دراسة مظهرية ووراثية عن بكتريا Enterococcus faecalis المعزولة من مصادر سريرية مختلفة في مدينة الديوانية == Phenotypic And Genotypic Study On Enterococcus Faecalis Isolated From Different Clinical Sources In Al - Diwaniyah City
Author name:
حيدر سعود مايح الكرعاوي
Supervisor name:
ازهار نوري حسين الموسوي
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Qadisiyah
First pages:
24T2831 - p.pdf
Abstract:
جمعت 280 عينة من حالات سريرية مختلفة من مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية للفترة من 20/11/2012 لغاية 12/4/2013، لغرض عزل وتشخيص المكورات المعوية البرازية Enterococcus faecalis ودراستها مظهريا ووراثيا. اذ عزلت وشخصت 20 ع | Two hundred eighty samples were collected from different clinical cases of Diwaniyah Educational Hospital and Maternity and Children Hospital in the city of Diwaniyah, during the period from 20/11/2012 till 12/04/2013. 20 isolates of Enterococcus faecalis were isolated and identified.This isolates distributed on : 10 isolates from urinary tract infections and 10 isolates from cases of diarrhea while not isolated from wound and burn infections. The results of morphological cultures and microscopic study, biochemical tests, In addition to the use of diagnostic system Histrep and then the final diagnosis by a Vitek 2. Molecular diagnosis showed that all isolates contained 16S rRNA gene. Susceptibility test was done for all bacterial isolates against (9) antibiotics. The results showed that all isolates were complete resistance (100%) against Erythromycin, Tetracycline, Rifampin and Ciprofloxacin. While its resistance for other antibiotics was varied. The resistance isolates for Chloramphenicol, Nitrofurantoin, Penicillin, Gentamycin and Vancomycin were 50 %, 85%, 60 %, 70 % and 35 %, respectively. The results showed that the total resistance for E.faecalis isolates was higher than the sensitivity (more than 70% of these antibiotics). Polymerase chain reaction was used for detecting some genes for antibiotics resistance. The results showed that 7 bacterial isolates had van gene which were resistance to Vancomycin. 6 isolates of these bacteria contained vanB gene while one isolate contained vanA gene. Also the results showed that the bacterial isolates had antibiotics resistance genes as follow : aac (6) - aph (2) gene responsible for resistance to Gentamycin, cat gene responsible for resistance to Chloramphenicol, erm (A)gene responsible for resistance to Erythromycin, and tet (M) gene responsible for resistance to Tetracycline were 70%, 50%, 45%, 100% respectively. Some virulence factors had been detected for bacterial isolates including the ability of isolates of haemolysin production, protease production, gelatinase production and the ability to capsule formation. The results showed that 5 isolates (25 %) its ability of producing was type beta, and 15 isolates(75%) its ability of producing protease, 8 (40%) its ability of producing the gelatinase , and 5 isolates (25%) had the ability on capsule formation. The investigation for these virulence factors in genotypic method, the results showed that bacterial isolates had cylA gene responsible for the production of haemolysin, asal gene responsible for the production of aggregation substance, esp gene responsible for the production of Surface proteins for Enterococcus faecalis and hyl gene responsible for the production of hyaluronidase the percentage 25%, 90%, 55% and 5% respectively.
