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التاثيرات النسجية والدمية وبعض النواحي الوظيفية لعقار البيروكسيكام في ذكور الفئران البيض Mus musculus Histological And Hematological Effects And Some Physiological Aspects of Piroxicam In Male Albino Mice Mus Musculus

اسم المؤلف: بشرى عمر احمد معروف
اسم المشرف: انتظار محمد مناتي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The current study aimed to detect the effect of Piroxicam in making behavioral, hematological and physiological changes in the kidneys of adult male albino mice Mus musculus, The present study also included measuring the diameters of glomeruli, proximal and distal convoluted tubules as well as the histopathological study.A total number of (128) adult male albino mice were utilized in this study, they were randomly distributed into four main groups, the first three groups were orally treated with concentration (50, 100, 150) mg/kg respectively, the forth group considered as control group were orally treated with (0.1) ml of distilled water for eight weeks, the samples were collected every two weeks for the histological study, blood samples were withdrawn for the hematological and physiological studies.The current study showed that there were behavioral changes observed on the experimental animals, represented by reluctance to food intake, lethargy, tremor, introversion, fast breathing, change in eye’s color and blackened feces, and the intensity of these symptoms increases with the increment of concentration and administration period.The statistical study results revealed that there was significant decrease at (P<0.05) in the mean numbers of red blood corpuscles, hemoglobin concentration and the percentage of packed cells volume in orally treated groups with (50, 100, 150) mg/kg for (6, 8) weeks of administration in comparison with control group, as for the white blood cells the statistical results for this study showed significant increase (P<0.05) in their mean number in treated groups with concentration (100, 150) mg/kg for (6, 8) weeks of administration compared to the control, whereas the groups treated with concentration of (50) mg/kg showed no significant changes along the administration period.Also all the concentrations showed significant differences (P<0.05) in the mean concentration of urea, creatinine and potassium ion in the blood serum of orally treated mice at the last weeks of administration in comparison with the control group.The statistical results of the present study also revealed that there were significant differences (P<0.05) in the mean diameters of renal glomeruli of the treated groups with concentration (50, 100, 150) mg/kg for eight weeks while there were non - significant differences in other periods, also there was non - significant decrease in the mean diameters of proximal convoluted tubules in the treated groups with concentration of (50, 100, 150) mg/kg in all periods except the period four weeks it showed a significant decrease (P<0.05) in the mean diameters of proximal convoluted tubules of the groups treated with the concentration (100, 150) mg/kg in comparison with control group. The statistical results also clarified that there were significant differences (P<0.05) in the mean diameters of distal convoluted tubules of the treated groups with concentration (100, 150) mg/kg for six and eight weeks of administration, while the group treated with concentration (50) mg/kg had no significant differences over the whole administration period.The study also recorded the presence of histopathological symptoms in the kidneys of treated mice with the dose (50)mg/kg represented by hemorrhage, vascular congestion, calcium casts formation inside the lumens of renal tubules, while the groups treated with (100)mg/kg of the drug showed a fibrin deposition inside renal tubular lumens, infiltration of inflammatory cells, cytoplasmic vacuolation in the epithelial cells of the renal tubules, and sloughing epithelium of renal tubules, while in the medullary region, there were epithelial cells atrophy in the renal tubules. The histological examination of mice kidneys treated with (150) mg/kg showed the appearance of hyalinization, shrinkage and complete loss of glomeruli, Pyknosis of nucleus and fibrosis around the blood vessels as well as the presence of oedema which occupied a large area of the kidney tissue

تقييم نوعية المياة الصناعية ووحدات المعالجة لمعمل النسيج القطني في الكاظمية Evaluation of The Industrial Wstewater And Treatment In Al - Kadhimia Cotton Weaving Industry

اسم المؤلف: اسيل حسين محمد السعداوي
اسم المشرف: سهير ازهر الكاظمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study includes two axis, the first one determined the physical and chemical characteristics for several concentrations of industrial wastewater of cotton textile factory, that located in Al - Kadhmiya, which are disposable to the main assembly in the treatment unit (first unit) of the factory ; Also the final water quality was evaluated after the treatment in the fourth unite and compared with normal environmental averages (or compared with acceptable limits for discharged.The second axis of this study assessed the efficiency of the physical and chemical treatments in the third unit, and also the biological treatments in the fourth unit through detecting the removal percentage for each stage of the treatment to evaluated the treatment unit efficiency of this factory. Samples were collected from four treatment units for six month, single sample was collected per two weeks.The (local, field) tests were assessed for the collected samples including (Temperature, PH, DO and EC, while the laboratory tests included TSS, TDS, BOD, COD, oil and grease, So4 - 2 and heavy metals such as Pb, Zn and Cr).The results showed that the temperature values were with (normal range) acceptable limits (22.3C?) in the first unit and (19.8C?) in the fourth unit; PH values also were within normal limits in the almost treatment units (neutral), however, the electrical conductivity average was 3123?c/cm in the first unit, and 1101 ?c/cm in the fourth unit ; Moreover, the average values of COD were 580 mg/L in the first unit and 163 mg/L in the fourth unit. The removal percentage was 41% in the primary sedimentation in thesecond unit while it was 36% in the third unit for biological treatment.The BOD values were 487 and 302 mg /L in the first and fourth unit respectively ; the removal rates were 27% and 29% for primary sedimentation stage and biological treatment respectively and these rates are acceptable for normal limits.In general, the final treatment concentration was 302 mg/L in the fourth unit which is normal environmental rang, and also BOD concentration was not exceeded the environmental limits in the final stage of the treatment while the total percentage dissolved salts were 37% and 22% for the third unit which are normal in comparison to the Iraqi limits after the treatment for the fourth unit (703mg/L). Furthermore, the results of suspended solids found that the removal percentage was 76% for the second unit and 43%for the third unit while the total concentration of the last treatment was 61mg/L which is with normal limits, also the concentration of lipids of oils and grease was 0.8 mg/L for the fourth unit, while the removal percentage was efficient for the primary sedimentation and biological treatment. The heavy metals concentraitions were now with water and the main assembly tank and this was within normal environmental limits.

التحري عن طفرة JAK2V617F والمستويات المصلية لانزيمي الفوسفتيز القلوي واللاكتيت ديهايدروجنيز في مرضى ابيضاض الدم النخاعي المزمن Detection of Jak2V617F Mutation And Serum Levels of Alkaline Phosphatase And Lactate Dehydrogenase In Chronic Myelogenous Leukemia

اسم المؤلف: استبرق اكرم بيرام الحسيني
اسم المشرف: عصام فاضل علوان الجمیلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد مرض ابيضاض الدم النخاعي المزمن (CML) من الاورام النخاعية التكاثرية، وينشا نتيجة تكون جين Bcr - Abl (الذي يسمى كروموسوم فيلادلفيا) داخل الخلايا الجذعية المكونة للدم. يؤدي هذا الشذوذ الجيني الى تفعيل متواصل لانزيم التايروسين كاينيز وبالتالي نمو وتكاثر غ | Chronic myelogenous leukemia (CML) is a myeloproliferative neoplasm arises from Bcr - Abl gene translocation (called Ph chromosome) in hematopoietic stem cells (HSCs). This genetic abnormality results in constitutive activation of tyrosine kinase and subsequent uncontrol growth and multiplication of granulocytes. The cornerstone in treatment of CML are tyrosine kinase inhibitors, of which imatinib is the most effectively used. JAK2V617F mutation is an acquired single nucleotide polymorphism (SNP) occurs in JAK2 gene and is associated with many hematological malignancy other than CML. It was thought that the two genetic abnormalities (Bcr - Abl and JAK2V617F) occur mutually; however, growing body of evidences suggested the reverse. This study aimed to investigate the prevalence of JAK2V617F mutation associated with serum levels of alkaline phophatase (ALP) and lactate dehydrogenase (LDH) in Ph+ CML Iraqi patients treated with imatinib. A total of 43 Ph+ CML patients (25 males and 18 females, age range 16 - 80 years) who attend Iraqi National Center of Hematology for Research and Treatment/Baghdad were enrolled in this study. Each patient has been received at least six month therapy with imatinib. A consent form involving age, gender, height, weight, smoking status, residency and first family relative history of leukemia was obtained from each patient. Besides, blood samples were collected, from which the granulocytes were separated and then DNA was extracted using a ready kit. Two assays were used for detection of JAK2V617F mutation; real time polymerase chain reaction (qPCR) using specific primers and probe, and allele specific PCR (AS - PCR) using specific primers. Total white blood corpuscles (WBC) as well as serum levels of ALP and LDH were measured. qPCR assay revealed 5 patients out of 43 (11.62%) were heterozygous for the muatant allele of JAK2V617F mutation (genotype GT). The concentration of this allele ranged from 0.01% to 0.12%. None of blood sample gave positive result for AS - PCR assay. From the all risk factors, only gender had significant association with the incidence of JAK2V617F mutation. Average total WBC count, and serum levels of ALP and LDH were higher in JAK2V617F - positive patients (9042±1512.55, 146.05±8.028 IU/L and 204±10.85 IU/L respectively) than that of JAK2V617F - negative patients (6039±1772.239, 64.45±40.15 IU/L and 178.33±13.693 IU/L respectively) with significant differences. These results indicate that JAK2V617F mutation can occur simultaneously with Ph chromosome in CML patients, and qPCR is a highly sensitive method for the detection of this mutation. Furthermore, serum activity of ALP can be used as an indicator for the presence of JAK2V617F mutation in CML patients.

الدور الوقائي لبكتريا البفديس ضد خمج الفئران ببكتريا الاشيريكيا القولونية المنتجة لذيفان الشيكا The Protective Role of Probiotic Bifidobacterium Against Mice Infection With Shiga Toxin Producing E.Coli O157 : H7

اسم المؤلف: سمر مصطفى محمد
اسم المشرف: شادان عباس الوانداوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Six Bifidobacterum isolates were isolated from fully breast - fed healthy infant faeces on reduced de Man Rogosa and sharp medium (MRS - C). Isolates identified on the basis of, colonial and microscopical properties, biochemical tests, and fructose - 6 - phosphate phosphoketolase enzyme (F6PPK) activity assay in cellular extracts. Carbohydrates fermentation profile used for identification of isolates to species level. All bacterial isolates diagnosed as Bifidobacterium genus where in this study B. adolescntis was the predominant species (50%), (B4, B5 and B6), followed by B. breve (B3), B. longum (B1) and B. dentium (B2) each one represent 16.67%.Bifidobacterium isolates were screened for their antagonistic effects against test organism, clinical isolate of shiga toxin producing E.coli O157 : H7 (STEC), using agar - well diffusion method. The isolates B3 and B6 showed clear inhibitory actions, 22 mm and 15 mm diameter of inhibitions zones, respectively. The rest of the tested isolates did not pronounce any inhibitory activity.B. breve in vivo antagonistic behavior and the possible protective effects against STEC was evaluated, using streptomycin treated murine model. Murine intestines was stably colonized orally with B. breve for 14 days, in conjunction mice were challenged orally with STEC, 103 CFU / mouse / day on day 8 of experiment. Bacterteriological analysis of mice faeces at time intervals, was indicated high levels of bacterial colonization were achieved in intestine by B. breve and STEC.Colonization of mice intestine by B. Breve did not inhibited STEC cells from proliferation during infection phase. Hence, the excretion level of STEC in faeces reached to 2.4 x 10 6 CFU/ g of faeces.STEC infected mice showed no severe clinical signs, characterized by hairloss, lethargy, paralysis of fore limbs, and shed of loose faeces. In the B. breve - colonized group, the mentioned clinical signs were almost completely inhibited, except the lethargic of some animals.Immunological studies showed an increase in the levels of sIgA by 2.7 - fold from that of blood IgA in B. breve - colonized mice while, reversed values were recorded in mice infected with STEC, blood IgA level was 1.95 - fold higher than that of sIgA.Histological changes in spleen, liver, kidney, and intestine tissues of mice were studied. The histological sections clarified the protective roles of B.breve, where no effective histological disorders were appeared in B.breve and STEC - colonized mice. In the STEC - infected mice, the pathological abnormalities within the kidney was the predominant, diagnozed as ulcers in the lining membranes, glomerular and tubular epithelium necrosis, without evidence of glomerular thrombi, mild damages was appeared in liver and spleen, and characteristic attaching and effacing (A / E) lesions appeared in the large intestine sections

الاصابة ببكتريا Helicobacter pylori وعلاقتها بمرض تصلب الشرايين القلبية Correlation Between Helicobacter Pylori Infection And Atherosclerotic Heart Disease

اسم المؤلف: رواء سعدي سلمان
اسم المشرف: مي خليل اسماعيل
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: مرض قصور الشرايين التاجية هو النتيجة النهائية لتراكم اللويحات العصيدية ضمن جدران الشرايين التاجية مسببة نقص الاوكسجين وبالتالي حدوث مرض القلب الاقفاري. ويعتبر من الامراض الشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم. اضافة الى عوامل الخطورة التقلي | Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in shortage of oxygen supply and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of worldwide morbidity and mortality. In addition to traditional risk factors for cardiovascular disease, nowadays, accumulating evidence indicates that a variety of infectious agents may contribute to pathogenesis of CAD. The present study evaluated the anti - Helicobacter pylori IgG and IgA and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) and vacuolating associated cytotoxin (Vac A) as a risk factors for CAD.Eighty patients were divided into 2 groups : first group was 70 patients with CAD, the other group contained 10 patients with another coronary artery disease; they were admitted to Ibn Al - Bitar Specialist Center for Cardiac Surgery in Baghdad between October 2013 and January 2014. Ten individuals used as a healthy control group. All blood samples were tested biochemically such as glucose test, urea test, creatinine test and lipid profile test(cholesterol, triglyceride, LDL and HDL) and all of them showed normal results. The present study revealed that males were affected more than females (3 : 1); with no relation between their ages and bacterial infection.Serum IgG and IgA was Estimated by indirect immunofluorescent (IIF) whereas Cag A, Vac A and high sensitive C - reactive protein (hs - CRP) measured by enzyme linked immunosorbent assay (ELISA) and compared to control group results.The incidence of the anti - H.pylori IgG was highly significantly prevalent in CAD patients 78.57% (55/70), than in control group 0% (0/10), also a high significant difference in another CAD patient group 100%(10/10) as compared to control group 0% (0/10) (P? 0.01).Similarly anti - H. pylori IgA in CAD patients and another CAD patient groups showed highly significant increase when compared to control group 37.14% (26/70), 30% (3/10) and 0% (0/10) respectively (P? 0.01).The incidence of the CagA - positivity was significantly prevalent in mean value (2.74±0.19) in patients with CAD and another CAD patient group(2.72±0.31) than in control group (1.64± 0.16) (P? 0.05). Same result was observed with Vac A antigen; mean value of patient group showed significant increase (1.299 ± 0.04) when compared to controls group (1.41±0.13) (P? 0.05). Also significant increase found between another CAD patient group(1.79±0.17) and control group (1.41±0.13) (P? 0.05). Furthermore; the present study revealed significant differences of concentration levels between hs - CRP in CAD patient group (4.95 ± 0.38 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml), as well as a significant differences found between another CAD patient group (3.96 ± 0.96 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml) (P? 0.05).

التاثيرات السمية لجسيمات الفضة النانوية على بعض الاعضاء لاناث طائر السمان Coturnix coturnix : دراسة نسجية وكيموحيوية Toxic Effects of Nanosilver Particles On Some Organs of Female Quail (Coturnix Coturnix); Histological And Biochemical Study

اسم المؤلف: عبير ساجد عبد علي
اسم المشرف: غزوة درويش النقيب
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة التعرف على تاثير جسيمات الفضة النانوية في نسجية كل من الكبد والطحال والكلى فضلا عن تاثيرها في وظائف الكبد والكلى من خلال قياس بعض انزيمات الكبد المتضمنة (AST & ALT) وقياس مستوى اليوريا والكرياتنين (Urea & (Creatinine, فضلا عن تاثيرها في | This study was included to identify the effect of silver nanoparticles on histology of the liver, spleen and kidney, as well as it's effect on functions of the liver and kidneys through the measurementof some liver enzymes included (AST and ALT) and measurement of urea and creatinine levels, as well as the effect of silver nanoparticles in the weight of studied samples, numbers and sizes of the eggs were produced through the exposure periods.(45) quail (females) were collected from agricultured research center in Abu - Ghraib, divided into (4) groups including : T1 (12 quails were exposed to 4ppm), T2 (12 quails were exposed to 8ppm) and T3 (12 quails were exposed to 12 ppm) of nanosilver particles solution for (60) days.As well as control group T4(9 birds were exposed to distilled water).After the end of the exposure period samples were sacrificed by discapitation of the neck, and blood was collected for the purpose of serum separation which required for biochemical study, samples were dissected and fixated with Bouin's fluid, histological slides were prepared and stained with Eosin - Hematoxylin.Biochemical study were showed significant decrease in the level of liver's enzymes (AST & ALT), urea and creatinine. This decrease was elevated when the concentration was increased, as well as significant decrease in each of sizes and numbers of the eggs were produced through exposure periods and non - significant decrease in the body weight.Exposed groups with silver nanoparticles showed histopathological alterations for each of liver, spleen and kidney, in liver included congestion of blood vessels, infiltration of inflammatory cells, exudate, appearing of concilman bodies, fatty degeneration in most of hepatocytes which progressed to fatty changes in most of samples were exposed with 12ppm, Hyperplasia for each of blood vessel walls and capsule, atrophy of hepatocytes, and appearing of giant cells, while kidney sufferd from hydropic degeneration in most of renal tubules hemorrhage for each of cortex, medulla and glomerulus, hyaline degeneration of tubules, amyloid precipitation for each of glomerulus and blood vessels, sloughing of epithelial cells of renal tubules from basement membrane, hypertrophy of renal tubules with hyperplasia for cells of tubules and atrophy of renal tubules.Spleen tissue showed decreased in sheets of white pulp and lymphocyte numbers red pulp cells. Severity of these effects varied depending on individual differences between samples and concentration of silver nanoparticles, which increase in samples were exposed with highest concentration.

عزل وتشخيص بكتريا Streptococcus spp المسببة لالتهاب اللوزتين من الاطفال وتاثير بعض عزلات بكتريا حامض اللبنيك عليها Isolation And Identification of Streptococcus Spp Caused Tonsillitis From Children And The Effect of Some Lactic Acid Bacteria (Lab) Strains On It

اسم المؤلف: رند ثائر عبد اللطيف
اسم المشرف: ندى صباح رزوقي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: بحثت الدراسة جمع 100 مسحة من اطفال مصابين بالتهاب اللوزتين الحاد والمزمن ممن راجعوا استشارية الانف والاذن والحنجرة في مستشفى اليرموك التعليمي للمدة مابين كانون الاول للعام 2013 ولغاية اذار للعام 2014. وكانت نتيجة الزرع المختبري موجبة في 67 مسحة منها وبالا | In this study 100 swabs were collected from infected children with acute and chronic tonsillitis who attended at Al - Yarmook Teaching Hospital (Eear Nose Tonsils consultation clinic) from December 2013 until March 2014. The result of laboratory culture were positive in 67 samples. Depending on their cultural, morphological and biochemical characterization of bacterial isolates : (37.31%) of them were identified as Streptococcus pyogenes bacteria and the diagnosis is confirmed by the use of Remel Rapid STR System. Whereas (34.33%) belong to S.parasanguinis, (11.94%) S.mitis, (11.94%) S.oralis and (4.48%) S.thoraltensis were identification of them are confirmed by using Vitek - 2 System also. As well as Sensitivity test for S.pyogenes cultures for some of the antibiotics done and the results revealed that all cultures were sensitive to tetracycline and chloramphenicol drugs 100%, while it was different in their sensitivity for other drugs. According to age (2 - 13) years revealed the highest percentage of infection for both male and female, however the percentage of acute and chronic tonsillitis was higher in males than females. In this study, during investigation of the antagonist action of lactic acid bacterial isolates against these pathogenic bacteria, results revealed that the method of L.acidophilus discs gave a high inhibition zones with a diameter of 11.3 mm, while L.fermentum discs yielded a high inhibition zones with a diameter of 7 mm 24 hrs following incubation in comparison with well diffusion method by using suspension of L.acidophilus produced a high inhibition zones with a diameter 7.25 mm 48 hrs after incubation. L.fermentum suspension produced a high inhibition zones with 10 mm diameter 24 hrs following incubation. Also the study included measurement of the inhibition activity for bacteriocins produced by L.acidophilus bacteria against the pathogenic bacteria S.pyogenes on nutrient agar by well diffusion method in which results revealed stability of the bacteriocins effects under different PH (4, 5, 6) with percent (100, 60, 50)% respectively for 24 hrs at higher stability and the highest stability of bacteriocins during PH was 4 with percent 100%, while it lost a lot of its activity with acidic PH less than 2 and basal pH more than 8. During the study the effect of Nacl, Kcl and MgSo4 with different concentrations (1 - 5%) with constant inhibitory effect for bacteriocins produced by L.acidophilus against pathogenic S.pyogenes, The result revealed that Nacl had little effect in inhibition zone with 1&2% concentrations. The salt MgSo4 and Kcl showed reduction in the inhibitory activity with 1, 2, 3% concentration, however the higher concentration of salt caused great reduction as 5% concentration led to loss of inhibitory activity for bacteriocins completely.Also this study showed light on the inhibitory effect of probiotics suspensions against the adhesion property of S.pyogenes after treated with L.acidophilus and L.fermentum.where the Result shown that L.acidophilus inhibit the adhesion of S.pyogenes with percent 68.92% either L.fermentum inhibit the adhesion of this pathogenic bacteria to epithelial cell with percent 30.28%. The study is consisted of showed the inhibitory action for suspensions of L.acidophilus and L.fermentum on biofilm formation by S.pyogenes, it was noticed the action of L.acidophilus in the side more than from the action of L.fermentum. During the study of the effect of suspension and filter of L.acidophilus and L.fermentum on the production of Hemolysin enzyme by S.pyogenes, it was noticed that the ability of suspension belong to L.acidophilus and L.fermentum to reduce the diameter of hemolysis zone which was beta type more than the ability of filter L.acidophilus and L.fermentum to reduce of the hemolysis zone which was beta type.

توصيف العاثيات الحالة للمكورات العنقودية الجلدية (S. epidermidis) Characterization of Lytic Phage Against Staphylococcus Epidermidis

اسم المؤلف: ريام سليم هندي الزيادي
اسم المشرف: غانم عبود جابر المولى
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: لحصول على عزلات Staph. epidermidisتم جمع (160 عينة) من حالات سريرية مختلفة كالتهاب المجاري البولية, التهابات جلدية, البلعوم, الانف, والاذن. تم الحصول على 51 (875¸31?) عزلة للمكورات العنقودية الجلدية Staph. epidermidis خلال فترة امتدت من ايلول 2014 الى كان | In order to isolate Staph. epidermidis, 160 clinical specimens were collected from (urinary tract infection, skin infection, pharynx, nose, and ear). Only 51(31.875 %) Staph. epidermidis isolates were obtained during a period from September 2014 to January 2015, depending on some biochemical tests and VITEK2 system. The Staph. epidermidis was given (gram stain, catalase , urase) positive, (coagulase, manital fermentation, oxidase, motility) negative, non haemolytic to human blood with some exception, most of the strain were able to produce biofilm, and (100%), (64.70%), (74.50%) resistant to penicillin G, ampicillin, and amoxicillin respectively. The Staphylococcus species identified during study were 44 (27.5%) isolates of Staph. aureus, 5 (3.125 %) Staph. haemolyticus, 4 (2.5 %) Staph. saprophyticus, 8 (4%) isolates other Staphylococcus spp. such as Staph. hominis ssp. hominis, Staph. capatis, Staph. xylose, Staph. simulanus, Staph. lentus, and 48 (30 %) from unknown genera. To isolate bacterioghages from sewage water, several sewage water samples were assayed using plaque assay of double agar overlay as a source of Staph. epidermidis phages. The bacteriophages were described depending on plaques size and shapes, phage 1 was the most predominant and frequent in the bacterial lawn and able to infect other S. species such as S. aureus. It has been selected to study it is titer, latent, rise period, and burst size were calculated. The effect of temperature, pH , and NaCl ions on it is original titer were studied.The results revealed a gradual decreasing in the phage titer with increasing dilution number. Latent period extended to (30 minutes) , while rise period was started with (40 minutes) extending to (60 minutes) , burst size was 2.346. Each temperature at several incubation periods , pH , and NaCl ions was significantly varied depending on phage titer. The optimum temperature was 40 ° C, while the 80 ° C was represented the inhibitor temperature. L.S.D. at level (0.05) for interaction was 39.552. The pH 6.5 - 7.5 represented the optimal pH for the best phage activity while the phage titer beginning to declining in above and below this range of optimal pH, L.S.D. at level 0.05 was 17.898 , the optimum NaCl ions concentrations were (0.1 M and 0.25 M), while the titer was significantly decreased with increasing the NaCl ion concentration in the culture solution, the L.S.D. at level 0.05 was (10.696). In conclusion of this study found that Phage1 was considered as predominant phage because of their ability to infect other Staphylococci species such as Staph. aureus.

دراسة تصنيفية لبعض مراتب الجنس Ranunculus L. (Ranunculaceae في العراق A Taxonomic Study of Some Taxa of The Genus Ranunculus L. (Ranunculaceae) In Iraq

اسم المؤلف: علي طالب محمد الطائي
اسم المشرف: مازن نواف عبود العاني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: “The current research dealed with fourteen taxa belonging to the genus Ranunculus L., Family Ranunculaceae that grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also were studied and the chemical compounds (Flavonoids), Ecological and geographical distribution and taxonomic treatment have been done. Also botanical key was puted to separate the Taxa”.“General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens in the Iraqi herbaria were studied after ensuring that it was correctly identified”. “Comparative morphological study was undertaken to all plants parts of taxa including the roots, leaves, stipules, flowering parts and fruiting parts. Charts, diagrams, tables were drawn and accompanying with natural images for different morphological characteristics, as well as the field images that are important for the study. This study showed that all the taxa of the genus were herbs that are annual or perennial. There was agreat important for the roots, stems and there Indumentum, the presence orabsence of stipules, shape and size and margin of basal leaves and cauline leaves and leafy bract, the shape of sepals and their numbers and their Indumentum, the numbers and shape of Petals and their colors, the number of Stamens and the colors of Anthers, the shape of ovary and their indumentum, presence or absence of style as it was very useful for the taxonomic identification and separation of the studied taxa. As well as the fruiting parts including the length of fruiting peduncle, the fruiting receptacle, the fruiting head and its shapes and their dimensions, the number of Achene Fruitlets and their sizes, colors and Beak shape. Also the surface configuration of the fruitlets Surface has been studied by (SEM) as it wae either hairy or tuberculate - hairy or Transversely rugose or muricate or reticulate or reticulate - minutly rugose or granular or granular - hairy or granular - hairy tuberculate or spinulose”.“The study of the micro - morphological characters of hairs, leaves epidermal cells and the stomatal complex, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa”.“The study show that the pollen grains have a taxonomic importance because of their difference in shape, dimensions, the wall thickness and their ornamentation. It was either apolar which was spheroidal and pantoporate, or isopolar which has different shapes in the equatorial view it was spheroidal, subprolate and oblate - spheroidal, it was either 3 - colpate, 4 - colpate or 5 - colpat.the colpate where either zonocolpate or syncolpate in the form of parasyncolpate. The ornamentation were verucate, echinateand scabrate”. “The chemical study also has an importance in separation of the genus taxa, six flavonoid compounds were diagnosed by using” HPLC wich are Kaempferol, Vitexin, Orientin, Quercetin, Iso - vitexin and Iso - orientin. According to the presence or absence of those structures cluster analysis where done for the results of the flavonoid compounds and the taxa where divided into groups that reveal the chemical relationships between them.dendogram where performed which also show the chemical relationships between the taxa by using UPGMA method and NTSYS program issue 21. the study shows that the presence and distribution of those compounds vary according to the variation in the plant distribution the study shows that some of the taxa have “the same compounds which enhance its belonging to the Ranunculus genus”. “Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical. Most of the taxa were distributed in N. and N.E of Iraq regions districts especially in MAM, MSU, MRO, and FBF districts. We notice that MSU district has a great number of the plants under the study as we found there are all the taxa accept one”. This study helps to detect distribution areas for some taxa that have been not mentioned in the Iraqi flora or other records which are R.kotschyi and R.trichophyllus var.trichophyllus. Also the study records distribution for the new species R.chius in MAM district which hasn't been mentioned in the Iraqi flora in this district before. Finally all the taxa were classified and the botanical key was done to separate them.

تقييم التعبير الجيني للجينات CK19, MGB, MUC1 microRNA - 195 and microRNA - let 7a في نساء عراقيات مصابات بسرطان الثدي Evaluation of MGB1, CK19, MUC1, microRNA - 195 and microRNA - let - 7a Expression In Iraqi Women With Breast Cancer

اسم المؤلف: جودت نوري غائب
اسم المشرف: عبد الحسين مويت الفيصل
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aimed to shed light on the identification a panel of genes with distinct expression patterns in breast cancer patients as a useful tool for breast cancer early detection and progression. The present study designed to investigate the levels of genes expression of five genes panel (MGB1, CK19, MUC1, miR - let7a, and miR - 195) in circulating free mRNA and miRNA from blood of breast cancer patients versus noncancerous samples (benign tumor and healthy controls) to establish a biomarker panel potentially useful for early detection and progression of disease. The expression patterns of the identified genes were then compared with certain clinical features (age, lymph node status, and tumour size).Blood samples from 55 patients with different stages of newly diagnosed invasive ductal carcinoma were provided by certain Iraqi hospitals. Two control groups were used in this study; 10 samples of patients with benign breast tumors, and 20 samples from healthy donors. The samples preservation with TRIzol was done. Samples subjected to total RNA extraction, and then molecular study by using reverse transcription and real time PCR at Molecular Oncology Unit in Guy´s Hospital - Kings College / London.The study reached the following results : 1. The patients’ age range was 24 - 70 years and the median was 49 years with high frequency of patients in the range of 40 - 59 years. According to the family history, 50(90.91%) of patients were have negative family history. According to the clincopathological features (lymph node status and tumor size) the percentages of patients with multiple lymph nodes and tumor size 2.0 - 2.9 cm were the highest groups, which showed statistically highsignificant differences.2. For MGB1 gene expression, the result showed that 30(54.5%) patients were MGB1 - positive while 25(45.4%) patients were MGB1 - negative.According to malignancy status the percentage of patients with high level of MGB1 gene expression 22(40%) was significantly high. In correlation with age groups there was statistically no significant differences in the levels of gene expression with age. In correlation with clincopathological features, lymph node status showed that the highest percentage of MGB1 positive patients 18(66%) were multiple for lymph node status, and the tumor size results showed that there was decreasing in the MGB1 geneexpression with increasing of tumor size. 3. For CK19 the results of present study showed that 41(74.54%) patients were CK19 - positive, while 14(25.46%) patients were CK19 - negative.According to malignancy status the percentage of patients with high level of CK19 gene expression 30(54.45%) was significantly higher in compare with benign tumor patients and healthy controls. In correlation with age groups there was statistically no significant differences in the levels of gene expression with age. In correlation to the clincopathological features, lymph node status results showed that the highest percentage of CK19 positive patients 24(88.89%) were multiple for lymph node status, and there was increasing in the CK19 gene expression with increasing of tumor size.4. Mucin 1 (MUC1) gene expression results showed that the percentage of MUC1 - positive breast cancer patients 72.73%(n=40) was significantly higher when compared with benign tumor patients and healthy controls. According to the age groups the results showed no significant correlation with patients age groups. The clincopathological features results showed that the highest percentage of MUC1 positive patients 84.21%(n=16) have few lymph node status, and there was statistically significant association between the increasing of MUC1 gene expression and tumor size. 5. The miR - 195 gene expression results showed that the percentage of patients with positive miR - 195 gene expression 83.64%(n=46) was significantly higher than patients with negative miR - 195 expression 16.36%, the study also showed that the percentage of high miR - 195 expression samples 69.09% (n = 38) was significantly higher in compare with benign tumor patients and healthy controls. According to the clincopathological features, patients with multiple and few lymph node metastasis were found to have significantly the highest percentages of miR - 195 expression, while the tumor size results showed that there was increasing in the miR - 195 gene expression with increasing of tumor size. 6. The percentage of miR - let 7a - positive breast cancer patients 81.82% was significantly higher, when compared with miR - let 7a - negative patients 18.18%. In correlation to the clincopathological features, results showed no significant correlation in miR - let7a gene expression levels with patients age groups, for lymph node status, the results showed that the highest percentages of let 7a positive patients were those with multiple lymph node and few lymph node metastasis. The tumor size results showed that there was increasing in miR - let 7a gene expression with increasing of tumor size.7. According to genes combinations, three genes combination (CK19, miR - 195 and miR - let 7a) was significantly positively expressed with percentage of 60%(33/55), which reflect their potential diagnostic and prognostic value.8. The study concluded that the three genes combination (CK19, miR - 195 and miR - let 7a) may have potential applications as a diagnostic and prognostic markers for breast cancer.

تاثير بعض المستخلصات النباتية في معايير بيولوجية مختلفة للفئران Effect of Some Plant Extracts On Different Biological Parameters On Mice

اسم المؤلف: عقيل حيدر عطا الله
اسم المشرف: مؤيد صبري شوكت
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية للتحري عن المركبات النشطة الموجودة في المستخلص الخام المائي والميثانولي لاوراق الاس والنعناع والريحان ودراسة تاثيرها على فعالية انزيم الاسيتل كولين استريز ومستوى الدهون ومستوى السكر ومستوى انزيمات الكبد(ALP وSGPT وSGOT) ومستوى الانتر | The present study was conducted to investigate the active constituents found in aqueous and methanolic crude leaf extracts of Myrtus communis, Mentha piperita and Ocimum basilicum, and studies it effect on the acetylcholinesterase activity, levels of lipids, level of glucose, level of liver enzymes (ALP, SGPT and SGOT) and level of Interleukins (IL - 2, IL - 4, IL - 6 and IL - 10) in laboratory mice (in vivo). The results of the phytochemical analysis of the crude aqueous and methanolic extracts of Myrtus communis, Mentha piperita and Ocimum basilicum contain active compounds : Phenols, Flavonoids and Tannins and missing of Steroids and Coumarines in all extract but Saponins and Alkaloids found in alcoholic extract only, while terpens were present in Mentha piperita and Ocimum basilicum and absent in Myrtus communis. The results of administrating animals with different extracts showed no significant difference on blood Acetylcholinesterase (AchE) compared with ethanol liquid diet, while the alcoholic and aqueous extracts of M. communis, M. piperita and O. basilicum in the serum of decreased Acetylcholinesterase level significantly(p?0.05), liver and brain [(1.25 ?pH/30 min, 1.23 ?pH/30 min, 1.28 ?pH/30 min, 1.20 ?pH/30 min, 1.26 ?pH/30 min, 1.28 ?pH/30 min), (0.35 ?pH/30 min, 0.34 ?pH/30 min, 0.34?pH/30 min, 0.36?pH/30 min, 0.42?pH/30 min, 0.39?pH/30 min), (0.32?pH/30 min, 0.37?pH/30 min, 0.39?pH/30 min, 0.36?pH/30 min, 0.34?pH/30 min, 0.37?pH/30 min)] respectively compared with animals fed on ethanol liquid diet [(1.37 ?pH/30 min), (0.47 ?pH/30 min), (0.45 ?pH/30 min)] respectively. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum reported a significant decrease in level of the total cholesterol and Low Density Lipoprotein (LDL) [(181mg/dl, 186mg/dl, 175mg/dl, 172mg/dl, 181mg/dl, 184mg/dl), (118mg/dl, 121mg/dl, 114mg/dl, 109mg/dl, 118mg/dl and 120mg/dl)] respectively, when compared with animals fed on ethanol liquid diet [(195 mg/dl), (132mg/dl) ] respectively while no effect was reported on High Density lipoprotein. The level of triglyceride reduced after the treatment with ethanol liquid diet, and then the level increased after the treatment with M. communis and O. basilicum extracts. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum have led to a reduction in the level of glucose in the serum which increased after the treatment with ethanol liquid diet. Methanolic and aqueous extracts decreased the liver enzymes (ALP, SGPT and SGOT) significantly to the normal level (18, 17, 21, 18, 20 and 21) KAU, (19, 19, 16, 13, 17, 17) IU/L and (21, 24, 17, 15, 17 and 19) IU/L respectively after the increase by the treatment with ethanol liquid diet (24) KAU, (26) IU/L, (28) IU/L as compared to control (20) KAU, (12.2) IU/L, (13.5) IU/L, respectively. The level of Interleukin - 2 and Interleukin - 4 in the serum significantly increased in the treatment of alcoholic and aqueous extract of M. communis, M. piperita and O. basilicum [(18 pg /ml, 17 pg /ml, 20 pg /ml, 18 pg /ml, 20 pg /ml, 20 pg /ml), (100 pg/ml, 110 pg/ml, 119 pg/ml, 108 pg/ml, 90 pg/ml, 92pg/ml)] in comparison with ethanol liquid diet treatment [(14 pg /ml), (77 pg/ml)] respectively. While the level of Interleukin - 6 and Interleukin - 10 increased significantly in the serum, when animals were fed with ethanol liquid diet [(259pg/ml) and (501pg/ml)] respectively, and then decreased significantly after the treatment of methanolic, aqueous extract of M. communis, M. piperita and O. basilicum reported [(198 pg/ml, 202pg/ml, 202pg/ml, 201pg/ml, 214pg/ml, 217pg/ml), (370 pg/ml, 385pg/ml, 200pg/ml, 280pg/ml, 350pg/ml and 350pg/ml)] respectively.

