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تاثير اضافة بعض الاحماض الامينية وعصير البنجر الى المخففات في بعض صفات السائل المنوي المبرد والمجمد للكباش == Effect of adding some amino acid and Beet juice to the dilutors on some cooling and freezing rams semen traits

Author name: فلاح عبد المحسن عبد الله الاسدي
Supervisor name: عماد فلاح حسن | عبد الرزاق نعيم خضير
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted at the agricultural experimental Research Station / College of Agriculture / University of Basrah form 3rd /1/2015 to 3rd /1/2016. A total of 12 mature rams (4 Najdi , 4 Arabi and 4 crosses) aged 3 - 3.5 years with nearly similar weights . Nutrition depended on concentrate ration ( 47 % wheat bran , 50 % barely ,1% urea , 1% CaCo3 and 1% Nacl ) , with 16.58 % crude protein and TDN 72.17% and was given 0.5kg /animal twice aday (at morning and afternoon)500 - 750 g / animal green fodder with wheat straw was given as it was available in the farm , cubic mineral and water provided freely at the day. Rams were training for semen collection for 30 days before the beginning of the study by using artificial vagina used for sheep. Semen was collected three time each month. The study includes two experiments : First experiment : It was designed to study the effect of breed , season ( spring , summer , autumn and winter ) and month on semen characteristics physical , sexual hormones ( testosterone , ICSH and SSH) of blood serum and level of enzymes ( AST , ALT and ALP ) of blood plasma.Second experiment : It included ten diluters from local materials (Beet pulp juice ) with 5 , 10 and 15% egg yolk and amino acids ( Glycin and cysteine ) with concentration of (5, 10 and 15) mmol. It also included the effect of type of dilutor , cooling , freezing storage period ( one and two months) in liquid nitrogen ( - 1960 C) on individual motility and live sperm percent .bThe results can be summarized as follow : 1 - Arabi rams exceeded (P<0.05) Najdi and crosses rams in their semen pH , spring also showed higher (P<0.05)pH followed by winter in comparison with summer and autumn .May showed higher (P<0.05) semen pH in comparison with other months.2 - Najdi rams exceeded (P<0.05)Arabi and their crosses in mean ejaculate volume, mass and individual sperm motility , sperm concentration , live sperm percent and mean testes circumference .3 - Summer and autumn showed higher (P<0.05) in meanejaculate volume , mass and individual sperm motility , sperm concentration , live sperm percent and mean scrotal circumference than winter and spring . Spring also showed higher (P<0.05) dead sperm percent followed by winter in comparison with summer and autumn .4 - August showed higher (P<0.05) ejaculate volume and mass and individual sperm motility followed July and June in comparison with other months. September , August and October exceeded (P<0.05)in comparison with other months sperm percent . October showed higher (P<0.05) in live sperm percent in comparison with other months . Highest (P<0.05) dead sperm percent happened during March followed by April and May . Scrotal circumference was high (P<0.05) during September followed by November and October.5 - Najdi and Arabi rams showed significant (P<0.05)differences in testosterone and ICSH hormones in comparison with their crosses . However , crosses exceeded (P<0.05)Arabi and Najdi rams in mean of SSH hormone. Autumn followed by summer exceeded (P<0.05) wintercand spring in testosterone , ICSH and SSH hormones. September and October showed higher (P<0.05)testosterone, ICSH and SSH hormones in comparison with other months.6 - Arabi rams exceed (P<0.05) both Najdi and their cross by enzymes concentration ( AST, ALT and ALP) of semen plasma . Spring and winter exceeded (P<0.05) summer and autumn by mean of ALT and AST enzymes. However , summer and winter exceeded (P<0.05) autumn and spring by mean of ALP concentration . March showed highest (P<0.05)AST and ALT concentration , while August showed highest (P<0.05) ALP concentration.7 - Amino acid diluter ( 15 mmol glysin and cysteine ) and 10 % beet pulp juice showed highest (P<0.05) individual motility after cooling and freezing for month and two monthsduring all months of year. While the beet pulp juice 15% and control showed the lowest means (P<0.05) of individual motility after cooling and two months of freezing.8 - Glysin 10 and 15 mmol , cysteine 10 mmol and beet juice 10% showed significant (P<0.05) increase in live sperm percent after and cooling and freezing . On other hand beet pulp juice 15% and control recorded the least value (P<0.05) . Beet pulp juice and control showed the highest (P<0.05) level of dead sperm percent after cooling and freezing in contrary with glysin 10 mmol and cysteine 15 mmol

تاثير الاحلال الجزئي او الكلي للحنطة المخمرة محل الذرة الصفراء في بعض الصفات الانتاجية والفسلجية لفروج اللحم == Effect of Replaced Partial or Complete Fermented Wheat Instead of Corn on some Productive and Physiological Traits of Broiler Chicks

Author name: علي جبر حمود علي الحميداوي
Supervisor name: عبد الله عبد المنعم محمد | جعفر محمد جاسم
General topic: Agricultural sciences
Specific topic: Zoology - Nutrition
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study included four experiments, two test laboratory experiments and two field experiments. The first experiment involved the use of laboratory quantities of inoculums dose and different periods of fermentation on the local wheat fermented with Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor to obtain the best percentage of crude protein with less fiber content between different fermentation methods. The seconds experiment depended of the best results of the first experiment and re - fermentation process with the addition of urea by 2% to provide a source of nitrogen for use by microorganisms to protein syntheses. The first field experiment was to see the effect of substitution of fermented wheat with Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor partially or completely replace corn in diets on the productivity of broiler chickens. Digestion experiment was to determine the proportion of digested crude protein and crude fiber in the various diets also conducted. In this experiment 360 unsexed chicks of broiler class (Ross 308) is distributed over eight treatment by 3 replicates per treatment (15 chicks for replicate) as follows. T1 and T2 : diets control with or without antibiotic respectively; T3 and T4 : substitution partial and complete of fermented wheat with Saccharomyces cerevisiae; T5 and T6 : substitution partial and complete of wheat fermented by Iraqi probiotic; T7 and T8 : treatment replace partial and complete rumen liquor fermented wheat. As the second field experiment designed to study the replacement of wheat fermented with Saccharomyces cerevisiae and Iraqi probiotic and rumen liquor with urea partially or completely replace corn in diets on the productive performance of broiler chickens as well as digest it conducted a second test after the end of the second field experiment. In this experiment, A 450 chicks were used and distributed over 10 treatments each with three replicates (15 chicks for replicate)Btreatments are : T1 and T2 partial and complete replacing of wheat without urea instead of corn, respectively (controls); T3 and T4 partial and complete replacing of wheat with urea instead of corn, respectively (controls); T5 and T6 partial and complete replacing of wheat fermented by Saccharomyces cerevisiae with urea instead of corn, respectively; T7 and T8 partial and complete replacing of wheat fermented by Iraqi probiotic with urea instead of corn, respectively; T9 and T10 partial and complete replacing of wheat fermented by rumen liquor with urea instead of corn, respectively. The current studies included the following results : The first laboratory experiment : Fermented wheat by Saccharomyces cerevisiae with the amount of inoculums get 2 gm. - 1 Kg of wheat and 14 days fermentation period the archieved highest percentage in crude protein, followed by treatment of the fermented wheat rumen liquor with the amount of inoculums dose of 4 ml. Kg - 1 wheat and fermentation period of 14 days and then the treatment of Iraqi probiotic fermented wheat 5 gm. Kg - 1 and fermentation period of 21 days to access to 14.64% ,14.43% and 13.59%, respectively, in the treatment of non - fermented wheat recorded of 11.59% crude protein.The second laboratory experiment : The seconds experiment depended three of the best results of the first experiment (Fermented wheat by Saccharomyces cerevisiae with the amount of inoculums get 2 gm. - 1 Kg of wheat and 14 days fermentation period, fermented wheat by rumen liquor with inoculums dose of 4 ml. Kg - 1 wheat and fermentation period of 14 days and fermented wheat by Iraqi probiotic 5 gm. Kg - 1 and fermentation period of 21 days) re - fermentation process with the addition of urea by 2%.CThe first field experience : fermented wheat treatments by Saccharomyces cerevisiae, Iraqi probiotic and rumen liquor increased significantly (P≤0.05) the live body weight, total weight gain, accumulative feed intake, economic figure, percentage of bone ash, villi height, ratio of villi height to crypt depth of the intestine, number of red blood cells, percentage of hemoglobin as well as the level of calcium and cholesterol in serum recorded significant decrease in favor of fermentation treatments were favorable results for the benefit of the treatment of substitution of wheat fermented by rumen liquor (T8) significantly (P≤0.05) was showen in protein and fibers digestibility in T7 and T8. While no significant defferances recorded in feed conversion ratio, viability, dressing percentage, organs weight, sensory characteristics, intestinal length and crypt depth were not different among all including the control.The second field experiment : The results showed that there were significant increases in final live body weight, total weight gain of T5, and accumulative feed intake for the T6 group, while T10 recorded the best conversion ratio, also T9 and T10 recorded the best economic figure, dressing percentage, percent of hemoglobin and packed cell volume. and Femur bone. As well as best protein and fibers digestibility were recorded as compared with control. Six treatment was showed the best results in blood serum ELISA titer against for Newcastle disease, infection bursa disease, Avian influenza and Bronchitis while non significant difference showed in viability, gizzard and proventiculus percent

التشكل الوراثي لجيني اللاكتوفرين والبرولاكتين وعلاقته بانتاج الحليب ومكوناته واداء الحملان قبل الفطام في النعاج العرابية == Relationship between Lactoferrin and Prolactin Genes Polymorphism and Milk Yield and its Components and Pre - weaning Lambs Performance of Arabi Ewes

Author name: علي جاسم عبد الرضا
Supervisor name: اسعد يحيى عايد | سمير اسطيفان حنا
General topic: Agricultural sciences
Specific topic: Zoology - Education
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Polymorphism analysis of lactoferrin and prolactin genes and its relationship with milk yield and its components and birth, weaning weight and growth rate before weaning were studied. DNA was extracted from 77 blood samples of Arabi ewes (21 ewes from Animal Farm, College of Agriculture, University of Basrah and 56 ewes from Animal Farm, College of Agriculture, University of Al - Muthana). Lactoferrin genotypes were determined by using Hind III restriction enzyme, those of prolactin by Hae III enzyme at Genetic Engineering Laboratory/ College of Agriculture/ University of Basrah and College of Animal Resources/ Mullah Thani University/ Islamic Republic of Iran. Milk yield was measured once a week from 52 ewes belong to Animal Farm/ College of Agriculture/ University of Al - Muthana. Samples were chemically analyzed. Birth and weaning weight were recorded as well as growth rate before weaning during a period of 1/2/2013 to 31/12/2013. Results revealed the following : 1 - The possibility of amplifying both genes and get two alleles for each and three genotypes of the lactoferrin gene (AA and AB and BB) and two genotypes (AA and AB) of the prolactin gene.2 - Prolactin and lactoferrin A allele frequencies were 0.94 and 0.66 respectively. AA genotypes frequency was 0.88 and 0.52; AB 0.12 and 0.29 and BB 0.00 and 0.19 of prolactin and lactoferrin genes respectively. population was at equilibrium for prolactin gene, but was not in lactoferrin gene.3 - Heterozygosity levels were 11.69% and 28.57% for prolactin and lactoferrin genes respectively. inbreeding coefficients (Fis) were - 0.05 and 0.345 for both genes respectively. University of Basrah and Al - Muthana herds showed very high genetic correlation (0.999).4 - The overall means of total and daily milk yields and lactation length were 61.13±2.76 kg, 0.582±0.04 kg and 104.75±1.95 days respectively. Ewe age and lamb sex did not significantly influence the above traits. However, ewes lambed during February exceeded thoselambed in September by daily milk yield (0.661 vs 0.577 kg) and lactation period (110.00 vs 100.00 days).5 - The overall means of total fat, protein and SNF and their percentages were 2.65 Kg and 4.33%, 2.5kg and 4.11% and 11.98% respectively. Only SNF was significantly influenced by ewes age and lamb age.6 - The overall means of birth and weaning weights and pre - weaning daily growth rate were 4.48±0.39 kg, 21.21±0.89 kg and 161.71±8.47 gm./day respectively. Lambs birth weight was significantly influenced by ewes age, ewes aged 4 and 5 years got heavier lambs than those aged 1 and 3 years.7 - Lactoferrin genotypes significantly affected protein and total solids not fat, as AA genotype exceeded the other genotypes. Fat of AA, AB and BB genotypes were 2.85, 2.23 and 2.63 kg respectively. while values of SNF were 7.24, 5.78 and 7.51 respectively.8 - There was arithmetic increase (not significant) in all traits for the AB genotype over AA genotype of prolactin gene.9 - Allele B of prolactin gene positively and significantly influenced most trait. It shared about 75% of each trait variation. That of lactoferrin gene shared about 25% only.10 - Heritability of SNF% and quantity was high (0.652 and 0.666 respectively). Those of birth weight and growth rate were medium (0.309 and 0.346). All other traits had low heritability.11 - Highest value of BLUP was recorded for daily milk yield, total milk yield and adjusted milk yield for 150 days (0.032, 3.072 and 2.220 kg respectively). The lowest values were - 0.021, - 2.071 and 0.00 kg respectively. Their ranges were 0.053, 5.143 and 2.220 kg respectively.12 - The lowest value of BLUP of fat%, protein% and SNF% were - 1.724, - 2.448 and - 4.565% respectively. the highest values were 2.058, 2.466 and 3.764% respectively with a range of 3.782, 4.914 and 8.329% respectively.13 - The lowest values of BLUP of birth and weaning weight and growth rate were 3.00 kg, 19.9 kg and - 1.20 g/day, the highest were 5.5 kg, 23.45 kg and 1.03 g/day with ranges 2.5 kg, 3.86 kg and 2.23 g/day respectively.14 - Breeding values of prolactin AB genotype of all studied traits exceeded those of AA genotype. However, those of lactoferrin AB and BB genotypes exceeded those of AA genotypes.

