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ضغط البيانات باستخدام تمثيل الترميز التسلسلي == Data Compression By Using Chain Code Representation

Author name: فنر علي جودة
Supervisor name: توفيق عبد الخالق الاسدي
General topic: Computer Science
Specific topic: Computer Science
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: In this thesis we have proposed an efficient lossless image compression system. This system consists of two parts : the first part is compression procedure that is based on two approaches of chain code representation. The first approach is Variable Vertex

اكتشاف العيب للصور الشعاعية مع التعليق باستخدام المربع الاصغر لماكنة الدعم الموجه وقواعد عامة وخاصة للتصنيف == Defect Detection of Radiography Image With Annotation Using Lssvm_Gsc Techniques

Author name: وفاء محمد سعيد الاسدي
Supervisor name: يحيى مهدي هادي الميالي
General topic: Computer Science
Specific topic: Computer Science
Degree: Doctorate
Language: English
University location: Babylon
Key words:
  • Image Processing
  • Weld Image Defect Detection
  • Image annotation
  • Radiography Image
First pages:
Abstract: استرشادا بالشعوربالمسؤولية ورغبة في مساعدة اكتساب معرفة عميقة حول دراسة تنوع العيوب في صور التصوير الشعاعي، لهذا سنتعرف على هذه العيوب الموجودة في هذه الصور، ومن ثم اعطاء كل صورة بعض الكلمات التي تعكس محتوياتها الرئيسية، وبناء قاعدة بيانات للصور مع الشرح | Guided by the sense of responsibility and its desire to help gain a deep knowledge and the diversity of the defects exist in the radiography images, this Dissertation recognizes the defects exist in the these images, then given each image some keywords re

نمذجة مقتزح لتحسين تنبؤ استيقاظ متوسط التحكم بالوصول في شبكات الاستشعار اللاسلكيه == Modeling A Suggested Modified Predictive Wakeup Medium Access Control on WSN

Author name: فوزية وليد خزعل
Supervisor name: سعد طالب حسون الجبوري
General topic: Computer Science
Specific topic: Computer Science
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: The deployment of the wireless sensor networks (WSN) was increased due to the rapid development in all electronic and communication devices. There are many problems and challenges regarding their applications. One of their major challenges is the energy p

تصميم وبناء نظام هجين للتنبؤ بالشكل الثلاثي للبروتينات == Design And Implementation of Hybrid System For Protein Tertiary Structure Prediction

Author name: مهند محمد جاسم الياسري
Supervisor name: نبیل ھاشم الاعرجي
General topic: Computer Science
Specific topic: Computer Science
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: تبقى مشكلة التنبؤ ببنية البروتين كنوع من التحدي العقلي والتنفيذي على حد سواء. (PSP) تبقى مشكلة التنبؤ ببنية البروتين وبالتالي فان اهداف هذه الاطروحة هي : اولا، اقترح منظورا جديدا للمساهمة في تطوير وزيادة المعرفة في مجال "طي البروتين العملية" (عن طريق وصف | The Protein Structure Prediction (PSP) problem remains as both mental and implemental challenge. Therefore, the objectives of this dissertation are : First, suggest new perspective for contributing in development and knowledge increasing in the "protein f

تقنية توليد مفتاح منحنيات بزير بالاعتماد على بصمة الاصبع لامنية المعلومات == Bezier Curves Key Generation Based on Fingerprint For Secure Information

Author name: وائل مهدي بريج
Supervisor name: وسام سمير بهية
General topic: Computer Science
Specific topic: Information Technology
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: المقاييس الحيويه هي احد الحقول المهمه في تعريف هوية الانسان والامنيه. بصمة الاصبع فريده لكل شخص وهي لاتتغير خلال الحياة. تقترح هذه الاطروحه تقنيه جديده في امن المعلومات والتثبت من هوية المرسل من خلال توليد مفتاح تشفير من بصمة الاصبع. يعتمد المفتاح المتولد | Biometrics is one of the important tendencies in human identification and security. Fingerprint is unique for any individual and it is not change through the life. This thesis suggests an approach for information secrecy and authentication on networks. T

تقويم امنية البلوتوث باستخدام تقنية المنطق المضبب == Evaluation of Bluetooth Security Based on Fuzzy Logic Technique

Author name: سمير حميد عبد الشهيد
Supervisor name: ستار بدر سدخان المالكي
General topic: Computer Science
Specific topic: Computer Science
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: خوارزمية تشفير البلوتوث E0 هي نوع من انظمة التشفير الانسيابي، مع امنية جيدة، والتي تستخدم على نطاق واسع في نظام الاتصالات اللاسلكية. الهدف الرئيسي لهذه الرسالة هو استخدام المنطق المضبب لتصميم مقوم هما (E تم اختيار معلمتين ( يتعلقان بمتتابعة المفتاح الم | The Bluetooth E0 cryptosystem is a type of the stream cipher cryptosystem, with good security, and it is widely used in wireless communication systems.The main aim of this thesis is to use the Fuzzy Logic to design an evaluator for the security of Bluet

حول التوزيعات المختلطة == on Mixture Distributions

Author name: رسل ناصر عباس
Supervisor name: كريمة عبد الكاظم مخرب الخفاجي
General topic: Mathematics
Specific topic: Mathematics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: نظرا لاهمية التوزيعات الخليطة في مختلف مجالات الحياة ) الطب، الزراعة، الصناعة،.....( كانت مساهمتنا هذه من خلال هذا البحث في بناء التوزيع الثنائي الخليط حيث يعتمد على خلط هذا التوزيع مع توزيعات Possion, Erlang, wighted lindly على تحويل المعلمة. باسثخدام | Due to the importance of mixture distributions and mixture models, which are statistical models involving mixture distribution, in many fields of real life like biology, chemistry, geology, medicine, actuarial science and so on, so our contribution is to

حول التقريب الرتيب للدول متعددة المتغيرات == on Bivariate Monotone Approximation

Author name: جنان محمد هادي القيسي
Supervisor name: ايمان سمير عبد علي بهية
General topic: Mathematics
Specific topic: Mathematics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: This thesis deal with the two dimensional problem for monotone approximation.we answer George A. Anastassiou question and we generalize a direct theorem for monotone approximation to the two dimensional theorem for monotone approximation to function in f

الخواص الدينمية لدالة ايكيدا == The Dynamical Properties of Ikeda Map

Author name: وفاء هادي عبد الصاحب
Supervisor name: افتخار مضر طالب الشرع
General topic: Mathematics
Specific topic: Mathematics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: في عملنا, درسنا داله ايكيدا وهي من الدوال الغير خطيه في البعد الثالث وصيغتها I_(a,b) ((x@y@t))=(1+a x cost - a y sint@a x sint+a y cos?t@bt)) اعداد حقيقيه a,b حيث درسنا الخواص الدينمية العامه لداله ايكيدا ثم درسنا الخواص الفوضويه لها حيث برهنا ان داله ايك

تاثير اضافة النحاس والخارصين على بعض الخصائص الفيزيائية لاغشية اوكسيد النيكل النانوية المرسبة بطريقة المحلول الغروي == Effect of Additive Copper And Znic on Some Physical Properties of Nano Nickel Oxide Films Deposited By Sol - Gel Method

Author name: نور علي سامي الجيلاوي
Supervisor name: فؤاد شاكر هاشم الجبوري
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: حضرت نانوية NiO)) النقية والمضاف لها الخارصين والنحاس بنسب مولية (0.01,0.05,0.1) mol% ومزيجهما بالنسبة 0.1mol%عند سمك 100 nm على بابعاد 1mm)× 1.5×2) باستخدام طريقة المحلول الغروي Sol - Gel)) عند درجة حرارة الغرفة (R.T). درست الخصائص التركيبية للاغشية ا | NiO : Zn,Cu nano films were prepared by molar percentage of (0.01,0.05,0.1) mol% and (0.1) mol% mixture under 100 nm thickness onto (2 ×1.5×1) mm glass surfaces by Sol - Gel method at 298K.The structure properties for the prepared films were studied the

تاثير قطر وشكل فتحة المسدد على طيف الطاقة لنضيري 137Cs و22Na باستخدام الكاشف الوميضي NaI(Tl) == The Effect of The Diameter And The Shape of The Collimator In The Gamma Ray Energy Spectrum For 137Cs And 22Na Isotopes Using Scintillation Detector NaI(Tl)

Author name: مالك صالح محيميد الحمداني
Supervisor name: خالد حسين هاتف العطية
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تناول هذا البحث دراسة تاثير قطر فتحة المسدد وشكله على طيف الطاقة للنظيرين المشعين (Cs - 137) و(Na - 22) وذلك باستخدام مسددات من مادة الرصاص ذات فتحات في مراكزها باقطار مختلفة, اذ تم اجراء الدراسة باستخدام الكاشف الوميضي [NaI(Tl)] ومنظومة العد والتحليل الا | In the present work, the effect of the diameter, the shape and the position of the collimator in the gamma ray energy spectrum have been studied by using scintillation detector NaI(Tl) of sizes (1 x 1.5) inch and the nuclear counting and analysis system t

امثلية العدسات المغناطيسية ثنائية القطب المتناظرة == Optimization of Symmetrical Double Polepiece Magnetic Lenses

Author name: لمياء ميري صالح الجنابي
Supervisor name: طالب محسن عباس الشافعي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: A computational study has been carried out to investigate the influence of the optimization variables, by mathematical function represent axial magnetic field distribution for magnetic unsaturated double polepiece magnetic lens. It should be mentioning th

تحضير ودراسة بعض الخصائص الفيزيائية لمادة متراكبة من راتنج الايبوكسي المقوى بالرماد المتطاير وغبار السيليكا == Preparing And Studying Some Physical Properties of Composite Material Which Reinforced With Fly Ash And Silica Fume

Author name: محمد باسم غفار الجبوري
Supervisor name: نهاد عبد الامير صالح المعموري | محمد حمزة المعموري
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تتكون المواد المركبة من دمج مادتين او اكثر مختلفتين في الخواص الميكانيكية والفيزيائية ينتج عن ذلك مادة جديدة ذات خواص هندسية وفيزيائية تختلف عن خواص المواد الداخلة في تركيبها. ان اهم المواد المستخدمة في هذا البحث هو راتنج الايبوكسي مادة كيمائية تعتبر احد | Composite material consists of merging two composite materials or more are different in mechanical and physical properties resulted in a new substance has new different properties differ from the properties of the composing materials. Most important mater

دراسة الخواص الكهربائية للمركبات النانوية (PMMA - Ag) و(PMMA - Ti) == Study of The Electrical Properties of (PMMA - Ag) And (PMMA - Ti) Nanocomposites

Author name: فاروق ممتاز عبد عمران الجبوري
Supervisor name: بهاء حسين صالح ربيع المرعب
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: Two types of nanocomposites (PMMA - Ag) and (PMMA - Ti) have been prepared using casting method and different concentrations of nanomaterial (silver nanoparticles (Ag), titanium nanoparticles (Ti)). The electrical properties (D.C and A.C) of the (PMMA - A

حسابات طريقة دالة الكثافة لانظمة جزيئية واهب جسر قابل == Density Functional Theory Calculations For Donor - Bridge - Acceptor Molecular Systems

Author name: جعفر طعمة احمد
Supervisor name: حامد ابراهيم عبود محمد التميمي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:

تاثير اضافة (الفضة, السيريوم) على بعض الخصائص الميكانيكية والتركيبية لسبيكة (المنيوم - سليكون) == The Effect of (Ag, Ce) Addition on Some Mechanical And Structural Properties For (Al - Si) Alloy

Author name: الحاق عبد مسلم حسن صكبان
Supervisor name: عبد العزيز عبيد موسى العكيلي | جاسم محمد سلمان المرشدي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: تعد سبائك الالمنيوم المسبوكة مع السليكون كعنصر سبك رئيس من اهم السبائك التجارية بسبب امتلاكها خصائص سبك فائقة, ولهذه السبيكة تطبيقات واسعة في صناعة السيا ا رت والفضاء. يهدف هذا البحث الى دارسة تاثير اضافة عنصري الفضة والسيريوم على سبيكة )المنيوم_سليكون(, | Aluminum alloys with silicon as the major alloying elements are the most important commercial casting alloys because of their superior casting characteristics, which are widely applied in the aerospace and automotive industries.This work aims to study th

تاثير اضافة غشاء الالمنيوم الرقيق على بعض الخصائص الفيزيائية لمتراكب PVA - Ag النانوي == Effect of Addition of Al Thin Film on Some Physical Properties of (PVA - Ag) Nano - Composite

