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دراسة مرضية وجزيئية لبكتريا Helicobacter pylori المعزولة من المرضى المصابين بالقرح المعدية والمعوية == Pathological and Molecular study on Helicobacter pylori isolated from patients with gastric and duodenal ulcers

Author name: رباب قاسم محمد الصكر
Supervisor name: ضحى سعد صالح | اياد محمد علي فاضل
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: جمعت80 خزعة نسيجية من المرضى المصابين بامراض القرح المعدية والمعوية، اظهرت نتائج الاختبارات النسيجية المرضية هيمنة التهاب المعدة المزمن بنسبة %35 واتت قرحة المعدة ثانية بنسبة %22.5.اظهرت الدراسة كفاءة اختبار اليوريزالسريع في التحري عن هذه الجرثومة اذ استطاع ان يثبت وجود هذه الجرثومة في 62 خزعة نسيجية واتت الاختبارات النسيجية المرضية ثانية حيث ظهرت جرثومة H.pylori في 47 خزعة نسيجية في حين تمكن الاستنبات البكتيري من الحصول على 42 عزلة بكتيرية.اثبت فحص الحساسية الدوائية تجاه عشر مضادات حيوية كفاءة المضادين الحيويين السيفوتاكسيم والريفامبسين في القضاء على هذه الجرثومة فقد كانت نسبة المقاومة لهما %10 واتى المضادان الحيويان التتراسايكلين والدوكسي سايكلين ثانية بنسبة مقاومة %20،%30 كانت نسبة المقاومة للارثرومايسين والكلارثرومايسين، فيما كانت نسبة المقاومة للاموكسيسلين %100 ونسبة المقاومة %80 لكل من المترونيدازول والسبروفلوكساسين اما الجنتامايسن فكانت نسبة المقاومة له %50.تم نمطت العزلات وراثيا باستعمال بوادىء متخصصة بالكشف عن قطعة الدنا التي تمثل المورث ure AB والمساوية الى 492زوج قاعدة لوحظ احتواء ثمان عزلات على هذا المورث فيما خلت العازلتان HP7 وHP9 من هذا المورث باستعمال تقنية الـ PCR.اظهرت نتائج النسق البلازميدي ان %50 من عزلات H.pylori تحوي بلازميدات، فقد احتوت العزلات (HP3, HP4, HP6, HP8) على بلازميدات صغيرة وامتلكت العزلتان (HP4,HP6) بلازميدا كبيرا فضلا عن بلازميدها الصغير فيما خلت العزلات الباقية من البلازميدات.اظهرت نتائج التحييد البلازميدي باستعمال صبغة بروميد الاثيديوم بوصفها مادة محيدة وبتركيز 3000 مايكوغرام/ مليليتر فقدان العزلة HP4 بلازميدها الكبير والصغير نتج عن ذلك فقدان تلك العزلة HP4صفة انتاج انزيم اليوريز وكذلك فقدانها المقاومة للمضادين الحيويين المترونيدازول Metronidazole والسروفلوكساسلين Ciprofloxacillin.اظهر التحول الوراثي Gentic transformation بين الدنا المستخلص من العزلة HP4 وبين العزلة HP1 التي عدت خلية كفوءة اكتساب الاخيرة صفة المقاومة للمترونيدازول اذ استطاعت ان تنمو على اوساط انتخابية تحوي 32 مايكوغرام/ مليلترمن هذا المضاد.اجريالتحول الوراثي ثانية بين دنا العزلة Hp4 وبكتريا تابعة لجنس اخر هي بكتريا Escherichia coli MM294 اظهرت نتائج هذه التجربة اكتساب بكتريا E.coli صفة انتاج انزيم اليوريزو المقاومة للمترونيدازول والسبروفلوكساسين من بكتريا H.pylori. | A 80 biopsies were collected from gastric & duodenal ulcers, showed dominance of chronic gastritis and gastric ulcers in aratio of %35 and %22.5, respectively.Rapid urease test, histopathological examination and cultural for detection of Helicobacter pylori, Showed the presence of this bacterium in 62 biopsies for urease test, in 47 biopsies for his topathological examination and in 42 biopsies in culture examination.Antibiotic susceptibility tests againt 10 isolates of H.pylori, Showed %10 resistance for cefotaxim & Rifampin, %20 resistance for Tetracyclin & Doxycyclin, %100 resistance for Amoxycillin, %80 resistance for Meteronidazole & ciprofloxacin and %50 resistance for Gentamicin.Genotyping by specific primer in PCR reaction for ureAB gene, Showed that 8 isolates were positive for this gene, where 2 isolates were negative.Plasmid profile for H.pylori isolatez Showed small single plasmid in each of HP3, HP4, HP6 and HP8 isolates and single small and larg plasmids for HP4 and HP6 isolates whereas other isolates were free of plasmids.Curing experiment for HP4 isolate by using ethidium bromide as acuring agent in aconcentration 3000 Mg/ ml, Showed the cured derivation lost their Urease production trait and resistance to meteronidazole & ciprofloxacin.Transformants resulted from transformation experiment between HP4 total DNA & HP1 as acompotent cell, Showed acquired ability to grow on selectve medium containing 32 Mg/ ml of metrronidazole.Another transformants, resulted from transformation experimeat between HP4 total DNA and Escherichia coli MM294 as acompetent cell, Showed acquired ability for Urease production & ciprofloxacin & Meteronidazole resistance.

انتاج وتنقية وتوصيف البلانتريسين من سلالات محلية لبكتريا Lactobacillus plantarum == Production, Purification and Characterization of Plantaricin from Local Strains of Lactobacillus plantarum

Author name: ولاء شوكت علي
Supervisor name: رشيد محجوب مصلح
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تم الحصول على 118 عزلة تعود لجنس عصيات الحليب Lactobacillus من الخضر المتخمرة والعجائن الحامضية، واخضعت جميع هذه العزلات للفحوصات الزرعية والمجهرية فضلا عن الفحوصات الكيموحيوية بغية تشخيصها حتى النوع واظهرت النتائج ان 73 عزلة منها تعود للنوع Lactobacillus plantarum ، منها 48 عزلة من الخضر المتخمرة و25 عزلة من العجائن الحامضية، وكانت هذه البكتريا سائدة في الخضر المتخمرة اذ بلغت نسبة عزلها 73.85% بينما بلغت نسبتها في العجائن الحامضية 47.17% . تم التحري عن قابلية عزلات بكترياLb.plantarum على انتاج البلانتريسين باستخدام 3 طرائق هي : الاشرطة المتقاطعة(flip - streak method) والتنقيط (spot on the lawn method) والانتشار بالحفر(well diffusion method) ضد 12 نوعا من البكتريا الموجبة والسالبة لصبغة غرام والمتضمنة : Staphylococcus aureus وStaphylococcus epidermidis وListeria monocytogenes وEnterococcus feacalis وLactobacillus acidophilusو Bacillus spp. وPseudomonas aeruginosa وPseudomonas fluorescens وSerratia marcescens وSalmonella typhi و. Klebsiella sppو Escherichia coli. واظهرت النتائج ان 8 عزلات كانت منتجة للبلانتريسين بطريقة flip - streak و7 عزلات كانت منتجة له بطريقة spot on the lawn بينما استطاعت 12 عزلة من انتاجه بطريقة الانتشار بالحفر،مما يدل على ان انتاج البلانتريسين في الاوساط السائلة كان افضل من انتاجه في الاوساط الصلبة،وعدت طريقة الانتشار بالحفر هي الافضل في التحري عن انتاج البلانتريسين. اظهرت البلانتريسينات المنتجة من عزلات Lb.plantarum فعالية عالية ضد P. aeruginosa عندما ابدت 83.3% من هذه العزلات فعاليتها التثبيطية ضد هذه البكتريا بينما اظهرت 16.7% فقط من هذه العزلات تاثيرها التثبيطي ضد بكتريا Lb. acidophilus ولم تبد اية عزلة من عزلات Lb.plantarum اية فعالية تثبيطية ضد بكتريا Bacillus spp. ، هذا وكانت العزلة Lb.plantarumVGW8 هي الافضل في انتاج البلانتريسين اذ تمكنت من انتاجه في الاوساط الصلبة والسائلة بالطرائق الثلاثة اعلاه، فضلا عن فعاليتها التثبيطية العالية تجاه 10 انواع من البكتريا الموجبة والسالبة لصبغة غرام. هذا وقد حددت الظروف المثلى لانتاج البلانتريسين من العزلة VGW8 Lb.plantarum واظهرت النتائج ان الوسط الامثل للانتاج كان الوسط المحضر مختبريا في هذه الدراسة والذي اطلق عليه وسط الانتاج السائل ورمز له (PLM) المتكون من 2% كلوكوز و2% ببتون و1.5% مستخلص الخميرة و1.5% مستخلص اللحم و0.25% من ملح K2HPO4 و0.5% من مادة tween 80 ،برقم هيدروجيني(pH) 5 وبدرجة حرارة30 م وبحجم لقاح 2% من المزروع البكتيري الذي يحتوي على 1×109 خلية /مل ولمدة حضانة 12 ساعة في ظروف لاهوائية. تمت في هذه الدراسة ايضا تنقية البلانتريسين اذ سخن المستخلص الخام للبلانتريسين بدرجة 80 م لمدة 10 دقائق قبل البدء بالتنقية والتي تضمنت خطوتين الاولى هي الاستخلاص بالمذيب العضوي (البيوتانول) والثانية الفصل بطريقة كروماتوغرافيا الترشيح الهلامي باستخدام عمود (Sepharose 6B). وقد اظهرت النتائج الحصول على البلانتريسين بفعالية نوعية 10666.67 وحدة/ملغم بروتين وبعدد مرات تنقية بلغ 13.067 وبحصيلة نهائية 12%.اطلق على البلانتريسين النقي اسم بلانتريسين VGW8 نسبة الى العزلة المنتجة Lb.plantarumVGW8 .كذلك فقد تم توصيف البلانتريسين المنقى VGW8 واظهرت النتائج ان وزنه الجزيئي كان 14400 دالتون بطريقة الترحيل الكهربائي بوجود المادة الماسخةsodium dodecyl sulfate(SDS) . واظهرت فعالية البلانتريسين النقي ثباتية عالية في قيم من الرقم الهيدروجيني(pH) تراوحت ما بين 3 الى 9 ،اذ احتفظ بكامل فعاليته عند تلك القيم لكنه فقد نصف فعاليته في pH( 2 و10) وكامل فعاليته في pH القاعدي (11 و12).كما اظهرالبلانتريسين ثباتية عالية تجاه المعاملات الحرارية المختلفة (20 - 100) م لمدد زمنية مختلفة شملت (10 و30 و60) دقيقة ويلاحظ من النتائج انه في الوقت الذي تمكن فيه البلانتريسين من الاحتفاظ بكامل فعاليته عند المعاملات الحرارية المختلفة (20 - 80 ) م للمدد الزمنية اعلاه، لم يتمكن من استرداد سوى نصف الفعالية عند تعرضه لدرجة حرارة 100 م لمدة 60 دقيقة او عند تعرضه لدرجة حرارة المؤصدة (121 م) لمدة 15 دقيقة. كما بينت النتائج طبيعة البلانتريسين البروتينية عندما فقد كامل فعاليته بعد معاملته بالانزيمات الحالة للبروتين ( ببسين وتربسين وبابين) في حين لم تتاثر فعاليته عند معاملته بالانزيمات (لايبيز والفا - اميليز ولايسوزايم).كذلك فقد اظهر البلانتريسين ثباتية عالية بعد معاملته بالمذيبات العضوية(كلوروفورم واسيتونايترل وداياثيل ايثر وايثانول وميثانول وايزوبروبانول وتولوين واثيل اسيتيت)، والمنظفات (توين80 وتوين20 وترايتون اكس100و EDTA ويورياو SDS) ، وبعض الايونات الفلزية (الصوديوم والبوتاسيوم والكاليسيوم والباريوم والزنك والحديد) حيث استعاد كامل فعاليته بعد معاملته بها لكن فعاليته انخفضت الى النصف بوجود ايوني المغنيسيوم والمنغنيز.تمت ايضا دراسة فعالية البلانتريسين VGW8 ضد P.aeruginosa وS.aureus واظهرت النتائج ان البلانتريسين يمتلك الفعالية القاتلة للبكتريا عندما انخفضت اعداد البكتريا مع زيادة تركيز البلانتريسين الى 320 وحدة /مل، فقد اظهرت النتائج ان هذا التركيز كان كافيا لقتل بكتريا P.aeruginosa بعد ساعتين من التعرض للبلانتريسين ولقتل بكتريا S.aureus بعد اربع ساعات من التعرض له. ايضا فقد تمت دراسة الفعالية التحللية للبلانتريسين VGW8 واوضحت النتائج انه يمتلك الفعالية الحالة للغشاء الخلوي عندما انخفضت الامتصاصية الضوئية عند الطول الموجي 600 نانوميترمن 0.59 الى 0.17 لبكتريا P.aeruginosa بعد ساعتين فقط من التعرض للبلانتريسين وبتركيز 320 وحدة/مل ، ومن 0.51 الى 0.20 لبكتريا S.aureus بعد 4 ساعات من التعرض للبلانتريسين وبالتركيز نفسه(320 وحدة/مل).وتمت ايضا دراسة تاثير البلانتريسين VGW8 على نفاذية الخلية وبينت النتائج ان الامتصاصية الضوئية عند الطول الموجي 260 نانوميتر قد سجلت زيادة من 0.5213 الى 2.9112 لبكتريا P.aeruginosa ومن0.4614 الى 2.6405 لبكتريا S.aureusدليلا على خروج الحامض النووي (DNA) من الخلايا نتيجة لتحللها.يتضح من هذه النتائج ان هدف البلانتريسين VGW8 هو الغشاء الخلوي. | A total of 118 isolates of Lactobacillus were obtained from fermented vegetables and sourdoughs. All the isolates were subjected to the cultural, microscopical and biochemical examinations for the identification of species. The results showed that 73 isolates belongs to Lactobacillus plantarum, 48 isolates from fermented vegetables and 25 isolates from sourdoughs. Lb. plantarum was dominate in fermented vegetables with 73.85% percentage of isolation while in sourdoughs the percentage of isolation was 47.17%.Three methods (flip - streak, spot on the lawn and agar well diffusion methods) were used to detect of plantaricin production by Lb. plantarum isolates against 12 species of Gram positive and Gram negative bacteria which included, Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Enterococcus feacalis, Lactobacillus acidophilus, Bacillus spp., Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens and Klebsiella spp. The results showed that 8 isolates produced plantaricin by flip - streak method, 7 isolates produced it by spot on the lawn method, while by the agar well diffusion method ,12 isolates were able to produce plantaricin, suggesting that the production of plantaricin was best in the broth medium than in the solid medium. The agar well diffusion method was considered the best one for detection of plantaricin production. Plantaricins of Lb. plantarum isolates exhibited high activity against P. aeruginosa when 83.3% of Lb. plantarum isolates showed antibacterial activity against this bacterium, whereas the lowest activity was against Lb. acidophilus when 16.7% of Lb. plantarum isolates showed antibacterial activity against it. No activity against Bacillus spp. was observed.Lb. plantarum VGW8, was the best isolate for plantaricin production. It produced it in both broth and solid media by all detection methods used in this study, this plantaricin exhibited a broad spectrum of activity against 10 species of both Gram positive and Gram negative bacteria. The optimum conditions for plantaricin production by Lb. plantarum VGW8 were determined and the results showed that the best production medium was production liquid medium (PLM) that prepared in this study, it composed of 2% glucose, 2% peptone, 1.5% yeast extract, 1.5% beef extract, 0.25% K2HPO4 and 0.5% tween 80,with pH 5, temperature 30°C for 12 hours under anaerobic conditions and inoculum size of 2% of cells number 1×109cell/ml.Purification of plantaricinVGW8 was made by heating crude plantaricin at 80ºC/10min and then purified by two steps method including extraction with n - butanol followed by gel filtration chromatography on Sepharose 6B column, the results showed that the specific activity was 10666.67AU/mg protein with 13.067purification folds and 12% recovery yield. The purified plantaricin was called plantaricin VGW8 according to the producer isolate Lb. plantarum VGW8.Physical characteristics were also studied and the results showed that the molecular weight of plantaricin VGW8 was 14400 Dalton by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE). Plantaricin VGW8 activity was stable at pH values (3 - 9) but 50% of its activity was lost at (2 and 10) pH values, and whole activity was lost at extreme alkali pH values(11 and 12).Also, plantaricin VGW8 showed high thermostability at different temperatures (20 - 100)°C for (10,30 and 60)min, it remained active after being treated with (20 - 80)°C for the periods above, but it retained only 50% of its activity after treatment at 100°C for one hour and autoclaving treatment (121°C/ 15 min).The results showed that the activity of plantaricin VGW8 disappeared when it treated with proteolytic enzymes (pepsin, trypsin and papain), whereas it retained whole activity when treated with lipase, lysozyme and α - amylase, indicating pure proteinaceous nature of purified plantaricin.As well as, treatment of plantaricin VGW8 with organic solvents (chloroform, acetonitrile, diethyl ether, ethanol, methanol, isopropanol, toluene and ethyl acetate), surfactants (tween 80, tween 20, triton X - 100, SDS, EDTA and urea) and the metal ions : Na, K, Ca, Ba, Zn and Fe, showed no effect of these treatments on the activity of plantaricin VGW8.While treatment with Mn and Mg reduced its activity to the half.The antibacterial activity of plantaricin VGW8 was studied against P. aeruginosa and S.aureus. It was observed that plantaricin VGW8 has bactericidal effect when the numbers of cells were decreased with increasing of plantaricin VGW8 concentration to 320 AU/ml. The results showed that the complete killing of P. aeruginosa was observed after 2 hours of incubation with 320 AU/ml of plantaricin VGW8, while the same effect was observed after 4 hours on S.aureus. Also, the lytic activity of plantaricin VGW8 was studied, and the results showed that exposure P. aeruginosa and S.aureus to 320 AU/ml of plantaricin VGW8 led to decrease absorbance at 600nm from 0.59 to 0.17 for P. aeruginosa within 2 hours and from 0.51 to 0.20 for S.aureus within 4 hours. Moreover, the effect of plantaricin VGW8 on the cell permeability was studied and the results showed that absorbance at 260nm increased from 0.5213 to 2.9112 for P. aeruginosa and from 0.4614 to 2.6405 for S.aureus, indicating that DNA leaked from cells due to the cell lysis. This means that the cell membrane is the target of plantaricin VGW8.

