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تقييم مستوى بعض الحركيات الخلوية لدى المرضــى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1 == Evaluation of The Level of Some Cytokines In Patients With Herpes Simplex Virus Type I (HSV - 1)
Author name:
حسين علي كاظم
Supervisor name:
سهام جاسم الكعبي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
24T2796 - p.pdf
Abstract:
هدفت الدراسة الحالية الى تسليط الضوء على مجموعة من المرضى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1(HSV - 1) من خلال تقييم مستوى نوعين من الحركيات الخلوية الموجودة في مصولهم. شملت هذه الحركيات الخلوية الانترلوكين Interleukin - 6(I | The present study aimed to highlight a group of patients infected with herpes simplex virus type I (HSV - 1) by evaluating levels of two types cytokines in sera. These included cytokines interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - ?) has been evaluated using the technique of Enzyme - linked immunosorbent Assay (ELISA), has also been investigating the relationship between these cytokines and the stages of infection compared to the control group. Before that rapid cytologic method has been used to diagnose the virus by detecting the presence of multinucleated giant cells, and then was diagnosed serologically by specific IgM and IgG monoclonal antibodies against Herpes simplex virus - 1. It was also used Radial immunodiffusion method to evaluate levels of total IgM and IgG in patients sera.Sixty four sera samples were collected from patients who are attending to the consultants clinics of Al - Hakeem general hospital and AL - Sader teaching hospital in AL - Najaf AL - Ashraf governorate for the period from October 21/2012 until March 13/2013. These Samples were divided into two groups : (32) sample during primary and recurrent infection, (32) after recovery from infection (latent stage), as well as (16) sample were collected from healthy individuals, who were regarded as acontrol groups.The study reached to the following results : • A significant increment (p<0.05) in sera concentrations of IL - 6 in patients during infection as compared with control groups, but the increment was not significant in sera of patients during latent stage.• TNF - ? levels increased in sera of patient, but this increment was not significant as compared with controls group.• There was no significant increase of Total IgM levels in sera of patients.• Total IgG levels increased significantly in sera of patients during infection and latent stage as compared with control group.• A highly significant positive correlation (r= 0.7, p= 0.00) between TNF - ? and IL - 6 concentration in sera of patients.• There was no significant correlation (p>0.05) between Total IgM levels and (IL - 6 and TNF - ?) in sera of patients with correlation coefficient (r= 0.1, r=0.2) respectively.• Positive significant correlation (r= 0.4, p= 0.02) was found between total IgG levels and IL - 6 in patients during infection, but was no correlation between them in latent stage.• There was no association between age and IL - 6 and TNF - ? levels in sera of patients with HSV - 1.Finally, the elevation of TNF - ? and IL - 6 levels in sera of infected patients with HSV - 1 may be play an important role in pathogenicity of virus, as well as it may limits the viral infection.Furthermore, there are positive correlation between levels of TNF - ? and IL - 6 in sera of patients