دراسة تصنيفية حياتية مقارنة لمراتب من الجنس Onobrychis Mill. (Fabaceae) في العراق

اسم المؤلف: زينب جواد عامر
اسم المشرف: عذية ناهي سلمان المشهداني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استــهدف البــحث الــحالي دراســة تصــنــيفية مقارنة لاحدى عشر مرتبة تصنيفية تابعة للجنس Onobrychis Mill. من العائلة الفراشية (البقولية) Papilionaceae النامية بريا في العراق وهي O. acaulis وO. caput - galli وO. carduchorum وO. crista - galli بضربيه O. cri | A comparative systematic study was carried out for eleven taxa belong to the genus Onobrychis Mill., Family Papilionaceae grown widely in Iraq, and they are O. acaulis, O. caput - galli, O. carduchorum, O. crista - galli with two varieties O. crista - galli var. crista - galli and O. crista - galli var. trilophocarpa, O. galegifolia, O. haussknechtii, O. kotschyana, O. megataphros, O. ptolemaica, O. schahuensis. The Study included the gross general Morphological characters and Micromorphological characters, also the Pollen grains studied and Anatomy, Chemistry, Enviroment and Geographical distribution and Taxonomic treatment, also botanical key was putted to separate the taxa. General survey for the Phytogeographical districts where the taxa of genus distributed, in order to detect the distribution of the studied taxa of the genus Onobrychis. Therefore many collections obtained and new locations of taxa distribution were recorded, the specimens studied, and correct the mistake naming specimens were identified and reidentified some miss identified specimens. A comparative morphological study was undertaken to all taxa plants parts include the root, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying, with natural images for different morphology characteristics, as well as the field images. This study indicates that the characters of Leaf, Inflorescence, Flowers and Fruit were collectively contributed in the separation and identification of taxa. The pollen grains have a taxonomic importance because their different shape, dimensions of polar and equilateral view and wall thickness. The anatomical study showed the importance of Taxonomic support to the morphological characters in the diagnosis and isolation different taxa, and this study showed different in the anatomical characters of leaf include the characters of Epidermis, Stomata and the characters of a cross sections in stems. The current study showed that the trichomes were very important taxonomic in isolate different taxa because their variation in density, dimensions and the shape of epidermis cells that the trichomes arise from them and dimensions of the epidermal cells and the nature of their walls. The current study identified nineteen protein - amino acids by using High - Performance Liquid Chromatography (HPLC) and the distribution of these compound was variable among various taxa of the genus and that was useful in isolation different taxa. and the aliphatic amino acids, Glutamic acid, Glycine, Serine, Arginine, Alanine, Tyrosine, Valine, Methionine, Isoleucine and Lysine had a wide presence in the studied taxa, which means that they belong to the same genus. The ecology and geographic distribution of the taxa were studied using area survey and information of herbaria specimens, maps have been drawn for taxa distribution in the Iraqi natural geographical provinces. At last, a taxonomic treatment has been carried out for the taxa of the genus Onobrychis, as well as a detailed key to isolate them.

دراسة عدد من ملوثات الهواء وتاثيراتها البيئية في منطقتين مختارة من جانب الرصافة في بغداد Study of Several Air Pollutants And Their Environmental Effects In Two Regions In Al - Rusafa Site In Baghdad

اسم المؤلف: شيماء هادي حمادي الدليمي
اسم المشرف: محمد نافع علي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Several air pollutant concentrations such as Suspended Particulate Matter (SPM), carbon oxides (COx), sulfur dioxide (SO2), nitrogen oxides (NOx), methane (CH4), ozone (O3) and some heavy metals such as lead (Pb) , chromium (Cr), cadmium (Cd), and nickel (Ni). Were measured in selected areas of the Rusafa in Baghdad, Anda’lus square (as a commercial site) and Wazeria district (as industrial site).The results showed that the concentration of air pollutants from gases, SPM (NO2, CO, SO2, and SPM) and heavy metals (Pb, Cr, Cd, and Ni) were exceeded globally and locally permissible limits in both study sites, while the concentration of O3, CO2 were within the globally and locally standard limits in both study sites. Commercial site had recorded higher concentrations of (CO2, SPM, Pb) than those of industrial site. While the industrial site had more pollution with (NO, NO2, CO, CH4, Cr). Results showed a relationship between climate factors and air pollutants, where windspeed, temperature, and relative humidity had influenced on the concentration of air pollutants.Median Lethal Concentration (LC50) to nitrogen dioxide gas was identified, by exposing a community of 30 adult male mice. The mice weights were between 23 - 31gm and their age was 8 - 12 weeks. The mice were randomly divided into five groups (6 male of each group). The exposure periods were 20, 40, 60, 80, 100 minutes using different concentrations 50, 100, 150, 200 ppm.The results showed a higher mortality during 150 and 200 ppm concentrations, for exposure periods of 80 and 100 minutes, but other concentrations have not been recorded. LC50 was calculated 161.86 ppm for 80 minutes and 110.53 ppm for 100 minutes.

دراسة التاثيرات الحادة والمزمنة للمبيد العشبي - 4, 2 دايكلوروفينوكسي اسيتك اسد في نوعين من اسماك الكارب Study The Acute And Chronic Effects of The Herbicide 2, 4 - Dichlorophenoxy Acetic Acid In Two Species of Carp Fish

اسم المؤلف: ضحى زكي السويفي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية التاثيرات السمية الحادة والمزمنة لمبيد 2, 4 - d في نوعين من اسماك الكارب (الاعتيادي والعشبي)، استعمل في دراسة التاثيرات الحادة عدة لكلا النوعين قيد الدراسة وكانت ((170, 165, 160, 155, 145, 140, 130 و(140, 130, 115, 95, 70)ملغم/لتر ع | This study included acute and chronic effects of 2, 4 - d pesticide in two species of carp fish (Cyprinus carpio and Ctenopharyngodon idella) using several concentrations for the two carp fish species that were (130, 140, 145, 155, 160, 165, 170) and (70, 95, 115, 130, 140) mg/l respectively for 96 hours, to determine the median lethal concentration (LC50) and the behavioral changes was used as a standard of those effects. Whereas three concentrations (20, 40 and 60) and (10, 15, 25) mg/l respectively for the two carp species were used in the chronic exposure for six weeks. The behavioral, histological and hematological changes were used as a standard to observe these effects through the study period. The value of LC50 for common carp fish were 157.77, 157.25, 156.41 and 152.62 mg/l. For grass carp, LC50 were 115.92, 114.92, 112.94 and 96.52 mg/l for 24, 48, 72, 96 hrs of exposure to 2, 4 - d pesticide concentrations respectively.Behavioral changes were recorded more effects in high concentrations than in low concentrations. To discover the toxic effects of this substance on fish, many parameters were used. They included the clinical symptoms by erratic swimming, hyperactivity, loss of equilibrium with respiratory effects such as rapid gill movement and swam near the water surface. While in chronic effects, blood parameters were changed in Red Blood Cell R.B.C., White Blood Cell W.B.C., Packed Cell Volume P.C.V., Haemoglobin Hb and liver enzyme (GPT & GOT).Values elevated significantly according to the pesticide concentration (P?0.001) and exposure periods (P?0.05) in comparison with control. The histological examination of the gills, liver and kidney of the common and grass carp fish after chronic exposure to 2, 4 - d herbicide showed pathological changes and alterations such as fusion of the secondary lamella, epithelial lifting, blood congestion, epithelial hypertrophy of the lamellar epithelium and epithelial necrosis. In the liver, pathological changes were seen, including hepatocytes hypertrophy, cytoplasmic vacuolation, blood congestion and cellular necrosis. Degenerative changes of the renal epithelium, necrosis in renal tubules and occlusion of the tubular lumen where the pathological alterations in the kidney.

انتشار داء المقوسات العيني مع تقييم لبعض جوانب الحالة المناعية في عينة من المرضى العراقيين Prevalence of Ocular Toxoplasmosis And Evaluation of Some Immune Status Aspects In A Sample of Iraqi Patients

اسم المؤلف: عبير فاروق ساكن الحكيم
اسم المشرف: اسراء قاسم العبيدي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على 104 مريضا من المراجعين لوحدة الشبكية في مستشفى ابن الهيثم التعليمي للعيون في بغداد للمدة من بداية شهر كانون الاول 2013 لغاية شهر حزيران 2014. قسمت مجموعة المرضى استنادا للتشخيص السريري وفحص اختبار التلازن Latex test الى 50 مصابا | The current study was conducted on 104 patients whose underwent to the retina unit in Ibn Al - Haitham Hospital of Eyes in Baghdad from December 2013 to May 2014. The patients groups were divided according to the clinical diagnosis to 50 patients infected with ocular toxoplasmosis (OT) and 54 patents with uveitis, in addition to these groups acontrol group was also included 96 healthy apparent persons without any infection in their eyes.Some epidemiological characters were studied like age, that showed a little effect on two groups OT (Mean of age 38.07 ± 15.14) and uveitis (Mean of age 35.1 ± 17.6). The two groups were found in range 20 - 40 year while the control group include individuals with different age due to the randomly collection. The occupation factor play arole in the prevalence of infection with highly significant differences (P<0.05) in (house wife, workers) with a percentages 35(70%) 35(64.8%) in OT and uveitis groups respectively but the control group expend to include house wife, workers and officers with a total rate 92.7%.The education level also showed significant differences (P<0.01) among the studied groups, the most of ocular toxoplasmosis and uveitis samples were found in highly frequency at the primary, intermediate and secondary educational level with total rate 74% and 77.81% respectively while the control group was found at the secondary and under graduate educational level in 77.1%. The residency character was also examined and no revealed significant difference (P<0.05).The infection path was also set by the presence of animals especially cats which ware existed leading to statistical differences, p? 0.01 for OT group with rate 18%. The visual activity character showed no significant difference (P?0.05) between the patients of OT and uveitis who affected their visual acuity in right eye while the doubling response was recorded for uveitis patients with rate 55.6% in comparable with OT patients with rate 38% for the left eye. The intra ocular pressure was normal in both groups and for both eyes without significant difference.Latex and ELISA IgG. IgM serological tests were used in this study. The results were showed that latex test is easier and it is appear that OT patients were diagnosis with 100%, while the uveitis patients didn't show any positive result. Control group showed that 21 individuals were infected with rate of 21.9%.ELISA - IgG toxo. antibodies confirmed that the infection rate of OT patients was 39 (78%) versus 11(22%) with negative result, while the study also confirm that uveitis patient that have OT disease was 12 patient from 54 case, 42 case didn't diagnosis for OT. The control group was re - evaluated showed 28 case with toxoplasmosis infection from 96 case which from the subclinical group. The antibodies levels were measure by international unit/ ml for all groups, the IgG level was 3.186 ± 1.703 Iu/ml in positive control group followed by OT patients with 2.927 ± 2.417 and uveitis group 0.637 ± 0.803, finally the negative control group 0.276 ± 0.163. IgM levels were low and its presence didn't have any positive case. This study also showed that there was a relation between the IgG level and the pathological effect on eyes which resulted from infection for both groups OT and uveitis, it was high rate 73.5% with 50 patient, for posterior uveitis causing significant differences (P<0.05) followed by anterior uveitis with rate 13.2%.As for the results of cytokines there was a high level of IFN - ? in positive control group 793.7 ± 3461.6 pg/ml followed by OT group 550.6 ± 210.6 pg/ml, the uveitis group that in infected with ocular toxoplasmosis and negative control recorded 524.1 ± 173.6 and 506.6 ± 104.6 pg/ml levels respectively, this rise in the level of IFN - ? cause a significant differences at (P<0.05) for OT, uveitis (non infected) and positive control groups. TNF - ? recorded arise of its level in positive control group 34.89 ± 12.29 pg/ml followed by OT group 32.24 ± 9.91 pg/ml then positive uveitis group 23.61 ± 12.91 pg/ml after them negative control 14.62 ± 5.43 pg/ml finally negative uveitis group with 7.76 ± 4.04 pg/ml.High level of 1L - 12 was gotin positive control group in a concentration 827.00 ± 224.25 pg/ml followed by OT group with 811.28 ± 179.40 pg/ml. An increase in 1L - 17A was recorded in positive control group with mean 327.65 ± 77.89 pg/ml while converged concentration in OT group 263.73 ± 47.48 and positive uveitis group 252.57 ± 61.55 pg/ml. The lowest level was in negative control group 421.6 ± 168.14 pg/ml. MCP - 1 showed a rise in its level in positive control group 274.09 ± 92.05 pg/ml and reduce in OT patients 217.68 ± 98.70 pg/ml, while the two groups negative and positive uveitis recorded levels 155.71 ± 99.51 and 132.60 ± 99.02 pg/ml respectively. The lowest level was register in negative control 97.56 ± 79.17 pg/ml.

التحري الجزيئي عن بعض التغيرات في الدنا المايتوكونديري للنطف لمرضى يعانون من وهن حركة النطف Molecular Screening of Some Changes In Sperm Mitochondrial DNA In Asthenozoospermic Patients

اسم المؤلف: عدي عدنان مهدي
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من خلال البحوث والدراسات التي اجريت على اسباب وهن حركة النطف لدى الرجال الا انه هناك عدد من المسببات لم تحدد والى الوقت الحاضر. الا انه بالرغم من ذلك بعض الدراسات تشيربان العامل الوراثي يلعب دور في ذلك والمتمثل بعضيات المايتوكوندريا والحاوية على الدنا الم | Through researches and studies done on the causes of the asthenozoospermia in men, although there was no specific reason so far responsible for the infertility in people, but some of the studies were indicated that the genetic factors plays a role in the sperm dysmotility through the mitochondria that contained mitochondrial DNA responsible for providing the energy required for the sperm motility by production of ATP, which serve as the fuel that is consumed by sperm during motility. This study is conducted to determine the changes that could occur in the sperm mtDNA which included (common deletion, mtDNA copy number per cell and mutations in the ATPase8 and ATPase6 genes).This study was consisted of two parts as follow : The first part was consisted of two steps : The first step conducted on 71 samples from subjects suffering from the weakness of the movement of sperm and 12 samples from subjects who have a normal movement of sperm, total samples were 83 samples were divided patient samples into 5 groups depending on the percentage of the sperm motility under the classification adopted by the World Health Organization as well as a control group, that it has become 6 groups, these samples were subjected for investigation about the mitochondrial DNA deletions and mitochondrial DNA copy number by using real time PCR.On the other hand was taking 66 sample dispersed sample 56 patients and 10 healthy samples were subjected to the tests of ATPase6 and ATPase8 genes sequences for detection about mtDNA mutation.The second step was included the use of discontinuous gradient centrifugation (percoll) , which are 40 % and 80 % which represent both progressive and non progressive motile sperms, bringing the total samples 166 samples that collected and fractionated in a hospital, Kamal Al - Samarrai to treat infertility and IVF in Baghdad and examined for further molecular investigations for the period from February 2012 to October 2013.The second part was included the molecular tests that carried out after DNA extraction from the cells and this part include three steps : The first step was to investigate the common deletion in mtDNA where the results showed that the total number of deleted samples in the group (0 - 5) in the nonprogressive sperm was 81.25 %, which ranged between 6.8 - 74.7% while the ratio between the other groups 6 - 10, 11 - 15 , 16 - 20, 21 - 25 where were 75, 35.72, 28.5 , 36.3, and 12% for control group respectively. The second step was to investigate the sperm mtDNA copy number. The same groups where the results showed that the content of DNA in subjects with impaired movement of sperm was high compared with the control group where scored in some samples the highest level of gene expression 246.9 copies per cell in the group (0 - 5%) either other groups have shown lower levels were observed in groups (6 - 10) , (11 - 15) , (16 - 20) and (21 - 25) were 203.1, 180.7, 133.5 and 128.3 copies per cell, either the highest level of DNA content of the control group was 94.7 copies per cell, there was also a significant difference (P>0.05) between the normospermic motile isolated from 80% class and poor sperm movement isolated from 40 % class. The third step was included the detection of mutations by analyzing the sequences of the MT - ATAase8 and MT - ATPase6 genes which documented 59 mutations that were 23 missense and 36 silent mutations where all of them were heteroplasmic mutation except for a single mutation of the type missense (A8860G) which was homoplasmic mutation was noticed in all mtDNA copies of patients and control subjects also in progressive and non progressive sperm cells. Thought this current study novel nucleotides changes in MT - ATAase8 and MT - ATPase6 genes among groups where 3 novel missense mutations in MT - ATAase8 gene at positions (8378, 8483 and 8558), the changes of nucleotides bases were A>G, A>C, A>C and A>C respectively, replacing Asparagine to Aspartic acid, Leucine to Proline and Proline to Alanine respectively. Also two novel missense mutations were observed in MT - ATPase6 gene at positions nt (8822 and 9055) where the changes nucleotides were (C >T and G>A) that replaced amino acids (Serine to Phenylalanine and Alanine to Threonine) respectively. Silent novel heteroplasmic change as a transition substitutions in ATPase8 gene at position nt (8371) by replacing C>T without any changing in amino acid of protein. Results of this present study showed novel heteroplasmic silent mutations in ATPase6 gene at positions nt (8899, 9048 and 9060) nucleotides changes (C>T, T>C and C>A) respectively, without changing in amino acid of protein which were observed in infertile group

تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus

اسم المؤلف: غزوان علي مسلم الرماحي
اسم المشرف: عبد الرضا عبد الحسن اللامي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study includes 82 clinical samples from male patients suspected to have gonorrhea (urethral discharge with dysuria). Two types of samples were collected from each patient, urethra swab and blood samples from patients who attended Al - Yarmouk teaching hospital in Baghdad city, Clinics and private laboratories during a period from December 2012 to April 2013. All of them were married and have children's, and 20 samples were taken from apparently healthy subjects.Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on rich media chocolate agare for growth bacteria and selective media modified Thayer martin agar (MTM) contact antibiotic to prevent growth of all the types of bacteria except N. gonorrhoeae.Microscopic examination using specific kit for gram stain, showed gram negative diplococcus, like bean shape. Culturing on rich media revealed that 82 (100%) samples positive but re culturing on Modified Thayer martin media revealed that 76 (92.68%) out of 82 positive. Then biochemical test had the same results.Results of the molecular diagnosis of the bacteria causing gonorrhea using specific primers that were specific for the Orf1 gene, revealed that 80.26% of samples (61 out of 76) were positive. Results of the microdeletion in Y chromosome AZFc region revealed that no microdeletion were occurred in SY - 254 STS and BPY - 2 gene.This study, provided high specificity and sensitivity for the diagnosis of gonorrhea using PCR technique which is cheaper and faster than traditional methods. Also, PCR - based method for detection of N.gonorrhoeae can be readily used in hospitals and laboratories.

الكشف عن السمية الوراثية لمستخلص نبات الصبار Aloe vera باستعمال المؤشرات الجزيئية والخلوية Genotoxic Effect of Aloe Vera Extract Using Molecular And Cytogenetic Markers

اسم المؤلف: غيث منذر فاضل
اسم المشرف: نضال نعمة حسين
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الوراثة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نبات الصبار Aloe vera L. من النباتات المهمة والتي تستعمل حاليا بشكل واسع في مجال صناعة الادوية ويضاف هلام الصبار الى اغلب مستحضرات التجميل مع بعض المواد الغذائية في جميع انحاء العالم, لذا تم دراسة التاثيرات السمية الخلوية والوراثية لتراكيز مختلفة من المس | Aloe vera L. is one of the important plants in the world.It is currently and widely used in pharmaceutical industry, and its gel in some time added to some foods all over the world. Therefore, the present study was conducted to evaluate the cytotoxic and genetic effects of crude, alcohol and aqueous extracts of Aloe vera gel toward the onion(Allium cepa L.) roots (as a biological system) exposure at different periods. Onions roots were treated with different concentrations of the extracts : 2% , 5%, 10%, 20% and 40% (v/v)of the crud extract , 5%, 10%, 20% 30% and 50% (v/w) of alcohol extract and the concentration 25%, 50%, 100%, 150% and 200%(v/w) of aqueous extract of Aloe vera gel exposed for different period (24, 48 and 72) hour. The investigation were focused on the effect of these extracts on onions roots length rate and some cellular properties (mitotic index, phase index, the percentage and type of chromosome aberrations, as well as a genetic study using Random Amplified Polymorphic DNA (RAPD) of onions roots treated with different concentration of this extracts. Results showed that all Aloe vera gel extracts had inhibit activity toward onion roots length rate. The inhibition associated positively with the increasing concentration. The effective concentration (EC50%) were 10% of the crude extract, 20% of the alcoholic extract and 100% of aqueous extract, indicating the effectiveness of the crude extract followed by alcoholic extract and then the aqueous extract. Cytological study showed that all Aloe vera gel extract revealed a significant decrease in mitotic index (MI%) of the onion roots cells compared with the control group, and the effect was positively associated with the increasing concentrations of the extract. However, the effect was independent on the exposure period. The concentrations 10%, 20% and 150% of the crude, alcohol and aqueous extract respectively indicated a reduction of the mitotic index to almost 50% of the control treatment. Therefore, this concentrations considered as sub - lethal concentrations for this extracts respectively while the higher concentrations than 30%, 50% and 200% of the crude, alcohol and aqueous extract which reduced the mitotic index to almost 22% of the control treatment were considered as lethal concentrations. The results also showed a significant decreases in prophase and an increase in metaphase in onion roots cells treated with extracts comparison with control treatment. Many of chromosomal abnormalities were appeared due to the treatments of the onion roots with different concentration of the extracts, the percent abnormalities was increased with the increasing concentration of the extract and exposure period. The most frequent of chromosome abnormalities were chromosomal stickiness, disturbed chromosome, chromosomal bridges, C - mitosis, vagrant chromosomes in addition to the other chromosomal abnormalities star telophase. The toxicity effect of Aloe vera gel were studded on molecular level by using random amplification polymorphism of DNA(RAPD). Ten random primers were used, seven of them gave polymorphic bands in all the studied samples and their molecular weights were ranging from 100 to 1600 base pair. The results of Random Amplified Polymorphic DNA (RAPD) showed significant difference in the number of DNA band and their molecular weight of the onion roots treated with Aloe vera extracts compare to control treatment. The high concentrations(higher than EC50%) of extracts were showed to be more effective on the DNA through the appearance or disappearance of larger number of DNA band in comparison with control. The genetic template stability (% GTS) was significantly decreased in DNA of onions root treated with high concentrations (higher than EC50%) of Aloe vera extracts, therefore the high concentrations of Aloe Vera extracts were considered to be genetically toxic. The genetic dendrogram was obtained using Jaccard factor of genetic divergence and the result showed segregation of the samples treated with heights concentrations (%40 200% and 50%) of crude, alcohol and aqueous extract from other samples and control treatment. Therefore, the RAPD method can be considered as an efficient method in detecting for assessment of genotoxicity of crude, alcohol and aqueous extract of Aloe vera gel.

التفكك الحياتي لمبيدي الملاثيون والدورسبان باستخدام مزارع احاديه وخليطه من البكتيريا Biodegradation of Malathion And Dursban By Mono And Mixed Bacterial Cultures

اسم المؤلف: فائزة كاظم عمران
اسم المشرف: عادل مشعان ربيع
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: عزلت 45 عزلة بكتيرية محليه من مجموع 30 نموذج تربه ملوثة بالمبيدات والتي جمعت خلال فترات زمنيه مختلفة ومن مناطق مختلفه في بغداد. اختبرت قابلية العزلات البكتيريه على تفكيك مبيدي الملاثيون والدورسبان. اجريت غربلة اولية وثانوية باستخدام وسط الاملاح المعدنية ا | Forty five local bacterial isolates were isolated from thirty different soil sample contaminated with pesticide, These samples were collected from different Baghdad regions at different periods. The bacterial isolates were tested for their ability to biodegrade certain pesticides (Malathion and Dursban). Primary and secondary screenings were carried out using solid Mineral Salts Medium (MSM) and Liquid (MSM) with 100 ppm Malathion and Dursban at 37 0C, pH 7. Twenty seven isolates resistant to Malathion and Dursban were isolated. Out of 27 bacterial isolates from different locations in Baghdad. Results from primary screening showed that the 10 isolates had good growth, 5 isolates gave moderate growth, 12 showed weak growth and only 9 isolates failed to show any growth.According to current results, 10 bacterial isolates were tested for their ability to biodegradation of Malathion and Dursban in liquid medium, However, secondary screenings results by using minimal inhibition concentration(MIC) revealed that only four bacterial isolates had the highest ability to degrade Malathion and Dursban and subsequently were identified as Pseudomonas putida, Esherichia coli, Escherichia hermanii and Staphylococcus vitulinus by Vitek compact system respectively.The optimum conditions (incubation period, pH, temperature) for growth and biodegradation of Malathion and Dursban were examined. The obtained results indicated that the best incubation period was after 7 days, pH =6 and favorite temperature was 350C.Measurement physicochemical properties for soil pH, temperature (C°), electrical conductivity EC (µs/cm) for both Dursban and Malathion concentrations(ppm) collected from six different locations within Baghdad city. Biodegradation of Malathion and Dursban in soil inoculated with different bacterial mono - cultures and mixed - cultures were investigated. soil pH, temperature, electrical conductivity, cations and anions, on the population of native Malathion - Dursban tolerant bacteria in the cultivated soils of six sites. The result showed that soil physical properties like temperature and electrical conductivity affect significantly to native Malathion - Dursban tolerant bacterial density. Similarly, the soil chemical properties like pH, cations and anions had more effect on the abundance of Malathion - Dursban tolerant bacteria in the soil. However, the impact of soil organic carbon, organic nitrogen and available phosphorus was very significant. The results of the present study can be utilized for the development of effective bioremediation process for pesticide - contaminated soil. Under optimal conditions, Malathion and Dursban degradation was measured by using GC - MS analysis. The results revealed that Pseudomonas putida was the best isolate for degrading Dursban by monoculture isolate (47.18%) , while mixed culture(P.putida and S.vitulinus) was the best in degrading Malathion and Dursban that gave 90.84% and 81.055% respectively. Results also indicated that the number of byproducts produced by mono and mixed culture of Malathion and Dursban biodegradation. Whereas the results obtained from GC/Ms analysis revealed that not detected of toxic byproduct Malathion and Dursban degradation by Pseudomonas putida and Staphylococcus vitulins respectively.Moreover, under natural conditions.The study tested the ability of selected bacterial isolates (P. putida and S. vitulins) in biodegradation of Malathion and Dursban, the results showed slower biodegradation by selected bacterial isolates compared with laboratory conditions but study showed a intermediet product like phenol, diethyl phosphorothioate and oxon.The GC - MS results showed that the number of metabolic intermediates formed during the degradation of Malathion by bacterial mixed - culture were relatively higher in number than by bacterial mono - culture.The mass spectra of malathion containing samples treated with bacterial mono and mixed culture showed no any known toxic intermediates. The result indicated that the two main degradation products resulted from bacterial degradation, namely malathion monocarboxylic (MMA) and malathion dicarboxylic acid (MDA), the first one may convert into the latter over time. Some other degradation products may occur such as ethyl hydrogen fumarate (EHF) but in negligible amount.The result indicated that the main degradation products resulted from bacterial degradation of dursban is trichloropyridinol(TCP) and then de - chlorinated into 2 - pyridinol.The mass spectra results obtained in this study showed that the dursban was degraded to many metabolites.The results suggested that the bacterial isolates were not forming any toxic intermediates but no any intermediate was identified till R.T. 22 minutes. This indicated that dursban is probably completely metabolized by the isolates into smaller intermediatesThe isolate Pseudomonas putida isolated from soil contaminated with pesticides was identified genotypic tests. Molecular typing was performed by RAPD - PCR and comparison of the results to other Pseudomonas isolates. The result shows that genotyping differences between isolates and found the convergent percent biodegradation between this isolates of both selected pesticide, although from these differences, there was no more effect to biodegradation, which means that the biodegradation of Malathion and Dursban not related to genotype

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

اسم المؤلف: قاسم ابراهيم حسين
اسم المشرف: ايمان ناطق ناجي البياتي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

تحديد شدة الاصابة الحادة والمزمنة لداء المقوسات الكوندية بواسطة مستويات (IL - 6, IL - 8, TNF - ?) Determination of Acute And Chronic Toxoplasmosis By Estimation of IL - 6, IL - 8 And TNF - ? Levels

اسم المؤلف: لينا قاسم كاظم الزهيري
اسم المشرف: بان نوري القاضي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقوسات الكونديه من الطفيليات الشديده النجاح في انشاء اصابة مزمنه طويلة الامد. وقد اجريت الدراسة الحاليه على 366 امراة (117 مجهضات، 141 حوامل، 108 غير متزوجات) جمعت من مستشفيات مختلفة في بغداد. جمعت عينات الدم لجميع المجاميع المدروسة خلال الفترة مابين تش | Toxoplasma is a highly invasive parasite which establishes a life - long chronic infection. The present study was performed on 366 women (117 miscarriages, 141 pregnant and 108 singles) aged (17 - 35) years old from different hospital in Baghdad. Blood samples were collected from all patients during the period of Oct.2013 until of Jan. 2014. Anti - toxoplasma antibodies in the sera of all samples by using two tests, Latex test (LAT) and ELISA (IgG), (IgM) while, the proinflammatory cytokine were estimated by evaluation of the cytokine such as (IL - 6, IL - 8, TNF - ?). The results were showed that : ? The infection rate of toxoplasmosis by LAT was (41.53%) which was more sensitive than ELISA IgG (36.85%), and miscarried women were diagnosed by higher anti - toxoplasma IgG antibodies 62/117(52.99%), while pregnant result were lower 30/108 (27.78%). ? The most frequent age group of chronic infection with T.gondii was (23 - 28) year in both miscarriage and pregnant women, and represented by 45.16%, 40% respectively. While the most frequent age group for a single woman was (17 - 22) years, and represent 46.511% of the total number of this group.? Seroprevalence of toxoplasmosis by ELISA IgM test revealed low sensitivity in different studied groups 25/366(6.830%), and miscarried women were diagnosed by a highly significant (P ?0.01) percentage of acute toxoplasmosis14/117(11.965%).and The age group of (17 - 22) years old diagnosed high significant (p?0.01) percentage of acute toxoplasmosis in all studied groups.? According to the distribution of infected miscarried women with toxoplasmosis and gestational age by ELISA IgM the result showed that most of miscarriages were occurred at the first trimester which considered high significant (p? 0.01) 8/14(57.42%). While, chronic infected women at the second trimester (4 - 7 month) diagnosed by significantly (P?0.01) higher percentage of miscarried 24/62(38.71%).? The level of pro - infalmmatory cytokine (IL - 6) in acute infected miscarried women with toxoplasmosis distincted with a significantly high increase (P? 0.05) (177.31±12.53 Pg/ml) in comparison to chronic infected one (119.36 ±14.07 Pg/ml).While, chronic infected pregnant women was low significantly (P ?0.01) (48.41± 2.79 Pg/ml) in comparison to acute infected one and other studied groups, ? The acute infected singles was (118.98± 15.68 Pg/ml) higher non - significantly (P?0.05) than chronic infected one (112.65 ± 17.91 Pg/ml) and both disease activity were significantly higher than its level in healthy singles.? The level of TNF - ? in acute infected miscarried women distincted with significantly higher (P?0.05) (97.46 ± 5.37 Pg/ml) in comparison to chronic infected women (69.55 ± 4.58 Pg/ml), Whereas the mean level of TNF - ? in pregnant infected women during acute phase (62.31 ± 3.82 Pg/ml) was significantly (P?0.05) higher than chronic infection (21.42 ±2.74 Pg/ml)? Single infected women showed high significant increase (P?0.05) of TNF - ? level (50.48 ± 7.42 Pg/ml) in comparison to chronic infected one (35.46± 5.36 Pg/ml) and both disease activity scored high significant increase (P?0.05) of TNF - ? in comparison to healthy singles (18.29 ± 1.02 Pg/ml).? Finally, the mean level of IL - 8 in miscarried women with positive infection with T. gondii during acute phase (187.92 ± 15.38 Pg/ml) was significantly (P?0.05) higher than chronic phase of infection (140.57 ± 13.87 Pg/ml).Whereas the mean level of IL - 8 in acute infected pregnant women (110.32 ± 9.13 Pg/ml) was higher significantly (P?0.05) than healthy pregnant (91.49 ± 7.59 Pg/ml) and healthy singles (88.82 ± 5.62 Pg/ml).? Infected single women showed highly significant (P?0.01) increased levels in both diseases activity in comparison to single women without infection (88.82± 5.62 Pg/ml).

دراسة التاثيرات السمية للـ Gliotoxin المنتج من العفن Aspergillus fumigatus باستخدام تخمرات الحالة الصلبة Study Toxicity Effects of Gliotoxin Produced By Aspergillus Fumigatus Using Solid State Fermentation

اسم المؤلف: محمد عادل نوري
اسم المشرف: عبد الكريم جاسم هاشم
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم اختبار قابلية عشرة عزلات للعفن Aspergillus fumigatus على انتاج الغليوتوكسين بوساطة تخمرات الحالة الصلبة وباستخدام الرز كوسط زرعي (ركيزة). اشارت نتائج الغربلة ان العزلة AF - 5 كانت هي الافضل واعطت اعلى انتاجية.كانت الظروف المثلى لانتاج السم على وسط الر | The ability of Ten Aspergillus fumigatus isolates for gliotoxin production were screened by solid state fermentation (SSF) using rice medium as substrate. The results indicated that, the AF - 5 isolate was the highest gliotoxin producer.The optimum conditions for gliotoxin production by AF - 5 isolate on rice medium were : - inoculum size 6×106 spores, moisturizing ratio (5 : 1) (w : v) with distilled water and incubation at 37 ?C for 10 days. Gliotoxin was purified from crude extract of AF - 5 isolate after production under the optimum conditions using solid state fermentation SSF. Purification of gliotoxin was achieved by two steps including : filtration and solid phase extraction. These processes were accessed to remove debris from crude extract and gives pure gliotoxin. The gliotoxin concentration was (122.6 ppm), which was detected by Thin layer Chromatography and High Performance Liquid Chromatography.The result of cytotoxicity effect of both purified extract and standard gliotoxin on human lymphocytes with exposure time of 24 hours at four different concentrations 25, 50, 100 and 200 ppb showed growth inhibition percentage 21, 39.10, 61.99, 87.45% and 17.89, 34.92, 58.34, and 85.22% respectively. The cytotoxicity effects have been done using conversion of the MTT to MTT formazan. After DNA was extracted from lymphocyte treated with toxin and analyzed by electrophoresis on a 1% agarose gel, gliotoxin appeared to have ability to damage the DNA. Results showed that both growth inhibition and DNA damage were increased gradually with the increasing of gliotoxin concentration.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

اسم المؤلف: مروة صباح طالب
اسم المشرف: محمد نافع علي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

اسم المؤلف: مروة قيس ابراهيم العبيدي
اسم المشرف: ماهر زكي فيصل الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

اسم المؤلف: مريم خميس عبد ربة بريس
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

اسم المؤلف: بسمة علي جاسم
اسم المشرف: مختار خميس محمد سعيد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group

الفعالية المضادة للاحياء المجهرية والانزيمية للفطريات المستنبتة المعزولة من نبات الاس Myrtle (MyrtuscommunisL.) Antimicrobial And Enzymaticactivity of Endophyticfungiisolatedfrommyrtle (Myrtuscommunisl.)

اسم المؤلف: هدى محمد كاظم ديوان
اسم المشرف: بتول زينل علي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية عزل وتشخيص الفطريات المستنبتة في اوراق نبات الاس Myrtus communis واختبار فعاليتها المضادة للاحياء المجهرية وفعاليتها الانزيمية. - اظهرت نتائج عزل الفطريات المستنبتة في 500 قطعة اوراق للحصول على عدد كلي للعزلات مساويا الى 99 عزلة ف | The present study was aimed to isolate and identify endophytic fungi from leaves of myrtle Myrtus communisand study their antimicrobial and enzymatic activities. - Results of fungal isolation from 500 leaves fragments revealed isolation of 99 endophytic fungi represented a total colonization frequency (CF) of 20.4%. These fungi were related to 23 species or isolates, where Aspergillus spp. prevailed over all others (11 species) with CF of 14% for all Aspergilli, Aspergillusniger and Aspergillusflavus showed the highest CF 7.2% and 3% respectively, other Aspergilli represented Aspergillusparvulus (CF 1.6%), Aspergilluscandidus (0.4%), Aspergillusraperi, Aspergillusclavatus, Aspergillusglaucus, Aspergillusornatus, Aspergillussclerotioniger, Aspergillusterreus and Aspergilluswentii were all showed CF of 0.2%.Other isolates fungi from leaves fragments were four morphologically different isolates of Penicillium with 0.4% CF for each. Additionaly, isolates of Cladosporium sp. (2%) and Cladosporiumcladosporoides (0.4%), as well as Cunninghamella sp., Drechsleraaustraliensis, Alternariaraphani, which showed 0.2% CF for each, Paecilomyces variotii (0.6|%), and different isolates of sterile fungi (White and Hyaline colonies) were also obtained. - Study the antagonistic activity of isolated endophytes using dual culture method against five plant pathogenic fungi(Fusarium oxysporum, Fusarium graminearum, Fusarium sp., Macrophomina phaseolinaandRhizoctonia solani)taking into account the percentage of inhibition of pathogenic fungi, results revealed variation of antagonistic activities between endophytes and pathogens. Different cases of antagonism were also appeared ranged from mycoparasitism of the endophytes on pathogens or vice versa, to competition between the two fungi and to the appearance of inhibition zone between them, this result may indicates the affinity of using some isolated endophytes in biological control applications against or controling fungal plant diseases. - Effect of ethylacetate extracts of culture broth of active endophytes shown in the last experiment against two bactria E.coli, S.aureus and the yeast Candida albicans using disc diffusion method, where filter paper discs were imprignated with different concentrations of fungal broth extracts. Results showed variation of inhibitory activity against bacteria with different endophytes, bacteria and conc. of the extract. Regarding the effect onC.albicans and filamentous fungi, most fungal broth extracts showed no inhibitory activity against yeast and no extract as well showed inhibitory activity on filamentous fungi. These results give indication thatbroth extracts contain antibacterial compounds. - Addition of 20% crude fungal broth of some endophytes to the culture medium showed significant inhibitory activity against filamentous fungi which did not respond to broth extracts, percentage of inhibition ranged between 0 - 77.08% and maximum inhibitory activity was shown by Penicillium sp. Isolate. These results give indication that some of crude filtrates contain antifungal compounds. - Analysis of broth extracts of some endophytes using TLC technique showed the content of extract of number of UV illuminated spots isolated on TLC pates having different Rf values, Bioautography showed inhibitory activity of some of these isolated spots against E.coli, S.aureus and the Filamentous fungus F.oxysporum. - Testing the extracellular enzymes activities of isolated endophyles on solid media, enzymes included amylase, lipase, pectinase, laccase, cellulase and protease showed variation in secretion of these enzymes, as well as, no endophytes showed the affinity of secreting all tested enzymes. Results also showed the high affinity of some endpohytes to secrete enzymes that could be use in multiple important applications after separation andcharacterizationof these enzymes.