تاثير الذيفانات المعوية للبكتريا ETEC Escherichia coli في الخلايا السرطانية والخطوط الخلوية وفي حيونات التجارب == Effect of ETEC Escherichia coli enterotoxins on cancer cells, cell lines and laboratory animals

Author name: الهام سعيد عبد الكريم بنو
Supervisor name: رشيد محجوب مصلح | ناهي يوسف ياسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: his study aimed investigating the cytotoxic effect of crude enterotoxins on normal and cancer cells both invitro and invivo. the present study included isolation and identification of Pathogenic Escherichia coli, From Children (under 3 - years for both sexes) infected with severe diarrhea, from March to June 2005, and as follows : *Sixtysix Islates (60%) were obtained from 110 samples. These Isolates were Identified according to morphological and biochemical tests, and for confirmation by Api - 20E System. *Serological identification for these Isolates( 66 isoleates )showed that only 12 (18%) isolates were belonged to Enteropathogenic group of E. coli.(EPEC).* The results of using Suckling Mouse Assay(SMA) showed that only 13 (19.6%) out of 66 Isolates were capable of producing heat - stable (ST) enterotoxin, therefore these isolates belonged to Enerotoxigenic group of E.coli ( ETEC).Whereas the EPEC isolates were all negative for this test. According to the toxin activily that was evaluated by (SMA) the Isolate No. 99 determined as the most efficient Isolate in producing the (ST). The same Isolate (99) of E. coli showed its ability to produce heat Labile enterotoxin (LT), by using Rabbit lileal Loops.The Isolate (99) revealed for sensitivity to Ampicillin, Gentamicin, Nalidixic acid and Nitrofurantoin antibiotics, but resistance to Amoxicillin , Cefixime, Cephallothin, Ciprofloxacin and trimethoprim.This Isolate also showed its ability to adhere by using the special media that contain the congo red stain, and by using hemagglutination test because it posseses the colonization factors antigen (CFA/1) and CFA /III However it failed in producing hemolysin enzyme that hemolyze red blood cells The toxin reserved Its activity at temperatures (20,40,60 and 80)c0 , but loss some of its effect at 100 c0 and kept its activity at 4 c0 for (24 - 48) hrs, It was found that the highly toxic activity reduced in the PH 5,9.5. The time ofmice response to enterotoxin was determined. At 90 minutes, as the maximum of response and 180 minutes as the optimum time of response. Enterotoxin was purified partially by using sepharose CL - 6B, and the molecular weight for (ST) was 17378 dalton. LD50 of both bacterial Suspension and crude enterotoxin in mice was 2.13 × 108 cell/ mice, and 48.75 mg/ mice respectively. The crude enterotoxin showed more severe effect than the bacterial suspension in spleen, Intestine, and Lung, while the effect of bacteria suspension was more severe in Liver.The therapeutic dose of crude enterotoxin was determined according to LD50 in mice it was revealed that the concentration of 390 mg/kg have the activity in reducing the tumor volume when injected directly in tumor, with an inhibition ratio between(83 - 89)% beginning at 8th day of the 25th injecting days. While when the toxin was injected intraperitonealy, the inhibition ratio of tumor was Less than the injection in tumor it self. The dose 97.5 mg/kg that given daily for 25 days showed more efficiectly in reducing the tumor in percent of 73.3. The Comparitive study between the relative volume of tumor in treated group and the relative volume of tumor in control group revealed that there was significant difference statistically important all over the treatment time.. The necrosis and fibrosis were the most important characteristics in treated groups after histopathological examination which appearantly with the progress of treatment associated with volume decrease of tumor, so it was found that the last stage of treatment showed the cancer cells presented like small Land Surrounded by dense fibrous tissue. The treatment by using all toxin concentration in both ways of injections on murine bone marrow cells, showed significant increase in blast Index (BI) and mitotic Index (MI) when compared with control.The toxic effect of crude extraction was studied in tumor cell lines (in vitro), Hep - 2 and AMN - 3 and the normal cell line REF, the study showed that the toxic effect depend on the type of cells, the dose and the time of exposure. This study revealed that the AMN - 3 Cells were more Susceptible from that of Hep - 2 Cells and the high concentration caused inhibition to the growth of the tumor cells, Specially the concentration of 60000 Mg/ml, Also growth and multiplication of REF cells. Whereas the concentrations of 1875and 3750 Mg/ml were found as an inhibitor to REF Cells. Partially purified enterotoxin (ST) showed that it's effect was found to in hibit Hep - 2, AMN - 3 and REF cells at 72 hrs of exposure and has an inducer effect to growth of cells at 24 hrs of exposure in all concentrations,but the effect was differ in the time of exposure at 48hrs,that the three concentrations (1.986,3.965and 7.95) Mg/ml showed inducing effect ,while the three hight concentrations(15.86,158.6 and1586) Mg/ml showed inhibition effect to AMN - 3 cells.Also the hight concentrations(30000 and 60000) Mg/ml werefound as inhibitor to REF cells at( 48,72)hrs,but they were inducer of growth at 24hrs of exposure. The toxicity effects of crude enterotoxin were studied in human Lymphocyte multiplication (in vitro) , and the higher concencentration showed decline in Mitotic index (MI), but It was induced cells to transform in present of mitogen, so there was inverse in blast index (BI) when compare with the control.

دور الببتيدو كلايكان في امراضية بكتريا Staphylococcus saprophyticus == Role of Peptidoglycan in Pathogenicity of Staphylococcus saprophyticus

Author name: عذراء صالح فليح الهيتي
Supervisor name: مي طالب فليح | حارث جبار فهد
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: من مجموع 300 عينة ادرار تم الحصول على 71 عزلة عائدة لجنس الـ Staphylococcus من مريضات مصابات بخمج المجاري البولية واظهر عائدية 16 عزلة للنوع S. saprophyticus تم تشخيصها بوساطة الاختبارات الكيموحيوية ، كما تم اختبار حساسيتها للمضادات الحيوية ، استخدمت عملية التكسير الميكانيكي (Mechanical disintegration) باستخدام المازج (vortex) والحبيبات الزجاجية ، والتكسير باستخدام جهاز الذبذبات فوق الصوتية لتكسير جدار خلية العزلةS67 saprophyticus S. والحصول على خلايا متكسرة تمثل الجدار الخلوي الخام ومن ثم استخلاص وتنقية جزئية للببتيدوكلايكان بالاعتماد على الهضم الانزيمي بوساطة انزيمات الـ DNase وRNase وPronase وTrypsin فضلا عن استخدام مركب سلفات دودسيل الصوديوم (SDS) ومواد كيميائية اخرى ؛لازالة الاغشية السايتوبلازمية والمحتويات البروتينية الاخرى ؛ وبذلك تم الحصول على ببتيدوكلايكان نقي خال من الشوائب وهذا ما اكده الفحص بالمجهر الضوئي والالكتروني وقد اظهر الببتيدوكلايكان حزمة بروتينية واحدة عند اجراء الرحلان الكهربائي بهلام متعدد الاكريل امايد تحت ظروف غير ماسخة مقارنة بالجدار الخلوي الخام والذي اظهر عدة حزم بروتينية ؛ مما يدل على كفاءة الطريقة المستخدمة في الاستخلاص. تضمن البحث دراسة امراضية بكترياS67 S. saprophyticus المسببة لخمج المجاري البولية في الفئران اذ حقنت مجموعة من الفئران الاناث البيض عبر الاحليل وباستخدام القثطرة بتركيز 109 خلية جرثومية / مليلتر ، وحقنت مجموعة اخرى من الفئران بتركيز 3 ملغم / 0.2 ملليتر من الببتيدوكلايكان المستخلص منS67 S. saprophyticus والمنقى جزئيا وتم تشريح الفئران حيث اظهرت الاعضاء (الكلى والمثانة) تغييرات نسيجية ممرضة تمثلت بانكماش الكبيبة الكلوية واحتقان الاوعية الدموية للكلية وارتشاح الخلايا الالتهابية وفقدان طبقة الكيراتين في المثانة سواء اكانت محقونة بالجراثيم ام بالببتيدوكلايكان . حضر المصل المضاد للببتيدوكلايكان في الحيوانات المختبرية (الارانب البيض) وتبين ان الببتيدوكلايكان ممنع جيد عند حقنه في الارانب ، وتم اثبات وجود الاجسام المضادة للببتيدوكلايكان باستخدام اختبار الندف بالانابيب واختبار التلازن الجرثومي (Bacterial agglutination) حيث كون المصل المضاد للببتيدوكلايكان المعزول من جدار بكتريا S.saprophyticusS67 تلازنا مع جراثيم البكتريا نفسها وايضا اعطى تلازنا مع العزلة القياسية ATCC6538 S. aureusمما يشير الى وجود تفاعل تصالبي لهذه الاضداد. تم دراسة التاثيرات السريرية للببتيدوكلايكان في الارانب المحقونة، اذ لوحظ ارتفاع في مستوى اليوريا والكرياتنين وانزيمي GPT وGOT والكلوكوز في الدم وانخفاض مستوى انزيم الفوسفتيز القاعدي( Alkaline phosphatase ) مما قد يدل على وجود خلل وظيفي في عدد من الاعضاء مثل : الكلى ،و الكبد ،و البنكرياس نتيجة تاثرها بالببتيدوكلايكان | Seventy one isolates of Staphylococcus were obtained from 300 urine samples isolated from female patients with urinary tract infection. sixteen of these isolates were identified as S.saprophyticus by biochemical tests , the sensitivity of the isolates to different antibiotics were also studiedMechanical disintegration method using vortex and glass beeds in addition of Ultrasonicator were used for preparing disintegrated cell wall which represent Crude cell wall , Extraction and partial purification of the peptidoglycan were accomplished by enzymatic digestion using DNase ,RNase ,Pronase and Trypsin in addition to SDS and other chemicals to remove the cytoplasm membrane and other protein contents, so we obtained pure peptidoglycan that were confirmed by light and electron microscope, the partial purified peptidoglycan showed one protein band compared with crude cell wall which showed many protein bands when performed in poly acryl amide gel electrophoresis under non denaturing conditions which improved the efficacy of method that employed in extraction of peptidoglycan The pathogenicity of the S.saprophyticus in urinary tract infection of mice was studied, Female mice were injected transurethrally by using catheter with 109 germ cells concentration /ml, other group of mice were injected by 3mg concentration /0.2ml of partial purified peptidoglycan which extracted from S.saprophyticus , The histopathological changes in the organs (Kidney and Bladder) whether it was injected with germ or the peptidoglycan were observed Antiserum of the peptidoglycan was prepared in the laboratory animals (white rabbit) the results revealed that the peptidoglycan was a good immunogen, The presence of antibodies was shown by tube flocculation test and bacterial agglutination test.Thiese antibodies cause agglutination with S.saprophyticus by using microtiter plate and also give cross - reaction with ATCC5538P S.aureusThe clinical effects of the peptidoglycan in the injected rabbit were also studied, The levels of Urea,Creatinine ,GOT, GPT ,Glucose were increased in the blood where the level of Alkine phosphatase was decreased which indicate that the peptidoglycan caused dysfunction of several organs like kidney,liver and bladder

التشخيص الجزيئي, المظهري واختبار حساسية الفطريات والبكتريا المرافقة لقشرة الشعر مختبريا لعوامل التضاد الميكروبي == Molecular, Phenotyping identification and invitro susceptibility testing in Fungi and Bacteria associated with hair dandruff to antimicrobial agents