Author name: دلال حسن عبد الكاظم الشمري
Supervisor name: بهاء حسين صالح ربيع المرعب
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تم تحضير متراكبات نانوية (PVA - Ag) وبتراكيز مختلفة باستخدام طريقة الصب Casting method على الغشاء المعدني لمادة الالمنيوم Al) ) المحضربطريقة الترذيذ بالبلازما بسمك 150 nm) ) وتحت ضغط مقداره (2×10 - 1 mbar/pa) وفي نفس الوقت تم تحضير متراكبات نانوية ((PVA - | Nanocomposite (PVA - Ag) were prepared with different concentration by using casting the method of casting on the film material of Aluminum metal, prepared by plasma sputtering with thickness of (150 nm) and under pressure of (2×10 - 1mbar/pa). and has be

تخمين عامل زيادة خطر سرطان الرئة بسبب غاز الرادون في بعض مناطق محافظة بابل == Estimating Excess of Lung Risk Factor of Radon Gas In Some The Areas of The Babylon Governments

Author name: دعاء عبد الرزاق عاكول جاسم
Supervisor name: نهاد عبد الامير صالح المعموري
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: الرادون غاز مشع طبيعيا ينتج من التفكك الاشعاعي لعنصر الراديوم المشع طبيعيا وتنبعث منه جسيمات الفا حيث يعتبر الرادون المصدر الرئيسي للخلفية الاشعاعية الطبيعية حيث يساهم بحوالي اكثر من 60% من الجرعة الاشعاعية السنوية التي يتعرض لها الانسان على وجه الارض. ك | Naturally occurring radioactive radon gas is produced from radioactive decay of naturally radioactive element radium and emits alpha particles, where radon is the main source of natural background radiation contributes about where more than 60% of the ann

دراسة بعض الخصائص الفيزيائية لمتراكبات (PVA - PAAm - CoO) النانوية == Study of Some Physical Properties For (PVA - PAAm - CoO) Nanocomposites

Author name: حيدر محمد محسن التميمي
Supervisor name: مجيد علي حبيب | رحيم كعيد كاظم
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: البحث يتضمن تحضير المت ا ركبات النانوية (PVA - PAAm - CoO) باستخدام طريقة الصب ركيز مختمفة من جسيمات اوكسيد الكوبمت النانوية المضافة. وقد درست بعض الخصائص الفيزيائية قبل الاضافة وبعدها.اظهرت نتائج المجهر الالكحروو الماسح ) SEM ( وجحى ل فىر ر للاشعة جح | The research includes preparation of (PVA - PAAm - CoO) nanocomposites by using casting technique with different concentration of nano additives (cobalt oxide nanoparticles) and study of some physical properties of (PVA - PAAm - CoO) nanocomposites films

تاثير زيادة الجرع الاشعاعية لاشعة كاما على بعض الخصائص الفيزيائية لبوليمر كاربوكسي مثيل سليلوز == Effect of Increasing Gamma Ray Doses on Some Physical Properties of Carboxymethyl Cellulose

Author name: رسل عبد الامير غازي
Supervisor name: عبد الكريم جواد البيرماني
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تــــــم فــي هـــــذا البـــحث دراســة بعض الخـصائص الفيزيائية لبوليمر كاربوكسي مثيل سليلوز المذاب في الماء المقطر قبل وبعد تشعيعه باشعة كاما باستخدام المصدر المشع (Co60) ذي معدل طاقة مقدارها (6MeV) ومعدل الاشعاع المسلط ( (200 rad/minولفترات زمنية مختلفة | Some of physical properties of Carboxymethyl cellulose dissolves in distilled water had been studied before and after irradiation by Gamma ray using the source (Co60) of energy (6MeV) at rate (200 rad/min) for different times (25,35,45 minutes) at doses o

دراسة الخواص البصرية للعدسات المغناطيسية المزدوجة باستخدام طريقة التوليف == Study The Optical Properties of Doublet Magnetic Lenses By Using Synthesis Method

Author name: سارة معين شهاب المحنا
Supervisor name: طالب محسن عباس الشافعي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تم في هذا البحث دراسة الخواص البصرية المسقطية للعدسات الالكترونية المغناطيسية المزدوجة عديمة الدوران والتشويه باستخدام طريقة التوليف الامثل، ونقطة الشروع في هذه الدراسة استخدام دالة رياضية تحليلية لتمثيل المجال المغناطيسي المحوري لعدسة مغناطيسية منفردة(ث | In this research, the investigation has been introduced to study the projector optical properties of rotation and distortion free doublet magnetic electron lenses using optimum synthesis method.The starting point of this investigation is using analysis m

تحضير مادة مطاطية مركبة لتصنيع مانعات تسرب الزيوت == Preparation of Rubber Composite Material Used For Manufacture of Oil Seals

Author name: سعد عباس جاسم الجبوري
Supervisor name: نهاد عبد الامير صالح المعموري | محمد حمزة المعموري
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تم في ىذا البحث تحضير عجنة مطاطية مركبة لكي يتم استعماليا في تصنيع مانعات تسرب الزيوت وقد جرت الد ا رسة عمى اربع محاور الاول تضمن اختيار مطاط الاكريمونتريل بيوتادايين ) (NBR كمادة اساس تم في بادئ الامر اضافة مادة اسود الكاربون الييا وبنسب تحميل ( 0,20,30, | Composite materials are used for many application such as science, synthetic and space and space discovery and many application in all life field, because what it has from a special properties for example, strength, light weight, cost …etc,.This work was

دراسة خواص مستويات الطاقة لنظائر 130 - 140,144 - 150Nd باستخدام انموذج البوزونات المتفاعلة الاول (IBM - 1) == A Study of Energy Level of Nuclear Properties of 130 - 140,144 - 150Nd Even - Even Isotopes By The Interacting Boson Model - 1 (IBM - 1)

Author name: فاطمة ستار جابر
Supervisor name: محمد عبد الامير الشريفي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: In this theses, 130 - 140,144 - 150Nd isotopes have been studies by the interacting boson model (IBM - 1) to determine the energy levels, In addition, by the program IBMT was used for evaluating the reduced electric transition probability B (E2) and the e

تاثير بولي اثيلين عالي الكثافة على بعض الخصائص الفيزيائية لخلائط بولي بروبيلين - بولي مثيل ميثا اكريلايت (PP - PMMA)

Author name: احمد حمزة عباس حمود الغرابي
Supervisor name: مجيد علي حبيب
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: في هذه الدراسة تم تحضير المخاليط البوليمرية (PP - PMMA - HDPE) باستخدام باثقة احادية المحور وبتراكيز وزنية مختلفة wt.% (2,4,6,8) من البولي اثيلين عالي الكثافة. اشتملت الدراسة على اجراء فحوصات التشخيص والاختبار ( FT - IRو.(SEM اظهرت صور المجهر الالكتروني ا | In this study, polymeric blends (PP - PMMA - HDPE) have been prepared by using single screw extruder and at different concentration (2,4,6 and 8) % of high - density polyethylene. The study included a diagnostic test and testing of (SEM and FT - IR). phot

دراسة الخصائص الفيزيائية لبعض مساحيق التجميل وتاثيرها على بشرة الانسان == Study of Physical Properties of Some Cosmetics And Their Effect on Human Skin

Author name: رغد منصور عبد سبتي
Supervisor name: عبد الكريم جواد البيرماني | فؤاد شاكر الجبوري
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تضمن البحث د ا رسة بعض الخصائص الفيزيائية لمساحيق التجميل المحضرة على هيئة اق ا رص مضغوطة بسمك يت ا روح بين mm ( 51.13 2.48 - ( قبل وبعد اضافة بوليمر كاربوكسي مثيل سليلوز : في الخصائص الكهربائية حيث تم حساب التوصيلية الكهربائية المستمرة بقياس المقاومة ال

دراسة الخواص الكهربائية والبصرية للمتراكبات (PVA - PVAC - Ti) النانوية == Study The Electrical And Optical Properties of (PVA - PVAC - Ti) Nanocomposites

Author name: وليد خالد كاظم الشمري
Supervisor name: مجيد علي حبيب
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: In this study, (PVA - PVAC - Ti) nanocomposites have been prepared by using casting method with different weight percentages of titanium nanoparticles. The (PVA - PVAC - Ti) nanocomposites have been diagnosed by different methods such as fourier transform

The Effect of Coupled - Channels In Collisions Between Heavy Lons Near The Coulomb Barrier

Author name: نور هادي عباس
Supervisor name: khalid S. gassim | fouad A. mageed
General topic: Physics
Specific topic: Physics
Degree: Master
Language: English
University location: Babylon
First pages:

التقدير الطيفي لبعض المركبات الفينولية ذوات الاهمية الصيدلانية == Spectrophotometric Determination of Some Phenolic Compounds of Pharmaceutical Importance

Author name: زينب طالب عبد الكاظم السلطاني
Supervisor name: قاسم حسن كاظم
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: يتضمن الفصل الاول مقدمة عن تفاعلات الازوتة والازدواج واهميتها وتطبيقاتها في تقدير المستحضرات الصيدلانية، وتضمن ايضا عرضا لبعض الاستخدامات الطبية والصيدلانية للمركبات الفينولية الدوائية المدروسة واستعراضا موجزا للطرائق التحليلية المستخدمة في تقدير المرك | The first chapter included a common introduction on diazotization coupling reaction and their applications to the determination of pharmaceutical preparations. It also included a display of some of its pharmaceutical and medical uses. The chapter also demonstrates a brief review for the analytical methods that have been used for the determination of thymol, Sodium salicylate and Resorcinol in pharmaceutical preparations.The second chapter comprises to describe a simple and sensitive spectrophotometric method for the determination of thymol in aqueous solution, based on the coupling of thymol with diazotized 4 - bromo aniline in strong alkaline medium to produce an intense yellow colored ,water - soluble and stable Azo - dye which exhibits maximum absorption at 464nm. Beer’s law was obeyed between 0.6 - 7.2 µg.ml - 1, with a molar absorptivity of 3.0284×104 l.mol - 1.cm - 1,average recovery was 100.002%, and RSD was 0.796%, the LOD was 0.04?g.ml - 1 and LOQ was 0.1333?g.ml - 1. The method has been applied successfully for the determination of thymol in pharmaceutical preparations (mouthwashes) and the analytical results were compatible with certified value of pharmaceutical preparations and with a standard method. Moreover, the accuracy and validity of the method was evaluated against a standard method using F and t - tests. It was found that experimental F and t values at 95% confidence level did not exceed the critical values indicating that the present proposed methods have a good accuracy and validity. The third chapter demonstrates the development of simple and sensitive spectrophotometric method for the determination of microgram amounts of Sodium salicylate in some of its pharmaceutical preparations based on the coupling and Azo reaction to form an intense shine - yellow, water - soluble dye based on the coupling of Sodium salicylate with diazotized p - amino benzoic acid reagent in strong alkaline medium The dye formed has a maximum absorption at a wavelength of 452 nm. Beer’s law was obeyed between 2 - 30 µg.ml - 1, with a molar absorptivity of 8.5013×103 l.mol - 1.cm - 1, the LOD was 0.06?g.ml - 1 and LOQ was 0.213?g.ml - 1. The method had a good accuracy and precision, since the recovery was 99.702% and the RSD was 0.854%.. The method has been applied successfully in some pharmaceutical preparations including salicylic acid (topical solution). The analytical results agreed well with British pharmacopia method. Moreover, the accuracy and validity of this method were evaluated against pharmacopoeia method using F and t - tests. It was found that experimental F and t values at 95 % confidence level did not exceed the theoretical values indicating that the present method have good accuracy and validity. The fourth chapter comprises the description of a sensitive spectrophotometric method for the determination of resorcinol by diazotization reaction using a diazotized 4 - methoxy aniline reagent in strong alkaline medium, to form brown water - soluble colored dye. Which exhibits maximum absorption at 448 nm. The linear range was between 0.4 - 4.4 µg.ml - 1, with a molar absorptivity of 2.7844×104 l.mol - 1.cm - 1, average recovery of 101.017%, RSD was 1.02%, LOD was 0.019 µg.ml - 1 and LOQ was 0.065 µg.ml - 1. By the application of the proposed method on the some pure types resorcinol, it was found that the results agreed well with British pharmacopeia method. Moreover, the accuracy and validity of this method were evaluated against pharmacopoeia method using F andt - tests. It was found that experimental F and t values at 95 % confidence level did not exceed the theoretical values indicating that the present method have good accuracy and validity.