التحري عن نوعية مياه الشرب لمحطتي معالجة مياه الحي والبشائر في محافظة واسط جنوبي العراق Investigation of Drinking Water Quality In Al - Haay And Al - Bashaar Water Treatment Plants In Wasit Province Southern Iraq

اسم المؤلف: وسام باسم محمد التميمي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: م اجراء فحوصات فيزيائية وكيميائية واحيائية لمياه الشرب في محطتي الحي والبشائر وعدد من المناطق التي تغذيها, شهريا للمدة من تشرين الاول 2013 الى شهر تموز 2014, عبر مراحل التصفية وصولا الى المنازل التي تقع على مسافات مختلفة عن مصدر التجهيز وبواقع نموذجين لكل | Physical, chemical and a biological tests were carried out and drinking water samples were collected from AL - Haay and AL - Bashaer water treatment plants and number of residential areas fed by these plants for period extended from October 2013 to July 2014, through the purification stages up to residential sites at different areas situated with various distances from the supplying source, two samples were taken monthly. Air temperature at sampling time was varied from 16°C to 42°C and from 10°C to 34°C, for water temperature at sampling time also. The results of pH values were within the allowable limits, ranging from 7.1in autumn to 8.1 in summer. For EC, the highest value recorded for raw water was in winter with 1338 µs/cm, while the lowest value was in spring with 920 µs/cm. The highest value recorded for drinking water was again in winter with 1330 µs/cm, and the lowest value was 910µs/cm in spring also. The highest value of turbidity recorded for raw water was in winter with 89 NTU and the lowest value was in spring with 26 NTU, while the highest mean value recorded for drinking water was 20 NTU again in winter and the lowest value was 1 NTU in spring. The study showed that the results of TDS values ware ranged from 622 mg/L in spring to 1024mg/L in winter. The highest value of residual chlorine was recorded in summer with 4.5 mg/L; the lowest value was zero mg/L in some of the farthest points of the plants. For sulfates, values were ranged from 289 mg/L in spring to 498.4 mg/L in winter. All the recorded values of chlorides ions for all water samples were within the allowable limits, its values ranged from 98mg/L in summer to156.3 mg/L in winter. For total hardness, the values were exceeding 500mg/L and allowable limits for Iraqi standard criteria. The highest value recorded for raw water was in winter with 520 mg/L and the lowest value was in summer with 351 mg/L, while the highest value recorded for drinking water was again in winter with 516mg/L and the lowest value was 337 mg/L in summer also. For calcium values ranged from 75 mg/L in summer to 135 mg/L in winter. The results of this study showed high levels of lead, cadmium and aluminum in most water samples which collected during study period compared with those that exceeded the acceptable limits provided from the quality control unit and which are considered to have adverse effects on health. For lead, values were found to vary from 0.0030 mg/l in summer to 0.16 mg/l in winter (higher acceptable limits is 0.01 mg/L). While in case of cadmium the values lied between 0.0014 mg/l in winter and 0.015 mg/l in summer (higher acceptable range is 0.0030). However, for aluminum these data were ranged from 0.0062 mg/l in raw water during spring to 0.29 mg/l in drinking water during spring also (higher acceptable range is 0.2). The current results showed an increased in the number of autotrophic bacteria, total coliform, fecal coliform, fecal streptococci, and E. coli during winter season in most study locations as compared to those of the other seasons for both water plants raw and drinking water while the FS values were less than those of other bacterial types for both water plants. The Total plat count results of drinking water were exceed 100 cell/ml, the allowable limit for drinking water, for some samples of both water plants. on the other hand, the TC, FC and E. coli exceeded zero cell/100ml, the allowable limits for drinking water, in many drinking water samples for both water plants

دراسة بعض تاثيرات اللقاح المحضر من العزلة المحلية لبكتيريا Klebsiella pneumoniae Study of Some Effects of Prepared Vaccine From Local Strain of The Klebsiella Pneumoniae

اسم المؤلف: ياسر عبد الجبار عبود السوداني
اسم المشرف: عصام فاضل علوان الجمیلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم جمع خمسين عينة سريرية من قشع مرضى مصابين بذات الرئـــة. وذلك للمدة من تشرين الثاني 2013 ولغاية ايار 2013 من مستشفى ابن البلدي ومستشفى بغداد وذلك لعزل وتشخيص بكتريا Klebsiella pneumoniae التي تعد احدى العوامل المهمة المسببة لاصابات الرئة. واخضعت عينات | Fifty clinical samples collected from sputum of patients who suffered from pneumoniae in Ibn - Balady hospital and the hospital in Baghdad city during the period from November 2012 to May 2013 for the isolation and identification of Klebsiella pneumoniae, one of the important causative agents of infection occurs in the lungs. Sputum samples were subjects to the standard laboratory procedures including identification by biochemical test and VIETK system. The results showed 15 isolates were revealed as Klebsiella Spp, only 10 isolates represented K.pneumoniae, The isolates were examined to produce extracellular toxic complex (ETC) it was found that the isolate named K2 was the higher production. Two method for purification the extracellular toxic complex (ETC) were used, first Aqueous two phase systems, In this method polymer - salt aqueous two phase system was evaluated in crude extract of K. pneumoniae at varying concentration of Dextran T - 150 with 20% with polyvinyl pyrrolidone to final rate (1 : 1) (wt : wt) with 0.2M sodium sulphate. The results showed the best concentration dilution sample given as (4.25 : 0.75) with protein concentration (97.173 mg/ml) which contained ETC in the lower layer and the mice died within 4 hours, while the second method performed by using two step column chromatography, ion exchange DEAE - Cellulose and gel filtration (Sepharose - 4B). In the first step sample given lethal activity by injection to the mice after six hours with protein concentration (55mg/ml), More purification by the second step animal died after 3hours with contain protein (27.75mg/ml). Furthermore, the results of the extracellular toxic complex characterization proved that molecular weight was 39810 Dalton determined through Gel - filtration chromatography using Sepharose 6B gel. The LD50 value of purified toxin was calculated, and the result was (6.52 mg/ml) of toxin.This quantity was found effective to cause killing of 50% of the total toxin treated animals. The biological effect of purified toxin of K. pneumoniae K2 have been examined in vivo by injection of dose (0.5 ml) of purified ETC toxin that contain (10.875 mg/ml ) protein. The final part of the study involved the histopathological changes were noted, abundant mononuclear infiltrate of inflammatory cells with necrosis of lung parenchyma. The second group of mice injected with (0.05 ml of ETC) that contain protein (1.085mg/ml) represented as sub lethal dose Histopathological changes were noted showing near of the normal appearance of alveoli and alveolar space, with presence of congestion of blood vessels. The third group of mice inject with (0.5 ml from Tris - base buffer only) represented control showed normal alveoli and alveolar space with presence of bronchial. In the immunological test the sample ETC examined with ELISA and given IgG titer (189.68+50.70 ng/ml) compared with control (46.78+12.45). This titer of IgG tested with Double immune diffusion assay and gave precipitation line with antigen compared with control.

الكشف عن الرز المحور وراثيا باستخدام انواع مختلفة من التفاعل الانزيمي المتسلسل PCR Detection of Genetically Modified Rice By Different Type of PCR

اسم المؤلف: ياسمين ابراهيم فرحان
اسم المشرف: امنة نعمة الثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In recent years, foods produced by genetic engineering technology have been on the world food markets. The biosafety aspects, regulations, and labeling for these foods are still contentious issues in most countries. Thus detection and quantificationof GMOs play crucial role for developing regulations on GM foods.In this study, eighty six non - labeled rice samples from different locals and exported market were analyzed to detect the genetic modification using a DNA based detectionvmethods as, conventional Polymerase chain reaction (PCR), and Real time PCR (RTPCR).The DNA rice samples were extracted by manual C - hexadecyl - Trimethyl - Ammonium - Bromide (CTAB) method and wizard kit method. The result revealed that DNA yield by the two methods is comparable. Rice DNA tends to be of a higher concentration when purified with the CTAB method; however, this particular DNA is more easily to amplify, the optical density (OD) was recorded 1.70 - 1.98 and the concentration of DNA quantified by fluorometer DNA rice samples, ranged from 11 to 50.5 ?g/?l. The DNA rice sample has also been used successfully with the Wizard Genomic DNA Purification Kit, and showed varieties in quality, the OD was recorded 1.65 - 1.95, and the concentration between 4.7 - 43.8 ?g/?l.The rice specific gene (sps gene) was detected by PCR. The results demonstrate that the purity of the extracted DNA in all tested rice samples was sufficiently high for a sensitive PCR analysis and the primer of detected gene appeared clearly at 251pb.Three genes; CaMV 35S promoter, NOS terminator, and insecticide resistant gene Cry1Ac were used to detect of GM rice by PCR, and Real time PCR using oligonucleotide sets targeting to novel genes. The result showed that there was no positive result reaction with conventional PCR, while the outcome of gradient PCR revealed a positive reaction in one sample (Uncle Bens brown) for CaMV35S promoter only. Gradient PCR with 12 replicons for each sample was used for qualitative detection of CaMV35S promoter gene, after optimization of melting temperature and cycles run (45 cycles) , the results appeared positive in the last three grades (63.9, 64.6, 64.9) for CaMV35S promoter, but NOS terminator, and CryIAc were recorded negative results.The result of Real - Time PCR clarified that the CaMV35S promoter specific primer showed strong amplification with Ct, and Tm values were reached into 33.73, 38.63 and 61.55, 62.92 in two samples Uncle Bens brown and Himalayan brown, respectively, whereas NOS terminator gave positive results in four samples Maxims, Laasturiana, Carolin white and Mahatma, and the values Ct and Tm reached to30.87, 30.31, 30.54, 33.75 and 64.53, 64.61, 62.62, 63.87 respectively in comparison with the positive control, while CryI Ac which did not show any positive signal.It was concluded that using molecular methods like Real - time PCR will be useful tool for detecting GM rice such as a part of the approval detection processes because of the rarity of data concerning consumption of GM rice in Iraq.

تاثير الاصابة بداء المقوسات الكوندية على المستويات الهورمونية والمدورات الخلوية خلال فترة بلوغ الانسان في محافظة بغداد The Effect of Toxoplasmosis On Hormonal And Cytokines Levels During Human Maturity In Baghdad Province

اسم المؤلف: ياسمين رياض عبد الكريم الخناق
اسم المشرف: صباح ناصر العلوجي خولة حوري زغير
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: داء المقوسات الكونديه مرض عالمي الانتشار حيث يصاب معظم الاشخاص ذوي المناعة الجيدة بطفيلي المقوسات الكونديه وغالبا دون ظهور اعراض. ان الهدف الرئيسي لهذه الدراسه هو فهم الفروقات الجنسيه, الهرمونيه والمناعيه في سن البلوغ في الاشخاص الذين لديهم اجسام مضاده نو | Toxoplasmosis is a worldwide disease where most healthy, immunocompetent individuals infected by Toxoplasma are almost asymptomatic.The primary goal of this study is to perceive the hormonal and immunological sex - differences in puberty age who have positive anti - Toxoplasma IgG specific antibodies. The secondary goal is to inspect the endocrine - immune interaction in these persons by detecting the effect of testosterone and oestradiol hormones level on cellular immune response namely, IL - 4, IL - 12. From the first of November 2012 till the end of April 2013, 303 blood samples were collected from apparently healthy male and female students of Al - Erfan, Ignadeen and Algawahery schools and Baghdad University, Both sexes where divided into two age groups : group (A) which included subjects with age range (12 - 15) years old and group (B) which included subjects with age range (16 - 19) years old. All serum samples were tested for toxoplasmosis by using Latex agglutination test and ELISA anti - Toxoplasma IgG antibodies test. As well as, all serum samples were tested by using ELISA technique for detection of serum mean concentration of testosterone, oestradiol hormones, IL - 12 and IL - 4. The results revealed that 107/ 303 (35.31%) of the studied subjects showed seropositive toxoplasmosis, 60 males and 47 females of 107 positive samples showed high significant (p<0.01) differences in comparison to uninfected subjects. Males group B have recorded the highest percentage 34(41.46%) of the infection. Positive association was found between toxoplasmosis and testosterone level in asymptomatic toxoplasmosis cases compared to uninfected group. high mean concentration of testosterone in toxoplasmosis infected males recorded (15.03± 1.04 ng/ml) and (12.4± 0.91 ng/ml) in groups A and B respectively, in comparison to control group which recorded (8.03± 0.78) and (9.86± 0.83) in groups A and B respectively. Also toxoplasmosis infected females revealed high levels of testosterone hormone which represented (4.83± 0.06 ng/ml) and (2.55± 0.03 ng/ml) in groups A and B respectively, with a significant (p?0.05) differences between them, while the control group recorded (0.10± 0.02 ng/ml) and (0.90±0.03 ng/ml) in group A and B respectively.The present study showed a significant (P?0.05) decrease in the mean concentration of oestradiol E2 hormone in toxoplasmosis infected males and females in comparison with uninfected ones. E2 mean concentration was (41± 2.48 ng/ml) and (56± 2.91 ng/ml) for male groups A and B, respectively, in comparison to control group which recorded (67± 2.08 ng/ml) and (74± 2.42 ng/ml) in group A and B respectively, while it was (188 ± 12.48 ng/ml) and (196 ± 16.52 ng/ml) for female group A and B respectively, in comparison to control group which recorded (221±12.09 ng/ml) and (233± 15.63 ng/ml) for group A and B, respectively.The mean concentration of E2 hormone in toxoplasmosis infected females according to their menstrual cycle showed low levels in ovulation, late follicular and luteal phases, which represent (37.5 ± 2.59 ng/ml), (131 ± 16.7 ng/ml) and (76± 3.92 ng/ml) respectively, while the mean concentration of this hormone in uninfected females was (52.4± 2.88 ng/ml), (271.6± 21.04 ng/ml) and (196.2± 12.37 ng/ml) in the three phases of menstrual cycle. This study showed high significant (p?0.05) level of IL - 12 in both males and females with latent toxoplasmosis in comparison with free - toxoplasmosis groups. The mean concentration of IL - 12 in infected males was (4.75 ± 0.88 pg/ml) and (4.12 ± 0.69 pg/ml) in male groups A and B respectively, in comparison to control group which was (2.86± 0.53 pg/ml) and (2.46± 0.62 pg/ml) in groups A and B respectively, while it was (5.60 ± 0.12 pg/ml) and (6.04 ± 0.26 pg/ml) in infected female groups A and B respectively, in comparison to control group which recorded (3.32± 0.89 pg/ml) and (4.27± 0.15 pg/ml) in group A and B respectively. IL - 4 recorded quite elevated level in toxoplasmosis infected males (groups A and B) which was (15.09 ± 0.92 pg/ml) and (17.67 ± 0.78 pg/ml) respectively, in comparison to control group which recorded (13.89± 0.84 pg/ml) and (14.92± 0.69 pg/ml) in groups A and B respectively, Meanwhile the mean concentration of IL - 4 in toxoplasmosis infected females showed mild elevation in both groups A and B which was (15.14 ± 0.84 pg/ml) and (16.06 ± 1.13 pg/ml) respectively, in comparison with toxoplasmosis free subjects which recorded (16.53± 1.22 pg/ml) and (15.15 ± 0.97 pg/ml) with no significant differences between them. Interactions between the endocrine and immune systems may mediate sex differences in response to toxoplasmosis infection.

التحري عن التعبير الجيني لل FOXP3 وTGF - ?1 باستخدام الطرائق الجزيئية والمناعية في سرطان الرئة اللاصغير الخلية Detection of FOXP3 Gene Expression And TGF - ?1 Using Molecular And Immunological Methods In Non - Small Cell Lung Carcinoma

اسم المؤلف: سهاد فيصل حاتم المقدادي
اسم المشرف: امنة نصيف جاسم بان عباس عبد المجيد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Studies have linked FOXP3 and TGF - ? expression to the outcome of certain cancers. FOXP3 is a marker known to be expression in T - regulatory cells while TGF - ? is a secreted protein usually detected in the extra cellular matrix.The present study aimed at focusing on the identification of immune markers namely FOXP3 and TGF - ? with their expression patterns in lung cancer patients as a useful tool to predict disease progression.Also it is aimed to design molecular evaluation of m RNA expression of both FOXP3 and TGF - ? in peripheral blood mononuclear cells and bronchial (brush) cells of patients with lung cancer and benign lesions, using qRT PCR; determining the T - reg level in the peripheral blood employing the High Rsolution Melting (HRM) as a novel method to detect Treg - specific demethylated region (TSDR); molecular DNA analysis of somatic mutation of exons 3, 6, 7 of FOXP3 in patients with lung cancer tissue and benign lesions and immunohistochemical (IHC) estimation of FOXP3 and TGF - ?1 in T - reg and cancer cells in formalin fixed paraffin embedded(FFPE)lung cancer tissue and benign lesions.Blood samples were collected from 30 patients with newly diagnosed, non small cell lung carcinoma and 30 patients with benign lesions.Patients were recruited at The Specialized Surgery Hospital and Oncology Teaching Hospital/Baghdad.Samples from 16 apparently healthy donors were used as control during the period from June 2012 to June 2013. The samples preservation with TRIzol reagent were subjected to molecular study including RNA and DNA extraction; reverse transcription; RT - PCR; HRM assay and DNA sequencing were done in the Molecular Oncology Unit/Guys and ST Thomas? s hospital/ King College/London/UK.The expression level of FOXP3 was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of FOXP3 expression(fold change)was significantly high(2.64±0.09)in cancer cases than in benign cases(1.32±0.04)and healthy control(1.38±0.06)with p<0.05.A significant association between high expression level and >60 age and squamous cell carcinoma in cancer cases P<0.05.The expression level of TGF - ? was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of TGF - ? expression (fold change) was significantly high (6.27±0.56) in cancer cases more than healthy control (2.87±0.09) with p<0.05.The association was significant between TGF - ? expression level (high and low) and age>60in cancer and benign groups (p<0.05), while no significant association with gender and cancer types were noticed.For FOXP3 mRNA expression in bronchial brush cells, the result showed no significant difference between the mean fold change of malignant(3.57 ± 0.06) and benign(4.02 ± 0.06) patients. The low expression was predominated both in cancer and benign cases. No significant differences were found between FOXP3 expression (high and low) and age; gender ; cancer types..According to FOXP3 T - reg specific demethylated region detection, results showed that the mean percentage of FOXP3demethylation in lung cancer patients (4.32 ± 0.04) was significantly higher than in benign lesions (3.22 ± 0.02) patients andhealthy controls(3.33±0.04). A positive correlation coefficient with high significant, was found in the group of cancer samples (R² = 0.6653;r = 0.69;P : 0.0017)on correlating percentage of Treg and demethylation of FOXP3 from one side with its m RNA expression on the other side.In benign lesion group was(R² = 0.5334;r= 0.59;p= 0.0027), While in the control group a positive correlation but a weak significance was found (R²= 0.2383;r=0.28;P=0.0437).FOXP3 gene sequencing revealed high frequency of missense mutations c.715 GTA>CTA : V 239 L in 17 (94.44%) in malignant sample and non cancerous cases7 (87.5%)without statistical differences. Missence mutations were also detected in exone 3 in 3(16.67%) cancer cases and in 1(12.5 %) benign lesion.No missense mutations could be detected in exon 6. Intronic mutations and silent mutation were variable in three exons without statistical differences. Many cases of adenocarcinoma have shown multiple mutations either of missense or Intronic types. Missense mutations of exon 7 were correlated significantly with an age of 60?years. Exon 3 mutations were significantly associated with adenocarcinoma. Positive FOXP3 Immunohistochemistry (IHC) staining in tumor cells was associated with high missense mutations frequency 10(55.55%) in exon7, while in exone 3 was 2(11.11%). Negative FOXP3 IHC staining in the tumor cells was associated with seven missense mutations in exon7 and one (5.55%) in exon 3, in addition 4(23.53%) cases of the exon 7 missense mutations were associated with negative FOXP3 expression in lymphocytes.The result showed that FOXP3 by using IHC staining was positive in 21(70%) of nuclei of cancer cells, and 22(73.3%)in Treg infiltrates.The positive cancer cells and Treg infiltrates associated significantly with age>60 (p<0.05).No significant association was found withgender, cancer type, while there is association with moderate differentiation compared to poorly differentiation (p<0.05). High frequence of FOXP3 expression score 3 and high intensity were appeared in nuclear cancer cells compared to benign lesions cells, while Treg infiltrates with score 1 and high intensity was high frequency in malignant and benign.The result showed that TGF - ?1 by using IHC staining was positive in 25(83.3%) in the cancer cells and 21(70%) in stromal cells. No significant difference was noted between positive expression in malignant and benign lesions p>0.05.No significant association was noticed between positive cells expression and age, gender, cancer type and differentiation p>0.05. High frequency of TGF - ?1 expression score 3 and high intensity in malignant cells and benign. Also stromal cell expression score 3 and high intensity were predominated in malignant and benign lesions. The high and moderate intensity expression was more frequent in matrix surrounding cancer cells compared to non cancerous.Total agreement and kappa coefficient between FOXP3 and TGF - ?1were poor in malignant and benign epithelial cells and stroma, while the perfect agreement was between expression of TGF - ?1in stromal cells

تعدد الاشكال الوراثي للحركيات الخلوية وHLA - DQB1 في مرضى السل الرئوي Genetic Polymorphisms of Cytokines And HLA - DQB1 In Pulmonary Tuberculosis Patients

اسم المؤلف: خلود كريم حسن
اسم المشرف: علي حسين ادحية
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aimed to understand the correlation between serum level of nine cytokines (IL - 1?, IL - 1RA, IL - 2, IL - 4, IL - 6, IL - 10, IL - 12, IFN - ? and TNF - ?) and their genetic polymorphisms at 16 gene positions defined by sequence specific primer - polymerase chain reaction (SSP - PCR) in pulmonary tuberculosis (PTB) patients, and in addition HLA - DQB1 gene polymorphism was also defined by SSP - PCR to determine their role in susceptibility or resistance to M. tuberculosis. Finally, serum level of cortisol was also determined in the patients.Ninety four Iraqi Arabs PTB patients (70 males and 24 females) were enrolled in the study. They were referred to the Institute of Chest and Respiratory Diseases in Baghdad for diagnosis and treatment during the period May - October 2012. A control sample of 80 apparently healthy individuals was also included and matched patients for gender (60 males and 20 females) and ethnicity. The results are summarized in the following : 1. A significant increased serum level of IL - 1? (24.16 ± 8.82 vs. 3.20 ± 1.18 pg/ml), IL - 1RA (41.31 ± 6.64 vs. 16.85 ± 5.50 pg/ml), IL - 2 (17.63 ± 3.53 vs. 7.80 ± 1.10 pg/ml), IL - 4 (9.56 ± 2.60 vs. 3.81 ± 1.70 pg/ml), IL - 10 (34.49 ± 4.60 vs. 7.61 ± 1.70 pg/ml), IL - 12 (25.16 ± 5.85 vs. 7.70 ± 1.12 pg/ml) and TNF - ? (22.52 ± 4.41 vs. 4.97 ± 1.15 pg/ml) was recorded in PTB patients compared to controls. Also, Cortisol serum level was significantly increased in patients (215.47 ± 1.33 vs. 38.63 ± 1.74 ng/ml).2. Cytokine gene polymorphism analysis revealed that neither genotypes nor alleles of IL1A - 889, IL2 - 330, IL2+166, IL4 - 590, IL4 - 33, IL6+565, IL10 - 819, IL10 - 592, IL12B - 1188 and TNF - 238 genes showed a significant variation between PTB patients and controls. In contrast, the frequency of TT genotype of IL1RN gene at position mspal 11100 showed a significant (P = 0.004) increase in PTB patients compared to controls (65.9 vs. 43.7%). For IL4 - 1098, the frequency of TT genotype was also significantly (P = 0.048) increased inpatients (82.9 vs. 70.0%). At position - 174 of IL6 gene, a significant (P = 0.002) increased frequency of GG genotype was observed in patients (55.3 vs. 31.2%). For IL10 gene, only GG genotype at position IL10 - 1082 was observed with a significant (P = 0.045) increased frequency in patients (18.1 vs. 7.5%). At position - 308 of TNF gene, a significant (P = 6.9 x 10 - 5) decreased frequency of GG genotype was observed in patients (60.6 vs. 87.5%), while GA genotype was significantly (P = 1.3 x 10 - 4) increased (38.2 vs. 12.5%). Finally, the frequency of AA genotype of IFNG gene at position +874 demonstrated a significant (P = 0.006) increase in PTB patients (55.3%) compared to controls (33.7%).3. To determine the impact of cytokine genotypes on cytokines serum level, PTB patients and controls were distributed according to their serum level in the three genotypes of each cytokine. It was found that CC genotype of IL1RNmspal 11100 in patients was observed with the highest IL - 1RA level (52.16 ± 5.81 pg/ml) compared to TT (41.39 ± 3.23 pg/ml) or TC (38.10 ± 4.54 pg/ml) genotype. The TT genotype of IL2 at position - 330 also showed the highest level of IL - 2 (22.16 ± 4.31 pg/ml) compared to TG (17.59 ± 3.40 pg/ml) or GG (13.68 ± 3.53 pg/ml) genotype in patients. The IL4 - 1098 TT genotype showed the highest level of IL - 4 in patients (10.38 ± 2.21 pg/ml) compared to TG (6.09 ± 1.20 pg/ml) or GG (3.93 ± 0.80 pg/ml) genotype. For IL10 gene, the GG genotype of IL10 - 1082 recorded the highest level of IL - 10 (40.67 ± 2.96 pg/ml), which was significantly different from AA genotype (26.66 ± 5.65 pg/ml). At position - 308 of TNF gene, serum level of TNF - ? in GG genotype of patients demonstrated a significant increased mean compared to genotype GA (24.76 ± 1.30 vs. 19.15 ± 1.12 pg/ml). At position - 238, TNF GG genotype showed a significant increase level of TNF - ? (23.02 ± 2.91 pg/ml) in patients compared to AA genotype (17.18 ± 1.53 pg/ml) of patients. Finally, IFNG+874 AA genotype was observed with the highest IFN - ? level in patients (11.07 ± 1.12 pg/ml) compared to AT (7.97 ± 1.81 pg/ml)or TT (6.10 ± 2.20 pg/ml) genotype. In contrast, no such differences were observed in controls.4. Out of the five encountered HLA - DQB1 alleles, DQB1*03 showed a significant (P = 0.005) increased frequency in PTB patients compared to controls (71.3 vs. 50.0%). It was also observed that heterozygosity at such gene locus was significantly (P = 0.03) more frequent in patients than in controls (93.6 vs. 82.5%), while homozygosity was observed with a less percentage frequency in patients compared to controls (6.4 vs. 17.5%) and the difference was also significant (P = 0.03).Accordingly, it is possible to conclude that the cytokine profile was deviated in PTB patients, and such deviation was correlated with the genotypes of some cytokines, which might also together with HLA - DQB1polymorphism confer the individual an immunogenetic predisposition to develop M. tuberculosis infection.

تقييم اختبارات PCR وطرق الزرع الاعتيادية في التشخيص المبكر لتجرثم الدم لدى الاطفال في مستشفى حماية الاطفال التعليمي في مدينة الطب / بغداد Evaluation of PCR And Culture Methods For The Early Diagnosis of Bacteremia In Children From Welfare Teaching Hospital In Medicine City /Baghdad

اسم المؤلف: زينب صالح هادي الزبيدي
اسم المشرف: محمد ابراهيم نادر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية تقييم الفحص المعتمد على تقنية PCR (polymerase chain reaction) (وطرق الزرع الاعتيادية في التشخيص المبكر لتسمم او تجرثم الدم في الاطفال.531 نماذج دم تم جمعها من الاطفال المرضى الذين اعمارهم اقل من 51 سنة ومشتبه بان لديهم اعراض تجرثم | The present study has been undertaken to evaluate polymerase chain reaction (PCR) technique in the diagnosis of bacteremia in comparison with the conventional blood culture techniques in children (infant and newborn).Blood specimens were collected from 135 children under 12 years of age suspected with fever and sepsis, obtained from Welfare Teaching Hospital/Medical City/Baghdad, for the period from April/ 2013 till January/ 2014.Blood specimens were collected and processed for Blood culture and PCR. Blood culture was performed using blood culture bottles contain brain heart infusion broth and positive results were subcultured using three media (macConkey - , chocolate - and blood agar), Gram stain, biochemical tests and conformational test (Api staph and Api 20E). Polymerase chain reaction was done using the universal primer, gram positive specific primer, gram negative specific primer, 16s rRNA primer for coagulase negative staphylococci and LacZ primer for Enterobacteriaceae.Optimization trials was carried out to increase the sensitivity of the PCR by applying 57°C in the annealing step for Gram positive specific primer and Gram negative specific primer to detect Gram positive and negative bacteria in blood respectively.Blood specimens were positive for bacteria in 69 cases (51.1%) by blood culture and 74 cases (54.8%) by PCR out of a total of 135 specimens analyzed. PCR showed more sensitive results compared to blood culture for detection of neonatal bacteremia. current results were revealed the ability of PCR to recognize five pathogens which have been negative by culture, all have been coagulase negative Staphylococci.The most frequent bacteria isolated and detected by PCR and Blood culture methods were Coagulase negative staphylococci (CoNS) (n = 60) followed by Enterobacter spp. (n = 8), E.coli (n = 5) and K.pneumoniae (n= 1). Interestingly, higher incidence rate (81.1%) were documented for the late onset sepsis (LOS) in our study compared to the early onset sepsis (EOS) (18.9%) for all bacteria. LacZ PCR efficiency have been 100% for detection of Enterobacteriaceae in blood.

تاثير انزيم الكلوكوسيل ترانسفيريز المنقى من العزلة المحلية Streptococcus mutans النمط C في انتاج الاضداد (IgY) من صفار بيض طيور الدجاج The Effect of Glucosyltransferase Purified From Local Isolate Streptococcus Mutans (Serotype C) On Egg Yolk Antibodies (IgY) Generation In Layer Hens

اسم المؤلف: هاشم محمد زهراو الصبيحاوي
اسم المشرف: عصام فاضل علوان الجمیلي فارس عبد الكريم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية عزل وتشخيص بكتيريا Streptococcus mutans المسؤولة عن تنخر الاسنان البشري والتكلسات (plaque) واستخراج اضداد لها من صفار بيض الدجاج Yolk Immunoglobullin (IgY) لغرض استخدامها مستقبلا كمثبطات لنمو هذه البكتيريا الخطيرة ويمكن مزجها مع مع | The presented study aimed to isolate the main agent for dental caries and teeth plaque, Streptococcus mutans bacteria, and then production of specific antibodies against these harmful bacteria by the use of chicken egg yolk immunoglobulin (IgY). S. mutans had been proposed as the main etiological agents of dental caries and high levels of mutans streptococci in the plaque is correlated with a higher risk for dental caries. Seventy five plaque samples were collected from human teeth. Forty two samples were considered to be positive bacterial isolates using MS - agar (Mitist Salivares agar). Thirty five isolates were considered belonging to the group Streptococci; among these isolates 29 isolates were expected to be belonging to mutans streptococci group according to ability of producing special kind of exopolysaccharides. Ten isolates were considered as S. mutans with a percentage of 41% depending on staining with triphenyltetrazolium chloride and tolerance with NaCl 4%, 6 isolates were classified as serotype C by using Lancefield grouping identification. These isolates were tested for production of extracellular Glucosyltransferase (GTF) through determination of their enzyme specific activity. All isolates were able to produce the enzyme; Streptococci isolate (H5) identified as Streptococcus mutans serotype C was selected as the best producible isolate for GTF with a specific activity of 2.6 U/mg. It was found that GTF of the chosen isolate (H5) was produced during the middle stationary phase (18 - 35 hr.) and its maximal productivity was reached at 22 hr. Purification of S. mutans serotype (C) H5 GTF were done by ammonium sulfate, ion - exchange chromatography (DEAE - Sephacel column), and gel - filtration chromatography using Sepharose 6B column. The best percent saturation use for precipitating GTF by ammonium sulfate was 20 - 40% with specific activity 3.4 U/mg. Two purified GTF enzymes (GTF - I and GTF - II) were detected with specific activity 8.3 U/mg, 35.5 U/mg after 22.6, and 96.1 fold of purification respectively with yield 17.2%. Purification S. mutans CA - GTF (H5) were done by 8M urea, ammonium sulfate, DEAE - Sephacel column and gel - filtration (sepharose 6B) column chromatography. The purified CA - GTF was detected with specific activity 18.1 U/mg after 24.5 fold of purification with yield 20.2%. Determination of purified GTF (GTF - I, GTF - II) and CA - GTF molecular weight was done by using gel - filtration chromatography (sepharose 6B) column with presence of standards proteins. It was found that the molecular weight of GTF - I, GTF - II and CA - GTF was 125.819, 112.201 and 84.139 dalton, respectively. The ability of GTF, CA - GTF and whole cell of S. mutans to stimulate the immune system of avian hens was tested. The intramuscular rout injection of three purified antigens (GTF, CA - GTF and whole cell) in the chest of experimental hens was done. IgG from egg yolk hens (IgY) was purified through the post immunization period (9 weeks) by using polyethylene glycol (PEG) precipitation and protein content of IgY antibodies was estimated from egg yolk and serum. Each one milliliter of purified IgY egg yolk samples GTF, CA - GTF and Whole cell, protein contained 7.06, 6.97, 3.9 mg/ml, respectively while in serum protein content about2.6, 3.1 and 3.25 mg/ml, respectively. The Sodium dodecyle sulfate polyacrylamide gel electrophoresis (SDS - PAGE) of anti - GTF (IgY) indicated that purified IgY gave two bands; 47.863 and 34.673dalton which were considered to be IgY heavy and light chains respectively. the IgY - CA - GTF sample is the best in terms IgY specificity 34.07% while the two samples (GTF, Whole cell) performed 30.5% and 29.3% respectively, Igy - GTF the best in terms purity 49% followed IgY - CA - GTF 47% and IgY - whole cell 46.3%. The immunological specificity of the three IgY samples preparations was assessed by ELISA test and the best sample that produced high titration was IgY - GTF with concentration 3.5 mg/ml, followed by the IgY - CA - GTF and IgY - whole cell with concentration 3.28 and 3.1 mg/ml respectively. The IgY - GTF inhibited approximate 75% of the specific activity GTF, while IgY - CA - GTF inhibited 50% of the specific activity CA - GTF. A double immunodiffusion test for detection of the immune response between anti - GTF IgY and purified GTF, CA - GTF and Whole cell antigens were recorded. The immunological response of anti - GTF and anti - CA - GTF was indicated by the appearance of precipitation lines on the surface gel between anti - GTF and two antigens GTF and CA - GTF while in the anti - CA - GTF and anti whole cell only with homologues antigen. The effect of different concentration of inhibitor (Amoxicillin) and anti - GTF, anti - CA - GTF and anti - whole cell on the growth of S. mutans bacteria were tested using broth dilution method and diffusion method on solid medium. Anti - GTF and anti - CA - GTF had no effect on the growth of S. mutans(H5) serotype C, while anti - whole - cell and Amoxicillin were capable to inhibit the growth of bacteria at concentration 20µg/ml and 15µg/ml respectively. The minimal inhibitory concentrations in which these concentrations were noticed at 35µg/ml and 30 µg/ml respectively. The highest zone of inhibition (40 mm) was recognized with Amoxicillin at concentration of 50 µg/ml, followed by anti - whole cell with a zone of inhibition of 34 mm at concentration of 70 µg/ml.

