Abstract: مرض عوز نازعة هيدروجين الكلوكوز - 6 - فوسفات يعد من الامراض المرتبطة بالاطفال المصابين باليرقان الولادي , تلف الدماغ وحتى الموت . اجريت هذه الدراسة لمعرفة ضرر الاكسدة في الاطفال المصابين باليرقان الولادي وخصوصا المصابين بمرض عوز نازعة هيدروجين الكلوكوز - 6 - فوسفات . اجريت ايضا هذه الدراسة لغرض تاكيد دور جهد الاكسدة ونظام الانزيمي المضاد للاكسدة في الاطفال المصابين باليرقان الولادي في مستشفى بابل التعليمي للنسائية والاطفال في بابل . خلال هذه الدراسة الممتدة من كانون الثاني 2008 الى الثلاثين من شهر حزيران 2008 , تم اختيار 225 طفلا مصابا بيرقان ولادي (145 ذكور و80 اناث) غير معروف السبب و50 طفلا حديثي الولادة طبيعيين (35 ذكور و15 اناث) . الاطفال المصابين باليرقان الولادي تم تقسيمهم الى اطفال مصابين بنقص انزيم عوز نازعة الهيدروجين الكلوكوز - 6 - فوسفات (المجموعة الاولى) واطفال مصابين بيرقان ولادي لكن ليس لديهم نقص في الانزيم اعلاه (المجموعة الثانية) وتم فحصهم بطريقة الاختزالية وطريقة الفقاعة المتفلورة لمعرفة عدد المصابين بنقص هذا الانزيم . لقد تبين من النتائج المحصلة في هذا البحث ان الاطفال المصابين بنقص الانزيم اعلاه هم 20 طفل (8.9 %) , 15 (75%) منهم كانوا ذكورا و5(25%) كانوا اناثا ونسبة الذكورالى الاناث هي 3 : 1 . كل الاطفال المصابين باليرقان الولادي كانوا بحاجة للعلاج الضوئي اما 14 (70%) من الاطفال المصابين بنقص الانزيم احتاجوا الى عملية تبديل كامل للدم . 2(10%) من الاطفال المصابين بنقص الانزيم كانت لديهم علامات تلف بالدماغ عند وصولهم للمستشفى وقد توفوا قبل اجراء عملية تبديل دم لهم . لا يوجد تاريخ ايجابي لتناول الادوية المحفزة لهذا المرض او تناول الباقلاء . تم دراسة عوامل دموية في الاطفال حديثي الولادة في كل المجاميع وتضمنت Hb and PCV , reticulocyte Count , WBC count . لقد تبين من هذه الدراسة وجود زيادة معنوية في reticulocyte and WBC count . لقد تبين ايضا من هذه الدراسة وجود نقصان معنوي في وزن الاطفال المصابين باليرقان الولادي وخصوصا الذين يعانون من نقص الانزيم اعلاه مقارنة مع الاطفال الطبيعيين (P value < 0.05) . ان نقص الانزيم يمكن ان يؤدي الى زيادة خطر اليرقان الولادي . هذه الدراسة دلت على وجود زيادة معنوية في مستوى مالون داي الديهايد (MDA) في الاطفال المصابين باليرقان الولادي والاطفال المصابين بنقص الانزيم (P value < 0.05) بينما نقصان معنوي في مستوى كلوتاثايون (GSH) وفعالية انزيم الكيتيليز (CAT) عندما قورنت مع الاطفال الطبيعيين (P value < 0.05) . وجد ايضا علاقة موجبة بين TSB وMDA وكذلك بين GSH وHb بينما هناك علاقة سالبة بين TSB مع GSH CAT , وHb وكذلك بين MD وكلا من GSHو CAT . | Glucose - 6 - phosphate dehydrogenase deficiency is the most common enzymopathy associated with neonatal hyperbilirubinemia , kernicterus and even death . This study was conducted to know oxidative damage in neonates with neonatal hyperbilirubinemia and G6PD deficiency . Also this study was conducted to detect the role of oxidative stress and antioxidant enzymatic system in neonatal hemolytic jaundice of Babylon Maternity and Children Teaching Hospital . A prospective study was conducted during the period from 1st of January 2008 to the 30th of June 2008 in Babylon hospital of Maternity and Pediatric . Over the study period , a total of 225 full - term newborn infants with neonatal hyperbilirubinemia (145 males and 80 females) and 50 healthy neonates taken as a control group (35 males and 15 females) were studied . Newborns with hyperbilirubinemia were subdivided into G6PD deficiency (I group) and normal G6PD (II group) , a reduction method test and fluorescent spot test were used to assay the activity of G6PD . In this study , 20 (8.9%) of neonates with hyperbilirubinemia had G6PD deficiency . Fifteen (75%) of the G6PD deficient patients were males and 5 (25%) were females with male : female ratio 3 : 1 . In both normal and G6PD deficient (II and I) , reticulocyte counts and WBC were increased while PCV and Hb were decreased when compared with control group . There was a significant decrease in weight in normal and G6PD deficient groups when compared with control group . G6PD deficiency was led to increase risk of neonatal hyperbilirubinemia with significant number of them which required exchange blood transfusion 14 (70%) . All neonates with hyperbilirubinemia have received phototherapy . Two neonates (10%) with G6PD deficiency were presented with kernicterus signs and both of them died . The present study indicates significant increase in malondialdehyde (MDA) level of neonates with hyperbilirubinemia and G6PD deficiency when compared with the control group . A significant decrease in serum catalase (CAT) activity and glutathione (GSH) in neonates with hyperbilirubinemia and G6PD deficiency was found . There was positive correlation between TSB and MDA , GSH and Hb . There was a negative correlation between TSB with GSH , CAT and Hb and between MDA with GSH and CAT .