تاثير بعض الادوية على بعض الانزيمات الناقلة لدى مرضى السكري النوع الثاني في محافظة بابل == Study Effect of Some Drugs On Some Transfer Enzymes In Diabetes Mellitus Type 2 In Babylon Province

Author name: زينب غالب عبد الكريم الجبوري
Supervisor name: عودة مزعل ياسر الزاملي
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: شملت الدراسة 100 مريضا بداء السكري من النوع الثاني )غير المعتمد على الانسولين( NIDDM، تراوحت اعمارهم بين ) 53 - 35 ( سنة وتم الحصول على النماذج من المرضى المراجعين الى مركز السكري في مدينة مرجان الطبية في محافظة بابل - العراق، بالاضافة الى 35 شخص ا اصحاء | This study involved 100 patients with Diabetes Mellitus type 2, with average of age between (35 - 63) years old. The patients were enrolled in the Diabetes Center in Merjan Medical City, in Babylon Province, Iraq at February 2014 to August 2014. In addition to 50 healthy persons who represent control group, and their age between (35 - 55) years old.The patients were divided into three groups as followingFirst : (28 patients were treated with Metformin (Glucophage) drug).Second : (30 patients were treated with Daonil (Glibeneclamide) drug).Third : (42 patients were treated with Metformin and Daonil).In addition to 50 healthy persons who are selected as control group (without chronic diseases or diabetic and without smoking).The Diabetes Mellitus type 2 was characterized as in WHO criteria which including that all patients in this study, (the patients who are not suffering from hypertension, the asthma, smoking, alcoholism and without using any other drugs over the essential diabetes drugs except that of (Metformin and Daonil) are dismissed in this study). Serum and whole blood were used to estimate the levels of fasting blood glucose, urea, creatinine, proteins, cholesterol, tri glyceride, fatty proteins (HDL - c, LDL - c and VLDL - c) and liver enzymes (ALT, AST and GGT) in the serum, HbA1c was detected in the whole blood. MDA and Glutathione in the erythrocytes. In this study, It was found that there are significant and insignificant differences for each of variables related to Diabetes for different types of drugs. Also, the glutathione S - transferase GST was purified from red blood cells (RBC) of control group by ionic exchange chromatography by means of DEAE Cellulose. After that, the electrophoresis of resulted enzyme was made to assess the molecular weight by using of Sodium Dodecyl Sulfate - Poly Acrylamide Gel2Electrophoresis (SDS - PAGE). The same process was done by using NAITIVIN apparatus to separate the enzyme quantified, and the resulted enzyme from NAITIVIN was treated with the drugs taken by patients. After that, the activity of GST enzyme was measured to show the different drugs effect upon the GST enzyme activity. For the purified enzyme activity after treating it with drugs was 3.62 , 3.57, 3.66 ?mol / min / ml , for each of Metformin, Daonil, and Metformin & Daonil respectively, while the activity of GST purified enzyme without treating with drugs was 3.24 ?mol / min / ml.Through this study, it has been noticed that there is an increasing of enzyme activity after purifying and mixing it with drugs, and this increasing results from the drugs effect upon GST activity, for the enzyme use to transform these poisons (drugs) and resulted compounds outside the body.

تقدير السلينيوم بالطرق الطيفية في نماذج حياتية وبيئية == Estimation of Se By Spectrophotometric Method In Vital And Environmental Samples

Author name: داليا عبد الامير احمد
Supervisor name: صادق جعفر باقر
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: يعد مرض السكري من الامراض المزمنة الواسعة الانتشار في العالم، ومن المشاكل الصحية الرئيسية في معظم دول العالم يضم هذا المرض مجموعة من الاضطرابات الايضية المختلفة (كاربوهيدرات، بروتينات، دهون )، اهمها ارتفاع مزمن في مستوى سكر الدم، حيث ان لتحلل الكلوكوز ف | Diabetes is a chronic disease widespread in the world and lt is amajor health problem. It is agroup of metabolic disorderd including carbohydrates , proteins and lipids. It is considerded as chronic high blood sugar (hyperglycemia). The glycolytic pathway in the cells play an important role to produce the serine which is formed by turning the phosphoglycerate in a series of steps. Serine is behaved as a donor of methyl group catalyzed by serine hydroxyl methyl transferase to produce homocysteine in a series of steps using vitamine B12 and tetrahydro folate (folate cycle) to control the lack of homocysteine that causes high damage to the lining of arteries and other organs , which in turn leads to partial decrease in insulin secretion or lack of insulin receptors. This may effect the eyes , kidneys , blood vessels, and nerves. This research includes people with diabetes type II. admitted to the teaching hospital in Tikrit, Samples have been taken from 40 patients (22 males and 18 females) and (5) samples of healthy males from the staff of the University of Tikrit, The study included two parts : - 1 - Biochemical study : - included the measurement of plasma level of homocysteine from the blood of patients with type II diabetes which showed : - 1 - Significant increase (P

تحضير وتشخيص ليكاندات ازو جديدة غير متجانسة الحلقة مشتقة من 5,4 - ثنائي فنيل اميدازول ومعقداتها مع بعض الايونات الفلزية == Synthesis And Characterization of New Heterocyclic Ligands Based On 4,5 - Diphenyl Imidazole And Their Complexes With Some of Metal Ions

Author name: اسراء نور كاظم وتوت
Supervisor name: حسين عبد محمد صالح
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تضمن العمل تحضير ليكاندين جديدين من ليكاندات الازو العضويه غير المتجانسه المشتقة من البنزامبدازول الاول هو اليكاند 2'] - 2 - (بنزايميدازوليل) ازو [ - 6,4 - ثنائي كلورو فينول Benzimidazolyl) Azo] - 4,6 - dichloro phenol (BIADClP)) 2 - [2' - والليكاند الثا | This study deals with the preparation of two new ligands from the heterogeneous organic azo ligands drived from Benzimidazol.The first) 2 - [6 - Benzimidazolyl) Azo] - 2,4 - dichloro phenol (BIADClP). The second is 2 - [6 - (benzimidazolylazo)] - 2,4 - dibromo phenol(BIADBrP).The heterogeneous chain in both ligands includes two nitrogen atoms. These two ligands have been identification by the proton nuclear magnetic resonance spectra (1H - NNMR) ,the mass spectrum, infrared, visibale ultraviolet as well as the analysis of the micro - elements (C.H.N).And two new series of the solid canine complexes for these ligands. The first series includes six solid complexes of the ligand (BIADClP) with the metallic ions Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II). The second series includes the preparation of six solid complexes of the ligand (BIADBrP) with the metallic ions mentioned above. The effect of the solvent and the complexes stsbility have been studied on the prepared ligands with the passage of time starting from the moment of the metallic ion solution reaction with the ligand solution and up to 170 minutes. Also, the stability constants for these complexes have been calculated spectrally by visbale - ultraviolet spectra.All results show that these complexes have high stability. The infrared spectra of the prepared complexes have been studied and when compared with the free ligand spedtra give obvious changes as these spectra show new bands not already found in the spectra of both ligands and this is due to the occurance of the correlation between the metallic ions under study with the donar atoms [the nitrogen atom azo group near the heterogeneous chain (N3) and nitrogen atom benzimidazole molecule (N3) , the oxygen atom of hydroxyl group in the heterogenous chain ] in the ligand molecule whereas the other band suffered from obvious changes in shape, intensity and location and this is a proof on the occurance of consistency process between the metallic ions under study and the tow ligands. Atomic absorption spectrometer is used for assigning percentages of the metallic ions in the prepared complexes as well as the analysis of micro - elements (C.H.N). These results indicate the great consistency between the percentages theoretically calculated and practically gained. The magnetic sensitivity indicated that Ni(II) - complex and Cu(II) - complex for both ligands have baramagnatic characteristics whereas the Co(III) - complex, Zn(II) - complex, Cd(II) - complex, Hg(II) complex have adia magnetic characteristics. Also , the study deals with the molar conductivity of the canine complexes dissolved in DMF nad ethanol in a concentration (1×10 - 3) molar and in lap temperature. The results show that the complexes of Ni(II) , Cu(II), Zn(II), Cd(II), Hg(II) do not have ionic characteristics and they are solution of non - electrolytes nature whereas the complexes of Co(III) and for both prepared ligands (BIADClP) and (BIADBrP) have ionic characteristics of the electrolytes nature of type (1 : 1) whereas other remaining complexes do not have any ionic characteristics. Throughout the results that have been reached at, certain structural forms for the canine complexes have been suggested.It has been indicated that the azo Benzimidazol ligands under study behave as tridentate ligands where the consistency happens through the N atom benzimidazole chain (N3) and N azo group far from the heterogeneous chain (N3) oxygen hydroxyl group of the homogeneous chain which leads to forming canine hexagonal complexes having octahydral as steric form.The hybridization of these complexes is d2sp3 concerning Co(III) - complexes of the ligands (BIADClP) and (BIADBrP) whereas the other complexes have the hybridization sp3d2 with the tow prepared ligands under syudy. Moreover, the study deals with the biological activity of these ligands and metallic complexes in the growth of four types of bacterial spores (germs) by using the solvent DMSO and deployment technology as anti - bacterial such as Staphylococcus aureuse and Staph.lentus representative for Gram positive bacteria and enterobacte and Escherichia coli representative for Gram negative bacteria. It is found that ligands and there complexes have different effect as antidepressants in the inhibition and the growth of bacteria under study wheras the ligand (BIADClP) gave the ability in inhibition of the growth of bacteria more than the ligand (BIADBrO) this is due to the change of substituted group on the homogeneous chain and their different in both ligands which led to the emergence of such difference in the inhibition and growth of bacteria.

دراسة تخليق ومقارنة نظرية لتفاعلات الاسترة لمشتقات البنزين ثنائي هيدروكسيل ونواتج البلمرة == The Study of Synthesis And Theoretical Comparison Esterification Reactions of Benzene Diol Derivatives And Polymerization Products

Author name: هيفاء عدنان عبد الامير
Supervisor name: سعدون عبد الله عودة | عباس عبد علي الصالحي
General topic: Chemistry
Specific topic: Organic Chemistry
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تضمنت الرسالة تحضير ليكاندات ازو جديدة] 2 - هيدروكسي - 3 - ((5 - ميركبتو - 4,3,1 - ثايادايازول - 2 - يل) دايازينيل) - 1 - نفثالديهايد[ (HL1) المشتق من 5 - امينو - 1،3،4 - ثايادايازول - 2 - ثايول مع 2 - هيدروكسي - 1 - نفثالديهايد. و3] - ((1،5 - ثنائي مثي | In this work new azo ligands [2 - Hydroxy - 3 - ((5 - mercapto - 1,3,4 - thiadiazol - 2 - yl)diazenyl) - 1 - naphth aldehyde] (HL1) derived from 5 - amino - 1,3,4 - thiadiazole - 2 - thiol and [2 - hydroxy - 1 - naphthaldehyde. And 3 - ((1,5 - Dimethyl - 3 - oxo - 2 - phenyl - 2,3 - dihydro - 1H - pyrazol - 4 - yl) diazen yl) - 2 - hydroxy - 1 - naphthaldehyde] (HL2) derived from 4 - amino - 1,5 - dimethyl - 2 - phenyl - 1H - pyrazol - 3(2H) - one and 2 - hydroxy - 1 - naphthal dehyde have been prepared. And it’s metal complexes of with VO2+, Cr3+, Mn2+, Co2+, Ni2+ and Cu2+ have been prepared with ligands. The ligands were characterized by physic - chemical spectroscopic techniques [(C.H.N.S.), FT - IR, UV - Vis, 1H and 13C - NMR, Mass and TGA DTA curve]. And characterized complexes by Elements micro analysis (C.H.N.S.), FT - IR, UV - Vis, Mass and TGA DTA curve, Flame atomic absorption, Magnetic susceptibility, Chloride containing and Molar electric conductivity.The spectral data suggested that the ligand (HL1) as a neutral bidentate is coordinated with metal ions through the nitrogen atom of azo group and oxygen atom of phenolic group for all complexes, the proposed geometry that the Mn2+, Co2+ and Ni2+ complexes have tetrahedral geometries, and the Cr3+ complex have octahedral geometries and the VO2+ complex have square pyramidal geometries, and Cu2+ complex have square planer geometries, molar ratio ligand : metal (1 : 1).The (HL2) as a neutral tridentate ligand is coordinated with metal ions through the nitrogen atom of azo group, oxygen atoms of phenolic group and carbonyl group of and for all complexes only with VO2+ complex is bidentate, the proposed geometry that the Mn2+, Co2+, Cu2+ Ni2+ and Cr3+ complexes have octahedral geometries, and the VO2+ complex have square pyramidal geometries, molar ratio ligand : metal (2 : 1). The effect of the time on the stability for the solution of complexes has been studied and the steady of the stability for all the metal complexes through the time has been noticed.Hyper Chem - 8 program has been used to predict the structural geometries of the compounds in the gas phase. The total energy, dipole moment, electrostatic energy, heat of formation, and binding energy at 298?K, was used to evaluate the vibrational spectra of the free ligands and compare the theoretically calculated wave numbers with the experimental values. From previously information we can suggest the stable geometries for all compounds and measured their bond length between their atoms in molecules.