التعبير الجيني ووفرة نسخ mRNA لجينات نقل العناصر الغذائية في الامعاء الدقيقة لفروج اللحم باختلاف مستوى الطاقة والبروتين في العليقة == Gene Expression and mRNA Abundance of Nutrients Transporter Genes in The Small Intestine of Broiler of Different Levels of Protein and Energy in Diets

Author name: علي احمد عبد الكریم ابراهیم العامري
Supervisor name: اسعد یحیى عاید | عبد لله عبد المنعم
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted in both Poultry Farm/Animal Resources Department/ College of Agriculture / University of Basra and laboratories of Molecular Biology/ London/ United Kingdom (The Molecular Oncology Unit Laboratories of Guy's and St Thomas's Hospital/ Kings College London/ UK). Molecular analysis of the samples and the detection of a number of gene expression of several feed transporter genes in the small intestine of broiler chickens when fed diets with different levels of energy and protein. A total of 225 unsexed one day old broiler chicks (Ross breed) were randomly distributed on five experimental treatments each with three replicates of 15 chicks. Feeding was ad libitum during the whole period of experiment that lasted 35 days.The first treatment was control with 22.3 and 20 % crude protein and 2910 and 3001 kcal/kg for starter and growth period respectively. The second treatment consisted 24.1 and 22 % crude protein and 2920 and 2978 kcal/kg for starter and growth period respectively. The third treatment consisted 20 and 18 % and 2898 and 2996 kcal/ kg for starter and growth period respectively. The fourth treatment consisted 21.9 and 20 % and 3107 and 3199 kcal/ kg for starter and growth period respectively. The fifth treatment consisted 22.2 and 20 % and 2720 and 2800 kcal/ kg for starter and growth period respectively. The results revealed the followings : - The study showed the ability to amplify all genes and find their abundance according to their primers. - The highest value of APN gene abundance of female in the duodenum (1.86 ng) fed diet with 22 % cp and 3199 kcal/kg ME. - Highest abundance (6.19 ng) of Pept1 mRNA showed by males in their jejunum, when fed a diet consisted 22 % cp and 3199 kcal/kg ME. - The greatest value of b - AT and CAT2 amino acids transporter genes effect was revealed by males fed diet with 22 % cp and 3001 kcal/kg ME. - Females fed 20 % cp and 3199 kcal/kg ME diets reordered highest SGLT1 gene expression (4.70ng) within their ileum. - Males fed 20 % cp and 3199 kcal/kg ME diets reordered highest GLUT5 gene expression (6.37 ng) within their ileum. - All genes associated positively and significantly with body weight, growth rate, carcass cuts percentages (legs, breast, intestine length and weight) at the age of 35 days and negatively with feed consumption and feed conversion ratio. - Amino acid and peptides transporter genes abundance improved with an increase in diet protein, that of saccharide transporter improves with the increase of ME level in the diets. - Male gave higher mRNA copies of most genes in comparison with females, except that of SGLT1 and NPA which increased in females. - It is possible to manipulate nutrient transporter gene expression by feeding. That may reflect on birds performance.

استخلاص مضادات الاكسدة بمساعدة الموجات الدقيقة من اليرون حبة القمح وجنينها ونخالتها ودراسة تاثيرها في الصفات الريولوجية للعجين == Extraction of Antioxidant with Assisted Microwave from Aleuron Wheat Grain, Germ and Bran and Study its Impact in the Rheological Properties of Dough

Author name: علاء محمد صالح مجيد المسافر
Supervisor name: علي احمد ساهي | علي خضير جابر الركابي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to investigate and test the activity of three essential parts of wheat seed Aleurone, germ and bran which were used as secondary products from grinding grain to feed animals. diversity solvents (distilled water, chloroform, hexane, ethanol, methanol) were applied to extract the bioactive compounds by both traditional and modern extractions.The total phenolic compounds, antioxidant activity, reducing power, metal chelating, and scavenging of hydrogen peroxidase were determined for all three essential parts of wheat. The stability of the extracts was studied toward temperature and pH at the interval time. In addition, the synergistic effect of the extracts was studied by mixing with different concentrations of ascorbic acid and alpha tocopherol. The extracted compounds were identified using gas chromatography mass spectroscopy (GC - Mass).The ethanol extraction of wheat germ had the highest antioxidant activity, so it was chosen to prevent oxidation in the corn oil. Moreover, the ethanol extractions for germ, bran and Aleurone were added to wheat flour (80 % extraction) in order to study their effects as improvers in the rheological properties of wheat flour dough. The results were as follows : 1 - The microwave - assisted extraction for 40 sec was able to extract the highest level of phenolic compounds from wheat germ compared toconventional extraction conditions. The total phenolic compounds were 556.6 μg gallic/ml, 426 μg gallic/ml for ethanol and methanol extractions, respectively.2 - The microwave - assisted extraction was able to give the highest antioxidant activity (53%) at concentration of 100 mg/ml from ethanolic wheat germ extraction compared to synthetic antioxidant BHT ( 92 %). In addition, the wheat germ had the highest reducing power (163.88 %) at concentration of 5 mg/ml , while the percentage of reducing power for both ethanolic extraction of Aleurone and ethanolic extraction of wheat bran were 112.63 % and 79.68 %, respectively. In contrasts, all of above extractions had lowest reducing power compared to BHT and alpha tocopherol 249.8 % and 223.5 % respectively at the same concentration. Moreover, the wheat Grem had the highest percentage for both Ferrous chelating and scavenging of hydrogen peroxidase.3 - Ethanolic extracts of Aleurone, bran and germ have a higher antioxidant activity in natural pH while decreased when treated with acid and alkaline. In addition, the ethanolic wheat germ was able to give the highest antioxidant activity at 100°C for 100 min wich read 62.2% compared to others and gave synergistic effect for bothascorbic acid and alpha tocopherol exhibited 91.90 % of antioxidant activity at concentration 60 mg/ml.4 - Identification of ethanolic and methanolic extract using GC/MS showed presence of bioactive compounds such as ascorbic acid, benzoic acid, phytosterol compounds, Isoeugenol and alkylresorcinols5 - Ethanolic extracts of germ with concentration 0.05, 0.10, 0.15, 0.20, 0.25 mg/g was added to corn oil in order to compared with BHT 0.05% and stored at 45°C for 28 days, the result showed that the ethanolic wheat germ at 0.25 mg/g had the ability to decrease the hydrogen peroxide for the corn oil 6.8 meq/kg at end of stored time compared BHT (0.05 %) 4.3 meq/kg at the same conditions.6 - The ethanolic Aleurone extraction possessed a positive effect on the rheological properties when it was added as improvers to the wheat flour ( 80 % extraction). The dough consistency was 6.7 min, which considered highest reading among all treatments. In addition, the recorded elastic index was 3.2, 2, and 1.8 at interval times 45, 90, 135 min, respectively.

تاثير اضافة الخليط التازري لمسحوق حليب الكيفير المجفف والمصنع في بعض الصفات الانتاجية والفسلجية والمناعية لفروج اللحم == Effect of Addition of Synbiotic of Dried and Processed Kefir Milk Powder in Some of The Productive, Physiological and Immunological Characteristics of Broiler Chicken

Author name: عقيل طوينة عودة
Supervisor name: عبد الله عبد المنعم محمد
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Four experiments were carried out for this study, two field and two laboratory, to manufacture a Synbiotic for milk powder Kefir ( MPK ) and loaded with some feed material with or without sugar beet milled or Helianthus tuberosus powder and the effect of adding it to some of the production , physiological and immunological characteristics of the chickens . Where the first laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agricultur - University of Basrah , for the period from 10 / 4 / 2015 to 7 / 5 / 2015 for the purpose of manufacturing a Synbiotic of MPK.The second field experiment was conducted in a commercial field for poultry in Al - Zubair / Basrah during the period from 24 / 1 / 2016 to 27 / 2 / 2016 , which included selecting the best biomass of MPK loaded on wheat , corn , soybeans and wheat bran with the addition of Helianthus tuberosus powder , So that one gram of MPK and loaded on the previous materials can bacteria . In this L. acidophiluscfu / ml 8least 10be provided at experiment, 450 unsexed one day of the Ross 308 chicks, at a rate of 40 g / chick were distributed randomly to six Treatments , three replicates per treatment , 25 birds per replicate and the treatments were as follows ; T1 : Control treatment included a standard diet . T2 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach . T3 : A standard meal with 3 g of MPK loaded on wheat/ kg feed .T4 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T5 : A standard meal with 3 g of MPK loaded on soybeans / kg feed. T6 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed .The third field experiment was conducted to determine the best effect of the synbiotic MPK loaded with wheat, corn, soybeans and wheat bran and compared with three foreign probiotics in the productive, physiological and immunological characteristics of chicks. This experiment was conductedbduring the period from 4 / 10 / 2016 until 9 / 11 / 2016, using 600 unsexed chickens with one day of the Ross 308 at a rate of weight of 40 g / chick .The chicks were randomly distributed into eight treatments with three replicates per treatment and 25 birds per replicate. The experiment was designed as follows : T1 : Control treatment included a standard diet, T2 : A standard meal with 3 g of MPK loaded on wheat/ kg feed . T3 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T4 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed. T5 : added treatment 1/2 g of probiotic Labzyme / kg feed , Korean - made , T6 : added treatment 1/2 g probiotic Biozyme / kg feed , German - made , T7 : added treatment 1/2 g probiotic Biolac / kg feed , Vietnamese made , T8 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach .The fourth laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agriculture, University of Basrah for the period from 6 / 3 / 2016 to 7 / 4 / 2016 for the study of antibacterial activity in the kefir milk and compare it with Biozyme , Pencilin, Gentamycin, Tetracyclin and Neomycin against Staphylococcus aureus and Escherichia coli causing bacterial necrosis with Bacterial Chondronecrosis with Osteomyelitis ( BCO ) . The results of the study included the following : First laboratory experiment ;The results of the first experiment showed that the wet biomass rate of fermented kefir milk was 70 % . This high percentage is evidence of the growth of Lactate Bacteria in this product. The results showed that wheat bran and wheat bran and corn were the best loaded significant materials (p <0.05) Compared to soybeans in terms of growth and reproduction of lactic acid bacteria. Adding Helianthus tuberosus powder increased the number of bacteria in dried kefir milk compared sugar beet milled , which reduced the number of bacteria in MPK .Second field experiment : cThe results of the experiment showed the following : 1 - There were significant increase (p <0.05)) in the final body weight rate and the body weight gain and Food conversion efficiency, as well as a significant decrease (p <0.05) In the amount of feed consumed for the sixth, third and fourth treatments, supplemented with synbiotic MPK loaded on wheat bran, wheat and corn compared with other treatments .2 - Treatments sixth , third , fourth had significant (p<0.05) decrease mortality percent as comparing with control and treatment two .3 - The sixth , third , and fourth treatments were significantly higher (P <0.05) in the production index and the performance index and drcrease value of economic efficiency compared with other treatments .4 - Total protein and globulin in plasma serum were increased significant (p<0.05) and decreased (p <0.05) in the concentration of triglycerides and cholesterol in the sixth, third and fourth treatments supplemented with synbiotic MPK , loaded on wheat bran , wheat and corn , as well as the increased of the third, sixth and fourth treatments significantly (p <0.05) in the concentration of ALP enzyme compared to other treatments .5 - The results showed that the fifth, sixth, third, and fourth treatments did not differ significantly in antibodies titer against Newcastle ( NDV) and the Comboro ( IBDV ) disease, but significantly exceeded (p <0.05) compared to the first and second treatments .Third field experience : According to the results of the second field experiment, the best three treatments were added to the synbiotic MPK and loaded on wheat bran , wheat and corn . It was compared with three foreign prpbiotics , the treatment of addition of amoxicillin antibiotic and control treatment. The results of the experiment showed the following ;d1 - The second , fourth , third and sixth treatments, supplemented with probiotic, MPK , loaded with wheat , wheat bran , corn, and labzyme were significantly higher (p <0.05) in body weight and weight gain . As well as a significant decrease (p <0.05) in the amount of feed consumed and a significant improvement (p <0.05) in the efficiency of food conversion compared to other experimental treatments .2 - The first, fifth, and seventh treatments were significantly higher (p <0.05) in the mortality percent as comparing , to other experimental treatments .3 - A significant (p <0.05) increased was observed in the production index and the performance index and significantly decrease in the economic efficiency value for the second, fourth, third and sixth treatments.4 - The second, fourth, third and sixth treatments were significantly higher (p <0.05) in dressing percentage , tibia and thoracic characteristics , as well as a significant increase (p <0.05) in the weight of the cecum compared to other treatments.5 - There was a significant increase (p <0.05) in the weight of Bursa fabricius for the first, eighth and seventh treatments, as well as a H/L ratio was significant (p<0.05) decrease in the second, third, fourth and sixth compared to other treatments.6 - Total protein and globulin in plasma serum were significant (p<0.05) increased and decrease significantly(p <0.05) in the concentration of triglyceride and cholesterol in treatments second, fourth, sixth and third , compared to other treatments.7 - The superiority of the second, third, and fourth treatment was significantly higher (p <0.05) in the concentration of ALP enzyme in plasma serum compared to other treatments.8 - The third, seventh, second, fourth, and sixth treatments recorded the highest significant mean (p <0.05) of the degrees granted to them by theeEvaluator in the flavor, tenderness and juicy compared to the fifth, first and eighth treatments .9 - The second, seventh, third, fourth, and sixth treatments were significantly higher (p <0.05) in lactic acid in intestinal fluid , as well as There were significant (p<0.05) increase in digestibility of dry matter , protein and fat in treatments fourth, second, third and sixth as compared with other .10 - The fourth, second, third, and sixth factors significantly exceeded (p <0.05) in weight and length of the tibia , bone index , ash ratio , and skeletal weight of the chickens, as well as The second, fourth, third, and sixth treatments were significantly higher (p <0.05) in the level of calcium and phosphorus in the bone compared with other .11 - The first, eighth, fifth and seventh treatments were significantly higher (p <0.05) in the rate of food passage in the gastrointestinal tract compared with other.12 - Significant increase (p<0.05) in villi length and crups depth in treatments fourth, second and third as compared with seventh, fifth, eighth and first.Fourth laboratory experiment : The results of the experiment showed no significant differences in the treatment of MPK with gentamycin in diameter (mm) inhibition of Staphylococcus aureus, Escherichia coli, Which cause BCO disease , while significantly increase (P <0.05) with Pencilin, Labzyme, Tetracyclin , Biozyme in diameter of the inhibition zone to the staphylococcus aureus and the diameter of the inhibition zone of Escherichia coli.

