دراسة تجريبية وجزيئية للبكتريا المصاحبة لحالات الحروق في محافظة ميسان == Experimental and Molecular Study of the Bacteria associated with burns in Maysan Governorate
Author name:
حسين علي دنانه البهادلي
Supervisor name:
رحمن لعيبي جلاب | حيدر خميس شنان
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Dhi Qar
First pages:
24T3039 - p.pdf
Abstract:
Burn injuries are considered as health problems that may sometimes lead to rapid death. Bacteria are considered as the most important causes of microbial infection associated with burns. In present study, 75 clinical samples were collection from patients with burns for the period from October 2015 to June 2016 from the lobby burns in Sadr Teaching Hospital in the Maysan Governorate, these burn swabs revealed 56 bacterial isolates, 41 (73.21%) were Gram - negative bacterial, distributed of (35.71%) Pseudomonas aeruginosa, (28.57%) Klebsella Pneuomonia, (3.57%) Escherichia coli ,Enterobacter (5.35%). On the other hand 6(10.71%)were Gram - positive bacterial. out of 75 burned swabs, 84% revealed a single pathogens and 16% shown a mixed bacteria. On the other hand, Antimicrobial susceptibility for the bacterial burn isolates revealed that P. aeruginosa was resistant to routine tested antibiotics, like Amoxicillin, Gentamycin and Tobramycin in 100%, While was resistance to Ciprofloxacin 85%, while most P. aeruginosa was sensitive to Ceftizmiden in 50%. Results also showed that Staph. aureus was resistant to Gentamycin, Tobramycin, and Levofloxacin in 50% respectively.Depending on the rate of injury burns microbial and resistance to antibiotics used, p.aeruginosa and Staph.aureus isolated from the burnswere chosen and study their role in the events of infection, experimental burns, and for this purpose has been the use Albino mouse male as a model for a pilot study for pathogenesis and progress associated with the infection of the disease bacterial burns. (33) male mice were divided into tow groups, the first group(24) mice were burned (deep dermal second degree) and infection by 0.2X108 cfu kg of elected isolates, the route of infection was subcutaneously and scratching within burned area. In the second group six were infected without burn, which was considered as positive control, while in the thirdgroup three mice were burned and injected by 0.2ml of normal saline and consider as negative control .the first group was subdivided into two subgroups (12) mices for each burn dominant pathogens, then divided into subgroups each of six mices and following up (3,7, days intervals post infection process). P.aeruginosa burned mice group, shown skin infection more progressive and more severity in comporning with group infected by Staph. aureus, In addition, they were significant increases in the body temperature of all infection burned mice group(p.value)compared with those pre - infection process. Reisolation of bacteria from infection mice organs revealed, that high percentages in experimental p.aeruginosa burned model comparison with Staph. aureus.Diagnosed bacterial isolates by API 20and Vitek compact system, in addition to molecular diagnostics using PCR technique for the detection of 16S rRNA gene, All isolates showed a positive result of this gene. The amplified 16S rRNA gene seguence was compared with the seguence in NCBI seguence database.the bacterial strain identified as P.aeruginosa EPSI. This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial identification; various bacterial habitats are surveyed to compile their species compositions.
