثلت التغيرات المرضية بتغير شكل الصفائح الغلصمية الثانوية وفرط تنسج hyperplasia النسيج االظهاري وانفصاله detachment ولوحظ تضخم hypertrophy الخلايا الظهارية وانتفاخها swelling وتضخم وزيادة اعداد خلايا الكلورايد فيها فضلا عن ظهور فرط تنسج الغضروف في الهيكل الساند للخيط الغلصمي الذي رافقه في بعض الاحيان تجمع خلايا بيضوية طرفية النواة في غلاصم اسماك الكارب . في الكبد تمثلت التغيرات المرضية باحتقان congestion اشباه االجيوب الكبدية وتليفات fibrosis في نسيج الكبد وتنكس degeneration وتنخرnecrosis الخلايا الكبدية وتغيرات نووية تمثلت بانحلال النواة وتكتل مادتها الكروماتينية karyopiknosis ولوحظ وجود ودمات oedema وتراكم مواد غير حية بنية ووردية اللون داخل وخارج الخلايا الكبدية . اما في الكلية فقد شملت التغيرات المرضية النسجية جميع مكوناتها اذ تمثلت بتنكس الخلايا الظهارية للنبيبات الكلوية وانتفاخ بعض منها مما يؤدي الى ضيق تجويف النبيب ولوحظ حدوث انسلاخ بطانة بعض النبيبات الكلوية , وظهر فرط تنسج النبيبات الكلوية في كلية اسماك البلطي فقط بشكل كتل كبيرة بيضوية الشكل , ولوحظ وجود مواد غير حية في النسيج المكون للدم يحاط البعض منها بمحفظة ليفية من عدة طبقات في سمكة الكارب ,اما في الكبيبات فقد لوحظ احتقان وتوسع aneurism اوعيتها الدموية, وشوهد ضمورatrophy بعض الكبيبات وتضخم بعضها الاخر مما يؤدي الى توسع او تضيق حيز محفظة بومان. تمثلت التغيرات المرضية النسجية في الامعاء بتنكس خلايا النسيج الظهاري وتنخر قمم الزغابات ولوحظ ان التنكس والتنخر يصل في بعض العينات الى النسيج الضام للصفيحة الاصيلة وشوهد ارتشاح infiltration الخلايا البدينة mast cells باعداد كبيرة في الطبقة تحت المخاطية لامعاء اسماك البلطي وارتشاح انواع اخرى من الخلايا الالتهابية في اسماك كلا النوعين , وكثيرا ما شوهد تاثر الخلايا العضلية في طبقة العضلات لجدار الامعاء. اما انسجة العضلات فقد شهدت ظهور العديد من التغيرات تمثلت بتنكس اسفنجي spongy degeneration يتبعه تنخر الخلايا العضلية وكثيرا ما شوهد تعرج الليفات العضلية وانعدام التخطيط العرضي . قد تعزى هذه التغيرات الى تلوث مياه الانهار المدروسة بمختلف الملوثات ومنها المعادن الثقيلة وذلك من خلال قياس التراكم الحيوي لهذه العناصر في عضلات واكباد هذه الاسماك واكد وجود هذه العناصر تقنية الكيمياء النسجية اذ لوحظ ان المعادن المتراكمة في النسيج تعطي انعكاسات لونية مختلفة بحسب نوع العنصر الثقيل ,و لوحظ عند قياس التراكم الحيوي ان معدلات تراكم بعض هذه العناصر(الرصاص والكوبلت) في انسجة العضلات يفوق الحدود المسموح بها عالميا . اختلف تراكم هذه العناصر من عضو الى اخر ومن نوع الى اخر ومن فترة الى اخرى ,فقد وجد ان معدلات تراكم عناصر الرصاص والنيكل والكوبلت في العضلات كان اعلى من معدله في الكبد وبفرق معنوي عند مستوى احتمالية p<0.05 في حين اظهر النحاس نتيجة معاكسة اذ كان معدل تراكمه في الكبد اعلى من العضلات وبفرق معنوي عند مستوى احتمالية p<0.05 , اما بالنسبة لفترتي الدراسة فقد سجلت الفترة الاولى في كبد سمكة البلطي معدلات تركيز اعلى من الفترة الثانية لكل من عناصر النحاس والنيكل والكوبلت وبفرق معنوي عند مستوى احتمالية p<0.05 . اما في مياه الانهار المدروسة فقد كان اعلى تركيز بلغه عنصر الرصاص 602 مايكروغرام\لتر واقل تركيز له 141 مايكروغرام\لتر,واعلى تركيزلعنصر النحاس كان 77 مايكروغرام\لتر واقل تركيز 25 مايكروغرام\لتر, واعلى تركيز لعنصر النيكل كان 392مايكروغرام\لتر واقل تركيز له214 مايكروغرام\لتر,واعلى تركيز لعنصر الكوبلت كان 173مايكروغرام\لتر واقل تركيز72مايكروغرام\لتر | The current study includes collection of two species of fish. a tilapia fish (Coptodon zillii) and Prussian carp ( Carassius auratus) from four rivers in Abu Alkhaseeb region which are Sarraji, Hamdan, Mhella and Abu Mugherra River at two periods, the first period starts from June 2013 till November 2013 and the second period from December 2013 to the end of May 2014. After bringing the fish to the lab dissecting to rescet excised organs of the gills, livers, kidneys , intestines and muscles. the current study have been performed on two axis .The first axis includes the study of histopathological changes in the organs above for two species The second axis guarantees the study of the bioaccumulation of four heavy metals (lead ,copper, nickel ,cobalt) in the tissues of the liver and muscles for the purpose of finding a relationship between the two axes. The histological observations showed histopathological changes different in severity between the two specis of fish , the tissue of C.auratus seems to more be affected than the tissue of C.zillii , In the gills, the pathological changes are represented in the shape changes of secondary lamella of gills , hyperplasia and detachment of epithelial tissue . Hypertrophy and swelling in epithelial cells , hypertrophy and increase the number of chloride cells where noted . Hyperplasia cartilage in Bracket structure of gills thread which coincide with some gathering terminal oval cell nucleus often in gill, carp was seen. In the liver has represented pathological changes like congestion of liver sinusoid , fibrosis in liver tissue , degeneration and necrosis of hepatocytes and the nuclear changes represented in the dissolution of the nucleus and conglomerate chromatin material ( karyopiknosis), the appearance of some oedema and accumulation of non - living material inside and outside the hypatocytes . In kidney ,the histopathological changes included represente by epithelial cells degeneration of the renal tubules and swelling some of them leading to a narrow tubules cavity, also observed shedding the epithelial layer lining of some renal tubule, while in the glomeruli, congestion and aneurism its veins atrophy of some glomeruli was obsererved and that leads to expansion or narrowing Bomans space . In addition non - living materials in the component tissue of blood were observed, some of which are surrounded by a fibrous capsule of several layers in C.auratus kidney only. Some of non - liver materials in the cytoplasm of some of renal tubule cells were seen, while, intestinal histopathological changes were represented by degeneration of epithelial cells and necrosis of villi peaks and it was observed that the degeneration and necrosis reach in some samples to the connective tissue and often spotted muscle cells affected in the muscle layer of the intestinal wall. Also it is observedin a large numbers of mast cells infiltration in the submucosa layer of the gut C.zillii and infiltration other types of inflammatory cells in both fish species, while muscle tissue were less affected than the other organs. There were several changes represented by spongy degeneration followed by necrosis of muscle cells , and often were seen in the limping of muscle fibers and the lack of cross - planning in this muscle. These histological changes were due to water pollution of rivers under studing by various pollutants such as heavy metals that were included in thestudy and that by measuring the bioaccumulation of heavy metals in the tissues of the muscles and liver of these fish and was assured that by Histochemistry technique as it was observed that the accumulated metals in the tissues give different reflections of color by heavy element type. It was observed that when measuring bioaccumulation , the accumulation rates of some of these elements (lead and cobalt) exceeds the permitted globally border. It was observed that the accumulation of these elements vary from one organ to another and from one specis to another and from one period to another, it was found that the rates of accumulation of elements ;lead , nickel and cobalt in the muscle were higher than that in the liver and at significant difference of 0.05 level of probability, while copper showed the opposite conclusion, as its rate of accumulation in the liver was higher thane at significant differenc of 0.05 level of probability. Regarding the two periods of the study, the first period recorded higher concentration in the liver of C.zillii than the second period for copper ,Nickel and cobalt elements at significant 0.05 difference are level of probability. . The rates of concentration of heavy elements that included in the study of the water of the rivers were nearly the same to former studies, as the highest concentration recorded for lead was 602 micrograms \ liter and the less concentration was 141 micrograms \ L, while the highest concentration of copper was 77 micrograms \ liter and less concentration was 25 micrograms \ L. The highest concentration of the nickel was 392 micrograms \ liter and its less concentration was 214 micrograms him \ l. The finally highest concentration of cobalt was 173 micrograms \ liter and its less concentration was 72 micrograms \ L

الكساء الخضري والتنوع الاحيائي النباتي في منطقة الصحراء الجنوبية في محافظة البصرة / جنوب العراق == Vegetation and Plant Biodiversity in the Southern Desert in Basra Governorate, Southern Iraq

Author name: حيدر راضي مالح حسين
Supervisor name: عبد الرضا اكبر علوان المياح
General topic: Biology
Specific topic: Plant - Environment
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تناولت الدراسة الحالية الغطاء النباتي والتنوع الاحيائي والمجتمعات النباتية في منطقة الصحراء الجنوبية ضمن محافظة البصرة, وتم دراسة الخصائص الكمية والنوعية للغطاء النباتي , فضلا عن خصائص الترب والهواء في 12 محطة, 6 منها خصصت للمسح الشهري للانواع للفترة بين كانون الاول 2012 وكانون الاول 2014.تم دراسة الخصائص الفيزيائية والكيميائية للعوامل البيئية في منطقة الدراسة وشملت حساب قيم درجة حرارة الهواء والرطوبة النسبية, وكذلك دراسة خصائص الترب ومنها قوام التربة ودرجة حرارة التربة ورطوبة الترب والاس الهيدروجيني والمادة العضوية والتوصيلية الكهربائية وقيم الملوحة, وتبين انها اظهرت تباينا موسميا في المحطات الستة. اوضحت الدراسة الحالية وجود 185 نوع و136 جنس تعود الى 41 عائلة, منها 159 نوع و115 جنس و36 عائلة تعود الى مجموعة نباتات ذوات الفلقتين, في حين ضمت مجموعة نباتات ذوات الفلقة الواحدة 26 نوع تقع في 21 جنس و5 عائلات, وكانت اغلبها نباتات حولية حوالي 66% , بينما كانت نسبة النباتات المعمرة 44 %, كما تم تسجيل حوالي 20 نوع لاول مره في مناطق الدراسة, كما اكدت التوزيع الجغرافي وسجلت انتشار العديد من الانواع في مناطق لم تسجل فيها سابقا.وبينت دراسة اشكال الحياة في منطقة الدراسة ان مجموعة Therophytes قد امتلكت اعلى نسبة بلغت 71.19%, بينما ضمت مجموعة ال Geophytes اقل نسبة بلغت 4.3 %. كما تغايرت الاصول الجغرافية النباتية Chorotype للانواع,عكست اهمية الموقع الجغرافي لمنطقة الدراسة, وسجلت العناصر ثنائية وعديدة المناطقBi & Pluri regional 70.7 elements % منها, وشكلت العناصر الصحراوية - العربية Sahara - Arabia elements نسبة 20.54%.وبينت النتائج ان اكبر العائلات انتشارا وتوزيعا وبعدد الانواع في منطقة الدراسة هي العائلة المركبة Compositae التي تعد اكبر العائلات في المنطقة, ثم العائلة الصليبية Cruciferae , والعائلة النجيلية Gramineae , والعائلة البقوليةFabaaceae , والعائلة الرمرامية Chenopodiaceae , كما تبين ان الاجناس Astragalus وPlantago وErodium كانت اكبر الاجناس بعدد الانواع في منطقة الدراسة, واظهر ت دراسة التوزيع الجغرافي ان بعض الانواع سجلت انتشارا واسعا في مناطق الدراسة ومنها Hammada salicornica Moq. وRhanterium epapposum Oliv.و Stipa capensis Thunb. وIflago spicata (Forssk) Sch. Bip. وFilago pyramidata L. وSpegularia diandra (Guss.) Heldr & Sart. وStrigosella grandiflora ( Bunge.) Boch. .كما تضمنت الدراسة وصف وتمييز المجتمعات الرئيسة وتحديد مناطق انتشارها في مناطق الدراسة وضمت Hammadetum salicornici وCornulacetum auchraeو Rhanterietum epapposi وZygophyllietum propinqun وAstragalietum spinosi وSalsoletum setiferaeو Convolvulietum oxyphylliو Salsoletum rosmarini وStipetum capensiو Ziziphetum nummulariaeو Teucriutum oliveriو Stipagrosis plumosi وLycetum barburae وSuaedetum aegyptiaci . واظهرت الدراسة التغيرات الموسمية في طبيعة الغطاء النباتي وتاثر السيادة بالتغيرات الموسمية في الظروف المناخية والتي ساهمت في ظهور المنطقة بعدد من المظاهر الموسمية للغطاء الخضري ( Seasonal Aspect) . وبينت نتائج الدراسة الحالية وجود تباين موسمي ومكاني بين غالبية محطات الدراسة في قيم ادلة الكثافة والوفرة والتغطية والتنوع والسيادة والتجانس والغنى النوعي والتشابه والكتلة الحية والتصاحب والاهمية.

