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تاثير نقص البروتين في بعض التغيرات النسجية والسريرية والوظيفية في ذكور الفئران البيض == The Effect of Protein Deficiency In Some Clinical, Histological And Physiological Changes In Male White Mice
Author name:
نسم عماد دايم الفؤادي
Supervisor name:
فاضل فرهود مكي الجبوري | هاشم محمد عبد الكريم العلاق
General topic:
Biology
Specific topic:
Life Science
Degree:
Master
University:
University of Babylon - College Of Science For Girls - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T2778 - p.pdf
Abstract:
The present study is designed to know the effect of protein deficiency on mice males through study the clinical histological changes of some organs including (liver, kidney, stomach and testes).In addition of study the blood and biochemical parameters, where they were sheltering animals in the animal house of the branch of physiology / College of Veterinary Medicine / University of AL - Qadisiya and the number was 90, all of the mice are males and age (28 - 30) days kept for two weeks before starting the experiment for the purpose of acclimatization was given during this period, diet control, then the experiment began between 01/05/2014 until 19/2 / 2014, and at weights ranging from start racing experience between 17 - 18 g, all in a period of growth.These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different period as follow (15, 30, 45)day.Experiment takes study of changes in body weight of animals and some selected organs also study histological changes for studied organs, also physiological blood parameters such as PCV, Hb, GCS and TSP.Results showed significant decrease (p<0.05) in weight body rate of animals, also in the rate of selected organs means compared with control group. The second treatment for 45 days most significant in decreased of body weight and organs weight if it compared with first treatment in all periodsHistological procedures showed that clinical and histological changes are graduated by the severe of protein deficiency and period of time. The histology investigation for liver showed that congestion of blood vessels accompanied with dilation of central vein and portal vein, and necrosis of the hepatic cells and destroyed the general total structure of liver. The section of kidney showed that necrosis and destroyed in renal tubules and epithelial cells in the lumen of tubules and bleeding in renal tubules and clear shrinkage in glomerulus. There are histological changes in stomach by necrosis and desquamation for gastric cells in both period (30, 45) days, while there are no histological clinical changes in both doses in period (15 days) for exposure. Histological sections of testes showed induction inhibition of spermatogenesis and desquamation of germ cells and congestion in the interstitial tissue and dilation of lumen of seminiferous tubules in both periods (30, 45) days, while there is no any changes in both first and second treatment in period of (15)days.In contrast, the hematological parameters showed that the concentration of Hb, PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.Biochemical tests obtain significant decrease (p<0.05) in blood glucose level in both treatment first and second in first period. Also the results showed that no significant increase in first treatment in both periods (30, 45) days compared with control group, while there is significant increase in the level of blood glucose in the second treatment in both periods (30, 45) days compared with the control group. Also did not score a significant decrease (P <0.05) in the total serum protein in thefirst treatment in the first period (15) days, compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while found a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group.It can be conclusion that the exposure for protein deficiency due to clear clinical histological changes for studied organs and some changes in some hematological and biochemical parameters in white mice which treated with protein deficiency which it causes these symptoms
