الظهور الكيميائي النسيجي المناعي للاوستيوكالسين، OC عاملا لنمو المحول بيتا واحد TGF - B1 وبروتين الشكل العظمي سبعة BMP - 7 في خلل التنسج الليفي والورم الليفي المعظم في عظام الفك : دراسة مقارنة == Immunohistochemicalexpression of Osteocalcin, Transforming Growth Factor Beta - 1And Bone Morphogenetic Protein - 7 In Fibrous Dysplasia And Ossifying Fibroma of The Jaw Bones Acomparative Study
Author name:
فرح غسان ابراهیم
General topic:
Dentistry
Specific topic:
Oral and Maxillofacial Surgery
Degree:
Master
University:
University of Baghdad
Language:
English
University location:
Baghdad
First pages:
20T383 - p.pdf
Abstract:
Fibrous Dysplasia & Ossifying Fibroma of the jawsare maxillofacial fibroosseous lesions sharing anoverlapping clinicopathological characteristics. This can be diagnostically challenging for pathologists& surgeons. It is important to make the distinction because of differences in their clinical behavior, modes of treatment and prognosis.Osteocalcin is an immunohistochemicalmarker; secreted by osteoblasts and its high serum levelsare correlated with increased bone mineraldensity. It is therefore, used as biomarker for boneformation process and also has a role in regulationof osteoblast function.Transforming growth factor beta - 1 is a multifunctional regulator of cell growth which will either stimulate or inhibit proliferation of mesenchymal cells depending on the presence of other growth factors. It is secreted by osteoblasts and is very abundant in bone matrix. Bone morphogenetic protein - 7is a member of transforming growth factor - b superfamily; it is widely expressed during embryonic growth, and is an essential morphogen in renal, skeletal, and eye development.Aims of the study : This study aimedto evaluate and compare the Immunohistochemical expression ofosteocalcin, transforming growth factor beta - 1 andbone morphogenetic protein - 7in the pathogenesis of Fibrous dysplasia and Ossifying fibroma of the jaw bones,correlate the expression of the aforementioned markers in these lesions.Analyze theimmunohistochemical expression ofosteocalcin, Transforming growth factor beta - 1 & bone morphogenetic protein - 7 inFibrous dysplasia & Ossifying fibroma, in order to assess its potential role in differentiation between these two disease entities.Materials&Methods : A total of 30 retrospective formalin - fixed, paraffin - embedded tissue blocks were included in this study, 15 were diagnosed asFibrous dysplasia of the jaws and 15 were ofOssifying fibroma of the jaws. Animmunohistochemicalstaining method using ofosteocalcin,transforming growthfactor beta - 1 andbone morphogenetic protein - 7 monoclonal antibodies were performed.Results : The results revealed that the majority of cases (73.33%) were females for each Fibrous dysplasia &Ossifying fibroma. the age range was (8 - 35)years for Fibrous dysplasia and (7 - 50)years forOssifying fibroma. For Fibrous dysplasia most cases presented in maxilla (66.76%) while for Ossifying fibroma most of the cases presented in mandible (73.33), with more predominant Fibrous dysplasia cases in molar area (60%) and more presented Ossifying fibroma cases in premolar & molar area (33.33%). Statistically significant difference was found between Fibrous dysplasia&Ossifying fibroma cases regarding jaws & site distribution (P=0.02&0.04) respectively.Osteocalcin positive immunohistochemicalexpression was found in fibroblast - like cells in 4 cases(26.66%) of Fibrous dysplasia and in 7 cases (46.67%) of Ossifying fibroma, according to Chi - square test the result showed statistically significant difference regarding osteocalcin expression in Fibrous dysplasia &Ossifying fibroma (P=0.04). Transforming growth factor beta - 1 was positivelyexpressed in 8cases (53.3%) of Fibrous dysplasiaand 10 cases (66.67%) of Ossifying fibroma. Bone morphogenetic protein - 7showed positive expression in 2 cases (13.3%) of Fibrous dysplasia and 4 cases (26.7%) ofossifying fibroma.Statistically non - significant difference regarding the immunohistochemicalexpression of transforming growth factor beta - 1 and bone morphogenetic protein - 7in Fibrous dysplasia &Ossifying fibroma. Moreover significant correlation was found regardingtransforming growth factor beta - 1 expression in response to bone morphogenetic protein - 7in Fibrous dysplasia (P=0.03)and osteocalcin expression in response totransforming growth factor beta - 1 in Ossifying fibroma (P=0.01).Conclusions : Transforming growth factor beta - 1 expressin the majority of Fibrous dysplasia &Ossifying fibroma cases indicate its important role in the process of osteogenesis. The significant correlation seen regarding the expression of some of the studied markers with each other suggest their cooperative role in the pathogenesis of Fibrous dysplasia &Ossifying fibroma