دراسة تعدد الاشكال الوراثية لجين GSTPI في مرض السكري من النوع الثاني لعينة مرضى في محافظة البصرة وعلاقتها بدهون مصل الدم == Genetic Polymorphism of Gultathion - S - Transferase GSTPI in Type2 Diabetes Mellitus and it's Correlation with Blood Serum lipid Profile in Basrah Province

Author name: حنين صباح عبد الصمد
Supervisor name: فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was carried out to investigate the relationships between the T2DM patients and the genotypes of glutathione S - transferase P1, and the measurements of blood serum lipid profile of patients and control. The study included 60 blood samples from patients and 40 blood sample from controls of Iraqi individuals. The sixty individual with T2DM were diagnosed according to the American diabetes Association criteria (American diabetes Association, 2007), their age ranged were between 35 - 75 years randomly selected from those attending the Diabetes Center /Al - Moanaa Hospital for treatment with history of hyperlipidemia and hypertension. Sera and blood were used as sample, The measured parameters in serum included (Biochemical testes) : Fasting Blood Glucose (FBG), Total Cholesrtol (TC), Triglyceride (TG), low density lipoprotein (LDL), High density lipoprotein (HDL), Very high density lipoprotein (VLDL). In Addition to Body Mass Index (BMI) .Moreover, the correlation between genotyping of glutathione S - transferase P1 and all studied parameters was carried out. Molecular studies involved DNA extraction and Polymerase chain reaction (PCR) - Retraction fragment length polymorphism (RFLP). to investigate the genetic polymorphism of glutathione S - transferase pi gene class for individuals of this study .Molecular study was conducted in the laboratory of molecular Biology, Department of Biology for pure Sciences college at Basra University .The result showed that Sex, BMI, HDL, LDL and VLDL values for patients and control were not significant while for the level of FBG in patients and control it showed significantly difference (P=0.000). This study revealed significant Positive Correlation between cholesterol and triglyceride (P=0.01). while the Correlation coefficient between Triglyceride & HDL,VLDL individually also between HDL and LDL ,VLDL and LDL with VLDL individually was not significant furthermore FBG and Cholesterol showed positive significant Correlation .The result of genetic polymorphism of GSTPI distribution among studied groups showed rise developing risk of Type 2 Diabetes in patients about 2 - fold in carries heterozygous Ile/Val genotype with an ( OR=2.90; 95% Cl= 1.077 - 7.827, p=0.31) and allele frequency 0.69.,and there is an increased risk of Type 2 Diabetes in patients about 2and half - fold in carries of The homozygous Val/Val genotype in patients with an (OR=2.58, 95%CI=0.625 - 10.662, p=0.171). the heterozygote distribution (2Pq ) of Patients was 0.427 while in control 0.268 and it was not stable according to Hardy - Winderg question.According to data mentioned above the GSTPI genotype polymorphism may play an important role in the pathogenesis of T2DM while its effect on all lipid profile indicator did not show any significant difference.

دراسة جزيئية لتعدد الاشكال الوراثية لجين TAS2R38 (جين تذوق مادة PTC) بين سكان محافظة البصرة وعلاقتها بمرض السكري == A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes Mellitus

Author name: حسنة عامر مهوس
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study dealt with the genetic polymorphism of Phenylthiocarbamide (PTC) tasting from phenotypic and genotypic (Molecular of TAS2R38(P49A) gene ) prospective,among sample of Basrah Population from 2014 - 2012. Also, a comparative Molecular study for the gene TAS2R38(P49A) and some biochemical parameters was made in a group of Diabetic patients for the first time in Iraq /Basrah and the world .Phenotypic study using the gradient dilutions of PTC substance method, was established among 317 individual : 96Males and 221 Females from different regions of Basrah. Increased sensitivity with low threshold 6 of Males than Females 5 was observed which indicate that the Males were more sensitive than Females in this region from Iraq .However, the tasters percent among Females still higher73.3% than Males72.9% ,and the whole population 73.2% as well .This study recorded different tasters percent in different regions : 62.1%,71.8% ,60.6%, 82.3% for the North ,AL - Zubair and Safwan, Abu - ALChaseab and AL - Fao and the center of the city respectively, although ,the whole population of Basrah found to be under Hardy - Weinberg Equilibrium . Allelic frequencies according to phenotypic analysis showed : recessive allele t had higher frequency (0.53) than dominant allele T (0.47), with elevated Heterozygous frequency Tt among all studied regions and the whole population(0.53) .The results recorded also , high BMI for non tasters tt as a whole and for both sexes in this phenotype : 28.32 ,28.26 for males and females respectively. While it had the lowest value among heterozygous 23.40 ,Statistical analysis for phenotypic study of this locus reveald an additive effect of the two alleles T,t by 81.33% with responsibility of 83.10% for the phenotypic variation found in this trait . Genetic (Molecular) analysis was done for comparative individuals and diabetic patients by sequencing the hTAS2R38(P49A) the PTC gene, among 127 individual : 59 healthy individual as a comparative group and 68 diabetic patients from both sexes and same age ranges. The results recorded a C/G allele in the position of the first SNP (145 C/G) which encode for Proline amino acid (C) or Alanine amino acid (G) : CC , CG refer to Tasters genotypes while GG refer to Non - tasters genotype. High frequency of C allele as compare to G among both healthy people and diabetic patients : 0.55, 0.53 for both respectively . Frequency of heterozygous genotype CG recorded a significant access as 0.76 among the comparative group which may implies a selective heterozygosity advantage among Iraqi population at this region. A significant differences in genotypic distributions was recorded between comparative and diabetic groups with high incidence of non tasters phenotypically tt 41% and genotypically GG 20.5 % among diabetic patients compare to healthy people : 25 % , 7% respectively . Interestingly non taster GG genotype had the lowest BMI among diabetic patients compare to othertwo taster genotypes CC, CG respectively. . Study of Genotype - Phenotype Association revealed significant correlation (p<0.0001) between them among comparative group as 86.9% and diabetic group as 48.8%. A comparative study of Biochemical analysis betweendiabetic and non diabetic individuals from different genotypes for TAS2R38 (P49A) gene showed significant elevated value of cholesterol among diabetic from the GG genotype 191.85 ± 6.57 mg/dl with non significant elevated values of Hb1c 8.93 ±1.77 and triglyceride 149.57±6.53 mg/dl. However, all these parameters were significantly more higher among diabetic than non diabetic . Significant differences(P<0.05) were recorded between diabetic and non diabetic according to their thyroid hormones levels with low values of T3 , T4 and high values of TSH among diabetic than non - diabetic. TSH hormone recorded its highest significant values among diabetic of CC genotype with 4.39 ± 0.16 μUI/ml as compare to the other two genotypes heterozygous CG and recessive homozygous GG . Although non significant , T4 recorded its lowest values among diabetic of the CC genotype .However, significantly (P <0.05 ),T3 hormone found to have lower values in the two homozygous genotypes; CC genotype for diabetic 0.54± 0.03 ng/ml and non diabetic 0.53±1.64 ng/ml ; GG genotype : 0.72± 0.04 ng/ml ; 1.82± 0.59 ng/ml for diabetic and non diabetic respectively . while individuals with CG genotype found to be non significantly different .Lastly it could be concluded that this trait may have some effect on BMI and may play a role the variation found among peoples in biochemical parameters (cholesterol, thyroid hormones ) changing's especially among diabetic patients. Ministry of High Education and Scientific Research Basrah University/ College of Education for Pure Science Department of Biology A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes MellitusA Thesis submitted to the Council of the College of Education for Pure Science/ University of Basrah as a partial fulfillment of the requirements for the degree of Philosophy of Doctor of Science in Biology / Genetics By Hasna Amir Mohaus BSc. Biology / 1994MSc. Biology / 1998 Supervision by Assist.Prof.Dr. Prof.Dr.Faizah A.

دراسة بعض التغيرات الفسيولوجية والنسجية لسم الافعى ذات الحراشف المنشارية (افعى سيد دخيل Echis carinatus) في الجرذان المختبرية == A study of some physiological and histological effects of venom Saw Scaled Viper (Said Dakhil) Echis carinatus in laboratory rats

Author name: بيداء ريحان علي الموسوي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was designed search of some physiological and histological effects of the venom of Echis carinatus sochureki (Said Dakhil snake) in male and female rats, and the role of antivenom (anti - venom) in the treatment of these effects. The while study was divided into two parts, the first part included the effect of [0.04 mg/kg (the second group) and 0.08 mg/kg (the third group)] of venom in rats. While, the second part related with the effect of [0.25ml/animal (the second group) and 0.5ml/animal (the third group)] antivenom in male and female rats treated with 0.04mg/kg and 0.08mg/kg of venom. After 24 hours of injection, blood samples collected for the hematological and biochemical parameters, and taking the right epididymis and epididymis left removed to examine the count and maliformations of sperm. Also, liver, kidney, testes and ovary were collected for sections. The results of the present study showed a significant decrease (P≤0.05) in RBC in the third group (0.08) mg / kg when compared with the first group (control) and second group (0.04) mg / kg. Also was there a significant decrease in HCT and MCV in the second and third groups compared with the first group, while there was a significant increase in the platelets of the third group compared with the first and second groups in in both sexes. The results indicated a significant decrease in RBC in the second and third groups compared with the first group, also a significant decrease in HCT in the second and third groups compared with the first group. The results showed a significant increase in the HCT in the second group compared with the third group. The results showed a significant decrease in the MCV in the third gr oup compared with first and second groups in female rats. The present study (P≤0.05) showed a significantdecrease in total WBC, lymphocytes and neutrophils in the second groups (0.04) mg / kg and the third (0.08) mg / kg, and a significant decrease in monocytes, acidiophils and basophils of the second and third groups compared with the first in the male rats. The results indicated a significant decrease in neutrophil in the second and third groups compared with the first group, and a significant decrease in lymphocytes, monocytes and acidiophils of the third group compared with the first and second groups in the female rats. The present study showed a significant increase (P≤0.05) in the level of glucose and urea in the third group (0.08) mg / kg compared with the first (control) and second (0.04) mg / kg groups. There was a significant increase in creatinine and albumin in the second and third groups compared with the first group, also a significant decrease in the total protein, cholesterol and HDL in the second and third groups compared with the first group.There was a significant decrease in the level of TG, LDL and VLDL in the third group compared with the first and second groups, The results showed a significant decrease in ALT and AST in the third group compared with the first and second groups, and a significant increase in the ALP in the third group compared with the first and second groups in the male. The hormonal study showed a significant decrease (P≤0.05) in the level of FSH, LH, testosterone and estrogen in the second and third groups compared with the first group in male and female rats. The present study a significant decrease (P≤0.05) in the count of sperms in the second and third groups compared with the first group, while, there was a significant increase in the maliformations of sperms in the third group compared with the first and second groups. The present study showed a significant decrease (P≤0.05) in the level of immune globulins (IgA - IgG - IgM) in the second and third groups compared with the first group in both sexes. The results of the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated with (0.04) of snake venom showed a significant decrease in RBC and HCT in the second and third groups compared with the first groups,and there was a significant increase in the MCV in the second and third groups compared with the first group in male. The study results showed a significant increase in RBC, HCT and MCV in the second and third groups compared with the first group in the female rats.The current study showed a significant decrease (P≤0.05) in total WBC of the second group compared with the first and third groups, and a significant decrease in the lymphocytes and monocytes in the second and third groups compared with the first group, also the results showed a significant increase in total WBC in the second and third groups compared with the first in the male. Also, found a significant decrease in lymphocytes and monocytes in the secon d and third groups compared with the first, it was observed a significant rise in monocyte in the first group compared with the second and third groups. The results showed a significant increase in the percentage of granular white blood cells in the second and third groups compared with the first group in the female rats.Results of the present study indicated a significant decrease (P≤0.05) in the level of glucose, urea, albumin and total protein in the third group compared with the first and second groups, and there was a significant increase in the level of cholesterol HDL - VLDL in the third group compared with the first and second groups, also a significant decrease in TG in the second group compared with the first and third groups, The results showed a significant decrease in the level of HDL in the second and third groups compared and second groups with the firstgroup. the results showed a significant increase in the level of albumin and total protein in the third group compared with the first and second groups, and the results indicated a significant in increase cholesterol level, LDL and VLDL in the third group compare with the first and second groups, There was a significant decrease in the level of TG in second group compared with the first and third groups, and a significant decrease in the level of HDL in the second and the third groups compared with the first group in the female rats.The results indicated the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated (0.08) mg / kg of snake venom, showed a significant decreased (P≤0.05) in RBC, HCT , MCV, lymphocyte and monocyte in the second and third groups compared with the first group, a significant decrease MCH in the second group compared with the first and third groups in male rats, showed a significant decrease in RBC HCT and MCV in the second and thirdgroups compared with the first group.The present study showed a significant decrease (P≤0.05) in the level ofglucose and urea, ALT and ALP in the third group compared with the first and second groups, also a significant decrease in the level of creatinine, LDL, VLDLand AST in the second and third groups compared with the first group, and asignificant increase in the level of albumin, total protein and HDL in the thirdgroup compared with the first and second groups. a significant increase in levelof cholesterol and TG in the second and third groups compared with the firstgroup in the male rats, While showed a significant decrease in level of glucose,urea and ALT in the third group compared with the first and second groups,there was a significant decrease in the level of creatinine, LDL, VLDL, AST and ALP in the second and third groups compared with the first group, a significant increase in albumin and total protein in the group third compared with the first and second groups, while a significant increase in level of cholesterol, TG and HDL in the second and third groups compared with the first group in female.The histological results showed changes in the liver of male and female rats treatment I n venom snake and constrictions consisted obtaining inflammation and blood congestion and enlarged nuclei of hepatic cells and degeneration in the cytoplasm and necrosis of liver cells, as well as, changes in the kidney tissue such as having a bleeding and hyperplasia and congestion blood and inflammation and analyze the entire glomerulus and necrosis of the cortical cells.The current study showed the incidence and clear changes in testicular tissue treatment venom snake of obtaining vascular congestion and analyze the interconnection fabric between the tubule and the decay of some nuclei and abnormalities tubule and testicular and shrinking and analyzes and the presence of multiple nuclei giant cells in the wall of tubule.The current study for histological changes in the ovary as sections showed the presence of large numbers of corpus luteum and the decrease numbers of ovarian follicles and the disappearance of the evolutionary stages of ovarian cysts.

