Abstract: حالة الحمل هي متعلقة بالتغيرات الفيزيولوجيه والتي تساعد على بقاء الجنين والتحضير للولادة .تقسم مده الحمل الى ثلاث مراحل كل مرحله تشمل ثلاث اشهر من خلالها توصف التغيرات التي تحصل اثناء فتره الحمل وهي مهمة في معرفه التغيرات الطبيعية التي تحصل اثناء الحمل لتساعد في تشخيص المشاكل الطبية للحامل مثل زيادة في نشاط الغدة الدرقية او سكر الحمل او فقر الدم والتغيرات الكيميائية الحياتية عند الحامل تعكس بصوره واضحة وممتازة عن حاله غير الحمل عند النساء ،عدة هرمونات تفرز اثناء الحمل قسم منها يختفي بعد الولادة والاخر يتغير في مستواه حتى بعد الولادة ،هرمونات الغدة الدرقية وظائفها اثناء الحمل تتغير خاصة خلال الاشهر الثلاث الاولى حيث يعتمد الجنين بصوره كلية على هرمونات الدرقية للام وهي تؤثر على عمليات ايض الكاربوهيدرات والبروتينات والدهون ، فتقل حساسية الانسجه للاستجابة للانسولين مما يودي الى انخفاض مستوى الكلوكوز عند الام ناتج سكر الحمل وكذالك فقر الذي ياتي نتيجة الزيادة الحاصلة في حجم الدم الى حوالي 40% وهذا ايضا يسبب نقص في مستوى الالبومين ايضا التغيرات الهرمونية والتغيرات الكيمياويه تظهر الاختلاف الواضح بين حاله الحمل وغير الحمل .طريقه العمل : في هذه الدراسة تم دراسة مصل 50 امراة حامل خلال الثلاث فترات من اشهر الحمل تراوحت اعمارهن بين (16 - 50 )سنه ومصل 20 امراة متزوجة غير حامل تراوحت اعمارهن بين (17 - 54 )سنه اعتبرت كمجموعه سيطرة. تم اخذ النماذج من مستشفى العلوية للولادة ومختبر الصحة المركزي وخلال الفترة من 1/7/2007 الى 8/4/2008 . حيث تم قياس هرمونات الغدة الدرقية وهي( T3,T4,TSH) بواسطة استخدام الفلوره المناعية باستخدام جهاز miniVidus في مصل النساء الحوامل وخلال الثلاث مراحل من فتره الحمل , وكذالك تم قياس مستوى الكلوكوز وحامض اليوريك والبروتين الكلي والكوليستيرول والترايكليسرايد والبروتينات الناقلة للدهون وهي البروتين الناقل عالي الكثافة HDL والبروتين الناقل قليل الكثافة LDL والبروتين الناقل ذو الكثافة الواطئة جدا VLDLونسبه الكوليستيرول/البروتين عالي الكثافة وكذالك نسبه البروتين منخفض الكثافة /البروتين عالي الكثافة وايضا الهيموكلوبين تم قياس النماذج بواسطة جهاز , ,spectrophotometer في مصل كل من النساء الحوامل وغير الحوامل .الهدف من الدراسة : 1 - قياس مستوى هرمونات الدرقية T3,T4,TSH) ) خلال الاشهر الثلاث الاولى والاشهر الثلاث الثانية والاشهر الثلاث الثالثة عند النساء الحوامل وكذالك عند النساء غير الحوامل.2 - قياس المتغيرات الكيميائية الحياتية وتشمل الكلوكوز وحامض اليوريك والبروتين الكلي والكوليستيرول والترايكليسرايد والبروتينات الناقلة للدهون وهي البروتين الناقل عالي الكثافة HDL والبروتين الناقل قليل الكثافة LDL والبروتين الناقل ذو الكثافة الواطئة جدا VLDLونسبه الكوليستيرول/البروتين عالي الكثافة وكذالك نسبه البروتين منخفض الكثافة /البروتين عالي الكثافة وايضا الهيموكلوبين عند النساء الحوامل وغير الحوامل .3 - معرفه العلاقة بين هرمونات الغدة الدرقية والمتغيرات الكيميائية الحياتية عند الحوامل وخلال الثلاث فترات من الحمل وكذالك القياس عند النساء الغير الحوامل.النتائج : ا - في الاشهر الثلاث الاولى كان هناك ارتفاع في مستوى هرموني الدرقية T3,T4 ونقصان في مستوى هرمون TSH. خلال الثلاث فترات من الحمل ويرجع هذا الى اختلاف في تركيز البروتينات الحامله (Thyroxin Binding Globulin and Prealbumin - TBG) جميع المتغيرات كانت ذات مستوى منخفض عدا مستوى الHDL كان عالي اما مستوى البروتين الكلي لم يعاني تغير خلال هذه الفترة .ايضا وجد T4 كانت له احتماليه عاليه جدا (P<0.001) بينما T3,TSH لم تكن لهما احتماليه . كذالك الكلوكوز والهيموكلوبين كانت لهم احتماليه اقل من (P<0.05) والباقي لم تظهر احتماليه. ايضا يعتبر الكلوكوز هو مصدر الطاقة للجنين والذي ينتقل عبر المشيمة عن طريق الانتشار السهل لذالك يزداد افراز الانسولين خلال الحمل نتيجة نقصان في مستوى الكلوكوزوهذا يؤدي الى استخدام الجنين كميه اكبر من الاحماض الدهنيه،خلال فتره الحمل زيادة معدل الفلتره يرتفع معدل التصفية (GFR) الى حوالي 50% ونقص في القدره على طرح الماء يؤدي الى الاستقاء في الكاحل والساق وهذا يؤدي الى زيادة في مستوى حامض اليوريك خلال الاشهر الاخيره من الحمل ،يزداد حجم الدم الى حوالي 40 - 50% وتبدا هذه الزيادة في الثلث الاول من الحمل وتزيد بسرعة في الثلث الثاني ثم تستقر في الاسبوع الثلاثين من الحمل ،هذه الزيادة ضرورية لامداد كميه اضافيه من الدم للرحم ،والاحتياجات الايضيه للجنين وزيادة نشاط الاعضاء الاخرى وخاصة الكلى وهذه الزيادة في حجم الدم ينتج عنها انخفاض في تركيز الهيموكلوبين ،الالبومين،وبروتينات البلازما الاخرى.وجد هنالك علاقة موجبه قويه بين هرمون TSH مع ,uric acid ,cholesterol, VLDL , glucose. ايضا وجدت علاقة سالبه قويه بين T4 مع glucose . هنالك علاقة موجبه قويه بين الكلوكوز وحامض اليوريك في نفس الوقت علاقتهما مع الكوليستيرول وعلاقة الثلاثة مع البروتين الدهني واطئ الكثافة. في حين الترايكيسرايد له علاقة مع حامض اليوريك والبروتين الدهني واطئ الكثافة جدا.الهيموكلوبين له علاقة موجبه قويه مع البروتين عالي الكثافة . ب‌ - في الاشهر الثلاث الثانية كانت هنالك ارتفاع مستوى هرموني الدرقية T3,T4 ونقصان في مستوى هرمون TSH . الكلوكوز ارتفع عن مستوى الشهر الاول لكن مازال منخفض عن مجموعه السيطرة ايضا حامض اليوريك والبروتين الكلي والترايكليسرايد والبروتين الدهني واطئ الكثافة جدا والهيموكلوبين كانت ذا مستوى منخفض في حين الكوليستيرول والبروتين عالي والواطئ الكثافة كانت عاليه المستوى . ايضا وجد T3,T4 لهم احتماليه عاليه جدا (P<0.001 ) في حين TSH له احتماليه اقل من (P<0.05) , ايضا الكوليستيرول والبروتين واطئ الكثافة والهيموكلوبين اظهرت احتماليه اقل من (P<0.05) وجد هنالك علاقة موجبه قويه بين هرمون TSH مع uric acid , glucoseوايضا بين T4 وT3. وعلاقة بين T3 والتراي كليسرايد وكذالك مع البروتين الدهني الواطئ الكثافة جدا وجد ايضا علاقة سالبه قويه بين TSHوالتراي كليسرايد . ايضا هنالك علاقة موجبه قويه بين الترايكيسرايد والكوليستيرول . البروتين واطئ الكثافة له علاقة مع الكوليستيرول . البروتين واطئ الكثافة جدا مع الكوليستيرول والتراي كليسرايد . الهيموكلوبين له علاقة مع الكلوكوز . ايضا هنالك علاقة سالبه قويه بين الترايكليسرايد والكلوكوز.ج‌ - في الاشهر الثلاث الثالثة كانت هنالك ارتفاع مستوى هرموني الدرقية T3,T4 ونقصان في مستوى هرمون TSH . مستوى الكلوكوز والبروتين الكلي منخفض في حين باقي المتغيرات كانت مرتفعه المستوى مقارنه بمجموعه السيطرة . ايضا وجدT4 له احتماليه عاليه جدا (P<0.001 ) في حين T3 له احتماليه اقل من (P<0.05) ,في حين الTSH لم يظهر احتماليه. ايضا الكوليستيرول والبروتين واطئ الكثافة والترايكليسرايد لهم احتماليه عاليه جدا(P<0.001) في حين البروتين الكلي والبروتين الدهني الواطئ الكثافة جدا اظهرت احتماليه اقل من (P<0.05) . وجد هنالك علاقة موجبه قويه بين هرمون TSH مع .glucoseوعلاقه بين T3 والكلوكوز وحامض اليوريك. الكوليستيرول مع البروتين الكلي.الترايكليسرايد مع الكلوكوز. وكذالك البروتين الدهني الواطئ الكثافه جدا مع الكلوكوز والترايكليسرايد. وكذالك البروتين واطئ الكثافه له علاقه مع الكوليستيرول والترايكليسرايد. د - اظهرت النتائج بالنسبه الى النساء الغير حوامل بان هنالك علاقه موجبه قويه بين هرمون ال TSH والبروتين عالي الكثافه وكذالك مع هرمون T3.