مستوى الهستامين وبعض المعايير المناعية لمرضى الحساسية التنفسية من النوع الاول == The Level of Histamine And Some Immunological Parameters Among Airway Hypersensitivity Type I Patients
Author name:
سلوى احمد حمزة الكرادي
Supervisor name:
فريال جميل عبد
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Babylon - College Of Science - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T2718 - p.pdf
Abstract:
تم جمع 50 عينة دم من مرضى الحساسية التنفسية من النوع الاول المراجعين لمركز الحساسية والربو في محافظة بابل للفترة الممتدة ما بين تشرين الثاني 2013 ولغاية شباط 2014, بالاضافة الى 10 عينات دم من الاشخاص غير المصابين بالحساسية من النوع الاول والذين كان معدل I | Blood samples have been drawn from 50 airway hypersensitivity type I patients who visit the center of the allergies and asthma in the province of Babylon, for the period between November 2013 and up to February 2014, in addition to 10 samples from persons with normal IgE level as a control. The sample was divided into two parts, the first used to measure the level of eosinophils and basophils and the second used to measure the level of immune parameters, which included histamine, IgE, IL - 5, IL - 10 and IL - 12 by using the technique of Enzyme - Linked Immunosorbant Assay (ELISA). Study community were divided into three groups, the first group named before immunotherapy treatment and the second group after immunotherapy treatment and final is for control. Conducted differences in the level of immune parameters between the three groups, there were significant differences between the group before the treatment and control group as well as between the group before treatment and after treatment. The level of histamine for the three groups (before treatment and after the treatment and control), reached respectively 34.8292 ± 6.47680 ng / ml and 9.3238 ± 3.55296 ng / ml and 6.9844 ± 3.05477 ng / ml, and the level of IgE reached to the before treatment group 40.6279 ± 15.24231 IU / ml and the after treatment group 23.9529 ± 11.88933 IU / ml and control group 11.7796 ± 5.53 IU / ml, while the cell count of eosinophils for the before treatment group 0.7283 ? 0.24556 x 103 cells / mm 3 and after treatment group 0.3643 ? 0.14861 x 103 cells / mm 3 and control 0.2252 ? 0.09139 x 103 cells / mm 3 , as well as for the cells of basophils for the before treatment group 0.1008 ? 0.03391 x 103 cells / mm 3 and after treatment group 0.0777 ? 0.03485 x 103 cells / mm 3 and control 0.0724 ? 0.03568 x 103 cells / mm 3 , As for the cytokines the level of IL - 5 reached for the same groups 51.3214 ± 6.18565 pg / ml and 22.7254 ± 3.99360 pg / ml and 24.5404 ± 8.64920 pg / ml respectively, while IL - 10 has reached 14.9604 ± 6.48170 pg / ml and 43.0476 ± 21.26668 pg / ml and 24.4969 ± 10.21066 pg / ml, respectively, and finally the level of IL - 12 reached for the same groups 17.753 ± 8.39343 pg / ml and 39.1929 ± 17.73975 pg / ml and 26.7731 ± 12.93092 pg / ml, respectively. There were been a difference in the level of immune parameters for a number of variables, , place of residence, gender, and type of allergy, there were no significant differences observed in the level of immune parameters between these variables. comparison was made in the level of immune parameters for the group after the treatment depending on the duration of immunotherapy, and found that there were significant differences in the level of these parameters with the increase of the duration of treatment. Through this study, conducted the correlation among histamine and the rest of the immune parameters and the results were pointing to the positive correlation of histamine with IgE, IL - 5, eosinophils and basophils and negative correlation with IL - 10 and IL - 12.