تقويم فعالية الخميرة Candida albicans من المسح المهبلية للنساء في حيوية النطف واحداث العقم

Author name: رقية يحيى عبد الشهيد الطفيمي
Supervisor name: زيدان خليف عمران المعموري | فارس ناجي عبود الهادي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: The present study aimed to identify some of the molecular dynamic qualities and the type of Candida albicans and determine its virulence factors using some of the phenotypic and molecular techniques and their impact on the semen and causing infertility.The collection of 173 swab of the vagina and the cervix was where 97 women from women with primary infertility, including 78 women with positive and 19 women with a scanning negative.kmaamlh control and 76 women with secondary infertility, including 49 with positive screening women and 27 women with a scanning negative.kmaamlh control, women who Rajan unit infertility in Babylon, a maternity hospital and children and private clinics in the province of Babylon.The use of agricultural methods to isolate and diagnose 127 isolation dating back to Candida vaginal as well as the use of CHROMO agar medium test and test tobacco Agar to distinguish between certain types of Candida, was detected on some virulence factors such as the composition of the Germ tube and spores Alclamadah and production of enzymes case of fat (Alphusfullaypez) and the formation of membrane It was vital to draw your DNA candidiasis and the use of PCR technique where the starters used by the general whiteners, especially for the purpose of diagnosis isolates of Candida albicans and genetic profiling of yeast Candida depending on the variation in the ITS region area. Showed isolate and diagnose types of eggs in women with infertility initial results of 48 women infected with C.albicans by (61.53%), while 30 women is infected Non - C.albicans by (38.46%) either in women with secondary infertility showed the 29 women infected with C.albicans by ((59.18% and 20 women is infected non - C.albican by (40.81%). the results showed the center CHROMO agar medium isolates of Candida species C.albicans was once a green color and isolates C.tropicalis with blue color and with C.krusei color pink and showed C.glabrata violet and C.parapsilosis that showed white.And C.albicans isolates showed C.albicans result is positive for the production of germ tube and spores Alclamadah as well as the center of tobacco Acar which they appear yeast C.albicans white colonies free of filamentous structures, a test of this kind without other types of Candidapositive test.. While the production of enzymes showed a case of fat test (Alphusfullaypez) as a result of the positive isolates C.albicans and C.krusei The results showed that the incidence of candidiasis in women with infertility first was more ratio than the unemployed in secondary infertility. It was the incidence of type C.albicans is the largest in all of the women with primary infertility and secondary infertility women initial rate of 61.53% and 59.18%, respectively, followed by injury type C.glabrata by 17.9% in women with infertility first and 18.36% in secondary infertility. Distributed cases of four age groups and was the highest rates (47.91% (in women with infertility and the first (55.17%) in the women's secondary infertility is located within the age group (30 - 21) years old. In the case of a period of infertility and distributed cases into three periods and was the highest rate of infection is the period (5.1), where the percentage (81.81%) in infertile women was less than the rate of infection is the period (15 - 11), where the ratio of (6.49%) in infertile women. The results showed that the rate of infection C.albicans were higher in women with and who not to say on treatment by (65.33%) of either women with and who took up treatment (53.84%). As well as the incidence is higher in women infertile is Mentzmat appeared menstrual cycle by (63.75%) The women infertile women with regular menstrual cycle and to have a lower rate (55.31%). The incidence of rural women by (67.56%) higher than the women of the city that are represented (58.49%).It was three concentrations preparation 3×107, 5× 107, 7×108cfu/ml of all isolated from C.albicans and raw leach ate Khmer and observe their effect on semen and then finding the value of MED50 effective dose)) concentrations C.albicans way to draw a dose - of responsiveness was found that the concentration curve (107× 5 cfu/ml) top influential focus on movement and Sperm Viability. It was the influence of the C.albicans test on semen Concentration 107× 5cfu/ml over time (after 30 - 60 minutes) and a leaky yeast and after the screening device Alkazh Computer aided sperm analysis (CASA) where he found the impact of the C.albicans on sperm movement, Rapid progressive (a), Slow progressive (b) and Sperm Viability (V) over time after the text of the clock and watch.The pair initiator year ITS4 - ITS5 in the diagnosis of all isolates representing the total isolates succeeded as a return to form of the genus Candida spp. Where the results of the polymerization 520 bp. The pair initiator Specialist in the diagnosis of Candida yeasts belonging to the type C.albicans and succeeded when he polymerization products 344bp, while the rest of the species is C.albicans did not show any polymerization products.Showed genetic profiling products for the ITS using a pair of initiator ITS3 - ITS4 where showed variation in the ITS region area for the pair in the public initiator profiling C.albicans under study

فحص عوامل عنق الرحم باستخدام فحصي اختراق النطف واتصال النطف بمخاط عنق الرحم للازواج العقيمين == Examination The Cervical Factors By Using Sperm Penetration Test And Sperm Cervical Mucus Contact Test In Infertile Couples

Author name: الاء عمران مطلك الحسناوي
Supervisor name: فارس ناجي عبود الهادي | بشرى جابر الربيعي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: The present study was conducted in Infertility unit / Babylon Hospital of Gynecology and Children in a period 21/1/2013 to 15/1/2014. The study involved communiqués tests for males and females complaining from unexplained infertility , in which seminal fluid analysis were normospermia. The study include 45 seminal fluid specimens used in connecting test (SCMCT) and penetrating test (SCMPT) with the specimens of cervical mucus of their wives. Cervical mucus aspirating in day 12 - 14 of menstrual cycle. The study aimed to evaluate the husband's sperm activity and concentration in vitro with cervical mucus. Seminal fluid analysis were achieved and then semen specimens used to performed two in vitro tests : SCMCT and SCMPT. sperm parameters ( sperm concentration and sperm motility grade a, b, c, and d) were evaluated in each mentioned test and compared the results with the values of sperm parameters in seminal fluid analysis (control). The statistic results showed 60% of the study specimens of SCMCT was negative and 40% positive, while in percentage of positive results was 33% and 67% negative in penetrating test. The results revealed a significant (p<0.001) decrease of sperm concentration and grade a and b sperm motility, a significant (p < 0.001) increase of sperm motility grade c and immotile sperm grade d in connecting test with cervical mucus compared to control. The results of penetrating test showed significant (P< 0.001) decrease of sperm concentration in the brim, medium and distal region of cervical decrease (p<0.001) of sperm concentration in both medium and distal region compared to brim region, and a significant (p < 0. 001) decrease in distal region compared to medium region ; A significant (P < 0.001) decrease of grade a + b motility percent in all three regions of cervical mucus area compared to control, while no significant differences (P > 0. 05) between brim, medium and distal region of cervical area ; Only grade C sperm motility in distal region showed a significant decrease (P < 0. 001) compared to control and both brim and medium regions. The results revealed significant increase of grade d (P<0.001) in brim and medium region (P < 0.01) compared to control, while there was a significant decrease (P< 0. 001) in distal region compared to control, brim and medium regions. The correlation (r) study of sperm concentration and sperm motility ( grade : a + b, c and d) with age and infertility period in SCMCT showed non significant (P > 0. 05) correlation, except the correlation between a + b grade sperm motility and age it was noticed a significant negative correlation (P < 0. 05) and positive correlation(p> 0.05) between grade c sperm motility and age. The study of linear correlation of sperm parameters in penetrating test showed a significant(P<0.05) negative correlation between grade a+b sperm motility and age in brim and medium region of cervical mucus area. Except this, there are no significant correlation (P>0.05) between sperm concentration and sperm motility for all grades (a + b, c and d) with age and infertility period in both brim and medium regions It was concluded that both test : SCMCT and SCMPT were necessary to diagnosis the causes of infertility when there were normal finding of both seminal fluid analysis and female criteria. mucus area compared to sperm concentration in control, also a significant

تاثير مستخلصات المركبات التربينية والقلوانية والفينولية الخام لنبات الزريج Chrozophora tinctoria L. في بعض جوانب الاداء الحياتي لحشرة الذبابة المنزلية Musca domestica L.(Diptera : Muscidae) مع عزل وتشخيص المركبات الفعالة باستعمال تقنية الكروموتوغرافيا الس == The Effect of Crude Extract For, Terpenoids Alkaloids And Phenolic Compounds of Chrozophora Tinctoria L. On Some Biological Aspects of House Fly Musca Domestica L. (Diptera : Muscidae) And Isolation And Identification of Active Compounds By Using High Per

Author name: اسيل كريم جبار السلطاني
Supervisor name: هادي مزعل خضير الربيعي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: اجريت سلسلة من التجارب المختبرية في مختبر بيئة الحشرات/كلية العلوم للبنات/جامعة بابل خلال عام 2014 - 2015 تضمنت اختبار فعالية مستخلصات المركبات التربينية والقلوانية والفينولية الخام لنبات الزريج Chrozophora tinctoria في بعض معايير الاداء الحياتي لحشرة الذ | A series of laboratory experiment were conducted in the laboratory of insect /college of Science for women /University of Babylon during the year 2014 - 2015 The present study conducted to evaluate the activity of crude extract of Terpenoid, Alkaliod and Phenolic compounds of Chrozophora tinctoria on some biological aspect of Musca domestica and histological study for effect crude phenolic compound on ovaries as well as isolation and identifaction of active compounds in these extracts.The extract of crude terpenoid compound was more effective than crude alkaloid and phenolic compound in different critirra of biological perphormance. The mortality rates of eggs in the extract of terpenoid alkaloid and phenolic compound at concentration 1mg/ml were (90, 63.7, 39.6)? respectively compared with (0.4, 0.8, 0.8)% in the control tretmentsThe mortality rate of first larval instar was (57.64, 57.00, 26.85)% respectively when treated with crude terpenoid, alkaloid and phenolic compounds at concentration 1mg/ml compared with (0.8, 0.8, 0.8)% in the control treatment.The mortality rate of pupae was 18.06% when treated with crude terpenoid extract at concentration 1mg/ml compared with 0.4% in the control treatment. The cumulative mortality rates of immature stage when treated with crude terpenoid, alkaloid and phenolic compound were (100, 100, 42) % at concentration 1mg/ml compared with (15, 14, 12)% at the control treatment.Developmental period of immature stage was 36.6 days when treated with crude alkaloid extract at 0.25 mg/ml compared with 10.2 days in the control treatment.The pupal weight and female productivity were reduced from 0.1gm, 122.6 egg/female in control treatment to 0.556 gm, 0 egg/female respectively when treated with crude alkaloid extract at 0.25 mg/mlThe different concentration of crude terpenoid, alkaloid and phenolic compound were effective in the performance of eggs deposition, the nummber of eggs decreases in crude extract terpenoid, alkaloid and phenolic compounds at the concentration in same space was (0) egg/femal for all extracts at concentrion 1mg/ml comparted with (117, 118, 106) egg/femal in the control treatment, while was (0) egg/femel when treated in different concentration of terpenoid, alkaloid alone of consentration 1mg/ml compared with (120, 122) egg/femel in the control treatment.The number of eggs was (37) egg/femel when treated with phenolic compounds at same concentration compared with (120) egg/femel in the control treatment.The study included the effect extracts activity for crude phenolic at concentration 1mg/ml in histological structure for insect overies, The length and diameter of ovary and ovares follicles at (24, 96) houres after emergency while the length and diameter of ovary was (625, 468.75)µm respectively at 24 houre at concentration 1mg/ml compared with (652.173, 565.217)µm in the overy of femel in the control treatment. The length and diameter of ovaries follicles were (116.66, 72.22)µm respectively in the femel overy for control treatment, while was not distingwish for follicles female which were produced from treatment. while in (96) hour after emergency the length and dimeter of ovary (1114.285, 971.428)µm respectively when treated with extract compard with (1688.88, 1481.48)µm for overy of female in control treatment.Isolation and identification of active compounds in the crude extract of terpenoid, alkaloid and phenolic compounds, of Chrozophora tinctoria by using high performance liquid chromatography (H.P.L.C), Five compounds isolated from crude terpenoid extract was Teter methyl hydrazine compound which had retention time(1.333) mintue which was the first compound, while seven compounds isolated from phenolic compounds The gallic acid compound had retention time 1.34 mintue, which was the first compound isolation.Two compounds were isolated from crude alkaloid compounds which were berberine and isoquinoline, berberine compound had retention time 2.51 mintue which was more concentration than other compound.

