Share
عوامل الخطورة لطفرات الجينين CBS وMTHFR لقبل الارتعاج لدى النساء الحوامل في النجف == Risk Factors of CBS And MTHFR Gene Mutation For Preeclampsia In Pregnant Women In Al - Najaf
Author name:
زهراء سامي رزاق النجار
Supervisor name:
ظافرة جعفر عبد علي الفتلاوي | ماجد كاظم حسين
General topic:
Biology
Specific topic:
Zoology
Degree:
Master
University:
University of Kufa - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
24T2789 - p.pdf
Abstract:
تعد الاصابة بقبل الارتعاجPre - eclampsia من الاعتلالات الخطيرة التي قد تظهر في الثلث الثالث من مدة الحمل اذ تتصف هذه الاصابة بارتفاع ضغط الدم وظهور بروتين الالبومين في الادرار. وقد ظلت اسباب نشوء هذه الحالة غير واضحة المعالم, اذ اعزيت بعض من اسبابها الى ا | Preeclampsia is considered as one of the serious complication in the third trimester of pregnancy. It is associated with hypertension and proteinuria. The causes of preeclamptic are still not well understood. Evidences suggested the involvement of metabolic related mutations in the disease. In the current study, the genetic mutations of CBS and MTHFR genes are studied as markers for the diagnosis of preeclampsia. Sixty preeclamptic pregnant women and forty healthy pregnant women as control group diagnosed by the physicians at Azzahra Teaching hospital for obstetrics &pediatrics in Najaf province from December 2012 to August 2013 The poly merase chain reactions technique was used to evaluate the incidence and frequency of C?S &MTHFR mutations. A questionnaire was conducted to obtain the information of the patients and the control group. Genetic mutation were detected by polymerase chain reaction (PCR)and agaros gel electrophoresis. The information indicated that the age of pregnant women, gestation age, previous history of the patients, family history and parity are involved in the pathogenesis of preeclampsia. The results of the present study of preeclampsia showed a significant increase (P ? 0.01) in patients (25 - 34) years old when compared with the other group. where the results found a significant increase (P <0.01) of Preeclampsia occurred during (33 - 39) weeks of pregnancy. And in the patients with previous history for preeclampsia there was significant increase (P<0.001) compared with healthy pregnants. Pregnants with preeclampsia in the family history showed significant increase (P< 0.01) as for women pregnant for the first time showed and significant (P<0.01) and constituted 8% pregnanst with preeclampsia who had diabetes mellitus. The extraction and quantification of cell free fetal DNA (cff DNA) revealed a significant (P<0.0001)increase in preeclamptic patients when compared with those of healthy pregnant women. It is confirmed by the use of an internal negative control through the monitoring of SRY gene (Y chromosome gene) amplification that the gene belong to the infant and 40 pregnant woman were followed up till the labor. Thus, 9 of them had delivered males, while 30 had delivered females. The wild type SRY gene band was obtained clearly from those of male delivery, but not from those of female delivery. Mutations of C?S gene were analyzed by theuse a primer intended to amplify a fragment of171 bp.The rate of mutation was found to be 51.7% and 5% in preeclamptic patients and healthy pregnant women respectively and Odd Ratio=20.31, P value=10 - 7. The C677T mutation of MTHFR gene was verified by the use of a primer designed to amplify a fragment of 198bp. The prevalence of mutation was indicated to be 68.3% and 17.5% in the preeclamptic patients and healthy pregnant women respectively and Odd Ratio =10.17, Pvalue =6.2*10 - 7. Results demonstrated the wide involvement of C?S and MTHFR gene mutation in the pathogenesis of preeclampsia, but they are no crucial for the development of the disease. In addition there are so many factors thatmay be involved in such development.
