مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: The present study had been carried out to elucidate the toxic effects of sodium fluoride on local rabbit males, this study involved determination of the histological Changes of some organs include (Testes, Liver and Kidney) , moreover study the changes in body weight of the animals and the percentage of organs weight and some blood parameters and biochemistry which include packed cell blood volume (PCV), Hemoglobin (Hb), Total white blood cells, Testosterone hormone, T3, T4 hormones, Glucose concentration, Cholesterol, Urea, Transaminase enzymes and Bilirubin - level in blood. Twenty local adult male rabbits were included this experiment their ages ranged between 10 - 14 months.The animals were treated with oral swallow for 12 weeks. The animals were divided into four groups : control group is treated orally with (0.9%) normal saline; First group was treated orally with 10 mg/kg of body weight sodium fluoride, second group was treated orally with 20 mg/kg of body weight sodium fluoride and third group was treated orally with 30 mg/kg of body weight sodium fluoride. The results of present study showed a significant decreased (P<0.05) in body weight of treated animals with dose of sodium fluoride 20 and 30 mg/kg of body weight, while there is no significant differences in the body weights of treated animals with sodium fluoride dosage of 10 mg/kg of body weight when to compared with control group. The percentage of organ weight to body weight showed a significant decrease in testis weights and liver weight, while there was no significant differences in kidney weight in treated group with sodium fluoride when to compared with control group. Concerning histological changes showed a reduction or freezed stages in spermatogenesis processes which timed with decrease in number of primary spermatocytes and secondary spermatocytes in addition to infiltration of lymphocytes, also found that NaF cause disorganization, denudation, and reduction in germinal epithelial cells of the seminiferous tubules and associated with absence of sperm in the lumina, also showed a decrease in radius of epididymus ducts and decrease in number of sterocilia and decrease number of mature sperms. Histological sections of kidney showed that there was a clear shrinkage of glumeruli.Histological section of kidney of animals that treated with sodium fluoride showed a mild blood vessels congestion, shrinkage in glumeruli, distributed in renal tubules and erode its epithelium with some apoptotic areas. The liver sections appeared appreciable effect with fluoride include dilation in central vein and erode to its epithelium, congestion in hepatic sinusoids with apoptosis and erode to general structure of liver with some of inflammatory cells and death in some cells which lead to erode of typical ray arrangement. Regarding blood parameters the present study showed a significant decrease (P<0.01) in percentage of (PCV) and (Hb) of the animals treated with sodium fluoride, while showed (WBCs) count significant increased compared with control group. Hormonal analysis (Testosterone, T3 and T4) showed a significant decrease, while significant increase of glucose, cholesterol, urea, transaminases enzymes for (AST, ALT) and bilirubin levels. Results obtained from this study and mentioned above may be attributed essentially to that of Naf administration results in sever lesion of male reproductive system wich may be aggravated with increase administration concentration of Naf.

دراسة وراثية خلوية وجزيئية للتاثير الوقائي للكركم في سمية العقار ميترونيدازول في الفئران البيض == Cytogenetic And Molecular Study of Protective Effect of Curcumin in Toxicity of Metronidazole in White Mice

