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دراسة بعض المؤشرات الوراثية الخلوية لتاثير مادة الكلوروفورم على الفار الابيض Mus musculus L == A Study on Some Cytogenetic Indices for The Effect of Chloroform on The White Mouse Mus Musculus L

اسم المؤلف: عمر رحيم خلف

اسم المشرف: عادل فوزي شهاب

الموضوع العام: علوم الحياة

السنة: 2008

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة تكريت - كلية العلوم - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: صلاح الدين

الكلمات الدلالية:

الحيوان - الوراثة

الصفحات الاولى: 📎 24T2843 - p.pdf

المستخلص: يستخدم الكلوروفورم على نطاق واسع في العديد من الصناعات مثل صناعة الورق والمطاط ومطافئ الحريق ومستحضرات التجميل ومواد التخدير. كما يستخدم كمذيب عضوي للعديد من المواد في المختبرات التعليمية والصناعية. وينتج الكلوروفورم من العمليات الايضية التي تقوم بها بعض | Chloroform is widely used in many industries, such as paper, elastic, fire extinguisher, cosmetics and anesthetic materials industries. It also used as an organic dissolvent for many materials in industrial and education laboratories. Chloroform is produced by The metabolism process of living organisms such as macroalgae and some fungus of highly organic matter soil. It can also be produced by process of water cholornation. Because of chloroform is widely used, and few studies achieved on cytogenetic level. Therefore The present study was conducted to identify The effects of chloroform on cytogenetic characteritis in laboratory mice.This study includes : 1 - A Study on Some Cytogenetic Indices For The Effect of Chloroform on The White Mouse.2 - Sperm abnormalities of male mice.The results of The present study showed that The chloroform has the ability to motivate chromosomal aberrations which were represented by (ring chromosom, chromatid break, and centromore break) in somatic cells in both males and females mice and in germ cells in males, The results revealed significant differences in The chromatid break and ring chromosome of both male and females mice, which injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with control group. It was found That there were asignificant differences in centromore break of females, injected with two concentrations of chloroform in comparison with control group, while non significant were differences observed in The males.Also the present study showed there were significant differences in The chromosomal aberration in The germ cells of males mice, injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with controlled group.The concentration (0, 05 mg/kg) was more influent in comparison, with The concentration (0, 025mg/kg). It was found that 30 days of injection is more influent in comparison with 15 day of injection, and males are more influenced than females.The present study recorded a significant differences in creating micronuclei in both male and female mice, injected by chloroform with a concentrations (0, 025 & 0, 05 mg/kg)in compared with control group.The result also revealed that The chloroform cause a significant decrease in The mitotic index of The somatic cells in both males and females, morever significant decrease in The mitotic germ cells of The injected males mice was found.An increase in The Blast index factor in The somatic cell in both male and female mice, injected by chloroform when compared with control group was similar result was recoded rgarding The Blast Index factor of The males germ cells. Regarding The sperm abnormality, the result showed significant differences in The sperm abnormality of males injected by chloroform with two concentrations (0, 025 & 0, 05 mg/kg) in comparison with The control group. The concentration (0, 05 mg/kg) was more influent than The concentration (0, 025mg/kg). Besides and the period 30 days of injection was more influent than the period 15 days.