ايضا هرمون T3 له علاقه موجبه قويه مع البروتين واطئ الكثافه وعلاقه سالبه قويه مع الترايكليسرايد والبروتين واطئ الكثافه جدا. ايضا هنالك علاقه موجبه قويه بين البروتين الكلي مع حامض اليوريك. الترايكليسرايد مع حامض اليوريك والكوليستيرول. البروتين عالي الكثافه له علاقه سالبه قويه مع حامض اليوريك. البروتين واطئ الكثافه جدا علاقه موجبه مع حامض اليوريك والكوليستيرولووالترايكليسرايد. البروتين واطئ الكثافه علاقه موجبه مع الكوليستيرول وعلاقه سالبه مع البروتين عالي الكثافه. الهيموكلوبين مع الكلوكوز علاقه موجبه قويه. | Pregnancy is associated with normal physiological changes that assist fetal survival as well as preparation for labour. It is typically broken into three trimesters, each of about three months. These distinctions are useful in describing the changes that take place over time. Biochemical parameters reflect these adaptive changes are clearly distinct from the non - pregnant state. The mother and the placenta produces many hormones, maternal thyroid function during pregnancy is important for both the mother and the developing fetus. Especially during the first trimester, when the fetus is completely dependent on the mother for thyroid hormone. It also decreases maternal tissue sensitivity to insulin, resulting in gestational diabetes. Dilution anaemia is caused by the rise in plasma volume, Blood volume increases by 40% in the first two trimesters this causes low level of albumin . This is just to an increase in plasma volume through increased aldosterone. Increase liver metabolism is also seen, with increased gluconeogenesis to increase maternal glucose levels .Renal plasma flow increases during pregnancy.Objectives1 - This Study includes measurement of the thyroid hormones T3,T4,TSH and biochemical parameters(glucose, uric acid, total protein ,lipid profile , and hemoglobin) in serum of pregnant women during the three trimesters and serum of non - pregnant women.2 - Investigation the relationship between thyroid hormones level and bio - chemical tests profile in serum of the pregnants during three trimesters and also non - pregnant women.Subject and Methods In the present study the individual sera of hormones (triiodothyronin (T3 ) , thyroxin (T4) and thyroid –stimulating hormone (TSH) were assayed in 70 healthy women . Serum collected from fifty pregnant women at three trimesters and twenty non - pregnant women as a control group. The measurements occurs in both Al - ELWIA Hospital and Central Public Health Laboratories. The T3,T4 and TSH was measured by using Immunophlorences method by miniVidas. This measurement is recruited from 1/7/2007 to 8/4/2008. This study also designed to investigate changes in the levels of biochemical parameters (Glucose, Uric acid ,Total protein, Lipid profile , and Hemoglobin )in sera of pregnant women at three trimesters and on sera of non - pregnant women. The glucose, uric acid, lipid profile were measured by spectrophotometer instrument .The Total protein was measured by using Clinical refractometers instrumental ( ATAGO) and hemoglobin was measured by Micro - Haematocrit Centrifuge instrument ..Results Results of pregnant women at first trimester showed that there was a significant difference (P<0.001) in hormones T4 compared with control group . Results in the second trimester it was found that a highly significant difference (P<0.001)in hormones T3,T4 and TSH (P<0.05) compared with the control group .In the third trimester there was a significant differennce (P<0.001) in hormones T4 and T3 (P<0.05) compared with the control group. The results of Biochemical parameters showed that at the first trimester there was a positive significant difference (P<0.001) in glucose, hemoglobin compared with control group ,In the second trimester there was a positive significant difference (P<0.05) in cholesterol , LDL and hemoglobin , and in the third trimester it was found apositive significant differences (P<0.001) in Total protein, cholesterol , triglyceride , LDL and VLDL compared with the control group. A strong positive correlation coefficient was found between T3 at the firsttrimister with a weak of gestation and HDL, and a negative correlation coefficient between T3 with glucose and uric acid . And a significant positive correlation coefficient was found at the second trimester between T3 with triglyceride, VLDL and CHO/HDL, while a negative correlation with uric acid ,total protein and hemoglobin . A positive correlation was found at third trimester between T3 with glucose and uric acid. Results of non - pregnant group were found with a negative correlation coefficient between T3 with triglyceride, VLDL, LDL ,and LDL/HDL. There was a negative correlation coefficient between T4 at the firsttrimister with glucose . Results At the second trimester there was a positive correlation coefficient between T4 with weak of gestation and T3,there was a negative correlation coefficient between T4 with total protein .Results At third trimester there was a negative correlation coefficient between T4 with triglyceride and VLDL. Results in non - pregnant state there was a negative correlation coefficient between T4 with hemoglubin. There was a positive correlation coefficient between TSH at the firsttrimister with glucose, uric acid, cholesterol, VLDL, triglyceride, LDL, CHO/HDL and LDL/HDL .Results At the second trimester there was a positive correlation coefficient between TSH with glucose, and uric acid ,there was a negative correlation coefficient between TSH with triglyceride, VLDL and T3 . Results At third trimester there was a positive correlation coefficient between TSH with glucose. Results in non - pregnant state there was a positive correlation coefficient between TSH with HDL, and T3 .Conclusions : 1 - In this study it was an increasing in higher T3 and T4 levels and decreasing in the TSH levels in serum of pregnant at the three trimesters which will produced hyperthyroidism if not controlled symptoms.2 - The study proves that changes in biochemical parameters in serum pregnant during three trimester , affects on the mother and fetal. 3 - There were a relationships between thyroid hormone and biochemical parameters for pregnant women and non - pregnant women