اسم المؤلف: فائق ابراهيم علي محمود

اسم المشرف: عادل فوزي شهاب

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: دكتوراه

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: صممت الدراسة الحالية لغرض التعرف على التاثيرات السمية الوراثية والسمية الخلوية للعقار ميترونيدازول(MTZ) في الفئران البيض Balb/ C والتاثير الوقائي الذي توفره المعاملة المسبقة بالكركم(CUR) في سمية العقار ميترونيدازول. تم استخدام مؤشرات الوراثة الخلوية (النو | The current study was designed to identify the genotoxicity and cytotoxicity of Mitronidazole (MTZ) and protective effect of Curcumin (CUR) against the toxicity of MTZ in whit mice. Cytogenetic endpoints (micronuclei (MN) and mitotic chromosomes), Productive biomarkers (sperms), molecular cytogenetic biomarkers (comet assay), molecular endpoints (genomic and mitochondrial DNA concentration in liver and spleen and testis) and mutation assessment in mitochondrial DNA (mtDNA) of liver and testis by PCR and restriction pattern were used to evaluate genotoxicity, cytotoxicity of MTZ and protective effects of CUR in white Balb/Cmice. The study was conducted in the laboratories of college of education, college of science in Tikrit university and laboratories in the state company for drugs industry - Samarra - Iraq (SDI). Dosages of 30 and 50 mg/kb.b.wt. of MTZ were tested for genotoxic and cytotoxic and mutagenic effects in somatic and sperms of white mice. The dosages 4.5 and 10 mg/kgb.wt. of CUR were used to study the protective effects of CUR in genotoxicity and cytotoxicity and mutagenecity of MTZ. The current study showed that MTZ has genotoxic effect expressed by high frequency of micronuclei in polychromatic erythrocytes (PECs) which reached 15.5 ± 2.88 with the dosage 30 mg/kg.b.wt. and 21.31 ± 4.2 with the dosage 50mg/kg.b.wt. compared with 7.25 ± 1.88 in negative control. In the study of the protective effects of CUR against genotoxic and cytotoxic effects of MTZ, we observed that in mice treated with the dosage 4.5 mg/kg.b.wt of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ, There were significant p<0.05 decrease in MN frequency which reached 13.25 ± 4.19 compared with 21.31 ± 4.2 for mice treated with the dosage 50mg/kg.b.wt. of MTZ withoutCurcumin. The study showed that MTZ has cytotoxicity expressed by significant p<0.05 decrease in mitotic index (MI) which reached 0.010 in bone - marrow of the mice treated with 50 mg/kg.b.wt. compared with 0.023 in negative control. The dosage 4.5 mg/kg.b.wt. of CUR for 9 days before giving the dosage 50 mg/kg.b.wt. of MTZ resulted in returning of MI in bone - marrow 0.021 to its levels in negative control 0.023 compared with its value which reached 0.010 with the dosage 50 mg/kg.b.wt. of MTZ. The two dosages 30 and 50 mg/kg.b.wt. of MTZ caused significant p<0.05 decrease in total number of sperms which reached 2.4±2.4 and 1.5 ± 0.1 milions simultaneously compared with 6.7 ± 0.9 millions in negative control. More over there were significant p<0.05 decrease in motile sperms which reached 25.2 ± 11.0 and 14.6 ± 10.6 compared with 57.6 ± 8.0 of negative control. Significant p<0.01 decrease in the length of mid - piece and total length of sperm tail with the dosage 50 mg/kg.b.wt. of MTZ which reached 19.2 ± 6.0 and 69.0 ± 6.0 simultaneously compared with 31.6 ± 9.0 and 100.3 ± 15.0for negative control. Treating with the dosage 4.5 mg/kg.b.wt. of CUR for 9 day before the dosage 50 mg/kg.b.wt. of MTZ resulted in the return of mid - piece and total length of sperm tail which reached 29.5 ± 3.0 and 95.0 ± 5.0 close to the levels of negative control 31.06 ± 9.0 and 100.3 ± 15.0 simultaneously. Compared to 19.2 ± 6.0 for the length of mid - piece and 69.0 ± 6.0 for total length of sperm tail in mice treated with the dosage 50 mg/kg.b.wt. of MTZ without previous treatment with CUR. The study of damage levels in DNA with comet assay showed that MTZ induced significant p<0.01 levels of damage reached 45.4 ± 2.36 with the dosage 50mg/kg.b.wt. of MTZ in bone - marrow and 36.8 ± 0.58 in liver cells compared with 9.0 ± 2.12 and 5.2 ± 0.58 simultaneously in negative control. There were decrease occurred in the level of damage in DNA of bone - marrow and liver cells. However it did not return to its levels of negative control 9.0 ± 2.12 in bone - marrow and 5.2 ± 0.58 in liver cells. OTM values reached 26.4 ± 1.29 and 24.0 ± 1.14 in mice treated with the dosage 4.5 mg/kg.b.wt. of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ. However The values of OTM lower than its values in bone - marrow 45.4 ± 2.36 and 36.8 ± 1.66 in liver cells of mice treated with the dosage 50 mg/kg.b.wt. without CUR. In the molecular study, The results showed mutagenic effect of the dosage 50 mg/kg.b.wt. of MTZ in mice mtDNA expressed by novel restriction sites for Bam - HI and Hind - III in liver tissue and SauA3 in testis. The treatment of mice with the dosage 4.5mg/kg.b.wt. of CUR for 9 days before the dosage 50mg/kg.b.wt. of MTZ did not resulted in the inhibition of mutagenesis in mtDNA. In conclusion, results of this study showed that MTZ has genotoxic and cytotoxic effects in white mice and mutagenic effect in mice mtDNA. The dosage 4.5 mg/kg.b.wt.of Curcumin has protective effect against genotoxic and cytotoxic effects of MTZ. on the other hand the dosage 4.5 mg/kg.b.wt. of Curcumin has no protective effect against mutagenicity of MTZ in mice mtDNA.

دراسة عدد من المتغيرات الفسلجية والمناعية لدى النساء المصابات بالتهاب السبيل البولي في مدينة تكريت == Study Some of Physiological And Immunological Parameters in Women With Urinary Tract Infections in Tikrit City

اسم المؤلف: لبنى عبد الجبار ياسين

اسم المشرف: موسى جاسم محمد الحميش

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: ان الهدف الرئيس لهذه الدراسة هو معرفة علاقة الانترلوكينات(IL - 2, IL - 4, IL - 6) ببعض الهرمونات لدى النساء المصابات بالتهاب السبيل البولي.تضمنت هذه الدراسة 116عينة دم من النساء المراجعات للعيادات الخارجية التابعة لمستشفى تكريت التعليمي اللواتي تترواحت اع | The main objective of this study is to investigate the relationship of Interleukines (IL - 2, IL - 4, and IL - 6) with hormones in women with inflammation of the urinary tract. This study involved (116) women visiting outpatient clinic of Tikrit Teaching Hospital for the period from September 2011 until February 2012. The ages of women were 16 - 50 years, and blood samples were taken for each of these women for the purpose of determining the levels of sex hormones (FSH, LH, estrogen, progesterone, testosterone) and the levels Interleukines (IL - 6, IL - 4, IL - 2) and identify some of the variables blood parameters (WBC, ESR) and C - reactive protein Twenty samples were taken from the blood of women who are not infected and non - pregnant as a control group samples. The patients divided into three groups, which included non - pregnant women infected and pregnant women who are infected and pregnant non - infected urinary tract (UTI) also samples of the study was divided by periods of gestation into three periods included the first period (1 - 12 weeks) and the second period (12 - 24 weeks) and the third period (24 - 40 weeks) of pregnancy The results of the current study showed that there is significant increase in ESR for a pregnant women infected and pregnant women non - effected with UTI when compared to control (P ? 0.01) and highest level of ESR was in the last third of pregnancy, The total Count of white blood cells (WBC), results showed a significant difference between the three groups compared to the control group, had the highest level for (WBC) in the last third of pregnancy. As for the study of hormones when measuring the level of the hormone LH showed a significant decrease (P ? 0.05) for the three groups compared to control and the steepest decline of hormone level was in the last third of pregnancy. The hormone FSH got a significant decrease in the three groups compared with a control (P ? 0.01), lowest level was in the third trimester of pregnancy.Estrogen level showed a significant decrease (P ? 0.01) for a non pregnant women with UTI compared with a control, and show a significant increase in the level of the two sets of hormone - positive pregnant non - infected, and the highest increase was in last third of Pregnancy in both groups. The progesterone showed a significant increase in the level of the pregnant infected and pregnant non - infected (P ? 0.01) compared with a control group. While in non - pregnant, there is no significant difference. The testosterone level increased significantly (P ? 0.05) in the three groups compared to control group. the level Interleukines, Tests showed that a significant increase(p ? 0.01) in the level of interleukin - 2 (IL - 2) for a pregnant women with (UTI) compared with a control and a significant decrease in the last third of pregnancy in pregnant women which is non infected compared to the rest of the gestation periods, and for the level of interleukin - 4 The present study showed a significant increase (p ? 0.01) in the level of interleukin - 4 (IL - 4) for a non - pregnant women with (UTI) compared with a controlThe highest level reached by IL - 4 was in the last third of pregnancy in the non - infected at (p ? 0.05), interleukin - 6 has a significant decrease for the two sets of pregnant women with (UTI) and non - infected, compared with the control group and the absence of significant differences between the two sets of pregnant women infected and non - infectedRegarding level of C - reactive protein there is a significant increase (P ? 0.01) in the two groups of pregnant and non pregnant women compared with a control, and significant differences between control group and a group of pregnant non - infected.