تاثير PTU على بعض الجوانب النسيجية والفسلجية في الاجنة والاناث الحوامل Rattus norvegicus == Effect of PTU on some aspects of histological and physiological in fetus and pregnant rats(rattus norvegicus

Author name: انوار ناذر صيوان
Supervisor name: مها خليل الملاك | علاء عبد الخالق حسين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: Aim of study was to evaluated the effects of propylthiouracil (Ptu) drug on both maternal and fetus rats, used experimental animals (Rattus norvegicus) and after housing, breeding and adapting, (50) of virgin females choosen, isolated and then divided into two groups each group contain (25)rats, The mean weight of the animals was(200 ±50) gm and (8 - 10) week of age. Females on first group regarded as control and treated with distilled water while animals in second group were treated with (Ptu)dose as (0.05 %) and left for(14.5, 16.5, 18.5, 20.5, 21.5)for each group. Macroscopic observations on each group were recorded including changes in thyroid gland morphology, hypertrophy and enlargement at each period (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation for both pregnant rats and embryos,the shape of uterine hornes,and uterus with changes of fetus number, resorption, atrophy, malformation of body parts, indifferentiation of fore and hind limbs, skin abnormalities were noticed. The changes in placenta which appeared irregular, pale colour, congested with white fibrous regions were noticed in hypothyroid rat comparing with placenta of control group animal, which looked red, discoid shape and normal surface without any abnormal signs, in addition fatty ovaries with large amount of adipose tissue was accumulated in abdomen cavity, the ovaries irregular and showed structures in most pregnant rats of hypothyroid group comparing with control pregnant females.Present showed significant decreased at (P≤0.05) in mean length, weight and number of fetus at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation in all embryos related to hypothyroid rats compared to control. Histological study with light microscopy on sections from thyroid gland related to rats with induced hypothyroid showed reduced, small follicles with empty or little colloid substance, each follicle lining simple cuboidal or flat epithelium, an increased with stroma, variable size of follicles most of them smaller compared to control thyroid gland from control rats, which showed normal lobules, large follicles filled with colloid, and poorly stroma.Sections from uterus, ovaries, oviduct related to hypothyroid rats showed variable histological changes ranged from mild to severe like abnormal reduced layers of uterus, irregular uteri folded, degenerated with simple epithelial tissue that lining the uterus villi, haemorrhage in uterine gland, deposition of collagenous fibers, infiltration of lymphocytes and accumulation of adipose tissue. Ovaries from hypothyroid rats showed variable changes with ovaries follicles maturation, accumulated of adipose tissue, decreased graffian follicles, infiltration of lymphocytes and deposition of collagenous fibres with degenerated of lining epithelial layer compared with control ovaries.Microscopic observations of placenta sections from hypothyroid rats at different period of gestation revealed loss tissue, reduced placental layers thickness, necrosis in the peripheral regions, degenerated cytotrophoblasts that surrounding capillaries, the trophoblast giant cells appeared with degenerative changes in labyrinth and spongiotrophoblasts regions. undifferentiated fetal blood capillaries compared to control which the sections have normal structure differentiated to decidual basalis and labyrinth zones, the junctional zone composed of outer giant cells that separated the decidua basalis and trophospongium.In this study the ultrastructural changes in thyroid gland from maternal and fetus related to hypothyroid rat at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation were identified by electron microscopy and included changes in apical cytoplasm of follicular cells to microvilli, blebs and protrusions also dilated rough endoplasmic reticulum, increased lysosomes, the empty vacuoles more than the dense vesicles, increased mitochondria and Golgi apparatus cisternae, changes chromatin and more cellular debris. Biochemical results in thyroid hormones revealed to significant increase in mean concentration of (TSH) in pregnant females with hypothyroidism during gestation periods in compared with control group, also there was an decrease in both (T3, T4) non significant, significant increased concentration in serum of pregnant rats on treated group with (Ptu) compared with their concentration of control pregnant rats.Levels of some oxidative enzymes (GSH & MDA) was estimated in this study and there was significant increased,non significant in their concentrations in serums of rats with induced hypothyroidism comparid to control group during each period of gestation (14.5, 16.5, 18.5, 20.5, 21.5) day. The study determined some of haematological parameters such as (R.B.Cs) count, (H.b) level and (P.C.V) ratio which showed non significant at (P≤0.05) hypothyroidism rats compared with control rats at the same period of gestation. Also the results revealed non significant with (T.L.C), (D.L.C) in all pregnant rats with hypothyroidism compared to control rats, the data showed an increased with lymphocytes and Neutrophils and Esoinphils cells in smears prepared from hypothyroidism rats compared to control group

بعض الجوانب الحياتية للروبيان الدخيل والروبيان Macrobrachium nipponense (De Haan,1849) من Metapenaeus affinis (H. Milne Edwards, 1837) بعض المواقع المائية في محافظة البصرة == Some Biological aspects of the Invasive Shrimp Macrobrachium nipponense (De Haan,1849) and The Shrimp Metapenaeus affinis (H. Milne Edwards, 1837) from some Aquatic sites of the Basrah province

Author name: انوار مالك جبار المالكي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study intended to shed the light on some biological aspects of the invasive oriental river Shrimp Macrobrachium nipponense compared with that of Jinga shrimp Metapenaeus affinis . For this purpose, Monthly samples were collected and chosen from four Aquatic sites was chosen in the Basrah province (one at each of Al - Mashab and Al - Sallal Marshes and two at Al - Garmah River) during the period from July 2015 to June 2016. collection was done by Fishnet pulled by a boat .The Shrimp numbers were calculated during the time unit (ind./hour) .The Water’s temperature and salinity were studied, it ranged from 15 - 33 ͦ c and 2 - 9.1psu respectively. From a total of 3326, individuals were collected during the study period, only 7% were belong to the first shrimp (M. nipponense ) Compared with 93% to the second shrimp( M. affinis ).The number of each species studied shrimps, varied the monthly. The high number of M. nipponense was 38(ind./hour) recorded during July 2015 and June 2016, while the lowest was 2 (ind./hour) recorded during October and November 2015 . For M. affinis The number of individuals ranged between 980(ind./hour) during August 2015 and 29 (ind./hour) during November 2015 .The comparative monthly percentages were high during seven and six months in the marshes sites recorded for first and second shrimp respectively. The monthly variation in percentages of the two species of shrimps was studied, It ranged (for M. affinis) from 98.8% during August 2015 to 56.8% during July 2015 , while for M. nipponense , It ranged from 43.2% during July 2015 to 1.2% during August 2015 .The results of Frequency showed that the highest percent (100%) was recorded in four months for M. nipponense , these are July, October 2015 February and May 2016. While M. affinis was recorded in most months.Summary bThe population sizes for the two species were studied. For the first species( M. nipponense ), the size class 50 - 60mm dominant during all months of the study period, While for the second species (M. affinis), the size classes 40 - 50, 50 - 60, 60 - 70, and 70 - 80 were recorded during all the months for the first shrimp, the highest percent was 47.3% recorded for the size class 70 - 80 mm , While there is no individuals recorded for the size class 10 - 20 mm during all the months except July 2015 for the second shrimps ,the percentage ranged between 41.4% and less than 1% recorded during May 2016 to March 2016 respectively. On the other hand , the total Frequency for M. nipponense ranged from 83.3% to 0% for the size classes 70 - 80 mm and 10 - 20 mm respectively, While for M. affinis was from 100% recorded for the classes 40 - 50, 50 - 60, 60 - 70 and 70 - 80mm to 8.3% for the class .The Length - Length and Length - weight relationships for the two species were studied The results showed positive correlation between the total length and each of cephalothorax length and abdomen length (r= 0.932 and r= 0.945 respectively, P≤0.01 ) for M. nipponense , Also M. affinis , the correlation was positive for the total length and each of cephalothorax length(r= 0.938) and abdomen length (r= 0.971) (P≤0.01 ) . The correlation was positive between the total length and wet weight for each M. nipponense (r= 0.918 P≤0.01) and M. affinis (r= 0.913 P≤0.01).The Statistical analysis for the weight - weight relationships showed positive correlation between dry and wet weight for M. nipponense (r= 0.966, P≤0.01) and M. affinis (r= 0.918 P≤0.01).The correlation between the lengths of the body and the 2nd chela for male & female of M. nipponense (r= 0.909 and r= 0.823 respectively), these values of correlation refer to the continuous growth of the 2nd chela in male even after the adult stage .Summary cThe concentrations of ten heavy and light metals in shells & muscles of the two shrimps were studied. The results showed a significant differences in eight of them (Cadmium, Potassium, Calcium, Iron, Magnesium, Nickel, Zinc and Sodium) . While there is no significant differences recorded for Lead and Cobalt .The study of some biological aspect which concerned with distribution of eggs on pleopods of females of M. nipponense , showed that the highest mean of eggs was 547.3 (38%) carried on the 2nd pleopod while there are fewer means carried on 1st and 3rd pleopods , and no carry eggs (0%)were carried on the 5th pleopod. The Statistical analysis showed that there was significant differences between the eggs carried on 4th and 5th pleopods and each of the rest ones . lengths of pleopod was positively correlated with the number of eggs (r= 0.661, P≤0.01) The weight of ovigerous female was positively correlated with weight of eggs (r= 0.835, P≤0.01) .The combined effects of salinity and temperature on survival of newly hatched larvae (zoea1 and zoea2) of M. nipponense for 24 & 48 hours’ were tested.The highest survival percentage (100%) for zoea1 was recorded in 12 psu salinity in each of 18, 22 and 26 ͦ c ,While the lowest survival (0%) was recorded in distal water in each 30 and 34 ͦ c . the highest survival percentage was recorded in 12 psu salinity in each 18, 22, 26 and 30 ͦ c. While the lowest one recorded in distal water and 34 ͦ c. The Statistical analysis showed that the percentage survival correlates negatively with temperature and positively with salinity each alone. the combined effect of salinity and temperature on survival was positive with percentage ranged between 38 - 56 % .

عزل وتشخيص بعض الانواع الجرثومية من مياه الانهر الداخلية في مدينة البصرة ودراسة قابليتها على المعالجة الحيوية للعناصر الثقيلة == Isolation and identification bacterial species from inner rivers in Basra governorate and study their ability to Bioremediation of heavy metals

Author name: انوار عبد الوهاب مكي
Supervisor name: علي عبود شريف
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted during November and December 2013.It included studying six inner rivers branching from Shatt al - Arab river in Basrah governorate.These rivers are Abu Al - khasseb, Al - Ribat, Al - Khandaq, Al - Ashar, Al - Khura and Al - Sarraji .The study included isolation and identification of three species of genus Bacillus which are B. licheniformis,B. megaterium and B. badius ,and two species of genus Micrococcus which are M. halobius and M. kristinae .Physical and chemical characteristics of the water samples were measured, which including pH,temperature,electric conductivity and Dissolved oxygen. The results showed that there were not correlation between physical and chemical characteristics and the concentrations of the heavy metals except for water temperature which had a negative significant correlation with the concentrations of nickel and lead.The concentrations of heavy metal dissolved in water (copper, nickel , lead and cadmium ) were measured. The concentrations of Cu+2 and Pb+2 were higher than that of Ni+2 and Cd+2 in the water .However,in general the concentrations of Cu+2, Ni+2, Pb+2 and Cd+2 were high in comparison with the local and international standards.All species of bacteria showed high resistance to the heavy metals ions starting from concentration of 1 mg/l. B. licheniformis was recorded high resistance to Pb+2, Cu+2 and Ni+2 which reached 2600 mg/l, 500mg/l and 300mg/l respectively. M. halobius was also recorded a high resistance to Pb+2 and Cd+2 with 2600mg/l and 400mg/l respectively. The highest resistance was recorded by M. kristinae to nickel with 300 mg/l . The mid resistance was by B.badius to all studied heavy metals and the lowest was by B. megaterium to all heavy metals studied in comparison with the other species of bacteria.The identified bacteria were used in the process of bioremediation of the heavy metals (Cu+2, Ni+2, Pb+2, Cd+2) with different concentrations (25, 50 and100 ) mg / l. B. licheniformis showed high ability to remove Ni+2, Cu+2 and Cd+2 than other species of bacteria, which reached (28.30%, 44.34% and 24.27%) mg/l. . respectively. B. badius showed a high capability for removing Cu+2 with 45.78% mg / l .Where as B. megaterium showed a high capability for removing Pb+2 with 23.43% mg / l.. M. halobius had the highest capability for removing Cd+2 with 25.42% mg / l. and M. kristinae had a mid capability to remove the heavy metals under studied.The study included observing the effect of the incubation period on removing the heavy metals ions.The results found that the best period was 72 hours in comparison with 24 and 48 hours.Also, the study included the effect of initial concentration in the process bioremediation.The results showed that the concentration of 25mg/l was the best for removing the heavy metals ions among the other concentrations, 50 and 100 mg/l.