تاثير بعض المركبات المشتقة من اليوريا،الثايويوريا وقواعد شف ضد بكتريا Proteus spp. من اخماج المسالك البولية == Effect of Some Synthesized Urea, Thiourea and Schiff Bases Derivatives Against Proteus spp. from Urinary Tract Infections

Author name: كهـلان محمـد عباس الشمري

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Doctorate

University: Al-Nahrain University - College Of Science - Chemistry Department

Language: English

University location: Baghdad

First pages:

Abstract: Novel series of 14 (urea and thiourea) derivative compounds were prepared from reaction of urea and thiourea with aromatic amine compounds (homocyclic and heterocyclic), in addition to preparation of 6 Schiff bases (C=N) compounds that were prepared from reaction of some derived compounds with benzyldehyde in this study. All the 20 prepared compounds were characterized according to F.T.I.R. spectra, purities were examined by T.L.C., and the melting points were measured to identify each of them.From November 2005 through March 2006, a sample of (urine and sera) from 200 patients suffering U.T.I. and urinary stone attending (Diwaniya Teaching Hospital, Pediatrics and Gynecology Teaching Hospital, Health Centers and Private Clinical Laboratories) in Diwaniya, another sample was collected from 100 healthy persons as a control group.The inhibition activity was measured for each of 9 commercially used antibiotics, and for the all prepared compounds in vitro against Proteus bacteria isolated from patients under this study and identified by valid laboratory methods (Api - 20E method).From (renal and bladder) 13 stones were collected from patients to detect the type of each stone by qualitative chemical analysis. Also the level of blood urea, serum uric acid, random blood sugar, serum creatinine and serum calcium, was measured for all samples in this study.This study shows the following results : Bacterial growth for Proteus and other kind of bacteria were found in 138 patients out of the 200 (69 %), while Proteus spp. was isolated from 23 representing by 16.66 % of Proteus spp. out of the 138 patients of U.T.I.. Results of biochemical tests showed that Proteus mirabilis was found in 21 isolates with 91.3 %, and Proteus vulgaris in 2 isolates with 8.7 %.Erythromycin was found to have the lowest inhibitory activity against Proteus spp. isolates with a percentage of resistance 86.9%, while Gentamycin have the highest inhibitory activity with a percentage of resistance 4.3 %. In bacterial sensitivity test among the 9 antibiotics was commonly used in this study.The prepared compounds showed inhibitory activity against growth of isolated Proteus spp. in (19 out of 20) compounds, [N - (5 - phenyl - 1,3,4 - thiadiazole) - 2 - ylthiourae] shows the highest inhibition activity with a diameter of inhibition zone 30 mm., while the [ N - pyridine - 4 - ylurea] shows no such activity in the same concentration, as compared with Gentamycin antibiotics which shows 18 mm diameter of inhibition zone.Struvite stones were commonest type of stones in patients with 61.5 %, followed by apatite carbonate stones with 30.7 % then mixed stones with 7.85 %.Statistical analysis shows a significant difference (P < 0.05) of the levels of blood urea, uric acid, serum creatinine and serum calcium for the patients when compared to that of control group. While no significant difference (P > 0.05) is observed or the R.B.S. level between the same groups.

