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دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province
Author name:
محمد خضير عباس النعيمي
Supervisor name:
عباس عبود فرحان الدليمي
General topic:
Biology
Specific topic:
Microbiology - Bacteria
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Diyala
First pages:
24T2690 - p.pdf
Abstract:
شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.