عزل وتشخيص احد قلويدات الايض الثانوي لبعض الطحالب ودراسة فعاليته الحيوية == Isolation and identification one alkaloid of secondary metabolites from some algae and study of biological activity

Author name: انفال نوري عباس اللفتة
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study includes an isolation, Identification and purification of three species of algae, two of them belonging to cyanobacteria they are Oscillatoria brevis and Nostoc carneum . The third one was Enteromorpha intestinalis which belong to green algae which was from different location in Basrah .There are two extract were prepared from the algal species Alcohol , and alkaloid extracts , Alcoholic extract prepared to know what was compound it had and alkaloid extracts to test bioactivity of algae Cytotoxcity also was carried out on human red blood cells, the results revealed that alkaloid extract from E.intestinalis was nontoxic, whereas bioactive compounds isolated from O.brevis , N.carneum. Showed hemolytic action .The bioactivity of alkaloid extract was examined to elucidate their on ability to inhibit the growth of gram Positive and negative bacteria. .Biological activity of alkaloid extracts of three algae isolated were determined by using the Minimal Inhibitory Concentration (MIC) againsttwo bacterial isolates. And also test the effect of filtrate pure algal culture on growth of plant pathogenic fungi ,also apparently that the algal alkaloid extract and isolated compounds exhibits antioxidant.The antitumor activity of the algal alkaloid extract from E.intestinalis against Rhabdo myosarcoma cell line was examined. The results showed that crude alkaloid extract possessed an antitumor bioactivity at low concentration 0.78mg / ml .In this study also was carried out to investigate the possibility of preventing the hypercholesterolemia by using alkaloid extract of E.intestinalis .it also aimed to study the effect of use this extract in reducing the Plasma Total Cholesterol (TC) , Triglyceride (TG), Low - Density Lipoprotein (LDL) , Very Low - Density Lipoprotein (VLDL) and no effect on High - Density Lipoprotein (HDL).The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR) and Gas Chromatography / Mass spectrum (GC - MS). The results of such analyses showed that alkaloid extract from E.intestinalisa has three compound these are Hexadecanamide , Methenamine and Benzenedicarboxylic acid, diisooctyl est and alkaloid extract from N.carneum showed that has three compound 1,2 - Benzenedicarboxylic acid diisooctyles,ا9 - Octadecenamide, (Z) - )ااااا and Didecyl phthalate .Hexadecanamide purification from alkaloid extract of E.intestinalis depended on physical and chemical properties The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR); Gas Chromatography / Mass spectrum (GC - MS) and this component showed bioactivity on gram. Positive and negative bacteria and it has also antioxidant activity

استخدام عوامل استحثاث لانتاج مؤيضات ثانوية من بعض عزلات انواع الفطر واختبار فعاليتها الضد بكتيرية والخلايا السرطانية Penicillium والكولسترول == Use of inducing agents in secondary metabolites production from some Penicillium isolates and assay their activity against bacteria , cancer cells and cholesterol

Author name: امل صالح عبد الرضا
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was designed to investigate active compounds from different Penicillium isolates ,that isolated from different sources , using different media . Eleven isolates related to five species : P. chrysogenum, P.paxilli , P. citrinum, P. cosmopolitanum, P. digitatum were isolated. After primary screening was performed , the best isolates that have high antibacterial activity against gram positive and gram negative bacterial was selected. The selected isolates were cultured on fermentation media according to the suitable media for each isolate, and the active compounds were extracted using ethyl acetate as a solvent. P. cosmopolitanum and P. chrysogenum were cultured on penicillin production medium (PPM) , P. paxilli was cultured on Aspergillus complete medium (ACM) and P. digitatum and P. citrinum were cultured on solid state fermentation media consist of wheat bran . The efficiency of fungal crude extracts from each isolate against the reference strains S. aureus NCTC 6571 and E. coli ATCC 25922 and against Candida albicans (pathogenic and reference strain)was examined, and the Minimum inhibitory concentration (MIC) was determinated for each extracts.The inhibition zone of fungal extracts against E. coli and S. aureus ranged between (20 - 50) mm , and they had high activity against C. albicans ( reference and pathogenic isolate) ranged between 25 - 45 mm.Induction of secondary metabolite production by the selected fungi was carried out using biological factor (Streptomyces sp.) , chemical agent (colchicine) and physical agent by UV radiation. Apparently that the biological induction by Streptomyces isolate was the best compared with the other treatments.The results revealed that the two fungal extracts ALO1 and Apax exhibit a high antioxidant activity at low concentrations and volumes of the extract compared to ascorbic acid as control. ALO1 had radical scavenging activity (RSA%) reach 99.9% at volume 3μl , and Apax had RSA 91.6% at concentration 500μg/ml. Also, the fungal extract ,ALO1, showed a significant activity as anticancer agent against RD - cell line. The IC50 of ALO1 was 8.5μl after 24h and 23.76μl after 72h. The hypercholesterolemia ability of ALO1 in vivo and in vitro was assayed . In vivo experiment designed using female albino mice and divided into three groups each one contain 8 mice : group 1 treated with 10μl , group 2 treated with 20μl of ALO1 , and group 3 treated with normal saline as control group. The results showed that total cholesterol , triglycerides, LDL and VLDL were significantly reduced when compared with control group while the HDL level was increased in comparing with control group. The chemical analysis of the crude extracts showed that Apen2 contained 259 μg/g of penicillin G when analyzed with HPLC apparatus . Also the GC mass analysis of ALO1 showed that Docasadionic acid initialize area76.18% and Palmitic acid initialize area 15.09% from the total identified compounds.Whereas the GC mass analysis of Apax showed that Hexandioic acid initialize area40% and Stearolic acid initialize area 35.57% from the total identified compounds.In addition , it was detected the presence of penicillin biosynthetic gene cluster : pcbAB, pcbC and penDE in P. cosmopolitanum and P. chrysogenum.The result showed that gene pcbC and penDE were found in the genomic DNA but the gene pcbAB was not amplified by PCR.

دراسة تصنيفية وبيئية لحشرات نصفية الاجنحة المائية وشبة المائية Heteroptera : Hemiptera في محافظة البصرة == Taxonomical And Ecological Study of Aquatic And Semiaquatic Insects(Heteroptera : Hemiptera) In Basrah Provience

Author name: اسماء عبد الزهرة سبع العيداني
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Present studies dealing with the taxonomical and ecological studies of the aquatic Hemiptera : Heteroptera in eight stations in Basrah provience ,which are : Al - Medina , Al - Qurna , Basrah center ,Shat Al - Arab , Al - Zubair , Abu Al - Khasib , Al - Faw and Sullein Marsh, collection was done twise monthly during the period from April 2012 to March 2013 .Results showed the presence of nine species belonging to aquatic Hemiptera , three belonging the group Gerromorpha and six the group Nepomorpha,as follows : Group : Gerromorpha(Popov,1971)Family : Gerridae ( Leach , 1815)1 - Aquarius nebularis(Schellenberg,1800)Family : Mesoveliidae(Douglast and Scott , 1867 )2 - Mesovelia vettigera( Horvath , 1895 ) ( apterous )M .vettigera( Horvath , 1895 ) ( macropterous )Family : Macroveliidae3 - Macrovelia hornii(Uhler , 1872 )Grouop : Nepomorpha(Popov,1968)Family : Nepidae ( Latreille , 1802 )4 - Nepa cinerea( Linnaeus , 1758 )Family : Corixidae ( Leach,1815 )Subfamily : Cymatianiae ( Walton , 1940 )5 - Cymatia bonsdorffii(Sahlberg , 1819 )Subfamily : Corixinae ( Leach , 1815 )6 - Sigara(Vermicorixa) latelaris ( Leach , 1815 )Family : Belostomatidae ( Leach , 1815 )7 - Lethocerus fakir(Mayr , 1852)Family : Notonectidae ( Latreille , 1802 )8 - Anisops sardeus(Herrich - Schaeffer , 1849)AbstractBFamily : Pleidae ( Fieber , 1851 )9 - Plea leachi(McGregor and Kirdaldy,1899)Among the collected species ,six were regarded as new records to the Iraqi fauna of this order,they were : A. nebularis,M. vettigera( macrpterous) , M . vettigera( apterous), Ma.hornii , N.cinerea , C.bonsdorffii andP.leachi.Study also deal with the measuring of some ecological aspects like air and water temperatures , salinity , pH and dissolved oxygen ,and their effected on the monthly distribution of the aquatic Hemiptera. Temperature was the most effective aspect in the monthly distribution of the insects ,as the lowest numbers were recorded in Summer months and the highest number were in Winter months , the ranges of the air temperatures were 9 - 43º C and water temperatures were 2 - 33º C .The highest concentrations of salinity was recorded in Al - Zubair , Abu Al - Khasib and Al - Faw reached to 35g/l in Al - Zubair in July , the pH ranges were the same in all stations and was alkalid , their ranges were 7.3 - 7.8 , about the dissolved oxygen it was low and same in all stations except in Sullein Marsh which reached to 11 mg/l in January .The concentration of some heavy metals in water also studied like Fe, Pb, Cu and Zn , the highest concentrations were to Fe and Zn in Basrah center and Al - Zubair, and the highest concentration for Fe in Al - Zubair reached to 6362.21μ/L in August, while highest concentration for Zn in Basrah center reached to 1365.92μ/L in July, while in the other stations the concentrations were lowest .The study also used the water boatmen Sigara latelaris(Leach,1817) as bioindicators of heavy metals pollution in the water of Basrah provience , and taken Sullein marsh as control , and measuring the concentrations of the heavy metals in insects tissues , water and sediments , the results showed that the insects collected from the water of the center of the city can accumulate Fe andAbstractCZn in their tissues and the highest concentrations reached to 3562.16 and 3778.12 μ /gm for Fe and Zn in July , while in the insects taken from Sullein marsh were 286.25 and 285.17 μ /gm .The concentrations of heavy metals in water and sediments of the city center were measured and the highest concentration was for Fe and Zn , and reached to 1569.91 and 1365.91 μ /L of the water of city center , while reached to 215.01 and 223.14 from the same metals in the water of Sullein marsh , the concentrations of the same metals were recorded in sediments of the city center and reached to 2865.16 and 1982.56 μ /gm for Fe and Zn , and the highest concentrations of the same metals in Sullein marsh were 418.57 and 393.74 μ /gm .

دراسة تاثيرالسمية النباتية والوراثية لتراكيز مختلفة من الاوكسينات ومضادات التلوث في استحثاث الكالس الاولي لنخيل صنف الحلاوي. (Phoenix dactylifera L.) التم == Assessment of Phytotoxicity and Genotoxicity of different Auxins concentrations and decontamination agents on initiation of date palm (Phoenix dactylifera L.) primary callus of Hillawii cv.

Author name: اسراء عبد الرزاق حميد السامر
Supervisor name: صبيح داود محمد العطبي | محمد حمزة عباس
General topic: Biology
Specific topic: Plant - Tissue
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted to evaluate the effect of different concentrations of the growth regulators 2, 4 - D; Dicamba and NAA on the induction of date palm (Phoenix dactylifera L.) primary callus Hillawii cv., by analyzing morphological, biochemical; anatomical and molecular responses. Three concentration of 2, 4 - D (10; 50 and 100 mg/L), Dicamba (1; 5 and 10 mg/L) and 30 mg/L of NAA were examined during the course of this study. in addition, several antimicrobial agents were selected to evaluate their morphological and biochemical impacts on the primary callus of date palm including the antibiotics (Gentamycin 50 mg/L and Chloramphenicol 50 and 100 mg/L), as well as, the fungicides (Switch 1 g/L and Beltanol 1 ml/L), After induction of the primary callus from each treatments, the following results were obtained : 1. The results showed that the treatments with 2, 4 - D at 10 mg/L and Dicamba at 1 and 5 mg/L did not stimulate any callus growth over the time of incubation on MS medium.2. It was evident that the treatments of different growth regulators have a significant effect on the initial period (day) for callus initiation, the shortest period have been obtained with the low concentration of 2, 4 - D (50 mg/L) and Dicamba (10 mg/L) which were 67.57 and 66.75 days, respectively, while the high concentration of 2, 4 - D (100 mg/L) led to increase this period significantly up to 120 days.3. Both fresh and dry weight of date palm primary callus decreased significantly at the treatment of high concentration of 2, 4 - D (100 mg/L) up to two folds compared to the treatment at low concentration of 2, 4 - D (50 mg/L), similar trend of results were found with browning percentage and intensity.4. Biochemical analysis of produced date palm primary callus revealed that the high concentration of 2, 4 - D (100 mg/L) led to a significant increase in the total content of carbohydrates; Proline, Peroxidase activity and Phenolic compounds which considered as a stress indicators, and another decrease of total soluble proteins and free amino acids was accompanied with this treatment compared to the same growth regulator at low concentration and Dicamba treatments.5. Anatomical study revealed that asignificant damage was caused by the high concentration of 2, 4 - D (100 mg/L) on date palm primary callus, this damage was evident from the abundant and distribution of tannins compounds across the primary callus tissues, thus, was accompanied with high percent of browning, compared with the treatments of 2, 4 - D at low concentration, Dicamba and NAA.6. The analysis of Protein profile revealed the similarity between the patterns of control treatment (juvenile leaf of Hillawii cv.) and 2, 4 - D at low concentration, as well as, Dicamba. Similarity indices results for protein profile of date palm primary callus proved the genetic similarity of 100% between control treatment and both 2, 4 - D (50 mg/L) and Dicamba ones, while, the lowest similarity percentage was observed with high concentration of 2, 4 - D (100 mg/L).7. The results of RAPD marker analysis of the extracted genomic DNA form date palm primary callus using four deca oligonucleotide primers showed the efficiency of three primers (OPAR3; OPAR8 and P.650) in their amplification of examined DNA,. RAPD profile revealed an identical matching between control treatment and 2, 4 - D at low concentration, followed by Dicamba and NAA auxins, while, the increasing in 2, 4 - D concentration up to 100 mg/L led to disappearance and the appearance of new DNA bands compared to control treatment, with a highest genetic distance among other treatments.Similarity coefficient analysis showed that the highest genetic similarities were found between control treatment and 2, 4 - D (50 mg/L) with similarity index of 96% , followed by Dicamba and NAA treatments with indices of 74 and 66%, respectively, the lowest similarity index was obtained between control and 2, 4 - D at high concentration which was 27%.8. The screening results of antimicrobial agents showed that the treatment with Switch fungicide (1 g/L) and Gentamycin antibiotic ( 50 mg/L) did not stimulate any callus growth over the time of incubation on MS medium supplemented with either 2, 4 - D or Dicamba.9. Beltanol fungicides (1 ml/L) treatment had a negative effect on the initiated primary callus either by 2, 4 - D or Dicamba, its treatment led to a high significant reduction in total solubleprotein and free amino acid content compared to control results, while an increase of total carbohydrates, Phenolic compounds, Proline and Peroxidase activity was detected with Beltanol fungicide.10. The Chloramphenicol treatment at low concentration (50 mg/L) showed positive results on the growth of primary callus at 2, 4 - D or Dicamba auxins according to morphological and biochemical analysis.

