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دراسة وبائية وجزيئية للجرثومة Escherichia coli O157 : H7 المعزولة من عينات سريرية وبيئية في محافظة البصرة - العراق == Epidemiological and molecular study of the bacterium Escherichia coli O157 : H7 isolated from clinical and environmental samples Basra Governorate / Iraq
Author name:
عباس ضرب شعبان
Supervisor name:
ميثم ايوب عبد القادر الحمداني
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University Of Basrah - College Of Science - Department Of Biology
Language:
Arabic
University location:
Basrah
First pages:
24T3559 - p.pdf
Abstract:
A total of 464 clinical and environmental samples attempt to isolate Escherechia coli O157 : H7 serotype were collected, of which 183 stool samples from diarrheal children at Al - Qurna hospital and Al - Medaina Hospital of children during the period between October 2013 - August 2014. Environmental samples (281) : 121 samples of drinking ( tap) water , 80 samples of river water , and 80 samples of sewage water (collected from different regions of Basra Governorate) . All samples was tested and detected the presence of Escherichia coli serotype O157 : H7 using chromogen agar and ( CT - SMA ) as selective media and using direct immunological testing for direct identification of the pathogen (Certeste) for clinical samples.The study revealed the isolation of 56 isolates of E.coli O157 : H7 at a rate of (12.06%) : 16 clinical isolates at a rate of (8.7%), 40 environmental isolates at a rate of (14.23) : 9 isolates from drinking water at a rate of (7.4%), 18 isolation from sewage at a rate of (22.5), and 13 river isolates at a rate of (16.25).Antibiotic susceptibility testing toward 20 antibiotic of E.coli O157 : H7 isolates showed that all isolates from all sources resistant to the antibiotics Ampicillin, Amoxacilllin, , Rifampin and Piperacillin while showed high sensitivity to antibiotics amikacin, Kanamcin, ciprofloxacin and norfloxacin.The clinical isolates of E.coli O157 : H7 showed multiple high antibiotic resistance index Muliple Antibiotics Resistance (MAR index) ranged between (0.25 - 0.8), Where two isolates showed a resistance to sixteen antibiotics, Two isolates showed resistance to twelve antibiotics, Two isolates showed a resistance to eleven antibiotics, one isolate showed a resistance to ten antibiotics,Three isolates showed a resistant to nine antibiotics and two isolates showed aresistance to eight antibiotics, One isolate showed a resistant to six antibiotics and two isolates showed resistance to five antibiotics.The environmental isolates of E.coli O157 : H7 showed less resistance ranged between (0.25 - 0.75) for samples of sewage and (0.35 - 0.50)rivers samples , and (0.30 - 0.55)tap water samples . As shown, one isolate resistant to fifteen antibiotics, one isolate showed a resistance to thirteen antibiotics and four isolates showed a resistance to ten antibiotics from sewage. As for rivers Tow isolates showed a resistance to ten antibiotics, three isolates showed a resistant to nine antibiotics while a one isolate of tap water showed a resistance to eleven antibiotics, three isolates showed a resistance to ten antibiotics and an one isolates showed a resistance to nine antibiotics and two isolates resistant to eight antibiotics.Investigating about the content of plasmid in isolates E.coli O157 : H7 to study the relationship between the plasmid content as an indicator of antibiotic resistance, as the results showed that most of the isolates E.coli O157 : H7 four clinical isolates containing the plasmid including one molecular weight > 10kbp with a Patterns of multiple different resistance,also two isolates contain three plasmids of a molecular weight > 10kb. And one isolate containing four plasmids with molecular weights ranging from (4 - 10Kb) and one isolate containing five plasmids resistant to sixteen antibiotics with molecule weights ranged between >(2 - 10Kb).The isolates E.coli O157 : H7 isolated from the environment , sixteen isolates showed that they contain plasmids of molecular weights> 10kb , one isolate contains two plasmids of molecular weights ranging between (4 - 10Kb), five isolates containing three plasmids of molecular weights ranging from (2.9 - 10) Kb and two isolates containing four plasmids of molecular weights ranging between( 2 - 10 Kb