دراسة تصنيفية لبعض اجناس العائلة المركبة Asteraceae في العراق == Taxonomical Study of Some Genera From Asteraceae In Iraq

Author name: اشواق فالح خزعل العجيلي
Supervisor name: عبد الكريم خضير البيرماني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تناول هذا البحث دراسة تصنيفية مقارنة لستة اجناس تعود للعائلة المركبة Asteraceae وهي Chrysanthemum L. وGazinia وGerbera وLeontodon L. وTagetes L. وZinnia L. وتميز كل جنس من تلك الاجناس المذكورة باحتوائه على نوع واحد فقط في العراق وهيL. Chrysanthemum coro | The present investigation comprises systematic study for six genera belong to Asteraceae as follow : Chrysanthemum L., Gazinia, Gerbera, Leontodon L., Tagetes L. and Zinnia L. Each genus of those genera described as containing only one species in Iraq, Chrysanthemum coronarium L., Gazinia splendens Gaertn, Gerbera jamisoni F.Gmel, Leontodon laciniatus L., Tagetes patula L. and Zinnia elegans L. respectively. The study was conducted in the laboratory research of plant taxonomy / College of Science women / Babylon university for the period January 2015 until August 2015.The study included morphological, anatomical, palynological and chemical characteristics as well as numerical taxonomy have been done for all species. The importance of taxonomic to studying variations in roots, stems, leaves, inflorescences, involucre bracts, flowering and fruiting parts and Indumentum were discussed. Results showed that many of the vegetative and reproductive characters have the importance taxonomic value for isolating genus from each other such as presence and absence of the stem and the nature of the basal and cauline leaves in addition the present of leafy stipule as well as the nature of Indumentum.Anatomical characters of leaves epidermises and the cross section of the stem, leaf blade, petiole and peduncles, in addition leaves venation have been studied and their taxonomic importance were assessed.The study also found the pollen grain was tricolporate in general, but the presence of spine or not had an important taxonomic to isolate Gerbera jamisoni by smooth surface compared to the rest of the species, while possible to isolate Gazinia splendens and Leontodon laciniatus by the presence of the bridges in pollen surface. On the other hand it has been the study of chemical content in each of the leaves and flowering heads of the species studied using Gas Chromatography technique (GC) to detect the terpenoid compounds, The presence of compound Nerolidol, Terpinolene in the species.. Gerbera jamisoni, Zinnia elegans emphasized the importance taxonomic chemical content at the level of species and genera.The species were numerically treated on the basis of number of selected characteristic from various studies to obtain the similarity and differences ranges between different species. Dendrogram was drawn for this purpose. As a result of preceding observation this study confirmed the diagnosis of six genera within Asteraceae family in Iraq. Finally all the taxa were treated taxonomically, and the key was prepared for all the taxa studied.

تقييم كفاءة بعض المستخلصات النباتية وعقار الميترونيدازول Metronidazole في حيوية الاميبا الحالة للنسيج Entamoeba histolytica خارج الجسم الحي In vitro مع دراسة جزيئية لتحديد بعض الانواع التابعة لها في محافظة بابل == Evaluation of Some Plant Extracts And Metronidazole Drug In Vitro of Entamoeba Histolytica Viability And Molecular Study For Some Species Determination In Babylon Province

Author name: سحر عباس حسين المعموري
Supervisor name: هادي مزعل خضير الربيعي | احمد خضير عبيس الحميري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: اجريت الدراسة الحالية للمدة من تشرين الاول 2013 ولغاية تموز 2014 في كلية العلوم للبنات / جامعة بابل, اذ تم الحصول على طفيلي الاميبا Entamoeba من عينات المرضى المصابين والمراجعين لمستشفيات بابل للاطفال والولادة ومستشفى ابن سيف للاطفال والمركز الصحي الحسين | The present study was conducted during the period from October 2013 till July 2014 in the College of Science for women, Babylon University, the Entamoeba stool samples were collected from patients that attending to Babylon Maternity and pediatric hospital, Ebn - Saiff hospital and Abi - Gharaq and Al - Mussaib of primary health center, Babylon province. The parasites were detected by direct smear method, 130 stool samples were collected that contain blood and mucus. The overall percentage incidence of E. histolytica (80.8%). No significance differences of infection rates in males and females and the highest rate of infection was in (1 - 10) year age group for males and females (82.9% and 39.1% respectively) while the lowest infection rate was in (51 year and more) for males and females (33.3 %, 6.7 % and respectively).Three plants extracts (cooled and Boiled) water (Euphorbia helioscobia, Eucalyptus gloubules and Mytrus comminus) evaluated at the culture media (Beef liver infusion media) that grown with E. histolytica. The results revealed the E. helioscobia boiled water extracts the efficient extracts to killed the all parasites in the second day from experiment beginning with 10 mg/ ml concentration and the next efficient plant extracts (boiled water) its E. globules and M. commines was the third day with 5 mg / ml concentration. The Metronidazole showed to killed all parasites in the third day with 1 microliter / ml. and from this present study showed that the successful growth of E. histolytica in culture media for some day and finished (Killed) at the fiveth day from experimental beginning.Isolation and identification of some terpenoid compounds were detecting in the present study belong E. helioscobia plant extracts (Camphene, ???pinene, Euphornin, ?? caryophyllene, Myrecene and ??humulene) by (HPLC).Molecular study was conducted to determined three species of Entamoeba by using Polymerase chain reaction for ten stool samples that primarily detected with Amoebic dysentery by direct smear method and the following results for molecular study, the highest overall percentages its E. dispar with (7/10) mean 70% whereas the E. histolytica and E. moshkoviskii its (6/10) mean 60 % for each one, as well as existence the double infection or tertian infection with these species for Entamoeba.

تقييم كفاءة بعض المواد الكيميائية ومستخلص الماء المغلي والبارد لنبات الصبار (L.) Opuntia ficus indicaعلى حيوية الرؤيسات الاولية للمشوكة الحبيبية Echinococcus granulosus خارج الجسم الحي In == Evaluation The Efficiency of Some Chemicals And Boiled And Cooled Water Extracts of Cactus Opuntia Ficus Indica (L.) On The Protoscolices Viability of Echinococcus Granulosus In Vitro

Author name: اشجان محمد عريبي الخالدي
Supervisor name: احمد خضير عبيس الحميري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: Evaluation study was conducted for some chemical solutions (Formalin, Sodium chloride NaCl and Silver nitrateAg NO3) as well as cooled and boiled cactus plant axtracts for the period from March 2014 till October 2014 on the In vitro protoscolices viability for Echinococcus granulosus by using preservative media solution (Kreb ringer+hydatid cyst fluid, 4 : 1, respectively). Protoscolices were collected from the infected sheeps livers in Al - Hilla and Al - Najaf abattoir, and protoscolices suspention was added to each concentration of the therapeutic agents alone which were used for different time periods. The percentage of the viability of protoscolices was measured by using the eosin aqueous stain (0.1%). The results revealed that the viability of protoscolices percentage (90.72%) and after five days the viability of protoscolices was 60.9 - 68 % and from results showed that the silver nitrate solution with concentrations (100, 125, 250, 500, 1000) ?g/ml was the highest solution which succed in killing the protoscolices 100% at the first hour of experiment, followed the sodium chloride solution with concentrations (100, 125) mg/ml at the first hour, then the boiled cactus plant extracts with concentration 10 mg/ml showed high efficiency and succeded to killing the protoscolices 100 % after 48 hours from another cooled cactus plant extracts with concentration 10 mg/ml which succeded to killing protoscolices after 72 hours as well as the albendazole drug with concenrtation 400 mg/ml which succeded in killing the protoscolices 100 % after 72 hours while the formalin solution with concentration 40 % succeded in killing the protoscolices 100 % after 96 hours from the experiment

التمنيع والتحدي لبكتريا Citrobacter freundii فاقدة الجدار المسببة لخمج السبيل البولي == Immunization And Challenge For Walled Citrobacter Freundii And Walled Defective That Causing Urinary Tract Infection

Author name: عبد الرسول عويد عداي الشبيب
Supervisor name: قاسم نجم ثويني
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: Cryptic bacterial infection has been studied in urinary system to Human, where 50 underwent urine samples from patient mostly based consulting auditors Hilla public Education Hospital for the period from November 2012 until Feb. 2013. Patients ranged in age from 20 - 50 years males and females bacterial infection rate was 70% distributed between the cell wall defective bacteria had 38% and 32% cell - wall bacteria and the results showed 30% as negative cases. Amicroscopic examination of the urine sludge Showed clear sovereignty of non - specialized immune response type of mixed cell 34% followed by mononuclear cell 30%. Samples were cultured on routine media (Blood agar , Macchonky agar) and Modified media (Variant agar, Variant broth). Out put diagnosed by bacterial system diagnostics, phen - otypic, microscopically and biochemistry by using the special test for microorganism diagnosis and using API 20 E and the use VITEK 2 to confirm some samples results. Characterized by colonies of cell wall defective bacteria grow on variant agar it resembles fried egg which could seen after staining Deinse stain. Citrobacter freundii recorded 12%, the highest rate among cellwall defective isolates. Sensitivity test for bacterial isolates were screened from U.T.I both two type (CWB & CWDB) showed the highest rate of sensitive to Impinem 100% represented CWDB, as well as the highest rate of resistance to Ampicillin 100% represented by the same group of isolates. experimental study with urinary tract infection by injecting bacterial stuck of Citrobacter freundii to the three groups of rabbits : first group ( 3 Rabbits ) : immunized with viable surface antigen extracted from CWDB, second group : ( 3 Rabbits ) immunized with heat killed surface antigen extracted from CWB, the third group (4 Rabbits) represented control group it been treated with sterile normal saline. the injection process through urethra, and then underwent a histological study, was histological damage to the members of the animal - derived non - immunized (control group) seemed clear was the appearance of symptoms of the total cellular infiltration and disruption of the mucous layer and epithelial and other symptoms. The immunized animals have shown their ability to defy injury. It is the study of serological reactions to bacterial antigens Citrobacter freundii immunized with vaccines for rabbits given high caliber agglutination with its specialized vaccines and low with adversity and that the absorption and the corresponding and variant antigen does not remove the agglutination effectiveness with immune sera prepared suggesting that the absorption is incomplete and that the difference between the wall and a wall defective in terms of antigenic is quantitative and qualitative.

التشخيص الجزيئي للخمائر والاعفان المرافقة لمخلفات الطيور وتقييم كفاءة بعض المستخلصات النباتية ضد خميرة Cryptococcus == Molecular Identification of Molds And Yeast Associated To Birds Wast And Evalution Efficiency of Some Plant Extract Against Cryptococcus

Author name: رسل عصام علي الظاهر
Supervisor name: زيدان خليف عمران المعموري
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص الاعفان والخمائر المرافقة لمخلفات الطيور باستخدام بعض التقنيات المظهرية والجزيئية. كما تم اختبار فعالية المستخلصات المائية لنبات وعرك لوية والقرنفل والبابونج في تثبيط نمو خميرة Cryptococcus neoformans.تم جمع 190 عينة من م | The present study aimed to isolation and diagnosis the molds and yeast from birds extract by use some of phenotype and molecular techinces. As well as the aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree.to inhibition Cryptococcus neoformans growth there is about 191 isolate was been collected from Babil and Karbala included 30 isolate (10 isolate hand printing and 10 isolate from ambiend air of bird brought zone, and 10 isolate from cough of men they are dealing with birds in pitry dish containing SDA media. The other 161 isolate was been collected from the wet and dry bird extract from the different environment.Culture method was used in the diagnosis as CHROMagar media to diagnosis Candida spp. Also used the haemolysis test of Macrorhabdus ornithogaster yeast and phospholipase and Urease test of Cryptococcus neoformans yeast.And used the molecular methods as DNA extraction and used PCR technice by use universal and specific primers.Results of analysis isolation and diagnosis showed that the maximum of present appearance was for Candida spp. 45.34% and molds Rhizopus sp. appearance percent 27.32% and frequency percent 28.21% and 3.65% consequently M. ornithogaster appearance percent 4.96% and frequency percent 35.77% while the other molds and yeast have appearance percent between 0.62 - 19.25% and frequency percent between 0.07 - 19.75% and the result of CHROMagar for Candida gives different color C.albicans was green color and C.krusei was pinki color.Cryptococcus showed apositive result in phospholipase and Urease test. haemolysis test of Macrorhabdus yeast was positive the hot aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree have the activity to inhibit the growth of Cryptococcus neoformans, but the more activity was east indian scrow tree with concentration 4%.The pair primer CAP60 succesful to diagnose Cryptococcus neoformans It was 603bp and primer AGY1\Sm2 successful to diagnose Macrorhabdus yeast

الاضطرابات الفسلجية والاجهاد التاكسدي لدى النساء في سن بعد الياس في محافظة بابل == Physiological Disturbances And Oxidative Stress In Postmenopausal Women In Babylon Province