Author name: شيماء محمد علي وتوت
Supervisor name: زيدان خليف عمران المعموري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Babylon - College Of Science For Girls
Language: English
University location: Babylon
First pages:
Abstract: تمثل قشرة الراس اكثر مرض جلدي شيوعا ورغم انها غير مهلكة للحياة فلم تنل قسطا من الاهتمام والبحث على مستوى العالم ولذلك هدفت الدراسة الحالية الى عزل, تشخيص والتحري عن تاثير المضادات الفطرية على الفطريات والخمائر وكذلك عزل وتشخيص البكتريا المرافقة للقشرة الموجودة في فروة الراس ومن شعر هذه الحالات المرضية. خلال مدة الدراسة ولفترة من تشرين الاول الى حزيران 2016 حيث تم جمع 280 عينة قشرة وشعر ومسح من الحالات الموصوفة انفا واشتملت على 152عينة شعر و74 عينة قشرة و54 مسحة من فروة الراس وكذلك جمعت 30 عينة سيطرة. وكل جهد لم يشار اليه بمصدر فهو من جهد الباحث.زرعت العينات على وسط اكار السابرويد والدكستروز(SDA) الحاوي على مضاد السايكلوهكسيمايد وبدونه وشخصت عزلات الخمائر اوليا على وسط CHROMagar للتميز بين انواع الخمائر عن طريق الوانها. كذلك تم التحري عن الفطريات الخيطية الجلدية من خلال زراعتها على وسط اكار السابرويد والدكستروز(SDA) الحاوي على مضاد السايكلوهكسومايد للحصول على عزلات للفطريات الجلدية وكذلك تقييم تواجد الفطريات المترممة مع العينات قيد الدراسة وتقيم عوامل الضراوة لدى الخمائر. تشخيص الفطريات المعزولة على المستوى الجزيئي من خلال تضخيم منطقة ITS باستخدام الزوج الباديئي ITS5/ITS4وITS3/ITS4 واجراء تحليل التتابع DNA sequencing لعينات ممثلة عن المجوعات المتماثلة في اوزانها الجزيئية لمنطقة التضخيم وبعد الحصول على نتائج التضخيم تم اخذ منتج بلمرة لعزلات ممثلة وارسلت الى مختبرMicrogene في امريكا للحصول على التتابع للقواعد النتروجينية ومقارنتها مع تتابع العينات المرجعية في البنك الجيني باستخدام برنامج NCBI )Blast Nucliotide Database). ومطابقة التتابعات لتقييم التماثل والاختلاف ورسم الشجرة التطورية على اساس نتائج تحليل التتابع لتقييم مدى تقارب العزلات ضمن النوع والانواع قيد الدراسة.اظهرت نتائج التشخيص التقليدية والجزيئية باستخدام الازواج البادئية ITS5/ITS4 وITS3/ITS4 كانت اوزان الحزم لكامل منطقة ITS تتراوح بين500 - 800 زوج قاعدي. بينما تراوحت الاوزان لمنطقة ITS2 بين350 - 400زوج قاعدي.واظهرت النتائج ان الطرق الجزيئية المتقدمة هي ادوات قوية لتشخيص الفطريات مقارنة مع الطرق التقليدية. تم تسجيل عزلتين من C. sake و3 عزلة Aurobasidium iranianum وعزلة Issatchenkia orientalis وعزلة من Cryptococcus fonsecae وعزلة واحدة من Cryptococcus diffluens لاول مرة في العراق.اجراء تقييم للفعالية المايكروبية لبعض المستخلصات النباتية المهمة طبيا ومقارنتها مع المضادات الفطرية وتقييم التركيز المثبط الادنى باستخدام شريط E - Test strips .كما تم مسح الانواع البكتيرية المرافقة للعينات قيد الدراسة مجهريا وبايوكيميائيا من خلال زراعتها على الاوساط التفريقية مثل وسط Manitol salt agar ووسط Blood agar base.اظهرت عزلات المبيضات البيضاء موجبة لانتاج الانبوبة الجرثومية, النمو بوجود السايكلوهكسيمايد والنمو في درجة ˚45م. قابلية C.kruzei , C.parapsilosi وC.glabrata على تكوين الغشاء الحيوي . اما اختبار الانزيمات الحالة للدهون المفسفرة فقد كانت موجبة لعزلات C.albicans ,C.parapisilosis وC.kruzei واخيرا اضهر اختبار الانزيمات الحالة للدهون نتيجة موجبة فقط لعزلات C.albicans ,C.tropicalis وC.parapsilosis. كذلك اجريت اختبارات للفطريات الجلدية والتي تضمنت قدرة ال T.rubrum على النمو على وسط SDA with 5% of NaCl وكذلك نمو الفطريات على وسط PDA وانتاج صبغة عكسية.اظهرت النتائج ان لبعض المستخلصات المائية للنباتات تاثير مثبط على نمو الفطريات الجلدية والخمائر وكان المستخلص المائي للقرنفل اكثر فعالية في تثبيط الفطريات الجلدية وبعض الخمائر وال Rhodotorulla وبفرق معنوي ≥ 0.05 يليه الشاي الاخضر والكالبتوز والدارسين والحنة وبتركيز 8 % حيث كان اكثر تاثير من بقية التراكيز.تم اجراء اختبار الحساسية الدوائية لبعض المضادات الفطرية باستخدام E - Test strips للمضادين CAS وFLU حيث اظهرت النتائج مقاومة الفطريات الجلدية والخمائر وال Rhodotorulla للمضادين.تم عزل بكتريا Staph.aurues and Staph.epidermidisوبين اختبار ال Congo red قدرتها على تكوين الغشاء الحيوي Biofilms)). واستنتجت الدراسة ان قشرة الشعر هي مشكلة الشعر المهمة العديد من الخمائر مع الشعر المتساقط معها قد تقف وراءها مجموعة من الفطريات والبكتيريا المتهمة في احداث مثل هكذا اخماج وان استخدام المستخلصات النباتية كانت مؤثرة بشكل معنوي في اختزال نمو الفطريات التي تم اختبارها تحت تاثير هذه النواتج الطبيعية وبغياب الفعالية الدوائية لافضل المضادات الفطرية مثل الفلوكنزول والكاسبوفنجين. ان التشخيص المظهري للعزلات اعتمادا على شكل الخلية لانواع المبيضات والوانها على وسط الكرومواكار هو غير علمي لنجاح التميز بين انواع المبيضات. لذلك تشخيص الانواع بالاعتماد على منطقة ITS يوفر وقتا (والذي ياخذ وقت من يوم الى يومين ) وهي ملائمة وتطبيقية حتى مع العزل التي تمتلك خصائص مظهرية شاذة. | Hair dandruff is a most commercially demoralized skin disease. Although it is not deadly disease, but not have more attention and research worldwide. This study was aimed for isolation, identification and investigation of antifungal susceptibility of dermatophytes and Candida also isolation and identification of bacteria associated with hairs dandruff. During the period from September to June a total of 280 sample of scalp and hair and smear were collected from Hilla hospital private clinics and primary schools in rural areas in the Province of Babylon (n=152 hair samples, n= 74 dandruff samples, n= 54 smear from head scalp) and samples was also collected as 30 control samples. Every effort not referred to source it from researcher effort. Samples cultured on Sabouroud Dextrose Agar (SDA) medium with or without Cyclohexemide based on stander cultured methods, incubation and identification, Primary identification of Candida has been achieved on CHROMagar medium to differential between Candida spp. based on colors. Also it was investigation about dermatophytes through cultivation on Sabouroud Dextrose Agar containing Cyclohexomide to get isolates of dermatophytes, as well as assess the presence of fungus saprophytic with samples under study.Virulence factors for the yeast were detected. Fungal identification based on amplification internal transcript spacer (ITS) region by using ITS5/ITS4, ITS3/ITS4 primers and subject the PCR product to sequence analysis (DNA sequence) for representative samples groups at Micro gene laboratory in US. Sequence analysis based on alignment with data base of reference strains in the gene bank (Blast Nucliotide Database (NCBI) program). phylogeny tree was construction on the basis of the results of the sequence data.Results showed conventional and molecular diagnosis by used ITS5/ITS4 and ITS3/ITS4 primer was band weight integration to ITS region range between 500 - 800 bp. while range weight for ITS2 region between 350 - 400 bp.The results showed that advanced molecular assays were powerful tools for fungal identification compare with the conventional methods. For the first time in Iraq Two isolates from C.sake and one isolate from Issatchenkia orientalis, also three isolates Aurobasidium iranianum and one isolates from Cryptococcus fonsecae and one isolates from Cryptococcus diffluens were recorded.Evaluating the effectiveness of microbial for some medically important plant extracts and compared with the antifungal and assess the minimum inhibitory concentration by used E - Test strips. As it has been cleared bacterial species accompanying to samples under study microscopically and biochemically during cultured on differential medium like manitol salt agar and blood agar base media.C.albicans isolates positive results for germ tube, growth in the presence of Cyclohexomide and growth in temperature 45C˚. C.kruzei, C.parapsilosis, C.glabrata ability composition of biofilms, while phospholipase assay was positive to C.albicans, C.parapisilosis and C.kruzei. Lipase assay was positive to C.albicans, C.tropicalis, C.parapsilosis. As well as conducted test for dermatophytes which include ability of Trichophyton rubrum on growth on SDA with NaCl of 5 % and dermatophytes growth on PDA and produce inverse dye. The results showed that aqueous extracts were reduced the growth of dermatophytes and Candida. The aqueous extract of Cloves more effective in inhibition of dermatophytes, Candida and Rhodotorulla at ≥ 0.05 followed by green tea and Eucalyptus, Cinnomomum, Henna. Moreover 8% concentration was more effective than other concentrations.Tested antifungal sensitivity for some Fluconazole and Caspofungin antifungal using E - Test strips. Showed that dermatophytes, Candida and Rhodotorulla was reveals resistance action against antifungals.Staph.aureus and Staph.epidermidis showed Congo red test and ability for biofilms formation.The study concluded that the hair dandruff is important hair problem, many yeast with the hair loss together may be behind them groups from fungi and bacteria accused in events such as typhoid and used of plant extracts was effected in signification in fungi growth that test under effect this natural products and the absence pharmaceutical activity to best antibiotic such as Fluconazole and Caspofungin. Morphologically identified isolates based on the cell shapes of Candida spp. and their color on CHROMagar isn’t feasible to successfully differentiate between members of the Candida spp. Therefore, identification of Candida and dermatophytes base on ITS region saves time (it takes 2 to 3 days) and is accurate and applicable even to isolates with atypical morphological features.

دراسة تجريبية وجزيئية للبكتريا المصاحبة لحالات الحروق في محافظة ميسان == Experimental and Molecular Study of the Bacteria associated with burns in Maysan Governorate

Author name: حسين علي دنانه البهادلي
Supervisor name: رحمن لعيبي جلاب | حيدر خميس شنان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: Burn injuries are considered as health problems that may sometimes lead to rapid death. Bacteria are considered as the most important causes of microbial infection associated with burns. In present study, 75 clinical samples were collection from patients with burns for the period from October 2015 to June 2016 from the lobby burns in Sadr Teaching Hospital in the Maysan Governorate, these burn swabs revealed 56 bacterial isolates, 41 (73.21%) were Gram - negative bacterial, distributed of (35.71%) Pseudomonas aeruginosa, (28.57%) Klebsella Pneuomonia, (3.57%) Escherichia coli ,Enterobacter (5.35%). On the other hand 6(10.71%)were Gram - positive bacterial. out of 75 burned swabs, 84% revealed a single pathogens and 16% shown a mixed bacteria. On the other hand, Antimicrobial susceptibility for the bacterial burn isolates revealed that P. aeruginosa was resistant to routine tested antibiotics, like Amoxicillin, Gentamycin and Tobramycin in 100%, While was resistance to Ciprofloxacin 85%, while most P. aeruginosa was sensitive to Ceftizmiden in 50%. Results also showed that Staph. aureus was resistant to Gentamycin, Tobramycin, and Levofloxacin in 50% respectively.Depending on the rate of injury burns microbial and resistance to antibiotics used, p.aeruginosa and Staph.aureus isolated from the burnswere chosen and study their role in the events of infection, experimental burns, and for this purpose has been the use Albino mouse male as a model for a pilot study for pathogenesis and progress associated with the infection of the disease bacterial burns. (33) male mice were divided into tow groups, the first group(24) mice were burned (deep dermal second degree) and infection by 0.2X108 cfu kg of elected isolates, the route of infection was subcutaneously and scratching within burned area. In the second group six were infected without burn, which was considered as positive control, while in the thirdgroup three mice were burned and injected by 0.2ml of normal saline and consider as negative control .the first group was subdivided into two subgroups (12) mices for each burn dominant pathogens, then divided into subgroups each of six mices and following up (3,7, days intervals post infection process). P.aeruginosa burned mice group, shown skin infection more progressive and more severity in comporning with group infected by Staph. aureus, In addition, they were significant increases in the body temperature of all infection burned mice group(p.value)compared with those pre - infection process. Reisolation of bacteria from infection mice organs revealed, that high percentages in experimental p.aeruginosa burned model comparison with Staph. aureus.Diagnosed bacterial isolates by API 20and Vitek compact system, in addition to molecular diagnostics using PCR technique for the detection of 16S rRNA gene, All isolates showed a positive result of this gene. The amplified 16S rRNA gene seguence was compared with the seguence in NCBI seguence database.the bacterial strain identified as P.aeruginosa EPSI. This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial identification; various bacterial habitats are surveyed to compile their species compositions.

عزل وتشخيص البكتريا المرضية مع ايلاء اهتمام خاص للبكترياStaphylococcus auerus المرافقة ل Cutaneous Leishmaniasis في محافظة ذي قار == Isolation and Identification Pathogenic Bacteri with Special Regard to Staphylococcus auerus Associate with Cutaneous leishmaniasis at Thi - Qar Province

Author name: رضاء الله مرتضى الاعظمي
Supervisor name: قاسم حسن وداعة
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: The present study is designed to isolated and identification the pathogenic bacteria associated with Cutaneous leishmaniasis. Cutaneous leishmaniasis (CL) is a parasitic disease characterized by single or numerous ulcerations. This was study performed in Iraqi in Shatra general hospital and Al - hussein teaching hospital in Thi - Qar province 2016 - 2017.Total of 370 samples of suspected patients were taken and after examining them using geimsa stain , the positive were 186 males and 126 females then sterilize the area surrounding for ulcer , all samples were taken to (swab transport media) ,culture to MacConkey agar and blood agar after 24 hr of incubation at 37C ,after diagnosed by biochemical testing then species determined by API system. The positive results on culture media 92/312 , males 62 (67.4%) , female 30 (32.6 %).The total bacteria isolated from the lesions were in males and female staph auerus 52 cases (56.5%), Escherichia coli 8 cases(8.6%) , Bacillus cereus 6 cases(6.5%) , Pseudomonas. aeruginesa 5 cases (5.4% ), Strep. Pyogenes 6 cases(6.5%) ,Strepto.agalactiae 3 cases(3.3%).While other that isolated in males only and did not appear in females were aerobic bacteria ,Listeria moncytogenes 3 cases(3.4%) , Actinobacteria .spp 2 cases(2.2%) ,Klebsiella. Pneumonia 2 cases (2.2%) , Rodococcus .equi 1 cases (1.1%) , and anaeroberia bacteria ,Peptococcus niger 2 cases (2.2%) ,Peptostreptococcus .spp 2 cases (2.2%) .Then find bacteria that more presence more with cutaneous Leishmaniasis ,were it was staph. aureus therefore it were objected study antibiotic resistant .The present study demonstrates that Staph. aureus XIV isolates were resistant to methicillin 50 (96.15%), penicillin 48 (92.30%) , Clindamycin 37 (71.15%), ciprofloxacin 30 (57.69%) , Gentamicin 30 (57.69%),Amoxicalave 29 (55.76%), Azithromycin 25 (48.07%) , Amoxicillin 22 (42.30%), Tetracycline 21 (40.38%), Rifampin 10 (19.23%) and Vancomycin all staph .auerus are sensitive to Vancomycin The all 52 Staph. aureus isolates were further examined using polymerase chain reaction (PCR) for detection of (16SrRNA ,mecA and PVI gene ). 16SrRNA gene which were responsible for diagnosis ,where all isolates were positive to was 52\52 (100%) , mecA gene which were responsible for methicillin resistance this was detected in 52\52 (100%) . of Staph. aureus isolates and a part of virulence factors PVL gene was detected in 46/52(88.46%) of Staph. aureus isolates which are responsible to kill the leukocytes of human and other animals.

التوصيف المظهري والجزيئي للانواع البكتيرية المصاحبة لحالات الحروق في محافظة ذي قار == Phenotypic and Genotypic Charactrization of Bacterial species Assocuated with burn infections in ThiQar province