تقييم استخدام الانترليوكينات في تشخيص تسمم الدم الجرثومي لدى الاطفال حديثي الولادة في تكريت == Evaluation Uses The Interleukines for Diagnosis of Neonatal Sepsis in Tikrit

اسم المؤلف: هبة حازم صالح

اسم المشرف: موسى جاسم محمد الحميش | عاشور رفعت سرحت

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: ان الهدف الرئيس لهذه الدراسة هواستخدام الانترلوكيناتInterleukin - 8, Interleukin - 10 (IL - 8, IL - 10) كعلامة تشخيصية للتسمم الدموي الجرثومي لدى حديثي الولادة. تضمنت الدراسة 45 عينة دم من الاطفال حديثي الولادة الراقدين في ردهة الاطفال في مستشفى تكريت الت | The main aim of this study was uses the Interleukines IL - 8 and IL - 10 as diagnosis marker for neonatal sepsis. This study involved 45 blood samples, were taken from neonates admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically diagnosed as sepsis for the period from September 2011 until March 2012. The ages of neonates were 1 - 30day, and this blood samples were taken for the purpose of determining the levels Interleukines IL - 8 and IL - 10 and identify (WBC and C - reactive protein). Seventeen samples were taken from the blood of neonates without of septicemia as a control group samples. The results of the current study showed non significant differences in total white blood cells count for patients with sepsis when compared with a control group. and showed non significant differences in total white blood cells count for patients with sepsis as gestation birth, sex and birth weight compared to the control group. Regarding level of C - reactive protein there was no significant differences between the control group and the patients with sepsis as gestation birth and sex. and showed a significant differences in C - reactive protein level for patients compared to the control group as birth weight. The Interleukine levels showed that a significant increase in the level of interleukin - 8 (IL - 8) for all patients mals and femals, preterm or full - term and who there weight < 2.5 or >2.5kg compared with a control group. this results showed that the relationship between IL - 8 and WBCs was not significant differences at (p<0.05). and for the level of interleukin - 10 the present study showed a significant increase for patients with sepsis as gestation birth, sex and birth weight compared to the control group, the relationship between IL - 10 and WBCs was not significant differences at(p<0.05). The relationship between IL - 1o, IL - 8 and WBCs was not significant differences. when comparison The results as end result for sepsis show is the absence of significant differences (p<0.05) for patients who became good and who had complications compared with the control group. At last, the study concluded that the Interleukines IL - 8 and IL - 10 levels increase in early time for Inflammation ;So its agood diagnosis marker for neonatal sepsis.

تقيم بعض العناصر النادره ومستوى المالونداي الدهايد والبروتين في الرجال العقيمين == Assessment of Some Trace Elements, (MDA) And Protein Levels In Infertile Men

اسم المؤلف: زهراء فلاح عبد العالي عنوز

اسم المشرف: علاء الدين صبحي محسن السلامي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية العلوم - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: تم اجراء هذا البحث لدراسه عينات السائل المنوي التي تم الحصول عليها من المرضى المصابين بوهن النطف وعددهم 35عينه كذلك سوي النطف عددهم 40 عينه وتم اخذ مجموعه من الاشخاص الاسوياء (مجموعه السيطره) حيث كان عددهم 20 عينه الذين راجعو مركز الخصوبه في مدينه الصدر ا | This study was performed on human semen specimens obtained from Asthenozoospermic patients (35 specimens) Normozoospermic males (40 specimens), and (20 specimens) Fertile Control group, who were attending to the laboratories of Fertility center in ALSader Hospital of AL - Najaf AL - Ashraf city during the period extended from 1 - 9 - 2013 to 30 - 1 - 2014. The aim of This present study was to estimate the levels of some Trace element (Lead, Copper, Cobalt, Chromium, , and Cademium) concentrations in Asthenozoospermia and Normozoospermia. by atomic absorption method, and comparsion with control (Fertile).Moreover, it tends to estimate the concentration of (MDA) Malondialdehyde , Total protein concentration and to know the viability sperm percent in semen specimens.Also, the relationships between mentioned components and some semen parameters. The results revealed significant increase (P <0.05) in the concentration of examined trace elements in Asthenozoospermia and Normozoospermia when comparsion with control, also a significant increase (P <0.05) in total protein concentration, also in MDA. While The results revealed significant decrease (P <0.05) in the viability sperm percent in semen specimens. Correlation study showed positive relationship between concentration of the trace elements and abnormal sperm morphology percent, also with total protein concentration, and MDA concentration. While negative relationship between concentration of trace elements and percent of sperm motility.It was concluded that the increase in the concentration of the trace elements and it influence on blance and parameter of seminal fluid, also the Correlation between trace element and Protein in semen. All of this lead to arise of cases of Asthenozoospermia and Normozoospermia infertile patients.

