التحري عن جينات المقاومة لمضادات البيتالاكتام واسعة الطيف في بكتريا Acinetobacter baumannii المعزولة من مصادر سريرية == Detection of Extended Spectrum Beta - Lactam Resistance Genes In Acinetobacter Baumannii Isolated From Clinical Sources
Author name:
صفا ماجد محمد الباجلاني
Supervisor name:
عباس عبود فرحان الدليمي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Diyala
First pages:
24T2776 - p.pdf
Abstract:
تضمنت الدراسة عزل وتشخيص 16عزلة من بكتريا Acinetobacter baumannii من اصل 196 عينة جمعت من مصادر سريرية مختلفة في مستشفى بعقوبة العام ومستشفى البتول التعليمي وكانت اعلى نسبة عزل لهذه البكتريا من مسحات الجروح %10.8، ومسحات الحروق بنسبة 8.3%، والادرار 6.9%، | The study included isolation and diagnosis of 16 isolates of Acinetobacter baumannii out of 196 samples collected from different clinical sources in Baquba General Hospital and Al - Batool Teaching Hospital. The highest rate for the isolation of these bacteria from wounds 10.8%, burns 8.3%, urine 6.9%, and blood 5%. The diagnosis of isolates was confirmed by ViTEK2 device in addition to phenotypic, microscopic characteristics and biochemical tests.The results of the investigation of virulence factors for Acinetobacter baumannii isolates showed that all isolates have the ability to adhesion surfaces of epithelial cells of humans (100%), while the ability of 13 isolates to produce biofilm was 81.2%, seven isolates had possessed efflux pumps with high efficiency (43.7%). The investigation of the sensitivity of the isolates for ten antibiotics, the results showed all isolates were resistant to antibiotic cephalexin was 100%, against antibiotics meropenem and imipenem was 50%, the other antibiotics resistance ratios ranged between 81.2% - 56.2%. The results of multi - drugs - resistance tests showed ten isolates of Acinetobacter baumannii possessed of multiple antibiotic resistance, the isolates were divided into two groups, resistance group and other sensitive depending on the resistance to antibiotics, as the first group included ten isolates resistance from 6 - 9 antibiotics, the second group included six isolates resisted from 1 - 4 antibiotics, and the first group is dominant in the study. The concentration (100 µg/ml) of cephalexin, cefotaxime, ceftriaxone to test the ability of isolates were resistant to cephalosporin antibiotics, all isolates showed resistance to this concentration for antibiotic Cephalexin, the resistance Cefotaxime, Ceftriaxone to this concentration was 75% for both antibiotics. The combination disk method used to investigate isolates producing for extended spectrum ? - lactamase enzyms and metallo ? - lactamase enzyms the results indicated that the percentage of isolates under study producing extended spectrum ? - lactamase enzymes and metallo ? - lactamase were 62.5% and 56.2% respectively from the total 16 isolation. It was determined the minimum inhibitory concentration MIC for cefotaxime and ceftazidime by method of multiplying serial concentrations, The results showed that the minimum inhibitory concentration for cefotaxime values ranging between 32 - 1024 µg/ml, as for the MIC ceftazidime his values ranging between 16 - 1024 µg/ml. Conducted process extract bacterial DNA for isolates of Acinetobacter baumannii, then held polymerize chain reaction PCR for isolates resistant to antibiotics ? - lactam and MIC values more than 64 µg/ml through the use of specialized primers that target specific sequence of genes blaCTX - m and blaSHV, relay outputs of the multiplication agarose gel concentration of 1%, the results showed that 5 isolates of Acinetobacter baumannii out of 10 isolates possessed gene blaCTX - m and by 50%, as for blaSHV gene the results showed that three of the isolates under study only has this gene and by 30%.