Author name: عبير هادي فرهود الحبيب
Supervisor name: رحمن لعيبي جلاب
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: هدفت الدراسة الحالية الى تشخيص الانواع البكتيرية المصاحبة لحالات الحروق والكشف الجزيئي عن الموروثه 16SrRNA في الانواع البكتيرية المشخصة واستخدام تقنية DNA Sequencing . تناولت الدراسة الحالية فحص ومعاينة 107 مريضا تم اختيارهم بشكل عشوائي (ذكور واناث) تراوحت اعمارهم بين 1 - 45 سنة من الراقدين في مستشفى الحسين التعليمي للمدة من حزيران - 2015 تشرين الاول 2016 اذ اجري الفحص السريري من قبل الطبيب المختص وتشخيص الاصابة على انها حالة حروق مرضية ناتجة عن اسباب مختلفة. جمعت 107 عينة مسحات قطنية Cotton swabs وزرعت على وسط اكار المكونكي ((MacConkey agar واكار الدم ((Blood agar والاكار المغذي ((Nutrient agar. شخصت العزلات عن طريق الاختبارات المظهرية والبايوكيمائية وعامل التشخيص API20E ونظام الفايتك ( (Vitek 2compactفضلا عن التشخيص الجزيئي باستخدام جين.16S rRNA واظهرت نتائج الدراسة الحالية وجود 117 عزلة بكتيرية تفوقت فيها العزلات السالبة لصبغة كرام ((Gram negative بنسبة 93.16% على العزلات الموجبة لصبغة كرام (Gram positive)بنسبة 6,83 وكانت نسب توزيع العزلات البكتيرية المشخصة Pseudomonas aeroginosa 32.47% , Klabseilla pneumonia 21.36% واقل نسبــة عزل كانـت لبكتريا Staphylococcus epidermidis بنسبة عزل 1.7% , في حين كانت نسب العزلات البكتيرية التالية Acinetobacter baumannii , Escherishia coli , . Enterobacter claocae ,Staphylococcus aureus , Burkholderia cepacia, Pruteus mirabillis, , Pantoea agglomerans بنسبة عزل. 12.36% , 11.1% , 5.12% , % 5.12 , 3.41% , 2.56 على التوالي.تناولت الدراسة الحالية بعض الجوانب الوبائية للحالات المصابة التي شملت معلومات عن المرضى المصابين بالحروق التي تضمنت الجنس , العمر , موقع السكن ونوع الحرق. وبينت النتائج ان عدد الحالات المشخصة كاصابات حروق للذكور كانت بنسبة 42.9% بينما كانت نسبة اصابة الاناث 57.%, تم تقسم المرضى الى عدد من المجاميع حسب الفئات العمرية. اعلى نسبة اصابة كانت في الفئة العمرية 9 - 1سنة بنسبة 54.2% وهي الفئة الخاصة بالاطفال تليها الفئة العمرية erishi29 - 20 بنسبة 20.56% واقل نسبة اصابة كانت في الفئة العمرية 49 - 40 بنسبة 4.67% وكانت نسب الفئات العمرية الاتية 19 - 10 , 39 - 30 بنسبة 11.21 , 9.34 على التوالي. اشارت النتائج الى ان حالات الاصابات المشخصة توزعت بنسب متفاوتة فيما يتعلق بنوع الحرق اذ كانت اعلى نسبة اصابة بالحروق بواسطة الماء المغلي Boiled water 38% تليها اصابة الحرق بواسطة لهيب الغاز Fire by gas flame بنسبة 28.9% واقل نسبة اصابة كانت لحروق الصدمة الكهربائية بنسبة 4.67%.اما فيما يتعلق بطبيعة ومكان السكن فقد اظهرت الدراسة ان اعلى نسبة اصابة كانت بين الافراد الذين يسكنون المناطق الريفية بنسبة 84.1% في حين كانت اصابات المركز بنسبة 15.88% . وجدت الدراسة الحالية ان نسبة الاصابة بالحوادث غير المقصودة كانت 86.9% في حين كانت الاصابات المقصودة 13% , لوحظ في الدراسة الحالية ان اعلى الاصابات بالحروق كانت تحدث في فصل الشتاء بنسبة 75.7% مقارنة باصابات الحروق في فصل الصيف 24.29% .اما بالنسبة الى اختبار فحص الحساسية فقد بينت نتائج الدراسة الحالية ان اكثر المضادات الحيوية فعالية ضد كل من البكتريا السالبة لصبغة كرامBacteria Gram negative والموجبة لصبغة كرام Gram positive هي Ceftazidin , Ciprofloxacin,Amicacin .واظهرت الدراسة ان اكثر العزلات مقاومة هي بكتريا P.aeruginosa التي اظهرت مقاومة لكل المضادات الحيوية بنسب متفاوتة. اكدت نتائج الدراسة الجزيئية لتضخيم جين 16SrRNA ان كل العزلات اعطت نتيجة موجبة لهذا الجين وان حجم الجين لكل الانواع البكتيرية كان 1500pb كذلك اكدت النتائج ان الحصول على500Pb من DNA Sequencing للجين 16SrRNA كافية لتشخيص الانواع البكتيرية كلا على حدة . ايضا تم مقارنة جميــــع نتائج DNA Sequencing للانواع البكتيرية (Pseudomonas aeruginosa ,Escherishi coli, Enterobacter cloaca , Proteus mirabillis, , Klebsiella pneumonia Staphylococcus.heamolyticus ,) التي اكدت تشابه نسب تتابع القواعد النيتروجينية مع نسب القواعد النيتروجينية المخزونة في البنك الجيني باستخدام برنامج MEGA6 Moleculas Evolutionary Genetics Analysis)) اكدت نتائج الدراسة الحالية على وجود 21 سلاله جديدة قيد التسجيل واعطيت الرمز TQU1 الى TQU12. | The present study addressed the examination and preview the 107 patients. who were randomly selected (males and females) and who ranged in age from 1 - 45 years old and have fallen asleep at the Hussein Teaching Hospital of the, period from Jon 2015 to November 2016. where he conducted clinical examination by a specialist doctor and diagnosed as a case of burns satisfactory result for different reasons. specimens were cultured on appropriated culture MacConkey agar, Blood agar and Nutrient agar, diagnosed isolates by phenotypic tests, Biochemical test, diagnosis factor API20E system Vitek 2 compact, as well as to molecular diagnostics, During this study, In total 117 bacterial isolates.109 isolates were Gram - negative bacilli and 8 were Gram - positive cocci. he distribution of bacterial isolates diagnosed Pseudomonas aeroginosa 32.47%, Klabseilla pneumonia 21.36% and the lowest rate was to isolate the bacteria by isolating Staph.epedermidis 1.7%, while the following bacterial isolates Acinetobacter baumennii, Escherishia coli, Enterobacter claocae, Staphylococcus .aueus, Burkholderia cepesa, Pruteus mirabillsi, Pantoea agglomerans by isolating : 12 : 82% , 11.1% , 5.12% 5.12% 3.41% 2.56 respectively. The present study addressed some of the epidemiological aspect of infected cases burns,which included information on patients with with burns, which included sex, age, residence location (place), burning type. The results showed that (42.9%) isolates were males and (57.%) were female.The most frequent patient were belonged to the 0 - 9 years old (54.2%) which is for children. 29 - 20 followed by isolation of 22 20.56% and less isolating rate was in the 49 - 40 age group. Created results also cases of infections diagnosed were distributed to varying degrees with respect to the type of cremation, where the highest rate of injury burns Boiled water 38%, followed by the burning by flames gas Fire 28.9%,Fire by gasoline flames (19.6%),Hot liquids (8.41%) and Electrical Shock(4.67%). As for the nature and place of residence have study found that the highest proportion of cases were among individuals who live in rural areas increased by 90 injured 84.1% while the injuries the center has reached 17 cases of burn injury by 10.88% ,Frequency of the manners of burning based on intention or accidentally was as follow : (86.95) of patients were burned accidentally and (13%) were intenion. Present results showed that the most frequent burning (75.7%)was occurred during winter. The lowest frequency (24.2%)was belonged to summer .So the most common time of burning of present was during winter. As for the screening test sensitivity was shown the results of the current study that the most effective antibiotics against both Gram - negative bacilli bacteria and Gram - positive cocci were Ceftazidin, Ciprofloxacin, Amicacin while most of isolates showed high resistance to Ticarkcillin, Gentamicin, Impenem. Where the highest proportion of resistant bacteria P.aeruginosa which showed high resistance to all antibiotics in different proportions. It showed the results of the molecular study to amplify the gene 16SrRNA that all isolates gave a positive result and it's within the range 1400pb - 1500 pb. Also it has been compared to the results of all the DNA Squencing bacterial species (P.aeruginosa, E.coli, E.cloaca, A.baumennii, P.mirabili), which confirmed its similarity ratios nitrogenous bases relay with nitrogenous bases ratios stored in the gene bank

تنقية جزئية وتوصيف Biosurfactant من بكتريا Bacillus subtilis وتاثيرها على نمو بعض عزلات البكتريا والفطريات

Author name: غـفـران فـلـيـح عـبد الـحـسـن عـزيـز الجـبـوري
Supervisor name: فاطمة عبد الحسين مجبل التميمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Kufa - College Of Science
Language: English
University location: Najaf
First pages:
Abstract: تم الحصول على 102عزلة من العزلات البكتيرية من 40 عينة من عينات الترب الملوثة بالنفط من مختلف المناطق ورشة عمل السيارات، محطات وقود السيارات، مصافي النفط ولخمسة مناطق مختلفة في العراق . خضعت كل هذه العزلات الى الفحص المجهري والخصائص الزرعية ووجد ان 80 عزلة منها تنتمي الى جنس Bacillus ، هذه العزلات خضعت ايضا لاختبارات بايوكيميائية وشخصت بناءا على دراسة rDNA 16S من قبل مجموعتين من البرايمرات (andB16SF B16SR) و(1492R and 27 F) اذ وجد ان 51 عزلة منها تعود الى Bacillus subtilis . تم فحص جميع هذه العزلات من حيث قدرتها على انتاج biosurfactant عن طريق فحص النشاط انحلال الدم على وسط دم الاغنام حيث وجد ان جميع العزلات كانت من النوع تحلل الدم بيتا حيث تم فحصها اعتمادا على قياس الشد السطحي بعد زراعتها في Jacques medium اظهرت النتائج ان 46 عزلة من 51 عزلة كانت منتجة biosurfactant , وقد تم اختيار ثمانية منها تميزت بانتاجها لهذا المركب وهي (S1,S2,S3 S4,S5,S6,S7,S8) حيث كانت العزلة B. subtilis S4 هي الاكفا في الانتاج لذلك اختيرت في الدراسات اللاحقة . تم استخلاص Biosurfactant الخام من العزلات المختارة بواسطة وتنقيته جزئيا باستخدام طريقة Ion exchange chromatography باستخدام DEAE - Cellulose تلاها طريقة Gel filtration chromatography باستخدام Sepharose - 6B . تمت دراسة خصائص المركبات المنقاة واظهرت النتائج ان الوزن الجزيئي biosurfactant التي تنتجهاB.subtilis S4 تراوح بين (1036 - 1058دالتون) من خلال جهاز HPLC - TOF - MS . اظهرت النتائج ان الحد الاقصى لانتاج biosurfactant من العزلة تم بطريقة استخدام زيت الزيتون (3٪) وكبريتات الامونيوم (0.3٪) وKH2PO4 (1.5) غم / لتر، وبدرجة حرارة 30 مº والاس الهايدروجيني (7) وفترة الحضانة لمدة 72 ساعة. وقد تحققت اعلى نسبة للـ biosurfactant بعد مكملات الظروف المثلى الى 0.3 غرام / لتر من الوسط الزرعي . تم اختبارالنشاط البكتيري والفطري للعزلات الثمانية ضد بعض الجراثيم المسببة للامراض، وهي Staphylococcus aureus,Streptococcus pneumonia, Pseudomonas aeruginosa ,Escherichia coli ,Proteus mirabilis, Candida albicans , Candida tropicalis , Candida krusei , Rhizopus oryzae , Aspergillus niger وكانت العزلة الاكثر فعالية هي B.subtilis S4 التي اظهرت وجود نشاط ضد كل مسببات الامراض ماعدا S.pneumonia ,P.aeruginosa ,C.krusei . كما بينت النتائج ان مستخلص العزلة B.subtilis S4 اظهر اعلى تثبيط نموعند التركيز 1500 مايكروغرام / مل وصل الى 24 ملم ضد E.coli، 23 ملم ضد K.aerogenes، 21 ملم ضد S.aureus، 15 ملم ضدC.albicans، 14 ملم ضد P.mirabilis، 11 ملم ضد C.tropicalis، وتثبيط نمو الفطريات، حيث وصل معدل النمو الشعاعي الى 31.9 ملم ضد A.niger و9.7 ملم ضد R.oryzae عند التركيز 2 ملغم / مل. كذلك اظهرت النتائج ان سمية المستخلص B.subtilis خارج الجسم الحي على ذكور الجرذان البيض بلغت حوالي 922 ملغم / كغم وتشير اذ ان الاعطاء اليومي biosurfactant لم تظهراي حالة وفاة في اي جرعة ، ولم تلاحظ اي تغييرات غير عادية في السلوك اوتاثيرات سمية خلال فترة 28 يوما من العلاج. واثبت التحاليل انه ليس هناك اي تاثير سلبي على معايير الدم الفسلجية وهذا يؤكد صحة هذه المستحضرات الحيوية للاستخدام . | About 102 bacterial isolates were obtained from 40 oil contaminated soil samples from various automobile work shop ,cars fuel stations ,oil refineries of five different areas in Iraq. Microscopic examination and cultural characteristics for all isolated were investigated , 80 isolates were found to be related to the genus Bacillus , Bacillus isolates were subjected to the biochemical tests and molecular identification based on the 16S rDNA by two sets of primers (B16SF and B16SR) , (27F and 1492R) of these 80 isolates revealed that 51 of the identified isolates belonged to Bacillus subtilis.All these isolates were screened for their ability for biosurfactant production through blood haemolysis activity on sheep blood agar indicated that all isolates were β - hemolysis while screening depended on measuring the lowering surface tension of cell free supernatant after cultivation in jacques medium which referred that 46 of 51 isolates were biosurfactant producers. Eight isolates were selected for the best biosurfactant producer isolatesCrude biosurfactants produced by the selected isolates were extracted by acid precipitation followed by partially purified with dichloromethane followed by purification and characteristics, the results showed that the molecular weight of biosurfactant produced by B.subtilis S4 ranging between 1036 - 1058 Da . Optimum conditions for biosurfactants production from B. subtilis S4 were determined. Results showed that maximum biosurfactant production from this isolate was achieved by using 3% olive oil , 0.3 % ammonium sulfate and 1.5 g / l Potassium dihydrogen phosphate ,at pH7,30 ºC and incubation period for 72 hrs. Maximum biosurfactant production was achieved after the supplementation of the optimum conditions was 0.3 g/l of culture medium .All of the eight isolates were tested for antimicrobial activity against twelve pathogenic microorganisms : Staphylococcus aureus ,Streptococcus pneumonia,Pseudomonas aeruginosa ,Escherichia coli ,Proteus mirabilis Klebsiella aerogenes,Candida albicans,C.tropicalis,C. krusei , Rhizopus oryzae , Aspergillus niger and Penicillium notatum.The most effective isolates were B.subtilis S4 which showed antimicrobial activity against some tested pathogens except S.pneumonia ,P. aeruginosa ,C.krusei and P.notatum.The results indicated that S4 culture extract showed high growth inhibition zone in concentration 1500 µg /ml reached to 24 , 23, 21, 15, 14 and 11 mm against E.coli , K.aerogenes , S.aureus , C.albicans, P.mirabilis,C.tropicalis respectively and high reduction in of radial growth of the fungi reached to 31.9 and 9.7 mm in A.niger and R.oryzae respectively at 2 mg /ml concentration .Study in vivo potential toxicity of B.subtilis lipopeptide biosurfactant towards male rat was evaluated. An LD50 value was determined to be about 922 mg / kg. Results show that daily administration of biosurfactant did not show any death cases at any dose. Also, no unusual changes in behavior and no intoxication were observed during the 28 days period of treatment. Analysis proved that there were no significant adverse effect on hematological parameters and this confirms the validity of those biopharmaceuticals on body of mammals.

دراسة لتكوين الغشاء الحيوي لبكتريا Escherichia coli وProteus mirabilis المعزلة من اصابات المسالك البولية في محافظة النجف الاشرف == Study of Biofilm formation of Escherichia coli and Proteus mirabilis isolated from urinary tract infection in AL - Najaf Al - Ashraf governorate

Author name: رسل عيدان محسن
Supervisor name: سهام جاسم محسن
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Pathological Analyzes
Language: Arabic
University location: Najaf
First pages:
Abstract: The aim of this study is to inferring the ability of biofilm production and virulence factors which has helped to form it into two types of bacteria P.mirabilis and E.coli. Two hundred and fifty samples have been collected from patients who are suffering Urinary tract infection (UTI) in Teaching Hospital at Al - Najaf Al - Ashraf during the period ( 1/10/2014 - 1/1/ 2015). The samples have has identified by diagnostic methods : culturing , microscopic , biochemical and using VITEK - 2 compact system more sensitively and specifically method.The results have been showed that the isolation ratio was 30 (37.5%) of E.coli, while P.mirabilis was 14(17.5%) also, a five isolates (6.5%) wereEnterobacter cloacae ssp cloacae and only two isolates (2.5%) were Raoultella ornithinolytica from the total number of specimens. The virulence factors and antibiotic sensitivity test of two bacteria : P.mirabilis and E.coli were determined and the more virulence and multidrug resistance were selected.The hemagglutination properties of P.mirabilis which isolated from clinical samples were determined , the reaction with red blood cells of human group O+ was explored. The ratio of Hemagglutination was reached 42.86%, 50%, 7.14% to MSHA, MRHA and NOHA respectively. However, E.coli has been a different results that was reached 30 % , 26.67% and 43.33% from type MSHA, MRHA and NOHA as well. On the other hand, the thin - film production (pellicle) has been formed by 13 isolates of bacteria P.mirabilis and 21 isolates of bacteria E.coli, when it was growth on the nutrient broth in a temperature 37˚C for 24 - 18 hours. Moreover, it has been shown that there is high affinity for adhesion with the epithelium cell in the urinary tract. In addition, the E.coli has proved the higher efficiency than P.mirabilis in adhesion. It was detected ability of bacteria to biofilm production in three methods. The bacteria E.coli have showed their ability on biofilm production where by the rate was (43.3, 52 and60)% on the different growth ways which was the Congo Red, the tube and the plate respectively.The gel electrophoresis has been done on all isolates which was to analyzed on adhesive factors genetically whereby the bacteria P.mirabilis, E.coli have shown ability to gene expression (fimH and afaB) with a rate of (42.86 and 28.57)% and (30 and 20)%, respectively.Also, it has been clarified that the antibiotics ( Ciprofloxacin, Amikacin and Ceftazidime) have an effect on the most virulent bacteria to prevent the formation of biofilm when it is taken a half of minimum inhibitory concentrations. It was found that the minimum inhibitory concentrations were the most efficient in the prevent a biofilm formation in both types of bacteria.It appears to be found that the minimum inhibitory concentrations of these antibiotics were the most efficient in the prevent the formation of biofilm in both types of bacteria. Ciprofloxacin has the high efficiency to prevent the biofilm formation in E.coli while this concentration has no effective on biofilm production from others. It has been found that there is a clear positive relationship between the presence of adhesion gene (fimH , afaB) and the ability to adhesion on the epithelial .The conjugation process was conducted successfully to transfer the adhesion genes between (E.coli and P.mirabilis) which have been considered a donor strains to transfer the genes to the standard strain E.coli HB101 which have been considered a receipt strains whereby have shown ability to adhesion and biofilm formation.