تاثير اضافة عناصر الكالسيوم والمغنيسيوم والحديد الى الوسط الغذائي في حياتية خنفساء الطحين الصدئية Tribolium castaneum (Herbst) Tenebrionidaci) Coleoptera :) == Effect of Calcium, Magnisium And Iron Elements With Various Nutreint Sources on Biology of Tribolium Castaneum (Herbst) Tenebrionidaci) Coleoptera : )

اسم المؤلف: حوراء ياسين احمد عبد

اسم المشرف: برهان مصطفى محمد الدليمي | عواد شعبان داود الناصري

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: تناولت الدراسة اختبار تاثير اضافة ثلاثة عناصرمعدنية الكالسيوم (Ca) المغنيسيوم (Mg) الحديد (Fe) في سطين غذائيين (الطحين والجريش) لصنفين من الحنطة (ابو غريب والعز) في حياتية خنفساء الطحين الصدئية الحمراء Tribolium castaneum (Herbst). اذ استخدمت العناصر بترا | This study includes testing the effect of addition of three elements (Ca, Mg, Fe) in flour and crushed partially debranned wheat for two kinds of wheat : Abu - ghareeb and Al - is in the life of Tribolium castaneum (Herbst).using the elements under different concentrations (0.01 - 0.03 - 0.05) g.to know their effects in Tribolium inculuding : (the number of eggs, period of incubation eggs, number of Larvers, number of pupal, number of external insect, new generation) after adding to nutreint base for the two kinds of wheat.This experiement must be in dark incubation at 35±2°C & relative humidity 75±5% during one generation the result show most important role of nutreint element in life of insect (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation). on side of period incubation eggs period generation there is not any mentioned effect and the most meaningful one is in kind of Al - Is comperad with Abu - ghareeb. It is noticed the maxi effect for Calisum element.It achieved the highest level in : (the number eggs, period incubation eggs, number of Larvae, number of pupal, number of external insect, new generation) to ratio of flour kind of Al - Is and achieved maxi number : (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation)in concentration (0.05)g.while the highest in ratio of generation agents in (Fluorine)element ratio(76.2) under concentration (0.03)g, it achieved the less ratio in the dead insect kind of Abu - ghareeb7s the highest in average number eggs, number of larvers for calisum element.while the highest value in average number of pupal, number of external insect, new generation to (Magnasium) element it is achieved the highest in number of eggs, number of Larvers, number of pupal, number of external insect, in concentration (0.05)g and the higher of average ratio new generation is (66) and the higher ratio new generation (79.3) is concentarte (0.03)g.It is noticed the relationship between nutreint element and life of insect is parallel, that is, concentrates increased, number of growing also increased except (Fluorine) element the relation is inversion. on side of crushed partially debranned wheat the effect is more meaningful in kind of Abu - ghareeb than in Al - Iz.Calicum element shows acceptable results, it appeared the higher in : (the number eggs, , number of Larvers, number of pupal, number of external insect) in Abu - ghareeb and new generation in Al - Iz. In the test of the effect of nutreint element agents in life of this insect according to the nutril base for both kinds.the result show that nutrient base of flour is more effective than the nutrient base crushed bartially debranned whaet in all steps of insect life for both kindc in flour maxi In flour maxi number in new generation about (62.5)g and the main ratio of new generation in crushed partially debranned wheat (36.3) for Abu - ghareeb kind (6.7)in flour. (61.1) crushed partially debranned wheat For Al - Is which shown the same features of the results of the effect in nutrient base without adding any elements to its life of insect

تاثير استخدام عقار الكلوميفين ستريت والقهوة العربية وفيتامين E على بعض المعايير الفسلجية والكيموحيوية والنسجية في الارانب البيض المعرضة للاجهاد التاكسدي ببيروكسيد الهيدروجين == Effect of Clomiphene Citrate, Coffea Arabica And Vitamin E Use on Some Biochemical, Physiological And Histological Parameters in Albino Rabbits Exposed To Oxidative Stress Induced By Hydrogen Peroxidase

اسم المؤلف: اسماء خالد مطني محمد

اسم المشرف: زيد محمد مبارك المهداوي

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الصفحات الاولى:

المستخلص: تم في هذه الدراسة اختبار فعالية عقار الكلوميفين ستريت Clomiphene citrate والتحري عن تاثيراته في نسيج الرحم والخصى وكذلك الهرمونات الجنسية والتكاثرية Follicular Stimulating Hormone(FSH), Luteinizing Hormone (LH) , Prolactin, Estrogen, Testosterone, Progest | This study includes the effect of clomiphene citrate drug in uterus and testes tissue and sex, reproduction hormone (Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL), estrogen, progesterone, testosterone) and in Some Biochemical and Physiological Parameters as : WBCs counts, glucose, total cholesterol (TC), triglycerides (TG), High density lipoproteins - cholesterol (HDL - C), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoproteins - cholesterol (VLDL - C), Total protein, albumin, globulin, glutathione (GSH), malondialdehyde (MDA) concentrations in males and females Albino rabbits sera which have oxidative stress induced by hydrogen peroxide (0.5%) for (20) days, compare with the control rabbits.The study involud : - comparison the effects of vitamin E effects and (100mg\kg) body weight of arabica coffea.The animals distributed and randomly divided to(8) groups (each group included 8 rabbits : 4 males+ 4 females) as follow : control group, Hydrogen peroxide (0.05) group, (2mg\kg of B.W) clomiphene citrate (c.c.) group, (2mg\kg of B.W) clomiphene citrate group, (500mg\kg of B.W) vitamin E+(10mg\kg of B.W) clomiphene citrate, (100mg\kg of B.W) Arabica coffea group, Hydrogen peroxide (0.05) +(100mg\kg of B.W) Arabica coffea group and (10mg\kg of B.W)(c.c.) +(100mg\kg of B.W) Arabica coffea group. The results revealed : - 1 - The treatment of males and females of Animal by clomiphene citrate (2 and 4 mg\kg of B.W) cased significant increase at leve (P? 0.05) in concentration of the FSH, LH, estrogen and progesterone hormone, and Glucose, Triglycerides, Globulin, LDL - C, VLDL - C and Glutathione in both male and female compare with the control group, while the same treatment caused increase the concentrations of Testosterone and Prolactin for males, whereas in females the treatment showed increase in Testosterone and Prolactin concentrations Compare with the control. Also the treatment caused decrease Albumin concentration in male and no significant (P? 0.05) changes had been showed in the female s. Respect to total protein, WBCs count there were no significant variation (P?0.05) had been recorded for both sexes compare with the control group. So the result showed Significant increase (P? 0.05) in HDL - C in male only compare with the control group. 2 - The induced oxidative stress caused significant increase at level (P?0.05) WBCs count, Glucose, Total cholesterol, triglycerides, VLDL - C, LDL - C, MDA concentrations for both sexes compare with the control group.While there were no significant variation had been recorded in Albumin and Prolactin concentrations in Females and total protein concentrations for both sexes compare with the control group in another hand there were significant increase (P<0.05) in concentrations of HDL - C, Total protein, Albumin, Globulin, Glutathione, Progesterone, Testosterone, Estrogen, FSH, and LH of male compare with the control group.3 - The treatment of Rabbits (male and female) with (100mg\kg of B.W) Arabica coffea revealed significant decrease (p ?0.05) in concentration of FSH, LH, estrogen, glucose, total cholesterol, triglycerides and VLDL - C for both sexes, and PRL, testosterone , glutathione and albumen in concentrations for male compared with the control group while there treatment. 4 - Treatment males and females albino rabbits by high dose vitamin E caused significant decrease in concentration of FSH, estrogen, total cholesterol, Triglycerides and Globulin for both sexes, compared with the control group, Whereas caused also a significant decrease in concentrations LH, PRL, testosterone in male, and caused a significant decrease in concentration HDL - C in female. Whereas caused also significant increase in concentration of the glucose, MDA, glutathione and the total number of white blood cells for about sexes, and caused also significant increase in concentration of the hormones PLR, testosterone in female only. Whereas caused also a significant decrease in concentrations of HDL - C, LDL - C and VLDL - C in male, whereas no significant variation in concentration of total protein, albumin for both sexes, and in concentration of LDL - C and VLDL - C in female compared with the control group.5 - Treatment males and females albino rabbits by high dose vitamin E, clomiphene citrate, Arabica coffea and H2O2 (0.5%) showed important histological changes in testes and uterus compared with the control group. Treatment males by clomiphene citrate caused significant increase (P<0.05) in sertoli cells, Leydig cells, spermatids and Spermatogonia in male, whereas in uterus tissue treatment caused significant increase in granular cells, hypertrophy smooth muscular and increase cytoplasm size compared with the control group.6 - Treatment by high dose vitamin E and Arabica coffea caused damage in somniferous tubules decrease in sertoli cells, Leydig cells, spermatids and spermatogonia in male, whereas in uterus tissue treatment caused effected uterus gland and loss of normal form and the incident of degeneration, necrosis in the surrounding cells and decrease in vassals blood compared with the control group.The present study showing that to drug clomiphene citrate play an important role to activation the oogenesis and spermatogenesis through stimulating gonadotrophic Hormones(FSH, LH). Also the study showed the role of coffea arabica to induce the infertility through it's impact on the thyroid gland which responsible for the oogenesis and spermatogenesis.the result also showed that the high dose vitamin E working on the accumulation of free radical causing oxidative stress damaging body.

العلاقة المحتملة بين مرض الاكياس المائية وسرطان الكبد والرئتين == The Probable Relationship Between The Hydatidosis And Liver And Lungs Cancer