عزل وتشخيص بعض انواع البكتريا المكسرة للهيدروكاربونات النفطية الاليفاتية في الترسبات النفطية لمحافظة البصرة == Isolation and Identification of some species of degradable Bacteria of N - Alkanes hydrocarbons compounds in oil sludge at Basrah Governorate

Author name: استبرق عبد الحسين سلمان جدي البوغازي
Supervisor name: ميثم ايوب عبد القادر الحمداني
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:
Abstract: In the oil industries, large amounts of oil sludge are generated through the refining of crude oil. The main reason for its composition that is when exporting crude oil various kinds of oils (heavy and light ones) are mixed together for the purpose of exportation. But , the mixing process between different types cannot be fully , due to the lack of homogeneity among different quality of crude oil . So, the heavy parts of oil are precipitated to the bottom of tank and with passage of time they accumulate to be sediments that’s known as oil sludge stated at the bottom of the oil storage tank in the cleaning process , oil tanks are emptied and discharged in some spaces or reservoir (pits) located near the tanks called "oil pits". Which are places of dump excess oil of various types of oil from different oil tanks or they are close places from oil / water separating unites .From these spots, samples of two kinds of oil sludge : the bottom of oil tank (during the maintenance of the tank) and oil pits (near the unities of separating water from oil) are collected. Also, samples of soil surrounding the oil pits from five oil locations in Basrah city are collected too. Those locations are : west of Gurna, Northern Rumela, Southern Rumela, Nahar Umar, Alzubeir.At the rate of redundantly for each location and for each type of the study samples.This investigation is considered as the first which conducted in Iraq which include firstly the isolation and identification different types of bacterial species from different oil sludge, and secondly use some of them in biodegradation studies for two kinds of carbon sources of oil sludge : (oil sludge of tank bottom , oil sludge of oil pits) .Oil degrading bacterial species have been isolated on a selective mineral salt medium Bushnell and Hass Mineral Salt Medium (BHMS), supplemented with a carbon source. They are also identified depending on the Morphology and biochemical characteristics; four genus of bacteria (out of 89 bacterial isolates of oil sludge from the tank bottom)Bare identified. They include : 32 Pseudomonas spp. , 27 Rhodococcus spp. 21 Bacillus spp. , 9 Arthrobacter spp. , Also four genera of bacteria (out of 101bacterial isolates of oil sludge from the oil pits) are identified. They include : 41 Pseudomonas spp. 27 Rhodococcus spp. , 22 Bacillus spp. 11 Arthrobacter spp. , , While 8 species and 3 genus of bacteria (out of 259 bacterial isolates of the soil surrounding the oil pits) are identified. They include : 41 Aeromonas spp., 39 Corynebacterium spp., 39 Pseudomonas aeruginosa, 36 Bacillus spp., 33 Micrococcus leteuc, 23 Enterobacter intermedius, 22 Pseudomonas fluorescens, 12 Micrococcus varians 7 Staphylococcus epidermidis, 4 Staphylococcus saprophyticus,3Staphylococcus aureus .The biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes and two compounds of Pristane and Phytane are measured. Also, the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes and the two compounds of Pristane and Phytane are measured for each four genus which were isolated from the oil sludge pits, and it is also done for one isolated genus from the soil surrounding the oil pits. The measuring process was fulfilled as a comparative study with the control flasks. Thus, the oil sludge (tank bottom ) was used as a source of carbone in the first experiment While, the oil sludge (oil pits) was used as a source of carbone in the second experiment . The incubate time of the experiment is 27 days during which a lost of the percentage of hydrocarbon compounds each 9 days are recorded by using( Capillary Gas Chromatography ). The results showed the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard toCbacteria 73.8 % Pseudomonas spp., 70.3 % Rhodococcus spp. 65.7 % Arthobacter spp., 55.6 % Aeromonas spp.,51.5 % Bacillus spp., Whereas, the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria 80 % Pseudomonas spp., 74.9 % Aeromonas spp., 70.4 % Rhodococcus spp., 64.8 % Arthobacter spp.,55 % Bacillus spp., While , The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard to bacteria 73.7 % Pseudomonas spp.,70.3 % Rhodococcus spp. 65.6 % Arthobacter spp., 55.3 % Aeromonas spp. ,51.2 % Bacillus spp., Whereas, The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria79.2 % Pseudomonas spp., 73.8 % Aeromonas spp., 69.2 % Rhodococcus spp.,63.7 % Arthobacter spp., 53.7 % Bacillus spp. It was found that there is approximately match between the biodegradation of mole percentage by using the area and those by using the concentration for each component .

التحلل والمعالجة الاحيائية للمركبات الهيدروكاربونية النفطية بوساطة سلالات Streptomyces وNocardiopsis المعزولة من الترب الملوثة بالنفط في محافظة البصرة - العراق == Biodegradation and bioremediation of petroleum hydrocarbons by Streptomyces and Nocardiopsis strains isolated from oil contaminated soil of Basrah Governorate - Iraq

Author name: احمد عبد برغال الاسدي
Supervisor name: كوثر هواز مهدي | نادية عبد الامير المظفر
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: سبعة سلالات من البكتريا الخيطية تعود الى جنسي Streptomyces وNocardiopsis عزلت من ترب شركة مصافي الجنوب الملوثة بالنفط الخام في البصرة جنوب العراق، وقد تم اختبار قدرتها على النمو بوجود النفط الخام باستعمال وسط ملحي سائل مزود بالنفط الخام كمصدر وحيد للكربون. شملت السلالات على S. variabilis 7525 وS. variabilis SW75 وS. cellulosae XFB - T وS. parvus S86 وS. bacillaris S4BW2 وS. flavoviridis VITHM - 1 وN. synnemataformans ITD - 3 بنسبة تشابه من 99٪ الى 100٪. في هذه الدراسة تم العثور على سلالات جديدة من البكتريا الخيطية المعزولة من التربة الملوثة شخصت باستعمال تحديد تتابعات جين الحامض النووي الريبوزي 16S rRNA وقد اظهرت السلالات قدرات مختلفة على تحلل النفط الخام في الوسط الملحي السائل من 50.2٪ الى 72.4٪. كما تم تقييم تاثير الظروف الزرعية من درجة الحرارة والدالة الحامضية وتركيز النفط الخام والعناصر النزرة في نمو سلالات البكتريا الخيطية وتحلل النفط الخام، وقد لوحظ ان اعلى نمو للخلايا واعلى تحلل للنفط الخام كان 80٪ في ظروف زراعة مثالية ( درجة حرارة 30 ᵒم ودالة حامضية 7 بعد 10 ايام من الحضن وزادت هذه النسبة الى 85٪ من قبل السلالة S. cellulosae XFB - T بعد اضافة العناصر النزرة.في حين اظهرت نتائج اختبار التحلل الحيوي للنفط الخام في التربة من قبل سلالات البكتريا الخيطية منفردة بعد 60 يوما من الحضن تحت ظروف مختلفة ، اظهرت ان اعلى قيمة للتحلل كانت 87 ٪ من قبل النوع S. bacillaris عند درجة حرارة 30 ᵒم وحجم لقاح مضاف 10مل/ 25غم تربة ومستوى رطوبة 40٪.المعالجة الاحيائية هي ستراتيجية فعالة لتنظيف المواقع الملوثة بالهيدروكربونات. ونتيجة تاثر الكائنات الحية الدقيقة من تلك التربة يحدث لها اضطراب وبالتالي يتم انتقاء الاحياء المجهرية القادرة على استهلاك الهيدروكربونات لاستخدامها في هذه التقنية الفعالة في التعامل مع الملوثات الهيدروكربونية. الهيدروكربونات التي تتفاعل مع نسجة التربة والكائنات الحية الدقيقة هي التي تحدد مصير الملوثات وطبيعتها الكيميائية والقدرات الهادمة للميكروبات. الاثار المحتملة لنشارة الخشب، وخليط من روث الابقار والغنم وتعديل المكملات الغذائية لتحفيز الكائنات الدقيقة الاصلية وزيادة المعالجة البيولوجية للهيدروكربونات، هذه العملية تم اجراءها باستعمال طريقة المعالجة خارج الموقع بطريقتي الكومة والمفاعل الحيوي، بخلط التربة الملوثة مع نشارة الخشب والروث الحيواني ودعم التربة بالمغذيات والعناصر النزره مع توفير الرطوبة الملائمة والظروف المواتية لنمو الكائنات الحية الدقيقة كما قدمت التهوية الصناعية للتربة في الكومة والمفاعل بوساطة شبكة من الانابيب المثقبة لمحاكاة المعالجة الحيوية التي استمرت مدة 90 يوما.خلال تلك الفترة، رصدت الهيدروكربونات النفطية الكلية (TPHs) وازالة الالكانات الاعتيادية والتغيرات في المجتمعات البكتيرية. وقد اظهرت النتائج ان (TPHs) قد انخفضت من 52غم/كغم الى 10.6 غم / كغم بمعدل تحلل بلغ 79.6٪ ومعدل تحفيز كان 71.4٪ في تجربة الكومة ومن 52غم/كغم الى 13.5 غم / كغم بمعدل تحلل بلغ 74٪ ومعدل تحفيز كان 63.5٪ في تجربة المفاعل الحيوي. وخلال مدة 45 يوما من الاختبار بلغت اعداد الكائنات الحية الدقيقة من البكتيريا المضافة والمستوطنة التي تحلل المواد الهيدروكربونية اعلى مستوى لها 2 × 710 خلية/ غم و1.1 × 710 خلية/ غم في الكومة والمفاعل الحيوي على التوالي.بناء على هذه المعطيات، نستنتج ان المعالجة الحيوية خارج الموقع افضل ستراتيجيه غير مكلفة وفعالة وصديقة للبيئة وبالتالي قد توفر خيارا قابلا للتطبيق لمعالجة التربة من الملوثات الهيدروكربونات النفطية. | Seven actinomycetes strains from genera Streptomyces and Nocardiopsis isolated from hydrocarbon contaminated soil in Basra Governorate, Iraq were selected for their capacity to grow in the presence of crude oil. Their growth rates and biodegradation ability were investigated in mineral salt media supplemented with light Remella crude oil. These strains consist of Streptomyces variabilis 7525, Streptomyces variabilis 5W75, Streptomyces cellulosae XFB - T, Streptomyces parvus S86, Streptomyces bacillaris S4BW2, Streptomyces flavoviridis VITHM - 1 and Nocardiopsis synnemataformans ITD - 3, that the sequence identity range from 99% to 100%. Our study find a new actinomycetes strains isolated from contaminated soil. these strains appears different capacity on the degradation of crude oil in mineral salt media , the highest was found to vary from 50.2% to 72.4%.The effect of the cultivation factors (temperature, pH, and concentration of crude oil and trace elements ) on growth of the actinomycetes strains and crude oil degradation was evaluated. The highest cell growth and the amount of crude oil degraded 80% were observed in optimized cultivation conditions (30oC and initial pH 7) after 10 days, this ratio reached to 85% by addition of trace elements solution at same condition by S. cellulosae XFB - T.The biodegradation test lasted 60 days in soil by actinomycetes strains at different factors shown the highest of the crude oil was removed in experiment that inoculums with 10ml of each bacterial strain, value 87% by Streptomyces bacillaris S4BW2 at 30oC. Bioremediation is an efficient strategy for cleaning up sites contaminated by hydrocarbons. Microorganism of soil is affected by hydrocarbon disturbance thus selective enrichment of hydrocarbon utilizers occurs. The technology is very effective in dealing with petroleum hydrocarbon contamination. Hydrocarbons react with the soil matrix and microorganisms determining the fate of the contaminants relative to their chemical nature and microbial degradative capabilities. The potential effects of sawdust, and mixture of cow and sheep dung as amendment nutrient supplements to biostimulate autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile and bioreactor. The soil was ground and fluffed by admixture of 1.5% sawdust, then supplemented with the necessary minerals and watered to provide conditions favoring microorganism growth industrial aeration was provided in pile by system of a abundant perforated drainage - pip network to simulate bioremediation treatments through a 90 - day period. During this period, we monitored total petroleum hydrocarbons (TPHs) and n - alkanes degradation and changes in bacterial communities. The (TPHs) had been reduced from 52 to 10.6 g/kg (79.6 %), the rate of Biostimulants Efficiency (BE) was (71.4%) in biopile and from 52 to 13.5 g/kg (63.5 %) in bioreactor . In soil, the dominant microorganism population comprised Gram - positive bacteria from actinomycete group and autochthonous microorganisms which decompose hydrocarbons reached highest level 2 x 107 and to 1.1 x 107 CFU/g at 45 days in biopile and bioreactor respectively . Based on these data, the study conclude that is ex situ ( Biopile and bioreactor ) experiment the best strategy, inexpensive, efficient, and environmentally friendly and may thus offer a viable choice for petroleum hydrocarbons - contaminated soil remediation.