دراسة مسحية للبكتريا المرتبطة بالتهاب الزائدة الدودية وتحديد الحالة النسجية للمصابين في محافظة البصرة == Survey of bacteria associated with appendicitis and determination of Histological patient state in the province of Basrah

Author name: هدى عبد الرحيم مذكور
Supervisor name: محمد عبد الامام احمد | نوري حنون جاسم
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done to determined pathogenic bacteria that associated with appendicitis .This study includes ninety samples of removal appendix taken form patients who cleared diagnosed as appendicitis infection by specialised doctors in general Basrah hospital and Al - Sadir teaching hospital for the period between September - 2013 and June - 2014.Distribution of appendicitis appear in all age groups, 10 - 20 year and 20 - 30 year were the most infected, as well as the infection appear in both gender, but rate of infection in male was relatively higher than female 68(59%), 47(41%) respectively. The ratio of the city's population has overcome the rural population 96(83.5%),19 (16.5%) respectively. The percentage of samples that gave positive culture was 80( 88.9%), while 10 (11.1%) of these samples negative culture. The study reveals 15 different bacterial isolation, the most common bacteria was Escherichia coli 80(44.9%) while other species was appears in less percentage Shigella dysenteria 14(7.9%), Salmonella enterica typhi 10( 6.5 %), Pseudomonas aeruginosa 8( 5.6 %) , Klebseilla Pneumonia 6(4.3%) , Klebseilla oxytoca 3(1.7%), Morganella morganai 7 ( 9.3 %) Neisseria spp. 6 ( 9.5%) , Enterobacter cloaca 4 ( 2.2 %) ,Serreatia spp. 3 )7.1 %(, Citrobacter ferundii 2 (7.7%) Proteus vulgaris 7) 6.5 %( Staphylococcus spp. 14(1.3%), Streptococcus spp. 72(5.1 %) and Bacillus subtilis 8)5.6 %(. Laboratory diagnosis for blood samples included estimated of total WBCs and found that (31%) of patients have natural WBCs values while the other patients have high values.Antibiotic sensitivity test to E.coli isolates towards 20 antibiotics because she is the most common bacteria showed that all isolates were resisted for most antibiotics which used in test especially for β - lactam group, and the isolates of E.coli were multi resistance for antibiotics.Identified minimum inhibitory concentrations MIC for some antibiotics The results showed that E.coli bacterial isolates's 30 isolation have shown resistance to anti AMO as MIC values ranged from 128 - 1024 Mcgm / ml while the MIC for Anti AMP values between 4 - 128 Mcgm / ml, As for antibiotics and Cefixim , Ceftriaxone, Cefotaxime, and it was her MIC values ranging between (1 - 128) Mcgm / ml Plasmid profile of E.coli isolates investigated to study the correlation between plasmid profile and antibiotic resisitant marker and results from agarose gel electrophoresis revealed that all E.coli isolates contain one plasmid band.This study includes the detection of some genes that encode to beta - lactamase enzymes in E.coli which were responsible about multi antibiotic resistanc and these genes loaded on plasmid DNA for ten isolaes and found that 5(50%) from isolates have blaTEM gene and 5 )40%( have blaCTX gene and 1(10%) have blaSHV gene. This study also considered note the general appearance of appendix samples , some of them are enlarged and surrounded by vesicles, some with fibrous walls and ulcerated with mixed colors, then examined the histological changes it, the study showed changes in histological structure of the excess was extensively congestion of blood vessels, veins in serosa and subserosal layers and increased the amount of diffuse lymphoid tissue in the layers of the appendix walls.

دراسة وبائية وجزيئية للجرثومة Escherichia coli O157 : H7 المعزولة من عينات سريرية وبيئية في محافظة البصرة - العراق == Epidemiological and molecular study of the bacterium Escherichia coli O157 : H7 isolated from clinical and environmental samples Basra Governorate / Iraq

Author name: عباس ضرب شعبان
Supervisor name: ميثم ايوب عبد القادر الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 464 clinical and environmental samples attempt to isolate Escherechia coli O157 : H7 serotype were collected, of which 183 stool samples from diarrheal children at Al - Qurna hospital and Al - Medaina Hospital of children during the period between October 2013 - August 2014. Environmental samples (281) : 121 samples of drinking ( tap) water , 80 samples of river water , and 80 samples of sewage water (collected from different regions of Basra Governorate) . All samples was tested and detected the presence of Escherichia coli serotype O157 : H7 using chromogen agar and ( CT - SMA ) as selective media and using direct immunological testing for direct identification of the pathogen (Certeste) for clinical samples.The study revealed the isolation of 56 isolates of E.coli O157 : H7 at a rate of (12.06%) : 16 clinical isolates at a rate of (8.7%), 40 environmental isolates at a rate of (14.23) : 9 isolates from drinking water at a rate of (7.4%), 18 isolation from sewage at a rate of (22.5), and 13 river isolates at a rate of (16.25).Antibiotic susceptibility testing toward 20 antibiotic of E.coli O157 : H7 isolates showed that all isolates from all sources resistant to the antibiotics Ampicillin, Amoxacilllin, , Rifampin and Piperacillin while showed high sensitivity to antibiotics amikacin, Kanamcin, ciprofloxacin and norfloxacin.The clinical isolates of E.coli O157 : H7 showed multiple high antibiotic resistance index Muliple Antibiotics Resistance (MAR index) ranged between (0.25 - 0.8), Where two isolates showed a resistance to sixteen antibiotics, Two isolates showed resistance to twelve antibiotics, Two isolates showed a resistance to eleven antibiotics, one isolate showed a resistance to ten antibiotics,Three isolates showed a resistant to nine antibiotics and two isolates showed aresistance to eight antibiotics, One isolate showed a resistant to six antibiotics and two isolates showed resistance to five antibiotics.The environmental isolates of E.coli O157 : H7 showed less resistance ranged between (0.25 - 0.75) for samples of sewage and (0.35 - 0.50)rivers samples , and (0.30 - 0.55)tap water samples . As shown, one isolate resistant to fifteen antibiotics, one isolate showed a resistance to thirteen antibiotics and four isolates showed a resistance to ten antibiotics from sewage. As for rivers Tow isolates showed a resistance to ten antibiotics, three isolates showed a resistant to nine antibiotics while a one isolate of tap water showed a resistance to eleven antibiotics, three isolates showed a resistance to ten antibiotics and an one isolates showed a resistance to nine antibiotics and two isolates resistant to eight antibiotics.Investigating about the content of plasmid in isolates E.coli O157 : H7 to study the relationship between the plasmid content as an indicator of antibiotic resistance, as the results showed that most of the isolates E.coli O157 : H7 four clinical isolates containing the plasmid including one molecular weight > 10kbp with a Patterns of multiple different resistance,also two isolates contain three plasmids of a molecular weight > 10kb. And one isolate containing four plasmids with molecular weights ranging from (4 - 10Kb) and one isolate containing five plasmids resistant to sixteen antibiotics with molecule weights ranged between >(2 - 10Kb).The isolates E.coli O157 : H7 isolated from the environment , sixteen isolates showed that they contain plasmids of molecular weights> 10kb , one isolate contains two plasmids of molecular weights ranging between (4 - 10Kb), five isolates containing three plasmids of molecular weights ranging from (2.9 - 10) Kb and two isolates containing four plasmids of molecular weights ranging between( 2 - 10 Kb

انتاج وتوصيف متعدد السكريات الخارج خلوي Lactobacillus plantarum من بكتريا Exopolysaccharide المعزولة محليا ودراسة فعاليته البيولوجية == Production And Characterization of Exopolysaccharide (EPS) From Locally Isolated Lactobacillus Plantarum And Studying Its Biological Activity

Author name: بيداء مهدي عباس الغانمي
Supervisor name: ناجح هاشم كاظم الظويهري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kerbala - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Karbala
First pages:
Abstract: Twenty - five isolates of Bacillus spp. Were isolated from 15 soil samples collected from different places in the Holly city of Kerbala, 13 isolates lactobacillus spp. Were isolated from 15 milk products and 7 isolates pseudomonas spp. The isolates were screened for their ability to produce Exopolysaccharide.The results showed that isolate number 3 (Lactobacillus lac 3) was found to give the highest production of the polymer, the isolate has been identified as lactobacillus plantarum. The optimal cultural and environmental conditions for EPS production were been identified which include using the production medium (simplified synthetic medium) containing date juice (5%) as a carbon source and yeast extract (0.2%) as a nitrogen source, the media was enhanced with a total concentration 0.9% of magnesium sulphate and potassium phosphate as mineral salts. The primary pH was adjusted to 6.5 and the inoculum size was 3%(v/v), isolates were grown under anaerobic condition at 35 Co for 24 h and the EPS extracted with ethanol 95%. Two steps were used for partial purification of the polymer as follows, the first step by using TriChloroAcitic acid (TCA) solvent and the second step by dialysis. The yields were (77.4 and 63.8)% for the two purification steps respectively. The biological activity of the EPS extraction from L. plantarum has been tested against some species of G+ve and G - ve bacteria and some fungal. The results showed that EPS extrac exhibits an inhibitory activity against Stenotrophomonas, Bacillus cereus and Proteus bacteria with an inhibition zone diameter of 23, 21, 19 mm respectively. While, there was no inhibitory effect against fungi. Antioxidant activity has also been tested, where IC50 value against ABTS free radical at 100 Mg / ml. The characterization of partially purified EPS is investigated by using Fourier Transform Infrared Spectroscopy (F.T.I.R). The molecular weight of the polymer was also measured by using viscometer and the molecular weight obtained by this technique was 2.3 *105 Dalton. The EPS extraction from selected isolate showed two spots when separated by Thin Layer Chromatography (TLC) which is analyzed as a glucose and galacto

تحضير لقاح مضاد للبكتريا المرضية المسببة لالتهاب القــدم السكري باستخدام التشعيع بالليـزر واطئ الطاقة == Preparation of Vaccine Against Diabetic Foot Pathogenic Bacteria Using Low Level Diode Laser

Author name: زينب عواد راضي محمد
Supervisor name: احسان فتح الله رستم محمد
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: Al-Muthanna University - College Of Science - Department Of Biology
Language: English
University location: Muthanna
First pages:
Abstract: تهدف الدراسة الحالية الى تحضير نوعين من اللقاح (الحي المضعف، والمقتول) ضد البكتريا المسببة لحالات داء القدم السكري في الانسان، وذلك باستخدام اشعة الليزر ثنائي الصمام. تم جمع العينات من (40) شخص يعانون من التهاب القدم السكري، باستخدام مسحة قطنية معقمة Sw | The objective of this study is to prepare two types of vaccine (Live attenuated and killed vaccines) against pathogenic bacteria of diabetic foot infection in humans, using laser irradiation. Samples collected from forty patients suffered from diabetic foot infection, using sterile cotton tinge (Swab), the samples diagnosed depending on a number of morphological examinations, biochemical tests and culturing on selective media, as well as to use of the API system. The bacterial isolates obtained including the followings : Staphylococcus aureus (24.59 %), Klebsiella pneumoniae (22.95 %), Escherichia coli (21.31 %), S. epidermidis (9.84 %), Pseudomonas aeruginosa (9.84 %), Proteus mirabilis (8.20 %), and other species (3.28 %). The sensitivity of the bacterial isolates before irradiation to a number of antibiotics were examined, they all gave high resistance to the antibiotics, except two types (Amikacin & Ciprofloxacin), which the bacterial isolates were sensitive to. The bacterial isolates irradiated with laser using wavelengths (660, 820, and 915nm) to increase their sensitivity reaching to a step of attenuating or killing the bacteria with increasing exposure times. Then the attenuated and killed bacteria from each isolate used to prepare a mixed vaccine. Laser effect on the bacterial isolates showed a significant decrease in the viability of the bacteria of all species when the dose was increased, where occur killing the bacteria after 20 min and more of laser dose. The efficiency of the vaccine was tested by using laboratory animals, fifteen rabbits were used in the current study, they were divided into three groups with five rabbits each, one group for live attenuated vaccine inoculation, and the other one for the killed vaccine while the third group used as a control group. One month after the completion of the vaccination, the concentrations of the immunoglobulins (IgG, IgA, IgM C3, and C4) in the rabbit serum measured using Radial Immunodiffusion (RID) method.The results showed very high significant differences P < 0.001 for the level of IgG between the live attenuated vaccine group when compared with the control one, high significant differences P < 0.01 for the level of killed vaccine group compared with the control one.The results of IgA concentrations for the three groups were highly significant, P < 0.01, when comparing the attenuated with control group, while were significant, P < 0.05 between the killed vaccine group and the control one, it was also significant for the level of IgM, C3, and C4, when compared both the live attenuated and killed vaccine groups with the control one respectively. There were no significant differences between the live attenuated and the killed vaccine groups of all measured concentrations.Subsequently the animals inoculated again with a live dose of the bacterial isolates and the levels of (IgM, IgA, IgG, C3, and C4) were measured, the same results as in the vaccinations readings were obtained.Finally the animals were inoculated with the challenge dose of all the isolated live bacteria. The animals of the control group died, while the immunized animals remained healthy revealing the efficacy of the vaccine and the vaccination program.

انتشار بكتريا Escherichia coli في المرضى في المرضى المصابين بالتهاب المرارة الحصوي في محافظة الديوانية == Dissemination of Escherichia Coli In Patients With Cholecystitis Calculus In Al - Diwanyia Governorate

Author name: احمد رزاق واجد الداوودي
Supervisor name: سيوف خومان علوان الرماحي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al-Qadisiyah - College Of Science - Department Of Biology
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: The study aims to identify Escherichia coli bacteria, which causes calculus cholecystitis of molecular perspective. The study samples were gathered from 110 patients who undergone cholecystectomy in Diwaniya general hospital for the period October 2013 to April 2014. The sample included bile sap, gall bladder tissues and stone if available. The samples were cultured to identify their culture specifications.The results showed that chronic cholecystitis is the most common one, which has 55.5% then acute cholecystitis by 44.5%. The study results showed that age and sex have an influence on cholecystitis. The highest percentage of infection is the ages 36 - 45 for the two sexes. If the cases distributed according to the patient sex, there is a difference in cholecystitis between male and female, where females infections are 88.2% and males are 11.8%. The total isolated bacteria were 125 bacterial isolates of 52% of gall bladder tissues, 26.4% of bile and 21.6% of the stone. Escherichia coli had 34.4% of the total samples of 51.1%, 36.6% and 29.6% respectively of the above mentioned samples.As far as susceptibility for all Escherichia coli isolates towards 15 types of antibiotics by using disc diffusion for Kerby - Bauer method, bacteria had high susceptibility against ? - lactamases antibiotics where they had susceptibility against Ampicillin+ Clavulanic acid by 38.7% and 79% for Ampicillin. The least susceptibility was for Amoxicillin by 69.7%. Bacteria had no resistance for Imipenem where their susceptibility was 100%. Bacteria had weak susceptibility against Aztreonam by 13.9%. While with cephalosporins, susceptibility for Ceftazidime by 27.9% and for Cefotaxime by 20.9%. With Aminoglycosides, resistance to Gentamicin antibiotics was 34.8% and 30.2% for Amikacin. The results showed that resistance to Quinolones was 16.2 for Nalidixic acid while for fluoroquinolones resistance was 25.5% for Ciprofloxacin and 18.6% for Norfloxacin. Isolates ability to produce CTX - M, SHV, TEM and AMPC extended spectrum ? - lactamases enzymes was tested by investigating the existence of blaTem, blaAMC, blaCTX - M and blaSHV genes in these isolates through Polymerase chain reaction (PCR). There were 23/26 isolates (88.4%) showed their ability to contain blaAmpC gene, and 7/26 isolates (26.9%) contained blaSHV gene. There are 18/26 isolates(69.2%) contained blaTEM gene while 20/26 isolates (76.9%) contained blaCTX - M.Through using Quantitative Real Time - Polymerase chain Reaction (qRT - PCR), gene expression measurement for blaCTX - M, blaTEM and blaAmpC was measured by relative numbers. The results showed that gene expression measurement for ? - lactamases enzymes type AmpC produce by Escherichia coli was the highest if compared with ? - lactamases enzymes type TEM and CTX - M with different periods of incubation, which is T4h for 4 hours, T24h for 24 hours and T48h hours of incubation respectively. It is noticed that genes responsible for producing ? - lactamase enzymes type TEM, CTX - M and AmpC are changed during different periods of incubation through using Real Time - Polymerase chain Reaction (RT - PCR).