علاقة الهرمونات الجنسية بصورة الدهون المصلية والاجهاد التاكسدي في عقم الرجال

Author name: ذو الفقار غانم محمد المحنه

Supervisor name: سلمان علي احمد

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Master

University: Al-Nahrain University - College Of Science - Chemistry Department

Language: English

University location: Baghdad

Author name: سنان كاظم شنان

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Doctorate

University: University Of Basrah - College Of Science - Chemistry Department

Language: English

University location: Basrah

First pages:

Abstract: Emission of gasoline and its combustion products are consideredmajor air pollutants with adverse effects on the antioxidant systems in thehuman. The effects of exposure to the vapors of motor gasoline (vehiclesfuel in Basrah), on workers were investigated. The current study included50 workers exposure to gasoline in the gasoline filling stations, ranging inage from 18 to 56 years at a mean of (30.45 ± 9.31), in addition to thecontrol group, which consisted of 50 healthy individuals (non - workingstations filling) ranging in age from 18 to 50 years at a mean of (30.27 ±10.37).The study aims to find a number of biochemical indications and theirrelationship with exposure to gasoline by estimating the activity of the enzyme (δ - aminolaevuIinate dehydratase (ALAD)) which is considered as an indicator of the extent of poisoning by heavy metals, study the activity of enzymatic antioxidant (Glutathione peroxidase (GPx), Superoxide dismutase (SOD), Glutathione reductase (GRx), Glutathione S - transferase (GST), Catalase (CAT)), non - enzymatic antioxidants glutathione reduce glutathione (GSH), study the level of Malondialdehyde (MAD), Total antioxidant capacity (TAC), trace elements (Selenium (Se), Znic (Zn), Copper (Cu), magnesium (Mg)) and heavy metals (lead (pb), mercury (Hg), Cadmium (Cd)) and the results were as follows : 1 - Highly significant decreasing (p <0.001) in workers in the levels of trace elements (Se, Cu, Zn) compared with healthy control, while significantly decreased (p <0.05) in the level of Mg with high significantly increased in the levels of heavy metals (Pb, Hg and Cd) (p <0.001). On the other hand, The results indicated that trace elements (Cu, Mg, Zn, Se) were decreased with increasing of the period of gasoline station workers while for heavy metals (Pb, Hg, Cd) increased were observed.2 - The level of MAD, the end product of lipid peroxidation, was high significantly elevated (P<0.01) contact with a significant decreasing (p <0.05) in the level of TAC in workers when compared with healthy controls. MDA increased with the increase of the period of gasoline station workers. MDA concentrations were increased significantly, indicating the amount of cellular damage. Increase the level of MDA from pollution as the period of pollution, increased might decrease TAC in workers compared with healthy control.3 - Workers group showed a highly significant decrease in the activity of enzymes (SOD, CAT, GPx, GRx, GST, ALAD) and level of GSH (p<0.001) compared with healthy controls. The results showed that increasing the duration of the work in the filling station to produceadecrease in the activity of the enzymes (SOD, CAT, GPx, GRx, GST, ALAD) and level GSH. Also, the current study include the assessment of the impact of heavy metals and MDA on the levels of trace elements (Cu, Mg, Zn, Se), (GSH, TAC) and the activity of the enzymes (SOD, CAT, GPx, GRx, GST, ALAD) through the study of the correlation coefficient (p) and the results were as follows : 1 - The level of MDA showed a highly significant negative correlation with the activity of enzymes SOD (P<0.001) and (GRx, ALAD with p<0.01), a highly significant positive correlation with levels of heavy metals (Pb, Hg, Cd), a significant negative correlation (p<0.05) with levels of (Se, Zn, Mg, GSH) and activity of enzymes (CAT, GRx) and a negative correlation with no significant (P>0.05) with level of Cu and activity of GST in workers.2 - The level of Pb shows a highly significant negative correlation with the levels of trace elements Cu, Zn, Se and activity of enzymes GRx, ALAD (P <0.01), while (p <0.001) with the level of TAC, GSH and activity of enzyme GST, GPx, SOD, a significant negative correlation with level of CAT (P <0.05) and a negatively correlated with no significant with the level of Mg in workers of gasoline stations.3 - The study illustrate also a highly significant negative correlation between Hg level and levels of (Zn, Mg, Cu, TAC) and SOD activity (P < 0.001), while there was a significant negative correlation (P < 0.05) with the level of Se and CAT, GPx, GRx, ALAD activity and a negative correlation, but not significantly with level of GSH and GST activity in serum of gasoline station workers.4 - The level of Cd showed similar behavior of other heavy metals a highly significant inverse correlation with the levels of Se, Cu, TAC and the activity of SOD (p <0.001), a significantly with the level of Zn, GSH and ALAD, CAT, GPx activity (p<0.05), while there was a negative correlation , but not significant (P > 0.05) with levels of Mg and GRx , GST activity in workers.

دراسة تركيز الهبسيدين , حالات الحديد وبعض المتغيرات الكيموحياتية في مرضى متلازمة المبيض متعدد الاكياس

Author name: دعاء نعمــــة مســـلم الزامــــــلي

Supervisor name: هناء عداي علي السلطاني

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Master

University: University of Kufa - College Of Education For Girls - Chemistry Department

Language: English

University location: Najaf

First pages:

