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تاثير تراكيز مختلفة من الجبرلين والسماد النتروجيني في نمو وحاصل والمادة الفعالة لصنفين من نبات الكرفس Apium graveolens L. Effect of Use Different Concentration of Gibberellic Acid And Nitrogen Fertilizer On The Growth, Yield And Active Substance For Two Cultivars of Celery

المستخلص: نفذت التجربة في الموسم الشتوي لعام (2013 - 2014) م في اصص بلاستيكية بقياس 15×16 سم في محافظة ذي قار, لمعرفة تاثير الرش بحامض الجبرلين ومستوى السماد النتروجيني والتداخل بينهما في النمو ومحتوى الثمار من الزيت الطيار والمادة الفعالة لصنفين من نبات الكرفس Api | The experiment was conducted in the winter season of the year (2013 - 2014) A.C. in a home garden in the governorate of Dhi Qar, to find out the effect of spraying Gibberellic acid and level of nitrogen fertilizer and the interference between them in the growth and content of the fruits of the essential oil and active substances for two cultivars of Celery (Apium graveolens L.).The design of The experiment was Randomized Complete Blocks Design (RCBD) with three replicates in factorial arrangement for three factors (2×4×4 the first factor includes two types of Celery cultivars (local and imported), the second factor includes four concentrations of Gibberellic acid (0, 50, 100 and 150) mg.L - 1, the third one includes four levels of nitrogen fertilizer (0, 10, 20 and 30) kg.dunam - 1. used Revised Least Significant Difference (RLSD) at probability level of (0.05) to compared the averages. The results showed the following : 1 - The local cultivars was significantly Outweighed in content of chlorophyll a, b and total chlorophyll and the percentage of essential oil in plant seeds. imported cultivars was significantly Outweighed on the local cultivars in other characters. 2 - The applying of Gibberellic acid caused a significant increase in all traits, the highest percentage increasing of the Characteristics at a concentration of Gibberellic acid (150) mg.L - 1 for most traits.3 - The nitrogen fertilizer levels (30)) kg.dunam - 1 were used gives significant increase in all of studied traits of the plant except for the percentage of phosphorus, where the increase did not reach to the significant level.4 - The interference between cultivars of plant and Gibberellic acid showed a significant difference in most of the studied traits except the Characteristics stem diameter, number of branches, flowering inflorescences, the wet and dry weights for shoot and root increase in significantly. the treatment of (150)) mg.L - 1 with a imported cultivar was Outweighed significantly in most vegetative growth Characteristics.5 - Binary interference between the plant cultivars and nitrogen fertilizer showed a significant effect for most of the characteristics, which reached the highest increase significantly treatment (30) Kg.dunm - 1, nitrogen of the imported cultivars. 6 - The binary interference between Gibberellic acid and nitrogen fertilizer caused significant effect in all traits except number of branches. Outperforming combination plants (150) mg.L - 1, Gibberellin and (30) Kgm.dunm - 1, significantly nitrogen fertilizer in most vegetative growth Characteristics and floral characteristics and the percentage of oil and active substances and yields the plant from seeds and t1000 seed weight.7 - The triple interference between study factors significantly affected the most qualities, where the given combination (150) mg.L - 1, Gibberellin and (30) Kg.dunm - 1, fertilizer nitrogen in the imported cultivars highest significant increase in most vegetative growth Characteristics and yields the plant from seeds.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: انتصار حسين مهدي
اسم المؤلف: ماهر جاسم محمد الاسدي

دراسة عدد من المتغيرات الفسلجية والكيموحيوية المؤثرة في حصى الكلى لدى المرضى في مدينة تكريت وضواحيها A Study of Number of Physiological And Biochemical Effectual of Renal CALCULI in Patients From Tikrit City And It's Suburbs

المستخلص: جرت هذه الدراسة في مستشفى تكريت التعليمي في محافظة صلاح الدين للفترة من تموز (2007) ولغاية كانون الثاني (2008) والتي تهدف لدراسة عدد من المتغيرات الفسلجية والكيموحيوية المؤثرة في حصى الكلى لدى المرضى في مدينة تكريت وضواحيها وتم قياس تركيز هرمون الباراثارم | This Study had been carried in Training Tikirt hospital Salah El - din governorate during the period of July (2007) up to December (2008). This Study aimed to study number of Physiological and Biochemical effectual of renal calcul in Patients from Tikrit City and it's Suburbs and the concentrations of Parathrmone (PTH) and Calcitonin (CT) had been determind and Number of electrolites Calcium (Ca++), Magnesium (Mg++), Phosphorus (P+++) and Uric Acid concentrations and another blood parametrs had been determind too count of White Blood Cell (WBCs) and averge of Erythrocytes Sedimentation Rate (ESR). Sixty patients and Thirty healthy male and female were selected.The total number of patients were (25, 35) femal and male while the total number of healthy were (13, 17). The avarege of their ages were (20 - 80) year.The Medical Staff in Training Tikirt hospital diagnozed the patients and healthyby depending on the Clinical, Lab and Ultra Sonard reports.The results showed that : 1 - There was no significantly increased among patients and healthy at the levels of Hormone (PTH) and (Ca++), While the patients were significantly increased (P<0.05), (P<0.01) comparing with the healthy at the levels of (P+++), (UA), (WBCs) and (ESR). There was significantly decreased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) in patients comparatively with healthy.2 - The female patients were significantly increased (P<0.05), (P<0.01) at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR) comparing with healthy females while the patients male were significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT), (UA), (WBCs) and (ESR). Healthy males were significantly increased (P<0.01) (Mg++) comparing with patients males and also the healthy females were significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) comparing with patient females.3 - The patients were increased in their aged group (20 - 39) year comparing with healthy significantly (P<0.05), (P<0.01) at the levels of (P+++), (UA), (WBCs) and (ESR), while the healthy who were increased in this group significantly (P<0.01) at the level of (Mg++) comparing with patients. According to the group of (40 - 59) year we noticed asignificantly increased (P<0.05) patients comparing with healthy at the levels of (P+++), (UA) and (ESR). The healthy were significantly increased in this group (P<0.05) at the level of (Mg++) comparing with patients. In the group (60 - more) year the patients were significantly increased (P<0.05) comparing with healthy at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR). While the healthy were increased in this group significantly (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) comparing with patients.4 - The patients were significantly increased (P<0.05) in mass group(20 - 25) Kg/m2 comparing with healthy at the levels of (P+++), (UA), (WBCs) and (ESR). While the healthy were increased In this group significantly (P<0.05) at the levels of hormone (CT) and (Mg++) comparing with the patients. In the mass group (31 - more) Kg/m2 significantly increased (P<0.05), (P<0.01) at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR) comparing with healthy. The healthy achieved significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++).5 - According to the place of living we noticed asignificantly increased (P<0.05), (P<0.01) for patients who were living in cities at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy and they increased at the level of (Mg++). While the patients who were living in district were significantly increased (P<0.05) at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy. The patients who were living in village were significantly increased (P<0.05) at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy while the healthy achieved significantly increased (P<0.05) at the levels of hormone (CT) and (Mg++) comparing with patients.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: وهبي عبد القادر سلمان نهاد نجرس هلال
اسم المؤلف: حلا حميد مجيد جاسم

دراسة وبائية لطفيليات القناة الهضمية عن منطقتي ابو غريب والعامرية وتاثيرها في بعض مستويات الدم Survey of Intestinal Parasites In Abu - Ghreeb And Al - Ameria Areas And Their Effect On Some Blood Components A Thesis Submitted

المستخلص: تضمنت الدراسة الحالية اجراء مقارنة حول نسب انتشار الطفيليات المعوية وعلاقة الاصابة ببعض مكونات الدم المحيطي (اقيام الهيموغلوبين والعدد الكلي لكريات الدم البيض واعداد الحمضات) في مرضى من منطقتي ابو غريب والعامرية خلال المدة مابين شهر تشرين الاول 2011 ولغاي | The present study has included the comparison 0f prevalence rates of intestinal parasites and the relationship of infection of certain components peripheral blood (hemoglobin values, the total number of white blood cells, eosinophils numbers) in patients of each of the regions of Abu Ghraib and Amiriyah during the period from October, 2011 until July, 2012 as it has been collected (2449) stool samples that were distributed between (1430) stool samples from Abu Ghraib Hospital auditors and (1019) stool samples of auditors of the Health Center in Amiriya and also it has been collected blood samples. Stool samples were examined by direct method using brine (Normal Saline) and tincture of iodine (Lugol's Iodine) and the floating way by sulphate zinc water (ZnSo4.7H2O) to investigate the prevalence of intestinal parasites and the relationship of infection by age, sex, number of family members, and the type of water used for drinking. The results showed the presence of significant difference at the possibility(p<0.05) in total infection for the regions of Abu Ghraib and Ameriyah where the percentage was (36.29%) in the area of Abu Ghraib and (27.57%) for the Amiriyah area. It has been diagnosed five types of intestinal parasites (protozoa + Helminthes) as follows : A - Intestinal protozoa in the Abu Ghraib and for the Amiriyah area : 1 - Entamoeba histolytica was (17.62%, 16.68%).2 - Giardia lamblia was (11.95%, 5.49%).3 - Entamoeba coli colon was (2.44%, 2.55%(.B - Helminthes1 - Dwarf tapeworm Hymenolepis nana was (2.37%, 1.76.(2 - pinworm Enterobius vermicularis was (1.88%, 1.07.(We Did not observe any significant differences between the infection of intestinal parasites and sex factor where the total infection for males was (6.53%) while for females, it reached (8.06%) for Abu Ghraib, as to the Amiriya region, also we did not notice any significant differences between the infection of intestinal parasites and sex factor where The total infection for males was (7.26%) while for females, it was (6.84%(. It has been also found that there are significant differences and when probability of (P <0.05) for the infection with parasite (Giardia lamblia) between the areas of Abu Ghraib and Amiriyah where infection rate reached (11.9%) in Abu Ghraib while for the Amiriyah area, it has reached the ratio of, (5.49%) for the same parasite, while there was no significant difference between the other four types of parasites. The individual injuries are prevalent Lama has reached bilateral injuries (56) bilateral injury in the Abu Ghraib area, and (13) in the area of bilateral injury Ameria and most of the injuries were bilateral parasite (E.histolytica+ G.lamblia). The results showed that there is a significant difference when probability (P <0.01) for the distribution of age groups, reaching the highest infection rate in the age group of (29 - 20) years, (56.68%) and the lowest infection in the age group (60 years and over) the percentage of infection was (21.42%) in the Abu Ghraib area, while for Amiriyah area, it has been recorded the highest infection rate in the age group of (29.20), where it reached (49.22%) and the lowest injury in the age group (60 years and above) where The percentage of infection was (4.82%(. Also, it was noticed the emergence of variation in the values of the components of the peripheral blood and the lack of significant difference when probability (P <0.05) in the area of Abu Ghraib where it recorded the highest proportion of hemoglobin in the parasite (Entamoeba histolytica), amounting to (12.40 g / 100 ml) and the lowest percentage hemoglobin was recorded in parasite (Enterobius vermicularis) amounting to (9.46 cells / mm3), and the lack of a significant difference in the total number of white blood cells where it recorded the highest rate in the parasite (Entamoeba histolytica) 6776.32 cells / mm3 while the lowest rate was recorded in the parasite (Enterobius vermicularis (5074.37cell\mm3, and lack of a significant difference for eosinophile numbers was recorded the highest proportion in parasite Hymenolepis nana 516.63 cell\mm3 and the lowst rate was recorded in parasite Entamoeba histolytica 159.63 cell\mm3. As for the Amiriyah, it hasn’t been noticed any significant differences when probability (P <0.05), where the highest proportion of hemoglobin in the parasite (Entamoeba histolytica), was (11.76 g / 100 ml) and the lowest percentage hemoglobin recorded in parasite (Enterobius vermicularis) was (9.90 g / 100ml), and there was the lack of a significant difference in the total number of white blood cells, where it has been recorded the highest rate in the parasite (Entamoeba histolytica) 6574.59 cells / mm3 and the lowest rate was recorded in the parasite (Entamoeba coli) 5700.00 cells / mm3 and the lack of a significant difference for eosinophile numbers. The highest proportion in parasite Enterobius vermicularis was 518.65 cell\mm3 and the lowst rate in parasite Entamoeba coli was 140.29 cell\mm3. The results also showed that there is a high significant difference when the probability (P <0.01) in the incidence of intestinal parasites and the relationship of water used for drinking where the study proved that the highest infection rates recorded of the people who use canal waters and water tank where It percentages was (49.54%, 39.00%), respectively while the lowest rate of infection was recorded in people who use boiling water which reached (14.02%). The same applies to the Amiriya district which recorded the highest percentage of people who use tap water and the lowest percentage of those using boiling water reaching ratios (41.49 %, 12.5%), respectively.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: طالب عبد الله حسين
اسم المؤلف: دعاء بهاء عبد الجبار

دراسة وبائيــة وتشخيصيـة للانماط الوراثية لطفيلي Giardia lamblia المسبب للاسهال لدى المرضى في محافـــظة النجــــف الاشرف باستخدام تقنيـــة الـ PCR Epidemiological And Diagnostic Study of The Giardia Lamblia Parasite Genotypes, Which Causes Diarrhea Among The Patients In Al - Najaf Al - Asharf, Province, By Using The Pcr Technique

المستخلص: اجريت الدراسة الحالية في قسم علوم الحياة - كلية التربية للبنات - جامعة الكوفة للمدة من تموز 2011 ولغاية حزيران 2012 والتي تهدف الى دراسة وبائية طفيلي Giardia lamblia وتحديد الانماط الوراثية للطفيلي لدى المرضى المصابين بالاسهال في محافظة النجف الاشرف باستع | The Present study was carried out in the Department of Biology - College of Education for Girls - University of Kufa for the period from July 2011 until June 2012, it aims to study the prevalence of the Giardia lamblia parasite and identify genotypes of the parasite in patients with diarrhea in the province of Najaf by using the microscopic examination and the polymerase chain reaction (PCR).The results of microscopic examination of 3383 stool samples, 500 samples are infected by the G. lamblia parasite, with a total percentage of 14.8%. The results of the statistical analysis showed that there were significant differences at the level of probability P ? 0.05 where the rate of infection in the liquid samples was higher than it is in semi - liquid samples, reaching 17.2% and 10.7% respectively. As the results show the incidence of the parasite in males are higher than it in females reached 16.8% and 11.1%, respectively, as well as the spread of infection with the parasite in the rural areas more than the urban area where the ratio was 19.6% and 9.1%, respectively, the results also show that the highest infection rate with the parasite was in the age group aged (1ess than year) and the lowest in the group aged (36 - 40) years, reaching 28.0% and 10.0%, respectively. The results also show that the highest percentage of parasite infection was in the September, 44%, while the lowest infection rate was in December, reaching 6.1%. The results of the current study show that the highest infection rate parasite was in patients in the hospital of Manathira which amounted to 16.5% and that the lower infection rate was among patients of the Medical Al - Sader city hospital, reaching 11.7%. The results of extracting (100) Stool Samples by microscopic examination for patients infected with G.lamblia showed the presence of the gentic material DNA of the G. lamblia parasite in (41) samples which constitute 41%, and results of the examination by PCR by using Triose phosphate isomerase (Tpi) Showed that there are signiinficant differences at the level of probability, p ? 0.05 percentage infection with the genotype (B) compared with the genotype (A), which were 61% and 39.1% respectively.The highest rate of infection with the genotype (B) in the semi - liquid samples was 69.3% compared to the liquid samples, which amounted to 67.9%, while the genotype (A) had the highest rate of infection in the liquid samples, which amounted to 32.2% while it was 30.8% in the semi - liquid samples.As the results showed that the percentage of infected male with the genotype (B) was more than it is in females, reaching 62.5% and 58.9%, respectively, and in type (A) the infection of females was higher than the infection males 41.2% and 37.5% respectively. As the results show the spread of genotype (B) in the urban area is more than the rural area, the percentage of infection is, 72.8% and 66.7% respectively, while type (A) was more prevalent in rural than in the urban, where the percentage of infection is 33.4% and 27.3% respectively.The results also showed that the highest incidence of the genotype (B) was in the age group (41 - 45), it reached 75% and the lowest percentage in the age group(1 - 5) (11 - 15) (26 - 30) (36 - 40) years, amounting to 50%, while genotype (A), the highest rate of infection, when age group (1 - 5)(11 - 15)(26 - 30)(36 - 40) years to approximately 50% and the lowest percentage of infection in the age group (41 - 45), was 25%.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: جميل جري يوسف الحميداوي
اسم المؤلف: وداد هاشم يحيـى ناصر المحنة

دراسة تشخيصية لانواع تحت العائلة Tubificinae (قليلة الاهلاب : نايديدي) من بيئات مائية مختلفة داخل مدينة بغداد - العراق An Identification Study On Subfamily Tubificinae (Oligochaeta : Naididae) From Different Aquatic Habitat In Baghdad / Iraq

المستخلص: انواع تحت العائلة Tubificinae ديدان مائية حمراء اللون يتراوح طولها مابين 1سم الى عدة سنتمترات وقطرها حوالي 1ملم تنتمي الى العائلة Naidiadae، الصنف الثانوي قليلة الاهلاب، صنف السرجيات التابع لشعبة الديدان الحلقية، وتسمى عاميا" بديدان الوحل. تقطن هذه المجم | Species of Subfamily Tubificinae are aquatic red worms, their length ranged between 1cm to several centimeters, and their diameter about I mm, and they are classified with Family Naididae, subclass Oligochaeta, class Clitellata, phylum Annelida. They are commonly called "sludge worms". This group inhabit the rivers, lakes and ponds sediment, and some species also found in marine habitat, they are considered as one of the main benthos components specially in freshwater, with increase abundance in soft sediment rich in organic matters. Sludge worms individuals are hermaphrodite, reproduced sexually by cross - fertilization, eggs led in cocoon and hatched directly without any larval stage. They are also reproducing asexually by ArchitomyAccording to the economic importance of this worms as good food for fish, their ecological role as well known bio - indicators, little studies about their identification were available and absence of check list of their species in Iraq, the present investigation idea was come to concern primarily with sorting and identification of subfamily Tubificinae from River Tigris and some water surface in Baghdad /Iraq. For this purpose six study sites were chosen, including a drainage canal, North Baghdad (S1); three sites on the shore of River Tigris (S2, S3 & S4) ; in addition to site in Al - Jeish canal, East Baghdad (S5), finally the sixth site was from pond in Al - Zawra'a park, within the center of Baghdad (S6). These sites were characterized by sedimenet rang from clay, silty - clay to silty with a percentage of organic matter ranged between higher percentage of 9.9% in site S5 and 0.7% as a lowest percentage in site S1. According to water temperature, salinity and pH values, they were identical in all study sites.3628 individuals were sorted from 24 samples collected from all study sites. The sorting results revealed that the highest number of worms (1346 ind.) recorded in site S5, while the lowest number (326ind.) recorded in site S4. Twelve species were identified belonging to seven genera, and Limnodrilus was the most dominant genus. Five species were considered as new records to Iraq, which are L. silvani in S6, P. hammoniensis & Monopylophorus irroratus in P. moravicus in S2 and Rhyacodrilus cocciensis in S6.Five species of Limnodrilus were recorded, including L. hoffmeisteri, L. claparadienus, L. profundicola, L. udekemianus, and L. silvani with a percentage of 52%, 1.40%, 4%, 10.03% and 0.35% for each species respectively. Two species of Potamothrix were recorded which are P. bavaricus and P. hammoniensis with a percentage of 2% and 2.05% respectively, in addition to Branchiura sowerbyi, Psammoryctides moravicus, Tubifex tubifes, Rhyacodrilus cocciensis, and Monopylophorus irroratus with a percentage of 26.07%, 0.33%, 3%, 0.27, and 0.24 respectively. The higher frequency percentage of 100% was recorded by L. hoffmeisteri and B. sowerbyi.High densities of Tubificinae were recorded during all study period. Higher density of 6882 ind./m2 was recorded during in site S1; 4296 ind/m2 in site S2 ; 4496 ind/ m2, ind/ m2in site S3; 3730 ind/ m2 in site S4; 15218 ind/ m2 in site S5 and 7026 ind/ m2 in site S6.The results of relative abundance revealed that L. hoffmeisteri was the dominant species in site S3 and abundant in other study sites. B. sowerbyi was abundant in all study sites, while other species were recorded between few to rare species except L. udekemianus which was abundant in site S5, and T. tubifex in site S4. It was clear from species richness index, Shannon - Wiener diversity index, and species uniformity index, that the highest value for each index was recorded during cold months (Dec. - Feb.), 1.47, 2.83bit/ind, and 1.17 respectively, while the result of cluster analysis depending on Jaccard index of similarity revealed that the highest similarity of 80% found between S1& S6.The investigation also includes description of identified species and photos of identification criteria for each species. B. sowerbyi was easily recognized from other species by having gill filaments at the posterior end of the body. Species of Limnodrilus were characterized by the absence of hair chaetae, and they are differentiated by the shape of penis sheath, due to the close similarity of chaetal shapes, with the exception of L. udekemianus in which its anterior chaetae characterized by long and curved dorsal tooth. Species of Potamothrix were recognized by the presence of a pair of spermathecal chaetae, its shape was used to differentiate between P. hammoniensis, which have long gutter - shape and P. bavaricus, which have plade - like shape with triangle base. P. moravicus also have spermathecal chaetae but it was differed from that of Pomatothrix species by its long and narrow shape. T. tubifex was identified by its tub - shaped penis sheath, while R. cocciensis, was recognized by their penial chaetae in segment NO. XI, and M. irroratus by the presence of pseudopenis and its sheath.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: هيفاء جواد جوير
اسم المؤلف: افراح محمد علوان الجبوري

تاثير المستخلص الكحولي الخام لاوراق الجت (Medicago sativa) على بعض المعايير الفسلجية والكيموحيوية في اناث الجرذان المستحثه بمادة الالوكسان Effects of Crude Alcoholic Extract of Alfalfa (Medicago Sativa) Leaves On Some Physiological And Biochemical Parameters In Alloxan Induced Diabetic Female Rats

المستخلص: اجريت هذه الدراسة في مختبر البحوث السريرية في كلية الصيدلة ومختبر فسلجة الحيوان في كلية العلوم / جامعة الكوفة للمدة من 15 / 9 /2011 الى 12 /3 /2012 للتقصي عن فعالية المستخلص الكحولي الخام لاوراق نبات الجت Medicago sativa L. في تخفيض مستوى سكر الدم وبعض ال | This study was conducted at the Clinical researches laboratory/ Pharmacy College and Animal physiology laboratory/ Sciences College / university of Kufa during the period from 15/ 9/ 2011 to 12 / 3/ 2012 to investigate the activity of the crud alcoholic extract of the alfalfa (Medicago sativa L). leaves in affecting the blood glucose level and some physiological and biochemical changes due to induced diabetes mellitus by alloxan (150) mg/kg in female albino rats (Ratus ratus), in comparison with the activity of the reference drugs Glibenclamide. This study was also used to know the effect of the extract in normal (healthy) female rats. In this study (54) female rats were utilized which were divided into (9) groups with 6 rats for each group. Diabetes mellitus was induced in (30) female rats, were distributed into 5 sub groups. One of them was considered as control group for the infected rats, and three groups of them were treated with different concentrations of the extract (250, 500 and 750)mg/kg while the fifth group was treated by the chemical drug Glibenclamide (0.6) mg/kg. The other 24 normal rats were also divided into 4 sub groups, one of them considered as control group. And the other three groups were treated with the extract concentrations (250, 500 and 750)mg/kg. The treating period with the plant extract and the drug continued for 30 day.Results of this study were as the following : ? A significant (P< 0.05) decrease was showen in total body weight, insulin hormone and HDL - c levels, total WBC and RBC count in addition to Hb concentration in infected group of diabetes mellitus in comparison with healthy control group.? A significant (P< 0.05) increase was clear in the levels of glucose, TC, TG, LDL - c, VLDL - c, urea, creatinine and ALT and AST enzymes in infected group in comparison with healthy control group.? The healthy rats that treated with the extract concentrations were showed a significant increase in their total body weight at the end period as compared with the first day of experiment, there was also a significant (P < 0.05) increase in body weights of the infected rats that were treated by the two concentrations (500, 750) mg/kg of extract as compared with infected control group.? The infected rats that treated by all three concentration of plant extract and the glibenclamid drug were exhibited a significant (P< 0.05) increase in insulin level as compared with infected control group.? on the other hand, it was also showen a significant (P< 0.05) decrease in glucose level of infected rats that have been treated by the three concentrations and the (0.6) mg/kg concentration of glibenclamid as compared with infected control group.? The levels of TC, TG, LDL - c, VLDL - c were significantly (P< 0.05) decreased, in contrast a significant increase was occurred in the level of HDL - c in infected rats when they treated by all the concentrations of plant extract as compared with infected control group.? The urea and creatinine levels showed a significant (P< 0.05) decrease when the infected rats were treated by the two concentrations (500, 750) mg/kg as compared with infected control group, while in the healthy rats, the urea level showed a significant (P< 0.05) decrease as compared with healthy control group.? A significant (P < 0.05) decrease was occurred in ALT enzyme level when the infected rats were treated by all extract concentrations and the chemical drug. But AST enzyme level was significantly (P < 0.05) decrease when the infected rats were only treated with the two concentrations (500, 750) mg/kg of plant extract in addition to glibenclamid as compared with healthy control group.? The two concentrations (500, 750) mg/kg of plant extract and the concentration (0.6) mg/kg of the glibenclamid drug caused a significant (P< 0.05) increase in total RBC count, and the hemoglobin concentration, while only the larger dose of plant extract caused a significant (P < 0.05) increase in total WBC count in infected rats as as compared with infected control group.In conclusion, the results of this study indicate that the two concentrations (500, 750) mg/kg of alfalfa leaves extract gave a good results, even better than the reference drug, glibenclamid for treating diabetes mellitus, in addition to ameliorate the metabolic disorders that accompany it.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: عبد الزهرة كاظم محمد علي
اسم المؤلف: حوراء سليم محمد طه بشيبش

تحديد تجمعات Giardia lamblia ومعقد Entamoeba histolytica / Entamoeba dispar بالتشخيص الجزيئي (PCR) للمصابين في تكريت Determination of Giardia Lamblia Assemblages And Entamoeba Histolytica / Entamoeba Dispar Complex By Molecular Diagnosis (Pcr) in Patient in Tikrit

المستخلص: اجريت هذه الدراسة للمدة من كانون الثاني 2011 ولغاية كانون الاول 2011 للمرضى الراقدين والمراجعين لمستشفى تكريت التعليمي وبعض المختبرات الخاصة في مركز المدينة وضواحيها والذين يعانون اعراض مرضية مختلفه ولفئات عمرية مختلفة ترواحت بين اقل من سنة الى 61 سنة، ح | This study was conducted for the period started from January 2011 until December 2011 on inpatients and outpatients of Tikrit educational hospital and some private laboratories at Tikrit city and surrounded suburbs. Symptoms of different diseases among various age groups varies between less than one year to 61 years. The tests used the (ELISA & PCR) and the analysis of the PCR - RFLP to discriminate the infection by assemblage of Giardia lamblia and differentiate between pathogenic amoeba Entamoeba histolytica and nonpathogenic Entamoeba dispar compared with microscopical examination. The result of present study showed that (75) person who represent (37.5%) were infected by Giardia lamblia and (75) person who represent (37.5%) were infected by E.histolytica/E.dispare complex, out of (200) stool samples which have been tested by microscopical examination. Epidemiological study showed no significant difference among the sex for the two species parasites whereas a high percentage for infection recorded in group at age (1? - 10) year for Giardia lamblia and E.histolytica / E.dispar complex reach to (60%, 36%) respectively. Regarding the place of living Giardia lamblia in rural area showed infection rate of (19.5 %), while infection rate by E.histolytica/E.dispar complex reaches (22.5%) in urban area. Statistical analysis shows a high difference of significance according the source of water supply. Tap water showed high incidence for initiating infection which reaches to (93.3%) by G.lamblia and (90%) for E.histolytica/E.dispar complex. For the infection rates and their relationship to the educational level, the results show that the highest rates of infection recorded in non - educated (52%) and (73.3%) of Giardia lamblia and E.histolytica / E.dispar complex, respectively. Depending on the sample texture, the result show that oily diarrhea is more correlate with infection reaches to (33.3%) of Giardia lamblia and the watery diarrhea form 40% by E.histolytica / E.dispar complex, while simultaneous occurrence of both symptom (colic and loss of appetite) are the most symptom synchronized with infection records (44%) for Giardia lamblia and (36%) for E.histolytica / E.dispar complex. The percentage of infection by Giardia Lamblia in this study depends on the test results of the ELISA was (20%), while the percentage of infection E.histolytica/E.dispar complex was (6.7%). Testing has shown sensitivity compared to microscopic examination reaches to (22.6%) and (8%) for both parasite respectively, while the test showed a high specificity which reaches the rate (93.3%) and (100%) for both parasites respectively. Result of PCR showed a percentage of infection by E.dispar about (32%) (64 sample) from the total samples, whereas it does register any cases infection by E.histolytica. By counting the results of the PCR for the three genes (ssu rRNA + TPIA + GDH) they show a total infection by Giardia lamblia which was (10.5%) (21 samples). This study recorded percentage of infection Giardia lamblia depending on the result of the three Genes separately amounted to (4%), (5%), (3%) for (ssu rRNA, TPIA, GDH) respectively. According to the result of the analysis PCR - RFLP for GDH gene by using the enzyme NIaIV, it did not record any infection by Giardia lamblia (assemblage A), whereas (6 samples) recorded (100) by Giardia lamblia (assemblage B), by using the enzyme Rsal to determine the secondary assemblage shows that the total (6 samples) infected there are 5 samples carrying infection by two type of secondary assemblage returning to the assemblage B (assemblage BIII +BIV) and only one sample infected with (assemblage BIV). To determine the assemblage and secondary assemblage for the parasite result compared with amplification of TPIA gene with lysis by enzyme for the resulting amplification for GDH gene, resulting a ratio of (14.3%) for a sample infection by both assemblage (A+B) and secondary assemblage (BIII+BIV) together, and a ratio of (57.14%) infection by assemblage A only and (21.4%) infection by secondary assemblage (BIII+BIV) together and (7.14%) infection by BIV only.
الصفحات الاولى:
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الاحياء المجهرية
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: شهاب احمد محمد الجبوري عقيل حسين العاصي
اسم المؤلف: انتصار غانم عبد الوهاب الصميدعي

تاثير اضافة الكالسيوم للتربة والرش بالبورون في صفات نمو وحاصل الحنطة Triticum aestivum L Effect of Added Calcium For The Soil And Spray With Boron In Growth And Yield of Wheat (Triticum Aestivum L.)

