دراسة لتكوين الغشاء الحيوي لبكتريا Escherichia coli وProteus mirabilis المعزلة من اصابات المسالك البولية في محافظة النجف الاشرف == Study of Biofilm formation of Escherichia coli and Proteus mirabilis isolated from urinary tract infection in AL - Najaf Al - Ashraf governorate
Author name:
رسل عيدان محسن
Supervisor name:
سهام جاسم محسن
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Pathological Analyzes
Language:
Arabic
University location:
Najaf
First pages:
24T3026 - p.pdf
Abstract:
The aim of this study is to inferring the ability of biofilm production and virulence factors which has helped to form it into two types of bacteria P.mirabilis and E.coli. Two hundred and fifty samples have been collected from patients who are suffering Urinary tract infection (UTI) in Teaching Hospital at Al - Najaf Al - Ashraf during the period ( 1/10/2014 - 1/1/ 2015). The samples have has identified by diagnostic methods : culturing , microscopic , biochemical and using VITEK - 2 compact system more sensitively and specifically method.The results have been showed that the isolation ratio was 30 (37.5%) of E.coli, while P.mirabilis was 14(17.5%) also, a five isolates (6.5%) wereEnterobacter cloacae ssp cloacae and only two isolates (2.5%) were Raoultella ornithinolytica from the total number of specimens. The virulence factors and antibiotic sensitivity test of two bacteria : P.mirabilis and E.coli were determined and the more virulence and multidrug resistance were selected.The hemagglutination properties of P.mirabilis which isolated from clinical samples were determined , the reaction with red blood cells of human group O+ was explored. The ratio of Hemagglutination was reached 42.86%, 50%, 7.14% to MSHA, MRHA and NOHA respectively. However, E.coli has been a different results that was reached 30 % , 26.67% and 43.33% from type MSHA, MRHA and NOHA as well. On the other hand, the thin - film production (pellicle) has been formed by 13 isolates of bacteria P.mirabilis and 21 isolates of bacteria E.coli, when it was growth on the nutrient broth in a temperature 37˚C for 24 - 18 hours. Moreover, it has been shown that there is high affinity for adhesion with the epithelium cell in the urinary tract. In addition, the E.coli has proved the higher efficiency than P.mirabilis in adhesion. It was detected ability of bacteria to biofilm production in three methods. The bacteria E.coli have showed their ability on biofilm production where by the rate was (43.3, 52 and60)% on the different growth ways which was the Congo Red, the tube and the plate respectively.The gel electrophoresis has been done on all isolates which was to analyzed on adhesive factors genetically whereby the bacteria P.mirabilis, E.coli have shown ability to gene expression (fimH and afaB) with a rate of (42.86 and 28.57)% and (30 and 20)%, respectively.Also, it has been clarified that the antibiotics ( Ciprofloxacin, Amikacin and Ceftazidime) have an effect on the most virulent bacteria to prevent the formation of biofilm when it is taken a half of minimum inhibitory concentrations. It was found that the minimum inhibitory concentrations were the most efficient in the prevent a biofilm formation in both types of bacteria.It appears to be found that the minimum inhibitory concentrations of these antibiotics were the most efficient in the prevent the formation of biofilm in both types of bacteria. Ciprofloxacin has the high efficiency to prevent the biofilm formation in E.coli while this concentration has no effective on biofilm production from others. It has been found that there is a clear positive relationship between the presence of adhesion gene (fimH , afaB) and the ability to adhesion on the epithelial .The conjugation process was conducted successfully to transfer the adhesion genes between (E.coli and P.mirabilis) which have been considered a donor strains to transfer the genes to the standard strain E.coli HB101 which have been considered a receipt strains whereby have shown ability to adhesion and biofilm formation.
