التاثيرات السمية لجسيمات الفضة النانوية على بعض الاعضاء لاناث طائر السمان Coturnix coturnix : دراسة نسجية وكيموحيوية == Toxic Effects of Nanosilver Particles On Some Organs of Female Quail (Coturnix Coturnix); Histological And Biochemical Study
Author name:
عبير ساجد عبد علي
Supervisor name:
غزوة درويش النقيب
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
Arabic
University location:
Baghdad
First pages:
24T2756 - p.pdf
Abstract:
تضمنت هذه الدراسة التعرف على تاثير جسيمات الفضة النانوية في نسجية كل من الكبد والطحال والكلى فضلا عن تاثيرها في وظائف الكبد والكلى من خلال قياس بعض انزيمات الكبد المتضمنة (AST & ALT) وقياس مستوى اليوريا والكرياتنين (Urea & (Creatinine, فضلا عن تاثيرها في | This study was included to identify the effect of silver nanoparticles on histology of the liver, spleen and kidney, as well as it's effect on functions of the liver and kidneys through the measurementof some liver enzymes included (AST and ALT) and measurement of urea and creatinine levels, as well as the effect of silver nanoparticles in the weight of studied samples, numbers and sizes of the eggs were produced through the exposure periods.(45) quail (females) were collected from agricultured research center in Abu - Ghraib, divided into (4) groups including : T1 (12 quails were exposed to 4ppm), T2 (12 quails were exposed to 8ppm) and T3 (12 quails were exposed to 12 ppm) of nanosilver particles solution for (60) days.As well as control group T4(9 birds were exposed to distilled water).After the end of the exposure period samples were sacrificed by discapitation of the neck, and blood was collected for the purpose of serum separation which required for biochemical study, samples were dissected and fixated with Bouin's fluid, histological slides were prepared and stained with Eosin - Hematoxylin.Biochemical study were showed significant decrease in the level of liver's enzymes (AST & ALT), urea and creatinine. This decrease was elevated when the concentration was increased, as well as significant decrease in each of sizes and numbers of the eggs were produced through exposure periods and non - significant decrease in the body weight.Exposed groups with silver nanoparticles showed histopathological alterations for each of liver, spleen and kidney, in liver included congestion of blood vessels, infiltration of inflammatory cells, exudate, appearing of concilman bodies, fatty degeneration in most of hepatocytes which progressed to fatty changes in most of samples were exposed with 12ppm, Hyperplasia for each of blood vessel walls and capsule, atrophy of hepatocytes, and appearing of giant cells, while kidney sufferd from hydropic degeneration in most of renal tubules hemorrhage for each of cortex, medulla and glomerulus, hyaline degeneration of tubules, amyloid precipitation for each of glomerulus and blood vessels, sloughing of epithelial cells of renal tubules from basement membrane, hypertrophy of renal tubules with hyperplasia for cells of tubules and atrophy of renal tubules.Spleen tissue showed decreased in sheets of white pulp and lymphocyte numbers red pulp cells. Severity of these effects varied depending on individual differences between samples and concentration of silver nanoparticles, which increase in samples were exposed with highest concentration.