المستخلص: اجريت هذه الدراسة في المشتل التابع لمديرية زراعة محافظة ديالى للموسم الشتوي 2012 - 2013 وذلك لدراسة تاثير تراكيز مختلفة من البورون(0, 10, 20, 30, 40 ppm) والكالسيوم(0, 50, 100, 150, 200غم)والتداخل بينهما, بدراسة بعض المعالم المظهرية والفسلجية كارتفاع النب | This experiment did in the nursery which followed for the Diyala Directorate of agricultural in winter season 2012 - 2013, that to study the effectives of different concentrations from Boron (40, 30, 20, 10, 0 ppm ) and Calcium(200, 150, 100, 50, 0) and the interior between them, by using some of appearances and felsitic as a plant arise. The leaf's area ear's length, the greenGather of the dry weight, the contain of the plant with chlorophyll, protein carbohydrate, elements, grain's weight, Brolin. This results cleared that improvement in plant's rising. The leaf's area, ear's length, dry weight, chlorophyllProtein, grain's weight when adding the Boron in concentrations (ppm 10)The rate of increasing(%5.1, 19.7, 47.2, 11.0, 9.7, 44.3, 65) for the specification above. on a continually comparative with controlling equal. But some specifications was reduced above in Comparative (ppm40) the rate of decrease(%2.9, 13.5, 22.1, 6.7, 5.4, 22.6, 39.9) on a continually, byAdding Calcium increased each of plant's rises, The leaf's area, ear's length, dryWeight, chlorophyll, protein and grain's weight in concentrations (50) theIncreasing rate is(%5.6, 17.6, 43.4, 12.1, 10.0, 41.7, 62.9) for the specification above comparative with controllingtreatment, but the specifications reduced in concentrations(200).The rateof reducing is(%2.6, 12.8, 27.5, 6.1, 5.3, 26.5, 45.3).So noticed the Positives increased in contains ofCarbohydrate, prolin, and content of elements that a highest rate of concentrations(200, 150) about the effective of interior that reached a highest rate in level(ppm Bo, 10+Ca 150) so as(ppm Bo 30+ Ca150).
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: محمود شاكر رشيد الجبوري
اسم المؤلف: خمائل علي كريم

دراسـة تصنيفية للجـنـس Rhagadiolus Scop. (Compositae) في العراق A Systematic Study of The Genus Rhagadiolus Scop. (Compositae) In Iraq

المستخلص: تناولت الدراسة الحالية دراسة اربعة انواع للجنس Rhagadiolus Scop. في العراق دراسة تصنيفية مقارنة شملت الصفات المظهرية والتشريحية وحبوب اللقاح والبيئة والتوزيع الجغرافي للانواع اضافة الى التصنيف العددي.شملت الدراسة المظهرية صفات الجذور والسيقان والاوراق وا | The genus Rhagadiolus Scop. in Iraq has been systematically studied. Work involving comparative morphology, anatomy, palynology, ecology & geographical distribution and numerical taxonomy has been presented. The morphological study includes studying the characters of root, stems, leaves, inflorescences, phyllaries, flowers, and fruits in addition to indumentums. It has been clear that the characters of stems, leaves, involucres bracts and fruits have a taxonomic importance.Also it has studied some of the micro morphological characters and their taxonomic importances were assessed.The present study showed that the pollen grains of all species were found to be tricolporate with echinate surface & of little taxonomic value. This study also includes characters of the epidermal leaf and stems, indumentums, venation and transverse section of stems and leaves discussion were also included, the taxonomic importance of each character & its variation, it is shown that these characters have an importance assessing morphological characters to isolate these species.Data obtained from herbarium specimens, literature and trips was utilized to elucidate the ecology of the species. Maps of geographical distribution were prepared for all species studied. It has been shown that R.angulosus was the most widely distributed species in the country. The species under current study were numerically treated ; dendrograms and polygonal graphs were drawn for the species of the genus. In conclusion (4) species of the genus Rhagadiolus : R.angulosus, R.edulis, R.hamosus and R.stellatus were recognized for Iraq, taxonomic treatment has been carried out for all Rhagadiolus species and key was designed for them too.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عبد الكريم خضير البيرماني
اسم المؤلف: اوراس عبد السيد مهدي الحسيني

تقييم مستوى بعض الحركيات الخلوية لدى المرضــى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1 Evaluation of The Level of Some Cytokines In Patients With Herpes Simplex Virus Type I (HSV - 1)

المستخلص: هدفت الدراسة الحالية الى تسليط الضوء على مجموعة من المرضى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1(HSV - 1) من خلال تقييم مستوى نوعين من الحركيات الخلوية الموجودة في مصولهم. شملت هذه الحركيات الخلوية الانترلوكين Interleukin - 6(I | The present study aimed to highlight a group of patients infected with herpes simplex virus type I (HSV - 1) by evaluating levels of two types cytokines in sera. These included cytokines interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - ?) has been evaluated using the technique of Enzyme - linked immunosorbent Assay (ELISA), has also been investigating the relationship between these cytokines and the stages of infection compared to the control group. Before that rapid cytologic method has been used to diagnose the virus by detecting the presence of multinucleated giant cells, and then was diagnosed serologically by specific IgM and IgG monoclonal antibodies against Herpes simplex virus - 1. It was also used Radial immunodiffusion method to evaluate levels of total IgM and IgG in patients sera.Sixty four sera samples were collected from patients who are attending to the consultants clinics of Al - Hakeem general hospital and AL - Sader teaching hospital in AL - Najaf AL - Ashraf governorate for the period from October 21/2012 until March 13/2013. These Samples were divided into two groups : (32) sample during primary and recurrent infection, (32) after recovery from infection (latent stage), as well as (16) sample were collected from healthy individuals, who were regarded as acontrol groups.The study reached to the following results : • A significant increment (p<0.05) in sera concentrations of IL - 6 in patients during infection as compared with control groups, but the increment was not significant in sera of patients during latent stage.• TNF - ? levels increased in sera of patient, but this increment was not significant as compared with controls group.• There was no significant increase of Total IgM levels in sera of patients.• Total IgG levels increased significantly in sera of patients during infection and latent stage as compared with control group.• A highly significant positive correlation (r= 0.7, p= 0.00) between TNF - ? and IL - 6 concentration in sera of patients.• There was no significant correlation (p>0.05) between Total IgM levels and (IL - 6 and TNF - ?) in sera of patients with correlation coefficient (r= 0.1, r=0.2) respectively.• Positive significant correlation (r= 0.4, p= 0.02) was found between total IgG levels and IL - 6 in patients during infection, but was no correlation between them in latent stage.• There was no association between age and IL - 6 and TNF - ? levels in sera of patients with HSV - 1.Finally, the elevation of TNF - ? and IL - 6 levels in sera of infected patients with HSV - 1 may be play an important role in pathogenicity of virus, as well as it may limits the viral infection.Furthermore, there are positive correlation between levels of TNF - ? and IL - 6 in sera of patients
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: سهام جاسم الكعبي
اسم المؤلف: حسين علي كاظم

دراسة بعض التاثيرات المرضية للدودة الدبوسية Enterobius vermicularis لدى الاطفال في محافظة النجف The Study of Some Pathologaical Effects of Pin Worm Enterobius Vermicularis Among The Children In Al - Najaf Province

المستخلص: اجريت الدراسة الحالية للمدة من شهر تشرين الثاني 2011 الى شهر تموز 2012, لمعرفة تاثير الدودة الدبوسية Enterobius vermicularis في بعض المعايير الدموية والكيموحيوية والسريرية لدى الاطفال المصابين بالدودة (12 - 4سنة). استخدمت طريقة الشريط اللاصق الاسكتلندي في | The present study was conducted during the period from November 2011 to July 2012, to evaluat the effect of the pin worm Enterobius vermicularis on the Hematological, biochemical and chlinical parameters in infected children (4 - 12 year).This study included 300 patient from both sexes and different ages. The Scotch Cellulose Tape Technique used for the examination of pin worm in children, attended Al - Sader Educational Hospital, Al - Zahraa hospitals and central laboratory in Al - Najaf Al - Ashraff city. The results of the sudy showed a significant decrease was noticed in Hb and PVC in infected person compared with the control group While there was a significant increase in each of the total number of white blood cells and Eosinophils and Neutrophile and a decrease in Lymphocyte, and it did not show any significant difference in the Basophilis and Monocyte in children infected with pin worm compared with the control group.The results of the study showed a significant increase in the levels of Nitric Oxide, Malondialdehyde, it also showed a significant decrease in the levels of Gluthanione in the infected group compared with the control group. The concentration of Copper, Zinc, Magnesium and Vitamine B12 were low in children infected with pin worm compared with the control group. The high incidence rate of infection accompined with patient suffering from pruritus ani and nocturnal enuresis. The age group (10 - 12) year represent the highest group infected with the worm.The education level influence the distribution of these worm among un educated families, and ratio of infection increase in male and female in families which contain more than (10) members with significant difference P<0.05 and this ratio decreased in children in families which contain between 3 - 4 members.This study did not show any significance defferent in infection among the ratio of infection between male and female under P<0.05.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: هيثم محمد حمادي العوادي
اسم المؤلف: ساهرة عايد عبد الصاحب الموسوي

دراسة التاثير المثبط لبعض انواع بكتريا حامض اللاكتيك ضد بعض انواع الفطريات الممرضة والمنتجة للسموم Study The Inhibition Effect of Some Species of Lactic Acid Bacteria Against Some Pathogenic And Toxins Producing Fungi Types

المستخلص: اجريت الدراسة في مختبرات قسم علوم الحياة في كلية التربية بهدف عزل وتشخيص بعض انواع بكتريا حامض اللاكتيك ومحاولة اختبار قابلية خلاياها او النواتج الايضية منها وكذلك دراسة تاثير المستويات المختلفة من درجة الحرارة والاس الهيدروجيني في القابليـة التثبيطية لب | This work aimed to investigate the effects of some lactic acid bacteria (LAB) species (cells or their metabolic products) at different temperatures and pH levels on some soil pathogenic or toxin produced fungi species. Six LAB isolates and the identification was depended on the morphological and cultural characterized and biochemical testes, the isolates were determined as Lactobacillus delubricii subsp. delubricii, Lactobacillus delubricii subsp. bulgaricus, Lactobcillus casei, Lactobacillus acidophilus, Leuconostoc mesentroides and Streptococcus thermophilus, and four fungal species Microsporum canis, Trichophyton mentagrophytes, Aspergillus fumigatus and Rhizopus stolinfer were identified. In this study the highest inhibition effect against the above mentioned fungi was shown by L. acidophilus as a significant (P< 0.05) efficacy where the diameter of inhibition zone in case of M. canis, T. mentagrophytes, A. fumigtus and R. stolonifer was 26, 32, 30 and 28 mm respectively. Less inhibition activity was exhibited by Leu. mesentroides and Str. thermophilus while the lowest inhibition was shown by L. delubricii. Results showed also that the temperature optimal for the growth of bacterial species (except L. casei and Str. thermophilus) tested and their inhibition activity was 30 °C. However, temperature of 35 °C was the optimal for the highest inhibitory effect of both L. casei and Str. thermophilus. At 25 °C of studied bacterial species showed decreased inhibitory effect against the tested fungi. Concerning the pH, the present study showed that the highest inhibitory of all tested bacteria species (except L. acidophilus) was at pH 6.5, L. acidophilus showed such activity at pH 5.5. This study revealed that the inhibitory effect of metabolites produced mixture of the tested bacterial species was directly proportional with its concentrate. Where this mixture was used at media concentration of 10, 20 and 40 mg/ml cultural. However, metabolic products from L. acidophilus, Leu. mesentroides and Str. thermophilus was the most effective in inhibition activity compared with the rest of the bacteria species used particularly at 40 mg/ml cultural media
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2010
الموضوع العام: علوم الحياة
اسم المشرف: كركز محمد ثلج الجبوري
اسم المؤلف: لؤي برهان مصطفى محمد

دراسة بكتريولوجية لبيئة بعض كهوف شمال العراق والتحري عن الدور الجرثومي فيها A Bacteriological Study on Some North Iraqi Caves Environment And Detection on The Bacterial Role in It

المستخلص: تضمنت الدراسة اجراء عدد من الفحوصات البيئية والبكتريولوجية على عينات الصخور ومياه النضح البالغ عددها 100 عينة والماخوذة من بعض كهوف المنطقة الشمالية في العراق التي شملت بهيري, شرانش, خنس, بجيل وانشكي للفترة ما بين تشرين الثاني - 2010 لغاية نيسان - 2011.ت | This study was included some of ecological and bacteriological tests on rocks and dripping water samples which were included 100 samples taken from some of north region caves in Iraq including Beherii, Sharanish, Khanas, Bejeel and Inishky from the period between November - 2010 to April - 2011.The results of ecological tests which represented with cave environment temperature were ranged from (13 - 24)?C and dripping water temperature were ranged from (11 - 20)?C while the results of pH values for the same sample shows simple variation between (7 - 8) for dripping water and (6 - 9) for the rocks samples. The results of geological screening and mineralization by using X - Ray Fluorescence and X - Ray - Diffraction showed that Beherii cave was mainly composed of manganese with 102 ppm comparing with other caves. While the concentration of iron and sulfur oxides were very low for all caves. The rocks of Beherii and Khanas caves were mainly composed of Dolomite (CaMg (CO3)2), Sharanish cave was composed of Calcite (CaCO3) and Dolomite with the closed ratio, while the rocks of Bejeel and Inishky were composed mainly of Calcite. The isolated bacteria in this study were identified according to cultural and morphological characteristics and biochemical tests, while the bacillus species were identified by using Microgen Bacillus Id System. Twenty two bacterial isolates were identified from total samples. There were nine (9)identified isolates from Beherii cave with 40.9% including Arthrobacter spp., Lysobacter spp., Caulobacter spp., Bacillus freudenreichii and Bacillus subtilis in with 4.5% for each isolate, while there was 18.1% of Methanogenic bacteria with its four genuses including Methanobacterium spp., Methanobrevibacter spp. Methanospirillium spp. and Methanosata spp. In Sharanish cave there was one isolate of sulfur oxidizing bacteria with 4.5 % from the total isolates. Three isolates were identified from Khanas cave included Pseudomonas spp., Xanthobacter spp., Clostridium spp. with 4.5% for each isolate. In addition, it was obtained from a cave Bejeel on six isolates included Bacillus lichiniformis, Bacillus lentus and 2 isolates of Bacillus cereus group, Streptomyces spp. and Gallionella spp. with 4.5% for each isolate and Merismopedia spp., Myxococcus Xanthus, Clostridium spp. were Isolated from Inishky cave with 4.5%for each isolate. The relation between some of bacterial species were studied by detection of the lytic ability of Lysobacter spp. and Myxococcus xanthus for Arthrobacter spp.The microbial role in caves were studied by detection of the ability of some isolated bacteria in Calcium Carbonate (calcite) formation and estimatation of calcite production, Xanthobacter spp. was the highest productive bacteria which gives0.2073 gram during 14 days comparing with other bacterial species.In addition, the effect of temperature and pH on calcite formation by Bacillus freudenreichii were studied, the results showed variation on calcite shape in 20?C and 30?C while the highest value of calcite production in pH.8 was 0.1336 gram comparing with pH.9 with 0.0190 calcite gram. The study also detected the ability of some isolated bacteria to oxidize of iron, manganese and sulfur, where Streptomyces spp. Gallionella spp. and one isolate of bacillus cereus group have shown the ability of oxidize iron on leptothrix medium while Bacillus freudenreichii, Bacillus lichinformis, Bacillus lentus, one isolate of Bacillus cereus group and Streptomyces spp. have shown the ability to the oxidize of manganese on Krumbein and Altmann Agar Medium. In addition, sulfur oxidizing bacteria has shown ability to oxidize sulfur on M.S. Agar and produce sulfuric acid as a product of the oxidation process.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: جهاد ذياب محل الجنابي اميرة محمود محمد الراوي
اسم المؤلف: امنة غانم عمر العاني

دراسة انتاج صبغة البرودجيوسين من بكتريا Serratia marcescens المعزولة من مصادر سريرية وبيئية وتاثيرها في بعض الميكروبات المرضية Study of the Prodigiosin productivity from Serratia marcescens isolated from environmental and clinical sources and the effect on some pathogenic microbes

المستخلص: The cotemporary world witnesses currently a rapid change due to the challenges imposed by the information technology in all scientific aspects.Hence, the scientific knowledge has become a prerequisite for each individual in this world. This led to a new view on education, in that it is no longer to be a process of providing information but it is a technique for a better learning.However, the main goal of education has shifted to focus on how to make the student to think appropriately and how to utilize the skills of the scientific thinking in order to motivate their thinking and understanding of knowledge as well as discovering the facts and relationships concerning the scientific phenomena aiming to form the generalities. The problem of the current research is mainly concerned with specifying the classical methods adopted in the Iraqi schools which do not match the contemporary scientific development. This research aims at utilizing the strategy of the strategy of forming generalizations according to Helda Taba Inductive Model as a teaching technique in order to identify its effect on : 1 - achievement in Biology.2 - the skills of the scientific thinking of the Fifth - Year female pupils.For sake of achieving the above two aims, two null hypotheses have been formed as follows : 1 - There are no statistically significant differences at level of significance of 0.05 between the mean scres of the female pupils (the experimental gronp), who have been taught according to the strategy of forming generalizations the strategy of forming generalizations, and the mean scores of those female pupils who have been twght classically in the achievnent test. 2 - there are no statistically siguificant differences at level of significance between the experiemental group, who have been taught according to the strategy of forming generalizations, and the mean scores of those female pupils who have been taught classically in the test of the scienhific thinting skills. In order to verify the above two hypotheses, the researcher has conducted an experiment which lasted for one whole academic semester.This experiment inluded : - The lesson plans. - The scientific thinking skills test (30 items). - An achievement test (60 items).The discrimination and difficulty power as well as the alternative action of the two test have been computed.Moreover, validity and reliability of the two tests have been secured. The reliability of the scientific thinking test has been found out to be (0.75) and the reliability of the achievcment test has been found out to be (0.92). This was done by using koder Richardson formula - 20. The researcher has prepared an experimental design, comprising two groups, the first one of which is called the experimental group which is to be taught according to the strategy of forming generalizations.While the second group is called the control one which is to be taught classically. The sample of the study covers (46) female pupils at the fifth - year secondany school (scienbific branch).The whole sample have been randomly distributed into two groups, the experimental one is of (24)pupils and the control one is of (22)pupils. The equalization between the testees in the variables of age, intelligence, exinformation, previous achivement in Biology and the scientific thinking skills has been conducted. The experiment group was conducted at the second academic semester (7weeks).The researcher himself had taught the two groups.The results obtained has been statistically analyzed by using two - indepenbent samples t - test.The results gained showed a better performance of the experimental group more than that of the control one in the scientific thinking and the achievement tests. In the light of the above results, the researcher reconmends the use of the strategy of forming generalizations in accordance to Helda Taba Inductive Model.Also, the researcher recommends to conduct other studies and research on other subjccts other than Biology on different school stages to find out the impact of the strategy of forming generalizations on thinking skills.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: مكرم ضياء جعفر شكارة صالح عبد الرضا الصالح البكري
اسم المؤلف: فرزدق ناظم حربي البديري

دراسة تاثير مياه مجاري سامراء على نوعية المياه في نهر دجلة A Study on The Effect of Samarra Sewage Water on The Quality of Water in Tigris River

المستخلص: تضمنت هذه الدراسة اختيار خمس محطات اربعة منها على نهر دجلة المار بمدينة سامراء بعد جسر سامراء والمحطة الخامسة والتي تمثل رقم 2 على مياه مجاري سامراء قبل ان تصب في مياه نهر دجلة.وقد تم اخذ العينات للمدة من شهر تشرين الاول 2010 حتى نهاية شهر ايلول 2011 وقد | The study included chosen five stations for water sampleing, four of them at Tigris river while the fifth station was chosen at samara sewage before meeting with Tigris river.Samples were collected monthly started from October 2010 until the end of September 2011. The study included the study of some physical, chemical and biological characteristics for river water and sewage and noticed the effects of sewage water on quality of these water in Tigris River, these characteristics clued in air , water temperature, electrical conductivity, pH, total dissolved solid, Total Suspseneded solid, dissolved oxygen(Do), biological oxygen demand (BOD), total hardness, calcium and magnesium hardness, chloride, alkalinity, nutrients concerteration, ( phosphate, nitrate) in addition the total number of bacteria and coliform bacteria.Water Temperature ranged through out the study ranged between (5° - 35°), while air temperature ranged between(5° - 38°) , Electrical Conductivity in the river ranged between (280 - 1480) mc/cm, where in sewage the electrical conductivity ranged between(1200 - 2180) mc/cm, while pH value tened to be slightly alkaline, it show very close value where ranged between(5.3 - 8.8). It was noticed that the pH value tened to be slightly acidic from January to August 2011. The results also show that the water were in good airation where the value of dissolved oxygen ranged between (3 - 10) mg/L in station one and stations five while the value of dissolved oxygen in stations two and three range between (2 - 6.5) mg/L.The result also showed that the concentration of total suspended solids ranged between(20 - 798) mg/L. where it has high value in April in all stations, this propably due to increase of discharge of the river which accompanied by suspended solid, while the dissolved solids in all station range between (250 - 1470) mg/L.The highest value recorded were, 1470 mg/ L on May 2011in station two.The value of total hardness for Tigris river ranged between hard to very hard(125 - 360) mgCaCO3/L in station one, four and five, whereas its value in stations two and three ranged between (200 - 925) mg/L. Calcium ion which is the cause of calcium hardness was higher than magnesium cencenrteration which is responsible of magnesium hardness, where calcium hardness ranged between (75 - 587.5) mg/ l and magnesium hardness ranged between (5 - 500) mg/l. chloride concentration ranged between (8 - 127.5) mg/l in all station, chloride cencetration in stations one, two, and five were in the allowable wnorld limit(8.5 - 68) mg/l. Alkalinity results showed that it related to carbonate and bicarbonate, and its ranged between (110 - 900) mg/l.While phosphate value ranged between(0.0106 - 1.999) µg atom - PO4/L, the lowest values were recorded in Tigris river, While the high value recorded in sewage station two, while nitrate value were fluctuated in all stations but the high value recorded in the second station is ranged between (0.0103 - 1.140) µg atom NO3/l. Result of microbiological study shown that there is high number of bacteria the average of total count of bacteria were(39.7×105، 17.31×105، 267× 105، 3.17 ×105، 0.0531×105) cell/ ml for all stations respectively, so it has been suggested that the water in Tigris river considered as polluted to very polluted while the resulty of Coliform bacteria were very high and the average number for all stations respectively as follows (209 × 105, 9809×105 , 25× 105, 1×105, 0.004×105 ( cell /ml According to these result the water is not suitable for direct drinking.Statistical analysis for most factors studied show significant differences between station one and station two and between station one and station three as well in stations four and five and there is no significant differences between station two and station three.
الصفحات الاولى:
الكلمات الدلالية:
  • البيئة - التلوث البيئي
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: حميد سلمان خميس المهداوي ياسين حسين عويد
اسم المؤلف: افراح طعمة خلف مطر البدري

التحري عن طفيلي المقوسة الكوندية Toxoplasma gondii في الطيور الداجنة في منطقة الفرات الاوسط والقطط في محافظة الديوانية باستخدام التقنيات المصلية والجزيئية Detection of Toxoplasma Gondii In Domestic Avian In Middle Euphrates Region And Cats In Al - Diwania Province By Serological And Molecular Techniques

المستخلص: تضمنت الدراسة الحالية الكشف عن طفيلي المقوسة الكوندية Toxoplasma gondiiفي اربع انواع من الطيور ضمت الدجاج المحلي Gallus gallus domesticus والديك الروميMeleagri galbpavol والاوز الاربد Anser anser والبط المحلي Anas platyrhychos domesticus في منطقة الفرات ا | The current study was conducted to detect of Toxoplasma gondii in five species of avian including, Gallus gallus domesticus, Meleagris galbpavol, Anser anser, Anasplatyrhychos domesticus in middle Euphrates provinces of Iraq, also in cats in AL - Diwania province, during 2011 - 2013 by using serological tests (Latex, Rapid test cassette) and molecular test (conventional - PCR and Nested - PCR) as confirmative tests to detect of B1gene which was specific gene of T.gondii in blood and Tissue samples of different organs such as Liver, Heart, Brain, Eye, Lung, Pectoral muscles, Small intestine, Spleen, and Leg muscles of avian and cats which were seropositive cases of Rapid test cassette, also the study was included the detection Oocysts of T.gondii in soil samples of ten cities from Al - Diwania province by using sedimentation and floatation methods as primarily diagnosis, also using the polymerase chain reaction to detect of B1gene in these samples. A total of 320 Avian collected, include 80 samples of each species (20 samples of chicken, Turkey, geese, and Ducks) of each province of middle Euphrates (AL - Diwania, Babylon, AL - Najaf and Karbala), in addition to 10 samples of cats and 100 samples of soil from the center of AL - Diwania province, samples of avian and cats were involved to serological test such as LAT, the results showed there were 96 positive samples in percentage %30, include, 31(%38.75) of chicken, 28 (%35) of turkey, 22 (%27.5) of geese, 15 (%18.75) of ducks and 5 (%50) of cats, the higher Percentage (%23.95) of infection with T. gondii in avian was in titer 1/160 and the lower percentage (%3.73) in titer 1/640, Whereas in cats, the higher percentage in titer 1/20 and lower percentage in titers 1/40, 1/80, 1/160 which reaches %20. The results of Rapid test cassette were showed there were 60 out of 320 samples in percentage %18.75, include 24 (%30) of chicken, 19 in (%23.75) of turkey, 10(%12.5) of geese, 7(% 8.75) of duck and 3(%30) of cats. Also the results of Rapid test cassette were showed the higher percentage of types of antibodies in the serum of avian was %36.66 of IgG +IgM together and the lower percentage was %28.33 of IgM, whereas in cats, the higher percentage was %66.66 of IgG and the lower percentage was %33.34 of IgG +IgM together. To confirm the infection of avian by T.gondii, Polymerase Chain Reaction was used to detect the B1 gene (399 bp) in 320 blood samples of avian and 10 samples of cats, the results were showed there were 34 samples (%10.62) of avian gave positive result, include, 14 (%17.5) of chicken, 12 (%15) of Turkey, 8 (%10) of geese, whereas the results were showed the B1 gene was not present in the blood samples of ducks and cats. The results of Latex, Rapid test cassette and polymerase Chain Reaction were showed, the higher percentage was recorded in chicken % 17.5, %30, %35, whereas the lower percentages were %18.75, %8.75, %0 in D ucks by using the three tests, respectively. According to the results of Rapid test cassette (IgG, IgM +IgG), the results of polymerase Chain Reaction to detect the B1 gene in 16 samples of avian (4 samples of each species) out of 39 samples and 3 samples of cats, showed there were 9 samples (%56.25), include 4 (%100) of chickens, 3(%75) of turkey, 1(%25) of geese, and 2(%66.66) of cats and the higher percentage of B1gene (399bp) in livers of avian was %55.55, %22.22 in pectoral muscles then heart, spleen and small intestine in percentage %11.11 for each one of them, whereas in cats the percentage were % 66.66 in liver while in brain and kidney the percentage was %33.34 of each one of them.. The results of Nested Polymerase Chain Reaction for amplification the gene B1 in 12 blood samples (4 samples of each species of birds except ducks) was positive for conventional - PCR, Showed the amplification occurs in 4 samples in percentage % 33.33 during the first round of reaction and 3 samples in percentage %75 in the second round, in 10 samples of tissues, the amplification occurred in 6 samples in percentage %60 during the first round and in 4 samples in percentage % 66.66 during the second round of reaction. Also the results were showed the percentage of T.gondii antibodies in serum of four types of avian (except pigeons) according to the provinces were %28.75 in AL - Diwania, %40 (Higher percentage) in Babylon, %12.5(Low percentage) in AL - Najaf and %38.75 in Karbala by latex test, whereas the percentages %26.25 (higher percentage), %10 (lower percentage), %12.25, %17.5 in each of Karbala, AL - Najaf, Babylon, AL - Diwania province respectively by Rapid test cassette, and by using polymerase chain reaction, the percentages were %16. 25 (Higher percentage), %5(Lower percentage), %6.25, %15 in AL - Diwania, Babylon, AL - Najaf and Karbala province, respectively. Also the results showed, the percentage of infection in AL - Diwania province the samples of rural areas were higher (%37.5, %22.5, %17.5) whereas the percentages were lower (%20, %12.5, %12.5) in urban areas by using latex, rapid test cassette, and polymerase chain reaction, respectively. The results of comparison between two serological tests and Polymerase Chain Reaction, showed the percentages of infection were % 10.62 (34), %18.75 (60), %30 (96) by using Polymerase chain reaction, Rapid test cassette, latex test, respectively, also the sensitivity and specificity of latex test were %35, %72 whereas the sensitivity and specificity of Rapid test cassette were %35, %88, respectively in compared with standard Polymerase Chain Reaction. In the soil samples, the percentage of presence of Oocysts of T.gondii was %10 by using floatation method and %4 by conventional Polymerase Chain Reaction. The study of grossly and microscopy pathological changes in avian and cats which infected with T. gondii, was showed there were lesions on each of liver, heart, pectoral muscles, small intestine, brain and kidney.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دكتوراه
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: خيري عبد الله داود العكيلي هادي مدلول الميالي
اسم المؤلف: خديجة عبيس حمود الخالدي

عوامل الخطورة لطفرات الجينين CBS وMTHFR لقبل الارتعاج لدى النساء الحوامل في النجف Risk Factors of CBS And MTHFR Gene Mutation For Preeclampsia In Pregnant Women In Al - Najaf