تاثير فاصلة ومعاملات الري وتغطية سطح التربة في بعض خصائصها وانتاج نخلة التمر Phoenix dactylifera L. == Effect of interval and methods irrigation in addition soil surface mulching on some soil properties and production of date palm Phoenix dactylifera L

Author name: عبد الرحمن داود صالح الحمد
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in an agricultural field which located at Abu - AlKhaseeb distruct /20 Km south of Basra City , during the season of growth (2013 and 2014) on a land area about one hectare, a clay soil texture and classified as Typic torrifluvents. In order to study the effect of method and interval irrigation and mulching soil surface in some physical and chemical properties of the soil and production of date palm Phoenix dactylifera L. Hillawi c.v. The irrigation method factor included four treatments; drip irrigation method (D) and surface irrigation (S) and rotation method (DS) (rotation drip irrigation with surface irrigation in binary cycle) and tide irrigation method (T), while the mulching factor included three treatments : nylon (N) and coverage waste (W) and no mulching (no). Irrigation interval factor included three treatments are (4 ,8, 12) day. Experiment carried out in factorial Experiment with random complete block design (RCBD) with three replications, the addition of irrigation water calculated from the basin American evaporation (Evap.Pan Class - A - ), which was erected in the study site, plus 20% as leaching requirements.Below is a summary of the study results : 1 - The addition of irrigation water by the irrigation methods (D, DS, S) increase the moisture content significantly for different depths of soil compared with traditional irrigation treatment (tidal). Surface irrigation treatment shows higher values in the moisture content, followed by treatment of irrigation alternately then treated drip irrigation, irrigation with treated showed tidal lowest values. The irrigation methods (drip, drip - surface, surface) increased in moisture content values with lack of a period irrigation, especially when the interval 4 day. Moisture content of the irrigation methods treatments increased significantly values morally using coverage, particularly nylon except irrigation treatment tidal which were not increase significantly. The moisture content values increase significantly with depth for all experimental treatments, and she took to decline at the end of the first season and the second compared to the first season.2 - The treatment of traditional irrigation (tidal) showed the lowest values in the bulk density, followed by treatment of drip irrigation and treatment of rotation and then surface irrigation treatment for all depths .The results showed increased bulk density values of all treatments with depth ,also that the use of the nearby interval and coverage nylon contributed to maintaining the values of bulk density and showed superior in giving the lowest value for the density. Increased bulk density values with the progress of seasonal growth of all treatments and three depths of soil.3 - The use of irrigation methods (drip, drip - surface, surface) to a significant decrease in the values of Mean Weighted Diameter (MWD) compared with the traditional method of irrigation (tidal) and the order of the effect for the irrigation methods is S <DS <D <T. The results showed that values increase with the progress of the growing season, except for the treatment of traditional irrigation (T) with superiority of depth (0 - 30) cm on other depths.4 - The cumulative infiltration and infiltration rate Increased in the soil with the traditional irrigation treatment compared to using other methods of irrigation and the methods irrigation taken following order in the cumulative infiltration and infiltration rateS <DS <D <T. The results showed that the cumulative infiltration and infiltration rate values increased with using mulching especially with nylon and follow short - rang irrigation interval .5 - The use of irrigation methods (drip, drip - surface, surface) decreased the electrical conductivity values on different depths of soil compared with traditional irrigation treatment (tidal), which maintained a significant increase in the electrical conductivity values, especially in (0 - 30 ,30 - 60 ,) cm depth, followed by treatment of drip irrigation and surface irrigation, The rotation treatment DS showed high efficiency in salts washing rate in all depths and recorded the lowest values in the electrical conductivity .The results showed that the using of mulching, especially nylon contributed significantly in reducing the electrical conductivity values, and that the exposed treatment showed higher values, as the results showed that the using of long interval increase in the electrical conductivity values .Other than increase the electrical conductivity values of the surface depth of all treatments and it decreased significantly at both ends of the first and second seasons, compared with the beginning of the experiment except for the treatment of tidal which remained conservative values until the end of the experiment .6 - The use of irrigation methods (drip, rotation, surface) contributed to the significant increase of the values of productivity (weight, size, length, total sugars, dry weight and total yield) of Date Palm compared with the use of traditional irrigation method (tidal ) .The irrigation methods takes the following order in productivity : T <S <D <DS. The results showed that a significant increase in productivity with use short interval and the use of nylon mulching style. The results showed that increase in productivity in the second season compared to the first season.

تاثير رش التربتوفان والكلايسين واضافة المحفز الحيوي Biohealth للتربة في نمو وحاصل بعض اصناف البطاطا SolanumtuberosumL في الاراضي الصحراوية == EFFECT OF SPRAYING TRYPTOPHAN, GLYCINE AND SOIL APPLIED BIOHEALTH ON GROWTH AND YIELD OF SOME POTATO (SolanumtuberosumL.)CULTIVARS IN DESERT LANDS

Author name: عباس كاظم عبید عباس
Supervisor name: عصام حسین علي الدوغجي | نوال مهدي حمود
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Two experimentswere conducted during autum seasonsof 2013 - 2014 and 2014 - 2015 in Tomato Development Project belong to Basrah Agricultural Director, Khor Al - Zubairin southern Iraq to study the effect of foliar application of tryptophan, glycine and soil appliedBiohealthongrowth and yield of some potato cultivars. The aim of the experiments was focused on the effect of three potato cultivars (Draga, Arnova and Provento) in the first season and (Arizona, Arnova and Rivira) in the second season.Spraying with two concentrations( 100 and 200 mg.l - 1)for both Tryptophan and glycine and 0 mg.l - 1(sprying with distilled water).Spryingwas done twice 45 and 60 days after after propagating.Besides, additiontwo concentrations ofBiohealthinto the soil( 0 and 1 g.l - 1for the first seasonand 0 , 1 and 2 g.l - 1 for the second season). The addition ofBiohealthwas done twice 45 and 60 days after propagatingfor both seasons. The experiments was adopted as factional experiment.Split - Split Plot Design was used with three replication,Least Significant Differences Test(LSD) was used at probability of 0.05. The results could be summarized as follows : 1. Emergence of field parameters Results showed that"Arnova" cultivargave asignificant increases in speedemergence field after 20.51 and 19.43 daysin both season,respectively."Brovento"in first season and “Arezona” in the second seasongave asignificant increasesin the percent of field emergence 96.42 and 89.33%respectively, .2.Vegetative growth parameters Resultsshowed that"Draga"cultivar in the first season and "Rivira" inthe second seasonincreased significantly in mostvegetative growth parameters.Spryingwith 200mg.l - 1 tryptophan in bothseasons gave increased significantly in mostvegetative growth parameters.Application withbiohealth1 g.l - 1 in the first seasonand 1, 2 g.l - 1In the second season gave significant increases in mostvegetative growth parameters.The interactions between the studied factors caused significant increased effects in all vegetative growth parameters on both seasons. 3. Yields parameters Results in the first season showed that "Draga" cultivar gave significant increases in tuber number.plant - 1,total plant yield, marketable yield,total yield, small and large tubers yield (6.10 tuber,379.9g,3.36 ton.donum - 1, 3.71 ton.donum - 1,0.35ton.donum - 1 and2.52 ton.donum - 1,respectively), While "Rivira cultivar in the second season gave significantincreases in tuber weigh,total plant yield,marketable yield,total yieldand large tubers yield(88.38 g, 492.6g,4.50 ton.donum - 1,4.82 ton.donum - 1, 3.87 ton.donum - 1,respectively).Sprying with 200 mg.l - 1 tryptophan in the two seasons gavesignificant increases in tuber weigh (69.08, 74.95 g), total plant yield (389.1 , 438.4 g), marketable yield (3.54, 3.87ton.donum - 1),total yield (3.80, 4.28 ton.donum - 1) and large tubers yield(2.44, 3.08 ton. donum - 1) respectively, While the 100 mg.l - 1 tryptophan increases in tuber number.plant - 1 in the first season(5.76 tuber) and 200 mg.l - 1 tryptophan increases in the first seasonin yield medium tubers (1.10 ton.donum - 1)and in the second season increases in tuber number.plant - 1(5.90 tuber).Application with1g.l - 1 biohealthin the first season gave significant increases in tuber number.plant - 1,total plant yield,marketable yield,total yield,medium and large tubers yield(5.72 tuber, 366.6 g, 3.28 ton.donum - 1,3.59 ton.donum - 1,1.03 ton.donum - 1and 2.26 ton. donum - 1,respectively), while in the second season application with 2 g.l - 1 biohealthgave significant increases in tuber number.plantC 1,total plant yield,marketable yield,total yield,and large tubers yield(5.85 tuber, 423.4 g), 3.75ton.donum - 1 ,4.15 ton.donum - 1 and 3.00 ton.donum - 1,respectively). The interactions between the studied factors caused significant increased in allyields parameters on both seasons.4. Chemical characteristics 4 - 1. Leaves chemical characteristics Spraying with 100 and 200 mg.l - 1 tryptophan in Carbohydrate contents. In the second season200 mg.l - 1glycinegave significant increases in carbohydrate contents.Application with 1 g.l - 1biohealthin second season gave a significant increase incarbohydrate contents.The interactions between the studied factors caused significant increased effects in all leaves chemicalparameters on both seasons.4 - 2. Tubers chemical characteristics Results showed that "Brovento" cultivar in the first season gave significant increases in the percentage of dry matter, starch and specific gravity, "Draga" increases in thepercentage of protein, vitamin C and total solubilesoilds, "Arnova" increases intotal free amino acids. In the second season "Rivira" cultivar gave significant increases inthe percentage of dry matter, starch, specific gravity and total free amino acids and"Arezon" increases in thepercentage of vitamin C and total solubilesoilds.Foliar application with tryptophan at 200 mg.l - 1 in the first season caused an increase in the percentage of dry matter, starch,specific gravity and protein, while 200 mg.l - 1 glycine increases in vitamin C and total solubilesoilds, glycine at100 mg.l - 1increases in total free amino acids.Foliar application with tryptophan and glycine at 200 mg.l - 1in second season caused an increase in the percentage of dry matter, starch andspecific gravity, while 200 mg.l - 1 glycine caused increases in the percentage of protein and total free amino acids, glycine at100 mg.l - 1 increasesin vitamin C and tryptophan at 200 mg.l - 1 in total solubilesoilds.Application with biohealth gave asignificant increase in mosttubers chemical characteristics for both season.The interactions between the studied factors caused significant increased effects in all tuber chemicalparameters on both seasons.