دراسة فعالية نبات عين البزون كعلاج طبيعي لمرض سرطان الدم المستحث في ذكور الفئران == Study the Activity of Catharanthus Roseus as Natural Treatment for Induced Leukemia in Male Mice

Author name: محمد عبود عياش

Supervisor name: مفيد جليل عوض | نسرين جلال محمد

General topic: Medicine

Specific topic: Clinical Biochemistry

Degree: Master

University: University of Babylon - Faculty Of Medicine

Language: English

University location: Babylon

First pages:

Abstract: Leukemia is cancer of blood - forming tissues, begin in the bone marrow characterized by highly elevated of abnormal proliferation and circulation of immature clonal hematopoietic cells. It can affect all ages around the world which in turn considered as one of public health problem.Plants considered as an important source for medical drugs manufacturing due to the presence of certain compounds with medicinal biological activity, therefore adopted in the preparation of a lot of medicines and medical drugs because of its' speed therapeutic influence and low of negative side effects which caused by chemically manufactured medications.The present study aimed to evaluate the activity and efficiency of vincristine and other important constituent in Catharanthus Roseus plant in treatment of leukemia through enhance and direct impact on some of the body's vital biological activities by using fresh crude plant as herbal medicine. This study is Cohort study design. It is monitoring, detection, and investigation study that included some of groups that completely under control; this study used in medicine, nursing, psychology and social sciences in addition to the Ecology. This study included (60) Albino male mice divided into four groups (15) mice for each. Group (A) was the control group, group (B) was mice feed orally with of C. Roseus plant, group (C) was leukemia induced mice by subcutaneous injection of (300mg/kg B.W benzene), and group (D) was Induced leukemia mice treated by oral feeding with C. Roseus plant. The experimental work was carried out in Clinical Biochemistry Department / College of Medicine / University of Babylon during the period from (October 2015 to August 2016).High - Performance Liquid Chromatography (HPLC) technique was used for measurement of Vincristine drug (VCR) in plant and vitamin D levels in mices' serum, Enzyme Linked Immunosorbent Assay (ELISA) had was used for the measurement of serum ferritin, UV - Vis Spectrophotometer was used for determination of GSH - Px and GST concentrations, while Graphite Furnace Atomic Absorption Spectrophotometer technique (GFAAS) was used for measurement of trace elements concentration of (Fe, Cu, Zn, Se). There were hematological and histopathology studies carried out by using special required equipment to make sure that leukemia disease was indeed occur.Hematological study included measurement of Hb levels and WBC count, while histopathology study included bone marrow, liver, kidney, spleen smear. Microscopic investigation for Chromosomal Aberration and Micronuclei were also had been done.Results showed that C. Roseus plant contain the concentration of vincristine drug up to 148μg/gm. There was highly significant increase of serum ferritin levels, GST activity, Iron and Copper concentration, and WBCs in leukemic group (C) (P<0.001) when compared with control group (A), while there were highly significant decrease of these parameters in group (B) as compared with the control (A) and (C) groups.Results also showed a highly significant decrease of GSH - Px activity, Vit.D levels, Zinc and Selenium concentration, and Hemoglobin levels in group (C) (P<0.001) when compared with the control group, while there were highly significant increase of these parameters in group (B) as compared with control and (C) groups. The present study found a positive correlation between Vit.D and GSH - Px activity, Ferritin and GST activity, while this study found a negative correlation between Ferritin and GSH - Px activity, serum Iron and Selenium concentration.The present study found positive effect of C.Roseus on abnormal parameters and support the biological system which was clear in group (D) which demonstrated that there were significant inhibitions to decrease or increase of these parameters than those in healthy control.