دراسة للطفيليات المعوية المعزولة من الخضروات المطرية من بعض اسواق مدينة بغداد

Author name: وسن عداي مهدي المرسوي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:
Abstract: A study was carried out during the period from November 2010 to October 2011 to detect the intestinal parasites which contaminate the common fresh vegetables. A total of 303 samples were taken from three wholesale markets and three retail markets, in Baghdad province. Ten kinds common vegetables were selected. These vegetables were Celery (Apium graveolens); Parsely (Patroselinum crispum); Garden cress (Lepidium sativum); Leek (Allium porrum); Radish (Raphanus sativus); Lettuce (Lactuca sativa); Spinach (Ipomoea aquatica); Beet (Beta vulgaris); Mint(Mentha spicata) and Cabbage (Brassica oleracea).Sedimentation and floatation techniques were used for samples examination. Three different solutions (ZnSO4, saturated NaCl , saturated sucrose ) were used in floatation technique. The present study has been shown that the collected samples of vegetables were contaminated with sixteen species of intestinal parasites. It was found that the percentages of contamination with these parasites were as following : (Giardia lamblia) (29.4%), Entamoeba histolytica(24.5%), Entamoeba coli(16.9%), Balantidium coli (8%), coccidia(9.9%) and Blastocystis hominis(0.7%). For intestinal helminthes Ascaris lumbricoides (15.6%), Toxocara spp.(10.3%), Fasciola spp. (5.3%), Schistosoma haematobium (1.7%), Schistosoma masnsoni (1.4%), Taenia spp. (4.7%), Diphyllidum caninum (3.9%), Trichuris trichiura (3.3%), Enterobius vermicularis (6.9%) and Nematoda larvae (6.3%).The present study revealed that the percentage of contaminated vegetables by using sedimentation technique (72.3%) was significantly higher than that of flotation techniques (53.1%). Additionally it was found that, the different kinds of vegetables showed a significant difference in the percentages of contamination with the parasites in both techniques. Thus, the percentage of contaminated Garden cress was (93.5%), (72.4%) in both sedimentation and flotation technique respectively, while Cabbage showed (50%), (36.7%) respectively.A significant high percentage of contamination was recorded in Al - Nahrin wholesale market (89.2%) in comparison with low percentage (59.4%) in Al - Rasheed wholesale market. Additionally, it was found that the percentages of contaminated vegetables in wholesale (76.26%) were significantly higher from that of retail market (64.76%).The present study, also, showed that the percentage of detection of parasites by using ZnSo4 solution (31.2%) was more efficient than that showed by saturated sucrose (8.9%). Additionally; the soaking with normal saline (47.83%) showed a higher percentage of contamination than that by using distilled water (17.39%).The percentages of seasonal contamination were varied significantly, therefore, it was found that spring showed the highest percentage (29.7%) while summer the lowest (20.9%). The washed samples of Garden cress which obtained from the three wholesale markets, reduced the percentages of contamination with parasites but did not remove the parasites completely. Therefore, a significant difference has been noticed between washed (56.76%) and unwashed samples (76.66%).Concerning, the percentages of infections with intestinal parasites, it was found that the medical center (14 July) showed (29.4%) while Al - Ameria center (19.2%). Furthermore, the percentage of parasites infections was significantly higher in the summer (46%) than in winter (12.67%).The present study indicated that there were a positive coordination between the high percentages of contaminated vegetables and the high percentages of infection in the medical centers as in the case of G.lamblia and E.histolytica.Finally, it could be conducted that the contaminated vegetables played an important role in transmission of intestinal parasites to human, particularly, when these vegetables were eaten fresh without sterilization or good washing.

دور بعض اجناس الساينو بكتريا في التحلل الحيوي لبعض المركبات النفطية

Author name: محمود خلف صالح الجبوري
Supervisor name: علي صالح حسين الجبوري | اياد محمد علي فاضل العبيدي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة عزل وتشخيص ثلاثة اجناس من السيانوبكتريا من بيئات التربة والمياه العذبة في محاولة لمعرفة دور هذه الاحياء في عملية التحلل الحيوي للمركبات الهيدروكاربونية وهذه الاجناس هيNostoc sp. , Anabaena sp. ,Microcyst sp. واستخدم نوعان من المركبات الهيدروكاربونية هي نفط كركوك الخام وزيت الغاز.بينت النتائج ان لجميع عزلات السيانوبكتريا المختبرة القدرة على استحلاب واسع للمركبات الهيدروكاربونية في النسب 2%,1%,0.5% في العزلات الحاوية على الحويصلة المغايرة ، بينما كانت النسب 1%,0.5% هي اكثر استحلابا في الجنس Microcyst sp. غير الحاوي على الحويصلة . في حين لم تكن العزلات ذات قدرة عالية على الاستحلاب في التركيز5% . وقد اظهرت التحاليل المجراة باستخدام جهاز طيف الاشعة تحت الحمراء تغيرا واضحا في تركيب هذه المركبات النفطية.كما تناولت الدراسة تاثير هذه المركبات في بعض الفعاليات الفسلجية للسيانوبكتريا مثل كمية الكلوروفيل(A) وبعض التغيرات المظهرية والخلوية في السيانوبكتريا مثل تكرار الحويصلات المغايرة ، وقد بينت النتائج ان المركبات النفطية ادت الى اختزال كبير في نسبة الكلوروفيل (A) حيث كان التناسب عكسيا بين نسبة الكلوروفيل وتركيز المركب الهيدروكاربوني . كما ادت المركبات الهيدروكاربونية الى زيادة كبيرة في عدد الحويصلات المغايرة ، وان النسب 1%,0.5% من هذه المركبات كانت الاكثر تاثيرا في زيادة عدد الحويصلات .كما تناولـت الدراسة اجراء اختبار البقائية للـسيانوبكتـريا في التراكيــز 80,60,40,20 ) % ) من النفط الخام وزيت الغاز وقد لوحظ ان بقائيـة الاجناس في زيت الغاز كانت اعلى منها في النفط الخام مما يدل على ان تاثير زيت الغاز كان اقل من تاثير النفط الخام .

الخصائص البيئية لمياه الصرف في معمل دباغة بغداد - العراق

Author name: علياء سلمان مهدي
Supervisor name: ميسون مهدي صالح الطائي | مجيد حسن
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Babylon - College Of Education For Pure Sciences
Language: Arabic
University location: Babylon
First pages:

دراسة تشريحية ونسجية وكيمونسجية مقارنة للغدة الدرقية وجار الدرقية في نوعين من الفقريات العراقية (بيز ابن عرس، Herpestes javanecus والقنفذ طويل الاذن ، Hemiechinus auritus) باستعمال الواسم Anti - Calcitonin, code 140778) ) == Study of Thyroid and Parathyroid Gland in Two Iraqi Vertebrates ( Weasl , Herpestes javanicus and Long - Eared Hedgehog , Hemiechinus auritus) Using Marker (Anti - Calcitonin, code 140778)

Author name: رنا علاء عبد الحميد هايس العامري
Supervisor name: حسين عبد المنعم داود
General topic: Biology
Specific topic: Zoology - Anatomy
Degree: Doctorate
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:
Abstract: هدفت الدراسة الحالية التعرف على الوصف التشريحي والتركيب النسجي والكيمو نسجي - المناعي للغدتين الدرقية وجار الدرقية لنوعين من اللبائن العراقية ممثلة ب بيز ابن عرس ( ( Herpestes javanicus والقنفذ طويل الاذن ( Hemiechinus auritus ) وقد استعملت تقنيات مختلفة لانجاز الدراسة .• الغدة الدرقية Thyroid Glandاظهر الفحص التشريحي للغدة الدرقية في حيوان بيز ابن عرس (H. javanicus) البالغ (ذكور, اناث) ان الغدة تتكون من فصين (ايمن, ايسر) وتاخذ موقعا في منطقة العنق اسفل الحنجرة. ويتموضع فصا الغدة على جانبي الرغامى عند الحلقات (5 - 1) من حلقات الرغامى ولم يلاحظ وجود تركيب البرزخ (Isthmus) الذي يربط بين فصا الغدة , ويتباين لون الغدة بين البني والاحمر وفي الغالب تظهر بلون بني محمر(Reddish brown) يظهر فصي الغدة في شكل بيضوي او اهليجي, اظهرت نتائج الدراسة الحالية في حيوان القنفذ ( H. auritu ) تماثلا واضحا مع حيوان بيز ابن عرس فيما يخص الوصف التشريحي باستثناء كون فصا الغدة بيضويتي الشكل.تحاط الغدة في حيوان بيز ابن عرس بمحفظة من نسيج ضام (Connective tissue) تتكون من طبقتين, طبقة خارجية External layer)) تتالف من الياف مغراوية (Collagenous fibers) وقليل من الياف مرنة ((Elastic fibers ويتخللها ارومات ليفية Fibroblast)), وطبقة داخلية ((Internal layer تتالف من الياف مغراوية يتخللها الياف عضلية ملساء Smooth muscle fibers) ) ,كما تتضمن المحفظة اوعية دموية ((Blood vessels واعصاب (Nerves), تمتد من المحفظة الداخلية حويجزات رقيقة تقسم الغدة الى فصيصات غير مكتملة . اظهرت المحفظة في حيوان القنفذ تماثلا مع المحفظة في حيوان بيز ابن عرس.يتكون نسيج الغدة الدرقية في حيوان بيز ابن عرس بصورة رئيسية من الجريبات (Follicles) والخلايا الجنيب جريبية (Parafollicular cells) واوعية دموية واعصاب. تتخذ الجريبات اشكالا متعددة منها بيضوي (Oval), ومنها دائري ((Rounded, واحيانا تظهر بشكل غير منتظم (Irregular shape), وتكون الجريبات باحجام مختلفة صغيرة, متوسطة وكبيرة . اظهر القنفذ تماثلا مع حيوان بيز ابن عرس فيما يخص حجم وشكل الجريبات. تبطن الجريبات في بيز بن عرس اما بنسيج ظهاري مكعبي واطئ بسيط ( Low simple cuboidal epithelial tissue) او نسيج ظهاري مكعبي بسيط (Simple cuboidal epithelial tissue) , بينما في حيوان القنفذ يظهر نسيج ظهاري عمودي بسيط (Simple columnar epithelial tissue) فضلا عن النسيج الظهاري المكعبي الواطئ البسيط والظهاري المكعبي البسيط . تحتوي تجاويف الجريبات على مادة جيلاتينية هي الغروان Colloid)) , وتظهر الجريبات تباينا في كمية الغروان اذ ان بعض الجريبات تكون مملؤة بالغروان والبعض الاخر يحتوي على كمية قليلة والقليل منها يظهر خاليا تماما من الغروان في كلا الحيوانين موضوع الدراسة, تنتشر في نسيج الغدة خلايا تدعى بالخلايا الجنيب جريبية (Parafollicular cells) او تسمى خلايا الكالسيتونين (Calcitonin cells) او (C - cells), وتظهر هذه الخلايا باشكال متباينة يتراوح بين الدائرية (Rounded) الى مضلعة الشكل ( (Polygonal shape, وهي تظهر اما بشكل مفرد (Single) او بشكل تجمعات (Clusters) في نسيج الغدة, وتحتل هذه الخلايا موقعين اساسيين في نسيج الغدة , الاول داخل جريبي Intrafollicular position) ) وتكون بين الغشاء القاعدي والخلايا الظهارية الجريبية والثاني بين جريبي Interfollicular position)) وتكون بين جريبات الغدة الدرقية , وتكون قليلة العدد ولم تظهر اختلافا في حيوان بيز ابن عرس عن القنفذ .اظهر التفاعل الكيمونسجي المناعي في حيوان بيز ابن عرس والقنفذ استجابة واضحة للتفاعل المناعي مع الاجسام المضادة للكالسيتونين, وظهرت الخلايا متقبلة للون البني وهو دليل التفاعل المناعي .• الغدة جار الدرقية Parathyroid Gland توجد الغدة جار الدرقية بتماس مع الغدة الدرقية وتحاط بمحفظة من نسيج ضام وهي امتداد لمحفظة الغدة الدرقية والتي تتكون من الياف مغراوية والياف مرنة ((Elastic fibers والياف عضلية ملساء واوعية دموية واعصاب . توجد الغدة جار الدرقية في موقعين طرفي قمي وطرفي جانبي. تمتد من محفظة الغدة جار الدرقية حويجزات الى داخل الغدة لتفصلها الى فصيصات غير كاملة، وتنتشر داخل نسيج الغدة. اما في القنفذ ( H.auritus ) فانها تقع ضمن نسيج الغدة الدرقية وتظهر بشكل فصوص (4 - 2) فصوص دائرية او بيضوية الشكل محاطة بمحفظة رقيقة تفصلها عن جريبات الغدة الدرقية.تم تمييز ثلاثة انواع من الخلايا في نسيج الغدة جار الدرقية في حيوان بيز ابن عرس اذ تمثلت الخلايا الاكثر انتشارا بالخلايا الرئيسة ((Chief cells، والخلايا الحمضة (Oxyphil cells) وخلايا الماء الشفافة ( (Water - clear cellsوهي تماثل الخلايا في نسيج القنفذ وتترتب بشكل حبال خلوية ضمن نسيج الغدة.اظهرت الغدة جار الدرقية في بيز ابن عرس استجابة للتفاعل الكيمو نسجي المناعي, اذ ظهرت خلايا الغدة متقبلة للون البني دليل التفاعل المناعي اذ تظهر الاستجابة المناعية للخلايا على مستوى السايتوبلازم والغشاء البلازمي في الخلايا الرئيسة ( (Chief cells, واظهرت الاستجابة المناعية في الغدة للقنفذ اختلافا بالنسبة لشدة استجابة الخلايا حيث كانت اقل من حيوان بيز ابن عرس .اوضحت نتائج الدراسة الحالية الخاصة بتحليل الاحماض الامينية في مستخلص الغدة الدرقية وجار الدرقية في حيوان بيز ابن عرس (H.javanicus ) (ذكور، اناث), تشخيص (13) حامضا امينيا في الذكور و(11) حامضا امينيا في الاناث, اما في حيوان القنفذ H.auritus (الذكور، الاناث), فقد تم تشخيص (12) حامضا امينيا في كل من الذكور والاناث. | Anatomical examination of thyroid gland in adult (males &females) H. javanicus revealed that the gland consist of two lobes (right & left), situated below the layrnx in neck region. The two lobes located at the sides of trachea (1 - 5) rings of trachea. they appear without Isthemus. The colour of the gland is reddish brown. The shape of the lobes either to be oval or elliptical. On the other hand the thyroid gland in H. auritus showed a clear similarity with that of H. javanicus with the exception of the oval shape of the gland. Thyroid gland of H. javanicus surrounded by capsule of connective tissue consist of external layer of collagenous and elastic fibers, fibroblast and internal layer consist of collagenous fibers with smooth muscle fibers, the capsule also contain blood vessels and nerves. Thin trabeculae extend from the capsule divided the gland into incomplete lobules. The capsule of thyroid gland in H. auritus showed clear similarity with that of H. javanicus with the exception of the adipose tissue between the capsule and gland tissue in H. auritus.Histologically thyroid gland in H. javanicus consists of follicles, parafollicular cells, blood vessels and nerve. The follicles appear in different shapes (oval, rounded and irregular shapes, and have different size (small, medium and large). The thyroid gland tissue in H. auritus has the same appearance of that in H. javanicus. The follicules in H. javanicus lined by epithelial tissue either to be Lwo simple cuboidal or simple cuboidal epithelium. In H. auritus lind by Lwo simple cuboidal, simple cuboidal or even simple columnar epithelial tissue. The follicular cavities contain different quantities of colloid, and few of them lack of collid in both under investigated animals. Parafollicular cells are spreading in gland tissue; they also called calcitonin cella or c - cells. These cells have different shapes either to be rounded or polygonal shape, and found as single or clusters. They are either to be interfollicular or intrafollicular position. The immunohistochemistry reaction revealed clear positive reaction for calcitonin , and the appearance of brown colour represented evidence for the immune reaction .• Parathyroid glandResults of the present study revealed that parathyroid gland situated closely with thyroid gland in both animals under investigatin . The gland surrounded by a capsule of connective tissue represented as an extension from the capsule , which consist of collagenous fiber, elastic fibers, smooth muscles fibers and blood vessels and nerves. The parathyroid gland situated at two position (lateral terminal / apical terminal) the trabeculae wich exted from the gland capsule to the gland tissue separated the gland into incomplete. In H. auritus the parathyroid gland formed from (2 - 4) lobes with rounded or oval shapes and surrounded by thin capsule separated from the thyroid gland follicles.Three types of cells have been differentiated in the parathyroid tissue in H. javanicus represented by chief cells wich apper the most abundant in comparison with the other two types, the second type are the oxyphil cells and the third are water - clear cells, they are similar in both animals and appear as a cellular cords within the gland tissue. The immunohistochemical study in parathyroid gland of H. javanicus reveald positive reaction for calcitoninin, where ̛s the cells appear ̛s in brown color giving the evidence for the immune reaction in cytoplasm and plasma membrane of the chief cells In H. auritus there was clear immune reaction but less than H. javanicus . Result of the present study related with the amino acid analysis of the thyroid and parathyroid glands extraction in H. javanicus (mals &females) declear (13) amino acid in males and (11) in female while in H. auritus (male &females) there were (12) amino acid in both male and females.