التشخيص الجزيئي لبكتريا Streptococci الفمويه باستخدام جينات gtfs في بعض مرضى السكري المصابين بتسوس الاسنان Molecular Identification of Oral Streptococci Using Gtfs Genes In Some Iraqi Diabetic Dental Caries Patients

اسم المؤلف: هالة كمال محسن القزاز
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذة الدراسة في معهد الهندسة الوراثية والتقنيات الاحيائية في جامعة بغداد خلال الفترة من تشرين الثاني 2012 الى ايار 2013، للكشف عن علاقة تسوس الاسنان بين مرضى السكري والمرضى غير المصابين بالسكري DDCP) و(NDCP اعتمادا على تسوس الاسنان من انواع Streptoco | The present study was carried out in Genetic Engineering and biotechnology Institute / University of Baghdad during the period from November, 2012 to May, 2013 to detect the relationship between diabetic and non - diabetic patients according to the dental caries occurrence and its causes by Streptococcus spp. (S. mutans, S. salivarius and S. oralis (which are isolated from oral cavity, In addition, this study was carried out to study the comparison between the traditional (bacterial culturing) and molecular diagnosis methods. The total number of the studied groups was 95 Iraqi patients (45 diabetic dental caries patients (DDCP) and 50 non - diabetic dental caries patients (NDCP) of both genders who their ages ranged from 18 - 65 years old. The patients, samples including saliva and buccal swabs that randomly collected from DDCP and NDCP who were reviewing Al - Alweyia Centers of Dental Caries and Diabetic Diseases in Al - Yarmook hospital in Baghdad city. The distribution of patients who have dental caries according to genders showed high significant differences at (p<0.01) between two genders (30 females, 15 males) for diabetic dental caries patients, while in non diabetic dental caries patients, there were significant differences at (p<0.05) between two genders (29 females, 21 males). The distribution of diabetic dental caries patients according to age showed high significant differences at (p<0.01) between two genders in age group of 36 - 50 years old, While low significant differences at (p<0.05) between two genders in age group of 20 - 35 years old and no significant differences between two genders in age group more than 50 years old, In another hand in non diabetic dental caries patients, there were no significant differences between two genders in all age groups. The results of samples (saliva and buccal swabs) culturing on mitis salivarius bacitracin agar media (MSBA) appeared that out of 95 bacterial cultures, 67 bacterial cultures were grown (32 bacterial cultures for diabetic dental caries patients and 35 bacterial cultures for non diabetic dental caries patients); S. mutans, S. salivarius, and S. oralis species were identified according to the results of microscopic examination, API kit 20 - strep, hemolysis on blood agar, motility test and catalase test. The molecular study focused on the analysis of DNA which extracted directly from saliva, buccal swabs and from the bacterial culture cells of S. mutans, S. salivares and S. oralis from both diabetic dental caries patients and non diabetic dental caries patients. Polymerase chain reaction (PCR) results revealed the presence of the product with 433, 544, and 374 bp which were related to gtfD (S. mutans), gtfK (S. salivarius) and gtfR (S. oralis) respectively in all samples (saliva, buccal swabs and bacterial culture). According to the presence of these three genes, there were high significant differences at (p<0.01) between diabetic dental caries patients and non diabetic dental caries patients, while there were no significant differences according to the percentage of presence of each gene between the three species of bacteria. Sequencing of the PCR products of the gtfs (gtfD, gtfK, and gtfR) genes region showed that nine samples gave acceptable results according to National center for Biotechnology Information (NCBI) matching, while 3 samples gave no results; this may be due to an error in sequencing system. The sequencing analysis of gtfs gene (gtfD, gtfK and gtfR) revealed that in DDCP the highest percentage of recorded mutations was in the gtfR gene.While in non diabetic dental caries patients, the highest percentage of recorded mutations in the gtfK gene than gtfR genes. In gtfR gene all mutations were substitution for diabetic dental caries patients and non diabetic dental caries patients. Nevertheless, in diabetic dental caries patients the mutations in gtfK and gtfD genes distributed between substitution and deletion mutations without recording any type of insertion mutation. But, in non diabetic dental caries patients, in gtfD all mutations were distributed between three types of mutations (substitution, insertion and deletion). The highest percentage of the effect of mutations in gtfs genes (gtfD, gtfR and gtfK) in diabetic dental caries patients were silent and missense mutation's than the frameshift mutations. on the other hand, the highest percentage of the effect of mutations in gtfs genes (gtfD, gtfR and gtfK) in non diabetic dental caries patients was missense mutations as compared with the other two types of silent and frameshift mutations

دراسة تاثير ضوء الليزر الثنائي الصمام (632 نانومتر) على بكتريا المكورات العنقودية الذهبية بوجـود المثلين الازرق كمتحسس ضوئي Study of Photodynamic Effect of (632 nm) Laser Diode Light On Staphylococcus Aureus Using Methylene Blue As A Photosensitizer

اسم المؤلف: ضياء خليل اسماعيل
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شملت الدراسة ثمان وسبعين عزلة من بكتريا المكورات العنقودية الذهبية عزلت من مئة وعشرين عينة تم الحصول عليها من عينات حروق وجروح قيحية وتجرثم الدم ومن مرضى يعانون من التهابات المجاري البولية, ومن اكثر من مستشفى للفترة من تشرين الثاني‏ / لغاية اذار/ 2014.تم | Seventy eight isolates of S. aureus bacteria were obtained from one hundred and twenty samples which were collected from different body sites and lesions (urine, blood and purulent wounds and burns) of patients from both sexes during the period November - 2013 to March - 2014. Methicilline sensitivity test (5µg MET disc) showed the appearance of methicillin - resistant in thirty seven Staphylococcus aureus isolates. Following exposure to laser light with a wavelength of 632 nanometer in the presence of Methylene blue at a concentration of 300µM at various exposure times (2, 4, 6, 8, 10, 12 and 15min), the results showed that the maximum decrease in viable colony counts ranging approximately from (6.9 to 3.8) log10 CFU /ml. Highly significant reduction in the viable count was achieved at 10, 12, 15 min exposure times, and 99% killing of cells were obtained when Photosensitisation of S. aureus using diode laser light at an energy density at 458.6 watt/cm2 for 15 mints. While in their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the S. aureus isolates. Both of phenotypic and genotypic investigation of the changes in virulence factors and the antibiotic - resistance were evaluated before and after irradiation with laser light.Results of photosensitization susceptibility tests showed large variations in the susceptibility, the isolates with resistant to methicillin before laser irradiation, become sensitive to it with percentage of 21.6%; in contrast the isolates with sensitive to vancomycine become resistant to it with percentage of 32.43%.On the other hand, the isolates that were resistant to Cefotaxime before laser irradiation become within the sensitivity range after laser irradiation with percentage of 51.35%, and also there were isolates within the sensitivity range before laser, become sensitive to Ciprofloxacin with percentage of 27.02% after irradiation. And the isolates of the S. aureus with resistant to Norfloxacin before laser irradiation, become sensitive to it with percentage of 16.2%. Also resulted in decrease the activity of ? - haemolysis, with 33 (90.3%) isolates of S. aureus in comparison to control as shown in blood agar method assay. In contrast had no effect on thermonuclease enzyme after irradiation.Detection of three genes represented in MRSA isolates by a confirmatory test was carried out using Polymerase chain reaction (PCR) technique. The results of the PCR amplification of mecA gene noted that it was present in 27 (72.2%) S. aureus isolates, While hlb gene detected 17(46%) isolates of 37 samples of S. aureus isolates, 14 of 17 hlb - positive S. aureus isolates(82.3%) were showed reduction in toxin production after exposure to laser light, whereas no altered or deficiency in thermonuclease gene (nuc).

علاقه بعض طرز الجين كالبين - 10 مع حدوث مرض السكري من النوع الثاني في العراق Association of Some Calpain - 10 Gene Polymorphisms With The Incidence of Type 2 Diabetes Mellitus In Iraq

اسم المؤلف: مياسة مثنى خالد
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسه الحاليه في معهد الهندسه الوراثيه والتقنيات الاحيائيه - جامعة بغداد خلال الفتره من كانون الثاني ولغاية حزيران - 2013 للكشف عن علاقة بعض الطرز الجين كالبين - 10 بحدوث مرض السكري من النوع الثاني في العراق. تم استخلاص الدنا من الدم الكلي باستخد | The present study was carried out in Genetic Engineering and biotechnology Institute - Baghdad University during a period from January to June, 2013, for detecting the association of some calpain - 10 gene polymorphisms with the incidence of type 2 diabetes mellitus in Iraq. Genomic DNA was isolated by using Geneaid DNA extraction kit from the whole blood; conventional PCR (SNP - 44 and Del/Ins - 19) and PCR - RFLP (SNP - 43 and SNP - 63) were used to detect the calpain10 variants by using specific primers and restriction enzymes. The study population consisted of 50 subjects with type 2 diabetes and 50 with normal fasting blood glucose (80 - 110 mg/dl). The type 2 diabetic subjects were recruited from the National Center for Diabetes treatment and Research. The non - diabetic control subjects were recruited from the same area as the comprising blood donors, healthy volunteers, or hospital /university staff members. Previous studies have detected a role for Calpain - 10 (CAPN10) polymorphisms in susceptibility to Type 2 diabetes mellitus (T2DM) in many populations. This study aimed to evaluate possible associations between these polymorphisms in the CAPN10 gene (SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63) and T2DM incidence in Iraqi population. Enrichment of allele 1(2R) in Del/Ins - 19 and 2R/2R genotype were found in T2DM patients. While the alleles and genotypes distribution of SNP - 44, SNP - 43 and SNP - 63 were not significantly different between patient groups and non - diabetic control subjects. The genotype AA in SNP - 43 and genotype TT in SNP - 63 were not found neither in T2DM nor in control subjects. of the eight haplotypes detected, enrichment of both haplotype 112 defined by variants of SNP - 43, Del/Ins - 19, and SNP - 63 and haplotype 2112 defined by variants of SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63 were seen in patients. The distribution of the other haplotypes was comparable between patients and control subjects. The calpain10 haplotype combinations were also obtained, and the haplotype combinations 111/111 and 111/112; which are created by variants of SNP - 43, del/ins - 19 and SNP - 63 and; haplotype combinations 1111/2111, 1111/2112 and 1121 / 2222; created by SNP - 44, SNP - 43, del/ins - 19 and SNP - 63; were associated with increasing the risk of T2DM.

التاثيرات المضادة للاكسدة والسمية الخلوية لمركب اللكنان المنقى من بذور نبات جوزة الطيب Antioxidant And Cytotoxic Effects of Lignan Purified From Myristica Fragrans Seeds

اسم المؤلف: شیماء عصام عبد الوهاب البرزنجي
اسم المشرف: عصام فاضل علوان الجمیلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In this study, natural lignan dimer was isolated from nutmeg seeds (Myristica fragrans) using organic solvent, partially purified using liquid/liquid partiation, purified using anion exchanger and chemically characterized using Benedict’s Reagent, Fehling’s Reagent and Molish’s Reagent. Then, by the aid of UPLC - PDA - IT - TOF - MS System, the molecular weight (626.221 Dalton) and the molecular formula (C39H45O7) of this dimer were determined. After that, the free radical scavenging activities were studied using stable free radical compound 1, 1 - Diphenyl - 2 - Picryl - hydrazil (DPPH). Results showed that 100, 10, 1 and 0.1 ?g/ml of purified lignan had 76.7 %, 65%, 28% and 8% scavenging activity respectively, while the same concentrations of partial purified lignan had 44.3%, 18.5%, 11% and 0% scavenging activity respectively.MTT(3 - (dimethylthiazol - 2 - yl) - 2, 5 - diphenyl tetrazolium bromide) assay was conducted to determined the IC50 for both purified and partial purified lignan using 4 different cell lines A549 (human lung adenocarcinoma epithelial cells), MCF7 (breast cancer cells), PC3(human prostate cancer cells), and HepG2(liver hepatocellular cancer cells), and to determine which cells type were be affected more by this natural lignan dimmer.The IC50 values for purified lignan were 85.17, 51.16, 108.4 and 60.21 ?g/ml while the IC50 values for partial purified one were 170.1, 84.14, 154.4 and 151.3 ?g/ml using A549, MCF - 7, PC - 3 and HePG2, respectively.The high content screening analysis (HCSA) and Cellomics Thermo Scientific maltiparametric Kits were used for the evaluation of cell - lignan interaction; 100, 50 and 25 ?g/ml of purified lignan caused 87.22, 69 and 53.36% reduction in MCF - 7cell count respectively and the same concentrations caused 98.1, 97.5 and 98.55% nuclear morphology changes. Results also revealed that these concentrations caused 7.7, 7.0 and 5.83% increase in MCF - 7 cells permeability respectively and they also caused 12.22, 11.15 and 0.2%decrease in mitochondrial membrane potential respectively, while these concentrations caused 11.12, 10.1 and 10% increase in Cytochrome C releasing from mitochondria to cytoplasim respectively.Reactive oxygen species (ROS) induction in MCF - 7 cells in the presence of 200, 100 and 50 ?g/ml of purified lignan caused 20, 11.48 and 9.61% ROS reduction respectively.MCF - 7 cell cycle was studied in the presence of 100, 50 and 25 ?g/ml of purified lignan, and results revealed that this compound blocked cell cycle at Sphase and the percentages of S - phase cells reduction were 74.33, 52.4 and 67%, respectively. This reduction was dose dependent while the same concentrations had no effect on MCF - 7 mitotic cells. Cell cycle arrest was detected immunofluorescently using BrdU antibodies (S - phase cell staining) and phosphor - Histone H3 antibodies (M - phase cells staining

التشخيس المبكر لداء السكري النوع الاول باستخدام مضاد حمض الكلوتاميك منزوع الكاربوكسيل ومضاد البيروكسيديز الدرقي Early Detection of Type 1 Diabetes Mellitus Using Anti - Glutamic Acid Decarboxylase And Anti - Thyroid Peroxidase

اسم المؤلف: ريم محمد عبيد
اسم المشرف: منذر مصطفى فتحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Markers have been described in type 1 diabetes mellitus (T1DM), There is a number of specific and non specific antigens have been identified. The major autoantigens involved in the destructive process of beta - cells leading to the development of type 1 diabetes are insulin hormone, glutamic acid decarboxylase (GAD), tyrosine phosphatase enzyme or Insulinoma - associated Antigen - 2(IA - 2).This study was conducted to find the relationship between antibodies for this antigens (insulin, GAD and IA - 2) and T1DM which could be used for the early detection of T1DM in normal Iraqi population. To study the importance of anti - thyroid peroxidase (anti - TPO) as a marker for autoimmune thyroid disease (ATD) in T1DM patients, and to find the relationship between T1DM and ATD.The study was carried out on 50 blood samples of men and 30 blood samples of women with age ranged from (20 - 60 years old), they were divided in to three groups : 1. Group 1 (20 men and 10 women) whom have fasting plasma glucose (FPG) above 180 mg/dL.2. Group 2 (20 men and 10 women) whom have FPG ranged from 120 - 180 mg/dL.3. Group 3 (10 men and 10 women) whom have FPG below 120 mg/dL.Blood samples were collected from all subjects, FPG and glycated haemoglobin (HbA1c) were measured. The levels of insulin, anti - insulin, anti - GAD, anti - IA - 2 and anti - TPO antibodies were measured in the serum.The statistical analysis results showed no significant difference in the presence of antibodies, HbA1c and FPG between men and women groups, inspite of some simple differences between the two groups.A significant (p<0.05) elevation in the level of FPG and HbA1c was observed in diabetic patients group compared withnon diabetic group. Significant (p<0.05) decrease in the level of insulin of T1DM patients was noticed compared to non diabetic group. Significant (p<0.05) elevation in the level of (anti - insulin, anti - TPO) in T1DM patients compared with non diabetic group. Elevation in the level of (anti - GAD, anti - IA - 2) in the T1DM patients compared with non diabetic group.The results also showed that no positive results for (anti - insulin, anti - TPO) present in the non diabetic group. One positive result for anti - GAD and one positive result for anti - IA - 2 present in the non diabetic group, which indicate the importance of anti - GAD and anti - IA - 2 antibodies ssay in normal population that could be used as early detection of T1DM.

دراسة تشريحية مقارنة لبعض الانواع البرية من ذوات الفلقتين النامية في محافظة بغداد Comparative Anatomy of Some Wild Dicots Spp. Grown In Baghdad Province

اسم المؤلف: زبيدة عبد اللطيف اسماعيل
اسم المشرف: علي حسين الموسوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present investigation dealt with comparative anatomical characters of 29 selected wild species that belongs to different families growing in different parts of Baghdad Province. These species are : 1. Brassica deflexa Boiss.2. Brassica rapa L.3. Cardaria draba (L.) Desv.4. Sinapis arvensis L.5. Sisymbrium irio L.6. Strigosella africana (L.) Boch.7. Stellaria media (L.)Vill.8. Atriplex nitens Schkuhr.9. Chenopodium album L.10. Chrysanthemum coronarium L.11. Lactuca serriola L.12. Sonchus oleraceus L.13. Medicago polymorpha L.14. Melilotus indicus (L.) ALL.15. Vicia sativa L.16. Erodium cicutarium(L.) L'H17. Geranium rotundifolium L.18. Lamium amplexicaule L.19. Malva parviflora L.20. Plantago lanceolata L.21. Polygonum aviculare L.22. Rumex dentatus L.23. Ranunculus muricatus L.II24. Veronica polita Fries.25. Astrodaucus leptocarpus (Hoghst.) H. Riedl26. Urtica urens L.27. Verbena officinalis L.28. Lippia nodiflora (L.) Rich.29. Tribulus terrestris L.Some of these species have been anatomically investigated for the first time.Anatomical characters might be useful for plant biologist for the identification of important wild plants as an additional character at global level. Comparative anatomical characters were used; such as characteristics of ordinary epidermal cells of stems and leaves and stomatal complexes. The epidermis possesses number of important diagnostic character that offer valuable clues for identification, like size, shape, in addition to indumentum of both stems and leaves. Venation system was investigated and compared. Cross sections of root were useful taxonomically especially the thickness of epidermis, cortex, phloem and xylem. The study observed presence of sclerenchyma tissues in the root cortex of some species. Cross sectioning of stems and petioles were also studied. Epidermis, cortex, pericycle, vascular bundle shapes and numbers, pith characters, presence of crystals and tannin filled cells were useful aid in distinguishing species. Vertical sections of leaf blades, shape and number of vascular bundles, thickness of palisade and spongy layers and other mesophyll characters were important taxonomically.ccording to some of these characters, species were divided into groups. This study concluded the presence of variations in characters and these were presented for the first time comparatively, so that, the anatomical characters were a good support to the exomorphological characters in the studied species. Field photographs of the different studied plants and sections of organs were put in addition to numerous tables in this work. The above results were discussed scientifically regarding mainly the environmental factors.

فعالية مستخلصات بعض الطحالب الكبيرة للفطريات الممرضة للنبات Activity of Some Macro - Algae Extracts Against Phytopathogenic Fungi

اسم المؤلف: دنيا يوسف محمد يوسف
اسم المشرف: عبد اللطيف محمد جواد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص طحالب كبيرة من بيئتين مائيتين محليتين مختلفتين. تم عزل ثلاث طحالب كبيرة هي Cladophora glomerata (N) وEnteromorpha rafsii من منطقه بحر النجف في محافظة النجف, في حين عزل Cladophora glomerata (R) من احد مبازل منطقة الراشدية ش | The present study includes isolation and identification of Macro - algae from two different environmental water bodies. Three Macro - algae were isolated, Cladophora glomerata (N) and Enteromorpha ralfsii from Baher Al - Najaf region in Holy Najaf city. In addition, C. glomerata (R) was isolated from Al - Rashdiya, north of Baghdad.Phytopathogenic fungi from soil and some infected fruits were also isolated. They were identified as Pythium ultimum and Rhizoctonia solani.Water and ethanol were used to extract each algae to evaluate their antifungal activity against isolated phytopathogenic fungi. Different concentrations of these algal extracts had been prepared which are (10, 25 and 50) mg/ml and the antagonistic activity against the isolated phytopathogenic fungi were evaluated, the hot and cold water extracts did not show any antagonistic activity against fungi. However, all these extracts were ignored in the future work for this study. Furthermore, the hot ethanolic extract was more efficient than these of cold ethanolic extracts. Results have been shown that there were significant differences when macro - algal ethanolic extract were used.C. glomerata (R), which isolated from Al - Rashdiya, was more efficient against tested fungi than the same alga what isolated from Baher Al - Najaf region. However, E. ralfsii extract was more efficient against tested fungi than C. glomerata (N). Percentage of inhibition against P. ultimum when hot ethanolic extract of E. ralfsii, C. glomerata(R) and C. glomerata(N) were (88.8, 83.3 and 63.3) respectively.However, results for cold ethanolic extract of the same algae were (63.3, 52.2 and 32.2) respectively when 50 mg/ml of algal extracts were used.Percentage of inhibition against R. solani when hot ethanolic extract of E. ralfsii, C.glomerata (R) and C.glomerata(N) were (94.4, 100 and 78.8) respectively.However, results for cold ethanolic extract of the same algae were (66.6, 64.4 and 48.8) respectively when 50 mg/ml was used. According to the difference in biological activities against those phytopathogenic fungi caused by the same species of macro - algae or different genera from different environments. Due to difference in biological activity of the same macro - algae species Physiochemical parameter for each environments obtained such as air and water temperature, Turbidity (NTU), Electrical Conductivity(EC)and Salinity%, Water pH, CA+2 concentration, Mg+2 concentration, Total Hardness and Total Alkalinity. Results indicated that soaked Cucumis sativus and Capsicum annum seeds in cold and hot ethanolic macro - algae extracts of (E. ralfsii, C. glomerata (R) and C.glomerata (N) for 24 hours were able to protect seed germination that have been grown in a Petri dish contaminated with P. ultimum and R. solani comparing with control. In addition, results indicated that spraying C. sativus and C. annum seedling with these algal extracts within two weeks old again protects these seedlings from the same phytopathogenic fungi either before or after 24 hr. However, results indicated that adding the algal extracts within two months age to the C. sativus and C. annum plants again protects these plants from the same phytopathogenic fungi comparing with control and fungicide treatment. Moreover, results indicated that the active chemical compounds in E. ralfsii was Tannins, Saponins and Flavonoids. While C. glomerata was contained Tannins, Saponins, Alkaloid and Phenols. Results indicated that both hot crud methanolic extract of C. glomerata (R) and E. ralfsii have a lot of active chemical compounds against micro - organisms by using the GC - Mass Spectrometry technology.

التحري عن بعض عوامل الضراوة للزائفة الزنجارية المعزولة من الحليب الخام والجبن الطري Detection of Some Virulence Factors of Pseudomonas Aeruginosa Isolated From Raw Milk And Soft Cheese

اسم المؤلف: عبد الكریم كاظم عبد الحسین الحسب
اسم المشرف: علي حسن احمد الشمري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: صحة الغذاء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In order to detection of Pseudomonas aeruginosa in food - chain especially from Cows raw milk and soft cheese with its whey, this study was designed in some regions of Baghdad (College of Veterinary Medicine, Abu - Ghraib, Al - Sadrya & Al - Radwaniyah), in which a standard isolation methods were used with some modification processing by new, modern and rapid technology tools such as chromogenic medium CNP agar and Electronic rapid Microbact TM 24E panel identification system supported by standard color differential chart and online American Type Culture Collection (ATCC) Codes; aids in rapid and precise identification, differentiation, confirmation and enumeration of P. aeruginosa isolates from raw dairy samples; as well as, detection of biofilm producing versatile morphotypes with chameleon phenomenon and fruity odour; and demonstration the activity of food spoilage enzymes (protease, lipase & lecithinase) of isolates with thermo - stability and viability in different temperatures with bitty cream and ropy fermentation phenomenon. The results profile showed : 1. Isolation of 46 (76.7%) isolate of P. aeruginosa from a total of 60 Cow Dairy samples : 30 Raw Milk samples (pooled from milk cans and apparently healthy Cows with a history of mastitis cases) as 19 (31.7%) isolates from regions of College of Veterinary Medicine, Abu - Ghraib and Al - Sadrya (10 from each region); and 30 Soft Cheese with its Whey samples (pooled from unhygienic processed cheese and containers in unclean dirty environment and apparently carrier or diseased workers) as 27 (45%) isolates from regions of Abu - Ghraib, Al - Sadrya and Al - Radwaniyah (10 from each region). Isolation percentages of oxidase positive P. aeruginosa from selected Regions in Baghdad Province indicates significant differences among regions at a level (P?0.05), in which highest isolation percent of P. aeruginosa from Abu - Ghraib as 20 (33.4%) isolates (10 isolates from raw milk and 10 isolates from soft cheese) in accordance to 17 (28.4%) isolates from Al - Sadrya (9 isolates from raw milk and 8 isolates from soft cheese), 9 (15%)SUMMARYisolates from Al - Radwaniyah (9 isolates from soft cheese) without any isolate from College of Veterinary Medicine, which may indicate good hygienic measurements.2. Segregation of isolates into two Haemolytic Patterns : Livestock type origin, lysis only sheep blood (L - type) as 19 (41.30%) isolates from raw milk samples; and Zoonotic type lysis, both blood (Z - type) as 27 (58.70%) isolates from soft cheese with its whey as high prevalence due to critical processing chain, risky carrier individuals and contaminated environment or polluted water supply. This may indicate indirectly genetic diversity and host or cross individual tropism in haemolysins - phospholipases activity according to different types of samples, regions and hosts.3. The study revealed isolation and segregation of versatile morphotypes and five pigments with fruity odor (Chameleon phenomenon) with mucoid drippy due to alginate or small colonial variants smooth or rough or both especially in isolate code M7 from mastitic milk and isolate code C27 from contaminated soft cheese and its whey. Wrinkled - Corroded green morphotypes were more evident in blood agar due to secretion of siderophore pyoverdine in order to chelating iron for activation of isolate. Motility Pattern with TTC salts was evident in all isolates with three types of motility : swarming, swimming and twitching, that noticed obviously in M7 isolate.4. New technology biochemical identification panel system (Microbact TM 24E) confirm that isolates were P. aeruginosa at level (P?0.01) after online matching with standard Remel ATCC Octalcodes bank of P. aeruginosa, in which 5 octalcodes typical and atypical were documented, that indicate genetic diversity in biochemical reactions of isolates and their morphotypes, modified microbial gene sharing protocol of P. aeruginosa with other bacteria and highly Sensitivity and Specificity of the test panel.SUMMARY5. Detection and isolation of Biofilm producing isolates by five methods, in which microtiter plate assay was the most sensitive and realizable technique while Congo red agar test detect quantity and type of biofilm production through colour of colonies (black very strong and huge, gray strong, pink moderate and white fair or non - producer). Biofilm produced in most morphotypes with pellicles at liquid interphase and ropy viscous threads (sliminess) and bitty cream especially in isolate codes M7 and C27. Small colonial variants of some morphotypes can revert to mucoid drippy variants. M7 showed unusual ropy thread of 30 cm long.6. The results showed secretion of thermo and psychro stable and labile food spoilage enzymes (protease, lipase and lecithinase) from isolates, in which versatile morphotypes showed indirectly diverse genetic ability, this may indicate variation of thermal tolerance mechanisms (heat shock proteins and biofilms formation) in isolates in accordance to time and temperature of processing, that linked genetically with the quorum sense mechanisms of acyl homoserine lactone gene regulatory region (AHL) in P. aeruginosa isolates (Stress Hardening phenomenon).7. The results showed the genetic ability (indirectly) of some isolates to resist, tolerate and proliferate in acidic environment at pH 2 & 4 especially isolate M7 & C27, while intermediate to susceptible tolerance of pH were noticed in others. This may indicate the power of electromagnetic charged net field of complex polymeric matrix of alginate exopolysaccharide layers in protecting some isolates from adverse acidic environment due to quorum sensing behavior of M7 & C27.Therefore, it could be concluded from this study that P. aeruginosa was prevalent in food chain especially from Cows raw milk and soft cheese with its whey samples from some regions in Baghdad, and Biofilm producing isolates with their thermo and psychro stable enzymes complex system and their tolerance to acidic environment stressors were more dangerous thus, we recommend monitoring these products periodically for insurance of public health.

تقدير الظواهر الفمويه والعوامل المناعيه في اللعاب عند مرضى سرطان الغدد اللمفاويه قبل وبعد العلاج الكيميائي Assessment of Oral Manifestations And Salivary Immunological Markers In Patients With Lymphoma Before And After Receiving Chemotherapy

اسم المؤلف: محمد حسن عبد الشهید
اسم المشرف: فواز داوود الاسود
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: دبلوم عالي
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Lymphomas are group of diseases caused by malignant lymphocytes that accumulate in lymph nodes and caused the characteristics lymphadenopathy.Occasionally, they may spill over into blood or infiltrate organs outside the lymphoid tissue The major subdivision of lymphomas is into Hodgkin's lymphoma and non - Hodgkin's lymphoma and this is based on the histologicpresence of Reed - Sternberg cells in Hodgkin's lymphoma. Aims of the study : The aims of this study were to determine the percentage of oral manifestations and identify the levels of salivary immunoglobulin A, interluekin - 6 and tumor necrosis factor - ? in lymphoma patients before the treatment and after receiving three cycles of chemotherapy in comparison with healthy control individuals.Subjects, materials and methods : The study included 25 patients( 15 male and 10 female ) with non - Hodgkin's lymphoma (B - cell type), 25 patients( 16 male and 9 female ) with Hodgkin's lymphoma and 25 ( 15 male and 10 female ) healthy control group with no signs and symptoms of any systemic disease and age, sex match with patient groups.Those patients were examined pre and post chemotherapy to see the oral manifestations and compared with healthy control group. Whole unstimulated saliva was collected to determine the level of salivaryimmunological markers which measured by Enzyme Link Immunosorbent Assay.IVResults : The percentage of oral manifestations was taste alteration in non - Hodgkin's lymphoma patients ( 36% ) and in Hodgkin's lymphoma patients ( 16% ) , followed by burning mouth syndrome ( 20% ) in non - Hodgkin's lymphoma patients and ( 16% ) in Hodgkin's lymphoma patients and lastly dry mouth was ( 12% ) in both non - Hodgkin's lymphoma and Hodgkin's lymphoma groups. In the present study there was no oral manifestations in both study groups before starting chemotherapy, and the mentioned oral manifestations incident after receiving chemotherapy regimens. The levels of salivary immunoglobulin A, interluekin - 6 and tumor necrosis factor - ? were significantly higher in pre - treatment patients in comparison with control group, and there was a significant decrease after chemotherapy treatment when compared to their base line levels in both study groups.Conclusions : The most frequent oral manifestations in lymphoma patients was taste alteration followed by burning mouth syndrome and dry mouth , and male patients affected more than females.The salivary immunological markers levels were higher in lymphomapatients than control, then these markers showed obvious decrease in patients after chemotherapy treatment

تاثير مستخلصات الشاي الاخضر على مسببات امراض انسجة الاسنان الرابطة (بكتريا البورفايروموناس اللثوية وبكتريا اجريجاتيباكتر اكتينومايستيمكومتانس) : دراسة مختبرية Antibacterial Effects of Green Tea Extracts On Periodontal Pathogens (Aggregatibacter Ctinomycetemcomitans And Porphyromonas Gingivalis) (In Vitro Study)

اسم المؤلف: ميس جمال مجيد
اسم المشرف: سيف سهام سليم
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: امراض ما حول الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقدمة : الشاي الاخضرهو واحد من المشروبات العلاجية القديمة والشعبية المستهلكة في جميع انحاء العالم، وهو مشتق من ورقة للنبات "كاميليا سينينسيس", يمكن ان يحضر كمشروب، والذي يمكن ان يكون له العديد من الاثار الصحية الشاملة او "مستخلص" يمكن الحصول عليه من الاو | Background : Green Tea is one of the most ancient and popular therapeutic beverages consumed around the world, it is made from the leaf of the plant “Camellia sinensis”. It can be prepared as a drink, which can have many systemic health effects or an “extract” which can be made from the leaves to be used as medicine. Green tea is reported to contain thousands of bioactive ingredients including catechins, catechins found in the tea have shown promise for having antimicrobial effects.Aim of study : to test the effect of green tea extracts on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in comparison to 0.2% chlorohexidine gluconate and distilled water in vitro.Materials and Methods : plaque samples were collected from 20 patients with no signs of any systemic disease suffering from chronic periodontitis with probing pocket depth of at least 6 mm, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were isolated and diagnosed according to morphological characteristic and biochemical test. Green tea leaves were extracted by using water and alcohol. This study involved two experiments in vitro concerning the effects of green tea extracts on these bacteria in addition to analysis of the extracts to determine the concentrations of the catechins in each extract. The first experiments involved testing the sensitivity of A.a and P.g to different concentrations of the extracts in addition to chlorhexidine gluconate 0.2% and distilled water using agar well diffusion method, the second experiment involved determination of the minimum inhibitory concentration of the extracts that inhibits the bacterial growth and then determination of the minimum bactericidal concentration of the extract that was requires to kill the bacteria. The present study also involved laboratory analysis of green tea extracts using high performance liquid chromatography (HPLC).Results : Both green tea extracts were effective in inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans using agar well diffusion method, 90% and 100% concentrations of alcoholic extract showed larger inhibition zones than chlorhexidine gluconate 0.2% with statistically significant difference, CHX showed higher inhibition zones than all aqueous extract concentrations.The MIC (minimum inhibitory concentration) of alcoholic green tea extract that inhibit Aggregatibacter actinomycetemcomitans growth was 60% and it was the same for Porphyromonous gingivalis.The MIC of aqueous green tea extract that inhibits Aggregatibacter actinomycetemcomitans growth was 70% and MIC of aqueous green tea extract extract that inhibits Porphyromonous gingivalis was 80%.The MBC (minimum bactericidal concentration) of alcoholic green tea extract that kills Aggregatibacter actinomycetemcomitans was 80%, the MBC of alcoholic green tea extract that kills Porphyromonous gingivalis was 90%, the MBC of aqueous green tea extract that kills Aggregatibacter actinomycetemcomitans growth was 90%, which was also the same for Porphyromonous gingivalis.HPLC analysis of aqueous and alcoholic green tea extracts that were used in this study revealed that alcoholic extract contained higher concentration of EGCG while aqueous extract had higher content of catechin and epicatechin.Conclusion : green tea extracts were effective against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, alcoholic green tea extract showed inhibition ability more than the aqueous green tea extract and more than chlorhexidine gluconate and it showed bactericidal activity at 80%, 90% and 100% concentrations.

الطريقة السريرية للتنبؤ بكثافة العظام السخية في المنطقة الواقعة بين الضاحك الثاني والطاحن الاول في البالغين العراقيين اصحاب الاطباق الطبيعي Clinical Method For Prediction of Alveolar Bone Mineral Density In The Area Between The 2Nd Premolar And 1St Molar In Iraqi Adults With Class I Occlusion

اسم المؤلف: مها علي حسن الجبوري
اسم المشرف: هديل علي حسين الهاشمي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Orthodontic mini - implants are increasingly used in orthodontics and the bone density is a very important factor in stabilization and success of mini - implant.The aim of this study was to observe the relationship among maximum bite force; body mass index; face width, height; masseter muscle length, width and thickness; and bone density in an attempt to predict bone density from these variables to eliminate the need for CT scan which have a hazard on patient.Computed tomographic images were obtained for 70 patients (24 males and 46 females) with age range 18 - 30 years. The cortical and cancellous bone density was measured between maxillary and mandibular 2nd premolar and 1st molar at two levels from the alveolar crest (3 and 6 mm). Face height and width and masseter muscle length, width and thickness were measured from CT. Clinically; Maximum bite force was measured on first molar region unilaterally by a digital device. The sample was divided into two groups according to the body mass index into; normal and overweight.The results that were obtained showed that there were no significant differences in bone density or maximum bite force in both genders. Only the cortical bone density in maxilla in overweight group tended to be higher than normal body mass index group. The face width and height correlated significantly negatively with or maximum bite force which correlated significantly positively with cortical bone density and masseter muscle width.It was concluded that a prediction of cortical bone density of preselected areas can be made from maximum bite force, body mass index and inter - zygomatic width

التقييم النسيجي والميكانيكي للتكامل العظمي لزرعات التتانيوم بعد تحرير تصميم الجزء المسنن من الزراعة او طلائها بمادة بذور الكتان : دراسة تجريبية على الارانب Histological And Mechanical Evaluation of The Osseointegration of Titanium Implants By The Modification of Thread Design And/Or Coating With Flaxseed (An Experimental Study On Rabbits)

اسم المؤلف: مصطفى هادي جواد
اسم المشرف: عذراء يحيى الحجازي نبيل كاظم عبد الصاحب
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: الانسجة الفموية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Dental implant surface technologies have been evolving rapidly to enhance a more rapid bone formation on their surface and hold a potential to increase the predictability of expedited implant therapy.Numerous surface modification approaches have been developed for all classes of dental implants to modulate biological responses and improve the osseointegration and primary stability. Hence, Implant threads should be designed to maximize the delivery of optimal favorable stresses while minimizing the amount of extreme adverse stresses to the bone implant interface. In addition, using of biomaterial coating of implant surface for the enhancement of bone formation has been achieved through the modulation of osteoblasts adhesion and spreading that induced better stability and more implant surface contact area.The aims of the study are : 1. Evaluation of titanium implant osseointegration by the modification of thread design and I or coating its surface by Flaxseed.2. Evaluate the new surface modification of titanium implant, histologically, mechanically and radiographically.Materials and methods : One hundred and twenty eight commercially pure titanium (CpTi) screws were implanted in rabbit tibiae. Implants were divided as follows : 1. Dual - threaded group (32 implants). 2. Single - threaded coated with grinded flaxseed group (32 implants).3. Single - threaded with central groove (32 implants).4. Single - threaded (control) group (32 implants).Histological, mechanical and radiographical analysis were performed for all implants after each healing periods (2,4,6 and 8) weeks.Results : 1. Dual - threaded implant illustrated the highest mean value in thread width and removal torque, and it showed mature bone at 8 weeks.2. Grooved implant illustrated close opposing threads. 3. Coated implant illustrated highest mean value in osteoblast cell count and showed a proliferating cartilage zone that later on degenerated and replaced by bone.Conclusion : The present results conclude that each modified implants shows a different benefits whether a modification of the implant surface mechanically (dual or grooved thread) or by coating the implants with Flaxseed.