Author name: سارة حاتم عجيل خضير
Supervisor name: داخل غاني عمران الوطيفي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: يتميز سن الياس (Menopause) لدى الاناث بتوقف الحيض وتصبح الانثى غير خصبة بسبب فقدان جميع الحويصلات المبيضية (Ovarian follicles) والانخفاض الشديد لهرموني الاستروجين والبروجسترون صممت الدراسة الحالية لفحص الاضطرابات الفسلجية المختلفة والتي تشمل بعض المعايير | Menopause marks the time in women's life when her menstruation stop and she is no longer fertile because of exhaustion of ovarian follicles and sharply decrease in ovarian production of estrogen and progesterone.The present study was designated to determinate different physiological disturbances which occurring in some hematological and biochemical parameters of postmenopausal women. A total number used was 130 women, 100 women were postmenopause and subdivided into four age groups (46 - 50 year, 51 - 55 year, 56 - 60 year, and 61 - 65 year). Thirteen women were premenopause , used as a control group, their ages ranged between 20 - 30 years. In all age groups of postmenopausal women, it have been found that the levels of estradiol hormone were progressioly decreased (p<0.05) when compared with control group. Regarding hematological parameters, RBCs count was insignificantly increase (p>0.05) in all age groups of postmenopausal women. Levels of hemoglobin concentrations and packed cell volume were significantly increased (p<0.05) in all age groups of postmenopausal women compare to control groups. Blood platelets count recorded insignificant fall (p>0.05) in most age groups of postmenopausal women.As for, red blood indices (MCV, MCH, and MCHC) explained insignificant differences (p>0.05) of most menopausal women in a comparison with those control group. Total white blood cells have insignificant elevation (p>0.05) of most groups except 56 - 60 years groups. Regard to differential white blood cells, Levels of lymphocytes and monocyte have been showed insignificant drop (p>0.05) of the level of lymphocytes and significant increase (p<0.05) of first and second age groups in the levels of monocytes of postmenopausal women. Granulocytes white blood cells have been indicated different changes : Nentrophils were significantly higher (p<0.05)in all age groups of postmenopausal women, where as, eosinophils and basophils were insignificantly different (p>0.05) in most age groups. Concerning biochemical changes, Total serum proteins, Albumin, and globulin have been showed significant lowering (p<0.05) in all age groups of postmenopausal women. Statistical analyses showed a significant rise (p<0.05) of serum uric acid and urea levels in most group of postmenopausal women. Measurments of enzyme activities involved in liver (ALT and AST) were significantly hightened (p<0.05) in most age groups of postmenopausal women, Whil, ALP activities recorded insignificant increase (p>0.05) in all age group of postmenopausal women. Determinations of minerals showing a significant increase (p<0.05) in the levels of calcium and insignificant increase (p>0.05) in the levels of phosphorus in all groups of post menopausal women. Analyses of electrolytes have been explained a significant elevation (p<0.05) of the sodium and non significant difference (p>0.05) of the potassium in all groups of postmenopausal women.The present study involved determination specific and essential trace elements, Levels of copper explained a significant decrease (p<0.05) in all age groups associated with a significant increase (p<0.05) of zinc levels in postmenopausal women. Inversly, Values of magnesium were insignificantly different (p>0.05) in all age groups of postmenopausal women. The present study selects specific parameters to explaine oxidative stress in postmenopausal women. Levels of malondeheyds were significantly elevated (p<0.05) in all groups of postmenopausal women, and correlated negatively (r= - 0.102) with level of estrogen. Values of reduced glutathione showed a significant decrease (0.05) in postmenopausal women and correlated positively (r=0.64) with the estrogen hormone. Analyses of antioxidants enzymes have been showed a significant decrease (p<0.05) in the activity of catalase enzymes of all groups of postmenopause. Also, the activity of glutathione peroxidase was significantly decrease (p<0.05) in postmenopausal women and its correlation coefficient was r=0.263. In conclusion, changes summarized above may be attributed to deficiency of estradiol hormone in postmenopausal women which resulting in different physiological disturbances and these changes become aggravated and exacerbated because of oxidative stress occurring in decrease of estradiol hormones.

دراسـة تصنيفية للجـنـس Rhagadiolus Scop. (Compositae) في العراق == A Systematic Study of The Genus Rhagadiolus Scop. (Compositae) In Iraq

Author name: اوراس عبد السيد مهدي الحسيني
Supervisor name: عبد الكريم خضير البيرماني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تناولت الدراسة الحالية دراسة اربعة انواع للجنس Rhagadiolus Scop. في العراق دراسة تصنيفية مقارنة شملت الصفات المظهرية والتشريحية وحبوب اللقاح والبيئة والتوزيع الجغرافي للانواع اضافة الى التصنيف العددي.شملت الدراسة المظهرية صفات الجذور والسيقان والاوراق وا | The genus Rhagadiolus Scop. in Iraq has been systematically studied. Work involving comparative morphology, anatomy, palynology, ecology & geographical distribution and numerical taxonomy has been presented. The morphological study includes studying the characters of root, stems, leaves, inflorescences, phyllaries, flowers, and fruits in addition to indumentums. It has been clear that the characters of stems, leaves, involucres bracts and fruits have a taxonomic importance.Also it has studied some of the micro morphological characters and their taxonomic importances were assessed.The present study showed that the pollen grains of all species were found to be tricolporate with echinate surface & of little taxonomic value. This study also includes characters of the epidermal leaf and stems, indumentums, venation and transverse section of stems and leaves discussion were also included, the taxonomic importance of each character & its variation, it is shown that these characters have an importance assessing morphological characters to isolate these species.Data obtained from herbarium specimens, literature and trips was utilized to elucidate the ecology of the species. Maps of geographical distribution were prepared for all species studied. It has been shown that R.angulosus was the most widely distributed species in the country. The species under current study were numerically treated ; dendrograms and polygonal graphs were drawn for the species of the genus. In conclusion (4) species of the genus Rhagadiolus : R.angulosus, R.edulis, R.hamosus and R.stellatus were recognized for Iraq, taxonomic treatment has been carried out for all Rhagadiolus species and key was designed for them too.

تاثير نقص البروتين في بعض التغيرات النسجية والسريرية والوظيفية في ذكور الفئران البيض == The Effect of Protein Deficiency In Some Clinical, Histological And Physiological Changes In Male White Mice

Author name: نسم عماد دايم الفؤادي
Supervisor name: فاضل فرهود مكي الجبوري | هاشم محمد عبد الكريم العلاق
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: The present study is designed to know the effect of protein deficiency on mice males through study the clinical histological changes of some organs including (liver, kidney, stomach and testes).In addition of study the blood and biochemical parameters, where they were sheltering animals in the animal house of the branch of physiology / College of Veterinary Medicine / University of AL - Qadisiya and the number was 90, all of the mice are males and age (28 - 30) days kept for two weeks before starting the experiment for the purpose of acclimatization was given during this period, diet control, then the experiment began between 01/05/2014 until 19/2 / 2014, and at weights ranging from start racing experience between 17 - 18 g, all in a period of growth.These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different period as follow (15, 30, 45)day.Experiment takes study of changes in body weight of animals and some selected organs also study histological changes for studied organs, also physiological blood parameters such as PCV, Hb, GCS and TSP.Results showed significant decrease (p<0.05) in weight body rate of animals, also in the rate of selected organs means compared with control group. The second treatment for 45 days most significant in decreased of body weight and organs weight if it compared with first treatment in all periodsHistological procedures showed that clinical and histological changes are graduated by the severe of protein deficiency and period of time. The histology investigation for liver showed that congestion of blood vessels accompanied with dilation of central vein and portal vein, and necrosis of the hepatic cells and destroyed the general total structure of liver. The section of kidney showed that necrosis and destroyed in renal tubules and epithelial cells in the lumen of tubules and bleeding in renal tubules and clear shrinkage in glomerulus. There are histological changes in stomach by necrosis and desquamation for gastric cells in both period (30, 45) days, while there are no histological clinical changes in both doses in period (15 days) for exposure. Histological sections of testes showed induction inhibition of spermatogenesis and desquamation of germ cells and congestion in the interstitial tissue and dilation of lumen of seminiferous tubules in both periods (30, 45) days, while there is no any changes in both first and second treatment in period of (15)days.In contrast, the hematological parameters showed that the concentration of Hb, PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.Biochemical tests obtain significant decrease (p<0.05) in blood glucose level in both treatment first and second in first period. Also the results showed that no significant increase in first treatment in both periods (30, 45) days compared with control group, while there is significant increase in the level of blood glucose in the second treatment in both periods (30, 45) days compared with the control group. Also did not score a significant decrease (P <0.05) in the total serum protein in thefirst treatment in the first period (15) days, compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while found a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group.It can be conclusion that the exposure for protein deficiency due to clear clinical histological changes for studied organs and some changes in some hematological and biochemical parameters in white mice which treated with protein deficiency which it causes these symptoms

انتاج انزيم الفوسفاتيز القاعدي من العزلة المحلية لبكتريا Bacillus sp. وتنقيته جزئيا

Author name: جعفر انور قدوري الخاجي
Supervisor name: رباب عمران راضي الجيلاوي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: Fifteen local isolates of Bacillus bacteria and previously isolated were screening a from laboratory of advanced biotechnology and Genetic Engineering in Babylon University, College of Science to know their ability on production of extracellular alkaline phosphatase after its growth in liquid production in PH8 medium for 4 days and temperature 37°C. and destinguished 11 isolates by their different abilities on produc of enzyme and Bacillus sp.I bacteria was their best and perfected it selection to complete the study and described by depending on microscopic and cultured and biochemical characteristics studying of effect some of environmental condition for production of alkaline phosphatase enzyme by submerged cultural method were appeared that the optimum PH for production was 8, and optimum temperature 40°C, after incubation time 4days and explain that the production by static incubator was better from shaker incubator.Alkaline phosphatase of the isolate Bacillus sp.I was purified partial by fractionating with ammonium sulphate and the best ratio for saturation 95%. And on purification of enzyme by ion exchange chromatography with batch wise by using DEAE - cellulose and enzymatic recovery was 37.1%, and fold 3.64 once, and enzyme passed after concentrated on gel filtration column (Sepharcyl S - 200) one peak was appeared for enzyme and enzymatic recovery was 23 % and fold 36.80 once.Results of electrophoresis by presense of denaturation condition appeared that the molecular weight of alkaline phosphatase produced from gel filteration by using Sepharcyl S - 200 gain approximately 29.5 KDa and 28.8 KDa and this enzyme composed of homodimer.The characterization results for the enzyme indicated that the optimal pH for activity of enzyme was (9), while the optimal pH for the enzyme stability was (8 - 9), and found that the optimal temperature for activity of anzyme was 30 ? C, and on studying optimal temperature for stability of enzyme indicated that better stability for this enzyme was on temperature 0 ?C while the activity lost completely on temperature 60°C. and on studying effect some chemical compounds in activity of enzyme found that the enzyme needs to cofactor and it is zinc andmagnessium ion to increase catalytic activity and needs to calessium ion to increase stability of enzyme. At that time potassium and sodium ions appeared a negatively effect on activity, and heavy metals ions Fe and Hg appeared an inhibited effect to activity and so that the enzyme was inhibited by presence EDTA and and potassium ferro cyanide indicating that the enzyme from metal enzymes.Determination of constant kinitics of the enzyme was completed of Michaelis - Menten constant (Km) of para - nitrophenyl phosphate and this gained 500 micromolar and maximum velocity of this enzyme (Vmax), and this gained 2500 unit/ml.