التحري عن عوامل الضراوة لبكتيريا اشيريشيا القولون المعزولة من المرضى المصابين بالتهاب المجاري البولية وقياس نمط بعض الحركيات الخلوية لديهم == Detection of Virulence Factors of Escherichia Coli Bacteria Which Isolated From Patient With Urinary Tract Infection And Measuring Some Cytokines In Them

Author name: رواء ماجد محمد البو صالح
Supervisor name: ميثم غالي يوسف
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al-Qadisiyah - College Of Science - Department Of Biology
Language: Arabic
Full text from the source: https://qu.edu.iq/repository/wp-content/uploads/2017/04/%D8%B1%D9%88%D8%A7%D8%A1-%D8%A7%D9%84%D8%A8%D9%88%D8%B5%D8%A7%D9%84%D8%AD.pdf
University location: Qadisiyah
First pages:
Abstract: تضمنت هذه الدراسة جمع 100 عينة من اعمار مختلفة للمرضى المصابين بالتهاب المجاري البولية الذين راجعوا مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية , خلال المدة من تشرين الثاني 2012 الى نيسان 2013 للتحري عن بكتيريا Escherichia coli ا | groups of patient with pyelonephritis, whose visited Al - Dewaniya teaching and Woman s and children hospital in Al - Dewaniya city during the period from November 2012 to April 2013 for detection the isolates of Escherichia coli. The results showed that 56% of bacterial isolates were E.coli and 44% isolates belonged to others bacterial types. These results indicated that the females were more infected 67.86%(38\56) with pyelonephritis than the males 32.41(18\56). Always our result were pointed that the age of 30 - 39 old years have beeninfected with pyelonephritis and present the high percentage infection 30.36% comparison with others age groups (1 - 9, 10 - 19, 20 - 29, 30 - 39, 40 - 49, 50 - 59, 60 - 69, 70 - 79) , with percentage of (1.79%, 7.14%, 14.29%, 30.36%, 19.64%, 10.71%, 12.50%, 3.57%) respectively. The resistance of E.coli isolates to the four generation of Cephalosporin antibiotics were showed the following state : - The first generation of Cephalosporin antibiotic were indicated to Cephalothin 76.6%, Cefazolin 71.4%, Cephlexin 69.7% and Cephadroxil 66.1%. - The second generation of Cephalosporin antibiotic were indicated to Cefaclor 59.0%, Cefonicid 60.8%, Cefprozil 50.0%, Cefoxitin 64.2% and Cefmetazole 55.3%. - The third generation of Cephalosporin antibiotic were indicated to Ceftriaxone 32.2%, Cefotaxim 35.7%, Ceftazidim 37.5%, Cefixim 42.9%, Cefdinir 35.7% and Ceftizoxim 30.4%. - The fourth generation of Cephalosporin antibiotic were consisting of one antibiotic only like Cefepime with percentage of 39.3% The results concerned the virulence factors genes showed that the gene irp2 witch responsible for taking the iron from the blood, whereas the gene pap responsible for the production of P - type Pilli, afa gene responsible for the production fimbriae, iha gene responsible for production of capsule and the gene tst responsible for toxic shock. The result of PCR for the E.coli DNA showed that all the thirty isolates of this bacteria contain the gene irp2, while the others genes pap, afa, hly, and iha were presented the following percentage 36.6%, 30.0%, 96.6% and 10.0%respectively, whereas the gene tst didn’t recorded any isolates concerting. So in this study was to measure immune factors related to the urinary tract infection is in general and pyelonephritis in specially, has been measure some of cytokines which (Interleukin - 8, Interleukin - 6, Tumor necrosis factor - ?), they found increase in these factors in sera of patients with pyelonephritis compared to healthy persons.

دراسة مقارنة لعزلات بكتريا Staphylococcus aureus المعزولة من مياه النهر ومن حالات سريرية == A Comparative Study of Staphylosossus Aureus Bacteria Isolated From River Water And From Clinical Cases

Author name: بنين مدلول امانة الزيادي
Supervisor name: علي عبد رحيم الناشي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Higher Diploma
University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: شملت الدراسة جمع 120 عينة اخذت من مصدرين مختلفين, 60 عينة منها جمعت من بيئة مائية هي نهر الديوانية, بينما كانت 60 عينة سريرية جمعت من الاشخاص الراقدين والمراجعين لمستشفى الديوانية ومن الاصابات الجلدية المختلفة التي شملت الدمامل, الحصف, التقشر الجلدي, الخر | The study included assemble 120 samples which had been taken from two different sources, 60 samples collected from An aqueous environment which is Al diwaniya river, where the rest 60 samples have been collected from those people who were admitted to the Al diwaniya hospital and from of different ages from male and female. Where the environment examples collected from different places of riverbed Al diwaniya. The duration of collection samples has lasted for 3 months, started One handrad isolated had been diagnosed that proved its belong to a bacteria Staphylococcus aureus, 50 isolated where taken from environment isolate and the other 50 taken from clinical isolate depending on the diagnostic methods which included tests of farm, microscopic and biochemical, It was also confirm the diagnosis of bacterial using accurate diagnosis that uses device VITEK System tested sensitivity of bacteria isolate of S. aureus, against 12 selected antibiotic, environment and clinical cases has shown resistance and sensitivity of different varied by source isolate, the nature of the antibiotic and the resistance and sensitivity have been determined by measuring diameters of areas inhibition around tablets of antibiotics it used.All the clinical isolates showed a complete resistance for the antibiotic Lincomycin with 100% percentage, where it's resisted Pnicillin G with 92%, Nalidixic acid with 42%, while the highest resistance of environment isolates was 97% for Lincomycin. The clinical isolates showed a sensitively against the two antibiotics which are Cefamandol and Cephalothin, where their percentages reached 98% and 89%, respectively. Where the percentage of environment isolates reached for the same antibiotics 100% and 97% respectively. In general, the clinical isolates were more resisted for the antibiotics than environment isolates in overall rate.differed values of minimum inhibitory concentrationsmic MIC and the minimum bactericidal concentration MBC among each other with variation of clinical isolates from side and differed with environment isolates from another side while the values differed with environment and clinical isolates from third side, where the highest value reached of MIC against Polymyxin B is (20 - 120) and (100 - 240) mg/ml for environment and clinical isolates respectively, while the highest value of MIC that also was against Polymyxin B reached (40 - 140) and (130 - 250) mg/ml for environment and clinical isolates respectively. Where the value of MIC against Cephalothin reached (0.01 - 0.6) and (0.8 - 2.0) mg/ml for environment and clinical isolates respectively. Finally, the highest value of MBC also was against Cephalothin reached (0.08 - 0.9) and (0.9 - 3.0) mg/ml respectively. In general, the bactericidal and inhibitory concentrations of the clinical isolates are higher concentrations from those bactericidal and inhibitory concentrations for environment isolates.I studied the virulence factors that come from strains of S. aureus bacteria environment and clinical which included Capsula, enzymes of Coagulase that are associated and free, , Lipase, Hyaloronidase and B - Lactamase in addition to Haemolysin clinical isolates and Leucocidin and all the environment isolates are the highest percenage in the ability to form and possess virulence factors compared with environment isolates, where the highest percentage to produce virulence factors is belongs to the Haemolysin which reached 94% percentage in the clinical isolates where in the environment isolates reached 64% percentage followed by Leucocidin that reached 84% percentage in the clinical samples and 40% percentage in the In environmental samples.Also the percentage of blood Coagulation enzyme the associated and free is the highest percentage in the clinical isolates which reached percentage 42% and 38% for the associated and free respectively, where the percentage in environment reached 20% and 36% respectively and the virulence factors was the least presence in the environmental and clinical isolates is the portfolio that reached percentage of presence 22% and 12% respectively.

دراسة مقارنة لتاثير بعض المستخلصات النباتية وبعض الاحماض الكاربوكسيلية في البكتريا المسببة لالتهابات الحروق == Comparative Study of The Effect of Some Plants Extract And Carboxylic Acids On Contaminating Bacteria In Burns Infection

Author name: سـعاد خليـل ابراهيـم
Supervisor name: مهدي ضمد القيسي | جنان مجيد العقيدي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:
Abstract: جمعت (500) عينة من المرضى المصابين بالحروق من ثلاث مستشفيات في مدينة بغداد للمدة من 1/6/2007 ولغاية 1/11/2007. زرعت هذه المسحات على الاوساط الزرعية للعزل الاولى وتم تشخيصها بالفحوصات المظهرية والبايوكيميائية. اظهرت (489) عينة ملوثة بالبكتريا وبنسبة | • Five hundreds swabs were collected from patients suffered from burns in three hospitals of Baghdad city, for the period from 1st June 2006 to 1st November 2007. The swabs were implanted in order to be isolated and diagnosed by morphological and biochemical tests. • The results showed that 489 swabs were contaminated with bacteria (97.8%), while 11 swabs were not (2.2%). The identification and prevalence of the bacteria was as follow Pseudomonas aeruginosa was found in 148 swabs (30.27%), Klebsiella pneumoniae in 102 (20.26%), Staphylococcus aureus in 79 (16.16%), Escherichia coli in 70 (14.31%), Proteus mirabilis in 52 (10.63%) and Enterobacter cloacae in 38 (7.77%). • Information card for each patient was assigned included age, sex, time of suffering from burns, social status and address.• The degrees of suffering were as follow : - First degree of burns, 201 cases (40.2%). - Second degree, 187 cases (37.4%). - Third degree, 112 cases (22.4). Sensivity test to antibiotic and was done for all the isolated bacteria, and it was found, that, they were sensitive to Vancomycin, Ciprofloxacin, Amikacin, Tetracyclin and Fusidic acid within the percentage ratio of 0.0, 59.1, 3.7, 58.5 and 11.6%, respectively. Also it was found that the investigated bacteria showed high Vesistance to Penicillin G (87.9%), Erythromycin (93.9%), Trimethoprim (100%), Carbencillin (83.9), Cefotaxime (63.4%), Ampicillin (75.9%), Gentamycin (67.9%), Streptomycin (83.6%), Nalidix acid (89.4%) and Linomycin (83.2%). o Searching for the active compounds in the extracts of investigated plants (Linum usitatissimum, Nigella sativa and Eruca sativa) was conducted. It was found, that, the seeds of the three plants contained Glycosides, Alkalis, Flavonoids, Soapiness and Resins, which are not water soluble, but could be detected by the alcoholic extraction. Cumarin was noticed in the seeds of N. sativa and E. sativa only. o Different concentrations of water and alcohol extracts were prepared in addition to the isolation of oils, soapiness and Flavonids. The effectiveness of the extracts was tested at the concentration of 16, 32, 64, 128, 256 mg/ml for all the plants, against the bacteria.o The Phenol, water and alcohol extracts of L. usitatissimum and E. sativa showed high effect against the bacterial species, while that of N. sativa was less affective.o The Minimum inhibition concentration (MIC), was found to be different according to the type of extract and the species of bacteria.o The effects of the organic acids (Acetic, Citric, and Lactic) were tested at concentrations of 0.5, 1, 1.5, 2, 3 and 4% of each acid. An increase in the diameter of inhibition area, related with the increase in the acid concentrations, was detected. It was found that the acetic acid was more affective against the microorganisms, followed by the Citric acid and then the Lactic acid.o on the base of ratios of inhibition area diameters (in vitro), the results illustrated that E. coli was more affected by Acetic acid when the diameters reached 18.75, 21.75, 30.75, 38.25, 40.00 and 44.75 mm, respectively. The concentration of 1.5% was the effective enough against E. coli as diameters rates for P. mirabilis were 19.25, 18.25, 18.0, 22.56, 27.75 and 31.0 mm, respectively, followed by Ps. aeruginosa, S. aurues and En. cloacae when exposed to the optimum concentrations of Acetic, Citric, Lactic acids. The diameters at concentration of 4% were 23.50 and 23.66 mm. o The water extracts from all the involved plants, in addition to the organic acids showed good levels of effectiveness against the different studied bacterial species, when suffered by burns rats were exposed to them. A clear decrease in bacteria numbers in these rats in comparison with non - treated animals, in the swabs removed from the treated and non - treated rats, in addition to the absence of any histophathalogical changes in livers and skins of the treated animals. o Biochemical tests of serums from animals treated with the water extracts and organic acids at concentration of 16 mg/Kg and 18 mg/Kg for seven days and the microscope investigation, proved that these compounds were not toxic.o The effects of the water extracts and the organic acids were studied too from the point of the immunity point of view. An increase in the Levels of IgG and IgA of the supplementary systems C3 and C4 was noticed in the suffered from burns in general. The levels of IgG and IgA tended to be normal after the treatments with the water extract from the studied plants and with the organic acids.o The L D50 of N. sativa and E. sativa was 18 mg/kg while it was 16 mg/Kg of L. usitatissimum.

دراسة وراثية جزيئية لبكتريا المكورات المعوية البرازية Enterococcus faecalis المعزولة من جذور الاسنان الملتهبة للانسان في بغداد == Molecular Genetics Study of Enterococcus Faecalis Isolated From Root Canal Infection of Human In Bagdad

Author name: سوزان علي كاظم
Supervisor name: عذراء حميد حسون
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:
Abstract: تم التحري عن نسبة وجود بكتريا المكورات المعوية البرازية Enterococcus faecalis في (100) عينة معزولة من اشخاص مصابين بالتهاب قناة جذر السن, اذ تم جمع (70) عينة من الاصابات الابتدائية لقناة الجذر، و(30) عينة من الاصابات الثانوية لقناة الجذر (اعادة العلاج) وم | To detection Enterococcus faecalis in (100) root canal sample were collected from primary and secondary root canal infection patient`s from all the ages (10 - 50) during the period of (August 2013) till (January 2014). Detection depending on cultural & microscobial characteristics of bacterial cell was done to find (45) 0f E. faecalis species & Biochemical tests & Serological diagnosis by Lancefield method done to find (24) isolates of this species & Diagnosis by Vitek2 was done to find (20) isolates of E. faecalis. When the Molecular genetics Diagnosis was done the result showed find (32) isolates belong to E. faecalis. The antibiotic sensitivity test was done by using (14) antibiotics, (5) isolates showed resistant against all antibiotics & the isolates showed multiresistant against for some antibiotics. All the isolates were resistant by (100 %) against (5) antibiotics. To detection of the isolates ability of production of protease enzyme, lipase enzyme, hemolysin enzyme & gelatinase enzyme.The results showed that (24 isolate) (75 %) were protease producer, & (8 isolates) (25 %) were lipase producer, & (16 isolate) (50 %) were hemolysin producer, & (5 isolates) (15.6 %) were gelatin producer. To detection the presence efa A gene of the isolates by used specific primer to this gene, and all isolates

تشخيص بعض النواتج الحيوية لانواع من السيانوبكتريا المعزولة من مياه وترب مدينة تكريت ودراسة فعاليتها على بعض انواع البكتريا المرضية والحيوانات المختبرية == Identification of Some Bioproducts From Some Species of Cyanobacteria Isolated From Water And Soil From Tikrit City And Study Their Biological Effects On Some Pathogenic Bacteria And Laboratory Animals

Author name: ايمن عوني سليم جاسم
Supervisor name: حميد سلمان خميس المهداوي | احسان محمود عبد الله
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Tikrit University - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص (19) نوع من السيانوبكتريا من عينات مياه وتربة طينية ومن الصخور من (5) خمسة مواقع في مدينة تكريت ضمن محافظة صلاح الدين، وقد تم اختيار ثلاثة انواع من هذه السيانوبكتريا وهي : Anabaena oryzae, Nostoc linckia, Anabaena laxa اعتم | The current study included Isolation and Identification of (19) species of cyanobacteria from water, clay soil and rocks samples, collected from (5) sites in Tikrit, salah Al - den province. Three species were chosen each species represent the environment which collected from it. These species were Anabaena oryzae, Nostoc linckia, Anabaena laxa. The effects of some environment factors on growth and biological products quantity has been studied in the three species above. The effects of the temperature showed that 26°C was the best for growth and biological products quantity than 16°C and 36°C. While the pH effects showed that pH 7.6 was the best for growth and biological products quantity than pH 6.6, 8.6. Three light intensities were used 1250, 2500, 5000 lux were used. The results showed that the light intensity of 2500 lux was the best for daily growth and quantity of extracted biological products. While the study of the effects of addition of macronutrient and micronutrient to Asm - 1 media which used for culturing of the three species of cyanobacteria by replacement of NaCl by NaNO3 in the medium, showed that there is an increase in daily growth and quantity of extracts of biological extracts, also the addition of FeSO4.7H2O and NaNO3 together showed further increase of daily growth and biological extracts quantity. The biological products has been identificated by HPLC - MS, IR and NMR. The results showed the presence of the neurotoxin Anatoxin - a and the hepatic toxin Microcystin in its various forms MCYST - LAba, MCYST - YM(O), [D - Asp3, Dha7]MCYST - LR and MCYST - YA in the biological products of Anabaena oryzae. The results also showed the presence of the hepatic toxin MCYST - YM(O) and [D - Asp3, Dha7]MCYST - LR in the extracts of the two spices of cyanobacteria Nostoc linckia and Anabaena laxa.The results also showed that the Muller Hinton Agar media is better than the Blood Agar media in testing the activity of these extracts in inhibition of the pathogenic bacteria, the method of holes is better than saturated discs, and the solvent DMSO (Di - Methyl Sulpho Oxide) is the better in testing of biological activities, while the filtrate of cyanobacteria did not shown any activity for inhibition of the pathogenic bacteria which include : Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus Vulgaris, Staphylococcus aureus. The results showed the high capability of inhibition in concentrations of 1000, 2000, 3000 µg/ml of the extracts of the cyanobacteria Anabaena oryzae, Nostoc linckia and Anabaena laxa on the above pathogenic bacteria and some times more efficient than antibiotics used for comparison, and the concentration 3000 µg/ml was the most effective.The effects of biological extracts of the three species in concentrations of (1000, 2000, 3000) µg/kg of body weight on tested albino mice, using injection and feeding methods, was studied. The results showed poisoning nervous symptom for the first few hours, while the results showed hepatic poisoning symptom on the dissecting animals. The anatomic symptoms included changes the colour of the liver to darkening and appearance of necrosis, in addition to becoming lobbed and swollen some times with bleeding.While the poisoning effects on dissecting tissues of livers of the animals showed a simple swollen and some times assured in all of the liver accompanying with increase in growth and cell division, as well as increases in lymphatic cells inside the tissue and in general in liver tissue. The lymphocyte seemed bigger than for normal cell with multi - nuclei in white blood and increase in cell size and destruction of liver tissue. Nostoc linckia was the greater urging for cell division consequently probably more induce of cancer in the liver of the animals, while the biological product of Anabaena laxa showed more poisonous for liver cell than the biological products of Anabaena oryzae, the least effective in cytotoxic and urging cancer cell in liver of the animals.