الظهور الكيميائي النسيجي المناعي للاوستيوكالسين، OC عاملا لنمو المحول بيتا واحد TGF - B1 وبروتين الشكل العظمي سبعة BMP - 7 في خلل التنسج الليفي والورم الليفي المعظم في عظام الفك : دراسة مقارنة Immunohistochemicalexpression of Osteocalcin, Transforming Growth Factor Beta - 1And Bone Morphogenetic Protein - 7 In Fibrous Dysplasia And Ossifying Fibroma of The Jaw Bones Acomparative Study

اسم المؤلف: فرح غسان ابراهیم
الموضوع العام: طب الاسنان
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Fibrous Dysplasia & Ossifying Fibroma of the jawsare maxillofacial fibroosseous lesions sharing anoverlapping clinicopathological characteristics. This can be diagnostically challenging for pathologists& surgeons. It is important to make the distinction because of differences in their clinical behavior, modes of treatment and prognosis.Osteocalcin is an immunohistochemicalmarker; secreted by osteoblasts and its high serum levelsare correlated with increased bone mineraldensity. It is therefore, used as biomarker for boneformation process and also has a role in regulationof osteoblast function.Transforming growth factor beta - 1 is a multifunctional regulator of cell growth which will either stimulate or inhibit proliferation of mesenchymal cells depending on the presence of other growth factors. It is secreted by osteoblasts and is very abundant in bone matrix. Bone morphogenetic protein - 7is a member of transforming growth factor - b superfamily; it is widely expressed during embryonic growth, and is an essential morphogen in renal, skeletal, and eye development.Aims of the study : This study aimedto evaluate and compare the Immunohistochemical expression ofosteocalcin, transforming growth factor beta - 1 andbone morphogenetic protein - 7in the pathogenesis of Fibrous dysplasia and Ossifying fibroma of the jaw bones,correlate the expression of the aforementioned markers in these lesions.Analyze theimmunohistochemical expression ofosteocalcin, Transforming growth factor beta - 1 & bone morphogenetic protein - 7 inFibrous dysplasia & Ossifying fibroma, in order to assess its potential role in differentiation between these two disease entities.Materials&Methods : A total of 30 retrospective formalin - fixed, paraffin - embedded tissue blocks were included in this study, 15 were diagnosed asFibrous dysplasia of the jaws and 15 were ofOssifying fibroma of the jaws. Animmunohistochemicalstaining method using ofosteocalcin,transforming growthfactor beta - 1 andbone morphogenetic protein - 7 monoclonal antibodies were performed.Results : The results revealed that the majority of cases (73.33%) were females for each Fibrous dysplasia &Ossifying fibroma. the age range was (8 - 35)years for Fibrous dysplasia and (7 - 50)years forOssifying fibroma. For Fibrous dysplasia most cases presented in maxilla (66.76%) while for Ossifying fibroma most of the cases presented in mandible (73.33), with more predominant Fibrous dysplasia cases in molar area (60%) and more presented Ossifying fibroma cases in premolar & molar area (33.33%). Statistically significant difference was found between Fibrous dysplasia&Ossifying fibroma cases regarding jaws & site distribution (P=0.02&0.04) respectively.Osteocalcin positive immunohistochemicalexpression was found in fibroblast - like cells in 4 cases(26.66%) of Fibrous dysplasia and in 7 cases (46.67%) of Ossifying fibroma, according to Chi - square test the result showed statistically significant difference regarding osteocalcin expression in Fibrous dysplasia &Ossifying fibroma (P=0.04). Transforming growth factor beta - 1 was positivelyexpressed in 8cases (53.3%) of Fibrous dysplasiaand 10 cases (66.67%) of Ossifying fibroma. Bone morphogenetic protein - 7showed positive expression in 2 cases (13.3%) of Fibrous dysplasia and 4 cases (26.7%) ofossifying fibroma.Statistically non - significant difference regarding the immunohistochemicalexpression of transforming growth factor beta - 1 and bone morphogenetic protein - 7in Fibrous dysplasia &Ossifying fibroma. Moreover significant correlation was found regardingtransforming growth factor beta - 1 expression in response to bone morphogenetic protein - 7in Fibrous dysplasia (P=0.03)and osteocalcin expression in response totransforming growth factor beta - 1 in Ossifying fibroma (P=0.01).Conclusions : Transforming growth factor beta - 1 expressin the majority of Fibrous dysplasia &Ossifying fibroma cases indicate its important role in the process of osteogenesis. The significant correlation seen regarding the expression of some of the studied markers with each other suggest their cooperative role in the pathogenesis of Fibrous dysplasia &Ossifying fibroma

الظواهر الفمية، مؤشرات عامل الغلوبولين المناعي اللعابي A، الانترلوكين واحد بيتا وعامل تنخر الورم الفا في لعاب مرضى - الكلى المزمن الخاضعين للغسل الكلوي في محافظة ميسان Oral Findings, Salivary IgA, Interlukin - 1 Beta And Tumor Necrosis Factor Alpha Markers In Saliva of Chronic Kidney Disease Patients Undergoing Hemodialysis In Missan Governorate

اسم المؤلف: فارس عبد حاتم
اسم المشرف: زاهدة جاسم محمد
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Chronic kidney disease refers to an irreversible deterioration in renal function which classically develops over a period of years, the ageing of populations along with the growing prevalence of chronic diseases such as diabetes and hypertension is leading to worldwide increase in the number of chronic kidney disease patients, the patients suffering from chronic renal failure face oral health related problems because it affects the bone and soft tissue structures.During hemodialysis, blood contact with a foreign surface, such as a complement - activating dialytic membrane, promotes a variety of complex and interrelated events, leading to an acute inflammatory response.Cytokines are a broad and loose category of small proteins that are important in cell signaling, they are released by cells and affect the behavior of other cells, they are important in health and disease, specifically in host responses to infection, immune responses, inflammation, trauma, sepsis, cancer and reproduction.Aims of study : The aims of this study was to determine the prevalence of oral findings, salivary flow rate and evaluation of salivary immunoglobulin A, interleukin - 1? and tumor necrosis factor ? levels in chronic kidney disease patients on hemodialysis treatment in comparison with healthy control, and investigate if there is a relation between these salivary parameters (salivary flow rate, salivary immunoglobulin A, interleukin - 1? and tumor necrosis factor ? levels) in chronic kidney disease patients on hemodialysis (less and more than one year) and finally find the correlation between salivary parameters in patients group.Methods : Ninety (90) subjects were participated in this study, they were divided into two groups : Patients group comprised of 45 subjects undergoing hemodialysis with chronic kidney diseases; Control group comprised of 45 subjects with no history of any systemic diseases.Intra oral examination was done for each individual to record the prevalence of oral findings in chronic kidney diseases patients. Saliva collected was measured and levels of salivary immunoglobulin A, interleukin - 1? and tumor necrosis factor ? were measured by enzyme linked immunosorbent assay (Elisa).Results : The most frequent oral findings in chronic kidney disease patients on hemodialysis, in this study were dry mouth (77.7%), uremic breath (bad odor) (55.5%) , taste alteration (55.5%), angular chelitis (35.5%), mucosal pallor (26.6%), burning mouth syndrome (28.8%), staining of teeth (26.6%), oral ulceration (11.1%) and petechia and ecchymosis (2.2%), and the present study showed that no statistically significant differences (p>0.05) were found in oral findings distribution except angular chelitis which showed statistically significant difference (p<0.05) between chronic kidney diseases patients on hemodialysis for less than one year & for more than one year. According to salivary flow rate, the present study found that there was a significant difference in patients with chronic kidney disease in comparison to healthy control subjects, also statistically there was no significant difference in salivary parameters (salivary immunoglobulin A, interleukin - 1? and tumor necrosis factor ? and salivary flow rate) in chronic kidney diseases patients on hemodialysis for less than one year & for more than one year. The present study showed that there was no correlation between salivary parameters (salivary immunoglobulin A, interleukin - 1? and tumor necrosis factor ? and salivary flow rate) except salivary immunoglobulin A and interleukin - 1? showed positive significant linear correlation (r=0.368, p=0.006) and negative significant linear correlation between salivary immunoglobulin A and salivary flow rate (r= - 0.275, p=0.009).Conclusions : Cytokines may play roles in pathogenesis of chronic kidney diseases represented by increasing interleukin - 1? and tumor necrosis factor ? levels in saliva of those patients, the chronic kidney diseases patients on hemodialysis recommended comprehensive professional oral care and self - care instructions

العلامات الالتهابية اللعابية وعلاقتها بحالة الفم الصحية بين مجموعة من الصبيان الذين يعانون من السمنة بعمر 21 سنة Salivary Inflammatory Biomarkers In Relation To Oral Health Status Among Obese Boys Aged 12 Years

اسم المؤلف: عاتكة رسول عزيز
اسم المشرف: احلام طه محمد
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان الوقائي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Worldwide, obesity trends are causing serious public health concerns and significantly increases the risk of morbidity and mortality. It has an effect on the person`s general health including the oral health. Aims of the study : This study was designed to investigate the oral health status among a group of obese boys in relation to salivary inflammatory biomarkers (interleukin - 6 and Creactive protein) and selected salivary elements.Materials and methods : The study group consists of forty obese boy of an age 12 year, and forty normal weighted boy of the same age as a control group. Assessment of nutritional status was done by using Body Mass Index specific for age and gender. Dental caries severity was recorded by using the index of Muhlemann (1976). Plaque index of Silness and Loe (1964) was used for plaque assessment, calculus component of periodontal disease index of Ramfjord (1959) was used for calculus assessment and gingival condition assessed according to gingival index of Loe and Silness (1963). Unstimulated salivary samples were collected under standardized condition, salivary flow rate was determined and then the salivary samples chemically analyzed to determine the concentration of salivary interleukine - 6, Creactive protein, calcium, magnesium and phosphorus.Results : Caries experience of permanent dentition (DMFS) among study group was lower than that among control group with significant difference (P<0.05) for D2, while caries experience of primary dentition (dmfs) was significantly (P<0.05) lower among study group compared with control group with significant difference (P<0.05) for ds and highly significant difference (P<0.01) for d4.Salivary flow rate was lower among study group compared with control group with no significant difference. No significant correlations (P>0.05) were recorded between salivary flow rate and dental caries, oral cleanliness and gingivalhealth conditions among both groups. The mean value of plaque, calculus and gingival indices were not significantly lower among study group compared with control group. A positive highly significant correlations (P<0.01) were recorded between gingival and plaqueindices among both groups. The level of salivary interleukin - 6 (pg/ml) was higher among study group compared with control group with no significant difference between them; whereas there was no significant difference between the two groups regarding C - reactive protein (?g/ml) level. Salivary calcium and magnesium levels (mg/dl) were highly significantly (P<0.01) lower among study group compared with control group, whereas salivary phosphorus level (mg/dl) was not significantly higher among study group compared with control group. Salivary interleukin - 6 and C - reactive protein were negatively correlated with dental caries of both dentitions among study group with significant correlation (P<0.05) between IL - 6 and D2, while they were correlated positively with dental caries of both dentitions among control group with highly significant correlation (P<0.01) between IL - 6 and D3 and significant correlation (P<0.05) between IL - 6 and d4. Salivary calcium was correlated positively highly significantly (P<0.01) withDMFS and significantly (P<0.05) with D4 among control group. Salivary magnesium was correlated positively significantly (P<0.05) with DMFS and D2 among study group; also it was correlated negatively highly significantly (P<0.01) with d3 amongcontrol group. Salivary phosphorus was correlated significantly (P<0.05) in positive direction with d3 among study group; also it was correlated highly significantly (P<0.01) in negative direction with DMFS and significantly (P<0.05) in positivedirection with d3 among control group.

اثر التطبيق الموضعي من الكركمين مسحوق وزيت اساسي على التئام جروح الجلد : دراسة نسيجية وكيميائية نسيجية مناعية في الارانب Evaluation The Effect of Topical Application of Curcumin Powder And Essential Oil On Skin Wound Healing In Rabbits (Histological And Immunohistochemical Study On IGF - 1R)

اسم المؤلف: نضال حسين محمد
اسم المشرف: بان عبد الغني جميل
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: الانسجة الفموية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: خلفية الموضوع : طب الاعشاب يمكن ان يعرف كواحد من فروع الطب باشكال مختلفة. وقد اثبت كركمين الكركم فعاليته كماده ملونة، ومنكهة واستخدم تقليديا في الطب،فقد اظهر له خصائص ملحوظه مضادة للالتهابا ت، مضادات الاكسده وخصائص بيولوجية متنوعة.ان عدم وجود سمية للكركم | Back ground : Herbal medicine can be called one of the branches of medicines in various forms.Turmeric curcumin has proved it's efficiencies a coloring, flavoring agent and has been traditionally used in medicine, exhibiting remarkable anti - inflammatory, and antioxidant properties. The varied biological properties of curcumin and lack of toxicity even when administered at higher doses makes it attractive toexplore its use in various disorders like diseases of skin.It is good potential agent for wound healing.Considerable interest has been focused on curcumin compound, mechanisms that drive wound repair are complex and have challenged wound - healing investigators for many years.Aims of the study : To evaluate the effects of topical application of curcumin (powder and essential oil)on skin wound healing histologically and immunhistochemically.Materials and methods : Sixty four New Zealand rabbits were used in this study, they were divided into four groups (16 animals for each healing period)each group was subdivided as follows : - Experimental groups(8 rabbits)subdivided into : a - Right facial side of(eight rabbits animal) for essential oil application Left facial side of(eight rabbits animal)for curcumin powder application - Control group (eight rabbits)(right facial sides used as control). Histological evaluation of wound healing was performed for all studied groups.Imunohistochemical analysis was utilized to localize and characterizethe IGF - R1 immuno positive cells at the wound site of allgroups (experimental and control).Results : Histological findings of the present study have shown that reepithelialization, wound contraction were accelerated after topical application of curcumin and essential oil. The results ofimmunohistochemical examination of this study revealed that there was increased expression of IGF - 1R by epithelial and connective tissue cells of skin especially with the topical application of curcumin oil at wound site.Conclusion : - Essential oil is more effective in enhancement of wound healing regarding histological and immuonohistochemical assessment revealed higher mean values of positive expression for insulin growth factor1receptor(IGF - 1R) , (for both stromal and epidermal).

دراسة نسيجية , شكليه نسيجيه ونسيجية مناعية للاستخدام الموضعي ل VEGF / Collagen I في تجويف الاسنان المقلوعة : دراسة تجريبية على الجرذان Histological, Histomorphometrical And Immunohistochemical Evaluation of Local Application of Collagen I And /Or Vascular Endothelial Growth Factor (An Experimental Study In Rats

اسم المؤلف: رفل راشد عبد السادة
اسم المشرف: عذراء يحيى الحجازي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: امراض ما حول الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Back ground : Healing socket consists of three new tissue components; epithelium, connective tissue, and bone tissue. Interactive dynamic changes take place between these 3 components during healing period.During the process of healing of the extraction socket the following events occur : firstly, a blood clot (mainly blood cells and network of fibrin) forms and fills the empty socket. Then, the blood clot matures and is organized by the formation of granulation tissue (rich in newly formed vascular structure, abundance of inflammatory cells. The granulation tissue replaces the blood clot completely by the seventh day. After 20 days, the granulation tissue was replaced by collagen, and bone began forming at the base and the periphery of the extraction socket. Epithelium covers the newly formed bone that filled the socket.The healing cascade of all wounds ultimately requires neovascularization, collagen deposition, and collagen constriction by myofibroblasts. Neovascularization in the adult is known to occur by two distinct processes, angiogenesis and vasculogenesis which needs for expression of VEGF.Aim of the study : Histological ,Histomorphometrical and immunohistochemical studies to evaluate the effect of local application of exogenous VEGF/collagen I separately and as a combination in socket healing.Materials and Method : Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1st molar of both sides ( right side was considered as experimental site ,while left be the control one).The animals were divided into following groups according to the applicable of biomaterials.A. Control group the tooth socket treated with 1?L of normal salineB. Experimental group includes• Group I contains (20) rats, the tooth socket treated with 1?L of VEGF• Group II contains (20) rats the tooth socket treated with 1?L ofcollagen type I• Group III contains (20) rats, the tooth socket treated with 1?L of a combination of VEGF and collagen I. Each group is composed of 20 rats that will be studied in four periods 3,7,14,28 days (5 rats for each period).The specimens were studied histologically histomorphometrical and immunohistochemical identification of fibroblast growth factor 2 (FGF2) and tissue non specific alkaline phosphtase (ALP).Results : 1. Histological findings for all groups illustrate formation of blood clot at 3 days.2. Granulation tissue appears in all groups at 7 days period, and new bone is demonstrated in collagen group and a highly cellular woven bone with active proliferative osteoblast and newly blood vessel is detected in combination group.3. At 14 days new epithelization and new bone trabeculae with fibrous tissue are presented mostly in all groups but with different constitution.4. At 28 days all groups show reepithelization but in different thickness, and with newly bone apposition and with different maturity.5. For positive cells expressed ALP VEGF group records a high mean values at 3,14,28 days periods and with high differences in comparisum to other groups while control group reports a high mean value at 7 days.6. For positive cells expressed FGF2.Control group illustrates a high record for the mean of positive cells expressed FGF2 at 3,7days periods and with high differences in comparisum to other groups, while combination group reports a high mean value at 14 days.7. Results demonstrated that in most periods for each group ,whenever ,ALP is a high value in expression ,records a low expression in FGF2.Conclusion : Results ,high lighted on the effect of local application of VEGF in extracted tooth socket that facilited epithelization ,while combination of (Collagen and VEGF ) shows a high mineralization zone.

تقييم الظهور النسيجي الكيميائي المناعي لفايروسات (EBV,HPV16 - 18,HSV1) في الحزاز المنبسط الفموي Assessment of The Immunohistochemical Expression of Epstein - Barr , Human Papilloma (18 ,16) And Herpes Simplex - 1 Viruses In Oral Lichen Planus

اسم المؤلف: تانیه عبد الاله الطحان
اسم المشرف: احلام حمید مجید
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: امراض ما حول الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد مرض الحزاز المسطح الفموي من الامراض الشائعة ولة مسار مرضي مرتبط بالخلايا المناعية ويرتبط باختلالات الاداء المناعي للجسم مع بعض الفرضيات التي تربط وقوع المرض بالعدوى الفايروسية. وھو من عائلة الھربس الفايروسية، بوقوع بعض الامراض ك "كثرة الوحيدات، EBV ار | oral lichen planus (OLP) is a common chronic inflammatory immune - mediated disease, The antigen responsible for inducing OLP is still unidentified, although viral agents have been proposed as etiologic factors. Viral infection (Epestin - barr,human papilloma and herpes simplex) has been hypothesized as a predisposing factor in the pathogenesis of this disease.Aims of study : The aim of this study was to assess the expression of Epstein Barr virus , Human Papilloma virus 16& 18 and Herpes Simplex virus - 1 immunohistochemistry in oral lichen planus cases and evaluate whether any clinical variant, histopathological or demographic feature correlates with the expression of these viruses.Materials and Methods : This study was performed on thirty formalin fixed, paraffin - embedded tissue Blocks of oral lichen planus retrospectively. An immunohistochemical staining done by using monoclonal antibodies EBV and HPV 18&16 and polyclonal antibody HSV1. Results : Expression of EBV was highly detected in epithelium of oral lichen planus cases (46.6%), no statistically significant correlation was found with clinical parameters.Astatistically significant with sex was observed.Immunostaning results revealed negative expression for both HPV16 & HPV18 in the studied cases except one case which was positive for each viruses. No statistically correlation was found with clinical parameters. Herpes simplex virus - 1 expression was positive in 12 cases (40%) of oral lichen planus cases. Statistically barely significant correlation with sex and clinical types, and a non significant one wasfound with age.ConclusionEpstein Barr and Herpes simplex viruses are present in considerable amounts in oral lichen planus whereas Human papilloma viruses 16 and 18 are rarely present.Taking into account the potential of viruses in OLP.

المؤشر الورمي CA153 عناصر مختارة في اللعاب وعلاقتها بصحة الفم والاسنان ضمن مجموعة من النساء العراقيات المصابات بسرطان الثدي Salivary Tumor Marker CA15 - 3 And Selected Elements In Relation To Oral Health Status Among A Group of Iraqi Breast Cancer Women

اسم المؤلف: بسمة عبد الباري عزیز
اسم المشرف: احلام طه محمد
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Breast cancer is the commonest type of malignancy worldwide and in Iraq. It is a serious disease that affects the general health and cause systemic changes that affect the physical and chemical properties of saliva leading to adverse effects on oral health.Aims of the study : The aims of this study were to assess the oral health condition (including dental caries, oral cleanliness and periodontal health condition), to evaluate the changes in salivary flow rate and to assess the concentration of tumor marker CA15 - 3 and selected elements (calcium, phosphorus, zinc, copper, total proteins) in saliva and their relation to oral variables among breast cancer women in comparison to control group.Subjects, Materials and Methods : The total sample consisted of 60 women aged 35 - 45 years attending Al - Kadhemyia Teaching Hospital. Those comprised 30 women newly diagnosed with breast cancer before starting treatment and 30 women without clinical signs and symptoms of breast cancer as a control group.Diagnosis and recording o f dental caries was done by using DMFS indexaccording to the criteria of WHO (1987). Plaque index of Silness and Loe (1964) and calculus component of Ramfjod index for periodontal disease (1959) were applied to assess oral cleanliness. Periodontal disease was evaluated using the gingival index (Loe and Silness, 1963) and loss of attachment level (Ramfjord, 1959). Stimulated salivary samples were collected and salivary flow rate, salivary CA15 - 3, calcium, phosphorus, zinc, copper ions and total proteins were determined.Results : Results showed that caries experience (DMFS) was higher among the study group compared with the control group but the difference was statistically not significant. The mean values of plaque index, calculus index, gingival index and loss of attachment were higher among the study group than the control group with a highly significant difference (P<0.01).The salivary levels of CA15 - 3 in breast cancer patients were higher than that of the controls with a highly significant difference (P<0.01). All the correlations between salivary CA15 - 3 and dental caries and periodontal diseases were statistically not significant.The salivary flow rate was lower among the study group than control group with a highly significant difference (P <0.01). Higher concentrations of salivary phosphorus, copper and total proteins were recorded among study group compared to control with statistically highly significant differences concerning phosphorus and copper (P<0.01). On the other hand, salivary calcium and zinc were lower among study group compared to control group with statistically highly significant difference concerning zinc (P<0.01). All the correlations between the caries experience and salivary constituents in the study and control groups were weak and not significant except the correlation with total proteins in the control group which was significantly positive. Concerning the correlations of oral hygiene and periodontal disease with salivary constituents, the study showed that all the correlations were weak and not significant except the correlations between the total proteins with loss of attachment in study group and with dental plaque in control group which were significantly positive relations.Conclusions : This study showed that the breast cancer patients had poor oral hygiene and higher rates of periodontal diseases and dental caries, therefore special preventive programs need to be designed for this group of patients. Also the results of this study could support the concept that salivary oncentrations of CA15 - 3 might serve to be used in the detection of breast cancer and/or the po operative follow - up of patients under treatment for carcinoma of the breast

تاثير انواع مختلفة من المواد الملونة على الاستقرار اللوني للحاصرات التقويمية الخزفية المرتبطة مع انواع مختلفة من المواد اللاصقة الضوئية التصلب : دراسة مختبرية Effect of Different Staining Materials On Color Stability of Sapphire Brackets Bonded With Different Types of Light Cure Orthodontic Adhesives (An In Vitro Study)

اسم المؤلف: الاء فالح البو حسن
اسم المشرف: نضال حسين غايب
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: لقد ازدادت الحاجة الى استعمال الاجهزة التقويمية التجميلية في هذه الايام مما دفع مقومي الاسنان الى البدء باستخدام الاسلاك التجميلية والحاصرات التجميلية بالاضافة الى الاشرطة المطاطية التجميلية.الحاصرات التقويمية الشبيهة بلون الاسنان انتجت من انواع مختلفة م | The demand for better esthetic during orthodontic treatment has increased now a days, so orthodontists starting using esthetic arch wires, brackets and ligatures.Tooth colored brackets were introduced in different types of materials.Sapphire ceramic brackets are one type of esthetic brackets and their color stability remains the main concern for the clinicians and patients at the same time.The present study design to evaluate the effect of three different staining materials (black tea, pepsi and cigarette smoke) on the stainability of sapphire ceramic brackets bonded with three types of light cure orthodontic adhesives whichinclude : Resilience, Enlight and Transbond. The sample consisted of three hundred sixty sapphire brackets. The brackets were divided according to bonding materials into three groups each group consist of one hundred twenty brackets, then each subgroup further subdivided into four groups according to the material they were immersed (distilled water, black tea, Pepsi and cigarette smoke) with thirty brackets each, then each group with ten brackets further subdivided according to time interval of immersion in each media into three groups one day, seven days and fourteen days at 37°C in the incubator. A UV - Visible spectrophotometer (Shimadzu, UV - 1800) was used to perform a light absorption test.ANOVA and LSD tests were used to identify the significant effects of the staining materials at a significance level P ? 0.05.It was found that the immersion time gradually influenced the color stability of the adhesive materials with sapphire brackets with the highest activity observed at fourteen days interval. The brackets bonded with Resilience light cure adhesive are the most type affected by staining materials, then followed by the brackets bonded with Transbond and finally the brackets bonded with Enlight lightcure adhesive.For the staining materials it was found that the cigarette smoke is the mostpowerful staining material, followed by tea and finally pepsi.From the above result we can conclude that the type of adhesive must takein consideration when the esthetic brackets have been used.

دراسة المضاعفات الفموية الوجهية, مؤشرات عامل - الغلوبيولين المناعي اللعابي A , الانترليوكين 6 وعامل تنخر الورم الفا في لعاب مرضى بيتا ثلاسيميا الكبرى في محافظة ميسان Study of Orofacial Complications, Salivary IgA, Interleukin - 6 And Tumor Necrosis Factor - Alpha Markers In Saliva of Beta - Thalassemia Major Patients In Missan Governorate

اسم المؤلف: جمال محبس ديوان
اسم المشرف: زاهدة جاسم محمد
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الثلاسيميا نوع بيتا هو الاضطراب الاحاد اليين الاكثر شيوعا في الشرق الاوسط، والتي تتميز بشذوذ في تركيب سلاسل بيتا من خضاب الدم مما يؤد الى ظواهر متفاوتة تتراوح بين فقر الدم الشديد الى عدم وجود اعراض سريريا.اهداف الدراسة : تحديد مدى انتشار المضاعفات الفمو | Beta - thalassemia is the most common monogenic known disorder in the Middle East, characterized by anomalies in the synthesis of the beta chains of hemoglobin resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic Individuals. Several immunological defects can be found in patients with beta - thalassemia major patients, among which circulating cytokines levels, impairment of neurophil, macrophage phagocytic and killing functions, this impairment may be due to iron over load.Aims of study : Aims of this study was to evaluate the prevalence of Orofacial complications in beta - thalassemia major patients, salivary flow rate, salivary levels of salivary immunoglobulin A, interleukin - 6 and tumor necrosis factor alpha and to evaluate correlations between salivary parameters (salivary immunoglobulin A, interleukin - 6, tumor necrosis factor alpha and salivary flow rate) in beta thalassemia major patients.Methods : Sixty subjects were participated in this study, they were divided into two groups, patients group composed of 30 patients with major beta - thalassemia, age rang (5 - 23), and 30 healthy looking subjects of both sexes as a control group, age rang (5 - 25). Data was collected using a special formula for general, medical and Orofacial examination sheet. Whole non - stimulated saliva was collected, measured and analyzed by enzyme linked immmunosorbent assay.Results : Most patients were in the first and second decade of life (90%), 20 (60%) were males and 10 (40%) were females with an age rage (5 - 23 years). The prevalence of Orofacial complications in beta - thalassemia major patients increased with age.The prevalence of orofacial complications in beta - thalassemia major patients were color change of oral mucosa (73.3%), rodent face (66.7%), maxillaryprotrusion (66.7%), saddle nose (63.3%), deep bite (26.7%)), open bite (13.3%) and spacing (10%).Regarding the salivary flow rate there was a statistically significant decrease in mean of salivary flow rate in patients group as compared with control group (P= 0.013).Laboratory investigations for salivary concentration of interleukin - 6, tumor necrosis factor alpha and salivary immunoglobulin A revealed highly significant to significantly in mean of these parameters in beta thalassemia major patients as compared with control group, interleukin - 6 (p=0.001), tumor necrosis factor alpha (p= 0.01) and salivary immunoglobulin A (p= 0.05) at P value ? 0.05.Conclusions : Beta - thalassemia major patients have specific Orofacial complications and a particular salivary cytokines compared to normal individuals.

سمك العظم الشدقي في مناطق مختلفة من الفك السفلي واهميتة السريرية في تثبيت المسامير الحلزونية احادية القشرة باستخدام جهاز الاشعة المقطعية متعدد الشرائح Thickness of Buccal Bone At Various Sites of Themandible And Its Clinical Significance In Monocortical Screws Placementusing Multislice Computed Tomography

اسم المؤلف: حوراء نوري عطا لله
اسم المشرف: لمياء حامد النقيب
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: اشعة الفم والاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Open reduction and internal fixation (ORIF) using miniplates and screws is the treatment of choice for mandibular fractures.Using miniplates and screws along the ideal line of osteosynthesis provides sufficient support and stability to the bone fragments to allow immediate function. It is important to know both : the region where the bone providesafirm anchorage for the screw,andthe topography of thedental apices and inferior alveolar nerve to avoiddamaging them when inserting the screwThe aim of study : The purpose of this study is to determine the thickness of buccal cortical plate and that of buccal bone using computed tomography,at the parasymphysis and mandibular body, thereby determining the area that provide afirm anchorage and the maximum length of mono - cortical screws that can be safely placed in these regions without injuring the tooth roots orinferior alveolar nerve.Materials and Methods : The sample of the present study was a total of 110 Iraqi patients (77 males & 33 females) aged (18 - 35) years old who admitted to Computed Tomography scan unit in AL - SadrTeaching Hospital in Al - Najaf city to getComputed Tomographic examination of facial bones from November 2013 to May 2014. The conventional section of CT (axial) wasused to do the measurements and dental planning analysiswhich is a specific investigation protocol was also used.The thickness of buccal cortical plate and the thickness of buccal bone were measured at the level of root apex of (canine, first premolar, second premolar) and at the level of : root apex and inferior alveolar canal in (mesial and distal root of first and second molar). Results : The mean buccal bone thickness at canine area was (3.7 - 4.3 mm), in premolars area (3.6 - 4 mm), in molar area (at the level of apex) (5.6 - 6 mm), in molar area (at the level of inferior alveolar canal) (5.2 - 5.6 mm) for females and males respectively. The mean buccal cortical plate thickness at canine area was (1.4 - 1.6 mm), in premolars area (1.5 - 1.7 mm), in molar area (at the level of apex) (2.4 - 2.6 mm), in molar area (atthe level of inferior alveolar canal) (2.4 - 2.3 mm) for females and males respectively.There was no statistically significant age and gender difference in buccal cortical plate thickness and buccal bone thickness. Conclusion : Buccal cortical plate thickness and buccal bone thickness in various sites of the mandible could be measured precisely using Multislice Computed Tomography which can guide surgeons in selecting the proper screw length without causing injury to rootapex or inferior alveolar nerve.

تاثير الجنس،العمر وفقدان السن على ابعاد القناة الثاقبة والعظم الامامي للقناة : دراسة بالتصوير المقطعي Effect of Gender, Age And Tooth Loss On The Dimensions of Incisive Canal, And Buccal Bone Anterior To The Canal (Computed Tomography Study)

اسم المؤلف: رياحين غازي رشيد
اسم المشرف: احلام احمد فتاح
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: اشعة الفم والاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: القناة الثاقبة تركيب تشريحي لھا موقعا ھاما في الفك العلوي الامامي، دراسة خصائص ھذه القناة وتقييم علاقتھا مع العظم الامامي للقناة ضروري خلال زرع القواطع المركزية العلوية. الاشعة المقطعية لھذه المنطقة يمكن ان توفر ميزات دقيقة عن حدود وموقع القناة الثاقبة | The incisive canal is an anatomical structure with an important location in the anterior maxilla, analyzing this canal characteristics and evaluated its relation to the bone anterior to the canal is necessary during dental implantation of maxillary central incisors. CT scan image for this region can provide accurate features about the border, position of incisive canal and status of buccal bone anterior to this canal.Aim of the study : To study for the effect of gender, age and tooth loss in area of maxillary central incisors teeth on the dimensions of the incisive canal and buccal bone anterior to the canal using spiral computed tomography.Subjects, Materials and Methods : The sample consisted of prospective study for 156 subjects of both gender. The sample were divided into two groups, 120 dentate group (60 male and 60 female) with age ranging from (20 - 70) and 36 edentate group(with missing both maxillary central incisor) (18 male and 18 female) with age ranging from (50 - 70), which was further divided into two group according to the duration of maxillary central incisorsteeth loss, long duration (5+) years which consists of 26 subjects and short duration (<5 years) which consists of 10 subjects. All subjects attended to Baquba Teaching General Hospital in Diyala for Computed Tomographic scan investigation for different Maxillofacial diagnostic purposes from November/2013 to April/2014.Using sagittal section of computed tomography scan, the following measurementswere done : A - The diameters of incisive canal were measured at crestal, middle and apical level and the total mean value of canal diameters was calculated. B - The length of incisive canal.C - The distance of buccal bone anterior to incisive canal at crestal, middle and apical level and the total mean value of buccal bone distances was calculated. D - The length of buccal bone anterior to incisive canal from the apical measurement of buccal bone to the alveolar crest. The measuring unit was in millimeter in all measurements.Result : Gender had moderately strong effect on the dimensions of incisive canal and buccal bone anterior to this canal, the mean values begin generally higher for male as compared to female for both control and cases group. Absence of maxillary central incisors decreased incisive canal length and buccal bone dimensions with mean values begin higher in control group than that in cases group, the effect of teeth loss on these selected measurements seem to be strong ;however, canal diameter remain unchanged with dental status and there was no effect of teeth loss on this parameter.There was a moderately strong to strong effect of the duration of maxillary central incisors teeth loss on canal length and buccal bone dimensions with mean values being significantly lower in long duration (5+) years than that in short duration (<5 years) while it had weak effect on incisive canal diameter for both gender. The results showed that there was no significant linear correlation between age and all selected measurements in male and female for both control and cases group. Conclusion : CT scan is a valuable tool to evaluate the anatomic variation at the examined area in the current study, gender and dental status are important factors that can affect incisive canal characteristics and amount of bone anterior to the canal. Clinicians should perform careful planning using CT scans before performing dental implant surgeries in premaxillary region.

قياس كثافة العظم السنخي في الفك العلوي في سن 13 - 15 سنة باستخدام المفراس الحلزوني Measurment of Maxillary Alveolar Bone Density At 13 - 15 Years Age Using Spiral Omputerized Tomography

اسم المؤلف: ازهار عبد الامير فرج
اسم المشرف: فخري عبد علي الفتلاوي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Bone density is a major factor that affect mini implant primarily stability. No Iraqi studies had been evaluated bone density related to mini - implant placement for orthodontic anchorage at age 13 - 15 years.The present research aims to evaluate gender, side and site differences in the bone density at various orthodontic implant sites for the maxillary alveolar bone (cortical; buccal and palatal and cancellous).The sample of this study consisted of CT images for patients who were attending Al. Shaheed Ghazi Al - Hariri Hospital/the Computerized Tomography department from January 2014 until May 2014. Twenty nine individuals (16 males and 13 females) had subjected to clinical examination, then 64 - multislice computed tomography scan data were evaluated and bone density was measured in Hounsfield unit at 21 points (9 points for each side and 3 points between the right and left central incisors).The results obtained showed that there were no significant differences in bone density between males and females and between the left and right sides. There are no significant differences in bone density between the maxillary buccal cortical bone and the palatal cortical bone except at lateral incisor and canine point where the palatal side had higher bone density than buccal side. The mean bone density of the cancellous bone in the anterior part was higher than that in the posterior of the maxilla.These differences in bone density between and within regions of the maxilla must be considered when placing mini implants and may provide valuable information when selecting sites, size, angle and placement methods for mini implant in the dental arch.

