دراسة التاثير المخفض - المضاد لفرط سكرالدم للمركبات الكيميائية الفعالة المعزولة من النبات الطبي العراقي شوك البحر == Study of Hypoglycemic Antihyperglycemic Action of Active Chemical Compounds Isolated from Iraqi Prosopis juliflora Medicinal Plant
Author name:
جمال حربي حسين السعدي
Supervisor name:
عباس دواس مطر المالكي
General topic:
Chemistry
Specific topic:
Analytical Biochemistry
Degree:
Doctorate
University:
University Of Basrah - College Of Education For Pure Sciences
Language:
English
University location:
Basrah
First pages:
25T1606 - p.pdf
Abstract:
Diabetes mellitus is a disorder in glucose sugar metabolism process becauseof different reasons.leading to a great damage in body systems , therefore medicinalplants were used successfully to treat this disease .The current study was carried outIraqi prosopis juliflora to determine and investigate its hypoglycemic action in bloodof normalglycemic and alloxan induced hypoglycemic rabbits. Many generalextracts were prepared from Iraqi prosopis juliflora pod and leaves ,they are hotaqueous ,cold aqueous ,hot alcoholic ,cold alcoholic ,oils, ethyl acetate and dichloromethane extract .Moreover , some chemical families were isolated from this plantsuch as phenols ,glycosides , flavonoids , tannins ,alkaloids and saponnins.Preliminary qualitative tests were carried out for all prepared extracts thenhypoglycemic and fasted alloxan - induced hyperglycemic rabbits to know activitythese extracts in decreasing blood glucose levels in these rabbits . the resultsindicated that all extracts showed good decreasing in glucose level. Oil extractisolated from prosopis juliflora leaves showed very high activity to decrease blood glucose ,levels in normalglycemic and hyperglycemic rabbits ,where significant decreasing (P<0.05) was found at second and forth hrs , significant decreasing (P<0.01) at sixth hr. and high significant decreasing (P<0.001) at twenty forth hrs. ,whereas leaves glycosides has a great ability to decrease glucose levels in normalglycemic rabbits where a significant decreasing (P<0.01) was found at second and forth hrs., and a high significant decreasing (P<0.001) at twenty forth hrs., but in hyperglycemic abbits , a high activity was recorded and led to a significant decreasing ( P<0.01) at second forth hrs., and high significant lowering( P<0.001 ) at twenty forth .whereas ,leaves tannins extract was noticed to be the lowest affiecieney in reducing blood glucose levels ere it led to a significant lowering ( P<0.01) at twenty forth in normalglycemic rabbits , while a significant decreasing (P<0.05) was recorded at twenty forth in hyperglycaemic rabbits. Leaves ,4 - (2’ - Hydroxyethyl)phenol, Trans - phytol , 24 - Methylencycloartan - 3 - one β - hydroxylup - 20(29) - en 4 - (β - D - Glucopyranosyloxy)benzyl alcohol and Sti masterin (.Were isolated ,separated ,purified and identified by thin layer chromatography, column chromatography, gas chromatography, IR - spectroscopy ,1H,C13 - NMR ,Gas chromatography - mass spectroscopy .Also study of hypoglycemic action of four these chemical compounds isolated from leaves and pods p.juliflora , the glycosidic compound 4 - (β - D - Glucopyranosyloxy)benzyl alcohol recorded strong activity as hyperglycemic action and led to a significant decreasing ( P<0.05) at second hrs., and significant lowering( P<0.01)at fourth ,sixth hrs. and high significant decline (P<0.001) at 24 hrs in normalglycemic rabbits ,while in hyperglycemic rabbits a significant decreasing (P<0.05) at second hrs. ,significant lowering P<0.01 at fourth and sixth hrs and significant decreasing (P<0.001) at twenty forth hrs. The active compound 4 - (2 - - Hydroxyethyl)phenol showed a high hypoglycemic action were significant decline ( P<0.05) at second hrs, a significant decreasing ( P<0.01) at sixth and a high significant decreasing (P<0.001) at twenty forth hrs. in normalglycaemic rabbits ,while in hyperglycemic rabbits , a significant decline (P<0.05) was at second , a significant decreasing (P<0.01) at fourth and sixth hrs., and a high significant decreasing ( P<0.001) was recorded at twenty forth hrs..Also active compound24 - Methylencycloartan - 3 - one showed a significant decrease (P<0.05) at second hrs. , significant decrease (P<0.01) at fourth and sixth hrs., and a high significant decreasing ( P<0.001) at twenty forth hrs hyperglycaemic rabbits, while the compound α - tocopherol recorded a significant decreasing (P<0.05) at sixth hrs. , significant decrease (P<0.01) at twenty forth hrs. in hyperglycemic rabbits. The toxicity for some extracts and active chemical compounds isolated from leaves and pods was studied where no hemolytic for red blood cells was found .