العلاقة بين انتاج الانزيم المحلل للكولاجين وتكوين الغشاء الحياتي بوساطة بكتريا Pseudomonas aeruginosae == The Relationship Between Collagenase Production And Biofilm Formation By Pseudomonas Aeuroginosa

Author name: امال عزيز كريم السعدي
Supervisor name: شذى سلمان الطحان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: A total of 359 samples divided as 228 clinical and 131 non clinical specimens were collected during 2012 from four hospitals in Baghdad city including : Al - Kadhymia Teaching hospital, Baghdad Teaching hospital, The Burn Specialist Hospital and Al - Imam Ali hospital, for isolation of P.aeruginosa to study the correlation between collagenase production and biofilm formation. Eighty two Pseudomonas isolates were screened for biofilm formation, 28 isolates were classified as strong biofilm formers, 25 as moderate and 27 as weak biofilm former. The 28 isolates were identifid by VITEk - 2 Compact system which confirmed that the isolates were P.aeruginosa. Collagenase production assay was used to screen 28 isolates that were strong biofilm formers inorder to detect the ability of these isolates to produce collagenase, the substrate of collagenase (collagen) was purified localy from bovin tendon and the results showed that just 8 isolates could grow in mineral salt media with collagen after 4 days of incubation. The factors affecting biofilm formation and collagenase production were studied to determine the optimual conditions for their production, those factors included : 1 - Nitrogen sources represented higher influence on collagenase production specialy (yeast extract) in media containing collagen than other media without collagen as a substrate. The specific activity differed between the 8 isolates, biofilm formation also became more pronounced with (yeast extract), while NH4Cl and NaNO3 depressed biofilm formation at the same conditions. The statistical analysis between the two parameters (biofilm and collagenase) according to different nitrogen sources demonstrated highly significance at p?0.01 with yeast extract and casein. 2 - pH, results showed that the best pH for production was 7 for both collagenase and biofilm.The statistical analysis for determination the relationshipe between the two parameters showed highly significance at p ?0.01 for different pH. 3 - The maximum production of the two parameters was at 35?C temperature which gave highly significance at p?0.01 with defferent temperature. 4 - Long incubation periods revealed increasing in collagenase production and biofilm formation which represented highly significance detween them when incubation periods were prolonged at p?0.01. Results of this study showed that collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction. The REP - PCR analysis using BOX - primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP - PCR in 9 different genotypes, named cluster 1 to 3 which included C1, C2, C3 with relatedness : 8 (80%), 8 (86%), 3 (80%) respectively. A19 and A20 both of them were not included in any cluster, they have 78% similarity.The REP - PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13), showed similarity reached 86

تاثير اشعة الفا على التصاقية بعض انواع البكتريا == Study The Effect of The Alpha Ray On The Adhesion Some Type of Bacteria

Author name: فردوس غضبان كاظم
Supervisor name: زياد متعب الخزاعي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Higher Diploma
University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية جمع 37 مسحة من المرضى المصابين باخماج العيون في مدينة الديوانية ومن كلا الجنسين حيث تضمنت الدراسة سيادة نوعين من المسببات البكتيرية لاصابات العيون ودراسة قابلية التصاق هذه البكتريا على العدسات اللاصقة وهي المكورات العنقوديـــــــــــ | The current study included (37) samples were collected from sicks of Eyes infection in Al - Diwaniya city from two kinds that the study included two type dominant of Bacteria causes for Eyes infection and study this Bacteria adhesion ability on the Lenses, that the Staphylococcus aureus and Pseudomonas aeruginosa as well as the impact of exposure to (Radiology Alpha and Gamma rays) on adhesion ability, As well as the search included the application of a mathematical model to adhesion formula. The current study results are showed the Staphylococcus aureus for percent (45.94)% and then the Pseudomonas aeruginosa for percent (32.43)%. The results were obtained being clear impact of irradiation on Bacteria adhesion ability to Lenses. The results were obtained the higher level adhesion reaching its, the Staphylococcus aureus in the natural case to (cfu/mm2 10 - 6×292) that in three hours whereas the Alpha ray exposure more than adhesion level that range six hours to (cfu/mm2 10 - 6×302) and when the Gamma ray exposure the higher adhesion level reaching that range (cfu/mm2 10 - 6×233) during exposure period that reach to four and half hour. About the Pseudomonas aeruginosa the adhesion higher level in the natural case to (cfu/mm2 10 - 6×204) during two hours and half hour whereas when exposure its, to Alpha ray that reaching to higher level adhesion range (cfu/mm2 10 - 6×298) during six hours as well as when exposure to Gamma ray the higher level adhesion reaching range (cfu/mm2 10 - 6×250) during four and half hour. Application the mathematical adhesion sample, the result obtained that the disengagement coefficient, the valuable impact its, so that the valuable increased as well as temporal to be late in approach stage of zero who mathematically certain the lenses importance that the important dangerous effective for Eye infection distribution. As well as the results were certain the Alpha and Gamma rays were irradiation, that have impact of where decreasing the adhesion bacteria numbers for lenses, that decreasing from Eyes infection

دراسة مظهرية ووراثية عن بكتريا Enterococcus faecalis المعزولة من مصادر سريرية مختلفة في مدينة الديوانية == Phenotypic And Genotypic Study On Enterococcus Faecalis Isolated From Different Clinical Sources In Al - Diwaniyah City

Author name: حيدر سعود مايح الكرعاوي
Supervisor name: ازهار نوري حسين الموسوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: جمعت 280 عينة من حالات سريرية مختلفة من مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية للفترة من 20/11/2012 لغاية 12/4/2013، لغرض عزل وتشخيص المكورات المعوية البرازية Enterococcus faecalis ودراستها مظهريا ووراثيا. اذ عزلت وشخصت 20 ع | Two hundred eighty samples were collected from different clinical cases of Diwaniyah Educational Hospital and Maternity and Children Hospital in the city of Diwaniyah, during the period from 20/11/2012 till 12/04/2013. 20 isolates of Enterococcus faecalis were isolated and identified.This isolates distributed on : 10 isolates from urinary tract infections and 10 isolates from cases of diarrhea while not isolated from wound and burn infections. The results of morphological cultures and microscopic study, biochemical tests, In addition to the use of diagnostic system Histrep and then the final diagnosis by a Vitek 2. Molecular diagnosis showed that all isolates contained 16S rRNA gene. Susceptibility test was done for all bacterial isolates against (9) antibiotics. The results showed that all isolates were complete resistance (100%) against Erythromycin, Tetracycline, Rifampin and Ciprofloxacin. While its resistance for other antibiotics was varied. The resistance isolates for Chloramphenicol, Nitrofurantoin, Penicillin, Gentamycin and Vancomycin were 50 %, 85%, 60 %, 70 % and 35 %, respectively. The results showed that the total resistance for E.faecalis isolates was higher than the sensitivity (more than 70% of these antibiotics). Polymerase chain reaction was used for detecting some genes for antibiotics resistance. The results showed that 7 bacterial isolates had van gene which were resistance to Vancomycin. 6 isolates of these bacteria contained vanB gene while one isolate contained vanA gene. Also the results showed that the bacterial isolates had antibiotics resistance genes as follow : aac (6) - aph (2) gene responsible for resistance to Gentamycin, cat gene responsible for resistance to Chloramphenicol, erm (A)gene responsible for resistance to Erythromycin, and tet (M) gene responsible for resistance to Tetracycline were 70%, 50%, 45%, 100% respectively. Some virulence factors had been detected for bacterial isolates including the ability of isolates of haemolysin production, protease production, gelatinase production and the ability to capsule formation. The results showed that 5 isolates (25 %) its ability of producing was type beta, and 15 isolates(75%) its ability of producing protease, 8 (40%) its ability of producing the gelatinase , and 5 isolates (25%) had the ability on capsule formation. The investigation for these virulence factors in genotypic method, the results showed that bacterial isolates had cylA gene responsible for the production of haemolysin, asal gene responsible for the production of aggregation substance, esp gene responsible for the production of Surface proteins for Enterococcus faecalis and hyl gene responsible for the production of hyaluronidase the percentage 25%, 90%, 55% and 5% respectively.

دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط == Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

Author name: ابرار علي حسين
Supervisor name: هيام عبد الرضا كريم العواد | ياسمين خضير الغانمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Karbala
First pages:
Abstract: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples

عزل وتشخيص بكتريا Streptomyces المنتجة للمضادات الميكروبية من ترب بعض اقضية محافظة صلاح الدين == Isolation And Identification of Antimicrobial Producing “Streptomyces” From Soils of Some Districts In Salahaddin Province

Author name: هيام عزيز عباس البدري
Supervisor name: عبد الكريم فتاح عمر
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: Tikrit University - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة عزل وتشخيص انواع تابعة للجنس Streptomyces، اذ جمعت 50 عينة من ترب اقضية تابعة لمحافظة صلاح الدين. شخصت?11 نوع من بكتريا Streptomyces باستخدام الاختبارات الشكلية والمزرعية والكيموحيوية حيث ظهر كل من الانواع (Streptomyces antibioticus, Strep | The Study involved ; isolation and characterization of species related to Streptomyces. Fifty samples of soil has been taken from different districts of Salah aldden province. The isolates were identified by the morphological, biochemical tests and classified to nine species, The following species were found : (Streptomyces antibioticus, Streptomyces coelicolor , Streptomyces distalicus and Streptomyces fluvissimus ) in Tikrit district, ( Streptomyces cyaneus ) in Ballad and beiji districs, ( Streptomyces exofolicus) in Ballad and Aldor districts (Streptomyces lavendulea ) in Samarra and Aldor districts , (Streptomyces purpureus ) in Samarra and Beiji districts and (Streptomyces violaceus ) in Samarra and Tikrit districts. The sensitivity of these bacterial isolates were tested toward the following : (Azithromycin, Cefotaxime, Cephatexin, Doxycycline, Erythromycin , Gentamicin, Neomycin, Penicillin, Tobramycin and Vancomycin).All species showed high resistance to cefotaxime. But all species were sensitive to neomycin and doxycycline. The isolated species of Streptomyces were tested for their ability of antibiotic production. It was found that (Klebsiella) was sensitive to all the species except species Streptomyces coelicolor. Streptomyces coelicolor showed an effect against Proteus. While Staphylococcus aureus was sensitive to the following species (Streptomyces antibioticus, Streptomyces cyaneus and Streptomyces purpureus). The Scanning Electron Microscopy was used to study the surface of spores. The species of (Streptomyces antibioticus) was found to has warty surface while spores of ( Streptomyces coelicolor) has smooth surface.

الاصابة ببكتريا Helicobacter pylori وعلاقتها بمرض تصلب الشرايين القلبية == Correlation Between Helicobacter Pylori Infection And Atherosclerotic Heart Disease

Author name: رواء سعدي سلمان
Supervisor name: مي خليل اسماعيل
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: مرض قصور الشرايين التاجية هو النتيجة النهائية لتراكم اللويحات العصيدية ضمن جدران الشرايين التاجية مسببة نقص الاوكسجين وبالتالي حدوث مرض القلب الاقفاري. ويعتبر من الامراض الشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم. اضافة الى عوامل الخطورة التقلي | Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in shortage of oxygen supply and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of worldwide morbidity and mortality. In addition to traditional risk factors for cardiovascular disease, nowadays, accumulating evidence indicates that a variety of infectious agents may contribute to pathogenesis of CAD. The present study evaluated the anti - Helicobacter pylori IgG and IgA and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) and vacuolating associated cytotoxin (Vac A) as a risk factors for CAD.Eighty patients were divided into 2 groups : first group was 70 patients with CAD, the other group contained 10 patients with another coronary artery disease; they were admitted to Ibn Al - Bitar Specialist Center for Cardiac Surgery in Baghdad between October 2013 and January 2014. Ten individuals used as a healthy control group. All blood samples were tested biochemically such as glucose test, urea test, creatinine test and lipid profile test(cholesterol, triglyceride, LDL and HDL) and all of them showed normal results. The present study revealed that males were affected more than females (3 : 1); with no relation between their ages and bacterial infection.Serum IgG and IgA was Estimated by indirect immunofluorescent (IIF) whereas Cag A, Vac A and high sensitive C - reactive protein (hs - CRP) measured by enzyme linked immunosorbent assay (ELISA) and compared to control group results.The incidence of the anti - H.pylori IgG was highly significantly prevalent in CAD patients 78.57% (55/70), than in control group 0% (0/10), also a high significant difference in another CAD patient group 100%(10/10) as compared to control group 0% (0/10) (P? 0.01).Similarly anti - H. pylori IgA in CAD patients and another CAD patient groups showed highly significant increase when compared to control group 37.14% (26/70), 30% (3/10) and 0% (0/10) respectively (P? 0.01).The incidence of the CagA - positivity was significantly prevalent in mean value (2.74±0.19) in patients with CAD and another CAD patient group(2.72±0.31) than in control group (1.64± 0.16) (P? 0.05). Same result was observed with Vac A antigen; mean value of patient group showed significant increase (1.299 ± 0.04) when compared to controls group (1.41±0.13) (P? 0.05). Also significant increase found between another CAD patient group(1.79±0.17) and control group (1.41±0.13) (P? 0.05). Furthermore; the present study revealed significant differences of concentration levels between hs - CRP in CAD patient group (4.95 ± 0.38 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml), as well as a significant differences found between another CAD patient group (3.96 ± 0.96 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml) (P? 0.05).

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

Author name: محمد خضير عباس النعيمي
Supervisor name: عباس عبود فرحان الدليمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Diyala
First pages:
Abstract: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

تحديد الصفات المظهرية والوراثية لانواع بكتريا المكورات المعوية المعزولة من المرضى في محافظة النجف الاشرف == Phenotypic And Genotypic Detection of Enterococcus Sp. Isolated From Patients In Al - Najaf Al - Ashraf Governorate

Author name: زهراء حميد عودة القريشي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kufa - College Of Science - Department Of Biology
Language: English
University location: Najaf
First pages:
Abstract: The study aimed to isolate and identify the Enterococcus spp. from different clinical specimens and study the virulence factors of predominant species, as well as detected the virulence factors encoding genes such as efaA (endocarditis - associated antigen), esp (enterococcal surface protein), eep (stimulating of pheromones expression) and enlA (enterolysin A) genes by PCR techniques.There were three hundred clinical specimens collected from patients suffering from different clinical infections during the period from September 2013 to January 2014 in AL - Sadder Medical City and AL - Hakem General Hospital. The identification of the Enterococcus spp. isolates were depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed with the automated VITEK - 2 compact system using Gram positive - Identification (GP - ID) cards.According to the results obtained by the VITEK tests forty two clinical isolates of Enterococcus were detected, which distributed into : (22) isolates from urine, (8) vaginal swabs, (6) seminal fluid, (4) throat swabs and two isolates from wound swabs with no isolates from cerebral spinal fluid, stool and blood specimens.This study revealed that the E. faecalis is more distributed with 25(59.52%), followed by E. faecium with 10 (23.80%), E.avium with 5 (11.90 %), E. durans and E. raffinosus with 1(2.39%) for each.The study investigated the virulence factors of E. faecalis, E.faecium and E.avium, which play a major role in enterococcus pathogenicity. E. faecalis and E.faecium had the ability to producecapsule, gelatinase, biofilm, adhesion, protease, bacteriocin, haemolysin and cytolysin except ? - lactamase produced only by E. faecalis while E.avium produced all these virulence factors except gelatinase, bacteriocin and cytolysin.The results revealed variation in the resistance of bacteria to antibiotics, E.faecalis express absolute resistance (100%) to Erythromycin, high resistance against Gentamycin, Tetracyclin and Vancomycin but high susceptibility to Ciproflaxacin and Penicillin and moderate susceptibility to Chloramphenicol. E.faecium exhibited absolute resistance (100%) to Erythromycin, Gentamycin and Tetracyclin, high resistance against Chloramphenicol, Penicillin and Vancomycin but high susceptibility to Ciproflaxacin while E.avium exhibited susceptibility (100%) to all these antibiotics except Tetracyclin showed absolute resistance.Then detected the virulence factors encoding genes : efaA, esp, eep and enlA genes by using PCR techniques and Electrophoresis Systems.Finally, The genotypic method. Regard to genotypic study the outcome showed that 21(84%) isolates of E.faecalis, 4(40%) of E.faecium and 5(100%) of E.avium were carrying efaA gene and 17(68%) of E.faecalis, 5(50%) of E.faecium, 5(100%) of E.avium were carrying esp gene while eep gene was carrying only by E.faecalis and E.faecium ; 12(48%) and 3(30%) respectively. Also the results revealed that only 1(4%) isolates of E.faecalis have enlA gene.