دراسة مناعية لمستضد متعدد السكريد للفطر Aspergillus niger في الارنب == Immunological Study of Aspergillus Niger Polysaccharide Antigen In Rabbit

Author name: غدير كاظم عبود الخفاجي
Supervisor name: نداء شهاب حمد | فريال جميل عبد
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: جمعت خلال هذه الدراسة 50 عينة تمثلت ب 25 عينة تربة و25 عينة قشع من اشخاص مصابين بالتدرن الرئوي والوافدين لمركز التدرن والامراض الصدرية في محافظة بابل خلال المدة المحصورة للفترة من تشرين اول 2013 ولغاية شباط 2014 تم خلالها عزل الفطر Aspergillus niger وبنس | fifty specimens were collected, 25 samples were collected from soil while the other 25 sample were collected from sputum of pateints with Tuberculosis, who admitted to thoracic diseases center in Babylon province in period October 2013 to Febreury 2014. Aspergillus niger Has been Isolated from these sample with 56% appearance in soil & 48% in sptum of TB pateints.these isolated were examined micro and macroscopically. purified Fungi was culture on potato dextrose broth, after incubation period polysaccharide antigen of A.niger cell wall was prepared for isolated from soil and TB patients suptum. Molish, protein and glucose test to provide that these antigen are polysaccharide, then immunuization of local rabbit of Orcyctalagus conninculus age 3 - 5 months & 1 - 1.5 Kg for males only (3 animals for each antigen) After the immunization period (one month) some of local and systemic immune response parameters (cellular and humoral) to test animals were studied and compared with control animals which immunized with normal saline. The results of study showed that the polysaccharide antigen isolated from these two strains lead to stimulate systemic humaral immune response which had been studied by using direct agglutination test for appendix immunoglobulin passive haemagglutination test (both were used to determined antibody titer), radialimmunodiffusion test in order to determine level of concentration of immunoglobulins (IgG, IgM) and complement part (C3, C4). where The results of direct agglutination titer of animals immunized with polysaccharide antigen 128 and 1 for control animals and heamagglutination tests titer of animals immunized with polysaccharide antigen serum (2560). These results were higher compared with control group titer (10) and this is also true for immunoglobulin and concentration of IgG, IgM for immunized animals compared to control one. In addition to that The concentration of C3 and C4 had been increased in immunized animals compared to the control. cellular immunological response had been measured by using rabbit Neutrophil Elastase Kit. The eresult show an increase in the level of phagocytosis in animals immunoized with polysaccharide antigen isolated from A.niger cell wall of TB pateints 0.37±0.03 pg/dl while decreased in animals immunoized with polysaccharide of soil A.niger 0.72294±0.25548pg/dl compared with control animals 0.6±0.16386 pg/dl. on the other hand the humaral adaptive immune response had been identified by measuring cytokine IL - 2, IL - 10 in serum rabbit, the level of IL - 2 in rabbit immunoized with polysaccharide antigen soure TB patients and soil is 5.4129±1.08979 and 4.2641±0.62997 pg/dl respectively compared with control animals 3.9327±0.55587 pg/dl. The level of IL - 10 had been dropped for immunized animals with A.niger of TB pateints and from soil 4.8762±1.15104 and 8.2632±4.31933 pg/dl while the control animals having the highest level 10.2576±5.23356 pg/dl among the groups. IgE concentration measured significant difference at p>0.05 in immunized animals of A.niger in both TB pateints and soil 1.1701±0.05811 and 1.0770±0.19355 ml/Iu compared with control where IgE0.8723±0.0849ml/Iu. Miotic index of bone morrow immunized and control animals show mean difference is signification at p>0.05.where the result demonstrate that the polysaccharide antigen fissed the bone marro cells compared to controlled one. In order to identify the viability of antigen in protection. challenge dose test made by injection animals with 108 spore/ml of A.niger conidia which prove their protection in immunized animals while the control animals undergo many morphological and behavioral changes after 5 - 7 days of their injection.I addition that the size of lung and spleen increased when compared with immunized one. The histological culture of lung and spleen of challenge test animals in order to identify the ability and speed of their body to evadicate and prevent infection, display that A.niger coloni appeared for control animals (6 rabbit) while disappeared in culture of immunized one. Skin test also performed to experimental animals which indicate that polysaccharide antigen of A.niger interact with hypersensitivity test of rabbit, where the level of late hypersenetivity increased clearly with propability of p>0.05 with the highest level after 24 hours of beginning test compared to controlled one.

دراسة بيئية وحياتية للمجتمع السمكي في نهر الفرات/ وسط العراق == Ecological and Biological Study of fish Community in Euphrates River / Middle of Iraq

Author name: ماهر محمد خضيري
Supervisor name: ميسون مهدي صالح الطائي | مؤيد جاسم ياس العماري
General topic: Biology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: اجريت الدراسة الحالية في نهر الفرات عند سدة الهندية خلال المدة من تشرين الاول 2012 لغاية ايلول 2013 لغرض دراسة بيئة وحياتية المجتمع السمكي، وتم اختيار موقعين، يقع الموقع الاول في شمال سدة الهندية، ويقع الموقع الثاني في جنوب سدة الهندية، وتضمنت الدراسة تاث | The Present study was carried out on the Euphrates River during the period from October 2012 to September 2013 to study ecology and biology of fish community in the river, two study sites were chosen the first one was in the north and the second one was in the south of Al - Hindiya dam.The study was included the effects of some physical, chemical factors of water and Al - Hindiya dam on ecological and biological of fish population as well as measurement of the concentrations of some heavy metals (Pb, Cd, Mn, Zn) in both dissolved and particulate phases of water and in the tissue of three types of freshwater fish that was high dominance in three age groups. Study of the number of species, individuals, Total of weight of individual. Some ecological indices were study as index of Relative abundance, Species Richness index and Shanon wiener index for determining the evaluate of biodiversity of fish. Also Jaccared index of similarity was used to explain the similarity between sites of study and among months. Constant index was used to determine the constancy of taxa. Biological study of this of three fish that have high dominance from the side of food, age, growth, sex ratio, gonado somatic index and length - weight relationships. Air temperature and water temperature were ranged in two study sites between (15 - 43.8) C? and (10 - 29) C? respectively, pH values were between (7.6 - 8.6), Water current velocity values were between (0.14 - 0.72) m/sec, light penetration values were between (27.3 - 193.3) cm, the value of electrical conductivity were between (822 - 1323) ? sem/cm , Total dissolved solid values were between (663.3 - 932) mg/L, while The total suspended solid were between (0.009 - 0.23) mg/L and the values of dissolved oxygen were ranged between (6.13 - 10.33) mg/L.The biological oxygen demand were recorded values that range between (0.68 - 4.3) mg/L. . The water of Euphrates river were considered slight alkaline according to the results of present study and ranged between (33.33 - 164.3) mg CaCO3/L and the water were very hard according to high values record for total hardness (391.6 - 886.6) mg CaCO3 /L, while the values of calcium and magnesium hardness were ranged between (86.1 - 188.4), (19.8 - 138) mg CaCO3/L respectively.The values of phosphate were ranged between (0.51 - 13.12) ?g/L and Nitrate values were ranged between (6.23 - 70.17) ?g/L, while the values of nitrite were ranged between (0.17 - 4.98) ?g/L. in especially, the effects of Al - Hindiya dam, the results were showed that numbers of species before and after of Al - Hindiya dam were ranged between (23 - 17) species respectively. the results were showed positive and negative correlation between physical, chemical properties of water and Heavy metals with number of species, individuals, weights and Biodiversity indices. This study showed that the mean concentrations of the heavy metals (Pb, Cd, Mn, Zn) of the dissolved phase in water were (2.14, 1.61, 3.10, 7.63) ?g/L respectively and in the particulate phase were (33.99 112.87، 84.21، 21.12،) ?g/g dry weight respectively, the results of present study were showed that the concentration of heavy metals in water in dissolved and particulate phases within local and world limitations. The heavy metals in muscular tissue for three groups of this fish were ordered in as following : Zn >Mn > Pb > Cd. Twenty - three fish species were collected, (17) of them belonged to Cyprinidae family and two species belonged Bagridae family while only one species represented each family of Mugilidae, Siluridae, Mastacembelidae, Cichlidae, the number of species were ranged during the period of study between (5 - 18), The total number of individuals was 2401 individual and the number of individual were ranged monthly between (129 - 276) individual, while the total weight of individuals were 232868 gm during the period of study and The weight of individuals were ranged monthly between (13072 - 29598) gm. Carassius auratus wae recorded with the highest percentage in number and weight as 35.2% and 27.5% respectively. while A.vorax was after C.auratus in total number 13.6%, but it was after C.auratus and C.carpio in total weight 16.9% of the total individual weight. The values of species richness index were ranged between (0.79 - 3.23) and numerical of shanon wiener index values (Hn) were ranged between (1.26 - 2.39), while the weight of shanon wiener index value (Hb) were ranged between (1.21 - 2.35). the numerical and weight of eveness index (Jn) were ranged between (0.65 - 0.86), (0.68 - 0.89) respectively during the period of study. Jaccared similarity index were ranged between (23% - 92%). And the study was revealed that the most species recorded during the period of study were constant species according to constancy index. C. carpio fish were Omniovorous and It's feeding activity was ranged between (70% - 91.42%) and feeding intensity was (6.9 - 12.17) degree/fish.while C. auratus were detritious, It's feeding activity was ranged between (56.75% - 94.28%) and feeding intensity was (6.91 - 12.87) degree/fish. A. vorax were carnivorous and It's feeding activity values were ranged between (69.2% - 90.32%), while feeding intensity values were ranged between (6.85 - 13.03) degree/fish. The results was showed the values of age and length for C. carpio that ranged between (1 - 5) years, (9.7 - 40.2) cm , and C. auratus were ranged between (1 - 6) years, (8.3 - 28) cm, while A.vorax were ranged between (1 - 7) years, (10 - 62) cm respectively. Sex ratio and gonado - somatic index (GSI) varied between the three species, sex ratio of C.carpio (male : female) was 1 : 3.1. (GSI) for male was ranged between (2.21 - 12.02) and for female between (3.36 - 19.36).The sex ratio of C. auratus male : female was 1 : 1.9, GSI values for male was ranged between (0.89 - 8.53) and for females between (1.23 - 61.37). A.vorax sex ratio male : female 1 : 1.5, GSI value for male and female were ranged between (0.36 - 2.84) and (0.24 - 5.83) respectively.Variation of values of slope factor (b) were calculated from length - weight relationships for three species of fish, the values of (b) factor for C.carpio, C.auratus and A. vorax (3.08, 2.97, 2.92) respectively during the period of study

الكشف عن الجينات المقاومة للكاربابنيم لبكتريا Acinetobacter baumannii المعزولة من عينات سريرية في محافظة بابل == Detection of Carbapenem - Resistant Genes of Acinetobacter Baumannii Isolated From Clinical Samples In Babylon Province

Author name: رعـد عبد العباس حمزة الهرموش
Supervisor name: ايمان محمد جار الله
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: للفترة من اذار 2014 الى اذار 2015 تم جمع 1300 عينة سريرية مختلفة (عينات ادرار ومسحات جروح وحروق وعينات دم ومسحات من الفم والاذن والعيون بالاضافة لعينات البراز والقشع) من اثنين من مستشفيات محافظة بابل (مستشفى الحلة التعليمي ومستشفى بابل التعليمي للنسائية و| During the period between March 2014 and March 2015, a total of 1300 clinical specimens (urine, wounds, burns, blood, throat, ear, eye, stool, and sputum) were collected from two hospitals in Babylon province; Al - Hillah Teaching Hospital, and Babylon Hospital for Maternity and Pediatric. All specimens were cultured and 10 Acinetobacter baumannii isolates were obtained from clinical specimens in a percentage of (0.76%) distributed in blood 1 (0.6%), urine 2 (1%), wound infection 1 (0.75%), burn 5 (0.85%) and throat 1 (2%). This study was conducted to determine the occurrence of carbapenem resistant A. baumannii isolates obtained from Hillah hospitals. Isolates were identified according to API20E system and further confirmed using PCR technique. Antibiotics susceptibility was assayed by using disks diffusion method according to CLSI recommendations. All isolates revealed positive results with rapid iodometric test indicting the ability of beta lactamase production. Phenotypic detection of carbapenemase production was performed using the imipenem - EDTA disk and modified Hodeg's test (MHT). Then isolates were subjected to monoplex PCR targeting blaOXA - 51, blaOXA - 23, blaOXA - 24, blaOXA - 58, blaIMP, blaSIM blaNDM - 1, blaNDM - 2 and blaVIM genes, as well as, efflux pumps genes. One of the identified isolates (10%) was found to be imipenem, meropenem and ertapenem resistant, this isolate gave positive result with the imipenem - EDTA disk and (60%) of isolates were positive to MHT. PCR experiments showed ; all isolates were harbored blaOXA - 51 gene, four (40%) isolates were harbored blaOXA - 23 gene, three (30%) isolates were harbored blaOXA - 24 gene, four (40%) isolates were harbored blaOXA - 58 gene, five (50%) isolates were harbored blaIMP gene, six (60%) isolates were harbored blaSIM gene, two (20%) isolates were harbored blaNDM - 1 gene, four (40%) isolates were harbored blaNDM - 2 gene, while none of these isolates harbored blaVIM gene. The present findings suggest that emergence of (OXA - 23, OXA - 24, OXA - 58, IMP, SIM, NDM - 1, NDM - 2) carbapenemase producing A. baumannii clinical isolates in Hillah City hospitals. Also there are indicative appearance of efflux pump genes ; (50%) for Ade - A and Ade - B each other, (40%) for Ade - C, (80%) for all of Ade - R and Ade - S, (100%) for all of Ade - I and Ade - J, and (50%) for Ade - Y. All isolates of A. baumannii appear as MDR, while only one appear to be as PDR. The trans - mobility of resistant genes was examined by trans - conjugation experiment ; the results indicated that only blaIMP and blaOXA - 23 genes were transferred horizontally in the current study. In attempting to investigate any blaNDM gene variation the results showed some different than NCBI - Blast A. baumannii blaNDM - 1 and blaNDM - 2 it may as a unique genotyping.These results revealed that carbapenemase producing A. baumannii were detected in both phenotypic and genotypic methods (PCR). This underlies the importance of their accurate identifications and reporting to prevent the emergence of complete resistance to the most potent drugs against A. baumannii in Babylon province.