حاله الاكتئاب وعلاقتها بحاله الفم والخصائص الفزيوكيميائيه لدى طلاب المدارس من فئه عمر 15 سنة في مدينة الصويره - حافظة واسط - جمهورية العراق Depression Status In Relation To Oral Health Condition And Salivary Physiochemical Characteristics Among 15 Years Old School Students In Al - Swera City - Wassit Governorate - Iraq

اسم المؤلف: هدى سلمان خیاله
اسم المشرف: بان صاحب ذیاب
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان الوقائي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Depression is a common mental disorder that presents with depressed mood, its affect oral health through its effect on salivary function. The aims of this study were to assess the relation of depression status on prevalence and severity of dental caries and gingival inflammation among students aged 15 years in relation to salivary physical (flow rate and viscosity) and chemical characteristics concentration of (total protein, zinc, copper, chromium and lithium).Materials and methods : The total sample involved 800 students (males and females) aged 15 years old that were selected randomly, the depression status was measured using Children Depression Inventory (CDI) index that divided the students with depression into four groups according to severity of depression (low or average grade, high average grade, elevated grade and very elevated grade). The diagnosis and recording of dental caries was made by using Decay, Missing, Filled surface index (DMFs), according to the criteria of Manji et al (1989), while gingival condition was evaluated by using the gingival index by L?e and Silness, (1964). Salivary samples were collected under standardized condition from 30 students with very elevated grade and 30 students with low average grade, and then analyzed for measuring salivary flow rate and viscosity, in addition to the estimation of salivary elements (total protein, zinc, copper, chromium and lithium).Results : The prevalence of depression was 100%. The DMFs was lower among students with high average grade than other grades of depression with nonsignificant difference. The severity of dental caries was higher among students with elevated grade concerning D1, D3 and in very elevated grade of depression concerning D2, D4 with highly significant among D4 (P = 0.01). The prevalence of gingivitis in present study was found to be 100%, mild gingivitis occurrence found higher among low or average grade than other grades, but moderate gingivitis was more among very elevated grade, while sever gingivitis was absent. Salivary analysis demonstrated that the salivary flow rate was non significantly higher among students with low or average grade of depression than very elevated, while the viscosity of saliva was not significantly higher among students with very elevated grade. The data analysis of salivary elements found that the total protein and copper was higher among students with very elevated grade while other elements show the opposite result with significant difference concerning copper and zinc among females in very elevated grade, while others with not significant.Conclusion : The results of the current research revealed that caries experience and gingival inflammation increase with depression that has an adverse effect on salivary physicochemical characteristics. There were changes in saliva variables between low or average grade and very elevated grade of depression.

تاثير متسلل التسوس ممزوج مع او بدون مواد لاصقة تقليدية على ختم المينا السليمة : دراسة مختبرية The Influence of Caries Infiltrant Combined With And Without Conventional Adhesives On Sealing of Sound Enamel (In Vitro Study)

اسم المؤلف: مروة بلاسم محمد الجيبةجي
اسم المشرف: ضياء جعفر الدباغ
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The formation of white spot lesions around fixed orthodontic attachments is a common complication during and following fixed orthodontic treatment, which hinder the results of a successfully completed case.The aim of the study was to assess the effectiveness of the Caries Infiltrant (ICON®) on prevention of caries on the smooth enamel surface when applied alone or combined with conventional adhesives. To achieve this objective, seventy eight human premolar enamel discs were randomly assigned to six groups (n=13). The discs were etched and treated with resins of different monomer content forming the following groups : (1)Untreated etched samples served as the negative control, (2) ICON® (DMG), (3) Adper™ SB 2 (3M ESPE), (4) Heliobond (Ivoclar Vivadent), (5) ICON®+ Adper™ SB 2 and (6) ICON®+ Heliobond. Specimens were subjected to demineralization by immersion in hydrochloric acid (pH 2.6) for 18 days. Calcium dissolution into the acid was assessed by photometric test via spectrophotometer at 24 hour intervals.The results revealed that, there was a highly significant difference between the sealed groups and the unsealed (untreated) one (p?0.00) indicating that the unsealed specimens showed the highest amount of Ca ion loss among all other groups. Additionally, there was no significant difference between untreated specimens and the ICON® sealed ones. While, Heliobond decreased the Ca ion loss significantly compared to the untreated specimens and Adper™ SB 2 performed significantly better than both ICON® and Heliobond. Furthermore, the combination of ICON® with either Adper™ SB 2 or Heliobond served as the best protective measures and maintained the protective effect during the whole experiment period.Therefore, within the limitations of this in vitro study, it could be concluded that the use of low - viscosity Caries Infiltrant prior to application of the tested conventional adhesives increases their protective effect against demineralization.

تقييم تاثير بلازما النيتروجين المتوهج لغرسة التيتانيوم التجاري على الترابط العظمي بالتحليل الميكانيكي والتحليل النسيجي Evaluation of The Effect of Glow Plasma Nitriding of Commercially Pure Titantium Dental Implant On Osseointigration Through Mechanical And Histomorphometric Analysis

اسم المؤلف: حسن جواد فرحان المسعودي
اسم المشرف: شذى سليم الامير
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: دبلوم عالي
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الاتجاه الجديد للغرسات هوايجاد المواد التي تسرع عملية بناء العظم في السطح البيني للعظم والغرسه وتحسين الاندماج العظمي من اجل توفير التحميل الفوري والمباشر بعد وضع الغرسات وتقليص فترة الانتظار التي تكون مزعجه وغير مريحه للمرضى.الاهداف.تقييم تاثير طلاء ال | Introduction : The new trend of implants is to find materials which accelerate bone formation in bone implant interface and improve osseointegration to provide immediate or early loading after placement in addition to eliminate the waiting period which is usually uncomfortable and disturbs patients. Aim of study : To evaluate the effect of plasma nitride treatment of screw shaped commercially pure titanium dental implant on bond strength at bone implant interface by torque removal test and histomorphometric analysis after 2 and 6 weeks in comparison to non treated one.Materials and methods : Commercial pure titanium plates and screws were plasma nitrided for 10h using glow plasma nitride apparatus. X - ray diffraction (XRD) analysis , scanning electron microscope examination were carried out on the nitride surfaces of the plates ,contact angle measurement (Wettability) was done by applying drop of saline and blood on the nitrided surface and compared to control. The femur of 10 white New Zealand rabbits were chosen as implantation sites. The femur of each rabbit received two screws, one plasma nitride treated and one non treated and a total of 40 screws were implanted. Torque removal test was performed(by digital torque meter) to measure bond strength between implant and bone, after 2 and 6 weeks healing periods. For each period of time 18 screws were tested for the torque required to remove the implant from the bone and 2 screws were kept for histological examination. Results : The results revealed that the mean removal torque values for the plasma nitride treated implants was significantly higher than the non treated implants and over the two periods of time. There was an increase in the bond strength (torque value) of bone - implant interface with time. The torque removal force for plasma nitride implants after 2 and 6 weeks was (30.22, 59.56 N.cm ,respectively) and for non treated implants after 2 and 6 weeks were ( 23.61,56.11N.cm, respectively) and new bone formation ratio for plasma nitride treated implants after 2 and 6 weeks(3.36, 4.72 , respectively) and for non treated after 2 and 6 weeks(1.56, 3.04, respectively). Conclusion : Plasma nitriding of Cp Ti screws significantly increased the torque removal value and new bone formation ratio at 2 and 6 weeks compared with the non treated implant.Dental implant, Osseointegration, Digital torque meter

مواصفات الاطباق وادراكه والحاجة والطلب على تقويم الاسنان لدى الطلاب البغداديين بعمر 13 سنة : دراسة مقارنة مسحية ميدانية Occlusal Features, Perception of Occlusion And Orthodontic Treatment Need And Demand Among 13 Years Aged Baghdadi Students (A Comparative Cross Sectional Epidemiological Study

اسم المؤلف: زينب محمد رضا الجلبي
اسم المشرف: فخري عبد علي الفتلاوي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The study was conducted to assess the distribution, prevalence, severity of malocclusion and orthodontic treatment need in Baghdad governorate in relation to gender and residency, and whether it has increased or not over 12 years by comparison with previous survey in Baghdad.A multi - stage stratified sampling technique was used in this investigation to make the sample a representative of target population. The sample consisted of 2700 (1349 males and 1351 females) intermediate school students aged 13 years representing 3% of the total target population.A questionnaire was used to determine the perception of occlusion and orthodontic treatment demand of the students and the assessment procedures for occlusal features by direct intraoral measurement using veriner and an instrument to measure the rotated and displaced teeth. In summary the followingresults were obtained : 1 - 7.7% of the sample had some type of orthodontic treatment or consultation.2 - 39.1% of the students answered that they have malaligned teeth, being significantly more in female than males.3 - of the students who stated that they have malaligned teeth, 26% reported that they had crowded teeth, 26.6% had spaced teeth, 22.4% had protruded teeth and 25.7% had rotated and displaced teeth.4 - Regarding the answers of the effect of mal - aligned teeth, 70.3% answered that it affects their appearance, 17.7% chewing, and 7.7% speech while 4.1% answered that mal - aligned teeth did not affect appearance, chewing or speech.5 - the most common reasons for not seeking orthodontic treatment that the student thought that treatment is fear of pain (48.8%) and not important or not possible (25.8%).6 - One or more missing teeth due to extraction or trauma were found in 4.9% of the sample, and the most common extracted teeth were the first molars (1.6% mandibular and 0.9% maxillary). One or more rotated teeth were found in 38.3% of the sample; one or more displacement teeth were found in 19.6% of the sample.7 - The maxillary anterior region showed the highest prevalence of 2mm or more spacing (15.2%) and the mandibular anterior region showed the highest prevalence of 2mm or more crowding (12.6%); a maxillary central diastema of 1mm or more was found in 18.1% of the sample with a mean of 0.306±0.015mm.8 - Angle’s class I occlusion was found in 78.29%of the sample, class II in 19.5% (17.2% division 1 and 2.3% division 2) and 2.3% had class malocclusion (1.6% postural and 0.7% true). The mean overjet of the sample was 3.31± 0.04 mm. The mean overbite was 2.99±0.03 mm,9 - Midline shift (?1mm) was found in 54.3% of the sample (28% to the right and 26.3% to the left side).Soft tissue impingement was found in 3.3% (3% palatally and 0.3% labially). Normal lip form was found in 86.6%, contracting lip form in 10.1% and lip trap in 3.3% of the sample.10 - The DAI scores registered in this study ranged between 13 and 65 with a mean of 23.234±0.12. 7.3% with treatment highly desirable, and 4.5% with treatment mandatory.Significant changes in the proportions of malocclusions as a whole in school children were found over the last decade; also increase the need for orthodontic treatment among adolescents in the Baghdad commune and low perception level of the Baghdadi children to their own occlusion and the demand on treatment was remarkably low. This data will be useful for public oral health service and emphasize the need for orthodontic treatment among adolescents in Baghdad and stressing on the role of oral health education to encourage the children to undergo orthodontic treatment.

تاثير اضافة فلوريد الصوديوم كمطهر على بعض خواص مادة طبعة الالجنيت Effect of Sodium Fluoride Addition As A Disinfectant On Some Properties of Alginate Impression Material

اسم المؤلف: علاء مهدي حسين
اسم المشرف: رغداء كريم جاسم
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: صناعة الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقدمة : تلوث طبعات الاسنان مع اللعاب والدم من تجويف الفم يحصل مباشرة في عيادات الاسنان ومختبرات صناعة الاسنان يجعل من طبعات الاسنان الملوثة فقرات يصعب التعامل معها من وجهة نظر انتشار الاخماج. اشارت التقارير السابقة الى امكانية الطبعات الملوثة بتلويث ال | Introduction : Contamination of dental impressions with saliva and blood from the oral cavity occurs readily in dental clinics, direct interaction between dental clinics and dental laboratories makes contaminated dental impressions difficult items to deal with it from the cross contamination point of view. Previous reports indicated that contaminated impressions can cross infect gypsum casts that were poured against them, however immersing or spraying commonly used alginate impression material with disinfectant may adversely affect the accuracy of dies or casts obtained. Objective : The objective was to evaluate the effect of (0.25%,0.5%,1%, 2%, 3%, 4% ) of (NaF) addition on the self - disinfection of alginate impression material and its effect on setting time, tear strength, dimensional change and accuracy of alginate impression materials and to compare this with CHX containing alginate material related to the same properties mentioned above.Materials and methods : A total No. of 800 specimens were prepared in this study, they were divided into five main groups according to the type of the tests used (tear strength test, setting time test, dimensional change test, dimensional accuracy test, and bacteriological tests (Streptococcus mutans and Candida albicans).Six concentrations of NaF from (0.25% to 4%) and 0.1% CHX gluconate were mixed with alginate impression material and compare it with control alginate without disinfectant. Results : The self - disinfecting impression material containing NaF showed a total kill of microorganisms immediately after impressions were made.Tear energies for all experimental impression materials were greater than those of the control products. There were no statistically significant differences between the dimensional change tests and also reproduction of detail test, that contained (NaF) and one that did not. With regard to setting time of the impressions, statistically significant reduction were seen between the control and experimental groups of alginate impression materials. The experimental material that did not contain (NaF) had a considerably longer setting time than all of the other materials tested.Conclusion : the use of (NaF) and CHX disinfection when taking alginate dental impression is a good measure in reduction of contamination and cross infection and have a minute effect on dimensional stability and is recommended as step in protecting dentist and dental laboratories teams.

التاثير السمي للضوء الازرق المرئي على البكتيريا المشعشعة المصاحبة للورم الفطري والبورفيروموناس اللثوية في المرضى المصابين بالتهاب اللثة المزمن : دراسة مختبرية Phototoxic Effect of Visible Blue Light On Aggregatibacter Actinomycetemcomitans And Porphyromonas Gingivalis In Patients With Chronic Periodontitis (An In - Vitro Study

اسم المؤلف: علي رعد عبد العزيز
اسم المشرف: مها شكري محمود وفاق محمود الوتار
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: امراض ما حول الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الخلفية : على الرغم من ان ازالة الصفائح الجرثومية (الجير) وكلس الاسنان ( الحصى السنية ) بالطرق الميكانيكية يمثل حجر الزاوية في علاج امراض اللثة، يبقى ذلك بحاجة الى علاج مساند اذا علمنا ان التنظيف الميكانيكي لا يستطيع الوصول الى كل السطوح والاماكن حول السن | Background : Although mechanical debridement of dental plaque and calculus represents the corner stone in periodontal treatment , still it needs an adjunctive therapy since mechanical cleaning cannot reach all the surfaces and sites all around the tooth and within periodontal defect or the bacteria that resides inside periodontal tissues. Chemotherapy been used as an adjunctive therapy alongside with mechanical debridement in different compositions and routes, i.e. : chlorhexidine mouth washes (local routes) , antibiotics (systemic route) , yet chemical treatment might not be sufficient or cannot be used as in cases of drug sensitivity , difficulty to maintain effective concentration at wanted sites and lack of patient dexterity and compliance.Hence, arises the need for an alternative adjunctive therapy, photodynamic therapy has been used in recent years, taking advantage of the toxic effect of light on periodontal pathogens (bacteria). Aims of the study : The purpose of this study was to determine the toxic effect of visible blue light emitted from dental curing led light device of wavelength ranges between 400 - 500nm and energy of 1000 - 1200 mw/cm on the viability of periodontal pathogens (Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis), alone once , and with combination of 0.2% chlorhexidine mouthwash in vitro.Materials and methods : A.actinomycetemcomitans and P. gingivalis, was obtained from periodontal pockets of patient suffering from periodontitis by removing the plaque carefully to be cultured under anaerobic conditions for 3 days in suitable culture media using anaerobic jar in the incubator, presence of the wanted micro - organism is confirmed using Gram stain and biochemical activity tests.The amount (quantity of bacteria) was determined by direct colony counting and using visual software aids (open CFU software program) when needed.Visible blue light (LED curing light) from a commercially available device the same used in light - cured fillings in dentistry was used to generate a beam of visible blue light with amount of energy 1000 - 1200 mW/cm2 of a wavelength ranges between 400 - 500nm. Samples were subjected to blue light for increasing time intervals and were subcultured after each exposure.Same procedure of light exposure was repeated on plates containing chlorhexidine mouth wash in holes created with pasture tube, a standardized amount of chlorhexidine (0.1mml) was administrated into the holes of each plate, and then the inhibition zone was measured repeatedly for each plate after 72 hours of anaerobic incubation.

تسوس الاسنان والاحتياجات العلاجية لطالبات الثانوية لعمر (16 - 18) سنة وعلاقتهما مع نظافة الفم، المستوى الدراسي للاهل والتحليل الغذائي في مدينة المسيب/ محافظة بابل/ العراق Dental Caries And Treatment Needs Among 16 - 18 Years Old High School Girls, In Relation To Oral Leanliness, Parent'S Education And Nutritional Status, In Al - Mussayb City/ Babylon Governorate/ Iraq

اسم المؤلف: زهراء محمد حسین
اسم المشرف: سلافة خالد السامرائي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان الوقائي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Dental caries is a quite prevalent disease in Iraqi community however, baseline data concerning this disease and related etiological and risk factor are limited, concerning in selected geographic area in Iraq.Aims of study : This survey was made to investigate the prevalence and severity of dental caries in Al - Mussayb city/ Babylon governorate, the co - relation of this disease was studied with nutrition status and dietary analysis, oral cleanliness and parent'seducation among (16 - 18) years old in high school girls.Materials and Methods : The survey was conducted among high school girls, with a total sample of 900. Diagnosis and recording of dental caries and treatment needs were done according to the criteria of WHO. Dental plaque was assessed by using Plaque Index following the criteria of Silness and Loe. Nutritional status was recorded according to Body Mass Index indicator by applying anthropometric measurement. Dietary analysis was according to Dietary Guidelines in primary health centers in Iraq. Parent's education was divided in to five levels according to modification to educational status of Kuppuswamy's scale.Results : The prevalence of dental caries was (70.5%). The DMFS mean value was (4.94 ±SE 0.161) and the median was equal to (4.00) for the total sample. The DMFS value increased with ages with statistically highly significant difference (P<0.01), The highest percentage of examined girls were in need of one surface filling (60.8%), followed by those in need of two or more surface filling (36.6%). Result showed a weak positive highly significant correlation between plaque index and DMFS (r=0.315, P<0.01). A statistically highly significant difference in DMFS was recorded by different level of parent's education as a lower value of DMFS was seen among girls with high level of parent's education. Results showed statistically highly significant differences in the value of DMFS between different grade of Body Mass Index indicator (P <0.01, df=3), for total sample (11.7%) was malnourished and (65.77%) of girls was with normal weight. Statistically highly significant weak negative correlation was seen between DMFS and different food groups (fruits, vegetable, meat, grain and diary). A weak positive highly significant correlation between DMFS and sweet consumption was seen (r=0.155, P<0.01).Conclusions : Girls in high schools in Al - Mussayb city/ Babylon Governorate demonstrated a high prevalence of dental caries. Nutritional status, dietary habits, oral cleanliness and parent's education were found to affect the severity of this disease, thusthere is a need for public and school preventive programs among those population

ابعاد البلعوم فيما يتعلق ببعض المتغيرات السنية والقحفية للصنف الهيكلي الاول والثاني : دراسة مقارنة بالاشعة القياسية الجانبية عند البالغين Nasopharyngeal Dimensions In Relation To Some Dento - Cranium Variables of Class I And II Skeletal Patterns (A Cephalometric Comparative Study In Adults)

اسم المؤلف: عمار عباس فضل
اسم المشرف: فخري عبد علي الفتلاوي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The size of the nasopharyngeal airway was believed to have an important role in the development of the dentofacial structure. Therefore, this study was done on 60 subjects (30 males and 30 females) at age range 18 - 25 years. Cephalometric radiograph has been taken to each subject and the measurements were recorded. The sample was divided into two groups, class I skeletal relationship (15 males and 15 females) and class II skeletal relationship (15 males and 15 females). Comparisons between the different study groups were undertaken and the following results were obtained.In class I skeletal relationship, all the nasopharyngeal liner measurements and all the dento - cranium linear measurements are significantly higher in males than females, except lower airway thickness (PNS - ad1) and upper airway thickness (PNS - ad2)showed no significant gender difference. While all the angular measurements showed no significant gender difference.In class II skeletal relationship, most of the nasopharyngeal liner measurements have no significant gender difference, while all the dento - cranium linear measurements are significantly higher in males than females. In comparison for class difference between class I and class II skeletal relations in total sample, all the nasopharyngeal linear measurements have no significant class difference, except lower airway thickness showed significant difference which were higher in class II than class I and upper airway thickness showed significant difference which were higher in class I than class In the whole sample of the study, positive correlation was found between lower airway thickness and upper airway thickness.

معدل انتشار وشدة نقص التمعدن الضرسي القاطعي والعلاقة مع العوامل المسببة بين اطفال المدارس بعمر 7 - 9 سنة في محافظة النجف Prevalence And Severity of Molar - Incisor Hypomineralisation In Relation To Its Etiological Factors Among School Children Age 7 - 9 Years In Al - Najaf Governorate

اسم المؤلف: ولاء عامر عوفي
اسم المشرف: بان علي صالح
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب اسنان الاطفال
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Molar - Incisor hypomineralisation (MIH) is one of the biggest challenges with great clinical interest for dental practice because MIH has a great impact on the oral health as consequently, on the quality of life of children. Currently, the etiology of MIH remains unclear and is thought to be acquired via multifactorial, systemic disturbances during amelogenesis. There is no previous study concerning school children aged 7 - 9 years in Al - Najaf govemorate in order to estimate the prevalence and severity of molar incisorhypomineralisation and the possible associated risk factors. Objective : To estimate the prevalence , severity and the possible associated etiological factors of molar - incisor hypomineralisation and also to study the correlation between body mass index and molar - incisor hypomineralisation.Patients and Methods : A cross sectional study was conducted at Al - Najaf Govemorate, data were collected during the period from January to the end of April 2014. study population included the primary school children aged, 7 - 9 years of both genders who attending the primary schools of Al - Najaf govemorate. Children having amelogenesis imperfecta, tetracycline staining or undergoing orthodontic treatment at the time of study, those with completely broken crowns of the first permanent molars, or those whose parent/guardians refusedto missed data or not get back the questionnaire were excluded from the study. Sample size was calculated according to the standard equation and a total of 600 children were enrolled. A structured self - administered validated arabic language questionnaire and an examination sheet was measured. Body weight and height were measured and the body mass index was calculated according to the standards. Dental material and supplies were used in examination, Prior to the clinical examination the participating children were given a toothbrush and fluoridated toothpaste to brush their teeth thoroughly under the supervision of the researcher. The demarcated hypomineralization was recorded according to the 10 point scoring system depended on the European academy of pediatric dentistry evaluation criteria. The severity was assessed according to the clinical evaluation of the examiner and the presence of opacities. Data of the studied group were entered and analysed by using the statistical package for social sciences (SPSS) version 21 and appropriate statistical tests were used to assess the differences and correlations between variables. Results : The response rate was 84. 7% and the highest was in the 9 - year - old children, the participants were 532 children, the prevalence of hypomineralisation defect was 22.9%. The prevalence of demarcated hypomineralisation was increased concomitantly with the age, and the 9 - yearold children were the more affected. The overall prevalence of molar - incisor hypomineralisation among boys was lower than girls; (17.3%) and 22.6%, respectively. Molar - incisor hypomineralisation was more frequent in normal body mass index children. The total number of teeth of the studied group was 1464. The number of affected teeth was 3 81 represented (26%) of the total number of teeth. The molar and incisors were most affected with White/creamy demarcated opacities with no posr eruptive enamel breakdown, (27.7% and 15.2%), respectively. The severely affected teeth were 33 teeth, represented 4.5% of the total number of teeth of the 122 children , severely affected molars were 25 (5.1 %) and the severely affected incisors were 8 (0.8%), (P=0.001), Obeseand overweight were more likely to have more severely affected teeth and the severity was also increased with the age of child. Conclusions and Suggestions : The prevalence of Molar Incisor Hypomineralisation among Iraqi schoolchildren of Al Najaf govemorate was 22.9%, it was more prevalent among girls, the 9 - year - old children , normal body weight children and urban residents. Molars were more affected with than incisors. It was more prevalent in maxillary than mandibular teeth. The severely affected teeth represented 4.5% of the total number of teeth of the 122 children. , molars were more severely affected than incisors. The more severely affected teeth reported inobese and underweight children. Further studies are suggested for further understanding of the etiological and risk factors of the disease.

الحالة الصحية للفم لاطفال الرياض وعلاقتها بالحالة الاقتصادية والاجتماعية في محافظة النجف الاشرف العراق Oral Health Status Among Kindergarten Children In Relation To Socioeconomic Status In Al - Najaf Governorate - Iraq

اسم المؤلف: سهى مهدي حميد شبر
اسم المشرف: وصال علي العبيدي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان الوقائي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

تقييم بعض مقاييس اللعاب المناعية وتركيز عنصر الحديد وعلاقتهما بحالة صحة الفم للاطفال المصابين بفقر دم البحر الابيض المتوسط النوع الكبير كربلاء/العراق Evaluation of Selected Salivary Immunological Parameters And Iron Ion Concentration In Relation To Oral Health Condition Among Children With Beta Thalassemia Major (Karbala’ / Iraq)

اسم المؤلف: شيرين سمير يوسف
اسم المشرف: بان علي صالح
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب اسنان الاطفال
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Beta Thalassemia Major is an inherited, genetic blood disorder which affect the bone marrow's ability to produce 2Themoglobin 2Tmolecules( the component of normal1T 1T2Tred blood cells1T2T 1Tthat transports oxygen to the body's cells), thered blood cells will have been short lifespan and 2Tdestroyed1T2T 1Tat a1T 1T2Tfaster rate2T. These patients are typically transfusion - dependent, and have significant complications, including iron overload, immune system disorders, and bacterial and/or viralinfections which can affect the oral health condition. Aims of the study : The aims of the present study were to assess the severity of dental caries, dental plaque, gingivitis, levels of selected salivary immunological components and iron concentration in saliva, among a group of boys and girls with beta thalassemia major in comparison with the control group.Materials and methods : The study involved 17 boys and 23 girls with beta thalassemia major, aged 8 - 9 years compared to 17 healthy boys and 23 healthy girls with the same age group. dmfs/ds and DMFS/DS indices (WHO, 1987), plaque index (Silness and L?e,1964), and gingival index (L?e and Silness, 1963) were applied, unstimulated salivary samples were collected for estimation the salivary immunological components(Secretory Immunoglobulin A, lysozyme, peroxidase, and lactoferrin) in which they were analyzed by enzyme - linked immune sorbent assay kits and iron ion concentration in saliva which was analyzed biochemically.Results : Results showed that, caries - experience was higher among beta thalassemia children compared to healthy children. In primary teeth, a higher dmfs/ds mean value was recorded for study group (dmfs/ds=8.93±6.40) compared to control group (dmfs/ds=6.63±5.38), the difference was statistically not significant (p>0.05), except for girls the difference was statistically significant (p<0.05). For permanent teeth, DMFS/DS mean value was found to be higher instudy group (DMFS/DS=0.63±1.23) compared to control group (DMFS/DS=0.58±0.75), and the difference was statistically not significant (p>0.05). plaque index mean value was higher in study group (PI=1.69±0.45) compared to control group (PI=1.52±0.28), and the difference was statistically significant (p<0.05). gingival index mean value was higher in study group (GI=1.38±0.20) compared to control group (GI=1.22±0.10), and the difference was statistically highly significant (p<0.01). Correlation between dental plaque and gingivitis in study group was positive with statistically highly significant(p<0.01). There were some variation in the levels of salivary immunological parameters between study and control groups, the differences were statistically not significant(p>0.05), except that for peroxidase, the levels were higher in study group compared to control group and the difference was statistically significant (p<0.05). Iron ion concentrations were higher in study group compared to control group, the difference was statistically not significant (p>0.05). The correlation between secretory immunoglobulin A levels and dmfs/ds in study group was statistically significant (r= - 0.32, p=0.02). Lysozyme was correlated with DMFS/DS in study group and the correlation was statistically significant (r=0.38, p=0.01). lactoferrin was correlated with dmfs/ds and DMFS/DS in control group, and the correlation was statistically highly significant and significant respectively (r=0.52, p=0.00), (r=0.32, p=0.04). The correlations between iron ion concentrations and dental caries were statistically not significant(p>0.05) for both groups. All the correlations between measured salivary parameters and dental plaque and gingivitis were found to be statistically not significant(p>0.05) for both groups. The correlations between iron ion and immunological components were statistically not significant(p>0.05). The correlation between lysozyme and lactoferrin was statistically significant in both study group and control group respectively (r=0.36, p=0.02) and (r=0.36, p=0.01). The correlation between peroxidase and lactoferrin in study group was statistically highly significant (r= - 0.40, p=0.00). In control group, there was statistically highly significant correlation between secretory immunoglobulin A and lactoferrin (r=0.41, p=0.00). Conclusion : dental caries, dental plaque and gingivitis found to be higher in beta thalassemia major patients than normal children; they need periodic dental examination and effective preventive program.

تقييم تاثير التيجان المقاومة للفولاذ (تقنية الهول) على معالجة التسوس للطواحن اللبنية : دراسة رجعية Evaluation of The Influence of Preformed Metal Crown ((Hall Technique)) On The Managing of Carious Primary Molars A Retrospective Study

اسم المؤلف: ورف محسن موسى
اسم المشرف: بان علي صالح
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب اسنان الاطفال
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : When prevention of dental caries fails, and a child is exposed to the risk of pain and infection, the disease must be managed to reduce this risk. There is a growing evidence supporting more 'biological approaches' for managing dental cariesin primary teeth. The Hall Technique is one of the biological approaches for managing caries in primary molars which involves sealing caries beneath preformed metal crowns (stainless steel crowns). The crown is cemented over the tooth without theuse of local anesthesia, caries removal, or tooth preparation. The clinical steps for the Hall Technique are straightforward but, as with all dental care provision, appropriate treatment planning for the procedure requires skill. The Hall Technique offers anothermethod of managing early to moderately advanced, active carious lesions in primary molars, with good evidence of effectiveness and acceptability.Aims of the study : This study was conducted to assess the possible local factors that might happen along with using this novel technique regarding the possible adverse effect of the increased occlusal - vertical dimension and the condition of the surrounding tissues.Also to assess the correlation between the rate of the successful outcome with the caries extension towards the pulp.In addition to evaluate the cost - effectiveness of the Hall Technique in comparison with the conventional restorations. Finally to estimate whether this technique can be performed efficiently by both the specialized dentists and general practitioners in terms of putting the appropriate treatment plan based on the radiographic assessment.Material and methods : A retrospective research was conducted, standardized bitewing radiographs of 100 patients between 3 - 9 years old were evaluated for marginal bone changes. The distance between the cemento - enamel junctions and the alveolar bonecrest was measured. The dentine was evaluated as well regarding the existence of clear band of dentine and the extension of the caries towards the pulp of the tooth. - The cost effectiveness was conducted by creating a scenario of the possible outcomes for the treatment decision whether it was Hall preformed metal crown or traditional restoration. Incremental cost - effectiveness ratio was calculated to find the savingamount value. - A panel consisting of five general practitioners and three specialized dentists weas asked to evaluate twelve bitewings radiograph presented to them and give their opinion whether these radiographs are suitable for Hall Technique or not.Results : The result of this presented study showed that there is decreasing in the distance between the cemento - enamel junction and alveolar bone level at the Hall PMCs treatment side between the before and after insertion periods.it also showed thatthere's no different bone level changes between the Hall preformed metal crown treatment side and its contra - lateral side regardless of its condition (Hall preformed metal crowns, no Hall preformed metal crowns). - A high rate of successful treatment is highly associated with observing a clear band of dentine but it is not associated with the carious extinction toward the pulp as long as a clear band of dentine can be seen. - The Hall Technique is more cost - effective by making more cost saving and more gained teeth. The Hall Technique can be performed efficiently by both the general practitioners and the specialized dentist.Conclusion : according on the results of this research it can be settled that there are no adverse effects of using Hall preformed metal crowns regarding the increase of occlusalvertical dimension and the periodontal condition also the Hall Technique is more costeffective and can be performed by specialized dentists as well as the general practitioners.

تقييم تاثير وجود الكحول في الغسولات الفموية على تلاشي قوة السلاسل التقويمية المرنة المختلفة الاشكال : دراسة مختبرية Evaluation of The Effect of Alcohol Presence In Mouth Washes On Force Degradation of Different Configurations of Elastomeric Chains (An In Vitro Study)

اسم المؤلف: نور نوري عباس عبد الله
اسم المشرف: نضال حسين غايب
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Elastomeric chains are one of the most commonly used force delivery systems. They have the ability to exert an interrupted force, convenience of use, compatibility to oral environment and cost effectiveness despite of the inherited disadvantage which is force degradation.This in vitro study was designed to evaluate the effect of alcohol presence in mouthwashes on force degradation of different configurations of clear elastomeric chains from Ortho Technology company which are : closed, short and long under the effect of time at different intervals (Initial, 1day, 1, 2, 3 and 4 weeks) with exposure to different chemical solutions.A total of (540) elastomeric chains of three different types (long, short and closed) transparent in color, with an initial length (19mm) and about 50% extension (29mm) were used for the study. These elastomeric chains was divided into four groups and exposed to different chemical solutions (Distilled water, Listerine Original alcoholic mouthwash, Listerine Zero alcohol mouth wash, Ethanol 26.9%) twice daily for 60 seconds each according to manufacturer's instructions to measure the amount of force degradation in different time intervals. These elastomeric chains were incubated in covered glass containers at 37C? for the entire testing period.The results of the present study showed that the elastomeric chains were greatly affected by time, the majority of force loss occurred after the first 24hr. then followed by a less steep gradient for the rest of the working period.We can conclude that alcoholic mouth wash (Listerine Original) causes an increase in force degradation of all types of elastomeric chains while alcohol free mouth wash (Listerine Zero) causes less force degradation of all types of elastomeric chains, additionally closed configuration elastomeric chains has the least percentage of force degradation than other configurations of elastomeric chains.

الحركة الوحشية للطاحن العلوي الاول باستخدام انواع مختلفة من الاجهزة مسبقة الصنع : دراسة مختبرية مقارنة Distal Movement of Maxillary First Molar Using Different Types of Ready Made Appliances : An In Vitro Comparative Study

اسم المؤلف: ليث عمر المولى
اسم المشرف: هند طاهر جرجيس
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: تقويم الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Maxillary molar distalization is an important method for correcting class II molar relationship.The important thing in the use the distalizing appliances is to reduce the patient cooperation with minimizing the effects of distalizing appliances on the anchoring teeth.Aims : The aim of this study is to compare three different intra - maxillary molar distalization devices with different designs , force direction. Other aims are to compare the effect of these devices on first permanent molars in mesiodistal , buccopalatal, rotational aspects. It aims also to compare the effect of these devices on the first premolar ( which is a part of anchoring system ).Materials and Methods : The samples consisted of three groups; Frog appliance, K - Loop spring and Multidistalizing arch on typodont simulation system (Ormaco). Upper typodont arch of class II division I was used.Pre and postoperative digital images were taken and analysed using AutoCAD software for each group, Six parameters was measured and compared ; first molar ( vertical change ,tipping change ,rotation, distalization rate) and first premolar (vertical change ,tipping change).A significant value of 0.05 was determined.Results : The results of present study showed that the multidistalizing arch gave rise a significant distal tipping, extrusion, distopalatal rotation of first molar and gave rise the highest mean value of first molar distalization rate. Also, gave rise a significant distal tipping and extrusion of first premolar.Frog appliance showed the lowest mean value of molar distalization rate ,distal tipping and rotation , in comparison with Multidistalizing arch (MDA) and K - Loop spring. Also, these parameters ranged between the highest and lowest mean value in extrusion of first molar , first premolar vertical change , Also showed lowest mean of distal tipping of first premolar than Multidistalizing arch (MDA).K - Loop spring ranged between the highest and lowest mean value distalization rate , distal tipping of first molar. Also, showed the lowest mean value in extrusion of first molar and extrusion of first premolar and rotation of first molar.However, The K - Loop spring show mesial tipping of first premolar in comparisom with (MDA) and Frog appliance that show distal tipping of first premolar.Conclusions : It is concluded that the three methods are effective in molar distalization. The best method of first molar distalization ranged between the K - Loop spring and Frog appliance. Consequently the two methods showed good amount of molar distalization with minimal effects on first molar and first premolar.

