تحديد الصفات المظهرية والوراثية لانواع بكتريا المكورات المعوية المعزولة من المرضى في محافظة النجف الاشرف == Phenotypic And Genotypic Detection of Enterococcus Sp. Isolated From Patients In Al - Najaf Al - Ashraf Governorate
Author name:
زهراء حميد عودة القريشي
Supervisor name:
مهدي حسين محيل العمار
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2666 - p.pdf
Abstract:
The study aimed to isolate and identify the Enterococcus spp. from different clinical specimens and study the virulence factors of predominant species, as well as detected the virulence factors encoding genes such as efaA (endocarditis - associated antigen), esp (enterococcal surface protein), eep (stimulating of pheromones expression) and enlA (enterolysin A) genes by PCR techniques.There were three hundred clinical specimens collected from patients suffering from different clinical infections during the period from September 2013 to January 2014 in AL - Sadder Medical City and AL - Hakem General Hospital. The identification of the Enterococcus spp. isolates were depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed with the automated VITEK - 2 compact system using Gram positive - Identification (GP - ID) cards.According to the results obtained by the VITEK tests forty two clinical isolates of Enterococcus were detected, which distributed into : (22) isolates from urine, (8) vaginal swabs, (6) seminal fluid, (4) throat swabs and two isolates from wound swabs with no isolates from cerebral spinal fluid, stool and blood specimens.This study revealed that the E. faecalis is more distributed with 25(59.52%), followed by E. faecium with 10 (23.80%), E.avium with 5 (11.90 %), E. durans and E. raffinosus with 1(2.39%) for each.The study investigated the virulence factors of E. faecalis, E.faecium and E.avium, which play a major role in enterococcus pathogenicity. E. faecalis and E.faecium had the ability to producecapsule, gelatinase, biofilm, adhesion, protease, bacteriocin, haemolysin and cytolysin except ? - lactamase produced only by E. faecalis while E.avium produced all these virulence factors except gelatinase, bacteriocin and cytolysin.The results revealed variation in the resistance of bacteria to antibiotics, E.faecalis express absolute resistance (100%) to Erythromycin, high resistance against Gentamycin, Tetracyclin and Vancomycin but high susceptibility to Ciproflaxacin and Penicillin and moderate susceptibility to Chloramphenicol. E.faecium exhibited absolute resistance (100%) to Erythromycin, Gentamycin and Tetracyclin, high resistance against Chloramphenicol, Penicillin and Vancomycin but high susceptibility to Ciproflaxacin while E.avium exhibited susceptibility (100%) to all these antibiotics except Tetracyclin showed absolute resistance.Then detected the virulence factors encoding genes : efaA, esp, eep and enlA genes by using PCR techniques and Electrophoresis Systems.Finally, The genotypic method. Regard to genotypic study the outcome showed that 21(84%) isolates of E.faecalis, 4(40%) of E.faecium and 5(100%) of E.avium were carrying efaA gene and 17(68%) of E.faecalis, 5(50%) of E.faecium, 5(100%) of E.avium were carrying esp gene while eep gene was carrying only by E.faecalis and E.faecium ; 12(48%) and 3(30%) respectively. Also the results revealed that only 1(4%) isolates of E.faecalis have enlA gene.
