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تاثير المستخلص الكحولي الخام لاوراق الجت (Medicago sativa) على بعض المعايير الفسلجية والكيموحيوية في اناث الجرذان المستحثه بمادة الالوكسان == Effects of Crude Alcoholic Extract of Alfalfa (Medicago Sativa) Leaves On Some Physiological And Biochemical Parameters In Alloxan Induced Diabetic Female Rats
Author name:
حوراء سليم محمد طه بشيبش
Supervisor name:
عبد الزهرة كاظم محمد علي
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Kufa - College Of Science - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
24T2801 - p.pdf
Abstract:
اجريت هذه الدراسة في مختبر البحوث السريرية في كلية الصيدلة ومختبر فسلجة الحيوان في كلية العلوم / جامعة الكوفة للمدة من 15 / 9 /2011 الى 12 /3 /2012 للتقصي عن فعالية المستخلص الكحولي الخام لاوراق نبات الجت Medicago sativa L. في تخفيض مستوى سكر الدم وبعض ال | This study was conducted at the Clinical researches laboratory/ Pharmacy College and Animal physiology laboratory/ Sciences College / university of Kufa during the period from 15/ 9/ 2011 to 12 / 3/ 2012 to investigate the activity of the crud alcoholic extract of the alfalfa (Medicago sativa L). leaves in affecting the blood glucose level and some physiological and biochemical changes due to induced diabetes mellitus by alloxan (150) mg/kg in female albino rats (Ratus ratus), in comparison with the activity of the reference drugs Glibenclamide. This study was also used to know the effect of the extract in normal (healthy) female rats. In this study (54) female rats were utilized which were divided into (9) groups with 6 rats for each group. Diabetes mellitus was induced in (30) female rats, were distributed into 5 sub groups. One of them was considered as control group for the infected rats, and three groups of them were treated with different concentrations of the extract (250, 500 and 750)mg/kg while the fifth group was treated by the chemical drug Glibenclamide (0.6) mg/kg. The other 24 normal rats were also divided into 4 sub groups, one of them considered as control group. And the other three groups were treated with the extract concentrations (250, 500 and 750)mg/kg. The treating period with the plant extract and the drug continued for 30 day.Results of this study were as the following : ? A significant (P< 0.05) decrease was showen in total body weight, insulin hormone and HDL - c levels, total WBC and RBC count in addition to Hb concentration in infected group of diabetes mellitus in comparison with healthy control group.? A significant (P< 0.05) increase was clear in the levels of glucose, TC, TG, LDL - c, VLDL - c, urea, creatinine and ALT and AST enzymes in infected group in comparison with healthy control group.? The healthy rats that treated with the extract concentrations were showed a significant increase in their total body weight at the end period as compared with the first day of experiment, there was also a significant (P < 0.05) increase in body weights of the infected rats that were treated by the two concentrations (500, 750) mg/kg of extract as compared with infected control group.? The infected rats that treated by all three concentration of plant extract and the glibenclamid drug were exhibited a significant (P< 0.05) increase in insulin level as compared with infected control group.? on the other hand, it was also showen a significant (P< 0.05) decrease in glucose level of infected rats that have been treated by the three concentrations and the (0.6) mg/kg concentration of glibenclamid as compared with infected control group.? The levels of TC, TG, LDL - c, VLDL - c were significantly (P< 0.05) decreased, in contrast a significant increase was occurred in the level of HDL - c in infected rats when they treated by all the concentrations of plant extract as compared with infected control group.? The urea and creatinine levels showed a significant (P< 0.05) decrease when the infected rats were treated by the two concentrations (500, 750) mg/kg as compared with infected control group, while in the healthy rats, the urea level showed a significant (P< 0.05) decrease as compared with healthy control group.? A significant (P < 0.05) decrease was occurred in ALT enzyme level when the infected rats were treated by all extract concentrations and the chemical drug. But AST enzyme level was significantly (P < 0.05) decrease when the infected rats were only treated with the two concentrations (500, 750) mg/kg of plant extract in addition to glibenclamid as compared with healthy control group.? The two concentrations (500, 750) mg/kg of plant extract and the concentration (0.6) mg/kg of the glibenclamid drug caused a significant (P< 0.05) increase in total RBC count, and the hemoglobin concentration, while only the larger dose of plant extract caused a significant (P < 0.05) increase in total WBC count in infected rats as as compared with infected control group.In conclusion, the results of this study indicate that the two concentrations (500, 750) mg/kg of alfalfa leaves extract gave a good results, even better than the reference drug, glibenclamid for treating diabetes mellitus, in addition to ameliorate the metabolic disorders that accompany it.