استخلاص وتنقية وتوصيف متحللات بروتينية من مخلفات الاسماك والروبيان واختبار كفاءتها في حفظ اقراص مفروم اللحم البقري == Extraction, purification and Characterization of Protein hydrolysates From Fish and Shrimp by - Products and Assay Efficiency in Beef Patties Storage

Author name: عالية زيارة هاشم الحلفي
Supervisor name: ام البشر حميد جابر الموسوي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was interested in the preparation of bioactive peptides by using shrimp and fish by products.Three types of proteolytic enzymes , Alcalase and Pepsin and Flavourzyme were used for this purpose.The constituents (moisture , protein , fat and ash) of defatted raw and dried materials were studed .The proteolysis action of the three enzymes was observed for 5 hours the peptides chain length of the protein hydrolysates were determined and tested for their antioxidat and antibacterial 240 minutes of enzyme assay was reliance according to the highly antioxidantive and antibacterial properties of produced peptides.The proteolysis of shrimp by - product by Alcalase and Pepsin gave highly antioxidantive activity. The isolation , purification and the peptides bioactivity determination was achieved as below : 1 - The peptides of the both protein hydrolysates were isolated by using Ultrafiltration membranes of 5 MWCO KDa. The isolated peptides were examined for their antioxidative activity. It shown , that the peptides wjich was synthesized by the proteolytic activity of Alcalase has ahigher antioxidative action (53.67%) comparing with pepsin peptides (41.19%) at concentration of 100 mg/mL. The results showed that peptides of hydrolysate enzyme Pepsin give inhibitor zoon against bacteria test in inhibition zoon ranged from 10 - 11 mm and determined peptides content of amino acids and showed glysin , threonine ,valine and lysine, which amounted to 9.11% and 8.94% and 7.51% and 9.16%, respectively in peptides of enzyme Alcalase while recorded amino acids serine 9.69% ,theronine 8.76% ,cystin 14.10% and 7.06% lysine ratios highest peptides hydrolysate enzyme Pepsin respectively.Summaryb2 - purification of peptides by gel filtration was showed four peaks of peptides hydrolysate enzyme Alcalase and three peaks for peptides hydrolysate Pepsin enzyme and tested the antioxidation activity of all peaks and inhibitory to bacteria, which recorded that second peak of the Alcalase enzyme antioxidant activity amounted to 63.28% and peptides The first peak of the same enzyme%48.57 the peptides first and the second peak for hydrolysate Pepsin enzyme was% 41.65 and 55.21% also tested the inhibitory effect against some types of bacteria have been to peptides second peak of the Pepsin enzyme hydrolysate towards the bacteria Escherichia coli, Salmonella typhi, Staphylococcus aureus, Bacillus cereus , Pseudomonas aeruginosa inhibition zoon ranged 13 - 9 mm, and determined the minimum inhibitory concentrations (MIC) of these peptides and found that the concentration of 125 and 250 mg / ml values have affected all types of bacteria testing. The cellular toxicity of peptide peakes in analysis of human red blood cells, and has not any toxic effect observed for all concentrations and different periods incubation of peptides peasks.3 - The molecular weight of peptides two of peak enzymes was determined by electrophoresis and cleared two bandes each peak represents two chain peptide molecular weights 3.71 and 4.37 KDa the first peak and the second peak 3.71 and 4.27 KDa of the hydrolysate Alcalase enzyme and chains peptides first peak of hydrolysate Pepsin enzyme 3.71 and 4.16 KDa the second peak, 3.71 and 3.98 KDa, respectively , The study included determined of amino acids and found that it contains all the amino acid varying percentages depending on peptides peakes hydrolysate protein.4 - The stability of antioxidant peptides towards thermal treatment to different degrees thermal ranged between 25 - 100C and change in pH 2 - 11 and treatment of sodium chloride salt ranged between %2 - 8 was studed and found that the antioxidant activity of peptides and reducing power stable at 60C The ability to binding ion ferrous ,hydrogen peroxide , hydroxyl radical scavenging and superoxide anion scavenging they appear stable at thermal 40C and whenctesting the stability of peptides to change the pH was observed that the peptides two peakes hydrolysate enzyme Alcalase stable at pH 8 but decreases when moving away from this value towards the basic or acidic while shwoed peptides two peakes hydrolysate enzyme Pepsin stability at pH 7, and this stability declined when number pH to 11, and when the treatment with salt sodium chloride showed peptides first peak and second enzyme Alcalase and Pepsin stable antioxidant activity at 4% salt concentration for all tests except the oxidation stability of peptides in binding ferrous ion has showed at 6% salt concentration.5 - when the follow of peroxide values for beef patties treated with peptides two peakes enzymes, The results showed there is adecrease in peroxide values for beef patties treated with peptides of the Alcalase enzyme was more cleared compared to the peptides of first peak for the same enzyme and peptides two peakes Pepsin enzyme also got a decrease in the total number of bacteria and total coliform bacteria and psychrophilic bacteria When the treatment second peptides peak of the Pepsin enzyme concentration of 50 and 100 mg / 100 g meat.6 - Sensory evaluation of beef patties showed that the treatment samples with peptides second peak of the Alcalase enzyme recorded the highest degree of sensory evaluation peptides compared with the first peak of the same enzyme and peptides peaks hydrolysate Pepsin enzyme.

انتاج مشتقات الكايتوسان الذائبة بالماء والمستخلصة من قشور الروبيان بتفاعلات ميلارد وتطبيقاتها في بعض الانظمة الغذائية == Production of Water soluble Chitosan derivatives extracted from shrimp shells by Maillard reaction and their application in some food systems

Author name: عاليه جميل علي
Supervisor name: علي حسين عبد الكريم العامري | منير عبود جاسم الطائي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study included the preparation of of chitin from shrimps shells Penaeus semisulcatus and studied of chemical composition of it like moisture,ash and protein wich was 7.80,0.43and3.50%respectively,The yield was 20.0%,then prepared three different types of Chitosan A,B and C from extracted chitin by removing of acetyl group from chitin at different times 4,10 and 20 hour, determination degree of deacetylation for types of Chitosan and standard Chitosanby FTIR 72.30,83.60,98.50 and85.20% respectively as was the viscosity of the three types of Chitosan 137.70,125.21 and74.11 centipoise respectively, selected Chitosan C,which carries ahigher valuedegree of deacetylation 98.50% and studied physico chemical and functional properties,which included (yield ,moisture , ash, protein and solubility) which amounted to16.20, 5.50, 0.20, 1.15 and 98.90%, respectively and reached a molecular weight (17.782) kilo daltone.Capacity of Chitosan to bind fat FBC and water WBC by using three types of oils are (olive oil, corn oil and sunflower oil) were estimated and were givin the highest capacity link with olive oil 665.0%, while the ability to bind Water amounting to 772%, as measured X - ray diffraction (XRD) and use the scanner electron microscope (SEM) to see morphological Chitosan and standard Chitosan Chitosan and the standard Chitosan, while the profile of the thermal gravimetric analysis (TGA) for Chitosan and standard Chitosan show that highest thermal decomposition at 308.56 ,315.92ᵒc respectively .The water soluble Chitosan derivatives were prepared by reaction with five types of reducing sugars by Maillard reactions and the use of three treatments,first treatment at a temperature of 50ᵒc for 1 - 7 days, the second treatment at a temperature of 100ᵒc for 1 - 7 hours and the third treatment at a temperature of 121ᵒc for 1/4 hour by autoclaving, ,and the properties of these derivatives were studied estimated yield, solubility, stability, free amino groups and the change in reducing sugars. highest value of yield gavein by The third - treatment, second treatment, first treatment reached 45.20,43.50,040.0% either solubility reached 1.10 ,0.780, 0.82 g/100 ml and derivatives stability at pH between10.50, 10.0, 9.80 respectively, The largest amount of amino groups and reducing sugars was the first treatment at absorbance 0.590 and0.352 respectively while the second an third treatment amounting to (0.201 and 0.060) respectively for amino groups , and 0.400 and 0.100 respectively for reducing sugars.Properties of antioxidants for prepared Chitosan and its derivatives. The antioxidant activity of Chitosan ranged between17.8 - 73.2%, while the highest activity for the third treatment of derivatives, the second and the first ranged of 76 0.81 - 85.88, 71.30 - 82.70 and 66.01 - 78.50% respectively, The reducing power of chitosan ranged between 0.320 - 0.700, while the highest value of absorbence of reducing power of derivatives to the Third treatment and then the second and finally the first which amounted to 1.290, 1.201 and 0.693 respectively, while the ability of chitosan to chelating ferrous ion and scavenging hydroxyl radical range between27.60 - 72.99%and16.90 - 84.9% respectively.Thehighest percentage to scavenging hydroxyl radical and ability to chelating ferrous ion by derivatives is shown by the third the second and the first treatment reaching 89.98 and 82.8 ,88.80 and 81.90, 84.80 and 76.01 % respectively, the selected third - treatment derivatives (121ᵒc for 1/4 hour) because of its best properties and carried the tests such as analysis of thermal gravimetric (TGA) that was noted three stages to different degrees heat to the disintegration of the derivatives, and the detection of toxicity on human bloodThird treatment compounds identified by GC - MS and the results showed that the derivatives have defferant compounds ,and identified compounds name, molecular formula, structural formula, molecular weight, size and retention time in addition to the profile mass spectrometerIn the practical side the use prepared chitosan in increasing storage time of table eggs, physicochemical tests were carried out for eggs stored at a temperature of 15 ᵒc and for aperiod of six weeks, it included estimation of weight loss (WL) wich amounted to 3.36% for chitosan at the six week as compared with acetic acid and control sample wich 9.01 and 8.96% respectively.Value of yolk Index YI of Chitosan - coated eggs decreased at the sixth week, amounted to 0.35, as compared with acetic acid and control sample wich reached (0.19 and 0.22) respectively, The value of Haugh Unit HU in the sixth week was higher to Chitosan treatment wich amounted to 61.42 compared with acetic acid treatment and control sample wich is24.95 and24.37 respectively.As regards eggs grading EG Chitosan treatment ongradiation A up to fifth week while acetic acid treatment and control sample reached gradiation C , when estimating pH of egg albumin pH high increased has been noted in control and sample treated by acetic acid in compared with chitosan - coated sample wich ranged 9.30, .Chitosan derivatives were also used chitosan as - natural antioxidants in beef products through the follow - up assessment peroxide value at different storage time the highest concentration 0.11g/100g beef meat displays the highest activity to prevent oxidation oil of beef meat wich reached5.11.Asensory evaluation has been carried of product stored on a 4ᵒc for 5 days of recipes sensory color,flavor,juiciness, tenderness and public acceptance using different concentrations.They have shown significant deferences ,but concentration at (0.11g/100g beef meat)was superior over the other concentrations and samples treated with BHT

معايرة معادلات التبخر نتح المرجعي لمنطقتي الفرات الاوسط وجنوب العراق وتاثير جدولة الري والتسميد في بعض خصائص التربة الفيزيائية وكفاءة استعمال المياه لمحصول زهرة الشمس Helianthus annuus L) == Calibration of Reference Evapotranspiration Equations in the Midmost Euphrates Region and Southern Iraq and the effect of irrigation schedule and fertilization on some Soil physical properties and water - use efficiency of sunflower crop (Helianthus annuu