دراسة نوعية للبكتريا السالبة لصبغة كرام المعزولة من بيئة واخماج مستشفى عفك العام == Qualitative Study of Gram Negative Bacteria Isolated from Environment and Infections of Afak General Hospital

Author name: زينب عبد الكريم عليوي الفرحاني
Supervisor name: علي عبد الرحيم الناشي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: شملت الدراسة جمع 400 عينة سريرية وبيئية من مستشفى عفك العام في محافظة الديوانية للفترة من 1/11/2012 ولغاية 31 /3/2013 لغرض دراسة نوعية البكتريا السالبة لصبغة كرام والملوثة لبيئة واخماج المستشفى ومدى حساسية العزلات البكتيرية ومقاومتها للمضادات الحيوية والمطهرات شائعة الاستخدام .اظهرت هذه الدراسة ان 236 عينة وبنسبة 59% قد اعطت نتيجة موجبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 31 عينة من مصادر سريرية وبنسبة 56.37 % و206 عينة من مصادر بيئية وبنسبة 59.43% بينما كانت 164 عينة وبنسبة 41% سالبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 24 عينة من مصادر سريرية وبنسبة 43.63% و140 عينة وبنسبة 40.57 % من مصادر بيئية من مستشفى عفك العام .كانت العزلات البكتيرية السالبة لصبغة كرام المشخصة والبالغة 236 عزلة قد توزعت على اربعة اجناس وهي Pseudomonas شملت نوعين : P.aeruginosa 12.7% ، P. flourescens 8.5 % ، Klebsiella شملت K. pneumoniae 10.5% ، K.oxytoca 10.5% ، Serratia marcescens 3.4% ، Acenitobacterشملت A. baumanni 2.1% ، A.lwoffii 1.2% ، A.calcoceticus 0.8 %، Escherichia شملت E. coli 45.5% ، E.hermanni 4 % . كانت نسبة التلوث البكتيري في العينات السريرية الحروق والجروح قد بلغت 53.3 ، 60 % على التوالي ، بينما التلوث البكتيري في العينات البيئية المتمثلة بردهات دخول المرضى ، المطبخ ، صالات العمليات الجراحية ، ردهات الخدج وملابس العاملين في المستشفى قد شكلت النسب 66.6 ، 53.8 ، 60 ، 54.6 ، 40% على التوالي . كما اختبرت حساسية العزلات البكتيرية السالبة لصبغة كرام اتجاه 13 مضادا حيويا وقد اظهرت العزلات البكتيرية مقاومة وحساسية مختلفة تباينت حسب نوع العزلات البكتيرية وطبيعة المضاد الحيوي .كانت اعلى مقاومة اظهرتها بكتريا K.pneumoniae تليها K.oxytoca اتجاه مضاد Tetracycline اذ بلغت النسبة 96% و92% على التوالي . كما تميزت كل من بكتريا E.hermanni ، A.baumanni بمقاومتة عالية اتجاه ال Gentamycin اذ بلغت 80.3% ، 80% على التوالي اما P.aeruginosa فقاومت ال Tobramycin بنسبة عالية بلغت 90 %، بينما تميزت جميع البكتريا المعزولة في هذه الدراسة بحساسيتها اتجاه مضاد ال Imipenem . اما اختبار حساسية العزلات البكتيرية اتجاه المطهرات فقد ابدت مقاومة متفاوتة حسب نوع العزلات البكتيرية وتركيز وطبيعة المطهر وشملت المطهرات المستخدمة في التضاد الجرثومي كلا من الفورمالين والبوفيدين - ايودين والهبتين والديتول حيث كانت اعلى مقاومة اظهرتها E.coli اتجاه مطهر الفورمالين بتركيز 12.5% اذ بلغت 93.4% . درست بعض عوامل الضراوة المنتجة من الانواع البكتيرية ومنها انتاج المحفظة فوجد ان عزلات كل من بكتريا A. baumanni، P. aeruginosa ، K. pneumoniae وE.coli منتجة للمحفظة وبنسبة 40 ، 33.33 ، 100 ، 23.36 % على التوالي ، اما انزيم الهيمولايسين فوجد ان جميع عزلات A.baumanni غير منتجة لهذا الانزيم في حين جميع عزلات P. aeruginosa كانت منتجة لهذا الانزيم بنسبة 100% ، اما انزيم البروتيز فوجد اعلى نسبة لانتاجه في P.aeruginosa بنسبة 100 % وادنى نسبة في بكتريا E.coli وبنسبة 14 % ، كانت اعلى نسبة لانتاج انزيم الجلاتينيز من قبل كل من A.baumanni وP.aeruginosa وبنسبة 100 % وتميزت عزلات K.pneumoniae بعدم القدرة على انتاجه . | The study included the collection of 400 clinical specimens and environmental from Hospital Afak General in the province of Diwaniyah for the period from 1/11/2012 until 3/31/2013 for the purpose of study quality the gram negative bacteria and contaminated environment and hospital infections and the sensitivity of the bacterial isolates and their resistance to antibiotics and antiseptics commonly used . This study showed that 236 sample and 59% gave a positive result for the examination of bacterial for bacteria gram negative distributed among the 31 sample sources of clinical and increased by 56.37% and 206 samples from environmental sources and increased by 59.43% while the 164 sample and 41% negative for screening bacterial for bacteria gram negative distributed among the 24 samples from clinical sources, and by 43.63% and 140 sample and by 40.57% from environmental sources of Afak General Hospital. The bacteria gram negative isolates diagnosed, amounting to 236 isolation has distributed over four genera Pseudomonas They included two types : P.aeruginosa 12.7%, P. flourescens 8.5%, Klebsiella included K. pneumoniae 10.5%, K.oxytoca 10.5%, Serratia marcescens 3.4% , Acenitobacter included A. baumanni 2.1%, A.lwoffii 1.2%, A.calcoceticus 0.8%, Escherichia included E. coli 45.5%, E.hermanni 4%. The percentage of bacterial contamination in clinical samples of burns and wounds had reached 53.3,0.60% respectively, while bacterial contamination in environmental samples of lobbies entry of patients, the kitchen, Eat surgeries, lobbies premature and clothes of hospital personnel had formed ratios of 66.6, 53.8 0.60 , 54.6 0.40%, respectively. The sensitivity of the bacteria gram negative isolates tested for direction 13 have shown antibiotic - resistant bacterial isolates and different sensitivity varied by type of bacterial isolates and the nature of the antibiotic. The highest resistance exhibited by the bacteria K.pneumoniae followed K.oxytoca direction Anti Tetracycline as the percentage reached 96% and 92%, respectively. It also marked by both bacteria E.hermanni, A.baumanni resisting the trend of high Gentamycin reaching 80.3%, 80%, respectively, and P.aeruginosa resisting the Tobramycin high proportion reached 90%, while all characterized bacteria isolated in this study sensitivity counter direction the Imipenem . The test of the sensitivity of the bacterial isolates direction disinfectants have shown resistance varying according to the type of bacterial isolates and the concentration and nature of the cleanser included disinfectants used in contrast bacterial both Formalin and Povidin - Iodine and Hibtin and Dettol had the highest resistance exhibited by E.coli direction antiseptic Formalin concentration of 12.5% reaching 93.4% . I studied some of the virulence factors produced by bacterial species, including the production of the wallet and found that all isolates of bacteria A. baumanni, P. aeruginosa, K. pneumoniae and E.coli producing capsule and by 40, 33.33, 100, 23.36%, respectively, while the enzyme was found haemolysin that all isolates A.baumanni unproductive for this enzyme, while all P. aeruginosa isolates were producing this enzyme by 100%, while the enzyme protease and found a higher proportion of production in P.aeruginosa 100% and the lowest percentage in the bacteria E.coli and 14%, the highest percentage for the production of an enzyme gelatinase by both A.baumanni and P.aeruginosa and 100% K.pneumonae isolates were characterized by the inability to produce it

تجميع وتشخيص واستخدام الهائمات الحيوانية الدقيقة من نهر الحلة في تغذية يرقات اسماك الكارب الشائع (L., 1758) Cyprinus carpio

Author name: ازهار عبد الحميد عبد الحسن
Supervisor name: مؤيد جاسم ياس العماري | عدي جاسم عبد الرزاق
General topic: Biology
Specific topic: Environment
Degree: Master
University: University of Babylon - College Of Science - Department Of Biology
Language: English
University location: Babylon
First pages:
Abstract: The present study was carried out in AL - Hilla River near Al mahaweel district in the middle of Iraq during autumn 2016 to summer 2017. The study included a collection of high density from microzooplankton by use special device then identified and used it as live food for early feeding common carp larva (Cyprinus carpio). The environmental factors of Hilla river tested for four seasons and ranged between 6 - 44 ˚C of air temperature, 9.5 - 30 ˚C of water temperature, 7.03 - 8.58 of pH, 921 - 1090 µm/cm of conductivity, 654.33 - 772.00 mg/l of total dissolved solids and 550.00 - 636.33 of salinity, 5.43 - 13.03 mg/l of total suspended solids, 7.5 - 10.31 mg/L of dissolved oxygen, 1.96 - 6.30 mg/l of BOD5, 1.61 - 2.49 m/s of water velocity, 5.17 - 16.80 µg/L of chloropyll - a - . The taxonomic and ecological study is done to the microzooplankton specially rotifer. There are few studies of rotifer in Hilla river, The result showed that the density of rotifera was ranged between 23ind. /L in the summer season to 65.66 ind. /L in winter season, included 65 rotifer taxa (62 species +2genera +1 class) were recorded in this study, 10 taxa (9 species + 1 genus) were recorded for the first time in Iraq and 14 taxa (13 species + 1 genus) were recorded for first time in Hilla River compared with available local studies. Keratella tropica showed the highest density of the rotifera, Lecane genus showed the highest species number (10 species). Canonical correspondence analysis showed Physico - chemical parameters of water affected on the density of rotifer both positively and negatively. There was positive significant correlation between density rotifer with electrical conductivity, total dissolved solids, and salinity, while pH showed the lowest effect on the density of rotifer than other environmental factors. Different species of rotifer showed some variations of the correlation with environmental factor, such as the density of Bdelloidea class correlated positive significant correlation with water temperature, while Keratella cochlearis species negative significant correlation with water temperature. Special system (multi - filtration system) designed and made by researchers in our study for collection high density of microzooplankton with size sample range between 50 - less 200 µm, with short time, less effort and low cost. The multi - filtration system that consists of three stages : pumping stage, multi - filtration stage and sample collection stage. The density of the samples was measured seasonally by comparing the density of the microzooplankton in Hilla river and the density of the microzooplankton sample obtained by the multi - filtration system. The highest density of multi - filtration system rotifer was 1023033 ind./L and the highest density of multi - filtration system total microzooplankton was 1052000 ind/L and measured chlorophyll - a - of sample multi - filtration system that ranged between 23.54 - 1931.09 µg/L. The density of rotifer and total microzooplankton that collect by the multi - filtration system were concentration up to 20222.22, 17558.81 times than what is in the river. After collection of the high density of microzooplankton by using the multi - filtration system, this microzooplankton was used as live food for early feeding fish larvae. A special experiment was designed to feed common carp of fish larvae (Cyprinus carpio) using three types of food : first live diet collected by the multi - filtration system that microplankton most rotifer, second soybean meal and third mixed food (rotifer and soybean meal). Some growth indicators for fish larvae were studied; the results showed that the highest growth rate and best survival rate were larvae fed on live food, highest increased weight and increased length, relative growth rate, specific growth rate and lowest survival rate (33.53 mg, 3.33mm, 87.78, 30.11, and32.38 % respectively) for larvae fish feeding on rotifera, while lowest increased weight and increased length, relative growth rate, specific growth rate and lowest survival rate (27.00mg, 1.43mm, 85.23, 27.39, and 61.90 % respectively) for fish larvae feeding on soybean meal.

