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تاثير الاصابة بداء المقوسات الكوندية على المستويات الهورمونية والمدورات الخلوية خلال فترة بلوغ الانسان في محافظة بغداد == The Effect of Toxoplasmosis On Hormonal And Cytokines Levels During Human Maturity In Baghdad Province
Author name:
ياسمين رياض عبد الكريم الخناق
Supervisor name:
صباح ناصر العلوجي | خولة حوري زغير
General topic:
Biology
Specific topic:
Microbiology - Parasites
Degree:
Master
University:
University of Baghdad
Language:
English
University location:
Baghdad
First pages:
24T2668 - p.pdf
Abstract:
داء المقوسات الكونديه مرض عالمي الانتشار حيث يصاب معظم الاشخاص ذوي المناعة الجيدة بطفيلي المقوسات الكونديه وغالبا دون ظهور اعراض. ان الهدف الرئيسي لهذه الدراسه هو فهم الفروقات الجنسيه, الهرمونيه والمناعيه في سن البلوغ في الاشخاص الذين لديهم اجسام مضاده نو | Toxoplasmosis is a worldwide disease where most healthy, immunocompetent individuals infected by Toxoplasma are almost asymptomatic.The primary goal of this study is to perceive the hormonal and immunological sex - differences in puberty age who have positive anti - Toxoplasma IgG specific antibodies. The secondary goal is to inspect the endocrine - immune interaction in these persons by detecting the effect of testosterone and oestradiol hormones level on cellular immune response namely, IL - 4, IL - 12. From the first of November 2012 till the end of April 2013, 303 blood samples were collected from apparently healthy male and female students of Al - Erfan, Ignadeen and Algawahery schools and Baghdad University, Both sexes where divided into two age groups : group (A) which included subjects with age range (12 - 15) years old and group (B) which included subjects with age range (16 - 19) years old. All serum samples were tested for toxoplasmosis by using Latex agglutination test and ELISA anti - Toxoplasma IgG antibodies test. As well as, all serum samples were tested by using ELISA technique for detection of serum mean concentration of testosterone, oestradiol hormones, IL - 12 and IL - 4. The results revealed that 107/ 303 (35.31%) of the studied subjects showed seropositive toxoplasmosis, 60 males and 47 females of 107 positive samples showed high significant (p<0.01) differences in comparison to uninfected subjects. Males group B have recorded the highest percentage 34(41.46%) of the infection. Positive association was found between toxoplasmosis and testosterone level in asymptomatic toxoplasmosis cases compared to uninfected group. high mean concentration of testosterone in toxoplasmosis infected males recorded (15.03± 1.04 ng/ml) and (12.4± 0.91 ng/ml) in groups A and B respectively, in comparison to control group which recorded (8.03± 0.78) and (9.86± 0.83) in groups A and B respectively. Also toxoplasmosis infected females revealed high levels of testosterone hormone which represented (4.83± 0.06 ng/ml) and (2.55± 0.03 ng/ml) in groups A and B respectively, with a significant (p?0.05) differences between them, while the control group recorded (0.10± 0.02 ng/ml) and (0.90±0.03 ng/ml) in group A and B respectively.The present study showed a significant (P?0.05) decrease in the mean concentration of oestradiol E2 hormone in toxoplasmosis infected males and females in comparison with uninfected ones. E2 mean concentration was (41± 2.48 ng/ml) and (56± 2.91 ng/ml) for male groups A and B, respectively, in comparison to control group which recorded (67± 2.08 ng/ml) and (74± 2.42 ng/ml) in group A and B respectively, while it was (188 ± 12.48 ng/ml) and (196 ± 16.52 ng/ml) for female group A and B respectively, in comparison to control group which recorded (221±12.09 ng/ml) and (233± 15.63 ng/ml) for group A and B, respectively.The mean concentration of E2 hormone in toxoplasmosis infected females according to their menstrual cycle showed low levels in ovulation, late follicular and luteal phases, which represent (37.5 ± 2.59 ng/ml), (131 ± 16.7 ng/ml) and (76± 3.92 ng/ml) respectively, while the mean concentration of this hormone in uninfected females was (52.4± 2.88 ng/ml), (271.6± 21.04 ng/ml) and (196.2± 12.37 ng/ml) in the three phases of menstrual cycle. This study showed high significant (p?0.05) level of IL - 12 in both males and females with latent toxoplasmosis in comparison with free - toxoplasmosis groups. The mean concentration of IL - 12 in infected males was (4.75 ± 0.88 pg/ml) and (4.12 ± 0.69 pg/ml) in male groups A and B respectively, in comparison to control group which was (2.86± 0.53 pg/ml) and (2.46± 0.62 pg/ml) in groups A and B respectively, while it was (5.60 ± 0.12 pg/ml) and (6.04 ± 0.26 pg/ml) in infected female groups A and B respectively, in comparison to control group which recorded (3.32± 0.89 pg/ml) and (4.27± 0.15 pg/ml) in group A and B respectively. IL - 4 recorded quite elevated level in toxoplasmosis infected males (groups A and B) which was (15.09 ± 0.92 pg/ml) and (17.67 ± 0.78 pg/ml) respectively, in comparison to control group which recorded (13.89± 0.84 pg/ml) and (14.92± 0.69 pg/ml) in groups A and B respectively, Meanwhile the mean concentration of IL - 4 in toxoplasmosis infected females showed mild elevation in both groups A and B which was (15.14 ± 0.84 pg/ml) and (16.06 ± 1.13 pg/ml) respectively, in comparison with toxoplasmosis free subjects which recorded (16.53± 1.22 pg/ml) and (15.15 ± 0.97 pg/ml) with no significant differences between them. Interactions between the endocrine and immune systems may mediate sex differences in response to toxoplasmosis infection.