Author name: طالب صبر حريجة العسكري
Supervisor name: داخل راضي نديوي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Theoretical foundations for research included the estimations of reference evapotranspiration (ETo) in the midmost Euphrates region and southern Iraq by applying seven mathematical models as well as CROPWAT 8.0 and CLIMWAT software using two sets of climatic data for periods 1970 - 2002 and 2004 - 2010.The results could be summarized as follow : - 1. The annual mean of ETo calculated by the Penman - Monteith equation (PM) for the meteorological stations of Nasiriya, Amarah, Semawa, Najaf, Diwaniya and Kerballa were (6.00, 6.20, 5.92,5.97,6.13,5.74) mm day - 1 respectively, during the first measurement period, and (6.47, 6.08, 5.86, 5.98, 6.13, 5.65) mm day - 1 respectively, during the second period. Also, the ETo estimated by CROPWAT 8.0 and imported by CLIMWAT software have showed the same trend of PM calculations with overestimate reached (6.10, 5.93) % during the first and secondperiods for CROPWAT 8.0 software, and underestimate for ETo reached 8.16 %during the first period for CLIMWAT software compared to PM calculations.2. The statistical criterions for evaluating the performance of six empiricalequations of the original versions were compared with the Penman - Monteithequation revealed that the variations in their performance with relativelypreference for temperature - based equations in comparison with radiation - basedequations. Blancy - Criddle (1950) equation (BC) has proved superiorperformance in Nasiriya, Semawa, Najaf, Diwaniya and Kerballa stations duringboth periods, also in Amarah station during the second period and declining itsperformance for the benefit of Kharrufa equation (1985) (K) during the firstperiod of measurement.3. The calibration process of the six empirical equations including : Hargreaves - Samani (1985) (HS), Makkink (1957) (M) , Priestley - Taylor (1972)(PT),andHargreaves (1975) (H) in addition to BC and K equations were made bysubstituting recalibrated constant values for the original constant values afterthese constant had modified to fit PM calculations and it used a set of statisticalcriterions to verify the validity of calibration and its ability to reduce theestimates errors compared to the original formulas. As the indices ofconcordance (D), confidence (C), correlation (r) and regression (R2) togetherwith the Nash - Sutcliffe Efficiency(NSE), standard deviation ratio (RSR). meanabsolute error (MAE), root mean square error (RMSE), relative error (RE)indicators and the ratio between both average estimations of ETo (r) are used toassess the validation of calibration and optimization which revealed thatconfidence became high in modified equations with reducing the estimation errorof ETo due to approach the values of these criterions from optimal value, then,they had improved efficiency of the six equations to simulate the values of EToare more accuracy than original formulae.4. The proposed new constants for the estimation of the ETo with : BC was 1.37for the time periods from January to April and 1.39 for May to December insteadof the original constant 1 : 41, HS was for the time periods from Octoberto March and for April to September instead of the original constantb0.0023, K was for June - July and for remained months instead of theoriginal constant 0.34, M are for the time periods from November to Mayand for June to October instead of the original constant 0.61, the equationsPT and H were 1.96 and 0 0.0165 for the period of December - May and 2.61and 0.0178 for the period from June to November instead of the originalconstants of 1.26 and 0.0135 respectively and equationsThe side applied to the study has included the implementation of the fieldexperiment for the cultivation of sunflower crop through the loop spring 2013 inprivate farm lies according to the geographical positioning system (GPS) withinthe coordinates (606,366.3437642), (606320, 3437729), (606353, 3437749),(606400, 3437660), is located in Batah town, Thi Qar province, at a distance of 17km west of the city of Nasiriyah, up 5 meters from the sea surface. The mainobjective of experiment included the following : i. Measuring ETo for the study through the crop growing seasonii. To determine the crop coefficient (Kc) and the actual water consumption(ETa) of sunflower crop in the Batah areaiii. . To asses effect of irrigation scheduling and fertilizer application as well astheir combinations on the growth indicators, nutrient uptake, dry matter,crop yields, the crop water use efficiency and water productivity.iv. Evaluating impact of irrigation scheduling and fertilizer application besidestheir combinations on some of soil physical properties at the end season.A field experiment was carried out using factorial experiment in a randomizedcomplete block design (RCBD) Layout with three replications in two factors, nineirrigation Scheduling treatments were assigned to the main plots and four fertilizertreatments were assigned to the subplots. Sunflower seeds (flame) were planted onMarch 23 with plant density 61538 plant ha - 1; the harvest operation was conductedon 18 / July / 2013.The irrigation Scheduling was achieved based on replenish root zone to theborders of field capacity when it reach the percentage of management allowabledepletion (MAD) identified about 55% of total available water (TAW), taking intoaccount the diversity of the effective depth of the root zone according to the stagesof growth, . The experiment involved Irrigation scheduling of the sunflower basedby calculating ETa using Kc which adjusted in situ multiplying by ETo thatestimated by PM equation (S1) ,original BC equation (S2) and adjusted K equation(S3) using meteorological data for real time that collected from agrometeorologicalstation nearby the field. PM equation (S6) using historicalmeteorological data and from pan evaporation with applied (FAO 56, 1998) tocalculate pan coefficient (S4). Whereas, irrigation Scheduling based on soil waterbalance according to daily measurements of soil moisture and adjusted daily onthe basis of changes in moisture content (soil water balance) (S5), fixed irrigationinterval and variable irrigation depth (S7), different irrigation interval and variableirrigation depth (S8) and soil water balance for bare soil (S9). Also the studyincluded four fertilization treatments were used ( F : mineral fertilizer, C : Compost,M : mineral fertilizer + Compost , N : non fertilization). The results showed thefollow : c1. The maximum value of ETo during season growth of sunflower crops accordingto field measurements has increased significantly when using evaporation pan,which gave the highest average rate of 8.81 mm day - 1 in comparison with PMequation that recorded the lowest value of ETo with mean equal to 8.00 mm day - 1,while BC and K gave 8.66 and 8.14 mm day - 1 respectively.2. The converged seasonal mean of Kc value for sunflower crops during growthseason were 0.864, 0.862, 0.863, 0.861, 0.862, 0.862, 0.861, 0.862 for S1 - S8treatments respectively, while the Kc varied in different stages of growth, thehigher value of Kc was 1.213 observed during the flowering stage, followed bytwo phases of vegetative growth and maturity with mean 0.796, 0.494respectively, whereas the lowest values (0.350) was recorded in the initial stage3. The ETa values were variable with various irrigation Scheduling treatments,the treatments (S1 - S8) gave mean ( 876.86, 938.98, 885.57, 943.40, 898.47,949.05, 835.61, 839.76) mm Season - 1 respectively, the mean values of ETa aredistributed as 52.28% during flowering stage, 23.38% through maturity, 18.97%during vegetative growth stage and 5.36% in the initial stage4. The Irrigation scheduling of the sunflower based on S5 treatment caused toincrement of water consumption by transpiration during flowering stage to reach83% from total ETa depth, whereas The Irrigation scheduling based on S7treatment caused to increment of water consumption by evaporation during thesame stage to reach 29% from total ETa depth.5. The results demonstrated that all Irrigation scheduling have been showed astrong relationship with water balance measurement (S5), by R2 is greater than0.91 and r greater than 0.95, also, the results showed there was a preference to therevised K equation to calculate ETa of sunflower crops in the region according toindicators of regression model each of the R2, slope and intercept which amountedto 0.9395, 0.9875, - 0.0144 respectively.6. The overall mean of net irrigation depth was 874.6 mm season - 1 ranged from941 mm season - 1 for S4 treatment to 800 mm season - 1 for S7 . the addition ofwater was 14 applications for S1 - S4 treatments and 15, 13, 12 applications for S5,S8, S7 respectively.7. The lowest nitrogen concentration in the leaves of Sunflower crops duringflowering stage related to decrease water supply at S7 (27.69 g kg - 1) , then, itincreased to maximum value ( 31.48 g kg - 1) when irrigation Scheduling based onS1 followed by S3, S5, S2, S4 treatments,. As respect with maturation stages theS5 treatment gave maximum mean (26.81 g kg - 1) was significantly superiorcompared to all treatments except S1 and it gave increment in nitrogenconcentration equal to 22% in comparison with S8 which recorded lowest nitrogenconcentration (21.94 g kg - 1). The results showed the F treatment was significantlysuperior followed by M treatment which gave average 34.43, 33.42 g kg - 1 duringflowering stage and 28.69, 26.48 g kg - 1 during maturation stage respectively.8. Increasing irrigation depth due to an application S4 and S6 treatments andIncreasing the irrigation frequency for S5 treatment led to significant increment inthe bulk density for 0 - 15 cm depth with mean 1.393, 1.384, 1.386 M m - 3respectively, the bulk density decreased steadily with decreasing of irrigationddepth and irrigation interval until reach their lowest level (1.350 M m - 3) at S7treatment. The bulk density was significantly decreasing when C and M treatmentswere applied with means of equal to (1.362, 1.364) M m - 3 respectively, atmagnitude 1% higher than their initial values. Also the results showed theirrigation scheduling treatments and fertilizer application as well as theircombinations failed to exhibit any significant influence on bulk density for depths15 - 30 cm and 30 - 45 cm.9. The results showed a significant increase in the soil penetration resistance forsoil with high watery supply (S4 and S6), while that status is significantlydecreased under treatment of low watery supply (S7, S8 and S1), the soils withmedium watery supply causing the pattern moderate between the two, also, thisepithet is significantly reduced when you add compost with an average of 708.09kPa, while there was a significant increase resulted from control treatment andaddition of chemical fertilizer alone, the proportion of 31.81 and 18.41%sequentially compared with the addition of compost.10. The results showed the possibility basic infiltration rate for most treatmentsunder the category (moderately slow) according to Landor (1991), by the mean ofbasic infiltration rate was 1. cm h - 1 ranged between 0. to 3. cm h - 1, whereasbasic infiltration rate for S4N and S9 decreased to 0.6 , 0.51 cm h - 1 respectively,thus it can be classified into (slow) while it increased greater than 2.0 cm h - 1 forS1C, S1M, S2M, S5C, S7F, S7M,S7C, S8M treatments which refer to moderateinfiltration rate according to the same classification . C and M fertilization led toimprove soil physical properties, which in turn positively affected the infiltrationrate with increment in magnitude (5 , 56) % respectively, compared to Ftreatment.11. Treatment of F gave a significant superior in most morphologicalcharacteristics and yield components, the efficiency of F treatment for productionwas 37.45 % followed by M and C treatments were achieved 24.66, 19.24 %respectively, compared to N treatment.12. Treatment F achieved highest yield of grains 1945.80 kg ha - 1, an increasing was24.32% in comparison with N treatment which gave lowest yield of grains reached1367.24 kg ha - 1, also there were a significant increase in crop water use efficiencyand water productivity are associated with application of F and M fertilization13. The productivity characteristics of sunflower were significantly affected byirrigation Scheduling treatments, The highest seed yield 1893.8 kg ha - 1and thehighest dry matter 5246.2 kg ha - 1were obtained under S5 treatment, while the S7treatment recorded lowest values of these indicators (1512.5 and 3765.4) kg ha - 1 respectively,14. The application of Irrigation scheduling based on S5, S1 and S3 treatments achieved a significant increase in crop water use efficiency and water productivity, the mean of first parameter was (0.219, 0.216, 0.216) kg m - 3 respectively, and themean of the second parameter was (0.228, 0.220, 0.219) kg m - 3 respectively.

تاثير حقن بيض التفقيس بفيتامين A وE وD3 وخليطهم في بعض الصفات الانتاجية والفسلجية والسلوكية لطائر السمان الياباني (Coturnix japonica) == Effect Of In Ovo Injection of Vitamin A , E , D3 and AD3E on some Productive , Physiological and Behavioral Traits of Japanese Quail )Coturnix japonica)

Author name: صلاح مهدي كاطع سلمان
Supervisor name: طارق فرج شوكت | قصي موسى جعفر
General topic: Agricultural sciences
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in the Animal Production field which belong to the Technical College - AL - Massaiab - AL - Furat AL - Awsat University which included two experiments the first was during 4/3/2014 until 22/3/2014, the second was during 22/3/2014 until 10/8/2014 and the to investigate the effect of injection of japanese quail eggs A,E,D3 and AD3E Vitamins on some productive , reproduction, physiological , behavioral and hematological traits .A total number of 720 eggs were used in the experiment ,those eggs were randomly distributed into six treatments , 120 egg in each treatment . The eggs were injected before the first day of incubation in the air sac by vitamins. Treatments were arranged as fallow : T1 : Negative control treatment .T2 : Positive control treatment , it included injection of 50 microliter of corn oil for each egg.T3 : It included injection of 50 microliter per egg by vitamin A (100 IU/egg).T4 : It included injection of 50 microliter per egg by vitamin E (15 IU/egg).T5 : : It included injection of 50 microliter per egg by vitamin D3 (100 IU/egg).T6 : : It included injection of 50 microliter per egg with the blend of AD3E vitamins by the same concentration per egg.The most important results recorded in the first experiment were high significant increased (P≤0.05) of hatchability of fertile egg and post chick weight but not effect significant on embryo mortalities during embryogenesis .The second experiment included rearing of the post hatched chicks of the first experiment in wooden dataries , chicks were all randomly allocated inSummarybto three replicates for each treatment (17 chick / replicate) to study productive , reproductive , physiological, behavioral , histological , biochemical and hematological traits for Japanese quail.Results illustrated the followings : 1 - A significant increase (P≤0.05) of productive traits in treatments of injection (body weight , weight gain , feed utilization , feed conversion ratio ) and find significant increase egg production , egg weights and mass as well as shell characteristics (egg shell weigh and thickness ) in Treatments (T5 , T6 ) comparisons with the negative and positive control treatments .2 - A significant effect (P≤0.05) on early maturation for males and females in treatments of vitamins injection , more over there were a significant increase of their body weights during maturation and early behavioral jumping with a significant surpass of cloaca area in comparison with males in control treatments.3 - A significant increase (P≤0.05) of relative weights of testes in males of all ages in injection treatments with a significant increase of seminal tubules diameters and thickness of germinal layer of testes during 28 and 47 days in comparison with that of the negative and positive controls.4 - A significant increase (P≤0.05) of relative weights of ovaries with an increase of the primary follicle diameter in females of injection treatments during 28 , 47 and 69 days in comparison with that of the control .5 - A significant increase (P≤0.05) of relative weight of fabricia gland and spleen for both sexes of birds in injection treatments , more over the interaction between vitamins injection and sex was significant in all ages.6 - A significant increase (P≤0.05) of PCV in injection treatment , moreover the PCV was increased in females at 28 and 47 days, the interaction between vitamins injection and sex was significant in all ages .7 - No significant increase (P≤0.05) was noticed in Hb of injection treatments at 28 but there were a significant increase (P<0.05) in 47 days in vitamins injection treatments and there was a significant increase (P≤0.05) of Hb in 69 days in the sixth treatment in comparison with the control treatments, and it was numerical on other treatments ( 3rd, 4th and 5th) there was no interaction between vitamins injection and sex at 28 days but the interaction was significant in at 47 and 69 days .8 - There were no significant increase (P≤0.05) of RBC in injection treatments at 28 days but it was significant at 47 days in the third and sixth treatments , at 69 days there was a significant increase of RBC in injection treatment as compared with the control treatments , the interaction between injection of vitamins with sex was not significant at 28 days but it was significant at 69 days .9 - There were no significant effect (P≤0.05) in WBC in vitamins injection at 28 , 47 , 69 days as compared with the control treatments moreover there was no significant interaction between injection treatment and sex.10 - There were significant increase (P≤0.05) in LH , FSH and testosterone levels in serum of vitamins injection treatment for both sexes , the interaction between treatment sex was significant in all ages .11 - There were a significant surpass (P≤0.05) of total protein level in injection treatments at all ages as compared with the control treatments and the interaction between treatments and sex was significant at all ages . glucose and cholesterol level were not affected according to the treatment but females were significant surpasses on males at all ages . The interaction between treatment and sex was not significant at all ages12 - There were a significant surpass (P≤0.05) of Ca and P in injection treatment at all ages as compared with control treatments , the interaction between treatment and sex for Ca and P level was significant at all ages .13 - The study revealed no significant improvement in concentration Ca , P and ash in tibia bone , weight and length of thigh for both sexes in injection treatment at all ages .