تاثير التدخين الخفيف على المستويات اللعابية لانزيم الفوسفاتيز القلوي والاوستيوكالسين لدى مرضى التهاب اللثة المزمن Effects of Light Smoking On Salivary Levels of Alkaline Phosphatase And Osteocalcin In Chronic Periodontitis Patients

اسم المؤلف: لبابة عبد الصمد عبد الامير
اسم المشرف: باسمة غفوري علي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: امراض ما حول الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background Chronic periodontitis is an inflammatory disease that affects the supporting tissues of the teeth and it is common among adults. Smoking is an important risk factor for periodontitis that induces alveolar bone loss. Alkaline phosphatase enzyme is involved in the destruction of the human periodontium. It is produced by many cells such as polymorphonuclear leukocytes, osteoblasts, macrophages and fibroblasts within the area of the periodontium and gingival crevice. Osteocalcin is one of the most abundant matrix proteins found in bones and the only matrix protein synthesized exclusively there. Small osteocalcin fragments are found in areas of bone remodeling and are actually degradation products of the bone matrix.Aims of the study 1. Investigate whether light smoker chronic periodontitis patients exhibit different salivary concentrations of Alkaline Phosphatase and Osteocalcin compared to the non - smoker counterpart and compare to light smoker and nonsmoker control groups and 2.Correlate the clinical periodontal parameters (plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level) to the biochemical findings in light smokers and non - smokers chronic periodontitis and control groups. 3. Estimation of the salivary PH and flow rate and correlate them with clinical periodontal and biochemical parameters. Materials and Methods Five ml of unstimulated whole saliva samples and fullmouth clinical periodontal recordings (plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level) were obtained from study groups (25 light smokers and 33 non - smokers subjects, both with chronic periodontitis) and control groups (8 light smokers and 13 non - smokers subjects, both with healthy periodontium). All subjects were systemically healthy males, with age range (30 - 50) years. Salivary Alkaline phosphatase and Osteocalcin levels were determined by Colorimetric and Enzyme - linked Immunosorbent Assay, respectively. Salivary PH and flow rate were also measured.Results Smoker chronic periodontitis patients revealed non - significant differences in clinical periodontal parameters with non - smoker counterparts (P > 0.05) in terms of plaque index, probing pocket depth, clinical attachment loss, salivary PH and flow rate, with slight increase in plaque index value in smoker chronic periodontitis group(1.42±0.46) than non - smoker chronic periodontitis group (1.38±0.50), while there was slight decrease in mean value of probing pocket depth (3.90±1.78), clinical attachment level (3.22±0.93) and PH (7.58±0.50) in smoker chronic periodontitis group than non - smoker chronic periodontitis group (4.27±1.98), (3.74±0.85), (7.73±0.33) respectively. Flow rate value was higher in smoker chronic periodontitis (4.80±2.69) than non - smoker chronic periodontitis group (4.67±2.09). While there were highly significant differences in terms of gingival index and bleeding on probing (P ? 0.01). Osteocalcin levels were lower in smoker chronic periodontitis group (0.13±0.20) than non - smoker chronic periodontitis group (1.09±2.26) with significant difference (0.05 ? P > 0.01). Highly significant, strong, positive correlations were found between Osteocalcin concentration with plaque index and PH in non - smoker control group. Correlation analysis between Osteocalcin concentration and flow rate in smoker chronic periodontitis group revealed a significant, moderate, positive correlation.Mean of Alkaline phosphatase level was lower in smoker chronic periodontitis (11.14±4.53) than non - smoker chronic periodontitis (11.45±4.17) with a nonsignificant difference, while there was a significant difference in Alkaline phosphatase concentrations between smoker and non - smoker control subgroups.Correlation analysis between ALP concentration and clinical periodontal parameters (plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level) in chronic periodontitis smoker group appear non - significant, weak,negative and related non - significantly, weakly, positively with PH and flow rate. Clinical attachment level and PH correlated non - significantly, weakly, negatively with Alkaline phosphatase concentration, while plaque index, gingival index, bleeding on probing, probing pocket depth and flow rate related non - significantly, weakly, positively with Alkaline phosphatase concentration in chronic periodontitis non - smoker group. There was a significant, moderate, negative correlation between salivary PH and bleeding on probing and significant, weak, negative correlation regarding probing pocket depth in smoker chronic periodontitis group.A significant, weak, positive correlation between salivary flow rate and clinical attachment level was found in non - smoker chronic periodontitis group.There were non - significant differences between smoker chronic periodontitis and smoker control groups in terms of salivary PH, flow rate, Osteocalcin and Alkaline phosphatase concentrations. There were non - significant differences between non - smoker chronic periodontitis and non - smoker control groups in terms of PH, flow rate, Osteocalcin and Alkaline phosphatase concentrations. There were non - significant, weak increase in Alkaline phosphatase concentration compared to the increase in Osteocalcin concentration in smoker and non - smoker chronic periodontitis and smoker control groups, while there was a nonsignificant, weak decrease in Alkaline phosphatase concentration with increase in Osteocalcin concentration in non - smoker control group. Conclusion Within the limits of this study, it may be suggested that suppression of salivary Osteocalcin levels by smoking and weak increase in Alkaline phosphatase in smokers groups, may explain the deleterious effects of smoking on periodontal healthstatus.

تاثير انابيب الكاربون النانوية متعددة الجدران على الصلادة والتوصيلية الحرارية لمينا الاسنان Effect of Multi - Wall Carbon Nano Tubes On The Microhardness And Thermal Conductivity of Dental Namel

اسم المؤلف: محمد كاظم جواد
اسم المشرف: عباس فاضل علي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: الانسجة الفموية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The objectives of this study was to evaluate the effect of addition of Multi - Wall Carbon Nano ubes(MWCNTs) of different concentration (0.05 mg/mL - 1, 0.25 mg/mL - 1, 0.5 mg/mL - 1and 1 mg/mL - 1) with on either (dimethyl sulphoxide DMSO and distilled water DW) on tooth enamel. It is intended to evaluate enamel microhardness in (Kg.m - 2) and enamel thermal conductivity before and after the application of (MWCNTs).Ninety mandubular first premolar were prepared for this study. They were divided into two groups. The first group contains 54 sample to measure the microhardness of the enamel. To overcome the teeth curvature in microhardness measurement, the position of indenter were optimized to coincide linear buccal surface. The second group contains 36 sample to measure the thermal conductivity of the enamel were prepared by cutting enamel part in triangle form with a base of 7. 5 mm , height of 9. 5 mm and thickness of 2 mm according to the requirements of the Fourier’s law by abrasive disk.The results enamel microhardness showed that a significant increase in the enamel microhardness and enamel thermal conductivity for groups 0.05 mg/mL (group B) , 0.25 mg/mL (group C ) , 0.5 mg/mL (group D ) and 1 mg/mL (group E ) compared with control group (group A ) in DW media and DMSO media. The results showed a significant increase in the enamel microhardness and enamel thermal conductivity for polished samples compared with unpolished samples in DW media and DMSO media. DMSO media increases the enamel microhardness and enamel thermal conductivity more effectively than DW media. Scanning electron microscope showed a significant deposition of MWCNTs in the depth of the enamel exceeding the surface layer. The polished specimens had more deposited MWCNTs compared with non - polished specimens. However, the nano dimensions with conjunction with pre - chemical treatment enabled the MWCNTs to penetrate and settle down inside the enamel.The final conclusion highest mean value obtained was 1 mg/mL (group E ) in the enamel microhardness and enamel thermal conductivity suspension in DW media and DMSO media.

دراسة لتحويرات نانوية مختلفة لسطح زرعة الاسنان من معدن التيتانيوم النقي تجاريا باستعمال الطريقة الكيميائية وطريقة التبخير الحراري : تقييم ميكانيكي ونسيجي Study Different Nano Surface Modifications On CPTi Dental Implant Using Chemical And Thermal Evaporation Methods : Mechanical And Histological Evaluation

اسم المؤلف: بان ناهل شكر
اسم المشرف: رغداء كريم جاسم
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: صناعة الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Dental implants provide a unique treatment modality for the replacement of lost dentition. The clinical success of implants has been achieved not only because of the mechanical strength or cellent biocompatibility of the implant material but also owing to other characteristics such as surface properties.Aims of the study : The goal of this study was to consider the role of nanoscale topographic modification of commercial pure titanium dental implant using a thermal deposition and chemical etching methods for the purpose of improving osseointegration.Materials and methods : Commercial pure titanium rod was machined into 40 dental implants. Each implant was machined in diameter about 3mm, length of 8mm (5mm was threaded part and 3mm was flat part). Implants were prepared and divided into 4 groups according to the types of surface modification method used : 1st group (10 implant) machined surface i.e remained without nano surface modification (control),2nd group include (10 implant)etched with 15N H2SO4 and 30% H2O2,3rd group include (10 implant)were coated with nano CPTi powder by thermal evaporation deposition technique,4th group (10 implant)were chemically etched with H2SO4 and H2O2 method to obtain a nano feature then coated with nano powder of CPTi by thermal evaporation deposition technique.Nano surfaces were characterized by scanning electron microscope (SEM), Xray diffraction (XRD), atomic force microscope (AFM), thickness measurement and microscopical examination for the invitro experiments. For the invivo part of study, the tibia of 10 white New Zealand rabbits were chosen as implantation sites. The tibia of each rabbit received two screws. Biomechanical test was performed after two weeks healing periods. Implants from eight animals were tested for the torque required to remove the implantfrom the bone and the other two animals were prepared for histological examination.Results : For invitro results, scanning electron microscope showed that chemical etching of Ti substrate becomes highly porous and has surface consisting of nano_sized pits and the combination of chemical etching and thermal evaporation of nano Ti showed nano rod like structure. The results of removal torque means value after 2 weeks of implantation showed that, there was a gradual increase in the removal torque mean values in the studied groups as a follow (M±SD) : 12.625 N.cm ± 0.517 machined surface, 30.500 N.cm ± 4.071 chemically etched, 46.875 N.cm ± 5.938 nano CPTi coating by thermal evaporation method, 51.250 N.cm ± 5.175 combination method of chemical etching and nano CP Ti coating. In addition, the histological analysis showed improved quality of bone in response to the nano surface modified screws, especially the combination treated implants show a well developed mature bone characterized by a well developed bony threads and haversian canal. Conclusion : The biomechanical and biological properties of the bone - implant interface associated with the nano modified implants were improved comparing to the unmodified implants. All nano modified CP Ti seems to be well tolerated by the bone since no adverse tissue reaction was evident and they have better mechanical properties and excellent biocompatibility through the improved performance regarding the bone implant contact area than the untreated implants.

نقييم الالفا اميليز والكورتيزول في اللعاب كمؤشرين جيويين للتوتر النفسي وعلاقتها بامراض المفصل الصدغي بين عينة من طلاب كلية طب الاسنان Assessment of Alpha - Amylase And Cortisol As Salivary Psychological Stress Markers In Relation To Temporomandibular Disorders Among A Sample of Dental Students

اسم المؤلف: سرمد قيس علي
اسم المشرف: رجاء هادي الجبوري
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : University dental students perceived a higher level of stress prior to each examation especially the final. Therefore, there is a need for a useful noninvasive biomarker for measuring acute stress in those students. Many studies considered salivary alpha - amylase as a stress biomarker that is most often used in conjunction with cortisol. Understanding of the exact association between these two salivary stress markers are poorly established. Using a Helkimo anamnestic and clinical dysfunction scoring index for temporomandibular disorders can give a better insight about the association between these markers and temporomandibular joint disorders.The aims of study : was to evaluate the levels of salivary alpha - amylase and salivary cortisol in stressed students with temporomandibular joint disorders and the differences between these markers in relation to temporomandibular joint disorders students. This might give a better understanding to the role of psychological stress as an etiological factors for developing temporomandibular joint problems.Subjects, materials and methods : A total eighty (60 study and 20 control) participants aged between 20 and 24 years, were recruited for this study. The participants were final year undergraduate Baghdad university dental students who were examined and gave saliva samples before their final academic examination. Those with temporomandibular joint disorders problems were exposed to psychological stress and included on the study. While, those who were taking corticosteroids or hormone supplementation including oral contraceptives, or having history of head injury, orthodontic treatment, occlusal disharmonies and muscle tenderness due to systemic diseases as fibromyalgia, or having more than 2 missing posterior teeth were excluded. Salivary assay kits (alpha - amylase and cortisol) were used to measure those variables and a Helkimo anamnestic and clinical dysfunction scoring for temporomandibular joint disorders were utilized in this study.Results : There is statistically a highly significant difference of the salivary cortisol and salivary alpha - amylase in the patient group( stress with temporomandibular joint disorders) and control group. The absence of correlation between these salivary markers was founded. Correlation analysis of salivary cortisol with Helkimo anamnestic and clinical dysfunction score shows positive association expressed by significant probability of error (p) value while for salivary alpha - amylase there is no association.Conclusion : This study demonstrates that salivary alpha amylase can be used as a stress biomarker in conjunction with cortisol to assess temporomandibular joint problems due to psychological stress in university students.

الظواهر الوجهية والفموية، عوامل الاكسدة ومضادات الاكسدة في مصل ولعاب مرضى فقر الدم البحر الابيض المتوسط نوع بيتا في مدينة كربلاء Oro - Facial Manifestations, Oxidative Stress And Antioxidants Markers In Serum And Saliva of Patients With Beta Thalassemia Major In Karbala City

اسم المؤلف: مؤيد صاحب عباس شمسه
اسم المشرف: تغريد فاضل زيدان
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Thalassemia is a hereditary anemia resulting from defects in hemoglobin production. Beta Thalassemia, which is caused by a decrease in the production of beta globin chains, affects multiple organs and is ssociated with considerable morbidity and mortality. Aims of the study : The aims of this study were to study the oro - facial manifestations in beta thalassemia major patients and evaluation of the oxidative stress status in serum and saliva represented by malondialdehyde as xidative stress indicator and also assessment of serum and saliva antioxidants which are ceruloplasmin and uric acid in beta thalassemic patients with and without periodontitis and compared that with the healthy individuals in addition to investigate the relation between the clinical findings and laboratory investigations.Methods : Eighty seven (87) subjects were consented and contributed in this study, twenty eight (28) thalassemic patients with periodontitis, thirty (30) thalassemic patients without periodontitis and twenty nine (29) healthy subjects that were age matched with the patients. Oral examination has been done for each subject. Serum and saliva sample have been taken from each subject for analysis, to study malondialdehyde as oxidative stress and antioxidants ceruloplasmin and uric acid.Results : Malocclusion was the most common prevalent oro - facial manifestations (60%), followed by rodent face (35%), brown pigmentation of oral mucosa (23%) and incompetent lip (8%). The mean serum and saliva malondialdehyde were significantly higher in thalassemic patients with periodontitis than that in thalassemic patients without periodontitis and healthy subjects (p<0.001), while the mean serum and saliva ceruloplasmin were significantly higher in control group than that in other study groups (p<0.001). The mean serum uric acid was significantly higher in thalassemic patients without periodontitis than that in thalassemic patients with periodontitis and healthy subjects (p<0.001) and the mean saliva uric acid was significantly higher in thalassemic patients without periodontitis (p<0.05) compaired with thalassemic patients with periodontitis and healthy subjects. The results of this study showed that there were no significant correlation between serum and saliva malondialdehyde in any of study groups, while there was a positive significant linear correlation between serum and saliva ceruloplasmin in thalassemic patients with periodontitis (p<0.05), a negative significant linear correlation in healthy subjects (p<0.05) , a positive significant linear correlation in thalassemic patients without periodontitis (p<0.001). The results showed that there was a positive significant linear correlation between serum and saliva uric acid in thalassemic patients with periodontitis (p<0.05), a positive significant correlation in thalassemic patients without periodontitis (p<0.001) and no significant correlation between serum and saliva in healthy subjects. On the other hand there were no significant correlation between serum and saliva (malondialdehyde, ceruloplasmin and uric acid) and clinical attachment loss (p>0.05), except a positive significant linear correlation between clinical attachment loss and salivamalondialdehyde (p<0.05) was found. Conclusions : Malocclusion was the most prevalent oro - facial manifestations of beta thalassemia major patients. Oxidative stress play a significant role in the pathogenesis of beta thalassemia ,which was the main cause of red blood cells destruction represented by significant elevation of serum and saliva malondialdehyde and significant reduction of ceruloplasmin. Also oxidative stress remain contributing factor in periodontal tissue destruction, which was appeared as a significant increased level of saliva malondialdehyde and decreased saliva ceruloplasmin in patients with periodontitis and there was a significant correlation between saliva malondialdehyde and clinical attachment loss

التقييم النسيجي والكيمائي النسيجي المناعي لتاثير الاستخدام على VEGF/ N - acetyl D - glucosamine الموضعي ل شفاء العظم : دراسة تجريبية على الجرذان Histological And Immunohistochemical Evaluation of The Effect of Local Exogenous Application of VEGF /N - Acetyl D - Glucosamine On Bone Healing : (Experimental Study In Rats)

اسم المؤلف: ایناس فاضل كاظم
اسم المشرف: عذراء يحيى الحجازي
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: الانسجة الفموية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Bones are rigid organs that constitute part of the endoskeleton of vertebrates. They support and protect the various organs of the body, produce red and white blood cells and store minerals. Bone tissue has been shown to contain numerous cell - to - cell signaling peptides called growth factors. These growth factors are thought to have important regulating effects for bone remodeling and bone healing. Several growth factors are expressed during fracture repair. of these, vascular endothelial growth factor (VEGF), is of particular interest because of its ability to induce neovascularization (angiogenesis). The responses to exogenous VEGF observed that a slow - release formulation of VEGF, applied locally at the site of bone damage, may prove to be an effective therapy to promote human bone repair. N - acetyl glucosamine is a monosaccharide, which are the derivatives of carbohydrates, it helps the body in making several other chemical agents which are used in the formation of bones, muscles and cartilages.N - acetylglucosamine is very effective in providing support for the skeletal system of the humans, and may provide strength to the ligaments and joints, and the skin remains fresh and glowing. Aims of study1. To identify role of local exogenous application of vascular endothelial growth factor and application of N - acetyl - D glucose amine as a biomaterial in bone healing.2. To analyze the expression of Vascular endothelial growth factor, tissue non specific ALP and bone matrix protein (collagen - 1) by immunohistochemistry in created bone defect after application with different biomaterials in a rat model. Materials and MethodsIn this experimental study fifty four male Albino rats, weighting (300 - 400) gram, aged (6 - 8) months were used and maintained under control conditions of temperature, drinking and food consumption. The animals were subjected for a surgical operation of medial sides of both tibial bone ( right side was considered as experimental site ,while left be the control one). The animals were divided into following groups according to the applicable growth factors.A. Contol group the bone defect treated with 1?m of normal saline and its number represented the all number of the following experimental groups as the left side of each animal considered to be the control.B. Experimental group includes ? Group I contains (18) rats, the bone defect treated with 1?ml of Vascular endothelial growth factor. ? Group II contains (18) rats the bone defect treated with 1?ml of Nacetyl D - glucosamine.? Group III contains (18) rats, the bone defect treated with 1?m combination of (VEGF& N - acetyl D - glucosamine).Every single group composed of 18 rats that studied in three periods 3,7,10 days (6 rats for each period). Histologic and immunohistochemical evaluation for VEGF, alkaline phosphatase and collagen type 1 were carried for all animals.Results : A. For Histological findings An early apposition of osteoid tissue was detected in VEGF group. Osteoblast, osteocyte and osteoclast cells count were demonstrated to be with high mean value in VEGF group followed by N - acetyl Dglucosamine group in periods 3,7,10 days post operative of bone defect.Inflammatory phase as first step in bone healing was detected in all study groups at 3 day, and combination group records highest value in the mean of inflammatory cell count in comparisum to others. Finally thestudy demonstrated that exogenous VEGF/ N - acetyl D - glucosamine has a direct effect on osteoblastic activity.B. For Immunohistochemical findings The present result showed positive expression of VEGF by bone marrow stromal cells, adipocytes, mesenchymal stem cells, precursor endothelial cells, and bone cells include osteoblasts and active osteocytes in different periods in all groups but in different score. Therefore, our primarily data provide evidence that VEGF activity is essential for appropriate bone formation and mineralization in response to injury. At day 3, bone marrow stromal cells in combination group records highVEGF expression, VEGF group is the second. At 7 and 10 day VEGF group records a highest VEGF expression in comparisum to other groups. The data showed a high expression for ALP during the formative stages of bone in all groups except combination group shows the lowest count for positive cells that expressed ALP.the present results showed that at day 3, bone marrow stromal cells in VEGF and N - acetyl D - glucosamine group records high collagen type I expression. Conclusion : the study demonstrated that low application of VEGF / Nacetyl D - glucosamine could be an effective therapeutic for bone injuries, these data are promising for a possible future clinical usage.

Assessment of The Oral Findings, Salivary Oxidative Status And Iga Level Among Group of Workers Exposed To Petroleum Pollutants In Al - Daura Oil Refinery

اسم المؤلف: امير سعد حمزة
اسم المشرف: Jamal Noori Ahmed
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: طب الاسنان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Petroleum is perhaps the most important substance consumed in modern society. Petroleum is unique and s a complex mixture of thousands of compounds. Oil refinery workers are continuously exposed to numerous hazardous materials and working conditions that place them at continuous risk of injury and death.Petroleum contains the heavy metals as a natural constituent or as additives. The main threats to human health from heavy metals are associated with exposure to lead, cadmium, mercury and arsenic. Several carcinogenic metals such as arsenic, cobalt, chromium, lead, mercury, and nickel induce redox reactions in living systems. These metals induce the production of reactive oxygen species in both in vivo and in vitro systems. These radicals have rendered oxidative damage to deoxyribonucleic acid, proteins, and lipids. Secretory immunoglobulin A is the main immunoglobulin found in mucous secretions from the tear glands, salivary glands, mammary glands, the respiratory system, the genito - urinary tract, and the gastrointestinal tract.Aims of the study Assessment of the salivary levels of heavy metals among the workers of Al - Daura oil refinery, assessment of the oxidative status in the oral cavity of the workers by measurement of Malondialdehyde and Superoxide dismutase markers,assessment of the oral immunological activity by measurement of secretory immunoglobulin A level in saliva and Assessment of the oral findings among the workers.Subjects, Materials and Methods This study was done in Al - Daura oil refinery, samples consist of 60 workers involved in refining processes as study group subdivided into three subgroups which represent the different sections in the refinery and 20 subjects not involved in refining processes as control group. Oral examination and saliva collection was done to assess the oral findings and measure the level of heavy metals (lead and cadmium), oxidative status (Malondialdehyde and Superoxide dismutase) and secretory IgA.Results The mean of salivary lead and cadmium was higher in study group (6.34 ?g/dl and 0.56 ?g/l respectively) than that of control group (3.3?g/dl and 0.34 ?g/l respectively) with highly significant difference (p

اثر عوامل البيئة الاجتماعية على سلوك سيطرة وزن المراهقين في المدارس الثانوية في مدينة بغداد Impact of Socio - Environmental Factors Upon Adolescents' Weight Control Behaviors In Secondary Schools At Baghdad City

اسم المؤلف: بثينة بشير صالح
اسم المشرف: اقبال غانم علي معلة
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: التمريض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: دراسة وصفية(مقطعية) اجريت في جانبي الكرخ والرصافة لمعرفة اثر عوامل البيئة الاجتماعية على سلوك سيطرة وزن المراهقين في المدارس الثانوية في مدينة بغداد للفترة من 20 من شهر نيسان لعام 2013 الى نهاية تشرين الاول لعام 2014.تهدف هذه الدراسة للتعرف على عوامل ال | Overweight and obesity are one of the biggest threats for public health, both in children and adolescents. Fair enough, a lot of attention is paid towards this threat. However, hardly any attention is paid to weight loss behavior in normal weight children and adolescents. Teenagers who consider themselves as too fat who were negative body image a high risk for unnecessary weight loss behavior. A Cross - Sectional Descriptive study was conducted on impact of socio - environmental factors upon adolescents' weight control behaviors in secondary schools at both sides Al - Karkh and Al - Risafa sectors of Baghdad city. The study period started from 20th of April 2013 to the end of October 2014. The study aimed to find out the relationship between weight control, and socio - environmental factors and adolescents' demographic data (age, gender, Body Mass Index, and socio - economic status), and find out the impact of socio - environmental factors upon adolescents' weight control behaviors. A non - probability (purposive sample) of secondary adolescent schools , while 1254 adolescents' students were chosen randomly from that schools. The study instruments consist of four parts. The first part concerned with adolescents' students and their parents? socio - demographic data; the second part is about adolescents' students eating patterns and their meals list at home. The second part is divided into six sections. The 1 section consists of 28 items : the adolescents' students eating patterns and meals list at home, section 2 and 3 contains 16 items : for eating the snacks and fast food, section 4 is composed of 12 items : which are about adolescents' students eating time in schools with peers, section 5 has 12 items : related to the reasons of skipping meals, and section 6 consists of 6 items : about adolescents' lifestyle. The third part is about the physical activities and dieting programs, it consists of 7 items. The fourth part 10 items are related to the adolescents' weight control behaviors. Test - retest determined the reliability of the study instrument which was r = 0.90. A descriptive statistical analysis (frequencies and percentages) and inferential statistical analysis, and (chi - square) were used. The result of the study sample indicated that more than two fourth of the Body Mass Index was almost equal in under or normal weight while the another half were overweight and obese, and the sample's family socio - economic status was in high or low level status. The study indicated most of the adolescents' took breakfast at home, eating snacks, reasons of skipping breakfast meal, and their lifestyle had a highly significant association with their gender and that indicate no differentiate between their gender eat what they want at any time. The results of the study reflected the adolescents' students eating lunch and dinner at home, and reasons of skipping breakfast meal had no significant association with their ages. The adolescents' students favorite to eat snacks and fast food at home and it's had a highly significant association with their Body Mass Index. Also there is a highly significant associated for the study sample socio - environmental factors with their socio - economic status. The results of the study reflect that is a highly significant association between eating habits and their total weight control behavior methods. The study recommended the Ministry of Health need to activate the nutritional program strategies for weight management within school health services, and need to increase establishment other especial centers for managing adolescents from risk for overweight and obesity like as Obesity Research and Therapy Unit in AL - Kindy College of Medicine and Nutrition Research Institute. I suggest a schedule as a guideline for healthy nutritious for weight control and achieve daily physical activity Finally Ministry of Education need to involved teachers in that strategies and training them in relation to these programs.

تاثير التقنيات المساعدة على الانجاب على نتاج الحمل في مستشفيات بغداد : دراسة مقارنة Impact of Assisted Reproductive Technology On Pregnancy Outcome In Baghdad Hospitals : Comparative Study

اسم المؤلف: رغد زغير دوسر
اسم المشرف: عز الدين فخر الدين بهاء الدين
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: طب الاطفال
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الخلفية : في عام1978 ولادة الطفل الاول في المملكة المتحدة من قبل تقنيات المساعدة على الانجاب، ازداد كلا من التقنيات المتطورة التي تستخدم لعلاج العقم وعدد مراكز الاخصاب التي توفر خدمات التقنيات المساعدة على الانجاب بشكل كبير في كثير من البلدان,وتشمل التقنيا | Background : In 1978 the first baby born in the U.K by the techniques of assisted reproduction. both the use of advanced technologies to overcome infertility and the number of fertility clinics providing ART services have increased steadily in many countries. ART includes fertility treatments in which both eggs and sperm are handled in the laboratory (in vitro fertilization (IVF), intra cytoplasmic sperm injection (ICSI), gamete intrafallopian transfer (GIFT), zygote intrafalopian transfer(ZIFT), and related procedures). Women who undergo ART procedures are more likely to deliver multiple - birth infants than those who conceive naturally because more than one embryo might be transferred during a procedure. Multiple births pose substantial risks to both mothers and infants, including pregnancy complications like, preterm delivery, and low birth weight infants. Caesarean section, pregnancy induce hypertension, gestational diabetes, congenital malformation.Objective : To assess the impact of assisted reproductive technology on pregnancy outcome and compare them to those of natural conception. Methodology : The present study employs a descriptive - purposive design carried out for pregnant women after assisted reproductive technology attending labor wards of Baghdad Hospitals, Kamal AL - samarae Hospital, Baghdad Teaching Hospital, AL Imamain AL - Kadhmien Teaching Hospital, Private Nursing House Hospital, AL - Elwya Maternity Teaching Hospital, AL Imam Ali Hospital, AL - Karkh Maternity Hospital for, and Ibn AL Baladi Hospital. The period for collecting the data was four months (1st February 2014 to the 1st Jun 2014). The study sample divided into two groups : 100 women conceived after assisted reproductive technology(study group), 100 women conceived naturally (control group). Validity through a panel of (15) experts and the reliability of the questionnaire is determined through the pilot study. For the purpose of the present study a questionnaire format was constructed. The data has been managed and analyzed by using descriptive and inferential statistics. Results : An increased pregnancy complication as well as higher risk of obstetrical, perinatal, and neonatal complications was observed in ART compared with natural pregnancy included in this study. The women conceived by ART were older than those conceived naturally (30.86_+ 4.76 vs 25.85 - +7.78) ART pregnancies were associated with a higher risk of early pregnancy bleeding (8% vs 42%), antepartum heamorrhage (4% vs 13%), placenta previa (6% vs 15%), pregnancy - induced hypertension (29% vs 46%), gestational diabetes (7% vs 20%), preterm rupture of membrane (11% vs 25%), preeclampsia (3% vs 12%), obesity during pregnancy (47% vs 65% ), preterm delivery (16% vs 48%), cesarean section( 67% vs 100%), low birth weight (12% vs 32.5%), multiple birth (twin 11% and twin tri 7% vs.0.0%), the period of the newborn child stay in the neonatal intensive care unit (15 days 47.8% vs. 0.0%)Conclusion : The pregnancies after ART are associated with higher risk of multiple pregnancies, obstetrical, perinatal, as well as neonatal complications in comparison with natural pregnancy. Recommendations : Educate mothers who become pregnant using the techniques of assisted reproduction importance they received of their children examined periodically, with long - term developmental follow - up on a regular basis, to exclude late complication of the pregnancy outcome. Emphasis should be on the birth of healthy infants primarily using Embryo single transfer (EST),to reduce the incidence of low birth weight and preterm delivery.

تقويم التعلم الموجة الذاتي للممارسات المختبرية المتعلقة باعطاء الدواء لطلبة كليات التمريض في جنوب العراق Evaluation of Students' Self - Directed Learning Laboratory Practices Related To Administration of Medication In Southern of Iraq Nursing Colleges

اسم المؤلف: حيدر علي حسين الوحيلي
اسم المشرف: نرمين بدري توفيق البارودي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: التمريض
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقدمة : الصعوبات التي تواجه تعليم التمريض تكمن في انتاج القوى العاملة التمريضية وهذا يتطلب رعاية الطلاب بالكفاءات اللازمة التي من شانها تسهيل انتقالهم من طلبة التمريض الى ممرضين كفوئين مؤهلين في وظيفتهم. حيث ان للتعلم الموجه الذاتي اهمية كبرى في تطوير ق | Background : The challenge in nursing education lies in the production of a nursing workforce. This requires nurturing the students with the necessary competencies that will facilitate their transition from nursing students to qualified staff nurses. Self - directed learning is of great importance to the professional development of nursing students. It enables them to expand the knowledge and enhance the quality of their practice. Nursing students must keep abreast of new information, current and emerging trends, medical technology and related scientific and professional publications to be able to function effectively in a constantly changing workplace. Self - directed learning helps nursing students remain flexible, open to change, current in practice skills, and at the same time it helps in the growth of the students’ confidence and professionalism. Objectives : The aim of the study is to evaluate the students' self - directed learning laboratory practices related to administration of medications procedures (Intramuscular injection, Intravenous injection and oral medication ). Methodology : Across sectional design was has been carried out at three colleges of nursing in the south of Iraq and used in the present study from 23th February 2015 to 15th May , 2015. To evaluate nursing students' self - directed learning in applying laboratory practices related to administration of medication in southern of Iraq. The present study was conducted in southern Iraq at three nursing colleges which included (Missan, Thi - Qar and Basra nursing college ). Purposive sample was selected which consist of (90) first year of nursing students( 45) male and (45) female and the sample contained (30) students from each college after the consent of the participants. The check - list consists of two parts, the first part is general information about the study sample of nursing students (4 items) and the second part is an ideal observation check list which includes (73) items including three procedure technique which consists of the intramuscular injection ( 25 )items, intravenous injection (28) items and oral medications (20) items that concerned with students self - directed learning laboratory practices technique. Each procedure has three steps of strategy technique (preparation , equipments and procedure ) which are considered important steps that must be performed by students. The items concerning students self - directed learning laboratory practices are rated on three levels likert scale; (satisfy , need more practice and unsatisfied ). Reliability of the evaluation check - list competency is determined through the use of check - list is based on Cronbach's practices. The instrument validity is determined through a panel of (15) experts.The present study are analyzed through the application of two statistical approaches. A descriptive statistical approach includes (frequency, percentage, Mean of score (M.S.) and standard deviation (SD) and an Inferential statistical approach includes (Chi - Square,t - test, ANOVA). Results : The result of the study indicate that the majority of the first nursing students' satisfaction in general have high level of the self - directed learning in laboratory practices. During application the result show that s (96.7%) intravenous , (93.3%) intramuscular and (88.9%) oral medications administration and also the study declared non significant relationship between self - directed - learning (students lab practice) and their demographic data. Recommendations : This study recommended that : . 1 - Introduce a policy and supportive plans to assist and encourage personnel of every nursing students to the colleges.2 - Extensively apply self - directed learning as a method of learning for their professional and self - development and develop visual aids for learning and set up comprehensive.3 - Update the sources of learning for example public library , the internet service, communications room and/or educational technology room. 4 - Facilitate suitable environment for nursing students in searching for information they need for learning and self - development. 5 - Every these recommendation addressed to ministry of higher education/ colleges of nursing.

تقييم رضا المسنين باتجاه الرعاية الصحية في دار المسنين في مدينة بغداد Assessment of Elderly'S Satisfaction Toward Health Care At Geriatric Home In Baghdad City

اسم المؤلف: عادل عبد الزهرة عطية
اسم المشرف: محمد فاضل خليفة
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: التمريض
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت دراسة وصفية لتقييم رضا المسنين باتجاه الرعاية الصحية في دار المسنين في مدينة بغداد للفترة من 26 تشرين الثاني 2013 الى3 ايلول 2014، تهدف الدراسة الى تقييم رضا المسنين باتجاه الرعاية الصحية من خلال مجالات الرعاية مثل (طلب الرعاية الصحية، تناول الع | A descriptive study concerning the assessment of elderly's satisfaction toward health care at geriatric home in Baghdad city. From November 26th, 2013 to September 3rd, 2014. is to assess the elderly's satisfaction toward health care through the use of the domains of health care seeking, nutrient intake, physical activity, drug use, psychological aspect, social aspect, and to find out the relationship between elderly's satisfaction and their demographic characteristics of age, gender, marital status, level of education, health state, and economic status. The selection of the sample through a non - probability 'purposive sample' is (N=86) from elderly aged 65 years and over who reside in geriatric home. Data are collected through interview using questionnaire tool and analyzed through the application of descriptive and inferential statistical analysis which reveal, the reliability of the questionnaire which is determined through a pilot study and the validity is achieved through a panel of (18) experts. The study results showed demographic characteristics analysis of the sample concerned, the large number of the sample that was with age (65 - 69) years (34.9%) and also showed that majority of study sample (59.3%) are men and the remaining are women, and with regard to marital status it was found that many of them are widows and their percentage (38.4%) Regarding subject level of education, the greater number of them are illiterate and they are accounted for (48.8%) In addition, the majority of the elderly who suffer from chronic disease are (73.3) while the majority of them have no previous surgery and their percentage (58.1%) Regarding housing ownership (74.4%) they are absent or (do not have a house), and most of the elderly's monthly income is (53.5%) insufficient. The present study concluds : the vast majority of the elderly population are illiterate who do not have opportunity for better education. In addition, the elderly were generally satisfied with health care, and it was the best domain of elderly satisfaction is the nutrient intake and the less domain of health care seeking. While the study showed that, the demographic characteristics of elderly had no impact upon their overall satisfaction toward health care except the level of education. Therefore, that gender affects the satisfaction of the elderly in seeking of health care. In addition, the monthly income effects on elderly satisfaction with nutrient intake. As well as the age and suffering from chronic diseases affecting the physical activity domain and with level of elderly satisfaction in this aspect there is an impact of each gender, level of education and monthly income of the elderly on their satisfaction towards the social domain, the age affects the elderly satisfaction with psychological domain. The study recommended Promotion of health care provided to the elderly especially with regard to access to health care and the practice of physical activity. The Ministry of Labor and Social Affairs, in cooperation with the Ministry of Health can establish a special health center for the elderly to help them to get health care. As well as the establishment of a sports hall equipped to encourage the elderly to exercise of daily activities sports. Work of collective entertainment trips continuously to improve elderly mental and social state. Study should be conduct to assess satisfaction among the elderly residents in geriatric homes and others in their own about health care services (comparative study).

فاعليه تطبيق الممرضين للممارسات القياسية على العناية بالاطفال حديثي الولادة المصابين بمتلازمة كرب التنفس في وحده العناية بالاطفال حديثي الولادة في مستشفيات بغداد للاطفال التعليمية Effectiveness of Nurses Application of Standard Practices On Care of Newborn With Respiratory Distress Syndrome In Neonatal Care Unit At Children And Maternity Teaching Hospitals

اسم المؤلف: محمد عزيز حسن
اسم المشرف: اقبال غانم علي معلة
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: التمريض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت دراسة شبه تجريبية في مستشفيات بغداد للاطفال التعليمية للفترة من 28 من كانون الثاني الى الاول من ايلول لسنة 2014 لتقييم فاعلية تطبيق الممارسات القياسية للممرضين والذين يتعاملون مع الاطفال حديثي الولادة المصابين بمتلازمة كرب التنفستم تقييم الحاجة من | A quasi experimental(interventional) study was carried out at Baghdad pediatric teaching hospitals in Baghdad from the 28th of January to the 1st of September 2014 to assess the effectiveness of the application of standardized practices to nurses dealing with neonate with respiratory distress syndrome. Assessment of needs was done to evaluate the nurses practices towards neonates with respiratory distress syndrome in neonatal care unit(NCU) by using a special format which includes multiples questions related to nurses knowledge and practices for 10 nurses works at the private Nursing home.The result of the assessment revealed that the nurses have poor knowledge and practices towards neonates with RDS. A pretest test was performed before the application of the program for 35 nurses, three of them was out of the study sample.Observation their practices based on the standardized items which was prepared by the researcher and extensive review of the literature. A non - probability (purposive) sample of 32 nurses who work in the neonate intensive wards was chosen to be involved in study sample, and an educational program was designed based on the results and basic nursing guidelines related to care for neonate with respiratory distress syndrome, it consists of two parts : the first part consists of demographic data, and the second part consists of an observational checklist format related to the nurses practices in care of neonate with respiratory distress syndrome. The questionnaire was used to observe the nurses practices in the pre - test applied before the program started , post - test 1 immediately after the application of the program, post test 2 after 1 month from the post test 1,and a post 3 after 2 months from the second post test. The reliability of the questionnaire was conducted through a pilot study and validity through a panel of experts. The data were analyzed through the application of a descriptive statistics frequencies ,percentages ,and application of inferential statistical procedures which include Pearson correlation coefficient and paired test. The finding of the study revealed that the nurses practices towards neonates with respiratory distress syndrome were improved after the implementation of the program in most of the items of the post test 1 ,2,and 3 of the program, and there is a significant association between nurses' practices and their level of education in most of the program items, There was also a significant association between nurses practices and years of their experiences. The study recommended increasing knowledge and practices of the nurses working in the neonatal care unit through regular training courses, in addition to increasing the number of graduated nurses from the College of Nursing in this critical unit.