دراسة جزيئية، وخلوية وراثية، ومناعية لمرض سرطان القولون والمستقيم في العراق == Molecular, Cytogenetic And Immunological Study of Colorectal Cancer In Iraqi Patients

Author name: حيدر جاسم محمد الخفاجي
Supervisor name: علي حمود السعدي | عزام قنبر اغا
General topic: Biology
Specific topic: Zoology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: The study consists of three parts related with patients of CRC as in : 1 - Molecular part 2 - Cytogenetic and molecular genetics part and 3 - Immunological part. In addition of their relatives of clinical and pathological diagnosis, genus, and tumor locations in 150 of Iraqi patients confirmed with CRC collected from Teaching Hospitals of some Iraqi governorates. The percent of male to female (54.66% / 45.43%) and the aged range of study were 16 to 80 years.The first part consist a study of five types of genes related with CRC represented by MLH1, MSH2, APC, K ras, and SMAD7 genes. Some of these genes develop with germline mutations as in MLH 1 exons1 and 15 so as MSH2 genes. While the rest genes related with a sporadic of CRC. All these genes were amplified by conventional polymerase chain reaction (PCR) for study the types of the mutations and their defect of these genes on CRC. The APC exon11and K ras exon1 genes were processed by single strand conformational polymorphism (SSCP) technique. The results of PCR - SSCP technique for two genes represented by appeared three types of bands, which were (AA), (AB) and (AC) bands. The important band was (AB) which constitutes the variability region represented by 84.2% and 44.82% in patients for both genes. The results of patients with mutated bands with high significant differences(P?0.05).compared with healthy control. Patients carried these bands subjected to PCR - Sequences technique. The analysis results for APC gene sequences were substitutions mutations type of single nucleotide polymorphism (SNP) change Cytocine to Thiamin (C>T) at 1972 location of genome. While the analysis results of Kras gene appeared two types of mutations, substitution and frame shifts represented by deletion mutation and so as appeared stop codon mutation.The other genes represented by MLH1, MSH2, and SMAD detected by direct PCR - Sequences. The results analysis of MLH1 gene, exon (1and 15) represented by nucleotides change with high frequencies of substitution mutation through exon1 at different locations of nucleotides. So as frame shift mutation type deletion through exon15. While for MSH2 gene, exon 6 from 6.66% represent by deletion mutation. A spread study for SMAD7 gene, exon4 were done through 30 patients average age 52 years (male to female 56.7% : 53.3%) confirmed colorectal adenocarcinoma compared with 15 healthy. A mutation of 20% of (SNPs) were identified.The second part which represented standard conventional cytogenetic and molecular genetics were done through peripheral blood culture (PBC). The study showed increasing of mitotic index (MI) in patients with significant differences (P?0.05) compared with healthy controls indicating for increasing of proliferation of the lymphocytes division. The study showed some chromosomal aberrations with significant, the majority of aberrations were higher significant in dwarf and aneuploidy chromosomes so as elongation chromosomes revealed significant differences (P?0.05) between patients and healthy. The appeared of Polyploidy chromosomes, with significant differences (P?0.05), that’s seem to be good indicator for cancer disease. The molecular genetics alterations which dependent on cytogenetic investigation were done through deletion of long arm of 5 chromosome which APC gene is located. The test elucidates the importance of inactivation (deletion) of APC gene in elicited of colorectal cancer. The study of 20 patients, were investigated by fluorescent in situ hybridization (FISH). The results showed 60% patients which have 5q arm deletion of (+). And (5%) have two deletions (++) which was a male aged less than 60 years. The results analysis explained significant differences (p?0.01) between studied group and deletion (+) of arm 5q. A correlation represented by significant differences (p?0.01)through dysfunction of APC gene deletion of 5q were found with high percent among sporadic adenocarcinoma 58.3%, with less in attenuated familial adenomatous polyposis (AFAP) and familial adenomatous polyposis (FAP) represented by the (25%), and (16.7) respectively.The last part related with immunological test were done by detection of tumor markers represented by Carcino Embryonic Antigen (CEA), IL - 33 and IL - 31.These markers represented a confirmative indicators for colorectal adenocarcinoma which were tested by ELISA technique in (79) patients confirmed with CRC of male and female. These tumor markers in tested with patients showed high significant differences (p?0.01) compared with healthy controls. Another immunological test for detection of Human papilloma virus (HPV) type 18 E6 gene expression in CRC by Immune histochemistry technique (IHC) in 71 (FFPET) of CRC. The results of this study showed there were a correlations between patients and adenocarcinoma positive HPV18 E6 infection 43.7% of patients infected with virus, with high significant differences (p?0.01) and higher infection in female (67.7%) than male (32.3%). So as a correlations were found between HPV18 E6 expression and tumor tissue locations, explained by high percentage 45.2% in rectum.While a tumor marker was (CD8+) was used to determine its role in human immune surveillance in tumor regions of CRC so as with regions which were infected by HPV. The study revealed high significant differences between healthy control and studied groups which have (CD8+) positive infiltration in tumor origins through detection by (IHC).In conclusions diagnosis of CRC can be detected by special genes like K ras gene through professional PCR processing, compact with tumor markers, so as Fluorescent in situ hybridization (FISH) technique provides a precise method which can be used for detection of alteration of molecular and cytogenetic related with CRC.

النمو، الفعالية الانزيمية والتعبير الجيني للانزيمات المحللة للبروتين (Protease) في الفطر الجلدي Trichophyton rubrum == Growth, Enzyme Activity And Gene Expression of Proteases In A Dermatophyte Trichophyton Rubrum

Author name: سارة كريم كاظم الزبيدي
Supervisor name: جواد كاظم الجنابي | عدنان حمد الحمداني
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: صممت الدراسة الحالية لتوصيف الانماط الجزيئية والمظهرية للنمو والفعالية الانزيمية للانزيم المحلل للبروتين والتنوع الوراثي وعلاقته بالتعبير الجيني بين عزلات الفطر الجلدي.Trichophyto rubrum ولهذا الغرض جمعت 150 عينة سريرية (قشطات جلدية (80), اجزاء شعر (60) | The present study was designed to characterize the morphological and the molecular patterns of growth, enzymic activity of proteases, genetic diversity and their correlation with gene expression among strains of Trichophyton rubrum. For these purpose, A total of 150 clinical specimens (skin scrapings (80), hair fragments (60) and nail clippings (10)) were collected from 100 patients (70 males&80 females) whom clinically diagnosed with dermatophytosis after attendingto the dermatology and venereal diseases centre of Mergan Teaching hospital and private clinic in Babylon city from Feb. 2014 to May 2014. T. rubrum were isolated and identified using cultural, biochemical and physiological tests. Isolates were then subjected for confirmation by PCR, genotyping using RFLP - PCR, sequencing and registration of these sequences in GeneBank for obtaining accession numbers then phylogeny. The growth conditions, were tested, in addition to, the genetic expression of proteases (exocellular protease and endocellular aminopeptidase) were determined using Real - Time PCR. Out of 150 specimens, 24 (16%) isolates were dermatophytic fungi and only 5 (20.8%) of them belong to T. rubrum. The influence of cultural conditions in growth of T. rubrum showed that the 30 ?C, pH 6, SDA medium and 7 days incubation were the optimal conditions for its growth. Proteolytic activity of isolates of T. rubrum against casein as a substrate revealed an ability to produce protease in solid and liquid media after 14 days of incubation. This activity was varies according to the type of isolate where the isolate No.1 (isolated from skin) gave a high proteolytic activity (5.6cm) in solid media and (80.1U/ml) in liquid media in comparison with other tested isolates (No.2 - No.8). on the other hands, the 9 days of incubation, 30 ?C, pH 7 and 0.5% substrate concentration were the optimal condition for proteolytic activity of these isolates. The molecular tests confirmed that all tested isolates belong to T. rubrum with amplicon size (601bp) after amplification of ITS1 primers using PCR technique. While the RFLP - PCR technique showed the presence of two genotypes (I%II) belong to T. rubrum with subgenotypes (Ia - Id) and (IIa - IId) respectively. The relative quantification of proteolytic activity (exocellular protease and endocellular aminopeptidase) produced by T. rubrum genotypes were expressed by using Real - Time PCR after amplification of the target gene of ptotease and aminopeptidase in comparison with housekeeping gene (? - actin) as a reference gene. The results showed the up - regulation of gene encoded to exocellular protease than the down - regulation of endocellular aminopeptidase produced by T. rubrum in the presence of casein as a substrate. The internal transcribed spacer 1, partial sequence, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence and 28S ribosomal RNA gene, partial sequence of local isolates of T. rubrum were used for sequencing, registration in Genebank - NCBI and phylogeny. Five accession numbers were recorded and available to NCBI, EMBL in Europe and the DNA Bank of Japan. These accession numbers were : KP979787, KP979788, KP979789, KP979790, KP979791. Phylogenetic relation between local strains and world strain showed a high identicasl with T. rubrum (GQ376105.1). This is the first study in Iraq which employed sequencing, registration of sequences in Genebank - NCBI and carrying out phylogeny of local and world strains of T. rubrum.

مستوى الهستامين وبعض المعايير المناعية لمرضى الحساسية التنفسية من النوع الاول == The Level of Histamine And Some Immunological Parameters Among Airway Hypersensitivity Type I Patients

Author name: سلوى احمد حمزة الكرادي
Supervisor name: فريال جميل عبد
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: تم جمع 50 عينة دم من مرضى الحساسية التنفسية من النوع الاول المراجعين لمركز الحساسية والربو في محافظة بابل للفترة الممتدة ما بين تشرين الثاني 2013 ولغاية شباط 2014, بالاضافة الى 10 عينات دم من الاشخاص غير المصابين بالحساسية من النوع الاول والذين كان معدل I | Blood samples have been drawn from 50 airway hypersensitivity type I patients who visit the center of the allergies and asthma in the province of Babylon, for the period between November 2013 and up to February 2014, in addition to 10 samples from persons with normal IgE level as a control. The sample was divided into two parts, the first used to measure the level of eosinophils and basophils and the second used to measure the level of immune parameters, which included histamine, IgE, IL - 5, IL - 10 and IL - 12 by using the technique of Enzyme - Linked Immunosorbant Assay (ELISA). Study community were divided into three groups, the first group named before immunotherapy treatment and the second group after immunotherapy treatment and final is for control. Conducted differences in the level of immune parameters between the three groups, there were significant differences between the group before the treatment and control group as well as between the group before treatment and after treatment. The level of histamine for the three groups (before treatment and after the treatment and control), reached respectively 34.8292 ± 6.47680 ng / ml and 9.3238 ± 3.55296 ng / ml and 6.9844 ± 3.05477 ng / ml, and the level of IgE reached to the before treatment group 40.6279 ± 15.24231 IU / ml and the after treatment group 23.9529 ± 11.88933 IU / ml and control group 11.7796 ± 5.53 IU / ml, while the cell count of eosinophils for the before treatment group 0.7283 ? 0.24556 x 103 cells / mm 3 and after treatment group 0.3643 ? 0.14861 x 103 cells / mm 3 and control 0.2252 ? 0.09139 x 103 cells / mm 3 , as well as for the cells of basophils for the before treatment group 0.1008 ? 0.03391 x 103 cells / mm 3 and after treatment group 0.0777 ? 0.03485 x 103 cells / mm 3 and control 0.0724 ? 0.03568 x 103 cells / mm 3 , As for the cytokines the level of IL - 5 reached for the same groups 51.3214 ± 6.18565 pg / ml and 22.7254 ± 3.99360 pg / ml and 24.5404 ± 8.64920 pg / ml respectively, while IL - 10 has reached 14.9604 ± 6.48170 pg / ml and 43.0476 ± 21.26668 pg / ml and 24.4969 ± 10.21066 pg / ml, respectively, and finally the level of IL - 12 reached for the same groups 17.753 ± 8.39343 pg / ml and 39.1929 ± 17.73975 pg / ml and 26.7731 ± 12.93092 pg / ml, respectively. There were been a difference in the level of immune parameters for a number of variables, , place of residence, gender, and type of allergy, there were no significant differences observed in the level of immune parameters between these variables. comparison was made in the level of immune parameters for the group after the treatment depending on the duration of immunotherapy, and found that there were significant differences in the level of these parameters with the increase of the duration of treatment. Through this study, conducted the correlation among histamine and the rest of the immune parameters and the results were pointing to the positive correlation of histamine with IgE, IL - 5, eosinophils and basophils and negative correlation with IL - 10 and IL - 12.
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