تاثير انظمة الاضاءة والتقنين الغذائي الزمني في بعض الصفات الانتاجية والفسلجية والتناسلية لطائر السمان الياباني (Coturnix japonica) == The Effect of Lighting Systems and Feed Restriction timing on Some Productive, Physiological and Reproductive Traits of Japanese Quail (Coturnix japonica)

Author name: صباح كاظم مرزوق الحمود
Supervisor name: ریاض كاظم موسى | ربیعة جدوع عباس
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تصميم وتصنيع وتقييم الاداء الميكانيكي لالة زراعية من خلال وضع السماد العضوي في خنادق تحت سطح التربة وتاثيرها في بعض خصائصها ونمو وحاصل الذرة الصفراء Zea mays L == Designing , constructing and evaluating the mechanical performance for agricultural machine laying the manure under the soil surface and its effect on some of soil properties and growth and yield of corn ( Zea mays L.

Author name: صادق جبار محسن
Supervisor name: شاكر حنتوش عداي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: ditch opener and manure laying machine was designed and manufactured in the agricultural machines and equipment dept., Agriculture college, Basrah university in 2015. This machine was evaluated to determine its mechanical performance and its ability in laying manure in ditches under the soil surface as well as the effect of the added manure on the soil physical and chemical properties, corn crop growth and production parameters.The machine consists of a frame, manure tank, chemical fertilizers tank, subsoiler for soil disturbance down to 80 cm, two boards for soil digging out to open the ditch , two boards to return the soil to ditch to bury the manure , manure and chemical fertilizers feeding mechanisms and blades for manure mixing with the soil. Also its provide with two pipes to transfer the chemical fertilizers to the soil and a petrol engine to operate the two feeding mechanism.The machine disturbed the soil by the subsoiler and digging different ditches of different depths by two boards . In additional to that it can lay the manure at different levels in the opened ditches using the feeding mechanism and thereafter buried the manure in the soil . The manure can be laid under the soil as separate layer or mixed with soil using the mixing blades.Three experiments were conducted to evaluate the machine performance. These experiments are : (1) - Laboratory experiments were conducted to study the effect of the machine forward speeds (0.32, 0.46 and 0.63 m sec - 1), manure feeding mechanisms rotating speeds (360, 440, 560, 710 and 880 rpm) and two manure feeding openings areas (0.024 and 0.048 m2) as well as the interaction of the above parameters on the amount of the manure per hectare (ton ha - 1). These results were analyzed using CRD and the statistical program (Genstat). Mean results were compared using least significant difference (RLSD) at 0.05 level.The results showed the manure amount per hectare significantly increased as the rotating speeds of the feeding mechanism increased,Bthe forward speeds decreased and the feeding opening areas increased. The highest value was recorded for forward speed of 0.32 m sec - 1 , rotating speed of 880 rpm and feeding open area of 0.048 m2. These means are 64.17, 62.06 and 54.06 ton ha - 1 respectively. while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and the feeding open area of 0.024 m2 gave the lower values which they are 26.91, 30.04 and 33.75 ton ha - 1 respectively. Also, the forward speed of 0.32 m sec - 1 , the rotating speed of 880 rpm and the feeding area of 0.048 m2 recorded the highest amount of manure per hectare (110.00 ton) while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and manure feeding area of 0.024 m2 recorded the lowest value of manure per hectare (4.00 ton).(2) - A field experiment was conducted in silty loam soil in the agriculture college field at Garmit Ali location to study the effect of the implement operation depths (10, 20, 30, 40 and 50 cm), the angle between the soil digging out boards (45⁰ and 60⁰) two soil types namely cultivated soil before one year and uncultivated soil and their interaction on the implement mechanical performance parameters . These parameters are the draft force, soil disturbed area and the energy utilization efficiency (EUE) as well as the draft force requirement of the implement units (subsoiler, ditch opening boards, soil returning boards and soil mixing blades). The results were analyzed using RCBD. The analyzing was carried out by the statistical program (Genstat). Mean results were compared using RLSD at probability level of 5% , and showed the followings : (2 - 1) - The draft force, the disturbed area and the energy utilization efficiency increased significantly when the operating depth increased from 10 to 50 cm. The amount of the increase is 26.66 kN , 0.4111 m2 and 7.67 m3 mJ - 1 respectively.(2 - 2) - The angle between the opening boards of 60⁰ significantly surpassed angle of 45⁰ in increasing the draft force, the disturbed area and EUE. The percentage of increase is 12.55 , 21.93 and 10.85 % respectively.C(2 - 3) - The draft force decreased while the disturbed area and EUE increased by 16.30, 14.91 and 20.72 % in the disturbed soil compared with undisturbed soil respectively.(2 - 4) - The draft force requirement of the subsoiler was higher than that for the other implement parts. The share of the draft force requirement of the subsoiler was 60 % out of the total draft force requirement of the implement while it was 26, 9 and 5 % for the ditch opening boards, the soil returning boards and soil mixing blades respectively.(2 - 5) - The draft force requirement of all implement units increased significantly with implement operating depths and the angle between the ditch opening boards and it was higher in the uncultivated soil compared with the cultivated soil. The draft force requirement of the soil returning boards and the soil mixing blades in both soil types was not significantly different.(3) - Another field experiment was conducted in the field of the agriculture college at Garmit Ali location in the season of 2015 in a cultivated soil of silty loam texture. The aims of the experiment were to study the effect of the added manure level (0, 20 and 40 ton ha - 1), the depths of added manure (10, 20 and 30 cm) and the methods of the addition (subsoil layer and mixing with soil) and their interaction on the soil properties and corn crop (Zea mays L.) growth parameters and yield at the end of the growth season.The soil was plowed and harrowed (soil clods breaking down). The manure was added thereafter by the implement in the soil according to the studied treatments. The corn seeds were sown in rows at 1/7/2015. The drip irrigation method was used to irrigate plants until the end of the experiment 10/10/2015. The other agricultural operations were conducted according to methods used in the around area.Soil and plants samples were collected for analysis and the corn seeds yield was calculated at the end of the growth season. The measurements included soil bulk density, soil moisture content, soilDsalinity, soil pH, the dry weight of the shoot, N, P, K uptake in shoot and the total seeds production.The split - split plot in RCBD design was used to analyze the means using Genstst program. Mean results were compared using RLSD at probability level of 5 %, and showed the followings : (3 - 1) - The manure level of 40 ton ha - 1 surpassed the other two levels (0 and 20 ton ha - 1) in giving higher values of soil moisture content, higher uptake of N, P, K, a greater amount of dry weight and total yield while it gave lower soil bulk density, soil salinity and soil pH.(3 - 2) - Increasing the depth of added manure from 10 to 30 cm with the mixing method lead to significant increase in the soil moisture content, uptake of N, P, K, the dry weight and the total yield production. Whereas the soil bulk density, soil salinity significantly reduced while the soil pH was not significantly affected.(3 - 3) - Mixing of 40 ton manure ha - 1 at depth of 30 cm gave the highest crop growth and yield production which they are 13.94 and 6.75 ton ha - 1, respectively.

عزل وتشخيص بكتريا Bacillus cereus واستعمالها في انتاج متعدد هيدروكسي البيوترات == Isolation and Identification of Bacillus cereus Bacteria and Using in Polyhydroxybutyrate Productio

Author name: شيماء ذياب جدوع السهلاني
Supervisor name: امال كاظم غضبان الاسدي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Forty three local bacterial isolates were obtained after heating treatment for different sources collected from many places in Basra government. Included : Vegetables, Fruit, Pastry (cakes), Legumes, Soils, Sand, Animal manure, the papyrus plant, milk, water liquefaction and sewage water. Primary screening for the isolates by Sudan black B dye only. 22 isolates were chosen with strong staining, and the identification of all the isolates revealed were belong to genus Bacillus by studying microscopic and biochemical tests. They were : 4 isolates Bacillus cereus, 2 isolates Bacillus firmus, 2 isolates Bacillus lichenformis , 3 isolates Bacillus megaterium, 4 isolates Bacillus mycoides , 3 isolates Bacillus pumilus and 4 isolates Bacillus subtilis.Secondary screening of these isolates was Polyhydroxybutyrate (PHB) produced, Bacillus cereus B5 given 2.4 g / L the highest production of PHB. A PCR technique was used for 16S rRNA test and detecting the gene of PHB production in Bacillus cereus B5.The highest PHB production from Bacillus cereus B5 was 6.2 g / L, biomass 8.4 g / L and yield 73.8% the by using optimum conditions : incubation temperature 35̊ C, for 48 hours aerobically by using shaking incubator for 150 RPM/ min, 2% inoculum volume, the pH was adjusted to 7, and the production media where contained 1% glucose as carbon source and peptone as a nitrogen source. 3% have dated juice was the best substitute for glucose as carbon source, it gave 7.11 g PHB / L and the yield 79 %.Analysis with FT - IR was showed, that PHB produced from date juice media had a peak at 1723 cm - 1 this means it belong to an esterpolymers group. And GC - MS showed 12 compounds produce from analyzing PHB as short chains of fatty acids.The properties of PHB produced from date juice media were : The degradation temperature was 312̊ C, the percentage of crystallization was 60%, molecular weight was 423.674 KDa., permeability of water vapor was 56.2 ×10 - 8 g / Pa.s.m2, tensile strength was 24.9 MPa, the blend 40% polyethyleneglycol as plasticized with PHB increased the percentage of elongation to 8.7%. PHB is non - toxic and without hemolysis on human blood.Studying biodegradation of the plastic films of PHB by using bacterial isolates were E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus in petri dish showed that all isolates can degrade PHB, biodegradation in soil and at soil surface was 100% after 28 days.PHB packages increased the shelf life of strawberries and grapes after storage for 15 days, compared with polyethylene packages, and It was reduced the numbers of microorganisms in butter, the percentage of free fatty acids and peroxide value compared with butter with polyethylene after 15 days

تاثير اضافة النتروجين في امتصاص NوPوK وتوزيعها في اجزاء النبات ونمو وحاصل ثلاثة اصناف من الحنطة Triticum aestivum L. == EFFECT OF NITROGEN APPLCATION ON N, P, K UPTAKE AND DISTRIBUTION WHIHIN PLANT PARTS, GROWTH AND YIELD OF THREE WHEAT CULTIVARS Triticum aestivum L.