المستخلص: تعد الاصابة بقبل الارتعاجPre - eclampsia من الاعتلالات الخطيرة التي قد تظهر في الثلث الثالث من مدة الحمل اذ تتصف هذه الاصابة بارتفاع ضغط الدم وظهور بروتين الالبومين في الادرار. وقد ظلت اسباب نشوء هذه الحالة غير واضحة المعالم, اذ اعزيت بعض من اسبابها الى ا | Preeclampsia is considered as one of the serious complication in the third trimester of pregnancy. It is associated with hypertension and proteinuria. The causes of preeclamptic are still not well understood. Evidences suggested the involvement of metabolic related mutations in the disease. In the current study, the genetic mutations of CBS and MTHFR genes are studied as markers for the diagnosis of preeclampsia. Sixty preeclamptic pregnant women and forty healthy pregnant women as control group diagnosed by the physicians at Azzahra Teaching hospital for obstetrics &pediatrics in Najaf province from December 2012 to August 2013 The poly merase chain reactions technique was used to evaluate the incidence and frequency of C?S &MTHFR mutations. A questionnaire was conducted to obtain the information of the patients and the control group. Genetic mutation were detected by polymerase chain reaction (PCR)and agaros gel electrophoresis. The information indicated that the age of pregnant women, gestation age, previous history of the patients, family history and parity are involved in the pathogenesis of preeclampsia. The results of the present study of preeclampsia showed a significant increase (P ? 0.01) in patients (25 - 34) years old when compared with the other group. where the results found a significant increase (P <0.01) of Preeclampsia occurred during (33 - 39) weeks of pregnancy. And in the patients with previous history for preeclampsia there was significant increase (P<0.001) compared with healthy pregnants. Pregnants with preeclampsia in the family history showed significant increase (P< 0.01) as for women pregnant for the first time showed and significant (P<0.01) and constituted 8% pregnanst with preeclampsia who had diabetes mellitus. The extraction and quantification of cell free fetal DNA (cff DNA) revealed a significant (P<0.0001)increase in preeclamptic patients when compared with those of healthy pregnant women. It is confirmed by the use of an internal negative control through the monitoring of SRY gene (Y chromosome gene) amplification that the gene belong to the infant and 40 pregnant woman were followed up till the labor. Thus, 9 of them had delivered males, while 30 had delivered females. The wild type SRY gene band was obtained clearly from those of male delivery, but not from those of female delivery. Mutations of C?S gene were analyzed by theuse a primer intended to amplify a fragment of171 bp.The rate of mutation was found to be 51.7% and 5% in preeclamptic patients and healthy pregnant women respectively and Odd Ratio=20.31, P value=10 - 7. The C677T mutation of MTHFR gene was verified by the use of a primer designed to amplify a fragment of 198bp. The prevalence of mutation was indicated to be 68.3% and 17.5% in the preeclamptic patients and healthy pregnant women respectively and Odd Ratio =10.17, Pvalue =6.2*10 - 7. Results demonstrated the wide involvement of C?S and MTHFR gene mutation in the pathogenesis of preeclampsia, but they are no crucial for the development of the disease. In addition there are so many factors thatmay be involved in such development.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ظافرة جعفر عبد علي الفتلاوي ماجد كاظم حسين
اسم المؤلف: زهراء سامي رزاق النجار

تاثير كبريتات الخارصين في احداث افات نسجية مرضية وتغيرات وزنية في بعض اعضاء الفئران البيض Mus musculuc الحوامل وتشوهات خلقية ظاهرية لاجنتها Effect of Zinc Sulfate in Histopathological Lesions Production And Weight Changes in Pregnant White Mice Mus Musculus And Morphological Congenital Malformations of It's Embryos

المستخلص: درس قابلية الجرعات العالية من كبريتات الخارصين في احداث تغيرات مرضية نسجية في اناث الفار الابيض السويسري الحوامل وتشوهات خلقية في اجنتها، حيث جرعت الحوامل بمحلول كبريتات الخارصين المائية داخل المرئ، حيث قسمت الاناث الى خمس مجاميع الاولى : جرعت بماء مقطر
الصفحات الاولى:
الكلمات الدلالية:
  • الحيوان - الانسجة
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: عزيز خالد حميد سهام توفيق امين
اسم المؤلف: محمود نوفل مصطفى حمد الصالح

دراسة بعض الجوانب المناعية لاصابات اللشمانيا في عينة المرضى العراقيين Study of Some Immunological Aspects of Leishmaniasis In Sample of Iraqi Patient

المستخلص: This study was carried on 145 patients infected with leishmaniasis including; 75 with CL, 70 with VL, attending Al - Karama Teaching Hospital, Central Public Health Laboratory and Central Pediatric Teaching Hospital, during the period October 2013 to February 2014 were enrolled in this study, in addition to 30 healthy control collected from the National Center for blood donation for the same age groups for a group of patients. No clinical autoimmune manifestations such as arthralgia or cutaneous vasculitis were reported in any patients. Based on direct skin smear examination and serum samples analysis, the patients were distributed into two clinical groups : 30 patients suffering from cutaneous leishmaniasis and 30 patients suffering from visceral leishmaniasis patients.The presented study aimed to confirmation of diagnosis cutaneous leishmaniasis depending on direct skin smear and visceral leishmaniasis based on indirect immunofluorescent assay test IFAT, then investigate the association of IgG autoantibodies against some antigens, especially : nuclear antigens, like : ds - DNA, nucleosome, histones, SmD1, PCNA, ribosomal P0, SS - A/Ro60kD, SS - A/Ro52kD, SS - B/La, CENP - B, Scl70, U1 - snRNP, AMA M2, Jo1, PM - Scl, Mi - 2, Ku and against cardiolipin, in order to correlate the types of diseases presentation (cutaneous or visceral) with the presence of these autoantibodies in a sample of Iraqi patients.The study was reached to the following results : 1. The direct microscopically examination of skin smear in CL patients, showed positive results in 30 cases (40.0%) compared to 45 cases (60.0%) negative results, and was a significant difference at the level of (P?0.05).2. The anti - leishmanial antibodies by Indirect immunofluorescent assay test in VL patients, showed positive results in 30 cases (42.9%) compared to 45 cases (57.1%) negative results, and was a highly significant difference at the level of (P? 0.01).3. The age groups most affected in CL were 16 to 40 years, with high significant differences at the level of (P? 0.01) were observed when compared with the other age groups. As for patients with VL, who aged between 3 months to 9 years, they showed variable significant differences between age groups in female while there were no significant differences in male at the level of (P?0.01). The majority of patients were aged 3 to 4 years and they represent 9 (30.0%). The study result showed 16 CL patients (53.3%) have only one lesion, while 11 patients (36.7%) have 2 to 9 lesions and only 3 cases (10%) have ? 10 lesions in different sites of their bodies. There were elevated high significant differences at the level of (P?0.01) when compared between the groups. And 21 cases (70%) have infected for ? two months, and the remaining 9 cases get their infection for > 2 months duration. There were increased high significant differences observed at the level of (P?0.01) when compared between the groups. Dry lesions recorded superiority in 20 (66.7%) of the CL cases, and other 10 (33.3%) have been diagnosed as a wet lesions. There were elevated high significant differences observed at the level of (P?0.01) when compared between the two types of the lesion. And The upper limbs were the dominant site of lesions in the CL cases, 15 (39.4%) of the cases had their lesions in one or both hands, followed by the lower limbs in 11 (29.0%) cases and the less frequent site of infection was the face in only 4 (10.5%) cases and 8 (21.0%) cases common lesions. The results showed obvious high significant differences at (P? 0.01) between the groups.4. For the tests of the autoantibodies, the anti - ds - DNA antibody was positive only in one patients (3.3%) with CL of the total (30) patients, while tests of the rest 29 (96.7%) were negative, while in VL of the total (30) patients, 9 patients (30.3%) were positive and 21 (70.0%) were negative. The anti - ds - DNA in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the study groups. As for the anti - nucleosomes antibodies, it was positive in 5 patients (16.7%) and negative in 25 (83.3%) among patients in CL, while in VL patients, 16 patients (% 53.3) registered positive and 14 (46.7%) their result werenegative, the anti - nucleosomes antibodies in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the groups study. No score was recorded for anti - SmD1antibodies in CL patients, no one was positive 0 (0.0%) and all the 30 patients (100.0%) gave negative result for this antibody, while in patients with VL, the result showed 4 patients (13.3%) gave positive and 26 patients (86.7%) were negative, the anti - SmD1antibodies in leishmaniasis patients was significantly different at the level of (P?0.05) when compared between the groups. While the anti - SSA/Ro60kD and anti - SSA/Ro52kD, in VL patients, 9 patients (30.0%) scored positive results and 21 of the patients (70.0%) were tested negative for anti - SS - A/Ro60kD, but 7 patients (23.3%) were positive and 23 of the patients (76.7%) were tested negative for anti - SS - A/Ro52kD. While in patients with CL, no one scored positive (0.00%) and 30 of the patients (100.0%) were negative for anti - SS - A/Ro60kD, whereas one patient (3.3%) gave a positive result and 29 of the patients (96.7%) were negative for anti - SS - A/Ro52kD. About anti - SS - B/La we found that 19 of the patients (63.3%) were scored positive result for this antibody in addition to 11 patients (36.7%) who were negative in VL patients and 4 of the patients (13.3%) have those antibodies and 26 of the patients (86.7%) haven’t these antibodies in CL patients respectively. The anti - SS - A/Ro60kD, anti - SS - A/Ro52kD and anti - SS - B/La in leishmaniasis patients were highly significantly different at the level of (P?0.01), when compared between studied groups.5. VL cases showed a significantly different distribution of anti - nuclear antibodies (ANA) in compare with CL cases, especially; in Anti - Ku antibodies 24 (80%), anti - Mi - 2 antibodies 21 (70%) and anti - SS - B/La antibodies 19 (63.3%) respectively.6. The results of anti - nuclear antibodies level in patients with leishmaniasis like; anti - ds DNA, anti - nucleosome, anti - SmD1, anti - PCNA, anti - SS - A/RO 60, anti - SS - A/RO 52, anti - CNP - B were in high significantly different at the level of (P?0.01) and level (P?0.05) when compared between the groups, while the other autoantibodies like; anti - histone, anti - ribosomal P0, anti - Sc170, anti - U1 - snRNP, anti - AMA M2, anti - J0 - 1 and anti - PM - Sc1 showed no significant differences (P?0.01) between the investigated groups.7. The mean value of anti - cardiolipin IgG antibodies was increased substantially with highly significantly differences (P?0.01) in VL patients as compared with control group (8.123 vs. 1.959), also as compared with CL patients (8.123 vs. 2.402).
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: انوار ادريس سليمان العساف
اسم المؤلف: مرتضى قيس علي العامري

دراسة تاثير جنس الجنين وبعض المتغيرات الفسلجية في تراكيز بعض الهرمونات والمتغيرات الاخرى في دم الحوامل لمدينة تكريت وضواحيها Study The Effect of Fetus Gender And Some Physiological Variables on The Concentrations of Certain Hormones And Other Variables on Pregnant Blood in Tikrit City And Its Suburbs

المستخلص: جرت هذه الدراسة في مختبرات مستشفى تكريت التعليمي ومراكز الرعاية الصحية ومختبر علوم الحياة في كلية التربية للبنات جامعة تكريت وضواحيها للفترة من تشرين الاول2011 ولغاية اذار 2012 لمعرفة تاثير مراحل الحمل وعوامل جنس الجنين وكتلة الجسم الحي وعمر الام والسكن ل | This study was conducted in the laboratories of Tikrit Teaching Hospital, health - care centers, and the laboratory of Biology in college girls education in Tikrit city and its suburbs from the period of October 2011 until March 2012. The study was performed to see the impact of the pregnancy stages; sex factors of the fetus; body mass index; the mother's age; and residence for pregnant women, in the concentration of : certain blood components (Hb, PCV, WBC); blood sugar; cholesterol; estrogen, progesterone and testosterone hormones; and (TSH, T3, and T4 hormones. The study included (90) women at different stages of pregnancy, and the number of pregnant women at every stage was (30), ranged their ages from (15 - 40) years. A sample of (10) ml blood was taken from the surveyed women. The serum was separated by the common methods of separation, and conducted thereon desired examinations, The outcomes were as follows : - 1. Superiority of the first and second stages of pregnancy significantly (P<0.05) in the concentration of (Hb, PCV), compared with the third stage. 2. Increasing the total count of white blood cells and the concentration of blood sugar and cholesterol significantly with the progress of pregnancy stages. 3. Increasing progesterone hormone concentration significantly with the progress of pregnancy stages, while the concentration of estrogen hormone decreased in the middle stage of pregnancy, and increased significantly in the first and third stages, whereas there were no significant differences in the concentrations of testosterone hormone during the different stages of pregnancy. 4. The concentration of TSH and T3 hormones were decreased significantly with the progress of pregnancy stages, while the concentration of T4 hormone was increased in the first and last stages of pregnancy.5. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of Hb and PCV ratios during the first and second stages of pregnancy, while the opposite happened in the third stage.6. Superiority of male pregnancy significantly compared with the female pregnancy in the total count of white blood cells during the first and third stages of pregnancy.7. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of blood sugar, while the female pregnancy surpassed significantly in the concentrations of cholesterol compared with the male pregnancy during all the pregnancy stages. 8. Superiority of female pregnancy significantly compared with the male pregnancy in the concentration of estrogen hormone in first stage, while the opposite happened in the third stage, while the male pregnancy surpassed significantly compared with the female pregnancy in the concentrations of testosterone hormone during second and third stages, as well as superiority of female pregnancy significantly compared with the male pregnancy in the concentration of progesterone hormone during the first and third stages of pregnancy, while the opposite happened in the second stage.9. Superiority of female pregnancy significantly compared with the male pregnancy in the concentrations of TSH hormone during the first stage of pregnancy, while the opposite happened in the third stage. while Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of T4 hormones during the first and second stage of pregnancy but the concentration of T3 superiority all pregnancy stages.10. A significant decrease in the values of Hb and PCV with increasing the body mass index during the second stage of pregnancy while the opposite happened in third stage of pregnancy.11. Increasing the total count of white blood cells significantly with increasing the body mass index in the first stage of pregnancy, while the opposite happened in the last stage of pregnancy. Increasing the concentration of blood sugar with increasing body mass index in first and second stages of pregnancy but concentration cholesterol decreasing significantly with increasing the body mass index.12. Decrease the concentrations of estrogen hormone significantly during first and second stages of pregnancy with increasing the body mass index for pregnant women, while a significant increase appeared in the concentration of progesterone hormone with increasing the body mass index during the second stage of pregnancy, and the opposite happened during the first and third stages. Testosterone hormone increased significantly with increasing the body mass index for pregnant women during first and second stages of pregnancy 13. A significant increase appeared in the concentrations of TSH hormone at pregnant women with decreasing the body mass index. while a significant increase for the concentration of T3 in first stage and T4 hormone increase at pregnant women during all stages of pregnancy with increasing the values of body mass index.14. No significant differences in values of Hb and PCV during the first and second stages of pregnancy for all ages, but the small ages surpassed significantly compared with the old ages during the third stage of pregnancy. while The total count of white blood cells decreased significantly with the progress of pregnant women's age during all pregnancy stages.15. The small age groups for pregnant women increased significantly in the concentration of blood sugar during the first stage of pregnancy, while they decreased significantly during the second stage of pregnancy.while Age did not affect significantly in the concentrations of cholesterol during the first stage of pregnancy, while the old age groups decreased significantly compared with the first and second age groups) 32 - 24), (23 - 15) during the second and third stages of pregnancy.16. The concentration of Estrogen hormone increased significantly with the age progress during the first and third stages of pregnancy compared with the second stage, while the concentrations of progesterone hormone decreased significantly at old ages during the first and third stages of pregnancy.The concentration of testosterone hormone was not affected significantly during the last stage of pregnancy for all ages, but it decreased significantly during the second stage of pregnancy compared with the first stage of pregnancy for all ages.17. A significant increase showed in for the concentration of TSH in first and the last stages while decreased significantly the concentration T3 and T4 hormones with the age - progress at all stages of age.18. No noteworthy impact for residence of pregnant women in all stages of age, cause its impact is very little on all the qualities covered in the present study.
الصفحات الاولى:
الكلمات الدلالية:
  • الحيوان - الفسلجة
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: وهبي عبد القادر سلمان
اسم المؤلف: مروة عصام سليمان

تاثير بعضز الظروف البيئية على النظام الدفاعي المضاد للتاكسد خارج الخلوي لدى المدخنين والعاملين في مجال اللحام والاشعاع Effect of Some Environmental Conditions On The Extra - Cellular Defense System Among Smokers And Workers In The Field of Radiation And Welding

المستخلص: تعد ظاهرة الاجهاد التاكسدي من اهم الميكانيكيات المحتملة لاحداث الضرر في النظام الحيوي عند التعرض لانواع مختلفة من المؤثرات والعوامل البيئية، ولذا تكون الانظمة المضادة للتاكسد هي اول من يتاثر بالزيادة الحاصلة في تكوين الجذور الفعالة المؤكسدة. لذلك تهدف هذ | The phenomenon of excessive oxidation considered the most important mechanism that cause potential damage to vital system when exposed to different forms of environmental factors, so anti - oxidants systems are the first to be affected by the increase formation of oxidizing radicals. For this reason, This study aims to determine the extent of the effect of some environmental conditions on the Extra - cellular antioxidant system, determine the most affected part in this system, study the correlation of this damage with other variable and to identify the natural values of different antioxidant system components. The study was carried out in the district of Baquba city, capital of Diyala province during the period from 10 October 2013 to 1 May 2014, the study group included (160) individual divided into four group (40) persons working in the field of welding, (40) persons workers in the radiation and (40) persons smokers and compared with (40) healthy people (the control group), all individual included in this study were male with age rang (25 - 45 years).Requested blood samples were taken for laboratory testes including complete blood count, and measurement of the following biochemical parameters, total serum protein, serum albumin, serum zinc, serum copper, serum iron, serum glutathione and malondialdehyde. The results of the laboratory test elicited that, the most common abnormalities in this study were the presence of low level of glutathione zinc, and copper in individual in all three study group in compare with controlled group and the differences were statistically significant(p value<0.05, <0.001, <0.01 respectively), also low level of iron in radiation group in compare with controlled groups and the differences were statistically significant(p value<0.01), and low level of proteins and albumin in smoking group in compare with controlled group and the differences were statistically significant(p value<0.01).also results of the current study revealed increased in the level malondialdehyde (MDA) in all three study groups in compare with controlled group and the differences were statically significant (p value<0.01). and increased levels of protein and albumin in the radiation and welder group in compare with controlled groups and the differences were statistically significant(p value<0.05) and increased in the level of iron in welder and smokers compare with control group and the differences were statistically significant (p value<0.001). The results of other laboratory test which include the complete blood count show High levels of white blood cells in all study groups compare with control group and the differences were statically significant (p value<0.001), and High levels of neutrophil and lymphocyte in welder and radiation group compare with control group and the differences were statically significant (p value<0.001). Results of the current study also showed increased levels of hematocrit, and red blood cells count and platelets count in a smokers group compare with control group and the differences were statically significant (p value<0.01).also there is increased in red blood cells count in radiation group in compare with controlled group (p value<0.05) , and decreased in platelets count in radiation group in compared with controlled group (p value<0.05).
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - الفسلجة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: مازن رزوقي محمد
اسم المؤلف: معد رشيد مطلك الزبيدي

تاثير المعاملة ببعض مبيدات الافات والمواد الكيمياوية في نمو الفطرين Alternaria tenuissima وFusarium oxysporum المرافقة لبذور وجذور الحنطة Triticum aestiumn Effect of Some Pesticides And Chemicals In The Growth of Fungi Alternaria Tenuissima And Fusarium Oxysporum Associated With The Seeds And Roots of Wheat (Triticum Aestiumn)

المستخلص: اذ تم في هذه الدراسة عزل عدة انواع من الفطريات المرافقة لجذور وبذور الحنطة تموز 2 مصدق 1وتم تشخيص تسعة انواع من الفطريات المرافقة للبذور وبنسب تردد مختلفة وهذه الانواع هي Aspergillus niger, Penicillium notatum , Alternaria tenuissima, Rhizopus stotanifer | As it was in this study to isolate several types of fungi associated with the roots and seeds of wheat July 2 certified 1 was diagnosed nine types of fungi associated with seeds and different frequency rates and these types areAspergillus niger, Penicillium notatum, Alternaria tenuissima, Rhizopus stotanifer P. digitatum, Fusarium oxysporum, A.flavus, Fusarium solani, A. alternata. Was diagnosed nine types of fungi isolated from the roots of wheat and the surrounding soil and different frequency rates and these types. A. alternata, A. niger, R. stolanifer, Mucor sp., Trichoderma hazianum, P. notatum, F. oxysporum, F. solani, Alternaria tenuissima. Testing was diagnosed two fungal species tested using a technique monomer chain reaction PCR. These species are fungal Fusarium oxysporum, Alternaria tenuissima. This study tested the effect of pesticides is a fungal pesticide jungles Granstar and insecticide Wide. The transactions chemical which urea and NaCl in the growth of some fungi isolated from the seeds and roots of wheat as it has been test the effect of different treatments and their interaction in the germination of seeds of wheat on the filter paper and compare nets comparison innate Elsa (Carbendazim) and underwent tests the effects of different treatments and their interaction in radial growth and dry weight of the two types of fungi isolated in the germination of their spores and bacterial length of the tube, as well as test the effect of transactions and their interaction in the germination of seeds in the soil sterile and non - sterile. The results showed that the use of urea and sodium chloride concentration of 15 mg / mL in the treatment of seeds of wheat led to increased germination of these seeds and the impact of a negative in some types of pathogenic fungi associated with them as well as the use of pesticides other than fungal pesticide jungles Granstar and insecticide Wide negative effect also in the vitality Activity and fungi.germination The results showed the existence of significant effects on rates of seeds treated with pesticides and salts on the filter paper analogy transactions comparison since given concentration 15 mg / 10 mL of pesticides and 15 mg / ml of salt are high in the germination of the seed treatment, which exceeded ratios germination of seeds of wheat treatment urea fertilizer treatment fungicide Elsa. As rate ratios germination of wheat seed treatment with urea 96.66%, while the rate ratios germination of seeds of wheat pesticide Elsa 93.33%, followed by treatment of pesticide Wide as rate ratios germination 83.33% and 80.00% for wheat seed when the focus 15 mg / ml of sodium chloride salt treatment, and after treatment Karan Star as the rate of germination rates of seeds of wheat 73.33%. The results showed the existence of significant effects in increasing the proportion of seed germination treatment with pesticides and salts in the soil sterile and non - sterile as it gave the Focus 15 mg / 10 mL of pesticides and 15 mg / ml of salts highest rates of germination of these seeds came these results are compatible with the results of seed germination in the filter paper. The results show that as well as the individual transactions were more influential than the transactions overlap in radial growth and dry weight of fungi testers and their spores germination rates and lengths of germ tubes, as growth rates of radiographic Fungi F. oxysporum and A. tenuissima in the treatment of a mixture Wide and Granstar 11.44, 8.55 mm, respectively, while the rates of diameters of the colonies for the treatment of urea pesticide Wide 33.66 and 26.66 mm, while the pesticide mixture Wide treatment with sodium chloride amounted to 37.00 and 15.00 mm, in while rates were diameters of colonies for the treatment of a mixture Granstar with sodium chloride, 52.66 and 25.00 mm, and the growth rates were radiography for the treatment of pesticide Granstar with urea 25.00 and 11.66 mm, and the growth rates for the treatment of sodium chloride with urea 31.24 and 37.33 mm, compared with the fungicide Elsa, which amounted to the radial growth rates of 8.55 - 11.44 mm Fungi testers respectively.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عبد الامير سمير سعدون
اسم المؤلف: مهند جاسم حمود

تقويم صفة تحمل بعض اصناف البطاطا المكثرة نسيجيا للجفاف على المستوى الجزيئي Evaluation of Some Potato Varieties Grown In Vitro To Drought Tolerance At The Molecular Level

المستخلص: درست استجابة اربعة اصناف من البطاطا Solanum tuberosum L. وهي (Lusa, Ambo, Arizona, Riviera)، استجابتها للزراعة النسيجية، وتقييم صفة تحمل الجفاف، كما اجريت التحاليل الجزيئية لتحديد البصمة الوراثية لها.اوضحت نتائج تجربة اختبار استجابة الزراعة النسيجية، ان ا | Four varieties of Potato (Solanum tuberosum L. Lusa, Ambo, Arizona and Riviera) were studied for their response to tissue culture and evaluation of the treatment of drought tolerance and molecular analysis. Results showed that the varieties differ in their response to growth, where as Arizona was the best variety in shoot tip culture and the survival percent (100%) and gave the highest average of branch length and number of leaves which were 9.810 cm, 8.100 leaf/ plant, while Riviera showed lower rate of survival and growth (40%). Riviera was excluded from the experiments. Cytokines and auxins were used in the following concentrations Kinetin (kin) with 2, 4 mg/L and Benzel adenine (BA) with interacted with Indoleacetic acid (IAA) at 1 or 2 mg/l or Napthalene acetic acid (NAA) at 0.5 or 1.0 mg/L in multiplication experiment. Arizona variety exhibited the highest length shoot, number of nods, number of leaves, root length, dry weight of roots, fresh and dry weights of shoots when using kinetin 2mg/L; interacted with NAA at 0.5 mg/L. while it showed a significant increase in the average of branches number, stem height, fresh and dry weights of shoot as well as the number of roots after treatment with using BA at 1 mg/L in combination with 0.5 mg/L (IAA). Drought tolerance of the studied varieties was studied by using Poly Ethlene Glycol (PEG) at the concentrations 20, 40, 60, 70, or 80 g/l. It showed that proline content was at its highest in Ambo variety reached 22.811 ? /g at the concentration 80 gm/L PEG. Arizona gave the highest mean in number of branches, number of nods, number of leaves, number of roots, under water stress conditions when the medium was supplemental with 20 mg/L PEG. Molecular analysis the studied varieties was conducted by using 6 primers. Results showed that the inheritance approximation between them, 0.314, 0.297, 0.273 for Lusa, Ambo and Arizona respectively. These results indicate that Rivera variety is genetically different from the others.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: علي عبد الامير مهدي الصالحي
اسم المؤلف: هوازن حليم صالح

دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

المستخلص: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2013
الموضوع العام: علوم الحياة
اسم المشرف: احلام نعيم كاظم الياسين
اسم المؤلف: ايمان حمزة محمد

الكشف الجزيئي عن بكتريا Oxalobacter formigenes باستخدام جينات 16SrRNA، oxc في البراز من اشخاص اصحاء Molecular Identification of Oxalobacter Formigenes Bacteria Based On 16S rRNA, Oxc Genes In Stool Samples Healthy Subjects

المستخلص: بكتريا Oxalobacter formigenes من مكونات المكنون البشري الميكروبي، تتغذى على الاوكزالات السامة وبذا تخلص الجسم منها. البكتريا سالبة لصبغة كرام، ولا تكون الابواغ ولاهوائية المعيشة، ويمكن عزلها من الغائط البشري، ولدراسة البكتريا من النواحي الجزيئية، تم جمع ع | Oxalobacter formigenes is one of the human microbiome, uses the toxic oxalate for their growth (Oxalotroph), and helps the body to get rid of excess oxalate. The bacterium is Gram negative, non - spore former, obligate anaerobe, and can be isolated from human stool.To carry out molecular studies on this bacterium, 50 samples of human stool were collected from subjects with wide range of ages (3 - 63 years) and different genders, subjects were healthy and have no medications and especially antibiotics for at least for 3 months. Genomic DNA was extracted with special kit, to amplify certain housekeeping genes, these are 16S rRNA (3' end) segment having the anti - Shine - Dalgarno sequence (ASD), and the oxc gene responsible for production of oxalyl - CoA decarboxylase (middle region responsible for active site at where the Thiamin pyrophosphate binds, the enzyme co - factor). It was possible to amplify the 16S rRNA gene in 46 sample out of 50 (92%), and 7 samples out of 50 (14%) for oxc gene. Analysis and alignments of 16S rRNA sequences put the Iraqi samples in the O. formigenes i.e., the same species depending on Distance Score of alignments, it has been found that ASD of bacteria is conserved and represented by the sequence " 5' CGCGGUGAACGUUCCCGGG3' " in the studied samples and in strains found in the International Databases. Segment of oxc (~ 600 bp) translated into proteins (190 - 194 amino acids) were aligned with oxc protein of O. formigenes (M77128) reference strain, it has been found that this segment similar to the TPP binding site and characterized by its high hydrophobicity
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: زهرة محمود الخفاجي
اسم المؤلف: طارق طلال خلف

المراتب التصنيفية للنباتات البرية من ذوات الفلقتين في منطقة صدور - ديالى Wild Dicots Plant Taxa of Sudoor - Diyala Province

المستخلص: This study examined the current survey of the vascular plants of dicotyledon wild during the study period (2013 - 2012) In the area of Sudoor - Diyala, the study was based on the 774 samples of plants with their duplicates as collected by The Researcher. All of these collections have been cacched in the Iraqi herbariums. To be specific there are 186 species which relate to 133 genus and 43 families. All these, have been scientifically classified with thier local and common Iraqi or arabic names.Besides, their duration and economic importance (medical, toxic, nutritional or forage, artificial, harmful, aromatic, ornamental, fuel plants or other uses). Moreover, their geographical distribution in the districts of Iraq and thier geographical existence in neighboring countries to Iraq (Saudi Arabia, Kuwait, Turkey, Iran, Syria and Jordan) , and the study results statistics have shown that the vast majority of the collected species are Herbal Plants totalling 164 species out of 179 species in all.This, in fact, can be ascribed to the impossibility of txonomizing 7 plants species for the incapacity to obtain their flowers or fruits. As for the shrub species it amounts to 13 species where as the number of woody species of trees is 2 only. As for the economic plants and their significance, let s mention the fact that the number of plants used as forage amounts to 86 species, the medical plants are 83 species, the toxic plants fall into 43 species, the ornamental plants are 23 species, the industrial plants are 14 species, the weed plants are 11 species, the aromatic plants are of 6 species, and the plants used as fuel are of 4 species. It also appears that the majority of the plant in the area of study is widespread in the various districts of Iraq, i.e. the number of such plants amounts to 110 species, however, there are plants of medium spread in the districts of Iraq, i.e. the number of such plants amountsto 54 species.There still are some plants whose geographic distribution has no been identified in the districts of Iraq and their number is 15 species, and as for their geographic extension into the neighboring Countries, these plants amount to 116 species in Saudi Arabia, 84 species in Turkey, 81 species in Iran, 79 species in Kuwait, 69 species in Syria and 26 species in Jordan. Furthermore, the species of plants collected perviously and have not been found during the period of study amount to 32 species and these relate to 13 families.The shrinkage of the vegetation cover in the area of Sudoor is attributed mainly to the drought conditions and the wind soil erosion which the area has undergone in the previous years. Besides, there are some other reasons including the overgrazing, the expansion of agriculture and the unstudied projects of construction, which have resulted in the disappearance of natural plants in the area.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: علي حسين عيسى الموسوي وسام مالك داود
اسم المؤلف: نسرين صبار هاشم حسين المهداوي