دراسة تصنيفية لبعض اجناس العويئلة الفراشية Papilionoideae في العراق == Comparative Taxonomic study of some genera Papilionoideae subfamily in Iraq

Author name: شيماء محي حسون
Supervisor name: نداء عدنان ابو سراج
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Babylon - College Of Science - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: اجريت هذه الدراسة في مختبرات كلية العلوم / جامعة بابل للفترة من شهر شباط 2016 الى شهر اب 2017 . وقد جمعت العينات من اماكن مختلفة من شمال العراق ووسطه . وقد تناول البحث الحالي دراسة تصنيفية مقارنة شملت بعض الصفات المظهرية العامة والدقيقة للبذور وكذلك دراسة صفات حبات اللقاح , ودراسة الصفات التشريحية لبعض الاجزاء النباتية , فضلا عن الدراسة الجزيئية . وشملت الدراسة تسعة عشر نوعا تعود تسعة اجناس مختارة من عشائر مختلفة من العويئلة الفراشية في العراق , وتضمنت نوعان للجنسAstragalus L. هما A.kahiricus DC. وA.rhodosemius Boiss.and Hausskn ونوع واحد لكل من الاجناس L. Coluteaالنوع Col.cilicica Boiss. ; Coronilla L. النوع Cor. scorpioides (L.)W.D.Koch. ; Glycyrrhiza L. الضرب G.glabra var. glandulifera (Waldst. et Kit) Regel et Herd. ; Hippocrepis L. ; النويع Hi.unisiliquosa L. subsp.bisiliqua (Forssk.)Bornm. ; Hymenocarpos Savi. النوع Hy.circinnatus (L.) Savi. ; Pisum L. النويع P.sativum subsp. elatius (M.Bieb.) Aschers. بالتحديد الضرب var.pumilio Meikle ; والجنس Scorpiurus L. الضرب S.muricatus var.subvilosus (L.) Lam. , فضلا عن تسعة انواع للجنس Onobrychis Mill. هي O.acaulis Bornm. , O.caput - galli (L.) Lam. , O.crista - galli (L.) Lam. , O.carduchorum Townsend , O. megatophros Boiss. , O.haussknechtii Boiss. , O. galegifolia Boiss. , O.ptolemaica subsp. macroptera Townsend. , O.ptolemaica subsp. ptolemaica (Del.) DC. , O.schahuensis Bornm.. اقتصرت الدراسة المظهرية على الصفات المظهرية العامة والدقيقة للبذرة باستعمال المجهر الضوئي Light microscope والمجهر الالكتروني الماسح Scanning electron microscope لسطح غلاف البذرة , وقد دلت النتائج على اهمية صفات البذرة من الناحية التصنيفية , اذ تم تحديد ابرز الصفات والاكثر اهمية في عزل العشائر والاجناس زيادة على عزل الانواع , لاسيما شكل البذرة ولونها وعدد البذور في البقلة , علاوة على الزخرفة السطحية لغلاف البذرة والتي لها الدور البارز في الفصل بين افراد العشيرة Coronilleae , كما كان لها الدور في فصل كل من العشائر المدروسة عن بعضها . اما صفات حبات اللقاح فقد كانت من الصفات المظهرية ذات القيمة التصنيفية العالية لعزل العشائر والاجناس , فقد دلت النتائج وجود اثنان من الطرز المختلفة هما : Colpate وColporate اذ امكن تمييز حبات لقاح الجنس Onobrychis بكونها ذات حبات لقاح من النوع Colpate , كما فصلت حبات لقاح النوع Hymenocarpos circinnatus عن باقي الاجناس بالاستناد الى عدد فتحات الانبات فقد تميز بكون حبات لقاحه سداسية وسباعية فتحات الانبات , كما كان لشكل وحجم الحبة وشكل الاخدود فضلا عن صفات الزخرفة السطحية لجدار الحبة المدروسة تحت المجهر الالكتروني الدور التصنيفي الجيد في فصل الانواع قيد الدراسة. اما جانب الدراسة التشريحية فقد شملت الدراسة صفات البشرة للوريقات والكاس الزهرية والتويج (ورقة العلم) والسيقان , فضلا عن دراسة المقاطع المستعرضة للسيقان ونصول الوريقات والسويقات وحاملات النورات والثمرة (البقلة) . كما شملت الدراسة طبيعة الكساء السطحي وانواع البلورات المنتشرة وانماط اﻠﺗﻌﱠرق Venation patterns. وقد دلت النتائج الى اهمية كل الجوانب التشريحية المدروسة في الفصل بين الاجناس المدروسة وكذلك بين الانواع. وتضمنت الدراسة ايضا التصنيف الجزيئي باستعمال تقانة البلمرة المتسلسل Polymerase chain reaction (PCR) من خلال معرفة تتابع Sequencing المنطقة النووية ITS اذ كان للدراسة الجزيئية اهمية تصنيفية لمعرفة علاقات التقارب والتباعد بين انواع الاجناس المدروسة , وتم التعرف على النوع A.rhodosemius كتسجيل جديد للجنس ضمن الانواع المنتشرة في العراق . | This study was conducted in the laboratories of the Faculty of Science / University of Babylon for the period from February 2016 to the month of August 2017. Samples were collected from various parts of northern Iraq as well as in the central regions .The present investigation deals with a comparative taxononmic study , included some gross and micromorphological characters , as well as the pollen grains characteristics and anatomical characteristics of some plant parts , as well as the molecular study . The study included nineteen species belonging to nine selected genera from different tribes of papilionoideae subfamily in Iraq , and included two species of genus Astragalus L. ( A.kahiricus DC. and A.rhodosemius Boiss.and Hausskn ) , and one species of each genera Colutea L. (Col.cilicica Boiss.) , Coronilla L. (C.scorpioides (L.) W.D.F.Koch) , Glycyrrhiza L. (G.glabra var.glandulifera (Waldst. et Kit) Regel et Herd) , Hippocrepis L. ( Hi.unisiliquosa L.subsp.bisiliqua (Forssk.)Bornm. ) , Hymenocarpos Savi. (Hy.circinnatus (L.) Savi. ) , Pisum L. (P.sativum subsp. elatius (M.Bieb.) Aschers. var.pumilio Meikle. ) and Scorpiurus L. ( S.muricatus var.subvilosus (L.) Lam. ) , in addition to nine species of genus Onobrychis , O.acaulis Bornm. ; O.caput - galli (L.) Lam. ; O.crista - galli (L.) Lam. ; O.carduchorum Townsend. ; O. megatophros Boiss. ; O.haussknechtii Boiss. ; O. galegifolia Boiss. ; O.ptolemaica subsp. macroptera Townsend. ; O.ptolemaica subsp. ptolemaica (Del.) DC. ; O.schahuensis Bornm. The morphological study was limited to gross and micromorphological characters of seed using light microscopy and a scanning electron microscopy for the surface of seed coat . the result indicated the taxonomic importance of the seed characteristics , the most important valuable in isolating the tribes and genera , also species. Especially the seed shap , colour and the number of seeds in the pod . In addition to the surface ornamentation of seed coat , Which had a important role in the separation of members of the tribe Coronilleae, and had a role in the separation of each of the studied tribes. The characteristics of pollen grains have good taxonomic value in isolation of tribes and species . the results indicated that there are two different types of pollen grains , colpate and colporate , the polle grains of Onobrychis can be distinguished from other genera by lacking germinating aperture ,pollen grains of Hymenocarpos circinnatus was separated from the other of the species based on the number of germination pores , The shape and size of the grain and the shape of the colpus as well as the characteristics of the surface ornamentation of the grain wall studied under the electronic microscope, the role of good classification in the class of species under study. pollen grains of other genera were separated based on the characteristics of germination pore , the shape , size of the grain and the shape of , in addition to the characteristics of ornamentation of studied grain wall under the electron microscope . As the anatomical study , included the characteristics of the epidermis of leaves , the calyx , corolla and stems , as well as the study of transverse sections of stems , leaflets petioles , peduncles and the pod . also the nature of indumentum , types of crystals , and venation patterens . the results showed the importance of all anatomical aspects studied in separation between the studied genera as well as between species. The study also included molecular systematic using polymerase chain reactions (PCR) through sequencing of nuclear (ITS) region . it was clear that the molecular study has taxonomical significance to know the phylogeny and relationships of convergence and divergence between species . A.rhodosemius was identified as a new species registry in Iraq.A.rhodosemius is recognized as new species for Iraq.

دراسة جزيئية للجينات البيتا - لاكتاميز في بكتريا Acinetobacter baumannii المقاومة للمضادات الكاربابينيم == Molecular Study of ? - lactamase Genes in Carbapenem Resistant Acienetobacter baumannii (CRAB)

Author name: ربيع عبد الاله مجيد عبد علي الهنداوي
Supervisor name: ايمان محمد جار الله
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Babylon - College Of Science - Department Of Biology
Language: English
University location: Babylon
First pages:
Abstract: During the period between April 2016 and January 2017, a total of 1100 clinical specimens (burns, wounds, throat, urine, blood) were collected from two hospitals in Babylon province Al - Hillah Teaching Hospital and Medical City of Mirjan Hospital. All specimens were cultured and 17 Acinetobacter baumannii isolates were obtained from clinical specimens in a percentage of (1.5%) distributed in burn 11 (2.8%), wound 1 (0.6%), throat 1 (2%), urine 3 (0.7%) and blood 1 (0.9%).This study was conducted to determine the presence of carbapenem resistance Acinetobacter baumannii (CRAB) isolates and the prevalence of β - lactamase genes responsible for carbapenem - resistance among clinical isolates of A. baumannii collected from two hospitals in Hillah City. Isolates were identified according to VITEK2 system. Antibiotics susceptibility was assayed by using disks diffusion method according to CLSI recommendations and it’s found 8 isolates was carbapenem resistant.The eight CRAB isolates were tested for biofilm formation by using the tube adherence test and the results showed that 5 of 8 tested isolates (62.5%) were strong for biofilm formation. Two genes related to biofilm formation was investigated (bap & blaPER - 1 genes). Six isolates were positive for the bap gene (75 %) and no isolates had positive for blaPER - 1 gene. The eight CRAB isolates were tested also for the presence of two virulence factor genes include espA (K1 capsular polysaccharide) and ompA (outer membrane protein A), this detection was performed by the conventional PCR technique. The positive result for espA and ompA genes was (62.5%) and (75%) respectively.IIPhenotypic detection of carbapenemase production was performed using the imipenem - EDTA disk and modified Hodge's test (MHT). Then isolates were subjected to monoplex PCR targeting blaCTX, blaTEM, BlaSHV, blaIMP, blaVIM, blaSPM, NDM - 1, NDM - 2, AmpC, OXA - 23, OXA - 24, OXA - 48, OXA - 51, OXA - 58 and OXA - 235 genes, all CRAB isolate gave positive result with MHT and (50%) of these isolates were positive to the imipenem - EDTA disk. PCR experiments showed ; all CRAB isolates were harbored blaOXA - 51 gene, six (75%) isolates were harbored blaCTX gene, four (100%) isolates were harbored blaTEM gene, four (50%) isolates were harbored blaVIM gene, four (50%) isolates were harbored blaSPM gene, seven (87.5%) isolates were harbored NDM - 2 gene, seven (87.5%) isolates were harbored ampC gene, six (75%) isolates were harbored OXA - 23 gene, one (12.5%) isolates were harbored OXA - 24 gene, two (25%) isolates were harbored OXA - 48 gene and one (12.5%) isolates were harbored OXA - 235 gene, while none of these isolates harbored BlaSHV, blaIMP, NDM - 1, NDM - 2 genes. All isolates of A. baumannii appear as MDR, while two isolates appear to be as XDR.These results revealed that CRAB isolates constitute 47% of all A. baumannii that had been isolated from Al - Hillah Teaching Hospital and Medical City of Mirjan Hospital in Babylon province and some of carbapenemase were detected in both phenotypic and genotypic methods (PCR). This underlies the importance of their accurate identifications and reporting to prevent the emergence of complete resistance to the most potent drugs against A. baumannii in Babylon province.
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