Author name: سندس عبد الكريم محمد العبد الله
Supervisor name: عبد المهدي صالح حسين الانصاري | وليد عبد الرضا السباهي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A field experiment was conducted at Al - Daire site 40 km north of center Basrah Governorate .The study was conducted to investigate the effects of different nitrogen fertilizer levels on dry matter accumulation, (N, P, K) uptake, distribution in different parts of three cultivars of wheat plants and their yield during two seasons (2011 - 2012 & 2012 - 2013).The experiment was split - plots in R.C.B.D design with three replicates, Nitrogen rates (0, 60,120 and 240Kg ha - 1 occupied the main plots, while (IPA - 99, Abu - Graib and Latifia) Cultivars Occupied the sub - plots. Samples of wheat plants were collected at 10 growth stages : seedling stage, tillering (main shoot and 1 tiller), tillering (main shoot and 4 tillers), stem elongation, booting, 50% of spike emergence, 50% flowering, milk stage, dough stage and maturity. Each plant sample was separated into different plant parts : blades, sheathes, stems, flag leaves, spikes and seeds. These parts were dried at 70°C and dry weight of each part was recorded and sub sampled for chemical analysis of N , P , and K. Growth parameters (No. of day till 50% flowering , No. of day from 50% flowering to Maturity , flag leaf area , plant height, spike length and No of tillers m - 2) , yield components (No. of spike m - 2, No. of grain in spike and weight of 1000 grain ),grain yield, biological yield, harvest Index, grain protein percentage and nitrogen use efficiency were also studied.Results showed that dry matter accumulation in plants of all cultivars did not differ at early growth period, While after the growth stage of 50% flowering plants dry weight of Cv.IPA - 99 was higher than other cultivars, This difference in dry weight continue till maturity stage at which total dry weight were 14473.58, 13373.96 and 12743.59 Kg ha - 1 for IPA - 99, Abu - Graib - 3 and Latifia respectively.Dry matter yield were not affected by N levels during the early growth period ,then after dry weight of plants increased as N rates increased ,total dry weight were10752.61, 12737.49 and 15347.10 Kg ha - 1for 0, 60 , 120 Kg Nha - 1 respectively, with no differences between 120 and 240 Kg Nha - 1 levels.Dry matter of plant parts differed among cultivars, with IPA - 99 having the highest results. Increasing nitrogen rates increased weight of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.IIResults also showed reduces in dry weight of different plant parts(except grains)after reaching a maximum weigh at dough stage for Spikes, and 50% flowering for the other parts. The highest decreased was in blades and lowest in sheathes. The application of N fertilizer decreased translocation of dry matter from plant parts to grains. Data showed that no differences in nutrient uptake were observed for cultivars till ending of tillering stage, but after this stage IPA - 99 cultivar showed highest uptake till the end of the season. Nitrogen uptake was157.68,136.72,124.51 Kgha - 1 ,P uptake 19.70,16.93,15,67 Kg ha - 1, K uptake136.30,121.10,112,60 Kg ha - 1 for IPA - 99,Abu - Graib - 3 and Latifia, respectively.Increasing N rate increased nutrient uptake of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.uptake of N, P, and K for all cultivars and nitrogen rates declined as the season progressed, this decline differed among nutrients and plant parts.Grain contained 59.20, 79.00 and 7.45% of total nutrient N, P and K uptake, whereas vegetative parts contained 40.80, 21.00 and 92.55% of total nutrient uptake, respectively.Cultivar IPA - 99 resulted in highest grain yield among studied cultivars. Increasing N rate from 0 to 120 Kg N ha - 1 only increased grain yield, the rate 240 KgNha - 1 did not affect yield significantly as compared with that of 120 Kg N ha - 1.Results also showed a significant interaction between Cultivars and N rates. The highest grain yield obtained at treatment IPA - 99×240 Kg N ha - 1with no significant differences than IPA - 99×120 KgNha - 1.Results indicated that highest nitrogen use efficiency (66.23%) was found in plant of IPA - 99 cultivar, whereas, the lowest efficiency (47.37%) was found in plant of Abu - Graib - 3 cultivar. At all cultivars increasing N rates to 240 KgNha - 1 decreased nitrogen use efficiency.Therefore it can be concluded that 120 Kg Nha - 1 can be recommended as the best rate for N to all cultivars under similar growing conditions

تقييم المضافات الغذائية في بعض المنتجات الغذائية المحلية ودراسة تاثيراتها الكيميائية والفسلجية والنسيجية في الفئران المختبرية == Evaluation of food additives in some local food products and study their chemical, physiological and histological effects in laboratory mice

Author name: رنا داود سلمان الكامل
Supervisor name: علي احمد ساهي | قاسم فوزي عبد الكريم
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Due to the progressive expansion in the use of chemical additives in food processing associated with absence of quality control and loss of proper application of the international standards of food additives, making human beings exposed to high risks from ingestion of these foods which might be so mild in cases like simple poisoning and more complex like cancers, the present study was performed, which includes four axes : The First Axis : 1 - A questionnaire study of food additives : the results of which indicated that 55.42% of consumers eat packed food continuously, and most of them(61.44%) have no interest in reading the labels on the outside cover of the packaging , despite the fact that 49.39% of them consume it daily.Whereas 51.80% of consumers had no specific knowledge or familiarity about food additives, 54.14% of them do not possess any knowledge about their side effects and 77.10% of them did not know the symbols listed out.2 - A survey of some food products that used sodium benzoate as a preservative in their manufacture. The results indicated that 163 types of food samples contain sodium benzoate, including different products such as soft drinks, canned juices, sherbet, jams, tomato sauce, pickles, ketchup and ambah.3 - A survey of some food products in which sugar enters in their composition, These include 231 products which mentioned the word sugar or artificial sugar within labels on the outside without mentioning its kind .These products include : imported ice cream, jams, candies, soft drinks and diet drinks, cake, sherbet , fruit preserved in sugar solution and biscuits. 4 - A questionnaire study of the market sales of locally made pickles. The results indicated that : 71.79% of consumers frequently ate pickles in large quantities despite the fact that 48.71% of them does not have any information about sodium benzoate preservatives added to some of its , whereas 64.10% of them have no information about its side effects that result from the ingestion of these products beyond the allowable limits. 5 - A questionnaire study for the market sales of locally made ice cream.The results showed that : 77.77% of consumers ingested ice cream in large quantities despite the fact that 63.88% of them did not have any information on the sweetener saccharin added to some of its. While 62.50% of consumers possess no information about the side effects resulting from consumption of products that contain artificial sweeteners used in food processing especially when there is more than the allowablelimit.

اثر اضافة الكبريت الزراعي واليوريا والسوبر فوسفات في نشاط انزيمي اليوريز والفوسفاتيز وحاصل الذرة البيضاء Sorghum bicolor L. == Effect of Agricultural Sulfur , Urea and TSP application on the activity Of Urease phosphatase and yield of (Sorghum bicolor L. )

Author name: رشاد عادل عمران الزبيدي
Supervisor name: هيفاء جاسم حسين التميمي | عبد الزهرة طه ظاهر
General topic: Agricultural sciences
Specific topic: Soil and Water - Fertility
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

استخدام بعض المرشحات في خفض الملوحة والبورون في مياه شط العرب وابار جنوب البصرة لاستعمالها في ري نبات الطماطة Lycoperscion esculentum Mill == Using of Some Filters in Dercreasing Water Salinity and Boron Toxicity of Satt Al - Arab River and Basrah Southern Wells and Reuse of Tomato Plant (Lycoperscion esculentum Mill) Irrigation

Author name: حنان عبد الوهاب سعيد الحكيم
Supervisor name: هيفاء جاسم حسين التميمي | نجلة جبر محمد الاميري
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Thirteen water sample of Shatt Al - Arab river and its tributaries were collected starting with Madania at (north of Basrah ending with Al - Fao south of Basrah) according to the following areas (Medania , Gurna , Sharesh, Deyar/ near paper plan, Hartha (near thermal electric station), Garmat Ali (Basrah university), Tenoma/Sahehia , near teaching hospital, Abul - Khaseeb /Hamdan, Abul - Khaseebl Abu - Floose, Sebha, Al - Dora, and Al - Fao. Fifteen well's water sample were collected from Al - Zubair , Al - Berjesia and Safwan during Dec.2014. Chemical and physical properties of water samples were conducted (ECiw), soluble ions (calcium, mangnesium, sodium, potassium, chloride, sulphate, carbonate, bicarbonate, boron, and nitrate), total dissolved solids (TDS), and total hardness. After water sample properties determination, they were classified to three classes according to electrical conductivity (1 - 4, 4 - 8, and 8 - 12) dSm - 1 , and three classes of boron concentrations (< 0.7, 0.7 - 3.0, and > 3.0) mg L - 1 . For the purpore of study of studies filter efficiency, two type of filter were used, mineral filter (sand, charcoal, porcelin, and cement klint dust) and organic filters (rice hust ash, barley straw, sawdust, plam leaf, and poultry rnanure). Two experiments were conducted, one of them laboratory experiment in soil science and water resources laboratories /College of Agriculture /University of Basrah) other was biological experiment in wooden canopy to achieve the objectives of the study.Nine type of mineral and organic types of filters were selected for studying their efficiencies in decreasing water salinity and boron toxicity of Shatt Al - Arab river and Wells, after passing water through them. Chemical and physical properties of water were determined after passing water through filters and water quality was calculated. A number ofBcriteria and international standards were used for evaluation water quality after and before passing through filters.For studying the efficiency of water treatment by the most efficient filters according to laboratory experiment results were rice husk ash, cement klint dust, sand, and control treatment. Biological experiment was conducted by using plastic pots with capacity of20 kg soil according to factorial experiment with three replicates for each treatment with 24 empirical units (2×3×3) (soil * No. of filters * replicates) besides control treatment and six classes of water to be 144 empirical units. Two soils were used one of them from Agricultural field of Agricultural Research Station, and the other from Garmat Ali field during agricultural season 2014 - 2015. Mineral fertilizers were used with rates, 320 Kg N ha - 1as urea (46 % N) with two doses, phosohate fertilizer was applied at rate 90 kg P ha - 1 as concentrated supper phosphate (20.21 % P), potassium at rate of 120 kg K ha - 1 as potassium sulfate (43 % K) one dose at planting. Peatmose fertilizer was applied at rate 2% for all treatment mixing with soils. For getting erough water for irrigation, plastic coloumns wereduplicated in their dimentions for studied filters. Shatt Al - Arab and wells water were passed through most efficient filter besides sand filter separately with equal quantities. Water was collected after one minute to conform the properties of laboratory experiment.Tomato plants (Lycopersiconesculintium M.)were planted on 22/9/2014 and irrigated with water treated with filters besides control treatment at field capacity (20 % for Berjesia Research Station soil) and (30 % for Garmat Ali soil). Leaching requirement was calculated for each treatment. After 176 days, plants were harvested with space 2 cm from soil surface avoiding contamination. Tomato plants were fractionated into three parts, roots, shoots, and fruits. Dry matter of roots and shoot wasBCcalculated. Boron concentrion of (roots, shoots and fruits) was determined. Fruits yied of tomato was calculated. Electrical conductivity and available boron of residual soils in pots were determined after tomato plants growth Period.Results obtained could be summarized as : 1. Adoption of rice husk ash and cemenentklint dust in treatment of Shatt Al - Arab and wells water because of their high efficiency in decreasing boron concentration and water salinity and reuse for tomato plant irrigation and Plants irrigated with water treated with rice husk ash were surpassed over other plants in dry matter and yield, and gave lowest boron concentration in tomato plant tissues for all parts roots, shoots, and fruits. Same treatment gave the lowest concentration residual boron in soil and lowest value of soil salinity as compared with water treated with cementklint dust filter and sand filter besides control treatment.2. loamy sand soil was surpassed over clay loam soil in dry matter and yield of tomato and lowest concentration of boron in their tissues, while clay loam soil was surpassed over loam sand soil in residual boron concentration and soil salinity.3. Results indicated that there were significant differences between type of filters and soil texture in dry matter, yild , boron concentration in roots , shoots, and fruits of tomato plants. Residual boron concentration and soil salinity.4. Results indicated that there was no boron contamination in both studied soils treated with water of Shatt Al - Arab and wells treated with two filters (rice husk ash and cementklint dust)

تاثير بعض معاملات السيطرة الطبيعية قبل الحصاد وبعده في تحسين الصفات النوعية والقابلية الخزنية لثمار نخيل التمر Phoenix dactylifera L. صنفي البرحي والبريم == EFFECT some pre and post - harvest natural control treatments on improving the qualitative characteristics and storage ability of date palm fruits (Phoenix dactylifera L. ) cvs. Barhi and Bream

