دراسة تشخيصية ووبائية لطفيلي المقوسة الكوندية لطلبة جامعة ذي قار باستعمال تقنيتي الاليزا Toxoplasma gondii وتفاعل سلسلة البلمرة ذو الوقت الحقيقي == Diagnostic and Epidemiological Study of Toxoplasma gondii for Students of Thi - Qar University by ELISA and Real - Time PCR Techniques
Author name:
رباب علي شناوه الموسوي
Supervisor name:
بسعاد عقرب العبودي | منال بادي التميمي
General topic:
Biology
Specific topic:
Life Science
Degree:
Master
University:
University of Thi-Qar - College Of Science
Language:
English
University location:
Dhi Qar
First pages:
24T3258 - p.pdf
Abstract:
Toxoplasma gondii is a unique intracellular parasite, which infects a large proportion of the world, population, but clinically uncommonly causes significant disease. The present study was performed for the first time in Thi - Qar province to estimate the prevalence of toxoplasmosis among university students. Blood samples were collected from 319 (111 males and 208 females) apparently healthy students, they have ages between (18 - 42) years attended from different colleges of Thi - Qar university (students of morning and evening study), during the period from October 2013 to April 2014.Enzyme linked Immunosorbant Assay (ELISA), was used to evaluate the presence of anti - Toxoplasma IgM and IgG antibodies and detection of B1 gene of T.gondii DNA by Real - Time Polymerase Chain Reaction (RT - PCR).The results indicated that 21.94% of students were exposed to positive for anti - Toxoplasma antibodies, 5.33% of them had IgM, 11.91% had IgG, and 4.70% had both IgM and IgG. Statistically, they were significant difference between them.There were a significant difference between males and females in total rate but not significant of antibodies pattern in them, which recorded 7.52% of males and 14.42% of females were positive for anti - Toxoplasma antibodies.The study demonstrated that no significant difference in infection percentages between colleges where students studied, although the highest seropositivity students was 47.83% in education sports college whereas it did not score any infection with toxoplasmosis in College of Education for Pure Science .The current study also showed that the highest positive percentage with T.gondii of male students was 28.57% in College of Engineering while the highest percentage with T.gondii of female students was 30.43% in College of sports education.The present study revealed that the acute infection of toxoplasmosis which presented IgM and both IgM and IgG was high at small age youngstudents (18 - 22) years while the chronic infection which presented IgG was high at old students (38 - 42) years.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. Although more students in the positive sample group eating undercooked meat but not significant difference between those 36.13% and other students who did not have this habit 13.5% has been discovered.The results showed the highest positive percentage samples at middle socioeconomic level was 24.10% while the lowest percentage at good socioeconomic level was 19.60% .The results showed that the highest positive percentage samples 31.0% at Refaee district whereas the lowest percentage was 19.78% as noticed at Naserya district.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with presence of clinical symptoms (fever, headache, malaise, influenza) that may have a relationship with toxoplasmosis of infected students when compared with not presence clinical symptoms of those (p<0.05) they recorded 18.57% and 81.43% respectively.The present study revealed an association between blood group system and Toxoplasma infection with highest prevalence among samples of blood group A+ 30.65% and lowest prevalence in samples of blood group B+ 17.44% .The current study evolutes the rate of some blood parameters, in infected and non infected students, there was no significant difference observed in mean hemoglobin level (Hb), total count of leukocytes (WBCs), neutrophilis, lymphocytes, monocytes and basophilis numbers in both student . While significant differences were observed in mean of eosinophils number of infected students was more than number of non infected students.Moreover, mean of lymphocytes number recorded significant differences between infected and non infected females only.Besides the serological diagnosis of T.gondii, Real - Time PCR (RT - PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the blood of students. Out of 319 students only 6.26% showed positive for toxoplasmosis among those 2.50% were males and 3.76% were females. The positive result in RT - PCR analysis were distributed on the patterns of the anti - Toxoplasma antibodies by ELISA test , Any positive blood samples with IgM, IgG and both IgM and IgG respectively have not been found whereas 20 positive cases of no anti - Toxoplasma antibodies.Real - Time PCR test in student's blood has advantages in detection of recent or active toxoplasmosis