Abstract: تعد متلازمة المبيض متعدد الاكياس ( PCOS ) واحدة من اشهر اضطرابات الغدد الصماء غير المتجانسة والاكثر تعقيدا للنساء في سن الانجاب. عادة ما تكون مرتبطة مع مقاومة الانسولين (IR) المرافق لفرط نسبة الانسولين في الدم كعملية تعويضية. الارتفاع المزمن لهرمون ( LH ) ومقاومة الانسولين هما الاكثر شيوعا لانحرافات الغدد الصماء والتي تضهر في متلازمة المبيض متعدد الاكياس.في الدراسة الحالية فان مستوى هرمون الهيبسيدين وحالات الحديد (Total Iron، Ferritin، TIBC، UIBC، %TS، TF) تم دراستها ومقارنتها مع العديد من العلامات الجسمانية من(العمر، الوزن، الطول، مؤشر كتلة الجسم، ونسبة الورك الى الخصر (WHR) والهرمونات (LH، FSH، TT، FT، SHBG، E2، Progesterone، Prolactin ) ومتغيرات مقاومة الانسولين التي تتضمن (مستوى السكر في حالة الصيامFBG، ونسبة الانسولين في حالة الصيامFIN ومقياس مقاومة الانسولين HOMA - IR) وبعض المتغيرات الكيموحياتية (الدهون الثلاثية، نسبة الكولسترول في الدم ، نسبة الكولسترول الجيد العالي الكثافة والكولسترول الضار الواطئ الكثافة) في مرضى متلازمة المبيض متعدد الاكياس ومجموعة الاصحاء. اضافة الى ذلك تم تقسيم مرضى (PCOS) الى عدة مجاميع طبقا للمعايير التالية : (وجود الشعرانية، وجود انتظام الحيض، وجود العقم الاولي والثانوي، وجود مقاومة الانسولين والحساسية للانسولين بالاعتماد على(HOMA - IR≥3 ) .تم اختيار ستون (60) مريضة مصابة بمرض متلازمة المبيض متعدد الاكياس (PCOS) تتراوح اعمارهم بين (15 - 42) سنة. تم تشخيص مرض (PCOS) من قبل طبيبة نسائية متخصصة في مركز الخصوبة في مستشفى الصدر التعليمي في النجف الاشرف خلال الفترة من (شهر ديسمبر 2014 الى شهر مايو 2015). كذلك اختيرت (30) امراة سليمة تتراوح اعمارهن بين (15 - 42) سنة. تم قياس المتغيرات الكيميائية (نسبة السكر في الدم في حالة الصيام، قياس تركيز TG، الكولسترول، HDL، Total Iron وTIBC باستخدام طرق طيفية. بينما تم قياس تراكيز كل من (نسبة الانسولين في حالة الصيام ، الهيبسيدين، Ferritin، LH، FSH، TT، SHBG وProgesterone، E2 وProlactin)باستخدام طريقة (ELISA). تم حساب مقاومة الانسولين (HOMA - IR) باستخدام المعادلة التالية (تركيز السكر في الدم في حالة الصيام × تركيز الانسولين في الدم في حالة الصيام / 22.5).في الدراسة الحالية وجد ان الزيادة في تركيز الانسولين في حالة الصيام (p=0.001) ، HOMA - IR (p=0.001) ، LH (p=0.001) ، ونسبة LH / FSH (p=0.001) وProgesterone (p=0.001) ، SHBG (p=0.001) ، TT وFT (p=0.001) ، E2 (p=0.001) ، Prolactin (p=0.001) ، الكولسترول ، HDL ، LDL والدهون الثلاثية (p=0.001 ) ، Total Iron ، TIBC ، Ferritin ، TS ٪ ، UIBC وTF (p=0.001) وهرمون الهيبسيدين (p=0.001) عندما قورنت مجموعة متلازمة المبيض متعدد الاكياس مع مجموعة الاصحاء. بينما اضهرت الدراسة الحالية عدم وجود تاثير لنقصان تركيز السكر في حالة الصيام ونسبة هرمون FSH (p>0.05) خلال المقارنة. وجد في الدراسة الحالية ان تغيير مستويات هرمون الهيبسيدين يعتمد بصورة رئيسية على حالات الحديد ومقاومة الانسولين . | Polycystic ovary syndrome (PCOS) is one of the most complex common and heterogeneous endocrine disorders of women in the reproductive age. It is frequently associated with insulin resistance (IR) accompanied by compensatory hyperinsulinaemia. Chronically elevated luteinizing hormone (LH) and insulin resistance are 2 of the most common endocrine aberrations seen in PCOS. In the present study, the level of serum hepcidin and Iron status(total iron, ferritin, TIBC, UIBC, TS%, TS.c) studied and compared with many descriptive (age, weight, height, BMI, hip/waist ratio (WHR) , hormonal (LH, FSH, total testosterone, Free testosterone, SHBG, E2, progesterone, prolactin and insulin resistance parameters including (FBG, fasting insulin, homeostasis model assessment of insulin resistance HOMA - IR) and some biochemical parameters (TG, Cholesterol, HDL, LDL) in PCOS and control groups. Furthermore, PCOS patients are sub grouped according to the following criteria : presence of hirsutism, presence of regularity of menstruation, presence of primary and secondary infertility, presence of insulin resistant and insulin sensitive state according to HOMA - IR≥3. Sixty (60) polycystic ovary syndrome patients (PCOS) of ages range between (15 to 42) year were recruited. PCOS was diagnosed in patient by the gynecologists of the Fertility Center in AL - Sadder Teaching Hospital in Najaf during the period from (December 2014 to may 2015), 30 healthy women of ages range between (15 to 42) year were included as a control group. Fasting blood glucose, TG, cholesterol, HDL, total Iron and TIBC concentrations were measured by spectrophotometric methods. Fasting insulin, hepcidin, ferritin, LH, FSH, total and free testosterone, sex hormone binding globulin, progesterone, E2 and prolactin levels were measured by enzyme linked immune - sorbent assay methods. Insulin resistance was estimated by( HOMA - IR) using the equation (Fasting blood glucose × fasting insulin/22.5) . In the present study Significant increase of the concentrations of fasting insulin (p=0.001) , HOMA - IR (p=0.001) , LH (p=0.001) , LH/FSH ratio (p=0.001) , progesterone (p=0.001) , SHBG (p=0.001) , total testosterone (TT) and free testosterone (FT) (p=0.001), E2 (p=0.001) , prolactin (p=0.001), cholesterol, HDL, LDL and triglycerides(p=0.001) , total Iron, TIBC, ferritin, TS%, UIBC and TF (g/L) (p=0.001) and hepcidin hormone (p=0.001) were obtained in the group of polycystic ovary syndrome patients group when compared with those of the control group. Fasting blood glucose and FSH showed no significance (p>0.05) were noticed during a comparable evaluation. In the present study Changes of Hepcidin levels are dependent directly on Iron status and insulin resistance

استخلاص نقطة الغيمة بالازدواج مع طريقة طيفية لاستخلاص وتقدير النيكل (??) والكوبلت (??) والبزموث (?I?) في نماذج مختلفة باستعمال مشتقي ازو مناسبين == Cloud point extraction coupled with spectroscopic method for separation, extraction and determination of Nickel( ?? ) , Cobalt( ?? ) and Bismuth ( I?? ) in different samples by use of suitable Azo - derivatives

Author name: احمد صادق عبد هاشم الحمادي

Supervisor name: شوكت كاظم جواد

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Master

University: University of Kufa - College Of Education For Girls - Chemistry Department

Language: Arabic

University location: Najaf

First pages:

Abstract: Cloud point extraction methodology was used for separation, preconcentration and extraction cations for Bismuth(III) , Nickel(II) , Cobalt(II) as ion association complexes by two new laboratory prepared azo derivatives as complexing agents which are MIBSHA and AADAD as well as these new organic complexing agents studied spectrophotometricaly by UV - Vis spectroscopy and IR specrtrophotometry to confirm their structures and for the separation, preconcentration and extraction of the elements in this study which was performed as follows : 1 - Bismuth(III) Studies : At the beginning an experiment was conducted to determine the wave length of ion association complex of bismuth ion Bi(III) with new laboratory prepared complexing agent MIBSHA by UV - Vis, spectroscopy and the spectrum showed λmax=550nm. Under optimum conditions of extraction Bi(III), the study showed that pHex=9 was more stable for extraction and gave higher efficiency because at this acidic function, there exists the best coordination bounding between Bi(III) and MIBSHA in presence of 40 μg of Bi(III) as optimum concentration giving favorites thermodynamic equilibrium for complex formation and this method of extraction depends on using Non - ionic surfactant 1% Tritonx - 100. And experimental study showed that 0.5ml of this surfactant suitable for forming Cloud point layer (CPL) with higher density and smaller volume to give the best extraction efficiency for Bi(III) with high absorbance and distribution ratio (D). The extraction efficiency also increased with increasing MIBSHA concentration as linear relation because any increasing in MIBSHA concentration is mean increasing in ion pair complex concentration and suitability so increasing in the thermodynamic equilibrium partition to the CPL. And this reflects the sensitivity of new complexing agent MIBSHA in coordination binding and ion pair complex formation in addition to extraction CPE methodology which depends on heating. The experimental study shows that the optimum temperature of heating was (90°C) that gives higher extraction efficiency because it allow to reached the best thermodynamic equilibrium in Cloud point layer formation with good dehydration, as well thermodynamic data was ΔHex=0.145KJ mol - 1, ΔGex= - 73.786KJmol - 1and ΔSex=203.669Jmol - 1K - 1 whereas the low value of enthalpy for Demonstrate extraction to approach another ion of ion pair association complex, also the extraction method is entropic region, and the experimental study about the effect heating time was 20min was the optimum heating time which gives the higher extraction efficiency with high absorbance and distribution ratio D, as this time of heating helps to reach the optimum thermodynamic equilibrium of Cloud point layer formation CPLwith higher dehydration from other hand time of heating indicate the quantity of heat which162is help to dominate on the moving of micelles inside formation cloud point layer CPL with good properties of extraction. Stoichiometric study by using four spectrophotometric methods and the study revealed that the more probable structure of ion pair complex of Bismuth ion Bi(III) extracted was [1 : 1]+ anion [Bi(MIBSHA)]2+;2No3 - experimented study about synergism effect by using Tri butyl phosphate (TBP) and methyl isobutyl ketone (MIBK) show that the extraction efficiency for bismuth ion Bi(III) increased with the presence of TBP or MIBK in aqueous solution by the effect of TBP and MIBK participation in the complex formation of Bi(III) also the experiments showed that there is one molecule of TBP or MIBK enters in the structure of ion pair complex of Bi(III) as [Bi(MIBSHA)(TBP)]2+;2NO3 - ; [Bi(MIBSHA)(MIBK)]2+;2NO3 - and TBP or MIBK enter in the complex instead of water molecule in the hydration shell of Bi(III) and increase hydrophobicity of ion pair complex and increased rate of partitioning to the CPL. As well as this study involved experiments about interferences effects as well as electrolytes effect and these experiments illustrated interferences effect to decline extraction efficiency because these ions form ion pair complex with complexing agent MIBSHA , that means a decrease in complexing agent MIBSHA concentration to form ion pair complex with Bi(III) so that electrolytes affect by increasing extraction efficiency of Bi(III) by effect of increasing Dehydration and destroyed hydration shell of bismuth ion to increase the chances of binding with MIBSHA for more stable ion pair complex. Later, this method was used coupled with suitable spectrophotometric application in the separation and determination of bismuth (III) in different samples.2 - Studies about Nickel (II)For nickel ion Ni(II) extraction from aqueous solution as ion pair complex used new laboratory prepared complexing agent AADAD , the spectrophotometric study for ion pair complex on Ni(II extracted into CPL showed maximum absorbance peak at wave length λmax=585nm and through the experimental study to limit optimum conditions and effective parameters. The study demonstrated that the optimum pHex was (9) which gives the highest extraction efficiency for nickel ion Ni(II by use complexing agent AADAD, whereas at this pHex favorable binding was given to form ion pair complex of nickel ion Ni(II with high concentration and stability as well as the method CPE for extraction Ni(II needed 0.5ml of non - ionic surfactant 1% TritonX - 100 which is affected to reach favorable thermodynamic equilibrium for aggregation of micelles in the state CMC for CPL formation with good163properties for the ion pair complex extraction of Ni(II) as well this extraction efficiency appeared with the presence of 50μg Ni(II) in aqueous solution to reach the best thermodynamic equilibrium for ion pair complex formation. Increasing AADAD concentration showed the existence of linear relation with linear increase in absorbance and distribution ratio and linear increasing in concentration stability and partitioning to CPL ion pair complex of Ni(II whereas CPE methodology depends on the temperature of heating, the experiment showed that 80°C was the optimum temperature that gives the higher extraction efficiency of Ni(II with thermodynamic data ΔHex= 0.1126KJmol - 1 , ΔGex= - 58.19KJmol - 1 and ΔSex=165.16Jmol - 1K - 1 . These values prove that the ions of ion pair complex for Ni(II) extracted into CPL approached one another with a high gegree and the method of extraction was entropic in region. The experimental study about the effect of heating time showed that 15min was the best time of heating that allows to reach a higher extraction efficiency of CPE method. Time of heating indicates the kinetic side of extraction method as heating for 15min at 80°C gave the quantity of heating that hels to reach the favorable thermodynamic equilibrium for CPL formation stoichiometry showing the more probable structure of ion pair complex extracted for Ni(II) which was [Ni(AADAD)]+;No3 - but synergism effect study showed that by using TBP and MIBK showed increasing extraction efficiency of Ni(II) with the presence of TBP or MIBK in aqueous solution because substitution water in coordination shell of Ni(II) in ion pair complex and effect to increase hydrophobicity, stability and partitioning to CPL. Also, the study revealed that adding one molecule of TBP or MIBK in the structure of ion pair complex of Ni(II) such as [Ni(AADAD)(TBP)]+;NO3 - , [Ni(AADAD)(MIBK)]+;NO3 - . So the studies tackled the effect of interferences as cations and electrolytes on extraction efficiency, later on, we used this method in the extraction and spectrophotometric determination of Nickel (II) in different samples.3 - Studies about Cobalt (II)The experimental studies concerning the separation, preconcentration and extraction of cobalt(II) as ion association complex by using the same complexing agent used with nickel (II) AADAD according to Cloud point extraction methodology. The UV - Vis spectrophotometric studies about the ion association complex extracted showed that the maximum absorbance of the complex of wave length λmax=530nm. As well as the extraction method according to CPE method showed (pH=8) was the optimum acidic function for extraction giving the highest absorbance and distribution value D because this pHex the best164coordination bonding between cobalt ion Co(II) and complexing agent AADAD so that high partitioning of stable complex to cloud point layer CPL. Moreover, the experiments showed that 0.5ml of Non - ionic surfactant, very stable for giving higher extraction efficiency because this volume of surfactant helps to reach the favorable thermodynamic equilibrium of aggregation of micelles to form CPL with high density and small volume and the micelles in case one approaches the other with the best dehydration to be in high hydrophobicity with good partitioning of complex to CPL. And in this case giving higher extraction efficiency, also, this extraction efficiency obtained when 60μg of cobalt ion Co(II was in the aqueous solution to give the best thermodynamic equilibrium to formation high stability ion pair complex extracted into CPL quantitively effect of temperature study showed that 80°C was the best temperature of extraction according to CPE method because in this temperature the best CPL was formed by aggregation and using dehydration at CMC state happen good extraction as well as thermodynamic data for extraction Co(II) was ΔHex=0.1014KJmol - 1, ΔGex= - 60.658KJmol - 1, ΔSex=171.86Jmol - 1K - 1. These data showed from the small positive value of enthalpy of extraction , the ions of ion pair complex extraction when one approach another to increase stability of this complex as well the large positive value of entropy of extraction demonstrates the dependence of extraction according to CPE and entropy. Then this procedure being entropic in region. Time of heating as the experiment study showed is 15min and was stable to give higher extraction efficiency. Because heating the solution at fixed temperature and time mean the quantity of heating help kinetically to reach thermodynamic and kinetic equilibrium for formation CPL. Then giving higher extraction efficiency. Stoichiometry study revealed that the more probable structure of ion pair complex extracted was [Co(AADAD)]+;NO3 - . Synergism study show presence TBP or MIBK in aqueous solution effect to increase extraction efficiency of Co(II) according to CPE method. Because complexing agent AADAD was not able to saturated the coordination shell of Co(II) ion in complex formation then the empty coordination position and occupy with water molecule and decline extraction efficiency , but TBP or MIBK able to coordinate instead of water in coordination shell and increase extraction efficiency, as well the study showed one molecule of TBP or MIBK able to enter in complex formed such as [Co(AADAD)(TBP)]+;NO3 - or [Co(AADAD)(MIBK)]+;NO3 - . So that the studies involved the effect of interferences as cations and electrolytes on extraction efficiency , later we used this method for the extraction and spectrophotometric determination of cobalt (II) in different samples.