دراسة المشعرة المهبلية Trichomonas vaginalis والجراثيم المرافقة لها في نساء مدينة تكريت Study of Trichomonas Vaginalis And Bacteria Associated With It in Tikrit City's Women

المستخلص: تناولت الدراسة الحالية عزل طفيلي المشعرة المهبلية T. vaginalis والتعرف على انواع البكتريا المرافقة له من النساء اللواتي يعانين من التهاب المهبل Vaginatis المراجعات لمستشفى تكريت التعليمي قسم النسائية والتوليد للمدة مابين اب 2007 ولغاية نهاية شباط 2008،بلغ | The present study included the isolation of T. vaginalis and conducted to determine bacteria associated with this parasite in women attending gynecology and delivary department in Tikrit teaching hospital suffering from vaginitis from august 2007 till the end of February 2008. One hundred ninety three samples were collected, their ages ranged between (14 - 57) years.The rate of infection with this parasite was 9.3%. it was not associated with bacterial in a proportion 33.3%, where as 66.7% was associated with bacterial infection.Bacterial vaginitis represented 80.4%, S. aureus was the most commonly isolated associated with T. vaginalis. It was with a proportion 41.8% and was the most common bacteria represented 21.3% among other bacterial species causing vagintis. The sensitivity of diagnostic tests were variable since the direct examination was the highly sensituse test (100%) in comparison with staining using giemsa and gram stains, their sensitivity were 19.6% and 0.0% respectively. The most common clinical symptoms related to trichomoniasis infections were discharges with burning and itching which represented 44.8% and white discharge was 64.7% which represent discharge according to color.. According to epidemiology of this parasite, the highest percentage of infection was 14.6% in urban women with secondary educational level and with high socioeconomic level while represent 22.7% and 15.8% respectively and the highest rate of infection (12.9%) as recorded in ages group (24 - 33) years. In married women the proportion of infection was 9.4% while in women whom were not child bearing it was 15.9%. Also a highest infection rate 16.6% was recorded in women who using antifertility tablets. The present study also determined the relationship between the pH of vagina and types of infection since high infection rate was with parasite 91.7% and 97.4% of bacterial vaginatis at pH ? 4.5. The present study showed a highly significant elevation in E2 hormone valvo in pregnant and nonpregnant women infected with trichomoniasis while this hormone was decreased in sterile women infected with trichomoniasis and in pregnant and nonprengnant and sterile women infected with bacteria vaginatis.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: حسين ساهر اسود العبيدي عبد الله حسين عبد الله الجبوري
اسم المؤلف: ميلاد عدنان مزهر

تاثير نقص البروتين في بعض التغيرات النسجية والسريرية والوظيفية في ذكور الفئران البيض The Effect of Protein Deficiency In Some Clinical, Histological And Physiological Changes In Male White Mice

المستخلص: The present study is designed to know the effect of protein deficiency on mice males through study the clinical histological changes of some organs including (liver, kidney, stomach and testes).In addition of study the blood and biochemical parameters, where they were sheltering animals in the animal house of the branch of physiology / College of Veterinary Medicine / University of AL - Qadisiya and the number was 90, all of the mice are males and age (28 - 30) days kept for two weeks before starting the experiment for the purpose of acclimatization was given during this period, diet control, then the experiment began between 01/05/2014 until 19/2 / 2014, and at weights ranging from start racing experience between 17 - 18 g, all in a period of growth.These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different period as follow (15, 30, 45)day.Experiment takes study of changes in body weight of animals and some selected organs also study histological changes for studied organs, also physiological blood parameters such as PCV, Hb, GCS and TSP.Results showed significant decrease (p<0.05) in weight body rate of animals, also in the rate of selected organs means compared with control group. The second treatment for 45 days most significant in decreased of body weight and organs weight if it compared with first treatment in all periodsHistological procedures showed that clinical and histological changes are graduated by the severe of protein deficiency and period of time. The histology investigation for liver showed that congestion of blood vessels accompanied with dilation of central vein and portal vein, and necrosis of the hepatic cells and destroyed the general total structure of liver. The section of kidney showed that necrosis and destroyed in renal tubules and epithelial cells in the lumen of tubules and bleeding in renal tubules and clear shrinkage in glomerulus. There are histological changes in stomach by necrosis and desquamation for gastric cells in both period (30, 45) days, while there are no histological clinical changes in both doses in period (15 days) for exposure. Histological sections of testes showed induction inhibition of spermatogenesis and desquamation of germ cells and congestion in the interstitial tissue and dilation of lumen of seminiferous tubules in both periods (30, 45) days, while there is no any changes in both first and second treatment in period of (15)days.In contrast, the hematological parameters showed that the concentration of Hb, PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.Biochemical tests obtain significant decrease (p<0.05) in blood glucose level in both treatment first and second in first period. Also the results showed that no significant increase in first treatment in both periods (30, 45) days compared with control group, while there is significant increase in the level of blood glucose in the second treatment in both periods (30, 45) days compared with the control group. Also did not score a significant decrease (P <0.05) in the total serum protein in thefirst treatment in the first period (15) days, compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while found a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group.It can be conclusion that the exposure for protein deficiency due to clear clinical histological changes for studied organs and some changes in some hematological and biochemical parameters in white mice which treated with protein deficiency which it causes these symptoms
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: علوم الحياة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: فاضل فرهود مكي الجبوري هاشم محمد عبد الكريم العلاق
اسم المؤلف: نسم عماد دايم الفؤادي

تاثير المستخلص المائي الخام لنبات الزنجبيل Roscoe officinale Zingiber على بعض الجوانب الدموية والتناسلية في الفئران الحوامل Effect of Crude Aqueous Extract of Zingiber Officinale Roscoe On Some Hematological And Reproductive Aspects In Pregnant Mice

المستخلص: اجريت هذه الدراسة للتعرف على مدى تاثير المستخلص المائي الخام للزنجبيل Zingiber officinale Roscoe (الطازج، اوالجاف) على الفئران الحوامل المجرعة عن طريق الفم وبالتراكيز (284، 568، 1136، 1420 ملغم/ كغم) اثناء مراحل الحمل المختلفة (المبكرة والوسطية والمتاخرة | This study was designed to investigate the effect of the crude aqueous extract of ginger (Zingiber officinale Roscoe) (fresh or dry) on pregnant mice orally administered at doses (284, 568, 1136, 1420 mg/kg) during different durations of pregnancy (early, intermediate, and final) for a period of 7 days for each duration. The study showed that there was no significant difference in the average weights of the animals at (P < 0.05) after treatment with (dry or fresh) extract at doses (284, 568, 1136 or 1420 mg/kg) comparing with the control group.The parameters of blood in the early duration of pregnancy showed a significant decrease in the average of hemoglobin (Hb) at (P < 0.05) after treatment with (fresh or dry) extract at the doses (568, 1136, 1420 mg/ kg), while the average of packed cell volume (PCV), demonstrated a significant decrease after treatment with fresh extract at doses (284, 568, 1136, 1420 mg/kg), and at (568, 1136, 1420 mg/kg) after treatment with dry extract at (P < 0.05) : The average of red blood cells (R.B.C.) after treatment with (fresh or dry) ginger extract, at (P < 0.05) showed a significant decrease at doses (284, 568, 1136, 1420 mg / kg), while the average mean corpuscular hemoglobin (MCH), and the average mean corpuscular volume (MCV) at (P < 0.05) revealed a significant increase and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract, at doses (1136, 1420 mg/kg) for the average of (MCH), at doses (568, 1136, 1420 mg/kg) for the average (MCV) after treatment with fresh extract, while the average Mean corpuscular hemoglobin concentration (MCHC) did not show any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the intermediate duration of pregnancy showed a significant decrease at (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, while dry extract demonstrated a significant decrease in average of (Hb) and (PCV) at doses (568, 1136, 1420 mg/kg), and a significant decrease in the average of (R.B.C.) but at doses (284, 568, 1136, 1420 mg/kg), while the average of (MCH) and (MCV) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while the average of (MCHC) did not showed any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the final duration of pregnancy revealed a significant decrease (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while (MCH) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, (MCHC) did not demonstrated any significant difference at the level (P < 0.05) after treatment with (fresh or dry) extract at all doses, while (MCV) showed a significant increase at (P < 0.05) and at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract comparing with the control group. The results showed a significant decrease at (P < 0.05) in the average of diameters of ovarian and corpus luteum after treatment with (fresh or dry) extracts in the early and final durations of pregnancy at doses (568, 1136, 1420 mg/kg), and in the intermediate duration after treatment with (fresh or dry) extract but at doses (284, 568, 1136, 1420 mg/kg) comparing with group control.As for the concentration of the hormone estrogen (? - estradiol) didn’t demonstrated a significant difference at (P < 0.05) during different durations of pregnancy after treatment with (fresh or dry) extract at all doses, whereas progesterone showed a significant decreased at doses (284, 568, 1136, 1420 mg/kg) in all durations after treatment with (fresh or dry) extract comparing with the control group. Using doses at (284, 568, 1136, 1420 mg/kg) with (fresh or dry) extracts revealed a significant increase at (P < 0.05) in the average number of infected follicles in the all durations of pregnancy comparing with the control group. The results showed that there are no significant difference in the lengths of the embryos during intermediate and final durations at (P < 0.05) after treatment with (fresh or dry) extract at all doses comparing with the control group.There are also histopathological changes in the ovary, uterus and placenta included degeneration in ovarian follicles, necrosis, congestion, hemorrhage, edema, vasodilatation and rupture in blood vessels at different using doses.These findings indicated that the treatment with (dry and fresh) extract at doses (568, 1136 or 1420) mg/ kg harmful on pregnancy in different durations (early, intermediate, and final) , also the using of fresh ginger more harmful than dry ginger.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: محمد عبد الهادي غالي
اسم المؤلف: رغد خالد موفق مسلم

التحري عن جينات المقاومة لمضادات البيتالاكتام واسعة الطيف في بكتريا Acinetobacter baumannii المعزولة من مصادر سريرية Detection of Extended Spectrum Beta - Lactam Resistance Genes In Acinetobacter Baumannii Isolated From Clinical Sources

المستخلص: تضمنت الدراسة عزل وتشخيص 16عزلة من بكتريا Acinetobacter baumannii من اصل 196 عينة جمعت من مصادر سريرية مختلفة في مستشفى بعقوبة العام ومستشفى البتول التعليمي وكانت اعلى نسبة عزل لهذه البكتريا من مسحات الجروح %10.8، ومسحات الحروق بنسبة 8.3%، والادرار 6.9%، | The study included isolation and diagnosis of 16 isolates of Acinetobacter baumannii out of 196 samples collected from different clinical sources in Baquba General Hospital and Al - Batool Teaching Hospital. The highest rate for the isolation of these bacteria from wounds 10.8%, burns 8.3%, urine 6.9%, and blood 5%. The diagnosis of isolates was confirmed by ViTEK2 device in addition to phenotypic, microscopic characteristics and biochemical tests.The results of the investigation of virulence factors for Acinetobacter baumannii isolates showed that all isolates have the ability to adhesion surfaces of epithelial cells of humans (100%), while the ability of 13 isolates to produce biofilm was 81.2%, seven isolates had possessed efflux pumps with high efficiency (43.7%). The investigation of the sensitivity of the isolates for ten antibiotics, the results showed all isolates were resistant to antibiotic cephalexin was 100%, against antibiotics meropenem and imipenem was 50%, the other antibiotics resistance ratios ranged between 81.2% - 56.2%. The results of multi - drugs - resistance tests showed ten isolates of Acinetobacter baumannii possessed of multiple antibiotic resistance, the isolates were divided into two groups, resistance group and other sensitive depending on the resistance to antibiotics, as the first group included ten isolates resistance from 6 - 9 antibiotics, the second group included six isolates resisted from 1 - 4 antibiotics, and the first group is dominant in the study. The concentration (100 µg/ml) of cephalexin, cefotaxime, ceftriaxone to test the ability of isolates were resistant to cephalosporin antibiotics, all isolates showed resistance to this concentration for antibiotic Cephalexin, the resistance Cefotaxime, Ceftriaxone to this concentration was 75% for both antibiotics. The combination disk method used to investigate isolates producing for extended spectrum ? - lactamase enzyms and metallo ? - lactamase enzyms the results indicated that the percentage of isolates under study producing extended spectrum ? - lactamase enzymes and metallo ? - lactamase were 62.5% and 56.2% respectively from the total 16 isolation. It was determined the minimum inhibitory concentration MIC for cefotaxime and ceftazidime by method of multiplying serial concentrations, The results showed that the minimum inhibitory concentration for cefotaxime values ranging between 32 - 1024 µg/ml, as for the MIC ceftazidime his values ranging between 16 - 1024 µg/ml. Conducted process extract bacterial DNA for isolates of Acinetobacter baumannii, then held polymerize chain reaction PCR for isolates resistant to antibiotics ? - lactam and MIC values more than 64 µg/ml through the use of specialized primers that target specific sequence of genes blaCTX - m and blaSHV, relay outputs of the multiplication agarose gel concentration of 1%, the results showed that 5 isolates of Acinetobacter baumannii out of 10 isolates possessed gene blaCTX - m and by 50%, as for blaSHV gene the results showed that three of the isolates under study only has this gene and by 30%.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عباس عبود فرحان الدليمي
اسم المؤلف: صفا ماجد محمد الباجلاني

التاثيرات النسجية والدمية وبعض النواحي الوظيفية لعقار البيروكسيكام في ذكور الفئران البيض Mus musculus Histological And Hematological Effects And Some Physiological Aspects of Piroxicam In Male Albino Mice Mus Musculus

المستخلص: The current study aimed to detect the effect of Piroxicam in making behavioral, hematological and physiological changes in the kidneys of adult male albino mice Mus musculus, The present study also included measuring the diameters of glomeruli, proximal and distal convoluted tubules as well as the histopathological study.A total number of (128) adult male albino mice were utilized in this study, they were randomly distributed into four main groups, the first three groups were orally treated with concentration (50, 100, 150) mg/kg respectively, the forth group considered as control group were orally treated with (0.1) ml of distilled water for eight weeks, the samples were collected every two weeks for the histological study, blood samples were withdrawn for the hematological and physiological studies.The current study showed that there were behavioral changes observed on the experimental animals, represented by reluctance to food intake, lethargy, tremor, introversion, fast breathing, change in eye’s color and blackened feces, and the intensity of these symptoms increases with the increment of concentration and administration period.The statistical study results revealed that there was significant decrease at (P<0.05) in the mean numbers of red blood corpuscles, hemoglobin concentration and the percentage of packed cells volume in orally treated groups with (50, 100, 150) mg/kg for (6, 8) weeks of administration in comparison with control group, as for the white blood cells the statistical results for this study showed significant increase (P<0.05) in their mean number in treated groups with concentration (100, 150) mg/kg for (6, 8) weeks of administration compared to the control, whereas the groups treated with concentration of (50) mg/kg showed no significant changes along the administration period.Also all the concentrations showed significant differences (P<0.05) in the mean concentration of urea, creatinine and potassium ion in the blood serum of orally treated mice at the last weeks of administration in comparison with the control group.The statistical results of the present study also revealed that there were significant differences (P<0.05) in the mean diameters of renal glomeruli of the treated groups with concentration (50, 100, 150) mg/kg for eight weeks while there were non - significant differences in other periods, also there was non - significant decrease in the mean diameters of proximal convoluted tubules in the treated groups with concentration of (50, 100, 150) mg/kg in all periods except the period four weeks it showed a significant decrease (P<0.05) in the mean diameters of proximal convoluted tubules of the groups treated with the concentration (100, 150) mg/kg in comparison with control group. The statistical results also clarified that there were significant differences (P<0.05) in the mean diameters of distal convoluted tubules of the treated groups with concentration (100, 150) mg/kg for six and eight weeks of administration, while the group treated with concentration (50) mg/kg had no significant differences over the whole administration period.The study also recorded the presence of histopathological symptoms in the kidneys of treated mice with the dose (50)mg/kg represented by hemorrhage, vascular congestion, calcium casts formation inside the lumens of renal tubules, while the groups treated with (100)mg/kg of the drug showed a fibrin deposition inside renal tubular lumens, infiltration of inflammatory cells, cytoplasmic vacuolation in the epithelial cells of the renal tubules, and sloughing epithelium of renal tubules, while in the medullary region, there were epithelial cells atrophy in the renal tubules. The histological examination of mice kidneys treated with (150) mg/kg showed the appearance of hyalinization, shrinkage and complete loss of glomeruli, Pyknosis of nucleus and fibrosis around the blood vessels as well as the presence of oedema which occupied a large area of the kidney tissue
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - الانسجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: انتظار محمد مناتي
اسم المؤلف: بشرى عمر احمد معروف

تقييم نوعية المياة الصناعية ووحدات المعالجة لمعمل النسيج القطني في الكاظمية Evaluation of The Industrial Wstewater And Treatment In Al - Kadhimia Cotton Weaving Industry

المستخلص: This study includes two axis, the first one determined the physical and chemical characteristics for several concentrations of industrial wastewater of cotton textile factory, that located in Al - Kadhmiya, which are disposable to the main assembly in the treatment unit (first unit) of the factory ; Also the final water quality was evaluated after the treatment in the fourth unite and compared with normal environmental averages (or compared with acceptable limits for discharged.The second axis of this study assessed the efficiency of the physical and chemical treatments in the third unit, and also the biological treatments in the fourth unit through detecting the removal percentage for each stage of the treatment to evaluated the treatment unit efficiency of this factory. Samples were collected from four treatment units for six month, single sample was collected per two weeks.The (local, field) tests were assessed for the collected samples including (Temperature, PH, DO and EC, while the laboratory tests included TSS, TDS, BOD, COD, oil and grease, So4 - 2 and heavy metals such as Pb, Zn and Cr).The results showed that the temperature values were with (normal range) acceptable limits (22.3C?) in the first unit and (19.8C?) in the fourth unit; PH values also were within normal limits in the almost treatment units (neutral), however, the electrical conductivity average was 3123?c/cm in the first unit, and 1101 ?c/cm in the fourth unit ; Moreover, the average values of COD were 580 mg/L in the first unit and 163 mg/L in the fourth unit. The removal percentage was 41% in the primary sedimentation in thesecond unit while it was 36% in the third unit for biological treatment.The BOD values were 487 and 302 mg /L in the first and fourth unit respectively ; the removal rates were 27% and 29% for primary sedimentation stage and biological treatment respectively and these rates are acceptable for normal limits.In general, the final treatment concentration was 302 mg/L in the fourth unit which is normal environmental rang, and also BOD concentration was not exceeded the environmental limits in the final stage of the treatment while the total percentage dissolved salts were 37% and 22% for the third unit which are normal in comparison to the Iraqi limits after the treatment for the fourth unit (703mg/L). Furthermore, the results of suspended solids found that the removal percentage was 76% for the second unit and 43%for the third unit while the total concentration of the last treatment was 61mg/L which is with normal limits, also the concentration of lipids of oils and grease was 0.8 mg/L for the fourth unit, while the removal percentage was efficient for the primary sedimentation and biological treatment. The heavy metals concentraitions were now with water and the main assembly tank and this was within normal environmental limits.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة - التلوث البيئي
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: سهير ازهر الكاظمي
اسم المؤلف: اسيل حسين محمد السعداوي

دور اجهاد المضاد الحياتي (سيفتازديم) في امراضية الممرضة البولية الايشريكية القولونية المنتجة للبيتا لاكتاميز موسعة الطيف في الفئران المختبرية Role of Antibiotic (Ceftazidim) Stress On Pathogenicity of Uropathogenic Extended Spectrum ? - Lactamaes E. Coli In Laboratory Mice

المستخلص: جمع 60 نموذجا من عينات بول وسط المجرى من المرضى الراقدين في المستشفى الذين يعانون من اخماج المجاري البولية من مستشفى اليرموك ومستشفى الكرخ ومستشفى القديس رافائيل/ الراهبات للمدة من 27 شباط 2014 ولغاية 25 اذار 2014، وقد اظهرت نتائج الزرع البكتريولوجي الحصو | Sixty mid - stream urine specimens were collected from patients presented with urinary tract infections whom attending Al - Numan, Al - Yarmouk, Al - Karkh, and Saint Raphael/Al Rahibbat hospitals in Baghdad for the period February, 27th to March, 25th 2014. Bacteriological culture identified fifty (80.33%) isolates as E. coli depending on cultural and biochemical characteristics as well as Api and Vitek 2 automated systems. However, 82% of isolates developed ? - hemolysis on blood agar plates.Susceptibility of all isolates against seven commonly prescribed antibiotics were investigated and 96, 82, 84, 92, 92, and 56%. of isolates were resistant to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefalothin, imipenem, and meropenem, ceftazidime, respectively.Concomitantly, ? - lactamase production was detected phenotypically by disk replacement method. The results showed that 82% of all isolates have the ability to produce ? - lactamase. Interestingly, correlation coefficient between ? - lactamase and hemolysin production was 0.99.Using microtiter plate method, all E. coli isolates were tested for their ability to form biofilm. The results revealed that 36, 54, and 10% of isolates formed biofilm with variable thickness; weak, moderate, and strong, respectively.Alongside with previous findings, blaCTX - M - 2, blaVIM, blaTEM, and blaPER were identified in 18, 12, 10, and 4, respectively. Nevertheless, none of blaOXA - 1, blaOXA - 4, blaOXA - 30, blaIMP, blaCTX - M - 1, blaCTX - M - 9, blaKPC, and blaSHV. The isolate number 11 harboured the highest number of genes (blaTEM, blaVIM, and blaPER) among all tested isolates. Twelve albino mice was divided randomly into four groups comprising A through D injected with ceftazidime at sub MIC, E. coli 11, E. coli 11 with ceftazidime solution, and standard strain, respectively.Histopathological sections did not show any changes in respeuct to group A. however, group C suffered signs of infection less than those appeared in group B sections. Simultaneously, group D suffered intense histpathological changes more than other groups infected with resistant isolate.
الصفحات الاولى:
مكان الجامعة: الانبار
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: محمد قيس العاني حارث جبار فهد
اسم المؤلف: علي حسون حمادي عبد الله

دراسة علاقة سم Ochra. A بحالات الفشل الكلوي غير معروف الاسباب في محافظة القادسية Study The Relationship of Ochra.A With Kidney Failure of Unknown Case In Al - Qadisiya Governorate

المستخلص: اجريت هذه الدراسة لتحديد العلاقة بين السموم الفطرية وحالات الفشل الكلوي غير معروف الاسباب لدى المرضى المراجعون الى مستشفى الديوانية التعليمي للفترة من اذار/2012 الى كانون الثاني/2013 والذين تم تشخيصهم من قبل الاطباء المختصون. اظهرت نتائج فحص عينات الادرا | This study was designed to detection the Relationship between some fungi and it’s toxin with Renal falier in patients suffer from Renal falier attended to Al - Diwanyia teaching hospital in period from March/ 2012 to January/ 2013, These patients were diagnosed by physician in above hospital. By using Thin Layer Chromatography (TLC) the results showed 23.07% from patients blood specimens had Ochratoxin A, 19.23% from patients urine specimens had Ochratoxin A 38.46% from patients had Ochratoxin A in blood and urine where only 59.61% your blood and urine were clear from above toxin, So healthy persons were had Ochratoxin A in your blood and urine by rate 20% 40% respectively and 10% from them had Ochratoxin A in your blood and urine and only 65% from them don’t had Ochratoxin A in their blood and urine.the concentration of Ochratoxin A in blood were ranged between 6.2 - 25.8 ?g/kg from weight boody and 4 - 10.3?g/kg in urine and in healthy reach concentration of Ochratoxin A in blood 2.3 - 3 ?g/kg from weight boody and 4.3 - 4.6 ?g/kg in urine.The data of this study showed the male highly infected 87.5% with ocratoxin while in female 70% and 71 - 80 years age had highly infected 92.85% with ochratoxin A. The results of study of biochemical parameter in patients showed highly raised in WBC and urea and Keratinin to reach to 12000 corpuscle /ml3 310 mg/dl, 12.2 mg /dl respectively and lowed the activity of alkaline phosphates enzyme and albumin into 2.2 u/l, 1.5 gm/dl, respectively and suger 120 mg /dl. The results of isolation and diagnosis by depending on Morphological, Microscopical and Biochemical characters showed there were two species of fungi belong to Aspergillus sp, include A. ochraceaus 25.42%, A. niger 12.71% and A. candidus 15.25% and Candida albicans 46.61% were isolated from urine patients where only C. albicans and C. krusi were isolated from blood of patient by percentage 66.66% and 33.33% respectively. So the results of single PCR method selected to detection the OCR and PSK genes in Aspergills spp which responsible for ochratoxin A production , showed 83.33% from A. ochraceaus isolates had OCR gene (907 bp) and 66.66% from A. nger had PSK gene (584bp)Also the results of this study showed A. ochraceus and A. niger had ability to produce metabolic compounds when grow on yeast - extract broth and these compounds had significant toxic effects(P?0.05) in biochemical parameters in albino rats represented raised number of white blood cells (WBCs) into 10610 cell/µm3, urea into 228 mg/dl and Keratinin into 15 mg/dl in blood of animals which treated with 5000 ?l/kg from metabolic products of above fungi respectively, comparative with control groups 10100 cell/µm3. in other side, these metabolic products caused in same dose lead to lower of albumin and activity of alkaline phosphatase enzyme into 2.8 mg/dl and 3.7 u/l respectively where normal level for this parameter in control groups.Histological study showed the dose of metabolic products 500 - 5000 µl/kg for both fungi caused pathogenic changes in kidney represented by, atrophy highly proliferation of cells in endothelial layer of Bowman's capsules in addition to hemorrhage and Focal agrgregate inflammatory cells.In other side, study of toxicity of ochratoxin A which extracted by using thin layer chromatography (TLC) teqnique from urine and blood of patients and yeast extract broth media that had growth of A. ochraceus and A. niger showed signification effects(P?0.05) for this compound in physiological and biochemical parameters also caused significant effects in histological parameters similar to these effects which occurred in previsious experiments while these effect don’t appear in control groups.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دكتوراه
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: بهيجة عبيس حمود الخالدي
اسم المؤلف: هدى رحيم هاشم الموسوي

تحديد القابلية التمنعية لمستضد الفوعة لبكتريا (Vi) Chitosan المحملة على حبيبات Salmonella typhi وذيفان الكزاز في الجرذان Determination of Immunogenicity For Virulence Antigen of Salmonella Typhi Antigen Conjugated To Chitosan And Tetanus Toxoid In Rats

المستخلص: This study was carried out during the period from October 2013 to September 2014. Salmonella typhi Samples were obtained from the Central Health Laboratory for the purpose of purification capsular polysaccharide antigen (Vi Ag). Capsular polysaccharide antigen was loaded on chitosan nanoparticle and tetanus toxoid. Recorded results showed that the antibody titers in (T1) Vi Ag + chitosan groups(620.8±364.8) were increased significantly than other groups, it included (T2) Vi Ag group (448±117.73), (T3) Vi + Tetanus toxoid group (537.6±142.53) and (T4) Vi Ag+ Ch. +TT group (121.6±38.4) at (P? 0.05).The documented results of total and differential WBCs counts were showed a significant increment in group (T1) (21020 ± 891.85 cell/1ml) compared with the control group (C) (5500 ± 230.94 cell/1ml) and other groups, it included (T3) group (10740 ± 980.61 cell/1ml), (T4) group (10650 ± 1217.78 cell/1ml) and (T2) group (9980 ± 572.18 cell/1ml) at (p? 0.05). on the other hand, the results of differential count showed elevation in percentage of neutrophils in group (T2) (40 ± 1.89%) compared with the control group (C) (33.3333 ± 0.66%) and other group, it included a (T3) group (33.6 ± 0.67%), (T4) group (34 ± 1.37%) and (T1) group (19.4 ± 0.66%) at (p? 0.05). Results also showed an increase in lymphocytes in the group (T1) (79.2 ± 2.7%) compared with the control group (58.3333 ± 1.76%) and other group, it included (T4) group (62 ± 2.06%), (T3) group (61.2 ± 2.72%) and (T2) group (55.8 ± 1.11%) at (p? 0.05).Also The study included determination the level of gene expression of two clusters of differentiation, CD25 and CD29 gene expression by RT - qPCR. The results of relative gene expression in CD25 gene showed in T4 (5.1936±2.17) were increased significantly than other groups, it included T1 (2.5604±0.64), T2 (3.7032±0.54) and T3 (4.4739±1) at (P? 0.05). The results of relative gene expression in CD29 gene which showed T3 (162.3256±89.52), increased significantly than other groups, it included T1 (33.8276±16.6), T2 (59.6817±26.65), and T4 (37.9605±3.09) at (P? 0.05).This findings were supported by the increment activity determined in lymph nodes of spleen & thymus which increased in size and numbers with active germinal centers. The overall results indicated that the use of chitosan nano particles and tetanus toxoid can enhance and improve the immune response against a given vaccines.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: زياد متعب الخزاعي
اسم المؤلف: رغدة سعد محمد العمري

دراسة معالجة مياه الفضلات لمصافي بيجي وامكانية استخدامها كمياه لري بعض الخضر Study of Wastewater Treatment for Baiji Refinery And The Possibility of Using It As Water Some Verdures