اسم المؤلف: ايمان سلمان خميس محمود

اسم المشرف: ندى محمد طه البشير | نغم ياسين البياتي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: تعد الاصابة ببعض العوامل المرضية مسؤولة عن حوالي خمس حالات السرطان في العالم. وهنالك العديد من الاصابات الطفيلية التي ثبت دورها كمسبب او محفز لسرطانات معينة، الا ان العلاقة بين الاصابة بالاكياس المائية والسرطان مازالت موضع جدال ولذلك فقد هدفت الدراسة الح | Infectious agents are responsible for about one fifth of all cancer cases worldwide. Some parasitic infections are well documented to cause certain cancers. However, the association of hydatid cyst (HC) with cancer is a controversial issue. The current study aimed to investigate the possible association between chronic hydatid cyst infection with liver and lung cancers. The study involved three groups : the first group included 42 patients with HC, the second group included 35 patients with liver (12) and lung (23) cancers, while 25 apparently healthy individuals were recruited to represent the third group (control). Number, diameter, location and the status (calcified or non - calcified) of the HC were recorded from HC group. Blood samples were collected from the studied groups from which serum were separated and kept until being used. Enzyme linked immune - sorbent assay (ELISA) was used to estimate serum levels of anti - HC IgG antibodies, carbohydrate antigen (CA19 - 9), alkaline phosphatase (ALP) and ? - glutaml transferase (GGT), while Absorbance measured by spectrophotometric method was used to determine levels of lactate dehydrogenase (LDH) and adenosine deamimase (ADA).All members of HC group gave positive result for anti - HC IgG antibodies compared to 11 patients from cancer group and oneindividual from control group (Odds ratio =8.3). Both HC and cancer groups showed significantly higher p<0.05 levels of CA19 - 9, ALP, LDH and GGT ((76.16±58.26 U/ml and 110.77±96.47 U/ml), (131.81±76.65 U/L and 141.72±97.23 U/L), (244.24±117.94 U/L and 254.68± 115.56 U/L) and (95.06±66.73 U/L and 105.12± 41.42 U/L) respectively) than control group (10.81± 9.12 U/ml, 65.14± 25.21 U/L, 146.15± 37.59 U/L and 17.21± 6.87 U/L respectively). In cancer group, casp - 8 level (72.20±54.72 picomol/ L) was significantly lower from than that of control group (92.30± 41.20 picomol/L) and insignificantly from that of HC group (60.86±40.84 picomol/L). In HC group, ADA level was 47.84±10.65 U/L and was significantly higher p<0.05 than that of cancer group (10.14± 1.99 U/L) and control (16.09±2.70 U/L).Investigation for the risk factors which may increase the risk of developing liver or lung cancers in HC patients revealed that male, smokers and rural residents were more susceptible for such development (Odds ratios : 3.45, 6.982 and 5.48, respectively). Number and cyst size were significantly correlated with the level of anti - HC IgG antibodies (r= 0.539, P<0.001 and r= 0.618, P< 0.001 respectively), while calcified cysts associated with significantly higher levels p<0.05 of CA19 - 9 and GGT (105.03±88.9 U/ml and 145.46±101.57 U/L, respectively) than that of non - calcified cysts(65.91±39.87 U/ml and 77.17±7.23 U/L, respectively). Average level of anti - HC IgG antibobodies in calcified cysts was 19.75±13.11U/ml and was significantly P<0.05 lower than its counterpart of non - calcified cysts (85.017±76.84 U/ml). All studied parameters showed significantly higher levels in livers cysts than their counterparts in lung cysts. Results of the current study indicate that chronic infection with HC can increase the host susceptibility to develop liver or lung cancer.

دراسة فعالية المشتت الحيوي السطحي المستخلص من بكتريا Bifidobacterium spp في تثبيط الغشاء الحيوي للمسببات المرضية المعزولة من مرضى القسطرة القلبية وتاثيره في عملية البلعمة == Study of The Effectiveness of Biosurfactant Extracted From Bacteria Bifidobacterium Spp. In The Inhibition of Biofilm of Pathogens Isolated From Cardiac Catheterization Patients And Its Effect In Phagocytosis

اسم المؤلف: بتول شاكر عبد المجلاوي

اسم المشرف: هيام عبد الرضا كريم العواد | علي رحيم حنظل الهامل

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة كربلاء - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: كربلاء

الصفحات الاولى:

المستخلص: This study was conducted in order to assess the effect of Biosurfactant extracted from bacteria Bifidobacterium spp in the inhibition of Biofilm produced by pathogenesis bacterial isolated from patients with during cardiac catheterization, This study getting 89 patients with cardiac catheterization unit in AL - Imam AL - Hussain Teaching Hospital from both genders of different ages ranged between (29 - 75) years, starting from (January 2014 and to the end of July 2014). The samples taken from atherosclerosis patients were cultured for all types of cardiac catheterization by three tests Swabs, catheter tip culture and blood culture sample before and after a catheter for the purpose of isolating pathogens that component of the biofilm of patients with atherosclerosis. The results of the current study showed that patients with diagnostic cardiac catheterization rate of 66 (74.16%) patients were given 15 (60%), 15 (60%), 14 (58.33%) and 15 (62.5%) bacterial growth, respectively. But patients with therapeutic cardiac catheterization of 15 (16.85%) patients were given 6 (24%), 6 (24%), 6 (25%) and 6 (25%) bacterial growth respectively, and diagnostic and therapeutic cardiac catheterization for together 8 (8.99 %) patients were given 4 (16%) 0.4 (16%), 4 (14.67%) and 3 (12.5%), bacterial growth, respectively. The results showed sensitivity and specificity values for swabs test 96%, 100%. while the catheter tip culture was 96% and 100% respectively, and blood culture was 100 and 98%, respectively, it became clear from the results after the sample cultivations on the enrichment and differential media that 89 patients (sample joint) gave 25 (28.08%) positive sample for bacterial cultivation, and two of them showed two types of bacteria to become 27 isolated bacterial, isolates were diagnosed 11 (70.74%) gram positive, 15 (55.56%) gram negative , one isolate only of the yeasts 1 (3.70%), and 64 (71.91%) did not give any growth. Ability of pathological isolates were tested for the production of Biofilm by using three different methods, tube method TM, Congo Red Agar CRA, microtiter plate M TP were given 25 (92.6%), 16 (58.26%) and 26 (96.29%) the isolate of producing biofilm by different quantities respectively. The results of the investigation of formation biofilm test showed that MTP has 100% sensitivity and 96% specificity, the TM sensitivity was 96% and 100% specificity and CRA was sensitivity of 61.5% and 100% specificity. One hundred and fifteen samples were collected from of local and imported dairy products 45 (39.13%), breast milk 32 (27.83%) and cows milk, 38 (33.04%) were obtained at 22 (19.13%) positive sample of Bifidobacterium bacteria after morphological, microscopic and biochemical diagnosis tests and comparing these isolates with standard isolates. Oil Spreding Techingue and blood Hemolysis was performed to investigate the capability Bifidobacterium on the production biosurfactant, the efficiency antithesis test was studied of liquid bacterial farm for Bifidobacterium bacteria against bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, E coli, Klebsiella pneumoniae , and Serratia marcescens was diameters of inhibition zones (24.25, 21, 24, 22, 23) mm respectively. The inhibitory effectiveness was Studied for filtrate against bacteria Staphylococcus epidermidis, S.aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, P.aeruginosa, Enterobacter Cloacae and Serratia mercescens, where the E.coli bacteria was more effective and P.aeruginosa less effective by the bacterial filtrate. The study included the effect of three concentrations of biosurfactant against bacteria test group, and the diameters of inhibition rates of inhibition areas to concentrations 50% as follows (33.34, 30.34, 32.30, 33.32) mm respectively. The diameters rates of zones of inhibition for the concentration of 25% was as follows (30.32, 27.32, 31.28, 31.5, 29) mm respectively, and zones inhibition rates of concentration of 12.5% as follows (28.29, 24.30, 26.26, 28.26) mm respectively, statistically significant differences at the significance level of P <0.01inhibition in rates depending on the type of user concentration and statistically significant differences between the types of bacteria in their affected by biosurfactant. also determine the values of (MIC) Minimum Inhibitory Concentraction, (SubMIC) Sub Minimum Inhibitory Concentraction and (MBC) Minimum Bactericidal Concentraction, the value of MIC 6.25 mg / ml, Sub MIC 3.12 mg / ml and MBC 12.5 mg / ml of the bacteria E.coli, K. pneumoniae, Enterobacter cloacae, and the value of MIC amounted to 12.5 mg / ml, Sub MIC 6.25 mg / ml and MBC 25 mg / ml of bacteria S.aureus, S.epidermidis, P. mirabilis, P. aeruginosa and Serratia mercescens. The susceptibility of biosurfactant was studied to inhibit biofilm bacteria by MTP method The results showed a significant decrease in the formation of biofilm after the addition of biosurfactant compared with the biofilm before adding biosurfactant where the decreased rate reached 96%. Effect of biosurfactant test was performed on the efficiency of phagocytosis in vitro the percentage of phagocytosis reached 40.9% of the control group 64.8% of the experimental group. Concluded that the biosurfactant has a clear inhibitory efficacy against pathogens isolated from patients with cardiac catheterization and inhibition of biofilm for pathogens as well as raising the efficiency of the process of phagocytosis in vitro