تقيم معارف العناية الذاتية للمرضى المصابين بداء السكري النوع الثاني في مركز السكري في محافظة بابل == Assessment Of Self - Care Knowledge Among Type II Diabetes Mellitus Patients At Diabetic Center In Babylon Governorate

Author name: سالم كريم هجول الجبوري

Supervisor name: سلمى كاظم جهاد

General topic: Medicine

Specific topic: Nursing

Degree: Master

University: University of Babylon - Faculty Of Nursing

Language: English

University location: Babylon

First pages:

Abstract: اجريت دراسة وصفية (عرضيه مقطعيه) للفترة من 20/10/2015 ولغاية 25/6/2016 من اجل تقييم معارف الرعاية الذاتية لمرضى السكري النوع الثاني في مركز السكري في محافظة بابل. وقد اختيرت عينة هادفة من (120) مريضا باستخدام تقنية اخذ العينات غير الاحتمالية، التي تشمل ا | A descriptive design cross - sectional study has conducted throughout the period of October 20th 2015 to June 25th 2016 in order to assess the self - care knowledge of patients with type II diabetics mellitus at diabetic center in Babylon Governorate. A Purposive sample of (120) subjects were selected throughout the use of non - probability sampling approach, that include patients who were diagnosed with type II diabetes mellitus visiting diabetic center in Merjan medical city/ Babylon Governorate. A questionnaire was constructed to achieve the objectives of the present study, comprised of four domains including (50) items. Data was collected from patients attending for or treatment diabetic center in Hilla city through the use of interview technique, during the duty shift for the purpose of receiving health care, each patients' individually interviewed, helped by the researcher and taking a corresponded period of between ( 20 - 25) minutes. The questionnaire was clear and adequate for the measurement of the phenomenon except some adjustments are made on few items according to the experts' suggestions.Reliability of the questionnaire was determined through a pilot study and the validity was achieved through a panel of (16) experts. Data were analyzed through use of the Statistical Package of Social Sciences (SPSS, Version 20). Reliability was concerned with the consistency and dependability of the research instrument. Determination of internal consistency, reliability of the questionnaire is based on computation of Alpha Cronbach's Correlation Coefficient.The findings of the study indicated that Type II diabetes mellitus age group have old age. Sample less opportunity to read and write.Results of the diabetes mellitus patients demonstrated that sample acceptable level of socio - economic status. Regarding residency it was shown that most Diabetes mellitus type II patients existed in urban areas. study sample was found to score body mass index of overweight feature. Fortunately participants showed a good knowledge about the diabetes mellitus and its complications. In regard to knowledge of sample about self - care it was demonstrated that their achievements were fine. Self - care in diabetes has been defined as an evolutionary process of development of knowledge or awareness by learning to survive with the complex nature of the diabetes in a social context. The recent study summarized that some dietary behaviors need more attention from the patient to regulate and balance the condition, It is interesting to find out that sample study had reached good scores in regard to different issues of self - care except with some exercises and activities related to foot care. As well as current study concluded that Patients' age, levels of education, family history of diabetes and marital status affect their knowledge level as overall. The study recommended that. Patients with chronic diseases like the current one need to be educated in regard to some misconceptions such as treatment of the illness, Although majority of patients know the signs and symptoms of disease, health care facility can help in emphasizing the lack of information in some areas of the subject, Some self - care results show deficits concerning the diet, appropriate strategies such as give priorities to those very vital factors in controlling blood sugar when teaching patients can be used to correct the patients dietary habits. Educational materials or programs designed to assist Patients in practicing some activities to reduce burden of the condition.

العوامل المؤثرة على نوعية حياة المرضى المصابين بالجلطة الدماغية في مركز الفرات الاوسط للعلوم العصبية في مدينة النجف الاشرف == Factors Affecting Quality Of Life For Patients With Cerebrovascular Accident In Middle Euphrates Neuroscience Center In Al - Najaf Al - Ashraf City

Author name: احمد صالح رضا القاضي

Supervisor name: سحر ادهم علي

General topic: Medicine

Specific topic: Nursing

Degree: Master

University: University of Babylon - Faculty Of Nursing

Language: English

University location: Babylon

First pages:

Abstract: خلفية الدراسة : تعتبر الجلطة الدماغية سبب رئيسي مؤثر يقلل مستوى نوعية حياة المرضى. قياس نوعية الحياة للاشخاص الذين يعانون من امراض مزمنة يعطي رؤية واضحة لتحديد اثره على مقدمي الرعاية الصحية عندما تكون النتيجة غير ممكنه. العوامل المؤثرة على نوعية الحياة لم | Background : Cerebrovascular accident is a main leading cause affectedly decrease the level of quality of life of the patients. The measurement of quality of life for population with chronic diseases gives a clear view to determine the impact on health care providers when prognosis is not possible. Factors affecting the quality of life may be vary between different age categorize such as CVA young and old CVA patients, different factors have affected the quality of life, related to the following aspects : motor impairment, physical dysfunction or dependency in activity of daily living, the presence of depression ,cognitive impairment, and speech disturbances.The main aim of the study : To identify the factors affecting the quality of life for patients suffering from cerebrovascular accident.Design of the study : Descriptive cross - sectional study design was selected to achieve the objectives of this study the factors affecting quality of life for patients diagnosed with cerebrovascular accident in Middle Euphrates Neuroscience center in Al - Najaf Al - Ashraf City, for the period between 27 October 2015 and 30 August 2016.Setting : A unique center was selected to conduct the study, the outpatient department in Middle Euphrates Neuroscience center in Al Najaf Al Ashraf City are used to collect the data.Sample of the study : Non probability ( purposive sample ) from patients with CVA (N=80) were selected to find out the factors affecting quality of life for patients with cerebral vascular accident. Data was collected by using an assessment questionnaire which was adopted and developed by the researcher to assess the factors affecting the quality of life for patients with cerebrovascular accident. It is divided of three parts : demographical data as the first part, clinical information is the second part, while the specific CVA quality of life questionnaire which extended to 48 items distributed upon 12 main domains as (energy and activity, family relationship, speaking, movement, mood, personality, self - care, social role, thinking, in addition upper extremities functions, vision, and work / productive ) is the third part. Validity of the questionnaire obtain by (19) experts, who work in different fields to obtain the reability of the questionnaire alpha cronbach was used as a statistical method.The majority of the study sample were between (59 - 69) of age. The highest percentage of the study sample were males and were married. On the other hand the highest percentage of the sample were illiterate , housewives and urban area residents. The scores of the quality of life of the majority of the study sample were high effected regarding the following domains : (energy and activity), plus movement, moderate effect presented related to the family relationship, speaking, mood, self - management, social role, thinking, function of the upper extremities, vision, and job / productive, while low effect score recorded for personality.Conclusion : The overall quality of life level of the CVA patient who participate in the study was moderate. The QOL for patient with CVA were significantly effected related to their age, occupation, and educational level. Chronic diseases, type of CVA and it's duration play as highly effecting factors related to the patient's quality of life.Recommendation : Establishing structured program realizing the challenges that might face the post CVA patients because this long journey need special planned care to improve patient's quality of life by special rehabilitation unit, the unit services may be extended for the patient's family.

الانماط الجينية والبروتين الرابط للرتينول 4 في مرضى الاعتلال الكلوي السكري النوع الثاني == Gene Polymorphism And Retinol Binding Protein 4 In Type 2 Diabetic Nephropathy Patients

Author name: احمد جبار عباس

Supervisor name: مها فاضل سميسم | علاء حسين ال حيدر

General topic: Medicine

Specific topic: Clinical Biochemistry

Degree: Master

University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry

Language: English

University location: Babylon

First pages:

Abstract: داء السكري هو مرض ايضي له تاثير على ايض الكاربوهيدرات والدهون والبروتينات. ومن مضاعفات السكري اعتلال الكلية السكري، الذي يعد السبب الرئيسي للفشل الكلوي المزمن. الليبوكالينات هي عائلة من البروتينات التي تنقل الجزيئات الكارهة للماء صغيرة مثل السروئيدات | Diabetes mellitus (DM) is a metabolic disease involving carbohydrate, lipid, and protein metabolism. Diabetic nephropathy (DN) is the significant complication of diabetes, which is at the present time the major cause of chronic renal failure. The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, retinoids, and lipids. Retinol binding protein 4 (RBP 4) is a member of the lipocalin family of proteins and it has a role in insulin resistance. A meta - analysis demonstrated a significant association between the polymorphism of glucose transporter1 gene with DN. The presence of endothelial nitric oxide synthase (eNOS) polymorphisms may contribute to a decreased eNOS activity and a lowered nitric oxide level, and has been reported to be a potential factor in the development and pathogenesis of DN. The study aimed the evaluation of lipid profiles and determination of their association with retinol binding protein 4 expression in diabetic patients, evaluation of gene expression of eNOS G894T polymorphism in diabetic patients to predict its relationship with nephropathy in future, and evaluation of gene level of GluT1 polymorphism in diabetic patients to predict its relationship with nephropathy in future. This study was conducted in Hilla city, from December 2015 to April 2016. The samples were collected from Babylon Center for Diabetes and Endocrinology in Marjan Teaching Hospital in Babylon / Hilla city.This study was included 160 subjects, who were (35 - 58) years old and BMI between (25 - 29.9) which were divided into groups : Control group 80 apparently healthy persons included 40 male control group (MC) and 40 females control group (FC). Eighty patients with diabetes mellitus type 2 include (40 males group (M)) and (40 females group (F)). Blood samples (10 ml) were taken from fasting subjects. Two milliliters of blood were put in EDTA tube used for the determination of HbA1c and genetic study and 8 milliliters of blood were put in plane tube and centrifuged to separated serum to measure (glucose, insulin, retinol binding protein 4 and lipid profile ( and insulin resistance was measured by equations. The results show : 1. There is a significant increase in the mean of fasting glucose, HbA1c, fasting insulin level, insulin resistance in groups M and F as compared to the control groups, (p<0.01), but there was no significant increase between two patient groups (p ? 0.05)2. The significant increase (P <0.01) in total cholesterol (TC), TG, LDL - C and VLDL - C, while serum HDL - C concentration was found significantly decrease (P<0.01) in patient groups as compared with the control groups, and no significant increase or decrease in lipid profiles in comparison between patients groups (P ? 0.05).3. There is significant increase (P< 0.01) in RBP4 between patients and control groups, and not significant (P?0.05) difference between patient groups.4. Group M shows significant positive correlations (r = 0.514, p= 0.0012 and r = 0.536, p = 0.0034) of RBP4 concentration with serum fasting insulin concentration and HOMA - IR respectively, in group F, the results show a significantly positive correlation (r = 0.502, p = 0.001 and r = 0.474, p = 0.02) between RBP4 concentration with serum fasting insulin concentration and HOMA - IR respectively.5. A significant positive correlations was found (r = 0.486, p 0.001; r = 0.388, p 0.013; r =0.385, p=0.014) for serum RBP4 concentration with TC, TG and LDL - C concentrations respectively, and significantly negative with HDL - C (r = - 0.424, p =0.006) in group M, also positive correlation (r = 0.443, p =0.004; r = 0.467, p = 0.002; r = 0.435, p = 0.005) of RBP4 with TC, TG, LDL - C respectively and negatively with HDL - C (r = - 0.453, P = 0.003) in group F.6. A significant increase (< 0.01) in non HDL - C between patients and control groups and no significant (?0.05) difference between patient groups.7. According to the results of genotyping, XbaI polymorphism wasidentified as homologous genotype XbaI ( - / - ) were 12 (30%) in the group M, 14 (35%) in the group F, 3(7.5%) in the group MC and in the group FC 2(5%), while individuals have heterozygous genotype XbaI ( - /+) were 7 (17.5%) in the group M, 10 (25%) in the group F, 10 (25%) in group MC and in the group FC 8(20%).8. A significant association between the frequency of XbaI ( - / - ) variant in group M in compared with group MC an odd ratio = 5.14 and confidence interval at 95% level of (1.36 - 19.4), and in group F in compared with group FC an odds ratio = 13.12 and confidence interval at 95% of (2.64 - 65.07). 9. According to the results of genotyping, G894T polymorphism for eNOS gene was identified as homologous genotype TT were 7 (17.5%) in group M, 9 (22.5%) in group F, 8 (20%) in group MC and 10 (25%) in group FC, while individuals have heterozygous genotype G/T were 21 (52.5%) in group M, 20 (50%) in group F, 6 (15%) in group MC and 7 (17.5%) in group FC.10. A significant frequency of GT variant in group M is compared with group MC with odds ratio = 7.58 and confidence at 95% level of (2.43 - 23.62), and in patients of group F as compared with group FC with odds ratio = 7.27 and confidence interval at 95% of (2.4 - 22.02).In conclusion, the RBP4 is associated in causing insulin resistance and lipid abnormalities. The high levels of Non HDL - C in diabetic patient contribute in progression of diabetic nephropathy. The GLUT1 polymorphism in diabetic type 2 patients specially patients with XbaI ( - / - ) and eNOS gene polymorphism G/T allele have a role in progression to diabetic nephropathy.