المستخلص: اجريت الدراسة في قسم علوم الحياة - كلية التربية - جامعة تكريت من شهر تشرين الثاني 2010 الى شهر تموز 2011 ? وكان هدف الدراسة هو معرفة بعض الصفات الفيزياوية والكيمياوية للمياه المطروحة من شركة مصافي الشمال/بيجي وامكانية استخدامها لري بعض المحاصيل الزراعية ب | This study was conducted at the Department of Biology in the College of Education - University of Tikrit. The study aims at estimating some physical and chemical characteristics of water raised from the North Refineries Company / Baiji and can be used to irrigate some crops after treatment with a number of physical methods. It also aimed at estimating some heavy elements and their impact on the growth of plants that have been studied (tomato, eggplant, sweet pepper, hot pepper). The region from which samples have been brought of the area was Albu Juwaree village that is located in the city of Baiji and adjacent to the company. Besides, it occurs near wide agricultural areas. After estimating some physical and chemical characteristics of water, soil, and the proportion of oil waste, qualities of plants were studied as well as the assessment of (carbohydrates, proteins) and some of the heavy elements and their effects on the growth of those plants.The results showed that the water of industrial waste led to a reduction in the proportion and natural growth of plants. The most affected seeds were the seeds of sweet pepper, where the total percentage of germination was(0%) when infected %)100) and the least affected seeds were the seeds of tomato, where the total percentage of germination seeds was (80%) when water is contaminated (100%). The treatment by filtering has achieved the best germination of seeds of tomato and sweet pepper, where the ratio was (100%). It turned out that contaminated water has prevented the natural growth of those plants in respect to the number and the size of leaves, the length of roots and the weight of fresh and dry leaves. The methods of physical treatment showed that lightening (25%, 50%, 75%) made to the contaminated water led to the removal of the significant effects, especially when lightening was (75%).The results indicate that there is no significant differences in the lengths of the roots of tomatoes and eggplants, except for the root of hot and sweet pepper.While the heavy elements, which was part of the study in this research, beginning with cadmium was at its highest value at the treatment by filtering and less value in (100%). Through the study it was found that the percentage of lead in all studied plants were (0.00) ppm. The Results showed that the contaminated water led to the reduction of the proportion of carbohydrates with the increase of pollution(%100), where the least proportion of carbohydrates was at the treatment by filtering. The results also showed a decrease in protein concentration with the least proportion at lightening and less value in. (25%)The results showed that the water content of contaminated industrial heavy metals studied (cadmium - lead - copper - zinc - nickel) has decreased at all treatment when treated by filtering, as well as for the remaining elements, except lead, which was (0.00) in all plants and all lightening ratios.The results of this study show that treatment of contaminated water with industrial oil waste by physical methods led to minimizing the negative impacts of such water in reducing the percentage of germination and the reduction below the natural growth compared with non - treatment water and showed that the best way of treatment for the best growth of plants were lightening with (75%) and treatment by filtering. The best response to the treatment was tomato and the most resistant to pollution were (tomato and eggplant) and the most affected by contamination and least resistant was sweet pepper.
الصفحات الاولى:
الكلمات الدلالية:
  • البيئة - التلوث البيئي
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: خليل ابراهيم بندر النعيمي
اسم المؤلف: مريم عدنان ابراهيم

دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

المستخلص: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples
الصفحات الاولى:
مكان الجامعة: كربلاء
اللغة: العربية
الدرجة: ماجستير
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: هيام عبد الرضا كريم العواد ياسمين خضير الغانمي
اسم المؤلف: ابرار علي حسين

تاثير الاجهاد المائي والسماد الورقي والمخصب العضوي في نمو نبات حلق السبع الشجيري Adhatoda vasica (L.) Nees ومحتواه من بعض المواد الفعالة وفعاليتها المضادة في بعض بكتريا تسوس الاسنان Effect of Water Stress, Foliar And Organic Fertilizers On Growth of Adhatoda Vasica) L.(Nees. And Its Content of Some Active Ingredients And Inhibition Activity of Some Dental Caries Bacteria

المستخلص: This study was conducted in Biology Department - College of Science - University of Al - Qadisiya that included pot experiment from the period of 1/4 to 1/8/2013, to assess the effect of water stress, foliar fertilizer (Foltron) and organic fertilizer (Azomin) on growth of Adhatoda vasica (L.) Nees and Its Content of Some Active Ingredients. Laboratory experiments were also done from the period of 1/8 to 1/3/2014, to determine the activity of leaves and roots extracts that resulted from plants of pots treatments to inhibit growth of some bacteria genera causing Dental Caries i.e. Staphylococcus aureus, Streptococcus mutans and Streptococcus pneumonia.A pot experiment included seventy two transplants (age of one month and 8 cm height) that were transplanted in plastic pots (one transplant per pot) in 1/4/2013. Pots were filled with sandy soil and peatmose 2 : 1 v/v. Plants Irrigated with two levels 50 and 25% of field capacity. Foliar fertilizer was used at three concentrations of 0, 3 and 6 ml/L that sprayed twice on plant shoot till complete wetness during 15/4 and 1/6/2014. Organic fertilizer was also used with irrigation water at three concentrations of 0, 4 and 8 ml/L twice on 1/5 and 15/6/2014. The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (2×3×3) with four replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.05 level when treatment effect was significant.Results of pots experiment were as follows : 1 - Increasing water stress level caused a significant decrease in the most characteristics of vegetative and root growth except for stem internode and root lengths, while increasing in concentration of foliar and organic fertilizer gave the opposite effect of water stress.2 - Water stress caused a significant decrease in leaves content of total chlorophyll and nutrient elements N, P, K, Mg and protein percentage, while foliar and organic fertilizer caused a significant increase on the above characteristics.3 - Water stress caused a significant increase in proline percentage and Vit.C. and Vasicine and Vasicinone alkaloids, while foliar and organic fertilizer caused a significant decrease in proline percentage and Vit.C. and a significant increase in Vasicine and Vasicinone alkaloids.4 - The treatment of plants that were subjected to the water stress via foliar or root fertilizing or both of them caused a significant increase for most of vegetative and root growth characteristics, total chlorophyll, nutrient elements, protein percentage and Vasicine and Vasicinone alkaloids.5 - The treatment of plants that were underwent to the water stress via foliar and root fertilizing caused a significant decrease in proline percentage, Vit.C. and stem internode and root lengths.6 - The interaction of the three studied factors gave a significant positive effect to rise alkaloids of plant.Laboratory experiments included picking fresh leaves and root of plants that dried aerial in a shade, and then extracted it via methanol alcoholic of 70%, it was detected for some chemical components of extract by using reagents. Bacterial genera were isolated from the surfaces of the teeth and cultured on the growth media, and it was diagnosed by the Vitek Compact 2 System Diagnosis. The activity test involved the impact of the alcohol extract for leaves and roots to the inhibition of bacterial growth by the agar well diffusion method on Muller - Hinton.The design of the experiment was Complete Randomized Design (CRD) in a factorial arrangement (18×3) with three replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.01 level when treatment effect was significant.Results of laboratory experiment were as follow : 1 - Increasing extracts concentration caused a significant increase in growth inhibition of the studied bacterial genera.2 - plants extraction that of the combination of A2B3C3 included (25% water stress, 6 ml/L foliar fertilizer and 8 ml/L organic fertilizer) gave the highest activated inhibition for bacterial genera compared with plant other extraction for all tested bacterial genera.3 - The plants extracted that treated by combination A2B3C3 in concentration 150 mg/ml gave higher activated inhibition for Streptococcus pneumonia reached 25.307 ml compared with Amoxicillin that reached 36.167 ml, while the inhibited extracted root reached for the same treatment 16.060 ml (Table 37 and 38).4 - Plants leaf extract was the highest effective inhibition of bacterial compared to the roots extract.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: النبات - الفسلجة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ثامر خضير مرزة
اسم المؤلف: ليث سريع الركابي

عزل وتشخيص بكتريا Streptomyces المنتجة للمضادات الميكروبية من ترب بعض اقضية محافظة صلاح الدين Isolation And Identification of Antimicrobial Producing “Streptomyces” From Soils of Some Districts in Salahaddin Province

المستخلص: تضمنت الدراسة عزل وتشخيص انواع تابعة للجنس Streptomyces، اذ جمعت 50 عينة من ترب اقضية تابعة لمحافظة صلاح الدين. شخصت?11 نوع من بكتريا Streptomyces باستخدام الاختبارات الشكلية والمزرعية والكيموحيوية حيث ظهر كل من الانواع (Streptomyces antibioticus, Strep | The Study involved ; isolation and characterization of species related to Streptomyces. Fifty samples of soil has been taken from different districts of Salah aldden province. The isolates were identified by the morphological, biochemical tests and classified to nine species, The following species were found : (Streptomyces antibioticus, Streptomyces coelicolor , Streptomyces distalicus and Streptomyces fluvissimus ) in Tikrit district, ( Streptomyces cyaneus ) in Ballad and beiji districs, ( Streptomyces exofolicus) in Ballad and Aldor districts (Streptomyces lavendulea ) in Samarra and Aldor districts , (Streptomyces purpureus ) in Samarra and Beiji districts and (Streptomyces violaceus ) in Samarra and Tikrit districts. The sensitivity of these bacterial isolates were tested toward the following : (Azithromycin, Cefotaxime, Cephatexin, Doxycycline, Erythromycin , Gentamicin, Neomycin, Penicillin, Tobramycin and Vancomycin).All species showed high resistance to cefotaxime. But all species were sensitive to neomycin and doxycycline. The isolated species of Streptomyces were tested for their ability of antibiotic production. It was found that (Klebsiella) was sensitive to all the species except species Streptomyces coelicolor. Streptomyces coelicolor showed an effect against Proteus. While Staphylococcus aureus was sensitive to the following species (Streptomyces antibioticus, Streptomyces cyaneus and Streptomyces purpureus). The Scanning Electron Microscopy was used to study the surface of spores. The species of (Streptomyces antibioticus) was found to has warty surface while spores of ( Streptomyces coelicolor) has smooth surface.
الصفحات الاولى:
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: عبد الكريم فتاح عمر
اسم المؤلف: هيام عزيز عباس البدري

دراسة بعض المتغيرات الفسلجية وتركيز البروستاكلاندينات عند مرضى الربو

المستخلص: اجريت هذه الدراسة لمعرفة بعض المتغيرات الدموية والفسلجية عند مرضى الربو، وقد شملت 46 مريضا بالربو من كلا الجنسين (31 مريضا من الاناث و15 مريضا من الذكور) تراوحت اعمارهم بين 21 - 75 سنة من ضمن الحالات الواردة على مستشفى تكريت التعليمي والمركز الاستشاري لام
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: زيد محمد مبارك المهداوي صالح محمد رحيم العبيدي
اسم المؤلف: سيماء عبد الرحمن شعبان

معالجة مطروحات مياه فضلات الصرف الصحي لمدينة تكريت باستخدام تقنية المجففات الحرارية الدواره واعادة استخدامها كاسمدة عضوية Treatment of Domestic Wastewater Effluent of Tikrit City Using Thermal Rotating Dryer Technique And Reuse As Organic Fertilizers

المستخلص: اجريت الدراسة في مختبرات كليتي التربية والعلوم - جامعة تكريت للفترة من نيسان 2010 لغاية شباط 2011 تتضمن الدراسة معالجة الحماة الناتجة عن حوض التجميع الابتدائي باستخدام تقنية المجففات الحرارية الدوارة للعمل على ازاحة ماء الحماة والتي تمثل اصعب العمليات الت | This study was conducted at a period from April 2010 to February 2011, to treat the sludge produced from collection tank at tikrit domestic wastewater plant, using thermal rotating dryer technique(T.R.D) or dewatering the sludge.This process represents one of the difficult applied process for the sludge treatment. This technique considered a modern technique in this field.Physical, chemical and biological characteristics of the manufactured sludge were determined and compared with organic fertilizers (I.O.F) imported from republic of Estonia (Available in local markets).T.R.D technique induced dewatering of the treated sludge at ratio48.5 - 61.5% and producing highly sterilized organic fertilizers which was free of pathogenic organisms(bacteria, worms and eggs) with high chemical characteristics comparing with those imported from Estonia. Manufactured organic fertilizers M.O.Fs have allow acidity pH ranged between 7.5 - 7.7 ,. electrical conductivity at range 1630 - 1873, organic matter at ratio 4.56 - 5.35%, with a moderate concentrations of Pb, Cd, Zn.The concentration of N and K was 1.8, 5.96% respectively while the concentration of phosphorus are 350 ppm. Three levels of M.O.Fs were used for corn plants(5, 10 and 20ton/acres). Results showed that all these levels significantly induced increasing for some physiological and morphological characteristics of corn plants compared with those treated by I.O.Fs or control plants. The vegetative dry weight of corn plants fertilized by M.O.Fs were 23.95gm/plant compared with 16.3 and 5.3 gm/plant for plants fertilized by I.O.Fs and control respectively. (M.O.Fs) induced significant increase in nitrogen concentration compared with plants fertilized by I.O.F and control while the concentrations of Pb, Cd, Zn were not affected by using of M.O.Fs on the other hand Pseudomonas aeruginosa and Escherichia coli were contributed in reducing and removing some of pollutants from the sludge at three incubation periods (30, 60 and 90 day). These two species caused significant decrease in pH and EC of the sludge, P. aeruginosas showed the highest removing ratio of nitrogen (93.1%). The cooperation of the two species caused high removing ratio for the Total organic carbon(87.72%) at 60 days and organic materials (90.68%) at 90 days of incubation also showed high removing ratio for phosphors 100%) at 90 days of in cubation.) These two species also caused high removing ratio for pb, Cd and Zn at30 days and P. aeruginosa was more effective in removing Pb, Cd and Zn from the sludge then E. coli.
الصفحات الاولى:
الكلمات الدلالية:
  • البيئة - التلوث البيئي
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: البيئة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: جهاد ذياب محل الجنابي
اسم المؤلف: هالة ارشد علي حسين

انتاج انزيم الفوسفاتيز القاعدي من العزلة المحلية لبكتريا Bacillus sp. وتنقيته جزئيا

المستخلص: Fifteen local isolates of Bacillus bacteria and previously isolated were screening a from laboratory of advanced biotechnology and Genetic Engineering in Babylon University, College of Science to know their ability on production of extracellular alkaline phosphatase after its growth in liquid production in PH8 medium for 4 days and temperature 37°C. and destinguished 11 isolates by their different abilities on produc of enzyme and Bacillus sp.I bacteria was their best and perfected it selection to complete the study and described by depending on microscopic and cultured and biochemical characteristics studying of effect some of environmental condition for production of alkaline phosphatase enzyme by submerged cultural method were appeared that the optimum PH for production was 8, and optimum temperature 40°C, after incubation time 4days and explain that the production by static incubator was better from shaker incubator.Alkaline phosphatase of the isolate Bacillus sp.I was purified partial by fractionating with ammonium sulphate and the best ratio for saturation 95%. And on purification of enzyme by ion exchange chromatography with batch wise by using DEAE - cellulose and enzymatic recovery was 37.1%, and fold 3.64 once, and enzyme passed after concentrated on gel filtration column (Sepharcyl S - 200) one peak was appeared for enzyme and enzymatic recovery was 23 % and fold 36.80 once.Results of electrophoresis by presense of denaturation condition appeared that the molecular weight of alkaline phosphatase produced from gel filteration by using Sepharcyl S - 200 gain approximately 29.5 KDa and 28.8 KDa and this enzyme composed of homodimer.The characterization results for the enzyme indicated that the optimal pH for activity of enzyme was (9), while the optimal pH for the enzyme stability was (8 - 9), and found that the optimal temperature for activity of anzyme was 30 ? C, and on studying optimal temperature for stability of enzyme indicated that better stability for this enzyme was on temperature 0 ?C while the activity lost completely on temperature 60°C. and on studying effect some chemical compounds in activity of enzyme found that the enzyme needs to cofactor and it is zinc andmagnessium ion to increase catalytic activity and needs to calessium ion to increase stability of enzyme. At that time potassium and sodium ions appeared a negatively effect on activity, and heavy metals ions Fe and Hg appeared an inhibited effect to activity and so that the enzyme was inhibited by presence EDTA and and potassium ferro cyanide indicating that the enzyme from metal enzymes.Determination of constant kinitics of the enzyme was completed of Michaelis - Menten constant (Km) of para - nitrophenyl phosphate and this gained 500 micromolar and maximum velocity of this enzyme (Vmax), and this gained 2500 unit/ml.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: رباب عمران راضي الجيلاوي
اسم المؤلف: جعفر انور قدوري الخاجي

دراسة تشخيصية وتصنيفية لطفيليات الديك الرومي Meleagris gallopavo في محافظة القادسية Diagnostic And Taxoaomical Study of Turkey (Meleagris Gallopavo) Parasites In Al - Qadisiya Province

المستخلص: خلال المدة ما بين بداية شهر ايلول 2014 ونهاية ايار 2015 جمع وفحص 50 طيرا من الديك الرومي Meleagris gallopavo من مناطق مختلفة من محافظة القادسية في مختبرات الكلية بهدف عزل الطفيليات الخارجية والداخلية المتطفلة على هذه الطيور وتشخيصها فضلا عن دراسة نسبة الا | The current study carried out during the period between the first of the September 2014 end of May 2015.fifty birds of domestic turkey Meleagris gallopavo was collected from different areas of Al - Diwaniya city markets, to isolating and identification the external and internal parasites parasitized on these birds as well as study the incidence and intensity and the most important pathological and histological changes resulting from external and intestinal infections, Six species of external parasites was isolated included four species of biting lice Gonoiocotes gallina, Goniodes gigas, Menacanthus straminus and Oxylipeurus sp. The infection rate and severity of 10%, 6.81.4%, 4.72.2, %, 54.2%, 0.09, respectively, and Two species of Ticks are Argus persicus and Haemophysalis sp. Nymph. and the rate of infection was 2%, 0.36; 2%, 0.24 : respectively. The results showed that the triple infection of lice are the most common, (63.63%.) While internal parasites that 9 species of intestinal parasitic worms in the digestive system included two species of Protozoa Eimeria sp. and Histomonas meleagridis The infection rate and intensity was14%, 4.20 0.2%, 0.14, respectively, Four species of tape worms Railletina cesticillus, Railletina tetragona. Choanoyania infundibulum and Hymenolepis sp.The intensity of infaction was 2%, 100.2% 53.284, 4, 00, 2% 2%, 0.42; respectively, Three species of nematodes Ascardia gallinarum, Capillaria sp. and Heterakis gallinarum and severity of infection was 21.73%, 1.2; 2% 0.100; 17.79%, 1.25; respectively. The study showed the presence of internal parasitic worms sites in the gastrointestinal tract it was observed that most of tapeworms parasitize in the small intestine and rates higher than what is found in other parts of the gastrointestinal tract, and the protozoa are found in the liver and small intestine. The nematodes are also found in various parts of the gastrointestinal tract, including both of the small intestine and the large intestine and esophagus. The species Haemophysalis sp. and Oxylipeurus sp. and helminths Raillietina cesticillus, Raillietina tetragona, Choanotania infundibulum, Hymenolepis sp. Ascardia gallinarum, Capillaria sp.Heterakis gallinarum. and Eimeria sp. were the first recording in turkeys in Iraq. Also the current study showed a many of gross and microspcopic pathological changes caused by lice in infected birds, such as lethargy, laziness and sagging wings and nervous tension and break the feathers and hair loss, as well as change the color of the skin also appeared many of the macroscopic and microscopic changes in the gastrointestinal tract as a result of, intestinal worm included changes macroscopic obstruction and hemorrhagic bruises and blood infiltration The microscopic changes have included the infiltration of inflammatory cells Nicrosis and abstraction of cells lining the of these channels and the proliferation of Kubffer cells and expansion in sinosoids and atrophy of the villi tissue.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: هادي مدلول حمزة الميالي
اسم المؤلف: هند عبد الزهرة عبد الكاظم الشباني

التاثيرات السمية لجسيمات الفضة النانوية على بعض الاعضاء لاناث طائر السمان Coturnix coturnix : دراسة نسجية وكيموحيوية Toxic Effects of Nanosilver Particles On Some Organs of Female Quail (Coturnix Coturnix); Histological And Biochemical Study

المستخلص: تضمنت هذه الدراسة التعرف على تاثير جسيمات الفضة النانوية في نسجية كل من الكبد والطحال والكلى فضلا عن تاثيرها في وظائف الكبد والكلى من خلال قياس بعض انزيمات الكبد المتضمنة (AST & ALT) وقياس مستوى اليوريا والكرياتنين (Urea & (Creatinine, فضلا عن تاثيرها في | This study was included to identify the effect of silver nanoparticles on histology of the liver, spleen and kidney, as well as it's effect on functions of the liver and kidneys through the measurementof some liver enzymes included (AST and ALT) and measurement of urea and creatinine levels, as well as the effect of silver nanoparticles in the weight of studied samples, numbers and sizes of the eggs were produced through the exposure periods.(45) quail (females) were collected from agricultured research center in Abu - Ghraib, divided into (4) groups including : T1 (12 quails were exposed to 4ppm), T2 (12 quails were exposed to 8ppm) and T3 (12 quails were exposed to 12 ppm) of nanosilver particles solution for (60) days.As well as control group T4(9 birds were exposed to distilled water).After the end of the exposure period samples were sacrificed by discapitation of the neck, and blood was collected for the purpose of serum separation which required for biochemical study, samples were dissected and fixated with Bouin's fluid, histological slides were prepared and stained with Eosin - Hematoxylin.Biochemical study were showed significant decrease in the level of liver's enzymes (AST & ALT), urea and creatinine. This decrease was elevated when the concentration was increased, as well as significant decrease in each of sizes and numbers of the eggs were produced through exposure periods and non - significant decrease in the body weight.Exposed groups with silver nanoparticles showed histopathological alterations for each of liver, spleen and kidney, in liver included congestion of blood vessels, infiltration of inflammatory cells, exudate, appearing of concilman bodies, fatty degeneration in most of hepatocytes which progressed to fatty changes in most of samples were exposed with 12ppm, Hyperplasia for each of blood vessel walls and capsule, atrophy of hepatocytes, and appearing of giant cells, while kidney sufferd from hydropic degeneration in most of renal tubules hemorrhage for each of cortex, medulla and glomerulus, hyaline degeneration of tubules, amyloid precipitation for each of glomerulus and blood vessels, sloughing of epithelial cells of renal tubules from basement membrane, hypertrophy of renal tubules with hyperplasia for cells of tubules and atrophy of renal tubules.Spleen tissue showed decreased in sheets of white pulp and lymphocyte numbers red pulp cells. Severity of these effects varied depending on individual differences between samples and concentration of silver nanoparticles, which increase in samples were exposed with highest concentration.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: غزوة درويش النقيب
اسم المؤلف: عبير ساجد عبد علي

استعمال التقانة الجزيئية في تشخيص التباين الوراثي في تراكيب وراثية من الحنطة المتحملة للملوحة Using Molecular Biology In Identification of Genetic Variation In Wheat/Genotypes For Salt Tolerance

المستخلص: This study is made in bio - technology Research Center/Al - Nahrain University 2012 - 2013 for heredity genetic variation by using RAPD and ISSR method between tow genotypes elected to status salinity tolerance 2H and N5 and local varieties Iraq and Latifia sensitive to salinity.Tow experiments are made, first for measure the percentage of germination under conditions of salinity, being of planted the seeds of genotypes and local varieties studied in three replicates and three salt levels 0, 16, 12 ds.m - 1 by 10 seeds in each experimental unit, and after 10 - 15 days of agriculture was to estimate the percentage of germination and the second experiment to study the genetic variation between varieties tolerant and sensitive to salinity, where its seeds are planted soil saline by tow salt concentrations 0, 20 ds/m and after 20 - 25 days of germination, took samples of the leaves of plants to extract the DNA for study genetic variation using RAPD - PCR technique between varieties tolerant and sensitive to salinity.The results of the percentage of germination of the existence of significant differences between genotypes salt - tolerant 2H and N5 and cultivars comparing Iraq and Latifia sensitive to salinity, gave genotype N5 highest percentage of germination was 71%, and gave the genotype 2H germination percentage of 62% in the salt level of salt 16 ds/m, While giving classes comparison Iraq and Latifia less germination percentage reached 16.5% and 25% at the same level of salt, also gave genotypes 2H and N5 germination percentage of 66% and 75%, respectively, while giving classes comparison Iraq and Latifia germination percentage of 50% for both cultivars in the Second level of salt, It is clear from these results that the genotypes N5 and 2H elected from breeding programs and improvement are the most salt - tolerant varieties of local Iraq and Latifia sensitive to salinity at the stage of germination and which is the stage mostsensitive to salinity of the stages of growth of other, particularly in the third level of salt 16 ds/m.The results show that the interaction of RAPD - PCR using 7 primers and there are differences between genotypes N5 and 2H and local varieties Iraq and Latifia and varied this primers in terms of the number of bands and its location was the initiator of OPC - 12 is the best among the primers being of manages to show the discriminative power of through its production bands with a molecular weight 100bp in genotypes N5 and 2H under conditions of salinity just did not show this bands in the local varieties Iraq and Latifia and under the same conditions of salinity, This indicates that this band represents a source of difference between genotypes and local varieties in the degree of salinity tolerance because this band does not appear in all genotypes and cultivars studied under non - saline conditions, it may return this deals band in genotypes and N5 2H under conditions of salinity to show Jenny expression by some of the salt - tolerant gene, which leads to the appearance of this genetic variation in salinity tolerance status.Has also been used 15 primers to determine the genetic variation technique ISSR - PCR between genotypes N5 and 2H and local varieties Iraq and Latifia sensitive to salinity, appeared a number of bands general, however, these primers cannot show any special bands, and thus these primers failed to show this genetic variation between species studied.We conclude by the results that the genotypes 2H and N5 were the most tolerant to salinity at the stage of germination and who have shown a special bands under conditions of high salinity, while the local varieties are sensitive to salinity not appeared in this band under the same conditions and by this difference in the appearance of this package can We concludethat there is a genetic variation between these genotypes and local varieties this may be due to their differences in the degree of salinity disclaim.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: وسام مالك داود ابراهيم اسماعيل المشهداني
اسم المؤلف: غفران علي حسين العبيدي

تاثير بعض عوامل المكافحة الحيوية في بعض الجوانب الحياتية للذبابة المنزلية Musca domestica L. (Diptera : Muscidae) Effect of Some Biological Control Agents On Some The Biological Aspects of Musca Domestica (Diptera : Muscidae)

المستخلص: تضمنت الدراسة الحالية تقويم كفاءة بعض طرائق السيطرة الحيوية باستعمال الفطر Sorokin Metschnikoff)) Metarhizium anisopliae والبكترياBacillus thuringiensis var.israelensis ومنظم النمو Neporex Cyromazine) ) في المكافحة الحيوية للذبابة المنزلية Musca domestica | The present study aimed to evaluation of efficacy of some methods Metarhizium anisopliae Sorokin) Metschnikoff) Bacillus thuringiensis var.israelensis and Neporex) Cyromazine) from be used as biocontrol agents against (Diptera : Muscidae)Musca domestica L. The results showed the following : 1. Different concentration of Fungal suspension have been affected the life stages of Musca domestica. The mortality rate of eggs were (18.66%) at the concentration of 2 × 103 spore /ml, and increased to (36.66%) at the concentration of 2 × 106 spore /ml. The first larvae instars have showed highest mortality rate reached (83.33%) when treated with 2 × 106 spore/ml, while (56.66%) of them were dead at concentration of 2×103 spore/ml. (36.66%) of pupae of Musca domestica were dead when exposed to 2 × 106 spore /ml. The treatment with highst concentration caused high adult mortality, i.e. (96.66%) and (93.33%) for adult males and females of the Musca domestica respectively after 168 hours, while (70%, 73.33%) when treated with 2×103 spore /ml. 2. The secondary metabolities of fungi when applied, all first larvae instars of Musca domestica were dead and (96.66%) of the when tretead with concentration 100%, while (66.66%) and (63.33%) for the same larval instars at concentration 25%. 3. The result showed the different concentration of bacterial suspension affected the mortality rate of larvae of Musca domestica.The first larva instars have showed highst mortality rate which reached (76.66%) when treated with 2 × 105 spore /ml while (50%) of them where dead at concentration 2 × 102 spore /ml after 72 hours from treatment. 4. The effect of secondary metabolites of bacteria when applied, All first larva instar of Musca domestica were dead and (90.00%) when treated with concentration 100% and decreased to (73.33%) after 72 hours from treatment , the mortality rate of adult was (100%) at concentration 100% and decreased to (63.33%) after 72 hours from treatment. 5. The concentration of insect growth regulator neporex have effected , The highst mortality rate eggs was (37.00%), at the concentration 100 part per million, while (19.33%) at the concentration 25 part per million, , the first larval instars mortality at the concentration 100 part per million, was (96.66%) at the concentration 100 part per million while (66.66%) at the concentration 25 part per million.the mortality rate of pupa was (73.33%) while (30%) at the concentration 25 part per million after 72 hours from treatment
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: محمد رضا عنون الحسناوي
اسم المؤلف: علي مرتضى كاظم الياسري

اثر التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراء (mays L. Zea) تحت نظام الري بالتنقيط Effect of Foliar Nutrition of Potassium And Chelated Iron In Growth And Yield of Corn (Zea Mays L.) Under Drip Irrigation System

المستخلص: نفذت تجربة حقلية في محطة ابحاث محاصيل الغالبية /محافظة ديالى في الموسم الخريفي 2013في تربة ذات نسجة طينية غرينية بهدف معرفة تاثير التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراءL.) Zea mays (صنف بحوث 106 تحت نظام الري بالتنقيط بتصمي | A field experiment was conducted during autumn season 2013 in silty clay soil at Field Crops Researchs Station in AL - Ghalbiea/ Diyala Governorate.The aim of this experiment to study effect of foliar nutrition of potassium and chelated iron in growth and yield of maize(Zea mays L.) Buhooth 106 under drip irrigation system.Random Complete Block Design (RCBD) was used in this study with three replications. Spraying four concentrations of potassium(0, 1000, 2000, 3000) mgK. L - ¹ as potassium sulfate K%41.5 and four concentrations of chelated Iron EDTA(Fe%13)(0, 50, 100, 200)mg Fe.L - ¹. The results showed that the suitable) K (foliar nutrition treatment was 3000mgK.L - ¹ which caused significant differences in plant height, number of leaves, stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grain per ear, weight of 500grains, grain yield, chlorophyll, concentration of K and concentration of Fe as compared with control, it in order to195.87cm, 15.51 leaf, 27.32mm, 59.11dm2, 6017g.m2, 20.158cm, 17.025row, 516.75grain, 143.75g, 9.507t.ha - 1, 51.34 SPAD units, %3.003, 145.36mg.Kg - 1.Using Fe - foliar nutrition treatment was 50mgFe.L - ¹ caused significant differences in plant height and number of leaves it 193.76cm and 15.54 leaf، and 100mgFe.L - ¹ caused significant differences in stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grains per ear, weight of 500grains, grain yield, chlorophyll and concentration of K as compared with control it in order of 27.76mm, 60.41dm2 , 6004g.m2, 20.95cm, 17.15 row, 505.33 grain, 143.00g , 9.646 t.ha - 1, 50.08 SPAD unit, 2.9284%.While 200mgFe.l - ¹ caused low of all traits parameters except concentration of Fe in leaves was 188.18 mg.Kg - 1.The interaction between potassium and iron K3000 * Fe100 of significant effect in more traits parameters compared with control treatment and concentration of potassium decreased high level of iron(200mgFe.L - ¹) of monitor form
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: نجم عبد الله جمعة الزبيدي
اسم المؤلف: ايمن احمد عبد الكريم العباسي