اسم المؤلف: غزوان مهدي جعفر الربيعي

اسم المشرف: نغم ياسين كاظم البياتي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية في محافظة ديالى للفترة من بداية شهر ايلول لغاية نهاية شهر تشرين الثاني لعام 2014. تم جمع 40 كيسا عدريا من الاغنام والابقار (34 عينة من الاغنام و6 عينات من الابقار). تم اجراء التحليل الكمي لبعض العناصر والمركبات الكيميائية فضلا عن الا | The current study was carried out in Diyala province during the period from September to December 2014. Fourty hydatid cysts were collected from sheep and cows (thirty four cyst from sheep and six cysts from cows). Quantitative analysis was done for some elements and chemical composition of hydatid cyst fluids, laminated layer of cysts and the tissues surrounding cysts isolated from lungs and livers of cows and sheep, in addition to the adipose tissues of sheep. The results revealed that there was no significant difference (p>0.05) in Na, Ca, K, Mg and Fe elements in the fluids of hydatid cysts isolated from livers and lungs and adipose tissues of sheep and cows, while in sheep, Na showed (p>0.05) the highest concentration (4.83±0.19 mg/dl) in lung tissues comparison with the adipose tissues and livers (4.73±0.10 and 4.57±0.17 mg/dl respectively). The highest concentration of Fe (p?0.001) was in liver tissue (3.80±1.28 mg/dl). In addition, the highest concentration of K (p?0.001) was in walls layers of hydatid cysts isolated from liver (3.61±0.36 mg/dl) comparison with lung and adipose tissue (3.60±0.39 and 2.64±0.21 mg/dl, respectively). In cows, the highest concentration of Na (p?0.05) was recorded in lung tissue (3.85±0.84 mg/dl) comparison with liver, while the highest concentration of K (p?0.05) was in liver tissue (5.40±0.27 mg/dl) in comparison with lung tissue (4.76±0.20 mg/dl). The highest concentrations of creatinine, cholesterol and triglycerides were recorded (p?0.001) in hydatid fluids of cysts isolated from liver (47.24±2.25, 8.29±0.83 and 0.34±0.01 mmol/l, respectively) in comparison with the fluids of hydatid cysts isolated from lungs (17.80±1.79, 0.43±0.36, 0.12±0.00 mmol/l, respectively). In addition, the highest concentrations of urea, total protein and glucose were recorded (p?0.001) in fluids of hydatid cysts isolated from sheep lungs (2.15±0.03, 0.68±0.02, 31.41±0.72 mmol/l, respectively). In cows, the highest concentrations of creatinine, cholesterol and triglycerides were found in fluids of hydatid cysts isolated from lungs (13.24±2.02, 0.12±0.00, 0.12±0.00 mmol/l, respectively) in comparison with those isolated from liver. In addition, the concentration of glucose in the fluids of hydatid cyst isolated from cow lungs was higher than in those isolated from the liver (0.62±0.00, 0.45±0.00 mmol/l, respectively). The current results revealed that the fluids of hydatid cysts isolated from liver showed (p?0.001) the highest concentrations of amino acids {proline (98, 82±3.07), tyrosine (171.15±4.9) and lysine (25.39±9.29) mg/dl), and (p?0.05) for methionine (91.92±1.11), cysteine (68.97±1.61), phenylalanine (21.53±10.80) in comparison with fluids of hydatid cysts isolated from lungs and adipose tissues. For adipose tissues, (p?0.001) the highest concentrations of glutamate (58.48±6.61 mg/dl), glutamine (53.53±4.96 mg/dl), serine (57.30±1.04 mg/dl), histidine (41.64±8.71 mg/dl), threonine (52.81±5.14 mg/dl), alanine (109.81±5.39 mg/dl) and leucine (34.81±1.30 mg/dl) in comparison with fluids of hydatid cysts isolated from livers and lungs. The arginine and valine (p?0.05) were the highest in the fluids of hydatid cyst of lungs (24.39±4.27, 100.72±10.82 mg/dl, respectively) in comparison with those isolated from the livers and adipose tissues. As for the concentration of amino acids in the surrounding tissue of hydatid cysts, the hepatic tissue recorded (p?0.001) the highest concentration of acids aspartic (105.52±5.14, glutamic (35.43±9.46 mg/dl) glutamine (114.72±5.02 mg/dl) Glycine (219.58±8.59 mg/dl) serine (32.97±1.10 mg/dl) arginine (47.90±1.04 mg/dl) threonine (56.03±3.94 mg/dl) alnin (17.74±4.74 mg/dl) proline (141.89±5.84 mg/dl) valine (202.81±8.45 mg/dl) methionine (225.99±5.72 mg/dl) sistine (109.39±3.61 mg/dl) Isoleucine (28.13±3.23 mg/dl) phenylalanine (77.92±2.93 mg/dl) respectively, compared with the tissue of the lung and adipose tissue. While recorded (p?0.001) the lung tissue highest concentration of acid histidine (40.94±2.65 mg/dl) and lysine (52.88± 2.16 mg/dl) mg/dl, respectively, compared with the liver tissue and adipose tissue. As for leucine (114.46±3.27 mg/dl) recorded (p?0.001) in adipose tissue highest concentration him compared with liver and lung tissues. As for the concentration of amino acids in the cyst wall of the of hydatid cysts, the cyst wall recorded (p?0.001) isolated from hepatic tissue highest concentration of acids (aspartic (261.38±8.80 mg/dl) glutamine (270.75±1.75 mg/dl) histidine (106.04±5.43 mg/dl) glycine (129.18±5.08 mg/dl) arginine (39.77±2.51) threonine (30.43± 4.63 mg/dl) alnin (30.54±3.25 mg / dl) methionine (182.50 ±1.50 mg /dl) proline (36.29±9.54 mg/dl) sistine (199.49±6.33 mg/dl) valine (154.63±6.98 mg/dl) Isoleucine (66.10±7.71 mg/dl) leucine (104.22 ± 3.94 mg/dl) phenylalanine (137.62±1.32 mg / dl) respectively, compared with cyst wall isolated from lung tissue and adipose tissue. As for the Lysine (33.62±2.5 mg/dl) recorded (p?0.001) highest concentration in the cyst wall isolated from lung tissue compared with cyst wall isolated from the liver and adipose tissue. As for the tyrosine (254.21±3.84) and glutamic (57.49±1.75) recorded (p?0.001) the highest concentration of them in the cyst wall isolated from adipose tissue compared with cyst wall isolated from lung tissue and the liver. For the concentration of amino acids in the fluids of hydatid cysts isolated from cow liver and lungs, the results showed (p?0.05) that the highest concentrations of alanine (85.88±1.42), tyrosine (21.41±0.39) and methionine (170.47±1.19) were recorded in fluids of lung cysts in comparison with the fluids of liver hydatid cysts. In contrast, there were no significant differences (p?0.05) in the concentrations of (aspartic, glutamic, glutamine, glycine, serine, histidine, arginine, threonine, proline, valine, sistine, leucine, phenylalanine, alnin and lysine between the hydatid fluids isolated from cow liver and lungs. As for the concentration of amino acids in the surrounding tissue of hydatid cysts cows show (p?0.05) that amino acids aspartic (288.47±10.13 mg/dl) and glutamine (270.79±2.97 mg/dl) recorded highest concentration in liver tissue compared with lung tissue. While recorded amino acid (p?0.05) alanine (230.58±1.38 mg/dl) proline (124.87±3.53 mg/dl) tyrosine (257.29±1.76 mg/dl) Isoleucine (296.44±2.06 mg/dl) and leucine (222.44±4.46 mg/dl) the highest concentration in the lung tissue compared with liver tissue. While no significant difference did not recorded in the other amino acids. As for the concentration of amino acids in the cyst wall showed cyst wall isolated from the lung tissue (p?0.05) of the highest concentration of acids valine (135.89±1.01 mg/dl) and leucine (169.65±2.64 mg/dl) compared with cyst wall isolated from hepatic cyst. While no significant difference did not recorded in other amino acids.