العلاقة بين مرض السكري المناعي الذاتي الكامن النمط الثاني للبالغين مع الخمج بالملوية البوابية == Association Between Latent Autoimmune Diabetes Of Adults In Type Ii Dm With Helicobacter Pylori Infection

Author name: سيف علي مكلف
Supervisor name: عروبة خالد عباس | مدين علي حسين
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:
Abstract: Latent Autoimmune Diabetes in Adults (LADA) is a form of diabetes which is less recognized and underdiagnosed type of diabetes which appears to have characteristics of both type 1 (autoimmune in nature) and type 2 diabetes (adult age at onset).Helicobacter pylori (H. pylori) infection has a worldwide distribution. The bacteria colonize more than half of the world population and consider the main cause of gastritis, peptic ulceration, gastric adenocarcinoma and gastric lymphoma. The host immune response is unable to clear the infection and may actually contribute to the associated pathogenesis, also the bacterial virulent factors had certain role in the pathogenicity of infection as it was stated by limited studies that the Cytotoxin associated gene A (CagA) protein and Vacuolating cytotoxin gene A (VacA) protein positive strains are possibly associated with severe infections.The present study was designed to find out the percent of LADA patients among group of type II Iraqi diabetic patients and the relation between LADA patients and H. pylori infection.The study was carried out on 350 type II patients attending the National Diabetes Center/ Al - Mustansirrhea University/ Baghdad Al - Yarmouk during the period May to October 2014 were recruited for this study. Diagnostic markers for LADA, namely, C - peptide levels by Radioimmunoassay and GAD autoantibodies by ELISA test were determined and other Various parameters such as, HbA1c levels, Anti - H.pylori IgG, Anti - CagA protein and Anti - VacA protein by ELISA test were evaluated and correlated with type 2 diabetes and 40 control, matched by age and gender were also included.57 patients of 350 of type II have low C - peptide level, from which, 40 patients have Anti - GAD65 positive and 17 patients have Anti - GAD65 negative.The percentage of LADA patients among type II diabetes was 11.4%.The mean serum levels of C - peptide of LADA patients, controls and type II diabetes (0.30 vs. 1.94 and 3.15ng/ml) were significantly lower in LADA patients as compared to controls and type II patients (p?0.05).Also the mean blood concentrations of HbA1c of LADA patients, type II and controls (8.5 vs. 7.5 and 5.2 %) were significantly higher in LADA patients as compared to type II patients and controls (p?0.05).The Higher frequency of anti - H. pylori IgG was in LADA patients when compared with patient's controls and healthy controls (59.6%, 21.3% and 19.1%) and the p - value (?0.05).In conclusion, Cytotoxic associated gene A protein (Cag A) protein is more frequency with H. pylori positive patients 36/47 (76.6%) indicate an important role of this virulence factor in the pathogenesis of H. pylori, so they could be considered as a good biomarker for discern the risk of developing serious gastroduodenal disease in the host, than Vacuolating cytotoxin A Protein (Vac A) which is less frequency than Cag A in H. pylori positive patients 16/47 (34%).

التحري الجزيئي للجزر الوراثية المسؤولة عن المقاومة للمضادات الحياتية في العزلة baumannii A92 Acinetobacter == Molecular Detection Of Genomic Islands Responsible For Antimicrobial Resistance In Acinetobacter Baumannii Strain A92

Author name: سهاد سعد محمود العجيلي
Supervisor name: اليس كريكور ملكونيان | علي حسين ادحيه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

مقارنة الطرائق التشخيصية الوسط اللوني Chromogenic mediumوالانتشار بالاقراص DisK Diffusion المتسلسل PCR للكشف عن المكورات العنقودية الذهبية المقاومة للمثيسيلين في الردهات الجراحية في مدينة الناصرية == Comparison Of Chromogenic Medium, Disk Diffusion Test And Pcr Methods Forthe Detection Of Methicillin Resistant Staphylococcus Aureus (MRSA) In The Surgical Wards Of Al - Nasiriyah City

Author name: جواد رشید هویدي الزیدي
Supervisor name: امین عبد الجبار السلمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: English
University location: Basrah
First pages:

دراسة فسلجية لبعض انواع من جنس Bacillus المقاومة للمعادن الثقيلة وتحسين قابليتها الاصحاحية == Physiological Study Of Some Bacillus Spp. Resistant To Heavy Metals And Improving Their Ability Of Bioremediation

Author name: ایمان عبعوب مخیفي
Supervisor name: امین عبد الجبار السلمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:

عزل وتشخيص جرثومة Helicobacter pylori من مياه الصرف الصحي في محافظة البصرة باستعمال الطريقة الزرعية وتقنية ال CR ( 16S rRNA ) == Isolation And Identification Of Helicobacter Pylori From Sewage In Basrah Province By Culture Method And PCR (16S Rrna)

Author name: الاء يعقوب الربيعي
Supervisor name: امين عبد الجبار السلمي | اسعد محمد رضا الطائي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:

القرابة الوراثية لجراثيم Acinetobacter baumannii المعزولة من مدينة الطب في بغداد == Genotyping Relatedness Of Acinetobacter Baumannii Isolated From Medical City - Baghdad

Author name: نظيمة حمود حسين السليم
Supervisor name: حارث جبار فهد المذخوري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

دراسة تشخيصية لبكتريا Helicobacter pylori المسببة لمرض التقرحات المعدية في محافظة صلاح الدين == Diagnostic Study for Helicobacter pylori Bacteria That Cause Gastric Ulcers in Salah Aldeen Governorate

Author name: مصعب علي جواد المهداوي
Supervisor name: امين سليمان بدوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: Tikrit University - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:

تاثير بعض مبيدات الادغال والحشرات في نمو ونشاط بكتريا العقد الجذرية في نبات الباقلاء Vicia faba == EFFECT OF SOME HERBICIDES AND INSECTICIDES ON GROWTH AND ACTIVITY OF ROOT NODULES BACTERIA ON Vicia faba PLANT

Author name: انمار سعدي عبود العكيدي
Supervisor name: نضال نعمة حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:

عزل وتشخيص الجراثيم من عينات سريرية وبيئية والكشف عن بعض العناصر الوراثية المسؤولة عن المقاومة للمضادات الحيوية والعناصر الثقيلة في محافظة البصرة == Isolationand Identification of Bacteria from clinical and Environmental Samples and Detection of Some Genetic elements Responsible for Antibiotic and Heavy Metals Resistance in Basrah Government

Author name: اسماعيل جمعة عباس
Supervisor name: ميثم ايوب الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

التلوث بالبكتريا الهوائية المرضية المعزولة من الجروح ما بعد العمليات والحروق والتعرف على بعض مصادرها وخاصة الكلبسيلا الرئوية في محافظة ذي قار == The Contamination by Aerobic Pathogenic Bacteria Isolated from Postoperative Wounds and Burns and Study some of Their Sources Especially Klebsiella pneumoniae in Thi-Qar province

Author name: خوام ريسان حسين الحسيني
Supervisor name: Myad F. Nakkash | Ilham s. Abdul Karim
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: English
University location: Baghdad
First pages:

انتشار صفة المقاومة لمضادات البنسلينات والسيفالوسبورينات لبعض انواع البكتريا السالبة لصبغة غرام المنتجة لانزيمات البيتالاكتاميز عند النساء اللواتي يعانين من التهاب المهبل == Dissemination of Penicillins and Cephalosporins Resistance among Some Gram-Negative Bacteria, Producers of -Lactamases, in Women of vaginal Inflammation

Author name: وليد شمسي حسين المشهداني
Supervisor name: حسين حسن عمر خانقاه | فاروق خالد حسن العكيدي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

دراسة بعض الجوانب البكتيرية والمناعية لخمج المجري البولي المزمن == Study of Some Bacteriological and Immunological Parameters in Chronic Urinary Tract Infection

Author name: محمود ابراهيم اسماعيل
Supervisor name: خليل مصطفى خماس | علي حسين ادحيه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

التاثيــر البايولوجي لســموم بكتريا ذات الرئــة على الفئران البيضاء (داخل الجســم وخارجـــه ) == Biological Effects Of Klebsiella Pneumoniae Toxin In Albino Mice (In Vivo & In Vitro)

Author name: محمد سلمان محمد صريح
Supervisor name: عصام فاضـل الجميلــي | ناهــي يوســف ياســين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: English
University location: Baghdad
First pages:

دراسة بكتيريولوجية وكيموحيوية ونسيجية لتاثير حامض اللاجيك المستخلص من قشور الرمان على بعض عزلات بكتريا الكوليرا Vibrio cholerae وانزيم البروتييز == Bacteriological, Biochemical, and Histological study for the effect of Ellagic acid extracted from Punica granatum peals on some Vibrio cholerae isolates and protease

Author name: لقاء حميد مهدي الموسوي
Supervisor name: مهدي ضمد محيسن | سوسن حسن عثمان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

دراسة بكتريولوجية وكيموحيوية لبعض عوامل الضراوة المنتجة من بكتريا Vibrio cholerae المعزولة محليا من المرضى المصابين بالهيضة

Author name: كفاح احمد جاسم الكرخي
Supervisor name: غازي منعم عزيز | حسين حسن خانقاه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

دراسة بكتريولوجية، مرضية ونسيجية لحالات التهابات الزائدة الدودية == Bacteriological and Pathohistological Study on Appendicitis

Author name: غادة حسن برهان
Supervisor name: رعد خليل الحسيني | علاء غني حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

دراسة جزيئية ووراثية لعوامل الضراوة لبكتريا Acinetobacter المعزولة من خمجات مرضية مختلفة

Author name: عباس شاكر جواد المحنة
Supervisor name: سوسن ساجد الجبوري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: Arabic
University location: Baghdad
First pages:

التحري عن وجود جرثومة Staphylococcus Saprophyticus في حالات اصابات الجهاز البولي في الانسان وبعض الحيوانات == Investigation about STAPHYLOCOCCUS SAPROPHYTICCUS in urinary tract infection in human and some animals

Author name: عبد الكريم سلمان صكبان اليساري
Supervisor name: حسن علي عبد الرضا | محمد صبري عبد الرزاق
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
First pages:

عزل وتشخيص جرثومة Salmonella Typhimurium والكشف عن الجين المشفرلتخليق النوع الهدبي الاول باستعمال تقنية تفاعل سلسلة البوليمريز == Isolation and Identification of Salmonella typhimurium and detection of gene encoded Type - 1 - fimbriae by using Polymerase Chain Reaction

Author name: حيدر علي محمد الكرعاوي
Supervisor name: عدنان موسى الروضان | عدنان حمد الحمداني
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: Arabic
University location: Basrah
First pages:

عزل وتشخيص جرثومة Burkholderia pseudomallei من حليب الابقار والمعز والبيئة المحيطة بها في محافظة البصرة == Isolation and Identification of Burkholderia pseudomallei from Cow and Goat’s Milk and Their Surrounding Environment in Basrah Province

Author name: هناء خليل ابراهيم
Supervisor name: عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: Arabic
University location: Basrah
First pages:

دراسة جرثومية مصلية عن اهم مسببات الاجهاض في النساء مع التركيز على داء البروسيللوسيز == Bacteriological and Serological Study of Important Causes of Abortion in Women with Concentrated on Brucellosis

Author name: لمى حكمت كريم البياتي
Supervisor name: غازي موسى الخطيب
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
First pages:

Extraction And Purification Of Salmonella Spp. Enterotoxin Isolated From Bovine In Basrah Province

Author name: مؤيد حنون صيهود
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: English
University location: Basrah
First pages:

عزل وتشخيص بعض جراثيم الفلورا الطبيعية من كرش وامعاء بعض المجترات والكشف عن الفعالية الضد ميكروبية لجرثومة الايشيريكيا القولونية == Isolation and Identification of Some Normal Flora From The Rumen and Intestine of Some Ruminants and Detection of The Antimicrobial Activity of Escherichia coli

Author name: عبد العزيز صالح الشمري
Supervisor name: داوود سلمان علي | عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: Arabic
University location: Basrah
First pages:

الالفة الجينية لبكتريا ايشيريشيا القولون الممرضة للسبيل البولي المتعددة المقاومة للمضادات الحياتية المعزولة من التهاب المجاري البولية في النجف == Genetic Affinities Of Multiple Drug Resistant Uropathogenic Escherichia Coli Isolated From Patients With Urinary Tract Infection In Najaf

Author name: سامر عبد الصاحب محمد حسين الهلالي
Supervisor name: علي محسن نعمة المحنة
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Najaf
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التحري عن البراعم الحرشفية والجراثيم الاخرى في خمج الجهاز التناسلي لدى النساء == Detection Of Chlamydia And Other Bacteria In Genital Tract Infection Of Females

Author name: اسماء زكي شيتاوي الغريري
Supervisor name: زين العابدين عبد العزيز عبد الله
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al Mosul - Faculty Of Medicine
Language: English
University location: Mosul
First pages:

قياس كمية المستضد الخاص بالبروستات الكلي والحر مع معايير اخرى لدى المرضى المصابين بتضخم البروستات الحميد والسرطان == Estimation Of The Total And Free PSA Together With Other Parameters In The Diagnosis Of Benign Hypertrophy And Malignancy Of The Prostate

Author name: فراس محمد داود الطائي
Supervisor name: زين العابدين عبد العزيز عبد الله
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al Mosul - Faculty Of Medicine
Language: English
University location: Mosul
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التحري عن الاصابة باللولبيات البوابية لدى مرضى عسر الهضم بطرق مصلية جرثومية مختلفة == Detection Of Helicobacter Pylori Infection in Dyspeptic Patients By Different Serobacteriological Methods

Author name: ايمان محمد مجيد
Supervisor name: زين العابدين عبد العزيز عبد الله
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Al Mosul - Faculty Of Medicine
Language: English
University location: Mosul
First pages:

دراسة مقارنة لفعالية مضادات حياتية مختارة تجاه بعض انواع المكورات العنقودية ذات المقاومة المتعددة == Comparative Study of The Activity of Selected Antibiotics Against Some Multi-Drug Resistant Staphylococcal Species

Author name: نورتان عبد الصاحب جمعة الغريب
Supervisor name: ميثم ايوب الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - Faculty Of Education
Language: Arabic
University location: Basrah
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الدور الوقائي المتعدد السكريد الشحمي البكتريا seudomonas aeruginost ضد خمج قرنية العين

Author name: جنان محمد حسن العزاوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

تاثير التلوث البكتيري لنهر ديالى في بيئة نهر دجلة

Author name: ايثار كامل عباس الميالي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

دراسة تاثير بعض العوامل البيئية والمواد الكيميائية الحافظة في نمو البكتريا Escherichia الممرضة COLI

Author name: علياء رزوقي حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

تداخل تاثيرات المواد الحافظة في حماية بكتريا التسمم الغذائي Staphylococcus aureus ضد الضرر الحراري

Author name: فاتن فرج
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master

الاحوال (الظروف) المناسبة للحفاظ على الفاسشيولا جاجينكا في الاوساط الخالية من البكتريا

Author name: نهلة حنا العفاص
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

خواص انزيم الفوسفتيز القاعدي للمكورات العنقودية الذهبية المسببة لالتهاب الضرع في الابقار العراقية

Author name: احلام عبد الرزاق محمد
Supervisor name: فاروق خالد حسن
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
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