تعيين بعض الظروف الزرعية لانتاج انزيم السليليز والكحول الاثيلي من بعض انواع البكتريا المعزولة من التربة Determination of Some Cultural Conditions For Cellulase And Ethanol Production By Bacteria Isolated From Soil

المستخلص: The study included isolate and diagnose of some types of anaerobic and aerobic bacteria capable of decomposing and fermentation of cellulose. Isolation and diagnose the bacteria Clostridium phytofermentous from 10 agricultural soil samples, was 50 % of the total 50 colony, while isolation of Escherichia coli from 15 waste water samples which represented 40% of the total 75 colony and isolated Pseudomonas aeruginosa from 15 normal soil samples, and was 53% of the total 75 colony. production of cellulase enzymes was evaluated by using absorbance method. Higher activity obtained via Clostridium phytofermentous with maximum value of 42.8 IU / mL, while Pseudomonas aeruginosa gives 12.5 IU / mL and Escherichia coli gives 29.1 IU / mL.. Optimum temperature was 35 C and pH 7, for production cellulose enzyme The concentration of ethanol Alcohol was measured by using Titration method. Maximum concentration of ethanol alcohol produced by Escherichia coli, which reached 8.28 g/liter, while Pseudomonas aeruginosa was 7.86 g/liter, and Clostridium phytofermentous reached 6.62 g/L.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عدنان نعمة عبد الرضا العزاوي انيس عبد الله كاظم
اسم المؤلف: عبد الستار عبد الجبار ابراهيم

دراسة بيئية للطحالب الملتصقة على بعض النباتات المائية في مياه مبزل الفرات الشرقي (الحفار) / الديوانية - العراق An Ecological Study of Epiphytic Algae On Some Aquatic Plants In East Euphrates Drainage Water /(Al - Haffar) Al - Diwaniya/Iraq

المستخلص: تناولت الدراسة الحالية الخصائص البيئية المؤثرة على الطحالب الملتصقة على النباتات المائية في مبزل الفرات الشرقي (الحفار) في الديوانية - العراق, اذ شملت الدراسة قياس بعض الخصائص الفيزيائية والكيميائية لمياه المبزل والخصائص الاحيائية التي تضمنت دراسة كمية ون | The present study tackles ecological characteristics which is effected of Epiphytic algae in east Euphrates drainage (AL - Haffar) in AL - Diwaniya, This study included the measurement some of physical and chemical characteristics of drainage water, and biological characteristics which includes studying quality and quantity of Epiphytic, Three aquatic plants Ceratophyllum demersum, Phragmits australis, and Imperata cylindrical had been selected, Samples collected monthly during The period from November 2012 to October 2013.Three location have been chosen, the first is near AL - Abtan village, Second is after the first location five kilometers, while the third location is after the Second about seven kilometers. The study shows that the temperature of the air is about (7 - 39c) and water temperature is approximately (10 - 33c), while light penetration is about (10.5 - 63cm) the flow speed is (0.25 - 0.68 M/Sec), Water turbidity is (40.02 - 63.03 NTU), while Electrical conductivity is approximately about (3014 - 3964 Sc/cm). The results of the study shows that the water drainage Oligohaline, the salinity values ranged from (1.92 - 2.53‰), the total (T.D.S) is about (1741 - 2226 mg/l), while the T.S.S. is about (41 - 67 mg/l). The result of this study shows that the pH is limited Through all the period of the study (6.98 - 8.18), The drainage water get good airing dissolved oxygen values are(6.71 - 8.14 mg/l), biological oxygen demand are (2.05 - 5.23mg/l) and carbon dioxide values are about (81.50 - 119.57mg/l).The results of this study also show about that drain water is alkaline and belonged to bicarbonate ions, total alkaline is (140.28 - (189.27 mg/l) It is discovered that the drain water is too hard, hardness values are (512.15 - 612.45mg/l), while Calcium concentrations are (128.36 - 173.40mg/l) and Magnesium concentrations are (30.31 - 50.09 mg/l). Plant nutrients, N concentrations are about (0. 98 - 3.99 µg/l) of Nitrit, Nitrate concentrations are (216 - 415 µg/l) and Phosphate concentrations are about (0.9 - 2.92 µg/l), and The percentage of total organic carbon in the residues is(0.59 - 0. 17) %. The total number of epiphytic algae types which are diagnosed during study period reaches to 320 species belongs to 86 genera within 7 classes They are Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae Xanthophyceae, Dinophycea eand Rhodophyceae, Bacillariophyceae obtaints the dominion which reach to (183) species of Bacillariophyceae. It makes rate (57.19%).Than Chlorophyceae came concerning types number 68 species in rate (21.25%) , Following by Cyanophyceae 53 species in rate 16.56%. Euglenophyceae are 10 species in rate (3.13%).Xanthophyceae is represented in two species belong to2 genera.It makes proportion (0.63%) , Dinophyceae which represented in 3 species belonged to 3 genera in rate (0.94 %) and Rhodophyceae represented in one species and one genera in rate (0. 3%) of total number of algae.Class of the Bacillariophyceae, during study period were Navicula وNitzschia وCymbella وSynedra وAchnanthes, genera of Oscillatoria and genera of, Scenedesmus manifest that highest number of available types during the period of study.It has note that there are numbers of the monthly and located changes in total number of epiphytic algae on host plants.was varied in months and locations due to the host plants. Ceratophyllum demersum marks the Highest total number of epiphytic algae 172.89× 104 individual/ gm of wet weight in third location during April. Imperata cylindrica marks the lowest total number of epiphytic algae 3.51×104 individual/ gm of wet weight in scecond location during January, The total number of epiphytic on host plants ranges between (175.39 - 668.6) ×104 individual/ gm of wet weigh.The total number of main epiphytic algae classes is varied in total number of epiphytic algae.Bacillariophyceae diatoms composed 81.02%, in all locations during the study period, followed by Cyanophyceae composed 9.7%, while Chlorophyceae composed 8.6%.Euglenophyceae, Chrysophyceae, Dinophyceae and Rhodophyceae, were not important concerning their number. Statistical analysis results show there are significant differences between locations and months of study in relation to physical, chemical and biological features. Also there are connected correlations positive and negative between epiphytic algae and physical, chemical and biological features that measured during the study period of probability level of P<0.05.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات - الاحياء المائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: فؤاد منحر علكم
اسم المؤلف: زهرة كليب مهدي الخزعلي

دراسة مظهرية وتشريحية مقارنة للجنسين Melilotus Mill. وScorpiurus L. (Leguminosae) في العراق A Comparative Morphological And Anatomical Study of Melilotus Mill. And Scorpiurus L. Genera (Leguminosae) In Iraq

المستخلص: اجري البحث الحالي في العام الدراسي (2013 - 2014), اذ تناول دراسة مظهرية وتشريحية مقارنة للجنسين Melilotus Mill. وScorpiurus L. في العراق, والتي شملت اربعة انواع للجنس الاول وهي M. alba Desr. وM. indica L. وM. messanensis L. وM. officinalis L. ونوع واحد ذ | The current research was conducted in the academic year (2013 - 2014), it a comparative morphological and anatomical study of Melilotus Mill. and Scorpiurus L. genera in Iraq, which included four species of the first genus M. alba Desr., M. indica L., M. messanensis L. and M. officinalis L., and one species is two variety to second genus (S. muricatus var. muricatus L. and S. muricatus var. subvillosus L.).Morphological characteristics studied and carried it comparison, starting from the root and the end of fruit and the seed for all species of genera and adopted the most attested qualities and importance in the diagnosis and isolation of those species, as well as prepared the tables, paintings and painted illustrations that serve this purpose. The stem length importance in isolating S. muricatus var. muricatus for M. alba, and through follow - up field for two types of M. indica and M. messanensis observed that the young’s stem contain the trichomes which disappear in the amounting stem for them. It can be used peduncle to isolate the genera from each other in non - interference between them. the flag leaf height character can be to isolate the M. indica from the other species of Melilotus genus has been a summit flag leaf contrast and a clear contrast between the species under study were obtuse in variety of S. muricatus species and retuse or emarginate in M. officinalis while it was emarginate in the other species. The colors of corolla, forms of petals, forms of stipules and size it, shape of the leaves, margins, size and shape of pod as well as color seeds, surface and volume of taxonomic importance of clear and significant in the diagnosis and isolation of species.Studied the anatomical characteristics of each stems, petioles, leaves and venation to species of genera under study, it was stem and transvers section forefront of the anatomical study from the rest of the other parts.The cross sections of the leaves blades showed taxonomic importance in terms of blade thickness and the number of rows to intrafascicular bundle and the number of bundles in each row and thick it possible could be diagnosis of genera species, normal epidermis cells and stomatal type to leaves exchange also studied increased the important qualities in diagnosis, also studied the anatomical characteristics of petioles, and the transvers section had taxonomic importance could be through the divided of species on four groups depending on what it had helped to diagnosed and isolate of species gender. It was a taxonomic key to the separation of the genera species under study and described four species of Melilotus and one species with two variety of Scorpiurus accurate and detailed description.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: سهيلة حسين باجي اللامي
اسم المؤلف: وسام عيدان جبر الشمري

دراسة تاثير الذيفان حال الدم الفا المستخلص من بكتريا Echerichia.coli في فعالية واستجابة الخلايا المناعية Study The Effect of The Alpha Hemolysin Toxin Extracted From Echerichia Coli In The Activity And Response of Immune Cells

المستخلص: تضمنت الدراسة الحالية محورين الاول الحصول على عزلات من بكتريا E.coli من المرضى المصابين بخمج السبيل البولي اذ تم الحصول على اربعة عزلات من بكتريا E.coli من مختبر الصحة العامة في كربلاء، وعزلة من بكتريا E.coli من براز شخص سليم من مستشفى الاطفال في كربلاء و| The isolation of E.coli bacteria from patients with urinary tract infection, Four isolates were obtained from the public health laboratory in Karbala, and isolation of E.coli bacteria from the feces from healthy person and S.aureus from the public health laboratory, on the basis of microscopic diagnosis and biochemical test isolates were confirmed diagnosis.This axis also included the investigation of the ability of four UTIs isolates to produce the toxin qualitatively on blood agar and measuring the diameters of hemolysis and quantitatively by measuring the amount of hemoglobin released from the RBCs.The toxin (hemolysin) was extracted from the most efficient isolate, after the cultivation in the chemically defined medum (CDM) The highest dilution gave hemolytic activity on blood cells 1/32 (320 units / ml), but after sedimentation, dialysis and freeze - drying found that (1 / 64) caused hemolysis of 50% of the blood cells compared to the standard curve.The results of this study, indicated that the role of the toxin (Hemolysin) in the pathogenecity and virulence of E.coli, lowered the lethal dose (LD50) of the bacteria which decreased from (107 × 3.16 cells / mL) to (106 × 2.34 cells / mL).The second axis included the study of the immune responce included : Studying the effect of the toxin on the immune cells isolated from the peritoneum of the mice through its effect on viability of these cells, using the concentrations of the toxin that showed the hemolytic activities (100, 90.70, 50.25) %, as well as determine the effect of the toxin on the phagocytosis S.aureus process in different periods The study found that the toxin effect on the immune cells depends on the concentration used.The low concentrations of the toxin, which showed hemolytic activity (25%) did not affect the viability of immune cells and their ability to phagocytosis where the results of the statistical analysis showed no significant difference (0.05 <p) in comparison with control, while high concentrations of the toxin showed effects on the viability of immune cells and on phagocytosis where statistical analysis significant difference (0.05> p) compared to control treatment, and the results showed that the effect of the toxin on the phagocytosis depended on the concentration and time, where phagocytic Index decrease with dose and time.It was estimated the level of cytokins (IL - 1B, IL - 2, IL - 6, TNF) in plasma were estimated for mice injected with sub lethal dose 50 (106) of the non hemolytic E.coli isolated from stool and another group which injected with LD50 of the toxin (25%) and a third group were injected with a mixture of bacteria the toxin in addition to the control group injected with normal saline, The level of cytokins during different periods of time (6, 12, 24, 48, 72) hour were estimated, where it was found that the sub LD50 of the non hemolytic E.coli had no effect on the level of TNF while sub LD50 of the toxin had no significant effect as well as the doses of the mixture in comparison with control treatment while the other cytokins, Results showed little rise in the level of IL - 1? in mice injected with non hemolytic bacteria and when the injection with sub LD50 as well as two doses mixture was large, as well as the case for IL - 2 and the IL - 6, but in different proportions, from this we conclude that the toxin stimulates the liberation of the IL - 1 and IL - 2 and the IL - 6 from immune cells, but does not affect the TNF.
الصفحات الاولى:
مكان الجامعة: كربلاء
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: علوم الحياة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: ذكرى عدنان جواد المسلماوي
اسم المؤلف: وقار عدنان حمدان الكبيسي

دراسة مناعية لمستضد متعدد السكريد للفطر Aspergillus niger في الارنب Immunological Study of Aspergillus Niger Polysaccharide Antigen In Rabbit

المستخلص: جمعت خلال هذه الدراسة 50 عينة تمثلت ب 25 عينة تربة و25 عينة قشع من اشخاص مصابين بالتدرن الرئوي والوافدين لمركز التدرن والامراض الصدرية في محافظة بابل خلال المدة المحصورة للفترة من تشرين اول 2013 ولغاية شباط 2014 تم خلالها عزل الفطر Aspergillus niger وبنس | fifty specimens were collected, 25 samples were collected from soil while the other 25 sample were collected from sputum of pateints with Tuberculosis, who admitted to thoracic diseases center in Babylon province in period October 2013 to Febreury 2014. Aspergillus niger Has been Isolated from these sample with 56% appearance in soil & 48% in sptum of TB pateints.these isolated were examined micro and macroscopically. purified Fungi was culture on potato dextrose broth, after incubation period polysaccharide antigen of A.niger cell wall was prepared for isolated from soil and TB patients suptum. Molish, protein and glucose test to provide that these antigen are polysaccharide, then immunuization of local rabbit of Orcyctalagus conninculus age 3 - 5 months & 1 - 1.5 Kg for males only (3 animals for each antigen) After the immunization period (one month) some of local and systemic immune response parameters (cellular and humoral) to test animals were studied and compared with control animals which immunized with normal saline. The results of study showed that the polysaccharide antigen isolated from these two strains lead to stimulate systemic humaral immune response which had been studied by using direct agglutination test for appendix immunoglobulin passive haemagglutination test (both were used to determined antibody titer), radialimmunodiffusion test in order to determine level of concentration of immunoglobulins (IgG, IgM) and complement part (C3, C4). where The results of direct agglutination titer of animals immunized with polysaccharide antigen 128 and 1 for control animals and heamagglutination tests titer of animals immunized with polysaccharide antigen serum (2560). These results were higher compared with control group titer (10) and this is also true for immunoglobulin and concentration of IgG, IgM for immunized animals compared to control one. In addition to that The concentration of C3 and C4 had been increased in immunized animals compared to the control. cellular immunological response had been measured by using rabbit Neutrophil Elastase Kit. The eresult show an increase in the level of phagocytosis in animals immunoized with polysaccharide antigen isolated from A.niger cell wall of TB pateints 0.37±0.03 pg/dl while decreased in animals immunoized with polysaccharide of soil A.niger 0.72294±0.25548pg/dl compared with control animals 0.6±0.16386 pg/dl. on the other hand the humaral adaptive immune response had been identified by measuring cytokine IL - 2, IL - 10 in serum rabbit, the level of IL - 2 in rabbit immunoized with polysaccharide antigen soure TB patients and soil is 5.4129±1.08979 and 4.2641±0.62997 pg/dl respectively compared with control animals 3.9327±0.55587 pg/dl. The level of IL - 10 had been dropped for immunized animals with A.niger of TB pateints and from soil 4.8762±1.15104 and 8.2632±4.31933 pg/dl while the control animals having the highest level 10.2576±5.23356 pg/dl among the groups. IgE concentration measured significant difference at p>0.05 in immunized animals of A.niger in both TB pateints and soil 1.1701±0.05811 and 1.0770±0.19355 ml/Iu compared with control where IgE0.8723±0.0849ml/Iu. Miotic index of bone morrow immunized and control animals show mean difference is signification at p>0.05.where the result demonstrate that the polysaccharide antigen fissed the bone marro cells compared to controlled one. In order to identify the viability of antigen in protection. challenge dose test made by injection animals with 108 spore/ml of A.niger conidia which prove their protection in immunized animals while the control animals undergo many morphological and behavioral changes after 5 - 7 days of their injection.I addition that the size of lung and spleen increased when compared with immunized one. The histological culture of lung and spleen of challenge test animals in order to identify the ability and speed of their body to evadicate and prevent infection, display that A.niger coloni appeared for control animals (6 rabbit) while disappeared in culture of immunized one. Skin test also performed to experimental animals which indicate that polysaccharide antigen of A.niger interact with hypersensitivity test of rabbit, where the level of late hypersenetivity increased clearly with propability of p>0.05 with the highest level after 24 hours of beginning test compared to controlled one.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: نداء شهاب حمد فريال جميل عبد
اسم المؤلف: غدير كاظم عبود الخفاجي

دراسة بيئية وحياتية للمجتمع السمكي في نهر الفرات/ وسط العراق Ecological and Biological Study of fish Community in Euphrates River / Middle of Iraq

المستخلص: اجريت الدراسة الحالية في نهر الفرات عند سدة الهندية خلال المدة من تشرين الاول 2012 لغاية ايلول 2013 لغرض دراسة بيئة وحياتية المجتمع السمكي، وتم اختيار موقعين، يقع الموقع الاول في شمال سدة الهندية، ويقع الموقع الثاني في جنوب سدة الهندية، وتضمنت الدراسة تاث | The Present study was carried out on the Euphrates River during the period from October 2012 to September 2013 to study ecology and biology of fish community in the river, two study sites were chosen the first one was in the north and the second one was in the south of Al - Hindiya dam.The study was included the effects of some physical, chemical factors of water and Al - Hindiya dam on ecological and biological of fish population as well as measurement of the concentrations of some heavy metals (Pb, Cd, Mn, Zn) in both dissolved and particulate phases of water and in the tissue of three types of freshwater fish that was high dominance in three age groups. Study of the number of species, individuals, Total of weight of individual. Some ecological indices were study as index of Relative abundance, Species Richness index and Shanon wiener index for determining the evaluate of biodiversity of fish. Also Jaccared index of similarity was used to explain the similarity between sites of study and among months. Constant index was used to determine the constancy of taxa. Biological study of this of three fish that have high dominance from the side of food, age, growth, sex ratio, gonado somatic index and length - weight relationships. Air temperature and water temperature were ranged in two study sites between (15 - 43.8) C? and (10 - 29) C? respectively, pH values were between (7.6 - 8.6), Water current velocity values were between (0.14 - 0.72) m/sec, light penetration values were between (27.3 - 193.3) cm, the value of electrical conductivity were between (822 - 1323) ? sem/cm , Total dissolved solid values were between (663.3 - 932) mg/L, while The total suspended solid were between (0.009 - 0.23) mg/L and the values of dissolved oxygen were ranged between (6.13 - 10.33) mg/L.The biological oxygen demand were recorded values that range between (0.68 - 4.3) mg/L. . The water of Euphrates river were considered slight alkaline according to the results of present study and ranged between (33.33 - 164.3) mg CaCO3/L and the water were very hard according to high values record for total hardness (391.6 - 886.6) mg CaCO3 /L, while the values of calcium and magnesium hardness were ranged between (86.1 - 188.4), (19.8 - 138) mg CaCO3/L respectively.The values of phosphate were ranged between (0.51 - 13.12) ?g/L and Nitrate values were ranged between (6.23 - 70.17) ?g/L, while the values of nitrite were ranged between (0.17 - 4.98) ?g/L. in especially, the effects of Al - Hindiya dam, the results were showed that numbers of species before and after of Al - Hindiya dam were ranged between (23 - 17) species respectively. the results were showed positive and negative correlation between physical, chemical properties of water and Heavy metals with number of species, individuals, weights and Biodiversity indices. This study showed that the mean concentrations of the heavy metals (Pb, Cd, Mn, Zn) of the dissolved phase in water were (2.14, 1.61, 3.10, 7.63) ?g/L respectively and in the particulate phase were (33.99 112.87، 84.21، 21.12،) ?g/g dry weight respectively, the results of present study were showed that the concentration of heavy metals in water in dissolved and particulate phases within local and world limitations. The heavy metals in muscular tissue for three groups of this fish were ordered in as following : Zn >Mn > Pb > Cd. Twenty - three fish species were collected, (17) of them belonged to Cyprinidae family and two species belonged Bagridae family while only one species represented each family of Mugilidae, Siluridae, Mastacembelidae, Cichlidae, the number of species were ranged during the period of study between (5 - 18), The total number of individuals was 2401 individual and the number of individual were ranged monthly between (129 - 276) individual, while the total weight of individuals were 232868 gm during the period of study and The weight of individuals were ranged monthly between (13072 - 29598) gm. Carassius auratus wae recorded with the highest percentage in number and weight as 35.2% and 27.5% respectively. while A.vorax was after C.auratus in total number 13.6%, but it was after C.auratus and C.carpio in total weight 16.9% of the total individual weight. The values of species richness index were ranged between (0.79 - 3.23) and numerical of shanon wiener index values (Hn) were ranged between (1.26 - 2.39), while the weight of shanon wiener index value (Hb) were ranged between (1.21 - 2.35). the numerical and weight of eveness index (Jn) were ranged between (0.65 - 0.86), (0.68 - 0.89) respectively during the period of study. Jaccared similarity index were ranged between (23% - 92%). And the study was revealed that the most species recorded during the period of study were constant species according to constancy index. C. carpio fish were Omniovorous and It's feeding activity was ranged between (70% - 91.42%) and feeding intensity was (6.9 - 12.17) degree/fish.while C. auratus were detritious, It's feeding activity was ranged between (56.75% - 94.28%) and feeding intensity was (6.91 - 12.87) degree/fish. A. vorax were carnivorous and It's feeding activity values were ranged between (69.2% - 90.32%), while feeding intensity values were ranged between (6.85 - 13.03) degree/fish. The results was showed the values of age and length for C. carpio that ranged between (1 - 5) years, (9.7 - 40.2) cm , and C. auratus were ranged between (1 - 6) years, (8.3 - 28) cm, while A.vorax were ranged between (1 - 7) years, (10 - 62) cm respectively. Sex ratio and gonado - somatic index (GSI) varied between the three species, sex ratio of C.carpio (male : female) was 1 : 3.1. (GSI) for male was ranged between (2.21 - 12.02) and for female between (3.36 - 19.36).The sex ratio of C. auratus male : female was 1 : 1.9, GSI values for male was ranged between (0.89 - 8.53) and for females between (1.23 - 61.37). A.vorax sex ratio male : female 1 : 1.5, GSI value for male and female were ranged between (0.36 - 2.84) and (0.24 - 5.83) respectively.Variation of values of slope factor (b) were calculated from length - weight relationships for three species of fish, the values of (b) factor for C.carpio, C.auratus and A. vorax (3.08, 2.97, 2.92) respectively during the period of study
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة - التلوث البيئي
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ميسون مهدي صالح الطائي مؤيد جاسم ياس العماري
اسم المؤلف: ماهر محمد خضيري

دراسة تصنيفية لعويلة الخنافس البرغوثية Alticinaeمن عائلة Chrysomelidae ورتبة غمدية الاجنحة Coleoptera في بعض محافظات العراق Taxonomic Study On The Flea Beetles (Coleoptera : Chrysomelidae : Alticinae) In Some Provinces of Iraq

المستخلص: تناول البحث لهذه الرسالة دراسة تصنيفية لعويلة الخنافس البرغوثية Alticinae في بعض محافظات العراق، علما ان هذه العويلة لم تدرس تصنيفيا في العراق.اختير النموذج Longitarsus ballotae Marsham. لتجسيد صفات العويلة. ولقد درست الاجزاء الرئيسة للجسم وهي الراس والص | The Subject of thesis is a taxonomic study of Subfamily Alticinae in some provinces of Iraq. Knowing that this Subfamily has not been studied taxonomically in Iraq.Was chosen as the model for Longitarsus ballotae Marsham. To embody the gualities of the Subfamily. The detailed study of the head, thorax, abdomen and their appendages was made to evaluate the reliability of the external characters in classifying the species of the Subfamily.There are eight genera and thirteen species for these Subfamily The species are : - Altica deserticola Weise.Aphthona fuentei Reitter.Aphthona sp. Chaetocnema montenegrina Heikertinger.Chaetocnema persica Baly. Epitrix atropae Foudras.Hermaeophaga ruficollis Lucas.Longitarsus ballotae Marsham.Longitarsus membranaceus Foudras.Longitarsus reichei Allard. Longitarsus succineus Foudras.Phyllotreta nemorum Linnaeus.Podagrica fuscicornis Linnaeus.6 Species Register in Iraq are : - Chaetocnema montenegrina Heikertinger.Epitrix atropae Foudras. Longitarsus succineus Foudras.Longitaarsus membranaceus Foudras.Podagrica fuscicornis Linnaeus.Two species Taxonomic keys were designed and developed to is olate the genera of Subfamily Alticinae, sa wellas the genera mentioned and studied in this thesis for each species.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: علوم الحياة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: سعدون ابراهيم اسماعيل
اسم المؤلف: رغد عـبيد خضير

عزل وتشخيص بكتريا Streptococcus spp المسببة لالتهاب اللوزتين من الاطفال وتاثير بعض عزلات بكتريا حامض اللبنيك عليها Isolation And Identification of Streptococcus Spp Caused Tonsillitis From Children And The Effect of Some Lactic Acid Bacteria (Lab) Strains On It

المستخلص: بحثت الدراسة جمع 100 مسحة من اطفال مصابين بالتهاب اللوزتين الحاد والمزمن ممن راجعوا استشارية الانف والاذن والحنجرة في مستشفى اليرموك التعليمي للمدة مابين كانون الاول للعام 2013 ولغاية اذار للعام 2014. وكانت نتيجة الزرع المختبري موجبة في 67 مسحة منها وبالا | In this study 100 swabs were collected from infected children with acute and chronic tonsillitis who attended at Al - Yarmook Teaching Hospital (Eear Nose Tonsils consultation clinic) from December 2013 until March 2014. The result of laboratory culture were positive in 67 samples. Depending on their cultural, morphological and biochemical characterization of bacterial isolates : (37.31%) of them were identified as Streptococcus pyogenes bacteria and the diagnosis is confirmed by the use of Remel Rapid STR System. Whereas (34.33%) belong to S.parasanguinis, (11.94%) S.mitis, (11.94%) S.oralis and (4.48%) S.thoraltensis were identification of them are confirmed by using Vitek - 2 System also. As well as Sensitivity test for S.pyogenes cultures for some of the antibiotics done and the results revealed that all cultures were sensitive to tetracycline and chloramphenicol drugs 100%, while it was different in their sensitivity for other drugs. According to age (2 - 13) years revealed the highest percentage of infection for both male and female, however the percentage of acute and chronic tonsillitis was higher in males than females. In this study, during investigation of the antagonist action of lactic acid bacterial isolates against these pathogenic bacteria, results revealed that the method of L.acidophilus discs gave a high inhibition zones with a diameter of 11.3 mm, while L.fermentum discs yielded a high inhibition zones with a diameter of 7 mm 24 hrs following incubation in comparison with well diffusion method by using suspension of L.acidophilus produced a high inhibition zones with a diameter 7.25 mm 48 hrs after incubation. L.fermentum suspension produced a high inhibition zones with 10 mm diameter 24 hrs following incubation. Also the study included measurement of the inhibition activity for bacteriocins produced by L.acidophilus bacteria against the pathogenic bacteria S.pyogenes on nutrient agar by well diffusion method in which results revealed stability of the bacteriocins effects under different PH (4, 5, 6) with percent (100, 60, 50)% respectively for 24 hrs at higher stability and the highest stability of bacteriocins during PH was 4 with percent 100%, while it lost a lot of its activity with acidic PH less than 2 and basal pH more than 8. During the study the effect of Nacl, Kcl and MgSo4 with different concentrations (1 - 5%) with constant inhibitory effect for bacteriocins produced by L.acidophilus against pathogenic S.pyogenes, The result revealed that Nacl had little effect in inhibition zone with 1&2% concentrations. The salt MgSo4 and Kcl showed reduction in the inhibitory activity with 1, 2, 3% concentration, however the higher concentration of salt caused great reduction as 5% concentration led to loss of inhibitory activity for bacteriocins completely.Also this study showed light on the inhibitory effect of probiotics suspensions against the adhesion property of S.pyogenes after treated with L.acidophilus and L.fermentum.where the Result shown that L.acidophilus inhibit the adhesion of S.pyogenes with percent 68.92% either L.fermentum inhibit the adhesion of this pathogenic bacteria to epithelial cell with percent 30.28%. The study is consisted of showed the inhibitory action for suspensions of L.acidophilus and L.fermentum on biofilm formation by S.pyogenes, it was noticed the action of L.acidophilus in the side more than from the action of L.fermentum. During the study of the effect of suspension and filter of L.acidophilus and L.fermentum on the production of Hemolysin enzyme by S.pyogenes, it was noticed that the ability of suspension belong to L.acidophilus and L.fermentum to reduce the diameter of hemolysis zone which was beta type more than the ability of filter L.acidophilus and L.fermentum to reduce of the hemolysis zone which was beta type.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: ندى صباح رزوقي
اسم المؤلف: رند ثائر عبد اللطيف

الخصائص التشريحية والتصنيفية لانواع مختارة من ذوات الفلقتين البرية النامية في محافظة ديالى Anatomical And Taxonomical Attributes of Wild Dicots Selected Spesies In Diyala Pravince

المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لعشرة انواع مختارة من العائلات المختلفة لذوات الفلقتين. النامية في مناطق الصدور والسوامرة وطريق العظيم، التابعة لمحافظة ديالى، والانواع هي : - Centaurea bruguieriana (Dc.)Hand.Mzt. 1.Onopordon acanthium Eig. 2.Ero | Ten wild selected species belong to different Dicots families were anatomically comparative studied. These species grown wildly in Sudoor, Suwamra, and Kallis _Udaim way of Diyala province, as follows : Centaurea bruguieriana (Dc.)Hand.Mzt. 1.Onopordon acanthium Eig. 2.Erodium glaucophyllum (L.) L' Her. 3.Eruca sativa Mill. 4.Matthiola longipetala (Vent.)Dc. 5.Psylliostachys spicata (willd.) Neaski 6.Ranunculus cornutus DC. 7.Rumex cyprius Murb. 8.Scabiosa palaestina L. 9.Verbena officianlis L. 10. The research concentrated on the anatomical comparative characters for the first time in Iraq. These characters regarded as a Diagnostics for these species. Epidermis with its variable cells and stomatal complexes were investigated, so as for venation in leaves, mesophyll and vascular bundles. According to the above characters the species were divided in to groups. This work was determined the distinguish characters of the petiols, midribs of leaves, in addition to the anatomical information of stemes, roots and different kinds of indumentum and glands which were aided to separate these species. The variable anatomical results were important and were assisted the morphological ones. Field photographs for the specimens, schedules were given, and the results were also discussed according to the environments of the Taxa studied.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: علوم الحياة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: علي حسين عيسى الموسوي محمود شاكر رشيد الجبوري
اسم المؤلف: ضفاف خليل سلام البدري

دراسة نسجية مقارنة بين عضلات الصدر والارجل في نوعين من الطيور الطائرة وغير الطائرة في عمرين مختلفين Comparative Histological Study Between Pectoralis And Leg Muscles In Flying And Running/Walking Birds In Two Different Ages

المستخلص: تناولت هذه الدراسة عددا من العضلات الهيكلية في نوعين من الطيور الطائرة (الحمام) وغير الطائرة (الدجاج). العدد الكلي للطيور المستعملة 30 طائرا, 15 من الدجاج و15 من الحمام تمت دراسة بعض الخصائص الفسلجية والنسيجية للالياف العضلية الحمر والبيض والوسطية وحساب | This study deals with a number of skeletal muscles in two types of birds ; flying (pigeon) and running (chicken) types. The total number of the studied birds was 30; 15 were chicken and 15 were pigeons. Some of the physiological and histological characteristics of the muscle fibers (red, white, and intermediate) , the percentage of fibers diameter in the studied muscles, and the change noted in the muscle weight with advancing age had been calculated. The species of the chicken in this study was Gallus domesticus , while the species of the pigeons was Columba livia domestica. The muscles that is used in the study were : pectoralis muscle, femortibialis, triceps, biceps brachii, extensor carpi radialis muscle, and gastrocnemius muscles in two age groups of birds; (3 - 6) months and (6 - 12) months in order to know the changes in the weights, numbers, diameters, and sizes of the muscle fibers that occur with advancing age. The aim of this study is to identify the three types of the muscle fibers their role in the muscle movement and the regions in which there is specific type rather than others. The results had shown that the skeletal muscles in birds consist of heterogenous mixture of the three types of muscle fibers (red, white, and intermediate) , and the white muscle fibers are the predominant type in the pectoralis muscle of both types of birds, and the size of white muscle fibers in the pectoralis muscle in the chickens in the first age group was 73.8%, and in the second age group was 82.9%. While in pigeons, the size of white muscle fibers was 75.5% in the first age group, and 75.4% in the second age group. In addition, the present study deals with the relationship between the muscles weights and the age of the bird in that the muscles show increased weight with advancing age in both types of birds and there were significant differences in muscles weight in both age groups (p?0.05), while the triceps muscle did not show significant difference regarding weight between the two age groups in the same type of birds. The results of the study also show the differences in the diameter of the muscle fibers, that is there was continuous increase in the diameters with advancing age and show significant differences (p?0.05) , but regarding pectoralis muscle there was no significant differences in diameters with advancing age in both types of birds. The results of the study also show that the pectoralis muscle was the largest muscle in the body of the bird, and the ratio of its weight to the total body weight was relatively large.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: هاشم محمد عبد الكريم العلاق
اسم المؤلف: تغريد حامد عبد الامير

تاثير الري بالماء المعالج مغناطيسيا في نمو وحاصل الحنطة Triticum aestivum L Effect of Irrigation With Magnetic Treated Water On Growth And Yield of Wheat (Triticum Aestivum L.)