تاثير البرنامج التثقيفي التمريضي على معارف وممارسات الملاك التمريضي ازاء التاهيل القلبي لمرضى النوبة القلبية The Effectiveness of Nursing Educational Program On Nurses Knowledge And Practices Toward Cardiac Rehabilitation For Patients With Heart Attack

اسم المؤلف: احمد عجلان علاوي ناصر
اسم المشرف: حكيمة شاكر حسن
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: التمريض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت دراسة شبه تجريبية في مستشفى الشيخ زايد ومستشفى الصدر العام ومستشفى الكرخ العام ومستشفى الكرامة ومدينة الامامين الكاظمين الطبية ومستشفى بغداد التعليمي للفترة من الاول من ايلول2013 الى الاول من ايلول2014 تهدف الدراسة الى تقويم فاعلية البرنامج ال | A quasi - experimental design study was carried out at the AL - Sheikh Zayed hospital; AL - Sader; Al - Karkh ;AL - Karama ;Imameinkadhimein medical city and Baghdad Teaching hospital during the period from 1st September 2013 to 1st September 2014. The objective of the present study is to evaluate the effectiveness of nursing education program on the nurses knowledge and practice toward cardiac rehabilitation phase one for patients with heart attackThe program and instrument were constructed and developed by the researcher for the purpose of the study A random sample composed of (80) nurses was divided into two groups , the experimental group consisted of (40) nurses exposed to the nursing educational program and control group consisted of (40) nurses were not exposed to the program.Reliability of instrument was determined through the use of test and retest for the knowledge test and inter - observation for practice and the instrument validity was determined through a panel of experts. The instrument to measure the effectiveness of nursing educational program on nurses knowledge and practice included (30) items concerning the nurses knowledge and 58 items included nurses practice nurses practices toward cardiac rehabilitation phase one for patients with heart attack.The analysis of data was performed through the application of description statistic(Frequencies, Percents, and Cumulative Percentages , Mean of Score , Standard Deviation , Relative Sufficiency ) and Inferential statistical (Contingency Coefficients , Chi - Square test , Fisher to present the differences between the experimental and control groups. The results of the study shows that the nursing educational program regarding nurses knowledge toward cardiac rehabilitation program phase one is effective. They also shows that there is a good improvement with highly statistically significant differences between pre - post tests of experimental group in overall domains related to nurses practice in items of health assessment heart, assessment chest pain, cardiogenic shock, daily activity living and health education. The study concluded that the nurses working in coronary care unit have deficient knowledge and practices toward cardiac rehabilitation phase one for patient with heart attack. The study also confirms that the instruction oriented program on nurses in the experimental group is successful. The study recommended that the nurses staff can be encouraged and having motivation for being participated in the special training programs and conference to fulfill the nurses needs concerning defects and limitations ed in their practice toward cardiac rehabilitation phase one for patient with heart attack.

صعوبات تنفيذ مهارات الالوان لدى طلبة المرحلة الثانوية في انجاز اعمالهم الفنية وعلاقتها بالاضطرابات الادراكية البصرية

اسم المؤلف: الاء رضا عبد الحسين
اسم المشرف: ماجد نافع عبود الكناني
الموضوع العام: الفنون الجميلة
السنة: 2012
الموضوع الدقيق: التربية الفنية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد الادراك الحسي عملية تاويل الاحساسات تاويلا يزودنا بمعلومات عما في عالمنا الخارجي من اشياء, او هو العملية التي تتم بها معرفتنا لما حولنا من اشياء عن طريق الحواس, اذ يحتل موضوع الادراك اهمية كبرى لدى المختصين بالدراسات النفسية عموما والمهتمين بعلم النف

التشويه في جماليات تصاميم اثاث الشارع وسبل معالجتها Distortion In The Beauty Of The Design Of Street Furniture And The Methods To Remedy Them

اسم المؤلف: بان محمد شاكر الطائي
اسم المشرف: نوال محسن علي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: التصميم الصناعي
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تتناول الدراسة الحالية (التشويه في جماليات تصاميم اثاث الشارع وسبل معالجتها ) بصورة اكثر شمولية من مثيلاتها لما لها من اهمية في ذائقية المتلقي من خلال البعد الوظيفي والمظهري لاثاث الشارع مع الحفاظ على الهوية والخصوصية في التصاميم , وان مشكلة البحث تحددت | The Current Study deals with(distortion in the beauties of Street Furniture Designs and how to remedy them), in more comprehensive manner than other similar conditions, because of the importance to reflect in the taste of the recipient by the function and the visual aspect of street furniture with maintaining the identity and privacy in the designs , and the problem of the research was out lined in the following question : What are the aspects of distortion in the beauties of furniture used in local areas (street furniture) and methods of treatment ?As for the importance of the research , it was focused in raising the level of taste for beauty of the recipient by determining the distortion taking place in the beauties of the design with finding means to fix this distortion while the research aims to specify the concept and type of distortion taking place in the beauties of street furniture as for the research limits they were outlined by the subject of distortion in beauties of the design of street furniture units used in public areas and how to fix them , in Baghdad /(Alwaziriyah) for the duration(2009 - 2014).As for the procedures of the Research the descriptor method was employed with accrediting a questioner from, which specified the basic axis of analysis, and the researcher have found out the following important results : The distortion took place in the percentage and in some models with a distortion in the visual communication as a result of misuse as the metal ore was distorted because of the bad joining methods which was affected in the climate conditions in several models.The most important conclusions : When the designer ignores the design foundations and the relations the distortion appears unintentionally in the appearance of the industrial product and by choosing some types of ores and joining methods and failing to use good painting , this create is an obvious distortion in these units because they were exposed for different environmental and climate effects which have contributed in creating the distortion , and the research ended by offering a set of recommendations and proposals.

البعد الجمالي في التكوينات الابداعية للخط العربي Aesthetic Dimension In Linear Formations Creative Calligraphy

اسم المؤلف: كريم سالم الساعدي
اسم المشرف: جواد عبد الكاظم فرحان الزيدي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: الخط والزخرفة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعد مسالة الابداع والابتكار واحدة من اهم المسائل التي تشغل الانسانية في شتى مجالات المعارف والعلوم والفنون، فالابداع سمة العصر الحديث والطريق نحو النمو والرقي الحضاري، ولا يستثنى اي حقل معرفي او جمالي من الانشغال في قضية الابداع والابتكار فهما وسيلة التا | The issue of creativity and innovation is one of the most important issues of concern to humanity in various fields, Creativity characteristic of the times and the road towards progress and prosperity.To Aisttny any field of cognitive or aesthetic preoccupation with this issue, they are the means of meditation deep problems in order to develop solutions to the original and suitable.In Arabic calligraphy, which still indicates many of the questions and give a lot of fields of research is the study of dimensions of creativity is very important to deepen in the region to focus on the aesthetic and artistic objectives.When not studied this issue extensively, has directed the researcher to study through the four seasons, wondering in his first season for dimensional aesthetic that is available by formations linear, and attributes its transformation into a formality creative new leaves the context of traditional and maintains the rules discreet Arabic calligraphy.In the theoretical framework have addressed researcher in three sections to the concept of linear creative formations, as well as the characteristics of the formal and aesthetic and semantic configurable linear, and the effectiveness of Marja receive text language as a springboard aesthetic creative founder of the degree zero aesthetic in modern linear configuration.This included the third chapter action research to analyze the models selected sample and the approval of the orientations of the search, the according to a set of pillars appropriate to achieve the objectives of the research, and seal search a bid for the results showed the contribution of infrastructure design and processing of the contents of the text, and overlapping techniques races other technical in the incorporation of the dimensions of the aesthetic and creative in the configuration linear , as demonstrated by the conclusions of the possibility of achieving the objectives set for the search through the system prepared by the researcher and down to develop a set of recommendations and proposals.

تقويم تدريس الطلبة المطبقين في قسم التربية الفنية اكاديمية الفنون الجميلة جامعة بغداد Evai.Vation Teaching Practice Of Students In Art Education Depar'L'Ment Acadj!My Of Fine Arts Tjniymsity. Ot Baghd.Ad

اسم المؤلف: عبد المنعم خيري حسين
اسم المشرف: سعدي لفتة موسى
الموضوع العام: الفنون الجميلة
السنة: 1986
الموضوع الدقيق: التربية الفنية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Evaluation is considered as a vital factor in the·education.field.Preparation of the art teacher academically and the practical education, appliance in the high school is a funda11entai part in theoretical student preparation in four years duration.When any element of artistry educational occupational l does not exist, it may affect efficiency of (student/teache ) during practical application.us to study the practical aim~ The necessity requires from that they must exist in the behaviour of student/teacher during training perlod. The importance of research is to explode from the followingpoints : 1 I Academy of Fine Arts requires such&#39;study, this is ascientific study in the qualified field of art educa•tion.2 It shows the internal efficiency of Art Education Department in ~he Academy of Fine Arts··, Univer

البناء التشكيلي للرسوم الموجهة للاطفال The Formation Structure of The Painting For Children

اسم المؤلف: كاظم عبد الزهرة رسن
اسم المشرف: نجم عبد حيدر الجنديل
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: الرسم
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Developed countries got interested in comic stories after they touched the amount of their broad impact on the development of the child 's perceptions and influence on the growth of his character , and reached this limit interest forcing governments themselves to the legislation of laws that regulate and develop basic obligations and the rules of this art.It was a comic fount major supplying communities with knowledge and science and the publications that contain Comics spread widely distributed millions of copies and were conducted in the world hundreds of thousands of research studies and articles about this art , while the remaining Arab world and the Iraqi JH particular deals with this art has led to marginalize him , what was a major reason for the decline in the level of what is produced in this field of fees , in the Arab world is seen drawing for children as a limited role on decorating books and magazines is not seen critics and researchers to outcomes that will be drawing the bulk of them , but from the perspective of educational denial role art - making communities.From here came the attention of the researcher and its insistence on research in the history of this art and details and patterns , trends, and especially after it was discovered that the Arab Research and Studies in this regard are not available at all Never before an Arab researcher that the research in this field.Can be summed up what he had done researcher in this research are as follows : Researcher touched in the first quarter of the research to the research problem , and the spatial and temporal boundaries , and goals , and the definition of terms for a definition of the research linguistically ,in termly and procedurally.In the second chapter , which included theoretical framework , touched a researcher in the first section to the child's personality and how to grow , noting that growth determinants and reviewing the school when it came , in the fields of psychological and social science theories.While the second part consisted of comics history with a review of the most important patrons referring to the history of censorship art Alcolmx and laws enacted by governments because of the spread of this art is widely and people wanting it.In the third section reviewed the researcher styles and trends pursued by graphic artists are important in the world referring to a group of professional painters who left a clear imprint in the history of this art.While ensuring the fourth section a review of the history and active storyboards in Iraq , and was talking about the pioneers of this art in

المنظومة الضوئية وتغير المكان في العرض المسرحي العراقي Lighting System And The Change of Place In Iraqi Theatrical Show

اسم المؤلف: علي محمود محمد السوداني
اسم المشرف: قاسم مؤنس عزيز
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: الفنون المسرحية - تقنيات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

ما بعد الحداثة وانعكاساتها في سينوغرافيا العرض المسرحي العراقي Postmodernism And Anekasthe In Scenography Supply Iraqi The

اسم المؤلف: فيصل علي شرين الدوسكي
اسم المشرف: قاسم مؤنس عزيز
الموضوع العام: الفنون الجميلة
السنة: 2014
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد فن المسرح من الظواهر الحضارية الانسانية، اذ يوصف بالتطور على مستوى وجود الانسان بمستواة التاريخي، ويعد قديما على مستوى ظهور الحضارات الكبرى كما هو حال حضارة وادي الرافدين، ووادي النيل، والحضارة الاغريقية...الخ.ويمكن لقاريء تاريخ المسرح ان يلتمس ت | The art of the theater phenomena of human civilization , as described evolution at the level of the existence of the historical rights to its level , and is out of date at the level of the emergence of great civilizations , as is the case with the civilization of Mesopotamia and the Nile Valley and the Greek civilization... etc..A reader theater history to seek the development of the theater with Greek civilization and Romania to disclose material and references and institutions with beginnings and growth , which differed in which researchers , but what can we adopt in this thesis is what constitutes the structure PRESENTATION formal and dynamic game play as the development of methods Manifesting dazzling accompanied this growth of civilization , which can be described as the growth of tissue adopted all the knowledge available in the neighborhoods of the investment game play , including the science of optics and physical blocks and the lighting and colors and different... etc. Alaihamat of Science neighboring possible to utilize them in a product of game play.This as well as the evolution and transformation of aesthetically pleasing as a result of shifts in the structure of consciousness itself , and appointed to the nature of beauty and the beautiful , including changes to the historical level of movements and technical approaches , which as the variable information on the level of art history , or philosophy of art history , and is the idea of post - modernism , and their productions of the most important aspects of development and transformation in the taste of the Assembly, which swept the arts and literature , and all the creative structures of human civilization , including the art of theater that has been affected by and produced what we might call postmodern theater , which skips steps intellectually and technically.One manifestation of the modern theater transformation broad scenography theater , which was adopted approaches and trends of modernity , starting from Impressionist and passing Baltkaaabah and symbolic and Dadaism and the end Balsraalah , but that theater modernism influenced by the art of assembly and installation , which is one of the arts postmodern At the time of modernity itself and it is normal because the interval between modernity and Beyond separator default, can not be entirely watershed where things are a lot of overlap.The postmodern art , particularly theater , has achieved the outcomes of the task can be half to proceed with actors , which will reveal the institutions of post - modernism itself , and these outcomes with regard to the art of body art of the earth , and Video, and digital art , and these trends that form the structure of post - modernism , and found its place fertile in the art of theater in its system design.The Iraqi theater of theaters in bold and call variables which surprised theaters in the neighboring countries and the manifestations of this is what we find in the daring design creations , especially theater scenography of postmodernism.The researcher found that this trend did not take his scientific position and described dimension for analysis and exploration and drilling flour , despite the importance of the subject and seriousness because a shift in the overall environment for viewing theater , directed towards the form that builds scenography theater as an important center and active in PRESENTATION , which shows the importance of this research and the need for him as well as the goals and limitations cited by the researcher in the first chapter of this thesis and after the completion of the researcher to this chapter represented a systematic framework for the search , and then the definition of terms , the frame of mind of knowledge ( theory) is built on the following according to the detective.To enable the researcher of the mechanism of scientific research the exact terms founded and bases to reveal the secrets of this organization and to realize the verdicts Almazmrh and which was governed by a set of structures and cultural patterns , social, economic and even psychological linked Bmarjaaat doctrinal through First research of the second quarter , which was under the title ( the philosophy of modernity onwards ) of this section has been detected philosophy of modernity and their references and their origins and transformations to the postmodern.The second section devoted to ( artistic forms of art postmodern ) because the subject of modernity and beyond is the fabric represents all aspects of civilization kinds Mstdeih inclusiveness and interaction of all aspects of culture change that was philosophically and politically or scientifically or technically , and calls for change and openness to the new.The third section ( Alcinogravea and a mechanism to engage in postmodern theater ) reveals that the correlation between design and architectural Alcinogravea stage and the mechanism of performance and technology that have subscribed to a set of elements and mechanisms that shape the visual presentation of the playwright. Reading is the new scenography in postmodern theater allows for the recipient that explains the artwork in his own way , but re - formed , and according to his reading on what he sees and diversity of stylistic trends of the scenography depending on the variety of trends and styles in the era of post - modernism.After he finished his research researcher kits through detective theoretical , intellectual and technical moved to the third quarter , which represents action research as beginning with his community and his research samples and method , and the method of sampling Mentahja intentionality of the total samples of the title in question and the justification for the selection of samples.Finally, it was the fourth quarter , representing the results and conclusions reached by scientific research which has important consequences on the theoretical level analytical and exploratory and finally the level of future default.The researcher presented at the end of his research recommendations was for supplements that include interviews and research provided by the professors thought and literature, theater and the creators of the design at the College of Fine Arts Mtfdilan thankfully they are both professors Prof. Dr. Fadil (Sami Abdul - Hamid ) , Dr Fadil ( Fadel Khalil ) Dr Fadil ( Salah reeds ) , Dr Fadil ( Abbas Ali Jaafar ) Dr Fadil ( star Haider ) , Dr Fadil ( Hussein Altk ) , Dr Fadil ( Mohammed Abdel - Rahman al - Jubouri ) , Dr Fadil ( Aqeel Muslim).Finally, like the researcher a statement that art ( scenography ) postmodernism in the Iraqi theater is an art recently has been associated with the renaissance of the theater , after the arrival of creative artists from Europe , including the artist ( Kadhim Haydar ) and artist (Sami Abdel Hamid) and artist (Ibrahim Jalal ) and artist ( Fadel Khalil ) and artist ( Salah reeds ), which is an inexact art and composite difficult to interact with it and achieve results , making its employees do not complete the fingers stop per numbering they Mtfaalon in the level of their offspring quantitative and qualitative , and many of the constraints facing the working art Alcingravea , including constraints and objective thought , because theater postmodern disregards the form and terms of reference or foundations or private art techniques , each work has its style and its terms of reference and ideas that are different from the other work

معطيات المعرفة العلمية للمتحول التقني في المنهج الصناعي Personal Representative And Performance Between The Epic Theater And Gala Theater Offers In Iraq

اسم المؤلف: صلاح نوري محمود الجيلاوي
اسم المشرف: قدوري عراك صكر هدى محمود عمر
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: التصميم الصناعي
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The current study entitled (the given scientific epistemological knowledge for the technical transformation in industrial product), approached the subject of design in general, and modern industrial product design, in particular, which employed modern techniques and have new concepts and ideas, which was the main element in making significant changes in product appearances. the first chapter included the research problem which it concentrate about identifying the epistemological systems and structure and foundations, which allows us to understand the mechanism and relational components of industrial product , according to that knowledge that contribute to the activation of mental images and imaginative abilities of industrial designer, and reproduction of ideas that used in identifying the technical transformation for that product. According to that, the research problem has crystallized by these questions : 1. does the scientific knowledge has contributed in evolving the product design, and how techniques effected by the upcoming of scientific knowledge? 2. the extent to which the Awareness of industrial designer expertise cumulative knowledge gained through the stages of time?While the importance clarified through the data of scientific knowledge and the overlapping with art, since such interference can appears the values of artistic techniques of product design, so the detection of creative achievement system of scientific knowledge of pure mathematics, physics, chemistry, and technical aesthetic knowledge that appear in the design of industrial product which brings us to an account of the movement of pattern of the mental mechanism visualization and call for a stylistic vocabularyof industrial product to deal with, as it shows the followingimportant points : 1. The pattern movement of mental perception mechanism with which industrial designer generates from which new and innovative ideas based on the accumulation of knowledge, which stores them in memory and call the vocabulary of industrial product and stylistic interaction. 2. The scientific construction mechanism of knowledge and expertise to the industrial designer can be the organization that operates the juxtaposition actor and moving in the structure of the fabric with Other cognitive knowledge. The study aimed to detection and identification knowledge for scientific knowledge and its relationship to technical transformation and their implications in the design of industrial products.And the study formed of four chapters, the first chapter is the research problem and its importance and its objectives and its borders and determine the terms. the second chapter contains on previous studies and theoretical framework , which included four sections. First section dealt with the aspects of philosophical scientific knowledge , and ensure the second section shifts the intellectual , philosophical and tracks applied , and the third section , entitled the concept of technical and its applications in the design , and the fourth section has dealt with the concept and awareness of modern aesthetic and the crux of this chapter, a set of indicators has dealt with Chapter III action research and methodology, as the researcher adopted a descriptive approach in the processof describing and analyzing models of the sample , while the Chapter IV describes the models and analyzed by form axes of analysis developed by the researcher , and the results and conclusions.Chapter four includes models described and analyzed by analysis axes form developed by the researcher, and the results and conclusions. the most important results that emerged from the study are as follows : 1. Diversity in the techniques of high - quality has contrebute in the devices used to distinguish according to specialties.2. the use of new materials in not used of industrial products with a new characteristics, helped achieve the concepts of reductionism and simplicity, durability and ease of use and the possibility of high composition.3. The presence of correlation between technique based on scientific knowledge and applications that have emerged in the experimental applications of industrial product which suited with the mechanics of the user, allowing him to a great deal of activities embodied in scientific knowledge and scientific applications.4. the Change in human behavior and activity through the interaction of functionality, which has contributed to the aesthetics and functionality of the product as a result of the employment of scientific knowledge and its implications, which appeared during the experimental applications. The most important conclusions that emerged from thestudy were : 1. The technologies evolve continuously invested scientific discoveries, and there is no doubt it is one of the effective means to overcome the problems facing the process of development in the industrial products , and this continuity in progress constitutes an important factor in the accumulation of knowledge, which in turn lead to progress and impressive innovation overall of human activities in those products. The technical evolution is an interconnected and connected to start one of them up where the other and in spite of the diversity of techniques between the equipment and the materials and information , but it all involved in creating the products of design will transfer industrial products from traditional character to the modern technical nature, which it need to integrate them , to systems more sophisticated , namely, ( Smart systems )2. The adoption of advanced scientific knowledge has been led to intellectual shifts in the designs of industrial products in terms of function and aesthetics, which gave the impression that intellectually to the role of knowledge in the development of the social life of the user.3. the scientific knowledge Contributed to improved in making changes to the concepts held by the industrial designer See Through accreditation and keep up with new technologies, and this will be achieved throgh the product which it has cognition communication between that and the user, and this is reflected in the designs of industrial product to suit the needs and functional requirements and aesthetic that fit with the taste of the user.4. That most of the techniques employed in contemporary designs of industrial products and its evolution, which is characterized by a technical Nanotechnology and technical tactile and digital technology and radiation, artificial intelligence and pooling of modern materials, which contributed to a change in systems of formal and functional designs in industrial products

جماليات توظيف برامجيات الحاسوب في سينوغرافيا العرض المسرحي The Aesthetics of Employing Computer Softwares In The Scenography of Theatrical Show

اسم المؤلف: راوية جبار حسن
اسم المشرف: محمد عبد الرحمن الجبوري
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: الفنون المسرحية - تقنيات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان المسرح يعد حصيلة لتكامل النص مع الاداء الصوتي والحركي للممثلين ومدى انصهارهم مع المنظر والاضاءة والازياء، وهنا تظهر امكانية استخدام برامج الحاسوب في جزء من المنظر والمؤثرات الصوتية والضوئية وباستخدام تكنولوجيا العرض هذه مع بعض برامج الحاسوب مثل ( 3D G | The theater is considered as a product of text integration with voice and kinetic expression of the actors and their interaction with the scenery, lighting and costumes. Hence, we can find a possibility of using a computer in a part of the scene, sound and lightening effects. Using display technology by some computer programs such as (3D Graphics) and (Virtual Reality) can configure the sense of depth and different views that commensurate with the desired show. It is also characterized by dynamic capabilities which are not available in the traditional decor based methods. For example, dynamics allow making a change on the display elements with taking advantage of other video presentations to be an integral part of the background. The actual or real effects are to invest the non - final maximum energies for the PC in the creation of artwork tools and symbols, reproducing voice and image so that it its impact on viewers (sometimes) would exceeds the reality and beyond sometimes. The actual effectsoffer its tools to the computer user; would make it dive into an experience that is so close to reality. So the researcher has divided her research into four chapters : Chapter I - Research methodological framework : it includes the research problem, its importance, the need to it, its goals and limits. It ends with defining the following terms : (beauty, employment, software, computer, theatricalscene) Chapter II - it contains a theoretical framework for the research and previous studies, including four sections : First : the aesthetics of modern experiments in the formation of the scene. Second : technology and experimental trends.Third : the aesthetics of design by the computer (Digital Arts). Fourth : lighting devices used in modern theater. Finally, this chapter shows the indications that the theoretical framework ended up with.Chapter III : it includes the research procedures which the whole research was consisted of. It is limited to the Iraqi and Arabic theatrical performances. The samples of the research on the other hand were deliberately chosen by the researcher and for the following rationales : 1. The samples represent the research problem, its importance and its objectives. 2. The extent of disparity between the theatrical directors which the researcher has highlighted their scenes on a play that was embodied by their theatrical shows. 3. Archival sources of theater shows provide the sample of the research. Regarding the research methodology, the researcher has adopted the analytical approach in the presentation of theoretical framework, also adopted the descriptive approach in the presentation of samples brought by the whole research.As for the research tool, the researcher relied on the indicators that resulted from the theoretical framework.The research samples are : 1. Adding Machine directed by (Ronald Wells)2. theatrical play (Play) directed by (Lance Jarafa)3. The use of technology in the play of (Othello) directed by (Salah Qasab).Chapter IV has included research results and conclusions, then the list of resources and supplements. Finally, it ends with an English abstract.

نظريات الاتصال الحديثة واسقاطاتها الفكرية على المنتج الصناعي The Modern Communication Heories And Their Intellectual Impact On The Industrial Product

اسم المؤلف: جاسم احمد زيدان
اسم المشرف: شيماء عبد الجبار حميد العبيدي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: التصميم الصناعي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Industrial design is regarded as an intellectual and cognitive product that is developed with accordance to the analytical awareness of man interpreting accordingly the external existence with the means of senses which human and other creature have. The senses are the window overlooking the external world and the environment. The form the beginning of mediation and observance and concentration and the formation of depictions which work according to the innovative imagining.Therefore the industrial design is regarded as one of the important pivots of the fine arts. These pivots revolve around the bases of how to convey meaning or the design of the idea to the users or the receivers in the clarity with hindrance to the understanding.The language of visual communication which scientific ad practical bases are formed according to the modern theories of communication. It is the language of modern communication of signs and relations are connected together. The modern theories of communication have offered developed system of communication to the user. But these face the problem of awareness and culture in the confrontation of the developed industrial products (multi - purpose digital devices) as means of communication in finding utilities.Upon the above, the researcher preferred to found the problem of his study through revealing the reflections which are formedby the modern theories of communication on the industrial product of the electronic device (Smart TV, Smart Camera, interactive board, Tablet PC, Smart phone).The study aims at the following : 1 - Investigating the modern theories of communication ad their intellectual impacts on the industrial product.2 - Analysis of sample of modern industrial products to know the effect the modern theories of communication in the development of product.In order to verify these objectives, the researcher founded a theoretical framework that tackles the philosophical backdrops to knowledge and communication and put the following (3) inquiries : First inquiry : The relationship between sensual visual realization and communication.Second inquiry : the modern theories of communication and their application fields.Third inquiry : the aesthetic thinking and its relations to the theories of communication and their impact on the industrial product.The researcher comes up with a number of indications which resulted from the theatrical framework depending I the design of the instrument which is the content analysis form of the industrial product specified in the sample.In order to determine the modern theories of communication that could be concord with the samples of the study, the researcher made a survey of the opinions of the experts in these theories. They referred to him to the theories of (McLuhan - Diffusion and innovation - selective effect - developmental technology).The research community was confined in the products of Samsung ad Sony institutions. The researcher chose a sample of (5) products after consulting the experts in the field of industrial design. The, these samples were analyzed according to the analysis form prepared for that purpose, which was subjected to the validity coefficient test by exposing them to the group of experts.Based on the analysis conducted for the sample, the researcher came up with a number of findings : 1) The structure of the sample was characterized by an apparent functional value and utilization which the user can touch and enjoy in their aesthetic function.2) The sample was characterized by digital products with aesthetic value especially in relation to the outside form of these products which contain attractive points emerging from the utilizing features for every type. This created a form of power in the taste.3) There is a clear reflection of the modern theories of communication on the functions of the industrial productsof sample in the functional and utilizing ad aesthetic aspects.The most important findings are the following : Upon these conclusions the researcher made a number of recommendations which are :

البنية التشكيلية لشكل الثور في النحت العراقي المعاصر Plastic Structure of Ox Shape At The Contemporary Iraqi Sculpture

اسم المؤلف: منى يونس عبد الجبار يونس عبد الجبار الجنابي
اسم المشرف: محمود عجمي جاسم الكلابي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: النحت
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study deals with study of ( Plastic structure of OX shape at the Contemporary Iraqi scdlpture ) , it comprises Four Chapters , First Chapter to demonstrate the Study Problem , its significant , its necessary , its target , and determine most important idiomsmentioned at the study.the study Problem inciudes raise limited questions about these study , and answering to that questions through debate main subject that connect to effect of human being at ancient period which is appearin the modern Art action as atopic subject submitted ti study through embodiment in modern Iraqi sculpture actions , and it should be adopted for intellectual and artistic composition to identify its essential.second chapter ( theoretical framework )comprises three topics , first topic deals with the concept of structure , second chapter concern with Iraqi environment effect on composition of OX s shape by course of time on distant ages , third chapter concernsstructure of OXs shape at modern art. chapter three includes study approach , in which researcher review Research population and its sample selection in addition to research tool and its approach. chapter four Ends with research results , conclusions , recommendations , andsuggestions , some of the results that study reach are : - 1 - Add aesthetic value for art action that embodied of OXs shape which has the content that interject subjective and objective of expression through forming of shape till it reach to a limited manner which hold that value , in addition to the intellectual value.2 - It is possible to set a value evaluation that on the vision simples of the OXs shape that has a determinants that embody by external form which determine the simple value that the action refer to it in this manner or embodiment.3 - the vision formulation of OXs shape in some samples has a decorative composition dominated by the nature of old Iraqi traditions that has its own reflect influence on such a simple of contemporary Iraqi sculpture.4 - Some of the OXs shape has a subjectivity plastic composition that alters the shape construction an aesthetic value more than objective one.

الصورة الطقسية في المسرح العراقي المعاصر : عادل كريم انموذجا Ritualistic Image In The Contemporary Iraqi Theater. (Adel Kareem As A Sample

اسم المؤلف: سعدية نوري محمد
اسم المشرف: سعد عبد الكريم خيون
الموضوع العام: الفنون الجميلة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

الزخارف الاجرية في القصر العباسي وامكانية توظيفها في اعمال الطلبة : النحت على الحجر انموذجا The Stony Ornaments In Al - Abbasi Mansion And The Way of Using It In Student'S Workers Sample : Sculpture On Stone

اسم المؤلف: منذر عبد المنعم محمد الطائي
اسم المشرف: هاني محي الدين محمد النعيمي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: التربية الفنية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The first chapter deals with studying the problem, the aims, the search limits and idioms and the production of the problem as follows : In general, the Islamic Art is special in its ornamental aspect. In this way the ornaments in all its kinds (Engendering, Vegetarian, Script and animal) becomes main part used a lot in most Artistic products in Moslems especially when in began to appear in Islamic building such as mansions, mosques, schools, all worship houses and holy places during the life of the Islamic city.The ornament with all its forms and shapes is one of the human aspects in Islamic Arab Art. Whether, it is important formed in ornamental lines or various ornamental shapes.One of the historical building that has still been in the city of Baghdad is "Al - Abbasi Mansion on the bank of Tigris which expresses the fabulous building in achievements and the high use of the stone ornament that decorated the walls from the outside and inside.To keep attractive value and the whole heritage of this mansion which expresses that technique and the way of pressing on stone by the ancient Iraqi sculpture all over the famous civilization of pesopetiemia that is used nowadays as a basic way of building the new ornament.And through the researcher's study in Sculpture's hall in the department and meeting a number of students and noticing their works, he finds that the students have no intellectual and practical skills about sculpture on stones because they don’t have enough knowledge about the way of direct achievement, they don’t know how to practice this kind of technique before.So the researcher wants to use the ornamental units in the students products in Collage of Art / Sculpture department in order to investigate about the answer of the following question of this study which is : Is it possible to use the ornaments from AL - Abbasi Mansion in sculpture works for Fine Art students / Plastic department/ sculpture part.The importance of answering the question in brief is that : - 1 - It is useful to the specialist sculpture in designing field , Arabic script , ornamental.2 - Supporting the calculums about designing the ornamental forms and the way of achieving them by using hellan stone or, gypsum marble in decorating the walls of buildings.The aim of this research is to be able to know the ornamental units of A L - Abbasi mansion and the ability to use it in the products of students in fine art college / plastic Department / sculpture part through : A - Making a training program to make students of Fine Art / sculpture part to know designing skills and achieving the ornamental units on stones.The researcher puts two supposals as follows : - 1 - There is no difference that has mathematical way on 0.05 between the students medium marks ( experimental group) who have been trained on sculpture on stone and practicing it through the works of third year students sculpture part according to program about their answers for items of intellectual total test before and after.2 - There is no difference that has mathematical way on the level of 0.05 between the students medium marks (experimental group) who have been trained on sculpture on Ahillan stone and practicing it through the work of third year students / Sculpture part according to the program about their answers for the items of practical total before and after and limits the research in : - The ornamental units that has been in AL - Abbasi mansion in Baghdad, the students of third year plastic sculpture part / Fine Art College / University of Mosul 2013/2014.The second chapter includes the visual aspects and the previous studies and the sources that are put in writing to get benefit from and the study of the researcher.To some points to make use of whenever he needs that through his paper some parts are united to be easy to study such as : a brief look on the history of AL - Abbasi mansion ornament , stony ornaments in AL - Abbasi : mansion.A historical summary about the appearance of Islamic Arab ornaments , the philosophy building the ornamental design. In structional design. The third chapter involves the research equivalents and the experimental.It limits the study experimentally and making a training program according to the scientific calculums for the scientific research. In this way the limits the community of the research and the experiment. It deals with community which the experimental about ten(girls and boys students) in the third year /sculpture / department / University of Mosul.The tools of the research include two tests the intellectual and the skillful before and after. The researcher used the scientific steps in finding the honest use of tools.Farther more , the research designs a form to evaluate the skillful performance , lastly , the researcher puts keys for answering , then he gets a help from two scoring who are specialist in the field of sculpture.The fourth chapter includes the most important results , it is obvious that there is a good achievement in the intellectual and skillful comprehension through the success in the studentsknowledgeable and skillful performance after training the students to practice sculpture on stone , they do very well. This shows the activate way in using this suggested program for this study.The researcher concludes that there is weakness in the knowledgeable part of the level of the 3rd year students/Fine Arts - College - University of Mosel.Probably the reason is that the academic calculums lack the students specialty because their training on clay materials only makes them lose the achieving skill of sculpture on stone in all its different kinds such as : stone marble and etc..1 - the lack of using work techniques (Sculpture tools) because of the lack getting the exact skill to Plastic Art students that leads to weakness in the students learning.2 - The lack of training students on sculpture to use different thing such as : stone, gypsum, marble and etc. makes them lose their skillful experiences.3 - Most of the teachers are old artists lack the practical experience for the new methods of teaching and technique of using the machines and the lack of using visual aids in front of the students.The researcher advised through his conclusions the followings : - 1 - Increasing the practical and the special part of both teacher and the students , through the continuous evaluation of the calculums and modernize them continuously.2 - Using this programed in this study to teach sculpture on stone for sculpture part and all other departments.3 - Opening complete modal workshop containing different materials and the useful tools in sculpture on stone, marble gypsum , wood ,etc……The research ends with some suggestions , then the scientific resources that can be used by the researcher

نظرية الحل الابتكاري TRIZ وانعكاساتها في التصميم الصناعي Theory of Inventive Solution (TRIZ) And Their Repercussions In Industrial Design

اسم المؤلف: محمد علي حسين القيسي
اسم المشرف: قدوري عراك صكر
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: التصميم الصناعي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The importance of the research is the importance of the theory of (TRIZ), which are based on the scientific method resulting from the analysis of the content of more than two million patents, founder of (TRIZ) was Genrich Altshuller, it assumed that the principles of general creative form the basis of the output of creative and that these principles can be identified and transferred to others to make the innovation process more amenable to learn. It recognize as the most important theory of the task, as adopted in more than (50) major international companies, including Company (Boeing for the aircraft industry and Toyota Motor).To summarize the research's problem, there is no publication of TRIZ theory concerning industrial design, the goal of the research is to characterize and employ a matrix of contradictions for the purpose of contradictions matrix Created especially to industrial design. The search Limit to traditional TRIZ theory (1973), which includes a matrix of contradictions, which contain in its composition, the forty principles and the 39 engineering standards as objectives limits, the research contain its theoretical framework of four sections, the first section dealt with the creativity and its theatrical concepts. This has been confirmed that the creativity and innovation cannot be taught or trained or acquisition, as well as the concept of creativity after the first quarter of the last century which showed that creativity could be educated and transformed.The second part of the research explain TRIZ theory and nine of their instruments, The tool matrix contradictions have been allotted for the Study of particular axis as the basis for the study.The third section has dealt with the contradictions and i matrix Included on the study of the concept of contradictions besides reviewed the (39) engineering standards and forty principles, the fourth section studied eight previous TRIZ's studies.The most important findings of the study was achieved the contradictions matrix model of industrial design, which Includes on (20) engineering standards and (40) principle. These principles characterized of the examples used to explain them was new which is taken from the field of industrial design and pro
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