تعيين بعض الظروف الزرعية لانتاج انزيم السليليز والكحول الاثيلي من بعض انواع البكتريا المعزولة من التربة == Determination of Some Cultural Conditions For Cellulase And Ethanol Production By Bacteria Isolated From Soil
Author name:
عبد الستار عبد الجبار ابراهيم
Supervisor name:
عدنان نعمة عبد الرضا العزاوي | انيس عبد الله كاظم
General topic:
Biology
Specific topic:
Microbiology - Enzymes
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Diyala
First pages:
24T2752 - p.pdf
Abstract:
The study included isolate and diagnose of some types of anaerobic and aerobic bacteria capable of decomposing and fermentation of cellulose. Isolation and diagnose the bacteria Clostridium phytofermentous from 10 agricultural soil samples, was 50 % of the total 50 colony, while isolation of Escherichia coli from 15 waste water samples which represented 40% of the total 75 colony and isolated Pseudomonas aeruginosa from 15 normal soil samples, and was 53% of the total 75 colony. production of cellulase enzymes was evaluated by using absorbance method. Higher activity obtained via Clostridium phytofermentous with maximum value of 42.8 IU / mL, while Pseudomonas aeruginosa gives 12.5 IU / mL and Escherichia coli gives 29.1 IU / mL.. Optimum temperature was 35 C and pH 7, for production cellulose enzyme The concentration of ethanol Alcohol was measured by using Titration method. Maximum concentration of ethanol alcohol produced by Escherichia coli, which reached 8.28 g/liter, while Pseudomonas aeruginosa was 7.86 g/liter, and Clostridium phytofermentous reached 6.62 g/L.