المستخلص: نفذت التجربة في حقل تابع للمعهد الفني - الكوفة شمال غرب محافظة النجف لموسم النمو (2012 - 2013) بهدف معرفة تاثير نوعية مياه الري وشدة معالجتها مغناطيسيا لثلاثة اصناف من حنطة الخبز Triticum aestivum L. في مؤشرات النمو الخضري والزهري والكيميائي والانتاجي.ص | An experiment was conducted in a field belonging to the Technical Institute - Kufa north west of Najaf governorate during the season of the year (2012 - 2013) to determine the effect of irrigation water quality and intensity of magnetically treated to three cultivars of bread wheat (Triticum aestivum L.) on vegetative growth, flowering, chemical and productive indicators.The experiment was designed randomized complete blocks (RCBD) arrangement Split split - plots and three replicatesfor each treatment. Experiment included study three factors is the quality of irrigation water (river water or well water) - Main plot, wheat bread varieties (IPAA - 99, Rashid and Tamuze - 2) - Sub - plot and magnetic treatment to irrigation water four intensities is (0, 750, 1500 and 3000) Gauss - Sub - sub - plot. The Revised Least Significant Difference (RLSD) at 0.05 probability was used to compare treatment means, whenever treatments affects were evident.Results showed : 1 - Wheat plants irrigated with river water were superior in all parameters in forms of plant height, leaves number, leaf area, tillers number; productive and non - productive, root length, lateral root length of the plant, leaves content from total chlorophyll, dry weights for plant vegetative, phosphorus% in grains, grains weight of spike, spike number of plant and m2, weight of 1000 grain, plant yield of grains, m2 yield of grains, hectare yield of grains per hectare compare to plants irrigated with well water, which recorded the highest chemical content of grains from nitrogen, potassium, magnesium, protein and carbohydrates percentages. The percentage of flowering, spikes length, spikes grains number were superior in the beginning with the well water, were superior at the end with river water.2 - Magnetic treatment with irrigation water by 1500 gauss revealed that noticeable superior was obtained in most of plants vegetative and floral characteristics, while the chemical characteristics had increased with magnetic water treatment by 3000 gauss and yield characteristics treated with 750 gauss. The effect of magnetic water treatment on leaves content from total chlorophyll and magnesium percentage in grains was negative.3 - The majority of vegetative, chemical and productivity parameters was increased with Rashid plants cultivar compared to same parameters for Tamuze - 2 plants cultivar which superior than IPAA - 99 plants cultivar except number of leaves, tillers number and spike grains number which superior with IPAA - 99 plants cultivar.4 - The triple interaction between experiment all factors revealed a significant effect on magnetized river water at 1500 gauss in Rashid and Tamuz - 2 plants cultivars giving highest vegetative growth indicators average which increased on plant yield with low magnetic water treatment (750 gauss) to Rashid plants cultivars and medium strongly (1500 gauss) for Tamuz - 2 plants cultivars, while the characteristics of grains chemical content were superior with same plants cultivars but magnetized treatment well water at 3000 gauss.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: النبات - الفسلجة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ثامر خضير مرزة
اسم المؤلف: فاضل كاظم كريم الابراهيمي

دراسة تشريحية لبشرة اوراق بعض اجناس العائلة المركبة Compositae (Asteraceae) في جامعة القادسية Anatomical Study of The Leaves Some Genus Compositae (Asteraceae) In Al - Qadisiyah University

المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لصفات البشرة للاوراق بعض الاجناس التابعة للعائلة المركبة (Compositae) وهذه الاجناس هي Aster L. و.Calendula L وDendranthema L. و.Helianthus L و.Lactuca L و.Launaea L و.Tagetes L, وتبين ان لبعض الصفات التشريحية اهمية | The present research includes comparative anatomical study epidermis of some genera which belong to sun flower family (Compositae) These genera are Aster L., Calendula L., Dendranthema L., Helianthus L., Lactuca L., Launaea L. and Tagetes L. , and show that details anatomical the importance of taxonomic possible from isolation genuses more each adjective undulate the walls vertical epidermis the leaves, and we show some importand for indumentum suchas T - shape eglandular haire in Dendranthema L. species
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دبلوم عالي
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ازهار عبد الامير سوسة
اسم المؤلف: فرقد حيدر عبد النبي المحنة

التحري عن جينات المقاومة لبعض المضادات في Candida spp. المسببة لداء المبيضات Detection On Resistant Genes To Some Antifungal In Candida Spp. That Causing Candidiasis

المستخلص: تم جمع 107 عينة من حالات سريرية مختلفة شملت 36 مسحة فموية من اطفال مصابين بالسلاق الفموي, 32 مسحة مهبلية من النساء المصابات بداء المبيضات المهبلي و39 عينة بولية من اشخاص مصابين بالتهاب المجاري البولية (راجعوا مستشفى الديوانية التعليمي العام ومستشفى النسائ | The present study was aimed to determine ERG11 - 2 that responsible for resistance to some antifungal agents in candida spp. Atotal of 107 samples were obtained fromdifferent clinical cases included(36 oral swabs from children suffering from oral thrushwho attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya, 32 vaginal swabs from women suffering from vaginal candidiasis who attending clinics in AL - Diwaniya, 39 urine samples from individuals suffering from urinary tract infection who attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya) and 25 samples from sewage water as a control samples in the molecular study.The result of morphological and biochemical tests showed For pathogenic samples that 83(77.5%) isolate of candida spp. Included 36/31(86.1%) from oral samples, 32/28 (75%) from vaginal swabs, 28/39 isolates (71.7%) from urine samples while the result of non - pathogenic samples revealed that 20 (80%) isolate of candida spp. Detecting of, ERG11 - 2 resistant gene was done by RealTime - PCR technique. Results showed that all isolates 31 (100%) were carrying ERG11 - 2 that responsibe of resistance in candida to some antifungal, Did not record any isolated from control samples from sewage water which amounted to (9) the ERG11 - 2 resistant
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: علي حمود السعدي نيران عبيد جاسم
اسم المؤلف: رجاء علي حبيب ظاهر الخزاعي

دراسة جرثومتي السالمونيلا والشيكلا المعزولة من حالات سريرية في مدينة الديوانية وتاثيرهما على معايير دم المرضى

المستخلص: Salmonella is a bacteria and Shigella types of intestinal bacteria, and because of the seriousness of the pathogenicity and the spread of infection in their environment has been conducting this study.Collected during the current study, 462 different clinical sample (feces and urine) of adults aged 20 years and older and those with symptoms of the bacteria Salmonella and Shigella and both sexes reviewers for laboratories and hospitals in the city of Diwaniya, for the period from 2012 \ 11 \ 6 to 2013 \ 5 \ 26. Diagnosed isolates based on phenotypic traits and AGRO tests biochemical in addition to diagnosis by Api20E system as isolates Salmonella reached 17 isolation and by 3.6% and were all belonging to the type S.typhi, either isolates bacteria Shigella was three isolates and 0.64%, and all of them belong to the type of Sh. Flexneri. The incidence of bacteria S.typhi for the male sex was higher than in females, Ozbulg number of infected males and 10 by 58.8%, while the number Fayalnads was infected 7 and by 41.1%, but for bacteria to Sh. flexneri, as was the incidence in males is also higher than in females as in males reached 2 and by 66.6%, while the number of infected females in bringing only one injury and 33.3%. Were more age groups injury is a class 20 - 29 where recorded 9 injuries and by 52.9% and the number of infected males 6 The number of cases in females 3 injuries, age group 30 - 39 recorded 5 injuries and by 29.4 and were two goals in the male and three injuries in females, the age category 40 - 49sgelt two goals by 11.9% and the injury was confined to males either category above 50 years and one injury recorded only in females and 5.8%, either in bacteria Sh. flexneri was the only injury in the 20 - 29 age group and by three injuries. The more isolated the proportion of bacteria S.typhi in the month of May and April as recorded 4 isolates each, and by 23.5%, while giving the lowest ratio for the presence of bacterial during the months of December and January, as recorded isolation and one each of these two months and 5.8% either November remaining months February, March was the number of isolates in 3, 2, 2, respectively, and 15%, 17.6%, 11.7, 11.7%, respectively, as well, either for the bacteria Sh. flexneri as Bulgtaaly proportion of injury in the month of May was the number of isolates 2 and by 66.6% during the month of April either recorded a hit single Vqtobnsph 33.3% did not notice any injury to the other months of the study. Tested the sensitivity of the isolates S.typhi and Sh.flexneri direction antibiotics used as 8 used antibiotics in common use in this study show that the bacteria were sensitive S.typhi 100% Anti Ciprofloxacin, but resisted antibiotics Ampicillin, Amoxicillin, Chloramphenicol, Tetracycline, Nalidixicacid, Gentamicin, Ceftriaxone at a rate of 82.3%, 82.3%, 76.4%, 70.5%, 64.7%, 23.5%, 23.5% respectively. The bacteria Sh.flexneri was 100% sensitive to antibiotics Ciprofloxacin, Gentamicin, Nalidixic acid, Amoxicillin, Ceftriaxone while it was resistant to antibiotics Tetracycline, Chloramphenicol, Ampicillin by 66.6% each. Been investigating some of the virulence of the bacteria S.typhi factors, including the presence of the portfolio Capsule as were all Salmonella isolates characterized by possessing the portfolio, while the production of the enzyme protease Protase was all isolates S.typhi producing this enzyme also showed 12 isolation of the ability to produce the enzyme Lipase and increased by 70.5%, but all isolates were S.typhi unproductive enzyme protease. The bacteria Sh.flexneri marked by Azltan of three Azladtha and by (% 66.6) viability link red dye Congo also showed 2 of these isolates and by (66.6) ability to form thin membrane (Pellicle) and two Aezzltan which have demonstrated susceptibility to adhesion was isolated one of them only possess cilia first pattern type 1. The research covered also study the changes that occur in some of the blood parameters in people infected with the bacterium Salmonella Heclauzar that there is a decrease in the total number of red blood cells Red blood cells in people infected with Salmonella, where the total number of blood cells Reds reached 4.91 x 106 c \ ml total number of cells rate 3.7 white blood x 103c \ ml the proportion of types of white blood cells of the total number of cells, the white was lymphocyte ratio lymphocytes% 52.55 which is low compared with the normal number, while there was an increase in both acidophilus cells Eosinophils and only Monocyte as she was represented 1.2%, 2.9% in November while it was% 0.03 Basophils cells, the highest percentage of increase in healthy people, and the amount of total hemoglobin rate. Hemoglobin9.07 g \ dl which is low compared with healthy peopleBut in people infected with bacteria Sh. Flexner, bringing the average number of red blood cells 106 c \ ml3.7 x, the lowest rate with the number of healthy people, as the amount of hemoglobin g \ dl13.5 rate, either the total number of white blood cells, the rate was slightly higher than what is found in healthy people Azbulg (11.32 x 103c \ ml), it was noted that most of the increase was high in the white blood cells of the type Neutrophils the increase amounted to 62.34%
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: علي عبد رحيم الناشي
اسم المؤلف: هبة اركان فنطيل الحسناوي

تاثير الكثافة النباتية ومستويات التسميد في نمو وحاصل اصناف مختلفة من نبات زهرة الشمس (Helianthus annuus L.) Effect of Plant Density And Fertilization Levels In Growth And Yield of Sunflower (Helianthus Annuus L.) Cultivars

المستخلص: اجريت تجربة حقلية في محطة ابحاث كلية الزراعة / جامعة ديالى اثناء العروة الربيعية للعام 2014 في تربة مزيجية غرينية, تمت الزراعة بتاريخ 2014/2/23. نفذت التجربة باستخدام تصميم القطاعات العشوائية الكاملة R.C.B.D وبنظام تجربة عاملية بتنظيم القطع المنشقة المنشق | This experimet was carried out in the experiments station of the Agriculture College/ University of Diyala in the spring season of 2014 in 23/2/2014, using silty loam soil. Experiment was on sunflower with a factorial experiment, a split split plot randomized compelete block design with three replications. This study included three factors, first the NPK fertilizer with three levels (0, 150, 300) Kg/h - 1 which were divided into two parts first 30 days after seeds germinated and the second 35 days after the first. The second factor was the plants density with three levels (66666, 80000, 100000) plants/h - 1 and the third factor three varieties of sunflower (Shemoos, Akmar, Euroflour) , and the important results obtained were : 1. The highest density level (100000) plants/h - 1 gave the highest results in most of the characters studied which were, head or disk diameter, plant yield, yield (ton / h - 1), fertility percentage, oil percentage in seeds, oil yield(ton / h - 1), and protien yield (ton / h - 1).2. Level of 150 Kg/ h - 1 of NPK fertilizer was the best in most of the characters which were head diameter, plant yield, yield (ton / h - 1), oil percentage, oil yield (ton / h - 1), protien percentage and protien yield (ton / h - 1).3. Shemoos variety exceeded other varaieties in leaf area, leaf area index, stem diameter, head diameter, 1000 seed weight, plant yield, yield (ton / h - 1), seeds number in head, oil yield (ton / h - 1), protien yield (ton / h - 1) , but it gave the highest number of days to flower and mature.4. The interaction (Shemoos variety X zero NPK) gave significant highest mean in number of days from planting date to 75% flowering.The interaction (Akmar variety X 150 Kg NPK / h - 1) gave the highest values in stem diameter, head diameter, lodging percentage, but the interaction (Shemoos variety X 300 Kg NPK / h - 1) gave the highest values in leaf area, leaf area index, and number of days from planting date to maturity.5. The interaction (Shemoos variety X 66666 plants per hectar) gave significant differences in number of seeds per head , 1000 seeds weight, one plant yield, and yield (ton / h - 1). The interaction (Akmar variety X 100000 plants/ h - 1) gave significant differences in fertility percentage.6. The interaction (150 Kg NPK / h - 1 X 66666 plants/ h - 1 ) gave the highest significant differences in all quality characters studied. 7. The interaction (Shemoos variety X150 Kg NPK / h - 1 X 100000 plants/ h - 1) gave the highest significant results in seeds yield, oil yield and protien yield.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: نجم عبد الله جمعة الزبيدي
اسم المؤلف: محمد سلمان كريم الزبيدي

دراسة التاثير الاليلوباثي لنبات اليوكالبتوس Eucalyptus sargentii في انبات ونمو بعض نباتات الزينة The Allelopathic Effect of Eucalyptus Sargentii On Germination And Growth of Some Ornamental Plants

المستخلص: اجريت تجربتين, حقلية ومختبرية, لدراسة التاثير الاليلوباثي لمسحوق الاوراق الجافة او الطرية لنبات اليوكالبتوس ومستخلصاتها (المستخلص الايثانولي, الزيت, التربينات, القلويدات والفينولات) في الانبات وصفات النمو الخضري والزهري ومحتوى بعض العناصر المعدنية, السكري | Two experiments were carried out, field and laboratory experiment, to study the allelopathic effect of dry or fresh leaves and their extracts of Eucalyptus sp. on four ornamental plants; Calendula officinalis, Tagetes patula, Mathiola incana and Asteriscus graveolens Less. The two experiments were conducted at the field of College of Agriculture/Al - Qadisiya University during the period from 31/10/2012 to 14/3/2013. For the field experiment, dry and fresh leaves of Eucalyptus were used at the rate of 0, 2, 3, or 6 g dry leaves/Kg soil or at the rate of 0, 4, 8, or 12g fresh leaves/kg soil. Plant height, changes in height during plant life time - course, number of branches, number of flowers, and flower diameter were measured. In addition, chlorophyll content in leaves, anthocyanin in petals, dry weight, nitrogen percent, potassium and phosphorous content in leaves were also determined. For the laboratory experiment, ethanol extract, oil, terpenes, alkaloid, and phenols extracted from Eucalyptus leaves at different concentrations were used. Seed germination, coefficient velocity of germination, plumule and radical length, sugar, protein, melanodialdehyde, glutathione and proline content were evaluated. The results were as follow : 1. Ethanolic extract was negatively affect the percent and coefficient velocity of germination in addition to plumule and radicle length. The reduction in these parameters was greater at the highest concentration of the extract, especially in Calendula officinalis. 2. Treatment with oil extracted from Eucalyptus leaves caused a significant reduction in percent and coefficient velocity of germination, plumule and radical length. 3. Terpenes, alkaloids or phenols treatments at different concentrations resulted in significant reduction in percent and coefficient velocity of germination, plumule and radical length. The reduction was increased as the concentration of the treatments increased. 4. Plant height decreased significantly due to the treatment with dry or fresh Eucalyptus leaves, the reduction in plant height increased as the concentration of the treatment increases in all plants except Asteriscus graveolens plant which showed that the small concentrations of both treatments. It was close to ward in an increase in plant height. 5. Number of branches also significantly decreased when ornamental plants treated with dry or fresh Eucalyptus leaves; the Calendula officinalis plant was the most affected. 6. For the changes in ornamental plants height, as a result of treatment with dry or fresh leaves of Eucalyptus, during life time - course, it was clear the growth curve was close to the S - shape growth curve with obvious significant differences for most treatments compare to control. 7. Dry weight percent was significantly affected in all ornamental plants when they treated with dry or fresh Eucalyptus leaves, If decreased with the concentration of the treatments increased. 8. It was obvious that treatment with dry or fresh Eucalyptus leaves resulted in significant reduction in flower number and flower diameter. Number of flowers decreased as the concentration of the treatments increased. on the other hand, flower was diameter increased as the concentration of the treatments increased, but in both cases it was less than the control. All one obvious that reducing significant in flower diameter, It was reducing great in low levels. 9. Chlorophyll content was also decreased in leaves, while anthocyanin content in petals was increased when the ornamental plants treated with dry or fresh Eucalyptus leaves. 10. Nirogen percent, phosphorus and potassium content in leaves of the four ornamentals decreased when they treated with dry or fresh Eucalyptus leaves. 11. Using terpenes, alkaloids and phenols caused significant increase in sugar content in leaves of the ornamentals, reaching its highest values at the highest concentration of the terpenes. For protein content, it was gradually decreased as the terpens, alkaloids and phenols concentrations increased in Calendula officinalis. In Tagetes patula, protein content was noticeably increased with the increasing in concentrations of the treatments. 12. Malondialdehyde, glutathione and proline content increased significantly in Calendula officinalis and Tagetes patula plants when they treated with terpenes, alkaloids or phenols. 13. Interaction between treatments concentrations and kind of plants had significant effect on all parameters measured.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: مجيد كاظم عباس الحمزاوي
اسم المؤلف: كوثر هاشم عبار الجاسمي

دراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر الثقيلة في مياه نهر الديوانية - العراق A Study of Structural Phytoplankton And Their Relationship With The Environmental Conditions And Some Heavy Metals In Al - Diwaniya River /Iraq

المستخلص: تناولت الدراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر النزرة في مياه نهر الديوانية, اذ جمعت عينات الدراسة (المياه والرواسب والهائمات النباتية) شهريا من ثلاث محطات على نهر الديوانية ابتداء من تشرين الاول 2012 ولغاية ايلول 2013، وشمل | This investigation was designated to study of structural of phytoplankton and its relationship with environmental conditions and some of heavy metal in AL - Diwaniya river, the samples were collected monthly for water, sediments and phytoplankton from October 2012 till September 2013, the study included the measurement of the temperature of air and water; Light penetration; salinity ;electrical conductivity ; T.S.S;T.D.S ;Turbidity; pH ;Oxygen Dissolved; Biological Oxygen Demand ;Chemical Oxygen demand ;Dioxide carbon ;AL - alkalinity; Hardness; Ca; Mg; and nutrients(nitrate; nitrite; phosphate) ; total organic carbon) and the study also included the measurement of some heavy metals (Cadmium, lead, copper, Zinc) in dissolved and particular parts of water and in sediment both exchangeable and residual parts and also studied the structure of phytoplankton quantity and quality.Results of the study showed that the temperature of air and water ranged between (15 - 43) ?C (13 - 33)?C respectively. It was found the light penetration ranged from (27.2 - 100), conductivity was (1264 - 1555)?S/cm, while the salinity was (0.80 - 0.99)%, pH was ranged between (7.37 - 7.82), while Turbidity was ranged between(43.6 - 59.02) NTU, total dissolve substances was (985 - 1411) mg/L while total suspend solid substances was (40 - 66) mg/l. The dissolved Oxygen was within the range of (6.3 - 8.26) mg/l, the biochemical and chemical Oxygen demand was (1.19 - 3.08) mg/l (12.4 - 38.01) mg/l respectively while dioxide carbon was ranged(80.5 - 134.2)mg/l.The study revealed that the river water was alkalinity and very hard, as the range of total alkalinity, total hardness, Calcium and magnesium was (131.5 - 227.4, 312.3 - 458.5, 86.4 - 142, 54.8 - 76.8) mg/ respectively, while the concentrations of the nutrients were showed fluctuated in concentrations, it was (102.6 - 558.4, 1.5 - 3.4, ND - 2.9) ?g/ for Nitrate, Nitrite and Phosphate respectively, total organic carbon was between (0.13 - 0.55) %Seven units were diagnosed during the period of study Bacillriophyceae; Chlorophyceae; Cyanophyceae; Euglenophycae; Dinophycae; Chrysophycae ;Cryptophyceae.Species number of phytoplankton that identified through study period were (134, 101, 96) in three sites respectively, and showed that dominance of Bacillriophyceae (diatoms) which was (71, 50, 46) species fallowed by Chlorophyceae (35, 25, 25) species then Cyanophycae (22, 18, 20) species, Euglenophycae (2, 5, 2) species and Dinophycae(2, 3, 1) species Chrysophycae (1, 0, 2) and finally Cryptophyceae (1, 0, 1)species in three sites respectively,. Generally the most common genera in study period are (Nitzschia, Surirella, Nivicula, Navicula, Syndra, Pediastrum, Scenedesmus, Aphanocapsa, Microcystis, Cocconeis, ,).The total number of phytoplankton was low and ranged between (422.21 - 1027.82) cell ?103/l. This study determined means of heavy metals (Cd, Zn, pb, Cu) in dissolved part was (ND - 5, 4.07 - 29.8 ND - 6.89, 2.08 - 8. 71) ?g/l respectively, while particulate part was) 2.09 - 30.16, 44.87 - 372.17, 3.13 - 90.93, 27.41 - 88.20) ?g/g respectively The study revealed also that the concentrations of heavy metals in sedimentswas (ND - 12.71, 11.76 - 39.93, 0.55 - 6.12, 9.31 - 39.73), ?g/g of exchangeable parts and was (1.09 - 17.94, 39.21 - 172.30, 21.21 - 67.96, 33.96 - 76.63) ?g/g of residual parts respectively. The Statistical results showed there are morale differences between locations and seasons in relation to physical and chemical features, organic compounds, heavy metals and phytoplankton. There are negative and positive, morale correlations between phytoplankton and physical, chemical features and heavy metals that measured during study period at probability at the level of (p<0.05).
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: فؤاد منحر علكم
اسم المؤلف: نور ناظم جابر الميالي

دراسة تصنيفية لبعض مراتب الجنس Ranunculus L. (Ranunculaceae في العراق A Taxonomic Study of Some Taxa of The Genus Ranunculus L. (Ranunculaceae) In Iraq

المستخلص: “The current research dealed with fourteen taxa belonging to the genus Ranunculus L., Family Ranunculaceae that grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also were studied and the chemical compounds (Flavonoids), Ecological and geographical distribution and taxonomic treatment have been done. Also botanical key was puted to separate the Taxa”.“General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens in the Iraqi herbaria were studied after ensuring that it was correctly identified”. “Comparative morphological study was undertaken to all plants parts of taxa including the roots, leaves, stipules, flowering parts and fruiting parts. Charts, diagrams, tables were drawn and accompanying with natural images for different morphological characteristics, as well as the field images that are important for the study. This study showed that all the taxa of the genus were herbs that are annual or perennial. There was agreat important for the roots, stems and there Indumentum, the presence orabsence of stipules, shape and size and margin of basal leaves and cauline leaves and leafy bract, the shape of sepals and their numbers and their Indumentum, the numbers and shape of Petals and their colors, the number of Stamens and the colors of Anthers, the shape of ovary and their indumentum, presence or absence of style as it was very useful for the taxonomic identification and separation of the studied taxa. As well as the fruiting parts including the length of fruiting peduncle, the fruiting receptacle, the fruiting head and its shapes and their dimensions, the number of Achene Fruitlets and their sizes, colors and Beak shape. Also the surface configuration of the fruitlets Surface has been studied by (SEM) as it wae either hairy or tuberculate - hairy or Transversely rugose or muricate or reticulate or reticulate - minutly rugose or granular or granular - hairy or granular - hairy tuberculate or spinulose”.“The study of the micro - morphological characters of hairs, leaves epidermal cells and the stomatal complex, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa”.“The study show that the pollen grains have a taxonomic importance because of their difference in shape, dimensions, the wall thickness and their ornamentation. It was either apolar which was spheroidal and pantoporate, or isopolar which has different shapes in the equatorial view it was spheroidal, subprolate and oblate - spheroidal, it was either 3 - colpate, 4 - colpate or 5 - colpat.the colpate where either zonocolpate or syncolpate in the form of parasyncolpate. The ornamentation were verucate, echinateand scabrate”. “The chemical study also has an importance in separation of the genus taxa, six flavonoid compounds were diagnosed by using” HPLC wich are Kaempferol, Vitexin, Orientin, Quercetin, Iso - vitexin and Iso - orientin. According to the presence or absence of those structures cluster analysis where done for the results of the flavonoid compounds and the taxa where divided into groups that reveal the chemical relationships between them.dendogram where performed which also show the chemical relationships between the taxa by using UPGMA method and NTSYS program issue 21. the study shows that the presence and distribution of those compounds vary according to the variation in the plant distribution the study shows that some of the taxa have “the same compounds which enhance its belonging to the Ranunculus genus”. “Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical. Most of the taxa were distributed in N. and N.E of Iraq regions districts especially in MAM, MSU, MRO, and FBF districts. We notice that MSU district has a great number of the plants under the study as we found there are all the taxa accept one”. This study helps to detect distribution areas for some taxa that have been not mentioned in the Iraqi flora or other records which are R.kotschyi and R.trichophyllus var.trichophyllus. Also the study records distribution for the new species R.chius in MAM district which hasn't been mentioned in the Iraqi flora in this district before. Finally all the taxa were classified and the botanical key was done to separate them.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: مازن نواف عبود العاني
اسم المؤلف: علي طالب محمد الطائي

