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تاثير مستخلصات الشاي الاخضر على مسببات امراض انسجة الاسنان الرابطة (بكتريا البورفايروموناس اللثوية وبكتريا اجريجاتيباكتر اكتينومايستيمكومتانس) : دراسة مختبرية == Antibacterial Effects of Green Tea Extracts On Periodontal Pathogens (Aggregatibacter Ctinomycetemcomitans And Porphyromonas Gingivalis) (In Vitro Study)
Author name:
ميس جمال مجيد
Supervisor name:
سيف سهام سليم
General topic:
Dentistry
Specific topic:
Around the Teeth Diseases
Degree:
Master
University:
University of Baghdad - Faculty Of Dentistry - Department Of Periodontics And Peridontics
Language:
English
University location:
Baghdad
First pages:
20T386 - p.pdf
Abstract:
المقدمة : الشاي الاخضرهو واحد من المشروبات العلاجية القديمة والشعبية المستهلكة في جميع انحاء العالم، وهو مشتق من ورقة للنبات "كاميليا سينينسيس", يمكن ان يحضر كمشروب، والذي يمكن ان يكون له العديد من الاثار الصحية الشاملة او "مستخلص" يمكن الحصول عليه من الاو | Background : Green Tea is one of the most ancient and popular therapeutic beverages consumed around the world, it is made from the leaf of the plant “Camellia sinensis”. It can be prepared as a drink, which can have many systemic health effects or an “extract” which can be made from the leaves to be used as medicine. Green tea is reported to contain thousands of bioactive ingredients including catechins, catechins found in the tea have shown promise for having antimicrobial effects.Aim of study : to test the effect of green tea extracts on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in comparison to 0.2% chlorohexidine gluconate and distilled water in vitro.Materials and Methods : plaque samples were collected from 20 patients with no signs of any systemic disease suffering from chronic periodontitis with probing pocket depth of at least 6 mm, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were isolated and diagnosed according to morphological characteristic and biochemical test. Green tea leaves were extracted by using water and alcohol. This study involved two experiments in vitro concerning the effects of green tea extracts on these bacteria in addition to analysis of the extracts to determine the concentrations of the catechins in each extract. The first experiments involved testing the sensitivity of A.a and P.g to different concentrations of the extracts in addition to chlorhexidine gluconate 0.2% and distilled water using agar well diffusion method, the second experiment involved determination of the minimum inhibitory concentration of the extracts that inhibits the bacterial growth and then determination of the minimum bactericidal concentration of the extract that was requires to kill the bacteria. The present study also involved laboratory analysis of green tea extracts using high performance liquid chromatography (HPLC).Results : Both green tea extracts were effective in inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans using agar well diffusion method, 90% and 100% concentrations of alcoholic extract showed larger inhibition zones than chlorhexidine gluconate 0.2% with statistically significant difference, CHX showed higher inhibition zones than all aqueous extract concentrations.The MIC (minimum inhibitory concentration) of alcoholic green tea extract that inhibit Aggregatibacter actinomycetemcomitans growth was 60% and it was the same for Porphyromonous gingivalis.The MIC of aqueous green tea extract that inhibits Aggregatibacter actinomycetemcomitans growth was 70% and MIC of aqueous green tea extract extract that inhibits Porphyromonous gingivalis was 80%.The MBC (minimum bactericidal concentration) of alcoholic green tea extract that kills Aggregatibacter actinomycetemcomitans was 80%, the MBC of alcoholic green tea extract that kills Porphyromonous gingivalis was 90%, the MBC of aqueous green tea extract that kills Aggregatibacter actinomycetemcomitans growth was 90%, which was also the same for Porphyromonous gingivalis.HPLC analysis of aqueous and alcoholic green tea extracts that were used in this study revealed that alcoholic extract contained higher concentration of EGCG while aqueous extract had higher content of catechin and epicatechin.Conclusion : green tea extracts were effective against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, alcoholic green tea extract showed inhibition ability more than the aqueous green tea extract and more than chlorhexidine gluconate and it showed bactericidal activity at 80%, 90% and 100% concentrations.