تقدير البروتينات الدهنية والاجهاد التاكسدي في مصل المصابين بمرض حب الشباب == Evaluation of Serum Lipid Profile and Malondialdehyde in Patients with Acne

Author name: رنا عبد العالي الرفيعي

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Master

University: University of Kufa - College Of Education For Girls - Chemistry Department

Language: English

University location: Najaf

First pages:

Abstract: The present study was conducted to verify the relevance of lipids and lipoproteins levels and their byproduct of oxidation, malondialdehyde (MDA) in acne patients. The design of the project included one hundred acne patients (47% males and 53% females)and 40 apparently healthy individuals (control group). Total serum cholesterol (TC), high density lipoprotein cholesterol (HDL - C), triglycerides (TG), low density lipoprotein (LDL - C), very low density lipoprotein (VLDL - C) and MDA levels were measured in acne patients and the control group.The results indicated significant (P≤0.05) elevations for TC, TG, LDL - C, VLDL - C and MDA levels in acne patients with respect to those of the control group. Sex differences were obtained as rises forTC, LDL - C and MDA (P≤0.05) in males when compared with thoseof the females. The linear regression analysis exhibited significantpositive correlation for TC (r = 0.68, P≤0.05) and LDL - C (r = 0.48, P≤0.05) with ages of the control group but not in the acne patients.Smoker acne patients demonstrated significant elevations for TC, TG, VLDL - C, LDL - C and MDA (P≤0.05) in comparison with those of the control group, while nonsmokers acne patients showed significant elevations for TC and LDL - C (P≤0.05) during a comparable evaluation. Married acne patients indicated significant increases for TC, TG, VLDL - C, LDL - C and MDA (P≤0.05) when compared with those of the control group, while non married acne patients illustrated significant elevations for TG, VLDL - C, LDL - C and MDA (P≤0.05). The influence of treatment was studied, the data stated significant elevation for TG and LDL (P≤0.05), in treated acne patients when compared with those of the control group, while untreated acne patients showed significant increases for TC, TG, VLDL - C, LDL - C and MDA (P≤0.05) in comparison with those of the control group.These results suggested that oxidative stress is involved in the pathogenesis of acne. We believed that this study is the first to deal with changes of serum lipid profile and oxidative stress in acne patients.

فصل انزيم اللايبوكسيجينيز من مصل الرجال الاصحاء ومعاملة خارجية للانزيم في مرضى الربو == Separation of Lipoxygenase from Sera of Healthy men and in Vitro Treatment of Enzyme in Asthmatic Patients

Author name: احمد جياد عباس البرقعاوي

Supervisor name: عودة مزعل ياسر الزاملي

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Doctorate

University: University of Babylon - College Of Science

Language: English

University location: Babylon

First pages:

Abstract: This study is concerned with asthma. Sixty seven asthmatic male patients were examined and tested , their age ranged from 10 - 65 years and they previously diagnosed as asthmatic patient under the physician supervision in AL - Hilla center of Allergy and Asthma . They have the disease for more than five years. Forty patients were examined during the period between July2015 to October 2015, and twenty seven patients were examined during period between June to August 2016, also the control group consisted of sixty seven healthy males ranged from 10 - 65 years.In this study asthmatic patients were divided into three groups according to medication , the first group consisted of (17) asthmatic patients taking antihistamines such as loratadine , citrizine and allermine , while the second group included (24) asthmatic patients taking montelukast , and the third group included (26) asthmatic patients who were on different drugs other than antihistamines and montelukast such as steroids and beta agonists .Five milliliters of blood were obtained from each patient, serum collected absolutely free from hemolysis stored at - 20 °C until uses for measurement of the concentrations of Glutathione, Malondialdehyde, Catalase, Histamine, Serotonin, Lipoxygenase - 5, Leukotriene A4 Hydrolase, total Immunoglobulin E and specific immunoglobulin E.The results revealed that the mean of IgE level (measured by ELISA system)in serum of asthmatic group was (368.16 ±1.772 ng/ml) versus the control group (138.9±2.515 ng/ml) with a significant difference,)P < 0.001(. Asthmatic patients and patients treated with pharmacological drugs such as montelukast and antihistamines showed high sensitivity to some food, animals, plant, palm pollen, fungal and mite allergen and they showed different values between patients and control. The study showed that there is no association between the increased sensitization to food, animal, plant and fungal allergens with age of patients.The present study showed the relationship of count blood cells (WBC and RBC) in studied groups. In all studied groups, WBC values higher than control group while the RBC values showed little differences among all studied groups.According to levels of some variables (histamine, serotonin, LOX - 5 and LTA4H) in asthmatic patients, the results showed a higher mean value of serotonin in asthmatic patients (0.023± 0.004 ng /ml) compared with control group (0.002± 0.001 ng/ml, p˂0.001) In addition the value of histamine was observed high in asthmatic patients (8.511± 0.410 ng /ml)while the control group was (2.565 ± 0.140 ng/ ml) with a significant difference of the mean value of histamine between asthmatic patients and control group (p˂0.001). LOX - 5 level in serum of asthmatic patients was higher (11.080 ± 0. 190 ng/ml) than in control group (9.821 ± 0.203 ng/ml, p˂0.001.The data revealed significant high mean value of LTA4H in asthmatic patients (24.148 ± 0 .450 ng/ml) compared with control group (18.912 ± 0.459 ng/ml, p˂0.001).The result showed that histamine level was increased in the group (>40 years) with a mean value (9.67 ± 4.25 ng /ml ) . While LOX - 5 showed high mean value (11.532 ± 1.274) in the group 20 - 40 years . Also LTA4H enzyme showed high mean value (25.54 ± 3.504 ng/ml)in the group 20 - 40 years , while serotonin appeared with high mean value (0.027± 0.015 ng/ml) in the group (< 20 years) with a significant difference between levels of these variables in each age group (p ˂ 0.001)The mean concentration of an antioxidant, Glutathione(GSH), and Malondialdehyde (MDA) in serum of asthmatic patient and control groups. The mean concentration of MDA increased in asthmatic patients (11.317 ± 1.096 μmol/l) more than control group(8.733 ± 3.756 μmol/l)with a significant difference between these groups (p < 0.05). The mean concentration of GSH increased was in the control (5.106 ± 3.197 μmol/l) more than asthmatic patients(3.905 ± 1.638 μmol/l, p < 0.05).According to the results of the present study, the mean activity of catalase(CAT) was higher (1.555±0.982 U/l) in asthmatic patients when compared with control group(1.173 ± 0.502 U/l, p < 0.05).The current study also concerned with the extrection of lipoxygenase from serum of asthmatic patient and healthy men by taking 15 ml serum from ten asthmatic patients men and 15 ml from serum of ten healthy men. And these quantities of serum were precipitated by ammonium sulfate, The best ratio for precipitating serum and extraction of enzyme is 60%. The anion exchange chromatography was used for extraction of the enzyme. The molecular weight of the extracted enzyme was determined by SDS - PAGE . In this procedure one subunit of the extracted LOXs enzyme showed a molecular weight of 35KD for one chain while a recombinant pure human LOX - 5 showed a molecular weight of 38 KD. The current study used NATIVEN technique for purification of the extracted enzyme obtained from ion exchange chromatography.The results revealed the optimum pH and temperature for LOXs activity and they were 8 and 40°C, respectively.The velocity constant and maximum velocity of the extracted Lipoxygenase enzyme was detected by using PHYWE SYSTEME GMBH SYSTEM, this system had the ability to obtain the relationship between conductivity and concentration (an increase in concentration lead to an increase in conductivity).The extracted lipoxygenase enzyme Km was 2.2mM and Vmax= 140mM/min in asthmatic patients and Km was 2.7mM and Vmax= 135mM/min in controls (by applying Michaelis - Menten equation).While the enzyme Km= 5.5mM and Vmax= 238mM/min in asthmatic patients and Km= 5 mM and Vmax= 208 mM/min in controls (by applying Lineweaver - Burk equation).The extracted LOXs from asthmatic patients and controls was treated with montelukast. The Km and Vmax appeared to have low values Km 3.6mM and Vmax 153mM/min and Km 3.7mM and Vmax 147mM/min, respectively. Also the extracted LOXs from asthmatic patients and controls was treated with molsiodomine, showed low values Km 4.4mM and Vmax 182 mM/min and Km 4.3mM and Vmax 166 mM/min, respectively.

دراسة مستويات بعض مضادات الاكسدة غير الانزيمية وعنصري الحديد والنحاس في مصل دم مرض اضطرابات الغدة الدرقية في محافظة ميسان - العراق

Author name: مهدي عودة محمد الساعدي

Supervisor name: ساهرة غريب صياح

General topic: Chemistry

Specific topic: Analytical Biochemistry

Degree: Master

University: University Of Basrah - College Of Science - Chemistry Department

Language: English

University location: Basrah

First pages:

Abstract: the current study was designed to measure some Biochemical function in serum of patients with disorder of the thyroid gland (hyperthyroidism and hypothyroidism ), which can help in the early detection and follow - up of the malfunction of the thyroid gland.Specimens collected from 124 patients with malfunction of the thyroid gland Specimens were classified accord is to the type of disease ( an overactive thyroid gland = 62 , hypothyroidism = 62) and 62 blood samples in were collected from healthy non - infected persons (control group).The study included measuring automatically the levels of thyroid hormones, (FT4, FT3) and (TSH) in the serum using a Mini - VIDAS . The levels of vitamins (E, C) and beta - carotene , and uric acid were measured , also the levels of some trace elements (iron , copper) ,were measured in the serum three above groups.The results showed the following - :  The results of the present study ,the highest percentage of patients with hypothyroidism were indicated that females and the of malfunction of the thyroid gland is occurred at the third and fourth decade of life. The impact of the genetic factor in patients with excessive secretion of the thyroid gland were higher than in patients with deficient secretion of the thyroid gland. A significant increase in the level of the hormone tri - iodothyronin (FT3) and the level of the hormone tetra - iodothyronin (FT4) and a significant decrease in the level of thyroid stimulating hormone (TSH) in patients with excessive thyroid gland secretion compared to the control group. A significant decrease in the level of hormones (FT3), (FT4) and a significant increase in the level of the hormone (TSH) in patients with hypothyroidism compared to the control group.  Generally, there was a significant decrease in the level of vitamins C,E in the sera of patients with malfunction of thyroid compared to the control group. A significant increase in the level of uric acid in patients with excessive thyroid activity and a significant decrease in patients with thyroid failure compared to the control group.  A Significant decrease in the level of beta - carotene in patients with an overactive thyroid , and a significant increase in the level of beta - carotene in patients with hypothyroidism compared to the control group. A Significant decrease in the level of iron in the sera of patients with malfunction compared to the control group. A significant increase in the level of copper in patients with excessive thyroid activity and a significant decrease in patients with thyroid failure compared to the control group.