البدانة عامل خطورة للاصابة بامراض القلب التاجية Obesity As A Risk Factor For Coronary Heart Disease

المستخلص: هناك مفهوم متنام يشير الى زيادة انتشار البدانة بين مرضى القلب التاجية Coronary heart disease (CHD), مما له الاثر الكبير في نشوء المرض ومضاعفاته, اذ يشابه عمل الخلية الدهنية adipocyteعضو الافراز الداخلي endocrine organ.ولمعرفة العلاقة بين البدانة وامراض | There is a growing concept that refers to the increased prevalence of obesity among patients with coronary heart disease. This has significant impact on the emergence of the disease and its complications as the work of the adipocyte resembles the work of the endocrine organ. This study comes to know the relationship between obesity and heart disease. To accomplish this, about 5cm3 of venous blood has been collected from 240 patients of coronary heart disease who are admitted to the resuscitation unit in Baquba Teaching hospital for the period 1st of December 2014 to1st of January 2015. The range of the patients' age is between 22 - 90 years old. The sample has been divided into three groups in accordance with body mass index and according to the classification of the American Institute. These groups are : natural weight from 18.5 - 24.9kg/m2, overweight from 25.00 - 29.9 kg/m2 and obesity30.00 kg/m2. The result shows that there is a significant rise in the fasting blood sugar, the total protein in serum and the systolic pressure with the rise of the body mass. While the level of the cholesterol, triacylgcerol and the remnant lipoprotein of the patient has not been affected with the different of the body mass. The patients with natural body mass index show significant rise in the total and direct bilirubin levels as compared with other groups with no significant differences among the groups in the levels of uric acid, urea, creatine, and phosphorus and iron ionic. We conclude that there is a correlation between the body mass index and the systolic and diastolic blood pressure.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - الفسلجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: حميد محمود مجيد
اسم المؤلف: نور غسان كاظم الدليمي

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بداء السكري النوع الثاني في محافظة ديالى A Study Some Immunological Parameters In Some Patients of Diabetes Mellitus Type Two In Diyala Province

المستخلص: Type II diabetes mellitus is achronic diseas characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both and tissue resistante to insuline. This study was conducted in the Diyala province for the Type II diabetes patients which lasted for the period from beginning October 4102 to the end of May 4102 which aimed to evaluate some immunological indicators for patients infected with diabetes type two which included { assessment of the humoral immune by measuring the level of immunoglobulin (IgM, IgG) and the level of complement protein (C3, C2) by using the single radial immune diffusion assay and measuring the C - reactive protein (CRP) and determine the levels of Interlukin (IL - 01, IL - 01) by ELISA}. Atotal of (011) ablood sample was collected from patients with diabetes type two after diagnosis by a physician specialist in the clinic consulting in Baquba teaching hospital according to the criteria adopted by the World Health Organization for the diagnosis of diabetes type two, has been the number of males (24) percentage (20.11)% and the number of femals (22) percentage (21.02)% within the range of my age (32 - 11) years and collected (11) of blood sample from healthy volunteers ostensibly have been accredited as a group control, has been the number of males (32) percentage (21.33)% and the number of females is (42) percentage (20.14)% within the range of my age (31 - 44) years.Results of the study showed that the incidence of the disease rate a mong females (21.02)% than males (20.11)% with no significant difference statistically between the two groups of study for the sex, has found that age group (24 - 11, 11<) years recorded a high rate of disease (33.14)% and(32.12)% respectively while the age group (31 - 21) years percentage (1.11)% recorded lowest rate of disease whith high significant difference was noticed between the two groups.Furthermore results of the study showed low levels of immunoglobulin (IgM, IgG) in patients compared to the control group where found that the level IgM in patients (043.20124.22) mg/dl compared to the control group (434.24121.24) mg/dl and that the level of IgG in patients (0124.2110123.21) mg/dl compared to the control group (0103.32 ± 0442.14) mg/dl the results also showed low levels of complement proteins C3, C2 in patients compared to the control group it was found that the level of C3 in patients (01.00 ±31.14) mg/dl compared to the control group (030.02 ± 01.24) mg/dl and the level of C2 in patients (02.31 ± 4.11) mg/dl compared to the control group (20.231 4.13) mg/dl, and the results showed that the positivity of C - reactive protein in patients was (41.24)% compared to the control group was (1)% The results of the current study showed high level of IL - 01 in patients (410.24 ± 011.40) pg/ml compared to the control group (14.11132.43) pg/ml and the level of IL - 01 the results showed alow level of the IL - 01 in patients (13.13 ± 20.01) pg/ml compared to the control group (43.04 ± 40.03) pg/ml, inaddition to the study result showed agreat significant correlation between complement proteins (C3, C2) and the humoral immune (immunoglobulin IgM, IgG) as well as acorrelation between cellular immunity (IL - 01)and (IgM, C3).
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: محمد عبد الدايم صالح
اسم المؤلف: ريم قاسم محمد الحديدي

تقييم فعالية المستخلص المائي والكحولي لنبات الصبار Aloevera في الاصابة التجريبية لطفيلي Eimeria tenella لدى فروج اللحم Evaluationof Alcoholic Andwateryextracts Efficacyofaloe Veraon Experimental Infectedbroilerwitheimeriatenella

المستخلص: اجريت الدراسة الحالية على (216) فرخ دجاج نوع Rose 308مصاب بطفيلي Emiria tenella خلال المدة من شهر نيسان 2013 الى شهر تشرين الاول 2013, وقد تم في الدراسة الحالية تحديد بعـــــــــض المؤشــــــــــــــرات الدمــــوية (الخلايا المتغايرة /الخلايا اللمفاوية HL | This study was conducted to (216) broilers chickens (Rose 308 Trade) infected parasite with Eimeria tenella performed form April 2013 till October 2013, has been in the current study to identify some indicators of blood(Heterophils/ Lymphocytes, White Blood Cell, Red Blood Cell, Packed Cell Volume, Hemoglobin) and biochemistry (Glucose, cholesterol and total protein) and productive performance (Weight increase, Food conservation, Cansumbtion and Body weight befor slugtery) , We note at the parasite has infected chicks were used three extracts of Aloe vera (alcoholic and hot water and cold water), dosage chicks were to demonstrate the effect of different concentrations of these extracts on the productivity and physiological traits mentioned above. Then the experiment was designed, which include the following : 1 - The first group (control group) included 36 chicks and was given food with no anti - coccidial. 2 - The second group include 36 chicks in duplicate was infected but did not treated.3 - The third group inclouded 36 chicks in duplicate was given extract of Aloe vera Alcoholic ethyl in adose of (0.5 - 1.5 - 2)gm per mm body weight.4 - The fourth group inclouded 36 chicks in duplicate was given extract of Aloe vera Cold water in adose of (0.5 - 1.5 - 2)gm per mm body weight.5 - The fifth group inclouded 36 chicks in duplicate was given extract of Aloe vera Hot water in adose of (0.5 - 1.5 - 2)gm per mm body weight.6 - The sixth group include 36 chicks in duplicate was given Vetacox(trade Mark)that had both Sulphadimidin 80 gm and Diverdin8 gm.Results showed the following : - 1 - The alcoholic extract of Aloe vera at concentration (0.5, 1.5, 2) g/ml after an infection in the fifth week revealed alittle effect on the H/L when compared with eighth week of infection. also this extract lead to increase, the rate of (PCV), (Hb) , and (RBC) while lead to decrease the rate of (WBC) after eighth week of infection at concentration (2)g/ml. Also this extract lead to increase the rate of cholesterol, total protein and the rate of weight increased in the eighth week, while the amount of feed intake and feed conversion showed alittle effect, as well as the body weight befors slaughter has astrong effect at concentration(2)g/ml in the eighth week of infection. 2 - The cold and hot aquatic extract of Aloe vera at concentration(2)g/ml lead to decrease the ratio of H/L and increase the rate of (PCV), (Hb) and (RBC) after the infection in the eighth week, also lead to decreas the rate of (WBC) and increase the rate of cholesterol, total protein, the rate of weight increased, the amount of feed intake and the body weight before slaughter at concentration (2)g/ml in the eighth week of infection. Also recorded abetter efficiency of feed conrersion at concentration (2)g/ml in the eighth week of infection.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: هيثم محمد حمادي العوادي
اسم المؤلف: زهراء حاتم حميدي عبود الكعبي

دراسة تصنيفية حياتية مقارنة لمراتب من الجنس Onobrychis Mill. (Fabaceae) في العراق

المستخلص: استــهدف البــحث الــحالي دراســة تصــنــيفية مقارنة لاحدى عشر مرتبة تصنيفية تابعة للجنس Onobrychis Mill. من العائلة الفراشية (البقولية) Papilionaceae النامية بريا في العراق وهي O. acaulis وO. caput - galli وO. carduchorum وO. crista - galli بضربيه O. cri | A comparative systematic study was carried out for eleven taxa belong to the genus Onobrychis Mill., Family Papilionaceae grown widely in Iraq, and they are O. acaulis, O. caput - galli, O. carduchorum, O. crista - galli with two varieties O. crista - galli var. crista - galli and O. crista - galli var. trilophocarpa, O. galegifolia, O. haussknechtii, O. kotschyana, O. megataphros, O. ptolemaica, O. schahuensis. The Study included the gross general Morphological characters and Micromorphological characters, also the Pollen grains studied and Anatomy, Chemistry, Enviroment and Geographical distribution and Taxonomic treatment, also botanical key was putted to separate the taxa. General survey for the Phytogeographical districts where the taxa of genus distributed, in order to detect the distribution of the studied taxa of the genus Onobrychis. Therefore many collections obtained and new locations of taxa distribution were recorded, the specimens studied, and correct the mistake naming specimens were identified and reidentified some miss identified specimens. A comparative morphological study was undertaken to all taxa plants parts include the root, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying, with natural images for different morphology characteristics, as well as the field images. This study indicates that the characters of Leaf, Inflorescence, Flowers and Fruit were collectively contributed in the separation and identification of taxa. The pollen grains have a taxonomic importance because their different shape, dimensions of polar and equilateral view and wall thickness. The anatomical study showed the importance of Taxonomic support to the morphological characters in the diagnosis and isolation different taxa, and this study showed different in the anatomical characters of leaf include the characters of Epidermis, Stomata and the characters of a cross sections in stems. The current study showed that the trichomes were very important taxonomic in isolate different taxa because their variation in density, dimensions and the shape of epidermis cells that the trichomes arise from them and dimensions of the epidermal cells and the nature of their walls. The current study identified nineteen protein - amino acids by using High - Performance Liquid Chromatography (HPLC) and the distribution of these compound was variable among various taxa of the genus and that was useful in isolation different taxa. and the aliphatic amino acids, Glutamic acid, Glycine, Serine, Arginine, Alanine, Tyrosine, Valine, Methionine, Isoleucine and Lysine had a wide presence in the studied taxa, which means that they belong to the same genus. The ecology and geographic distribution of the taxa were studied using area survey and information of herbaria specimens, maps have been drawn for taxa distribution in the Iraqi natural geographical provinces. At last, a taxonomic treatment has been carried out for the taxa of the genus Onobrychis, as well as a detailed key to isolate them.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عذية ناهي سلمان المشهداني
اسم المؤلف: زينب جواد عامر

تاثير المياه الممغنطة والبنزل ادنين ومستحضر السماد الطحلبي في نمو نبات الجزر Daucus carota L. Effect of Irrigation Water Magnetic, Benzyladenine And Algal Fertilizer Preparation On Growth of Carrot Plant Daucus Carota L

المستخلص: نفذت التجربة باستعمال الاصص خلال الموسم الشتوي للعام (2012 - 2013) م في تربة مزيجية وتحت الظروف البيئية الطبيعية في قسم علوم الحياة/ كلية التربية/ جامعة القادسية, لمعرفة تاثير مياه السقي الممغنطة والرش بتراكيز من البنزل ادنين ومستحضر السماد الطحلبي في ال | An experiment was conducted in the winter season of the year (2012 - 2013) in loam soil pots, in Biology department/ College of education/ Al - Qdisiya university, to find out effect of irrigation water magnetic, spryer of benzyl adenine concentrations and algal fertilizer on growth, mineral content and carotenoids to carrot plant (Daucus carota L.).The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement with three factors. The first factor was four intensities of magnetic to irrigation water (0, 500, 750 and 1000) gauss, the second factor was three concentration of benzyl adenine (0, 50 and 100) mg.L - 1 and third factor was three concentration of algal fertilizer (0, 1.5, and 3) ml.L - 1, with three replicates. The treatments means were compared by using averages revised least significant difference (RLSD) at 0.05 probability level.The following parameters were measured during the study plant height, leaf area, leaves number, vegetative fresh and dry weight, root dry weight, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, in addition to percentage of NPK, total protein and Fe (µg. g - 1). Results showed : 1 - The use of magnetic water by 1000 gauss were increase in plant height, leaf area, leaves number, vegetative fresh and dry weight, root dry weight, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, and magnetic water by 750 gauss were increase vegetative fresh weight and 500 gauss was increase K% but N%, P%, Fe (µg. g - 1) and protein% not affected by any different intensities to irrigated water.2 - Benzyl adenine at 100 mg. L - 1 concentration increased plant height, leaf area, leaves number, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, while 50 mg. L - 1 of benzyl adenine caused by increased in vegetative fresh and dry weight, N%, P%, Fe (µg. g - 1) and protein% but K% not affected.3 - The use of 3 ml.L - 1 algal fertilizer increased plant height, vegetative dry weight, P%, Fe (µg. g - 1), Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul. The concentration 1.5 ml.L - 1 algal fertilizer was increased leaves number but not affected in vegetative fresh weight, N%, P%, K% and protein%. The interactions with another factors caused increase of percentages. 4 - The interaction between magnetic water by 1000 gauss with 100 mg. L - 1 benzyl adenine increased plant height, leaf area, leaves number, leaves content of chlorophyll (a and total chlorophyll), carotenoids, Baicalin and Pycogenul. The use of magnetic water by same intensity (1000 gauss) with 50 mg. L - 1 BA increased vegetative dry weight, N%, P% and protein% while b chlorophyll was increase with 500 gauss and 100 mg. L - 1 BA.5 - the interaction between magnetic water and algal fertilizer concentrations revealed that use of 1000 gauss with 3 ml. L - 1 algal fertilizer increased plant height and leaf area, but the same interaction with 1.5 ml. L - 1 algal fertilizer increased leaves number, leaves content of chlorophyll (a and total chlorophyll), carotenoids, Baicalin and Pycogenul.6 - The interaction between benzyl adenine and algal fertilizer showed that used 100 mg.L - 1 BA with 3 ml. L - 1 algal fertilizer increased b chlorophyll, carotenoids and Baicalin, but use of the combination consisting of 100 ml. L - 1 BA with 1.5 ml/L algal fertilizer increased leaves number, leaves content of chlorophyll (a and total chlorophyll) and Pycogenul.7 - The triple interaction between experiment all factors revealed that increased in vegetative dry weight with two combinations (500 gauss magnetic water + 50 mg. L - 1 BA + 3 ml. L - 1 algal fertilizer and 1000 gauss magnetic water + 50 mg. L - 1 BA + 1.5 ml/L algal fertilizer). High P% was combination consisting of (500 gauss magnetic water + 100 mg.L - 1 BA + 1.5 ml.L - 1 algal fertilizer). High total chlorophyll, carotenoids, Baicalin and Pycogenul were combination consisting of (1000 gauss magnetic water + 100 mg. L - 1 BA + 1.5 ml. L - 1 algal fertilizer).
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات - الفسلجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عبد الامير علي ياسين
اسم المؤلف: سارة عدنان نخاله

دراسة بكتريولوجية كيمياوية وفيزياوية لمياه الشرب المعدنية المتداولة في الاسواق المحلية لمدينة تكريت Bacteriological Chemical And Physical Study for Drinking Mineral Bottled Water Circulating in Local Market in Tikrit City

المستخلص: تضمنت الدراسة الحالية جمع عينات شهرية من اصناف المياه المعدنية المعباة المتداولة في الاسواق المحلية في مدينة تكريت ابتداء من شهر تشرين الاول عام 2010 م ولغاية شهر حزيران عام 2011 م وتم تقييم جودة عشرة اصناف من المياه المعباة بالاضافة الى ماء الصنبور ومقار | The current study included collected monthly samples of mineral water circulating in the local markets in the city of Tikrit from the month of October in 2010 until the month of June in 2011. It was evaluated the quality of ten varieties of bottled water and comparing the results to the standard specifications for Iraqi drinking water and international standards for the World Health Organization.Evaluation included a study of physical, chemical and microbiological factors : turbidity, total dissolved solid, total suspended solid, electrical conductivity, pH, dissolved oxygen, biological oxygen demand, total alkalinity, acidity, total hardness, calcium, sodium, potassium, salinity, chloride, phosphate, nitrate, sulphate, heavy metals, total number of bacteria and total coliforms.The level of quality parameters of these classes were in compliance with the different standards except for pH in one class Pearl and phosphate in one class Rovian.Comparing bottled water to tap water found that the concentrations of turbidity, total dissolved solid, total suspended solid, electrical conductivity, salinity, dissolved oxygen, total hardness, acidity, nitrate, iron, manganese and total number of bacteria in tap water higher than the concentrations in bottled water but didn’t exceed the upper limit that allowed by the local and international specifications for drinking water.Measurements also revealed that the values of most of the criteria listed on the packaging do not reflect the actual content of these water bottles with the exception of one class Kameran most of the packaging in this class has matched the actual content of the water container.The results also showed the absence of coliform bacteria in any of the varieties of bottled water and tap water as well as the significance of the absence of bacterial contamination in it.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: حميد سلمان خميس المهداوي ياسين حسين عويد
اسم المؤلف: اسراء حمود عبد الدوري

مستوى الهستامين وبعض المعايير المناعية لمرضى الحساسية التنفسية من النوع الاول The Level of Histamine And Some Immunological Parameters Among Airway Hypersensitivity Type I Patients

المستخلص: تم جمع 50 عينة دم من مرضى الحساسية التنفسية من النوع الاول المراجعين لمركز الحساسية والربو في محافظة بابل للفترة الممتدة ما بين تشرين الثاني 2013 ولغاية شباط 2014, بالاضافة الى 10 عينات دم من الاشخاص غير المصابين بالحساسية من النوع الاول والذين كان معدل I | Blood samples have been drawn from 50 airway hypersensitivity type I patients who visit the center of the allergies and asthma in the province of Babylon, for the period between November 2013 and up to February 2014, in addition to 10 samples from persons with normal IgE level as a control. The sample was divided into two parts, the first used to measure the level of eosinophils and basophils and the second used to measure the level of immune parameters, which included histamine, IgE, IL - 5, IL - 10 and IL - 12 by using the technique of Enzyme - Linked Immunosorbant Assay (ELISA). Study community were divided into three groups, the first group named before immunotherapy treatment and the second group after immunotherapy treatment and final is for control. Conducted differences in the level of immune parameters between the three groups, there were significant differences between the group before the treatment and control group as well as between the group before treatment and after treatment. The level of histamine for the three groups (before treatment and after the treatment and control), reached respectively 34.8292 ± 6.47680 ng / ml and 9.3238 ± 3.55296 ng / ml and 6.9844 ± 3.05477 ng / ml, and the level of IgE reached to the before treatment group 40.6279 ± 15.24231 IU / ml and the after treatment group 23.9529 ± 11.88933 IU / ml and control group 11.7796 ± 5.53 IU / ml, while the cell count of eosinophils for the before treatment group 0.7283 ? 0.24556 x 103 cells / mm 3 and after treatment group 0.3643 ? 0.14861 x 103 cells / mm 3 and control 0.2252 ? 0.09139 x 103 cells / mm 3 , as well as for the cells of basophils for the before treatment group 0.1008 ? 0.03391 x 103 cells / mm 3 and after treatment group 0.0777 ? 0.03485 x 103 cells / mm 3 and control 0.0724 ? 0.03568 x 103 cells / mm 3 , As for the cytokines the level of IL - 5 reached for the same groups 51.3214 ± 6.18565 pg / ml and 22.7254 ± 3.99360 pg / ml and 24.5404 ± 8.64920 pg / ml respectively, while IL - 10 has reached 14.9604 ± 6.48170 pg / ml and 43.0476 ± 21.26668 pg / ml and 24.4969 ± 10.21066 pg / ml, respectively, and finally the level of IL - 12 reached for the same groups 17.753 ± 8.39343 pg / ml and 39.1929 ± 17.73975 pg / ml and 26.7731 ± 12.93092 pg / ml, respectively. There were been a difference in the level of immune parameters for a number of variables, , place of residence, gender, and type of allergy, there were no significant differences observed in the level of immune parameters between these variables. comparison was made in the level of immune parameters for the group after the treatment depending on the duration of immunotherapy, and found that there were significant differences in the level of these parameters with the increase of the duration of treatment. Through this study, conducted the correlation among histamine and the rest of the immune parameters and the results were pointing to the positive correlation of histamine with IgE, IL - 5, eosinophils and basophils and negative correlation with IL - 10 and IL - 12.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: فريال جميل عبد
اسم المؤلف: سلوى احمد حمزة الكرادي

التشخيص الجزيئي للفطر المحاري Pleuroutus ostreatus وفاعلية بعض المخلفات الصناعية في انتاجه وقابليته الخزنية Molecular Diagnosis of The Oyster Mushroom Pleuroutus Ostreatus And Effectiveness of Some Industrial Waste In Its Production And Its Ability Storage

المستخلص: تضمنت هذه الدراسة انتاج الفطر المحاري ostreatus Pleuroutus باستعمال اوساط زرعية محضرة من مخلفات صناعة محلية وما لها من اهميه من الناحية الاقتصادية كونه يعد عنصرا غذائيا مهما يحل محل البروتين الحيواني المتمثل باللحوم، وما لهذا الفطر من اهمية طبية وعلاجية ا | This study included the production of mushroom oyster Pleuroutus ostreatus by using agricultural media prepared from local industry and which has importance in the economic district it's consider important nutrition element instead of animal protein representive by meat. this fungus has importance of medical and therapeutic, the results showed existence of chemical substances concentrated in the fruiting bodies such as Galic acid and Statin and other substances. that have had an active role in reducing cholesterol in the blood as well as break the clot blood through the presence of certain types of enzymes that break down blood clotting, which may occur, the results showed when studying the mushrooms from the genetic diagnosis molecularly using the technique of the PCR to determine the type of isolation that gives the production of economically significant. Results showed the presence of gene hereditary called Osteareolysin who works with several aspects of the mushrooms which give the recipe contrast pathogenic fungi plant also this study included re - using some of the remnants of plant starch corn husks and Al - gluten and corn embryos. the remnants of Maysan sugar Factory of material Molasses and Molass Baggaz and the study of their role as carrying materials, central ferment and growth of the oyster mushroom during agricultural it on these remnants. The results of the chemical analysis of the remnants of the sugar indestrey in Maysan Molasses and Baggaz and waste plant starch in Al - hashemeyeh / Babylon Al - Baggaz and yellow corn straw showed that molasses substance was contain the amount of sugars which sometimes reach to 41%, that making it as a good base of food to mushroom. When added molasses at different levels 2, 4, 6% to the central media the study showed a positive relationship between the levels of molasses and the radial growth of the oyster mushroom. The concentrations 25, 50, 100% of the leaky innate showed ability Tdhadah high - fungal plant pathogen (Aspergillus niger, Aspergillus flavus, Fusarium solani, Stymphllium sp., Penicillium sp.) Antigonsim when studying the negative ability of the fungus. The results showed a significant increasing in the growth standards and gaining characteristics of the oyster mushroom in the mass production of fruiting bodies called the gaining total according to the wet weight and it was higher production on corn husks and pomace dates 761.89, 735.13 g /kg of media and concentration of molasses 6%. when measuring efficiency of the fungus to product the largest amount of fruiting bodies observed that the middle pea - corn husks and pomace dates gave a larger amount of the remaining media and a concentration of 6% 76.18, 73.51%, have been affected by the weights of pods fungal change is another factor which affected by rates of molasses. The results were closely between nutrition media but nevertheless observed superiority of Corn husks and Pomace dates on the rest of media (15.04, 13.77)by using 6% concentration of molasses. when calculate the lengths of the necks of the body fruiting because of the great importance in a statement recipes - gaining produced and influenced by user medai - aligned and concentrations of molasses, as there were differences moral at all concentrations of molasses, when calculating the percentage of dry matter increased significantly increase the concentration of molasses and all concentrations compared with a comparison of strew wheat, which amounted to 12.16% when adding 2% molasses observed the emergence of highly significant differences between treatments and compared with control treatment, which gave treatment of Pomace dates highest rates, followed by corn husks and corn embryo and Al - gluten and Baggaz 8.21, 9.00, 9.28, 9.29%, respectively. For the purpose of obtaining material efficient in maintaining the objects fruiting intact throughout the storage period tested preservatives (such as Sorbic acid and black pepper) and concentrations 40.20g / l of articles in a row leads to prolong longevity reservoir simulation of the objects fruiting and maintain the quality characteristics of the objects fruiting. The results showed that the addition of material to the feeder molass medai AGRO of oyster mushroom had been increased the proportion of protein in the fruiting bodies, either with respect to calculate the proportion of carbohydrates had been found that the percentage of carbohydrates are associated with a high positive correlation relationship between the concentration of molasses and the different transactions that were used in the experiment. The oils were represented less than what can be had reached attribute to 0%. Well conducted safety checks the health of the mushroom oyster and the results were that the fungus free of toxins by anatomy organic to members liver, kidney and intestines and by the standards of blood physiological mice tested. And the importance of this project calculated the economic viability and profits and revenue obtained from such a project.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عبد الامير سمير سعدون
اسم المؤلف: سولاف حامد تيموز

تاثير الحمل في بعض وظائف الكبد وعدد من المتغيرات الدموية والكيموحيوية The Effect of Pregnancy on Some Liver Functions And Blood And Biochemical Changes for The Pregnant Women in Al - Alam

المستخلص: تناولت هذه الدراسة تاثير الحمل في وظائف الكبد وعدد من صفات الدم الكيموحيوية حيث تم جمع عينات دم من نساء حوامل خلال مراحل الحمل الثلاث ومن نساء متزوجات غير حوامل كمجموعة سيطرة، وجرت هذه الدراسة في مركز العلم الصحي للفترة من اب 2008 الى ك 1 2009 وبلغ مجموع | This study took the effects of pregnancy on liver functions and number of biochemical blood characteristics, in which blood samples were taken from pregnant women during the three trimesters of pregnancy and from non - pregnant married women as a control group, and this study was done in The Alam health centre from August 2008 to December 2009, and the number of pregnant women were (120) women while the non - pregnant married women were (40) women, there ages were between 15 - 40 years old. The results of this study showed increase in Body Mass Index for pregnant women with progress of pregnancy compared with the non - pregnant married women givingP value (P<0.01) and there was appearance of specific elevation in Fibrinogen concentration in blood and Serum Albumin and Cholesterol concentration and Triglyceride in pregnant women compared with the non - pregnant married women giving P value (P<0.01). Also the results showed specific decrease in Bilirubin during the three trimesters of pregnancy compared with the non - pregnant married women especially in the third trimester giving P value (P<0.01). Also the results showed specific elevation in liver enzymes represented by Alkaline phosphatase and Aspartate transaminase enzyme and Alanine transaminase enzyme especially in the last two trimesters of pregnancy in comparision with the non - pregnant married women giving P value (P<0.01), but for Malondialdehyde, the results showed specific elevation during the three trimesters of pregnancy compared with the non - pregnant married women in contrast to the Glutathione antioxidants that showed specific decrease during the three trimesters of pregnancy in comparasion with the non - pregnant married women giving P value (P<0.01). And the study showed also the effects of pregnancy on the blood elements, in which there was obviouse decrease in number of Platelets during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01). And the Heamoglubin concentration, Red Blood Cell number and the mean corpuscle Volume Suffer Specific decrease during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01).
الصفحات الاولى:
الكلمات الدلالية:
  • الحيوان - الفسلجة
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: صالح محمد رحيم العبيدي
اسم المؤلف: رؤى حسين علوان الجبوري

عزل وتشخيص بعض انواع بكتريا السالمونيلا من بعض الاغذية المحلية في مدينة تكريت Isolation And Identification of Some Species of Salmonella From Some Local Foods in Tikrit City

المستخلص: تم التحري عن بكتريا السالمونيلا في 520 عينة من الاغذية المحلية في مدينة تكريت, وجدت هذه البكتريا في 26 عينة (5%), كانت اعلى نسبة لتواجدها في عينات اللحوم المثرومة (2.5%) والتي تمثل 16.25% من العينات الكلية للحوم المثرومة المدروسة.عزلت السالمونيلا من عي | The presence of Salmonella spp. was investigated in 520 local food samples in Tikrit city, Salmonella was found in 26 samples (5%), with the highest presence in minced meat (2.5%) which was represented 16.25% of all minced meat samples. Salmonella was isolated from chicken's meat at a percentage of 3.75%, and same percentages for meat slices samples, egg cortex and chicken's blood represented 5% for each one, and isolated too from vegetables and cheese at a percentage of 2.5% for each one. No Salmonella has been detected from sweet, yoghurt and egg content. Six Salmonella species were isolated, S. typhimurium was to be the most frequent and represented 42.30% of positive samples, then S. enteritidis and S. anatum in 34.61% and 11.53% respectively, but S. agona, S. typhi and S. arizona existed in a percentage about 3.84% for each one. Also, all the isolated species showed their own serological formula. The selective medium Tetrathionate broth (TTB) was found to be superior for isolation of Salmonella than Selenite cystine broth (SCB), the former positivity was 96.2% and 88.5% respectively. For test of better selective medium for isolation, TTB was found to be more suitable for isolation of S. anatum, while SCB was better for isolation of S. arizona.All the six isolated Salmonella species showed high sensitivity to Ciprofloxacin (5?g) and moderately sensitive to Chloramphenicol (30?g), while they showed high resistance to three antibiotics Amoxicillin (30?g), Nalidixic acid (30?g) and Pipracillin (100?g). They showed resistance with different percentages to the other tested antibiotics : Ampicillin (10?g), Gentamicin (10?g), Streptomycin (10?g), Tetracycline (30?g) and Trimethoprim (25?g).
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: عبد الكريم فتاح عمر
اسم المؤلف: شيماء جابر حميد البياتي