Author name: حمزة عباس حمزة عباس
Supervisor name: عباس مهدي جاسم | ضياء احمد طعين
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Research were divided into two experiments, field and storage , field experiment was conducted in private orchard at Abu - Alkhasib south of Basrah . Date palm fruits( Phoenix dactylifera L.) cvs. Barhi and Bream were used during the growing seasons 2014 and 2015. The aim of the study was to investigate the effect of spraying chitosan at the concentrations of 0% , 1% , 2% on yield and some physical , chemical and physiological characteristics of fruits. The experiment included 9 trees for both cvs. Complete Randomized Block Design was used with three replicates . The results were analyzed by the analysis of variance of factorial experiment and mean values were compared using the Revised Least Significant Difference Test at 0.05 probability level. The results of study showed significant effect of spraying chitosan at the concentrations of 2% on the most of the studied parameters (fresh weight , size of fruit , yield , water content, total acidity ,tannin and pectin) in addition to delay the ripening and respiration rate of fruits for both cvs. Untreated fruits retain the highest percentage of total soluble solids , total sugars , reducing sugars and total protein. Invertase and cellulase activities decreased in fruits treated with chitosan as compared with untreated fruits which recorded the highest activity of invertase and cellulase for both cvs.Storage experiment was conducted in Al - Moussawi dates factory situated at Abu - Al - Khaseb District , Basrah Governorate to study the effect of some pre and post harvest natural control treatments in improving the qualitative characteristics and storage ability of date palm fruits cvs. Barhi and Bream. Fruits that treated with chitosan at the concentrations of (0, 1, 2) % were harvested in the early morning and carried to Al - Moussawi dates factory. Then every one of the three parts divided into five groups ,the first was treated with ultraviolet light (UV) at the concentration of 1KGY for periods of (0, 5, 10) minutes ,while the second group were treated with (1 - MCP) at the concentration of ( 0,0.5,1) ppm for 24 hour at 0 ºc .third was treated with ozone (O3) at the concentration of 5 ppm for the periods of (0, 0.5, 1) hour ,while the fourth group were soaked in chitosan at the concentrations of ( 0,1,2) % , the fifth were without treatment .Fruits were packed in plastic bags.Both of the two parts were stored at two storage temperatures (room temperature 25±2ºc and freezing temperature - 10±2ºc for three weeks and six months respectively . Storage characteristics were studied before andBduring storage and at the end of storage period . the results can be summarized as follow : 1 - spraying chitosan at the concentrations of 2% in the field reduced the percentage of deteriorated fruit , ripening process, TSS , total and reducing sugars and increased the percentage of water content, sucrose and total acidity in addition to elongation the desirable color of fruits and keeping good flavor and taste for Barhi and Bream fruits until the end of storage period at - 10±2ºc and 25±2ºc for the both of growing seasons 2014 and 2015 .2 - Treating with ultraviolet light (UV) for 5 minutes reduced percentage of decay for Barhi ,whereas fuel west percentage of Bream fruits decay was in fruits treated wish ozone for 1hour for both growing seasons .3 - Treating with (1 - MCP) at the concentration of (1) ppm reduced the progress of ripening and total sugars and increased water content, sucrose and total acidity in addition to elongation the desirable color of fruits and keeping good flavor and taste for Barhi and Bream fruits up to the end of storage period at - 10±2ºc for the both of growing seasons .4 - The percentage of decay of fruits , ripening progress, loss in weight , TSS , total and reducing sugars increased whereas the percentage of water contents, sucrose and acidity decreased with increment of storage period for both cultivars and seasons at 25±2ºc and - 10±2ºc.5 - The interaction between field treatments and storage treatments had significant effect on percentage of fruits decay. Fruits sprayed with chitosan at the concentrations of 2% in the field and treated with ultraviolet light (UV) for the periods of 10 minutes had the lowest percentage of fruits decay for both growing seasons .6 - The interaction between spraying chitosan and storage period significantly effected on studied parameters . Fruits sprayed with 2% chitosan in the field were superior in keeping water content and delaying ripening process but they had the lowest TSS , total and reducing sugar at the end of storage period at 25±2ºc and - 10±2ºc compared with untreated fruit for both cultivars and seasons .7 - The interaction between storage treatments and storage period significantly effected on studied parameters. Fruits treated with (1 - MCP) at the concentration of (1) ppm were the best in keeping water content and delaying ripening process but they had the lowest TSS , total and reducing sugar at the end of storage period at 25±2ºc and - 10±2ºc compared with untreated fruit for both cultivars and seasons .C8 - The interaction among field treatments, storage treatments and storage period had significant effect on percentage of fruits decay. Fruits sprayed with chitosan at the concentrations of 2% in the field and treated with ultraviolet light (UV) for the periods of 10 minutes had the lowest percentage of fruits decay , at the end of storage period at - 10±2ºc for both cultivars and seasons .9 - Gel electrophoresis for protein showed that in Barhi and Bream fruits treated with pre and post harvest natural control treatments and stored at - 10±2ºc , the process of gene expression has occurred , since there was a number of bands , which differed both in number as well in their position on the gel , depending on treatments and cultivar . There was very clear differences between treatments in the number , position and the timing of the appearance of the protein bands for both cvs. during which Bream fruits treated with chitosan recorded the highest number of bands ( seven bands).

التوصيف الجزيئي والتعبير الجيني لجين بروتينات الصدمة (الحرارية 00 hsp70) في السائل المنوي لثيران الهولشتاين العراقية والكباش العرابية == Molecular Characterization and Gene Expression of Heat Shock Protein 70 (hsp70) Gene in the Semen of Iraqi Holstein Bulls and Arabi Rams

Author name: حسن نعمة حبيب السنيسل
Supervisor name: عماد فلاح حسن | بسام ياسين خضير
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted at the Animal Farm of College of Agriculture, University of Basrah, (longitude 47.7433690, latitude 30.5627250 north of Basrah), and the fields of Artificial Insemination Center in Abu Ghraib, the General Company for Livestock Services (longitude 44.1922070, latitude 33.3095550 northwest of Baghdad), during the winter season for the months November, December 2015 and January 2016, and the summer season for the months April, May and June, 2016. To achieve all the study requirements, the laboratories work at the Laboratory physiology, Molecular Genetics Laboratory, College of Agriculture, University of Basrah; the Central Research Unit, College of Veterinary Medicine, University of Basrah; the Criminal Laboratory Department of Investigation, Criminal Evidence Directorate, Basrah Governorate Police; as well as laboratories of the Abu Ghraib, Artificial Insemination Center; in addition to the First BASE Laboratories, Laboratory in Selangor Malaysia, were used. The study included two experiments : The first experiment : This experiment was designed to detect the genetic polymorphism of hsp70 gene in the semen of Bulls, their relationship to certain physical and chemical semen characteristics, the level of gene expression in the season winter and summer, and the evaluation of the bulls by finding the breeding value of certain traits in the fresh and frozen semen of the Iraqi Holstein Bulls, back to the Artificial Insemination Center / Abu Ghraib / Baghdad, 30 bulls were used in this experiment.Second Experience : This experiment was designed to detect the genetic polymorphism of hsp70 genes in the semen of Rams, their relationship with some physical and chemical semen characteristics, the level of gene expression in the season winter and summer, and the evaluation of the rams by finding the breeding value of some traits in the fresh and frozen semen of local Arabi rams, back to the Agricultural Research Station / College of Agriculture / University of Basrah, 20 Rams were used in this experiment.Results of the study : I. The first experiment : 1) After DNA extracted, the quantity and purity of the DNA were determined by the Nano drop device, the purity ratio was 1.8. A Polymerase Chain Reaction (PCR) carried out, the amplification product was approximately 1926bp. Nucleotide Sequence analysis was done to detect polymorphism and compared with the genotypes of the hsp70 gene in the Gene Bank. There are three Genotypes of hsp70 gene were obtained in bulls : : The Genotype A, this genotype is the closest to heat shock protein hsp70 gene in the Holstein bulls in Gene Bank. This polymorphism was exposed to be genetic mutations at position No. 6, the Nucleotide (C < G), No. 174 (C < G), No. 282 (A< G) and position No. 1339 (T < C). All mutations are silent that did not encodingto any new amino acid, (16 Bulls).B : The Genotype B, the nucleotides were substituted at different positions, position No.114 (G < A), No. 174 (C < G), No. 282 (A < G), No. 1339 (T < C),No.1451 (C < A), No.1590 (A < G), No. 1695 (C < T) and position No. 1719 (G<T), these genetic mutations are all silent except the mutation at the position No.1451 is a missense mutation, encoding for new amino acid, (6 Bulls).C : The Genotype C , the nucleotides were substituted at different positions, position No. 114 (G < A), No. 174 (C < G), No. 282 (A < G) No.1339 (T < C),No. 1590 (A <G), No. 1695 (C <T) and position No. 1719 (G <T), all mutationsare silent, ( 8 bulls).2) The results of the Multiple Sequence Alignment (MSA) of hsp70 gene in the bulls showed that the convergence ratio of A, B and C were 99.73%, 99.51% and 99.56%, respectively, with the same gene in the Holstein bulls recorded in Gene Bank. The MSA results also showed that the B genotype gave the lowest ratio to the studied genotypes, followed by the C genotype and the A genotype, which showed the highest approximation with the genotypes studied in the Gene Bank for the Holstein bulls, sheep, goats, pigs and humans.3) Genotype B, has shown significantly higher (P < 0.05) than the C genotype and A genotype in all physical semen characteristics studied, it gave the highest rate of mass and individual motility, the highest percentage of live sperm and thelowest rate of abnormalities, both in fresh and frozen semen in a period of onemonth and two months freezing and in both seasons of the study.4) Genotype B showed Significantly higher (P < 0.05) towards the C and A genotypes in all chemical semen characteristics studied, It gave the highest percentage of the Acrosome integrities and gave the lowest level of concentration of enzymes ALT, AST and ALP in the frozen semen and freezing periods in a month and two months and in both seasons of the study.5 ) After RNA extraction, synthesise of cDNA and carry out Quantitative Realtime PCR (qRT - PCR) analysis, genotype B showed Significantly higher (P < 0.05) than genotype C and A in the level of gene expression of heat shock protein hsp70 gene in the summer season.) The bulls belong to genotype B were superior in the valuation on the basis of the breeding value of motility, live sperm and the abnormalities in the fresh and frozen semen for a month and two months followed by the C than A.II. Second Experience : 1) After DNA extracted, the quantity and purity of the DNA were determined by the Nano drop device, the purity ratio was 1.8. A PCR carried out and the amplification product was in approximately 1926bp, bands were visualized by gel electrophoresis. Nucleotide sequence analysis was done to detect polymorphism compared to that of the hsp70 gene in the Gene Bank. There are two genotypes ofhsp70 gene were obtained in rams : A : The A genotype, showed no match with other nucleotides sequence of other group neither heat shock protein hsp70 gene in Gene Bank, the nucleotide in position 1308, the nucleotide T was substituted by C (T < C), a silent genetic mutation that did not encoding a new amino acid, (10 rams).B : The B genotype, in comparison with the A genotype , the Nucleotide C was substituted with G (C <G), a missense genetic mutation that resulted from change the amino acid encoding at the site 514, a genetic mutation not previously recorded in rams except in inese sheep. In addition, nucleotide G was substituted by nucleotide A (G < A) in the position 1524, a silent genetic mutation (10 rams).2) The results of the (MSA) of hsp70 gene in the rams showed that the ratio of Aand B genotypes were 99.89% and 99.84% respectively with the same gene inrams in the Gene Bank. The MSA of the genotype B gave the lowest ratio to thesame gene in sheep, Holstein bulls, goat, pig and human3) The genotype B Significantly higher (P <0.05) than the genotype A in allPhysical semen characteristics studied, it showed the highest rate of mass andindividual motility and the highest percentage of live sperm and the lowest rate ofabnormalities, in fresh and frozen semen in a period of a month and two monthsfreezing and in both seasons of the study.4) The genotype B Significantly higher (P <0.05) than the genotype A inall chemical semen characteristics, it showed the highest percentage of theAcrosome integrities, and showed the lowest level of concentration of enzymesALT, AST and ALP in the frozen semen and freezing periods of month and twomonths and in both seasons of the study.

تنقية وتوصيف انزيم اللايبيز المستخلص من بذور فول الصويا المنبتة واستخدامه كمحسن للخبز == PURIFICATION AND CHARACTERIZATION OF LIPASE EXTRACTED FROM SOY BEAN SEEDS AND USED IT AS IMPROVER IN BAKING

Author name: بتول محمود محمد الانصاري
Supervisor name: علي احمد ساهي | ضياء فالح الفكيكي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة استخلاص وتنقية انزيم اللايبيز من سبعة انواع من البذور الزيتية قبل وبعد الانبات وهي(فول الصويواالسلجم وزهرة الشمس والعصفر والدخن والذرة الصفراء والخروع). اظهرت نتائج تقدير الفعالية النوعية ان افضل نوع للبذور الزيتية هو بذور فول الصويا اذ اعطت اعلى فعالية نوعية للانزيم في البذور قبل الانبات وبعد الانبات 3.58 و11.20 وحدة / ملغم بروتين على التوالي مقارنة مع بقية الانواع الاخرى. استخلص اللايبيز من بذور فول الصويا قبل وبعد الانبات باستعمال تسعة محاليل استخلاص ووجد ان افضل محلول للاستخلاص هو محلول Tris - HCl تركيز 0.1 مولاري الحاوي على 0.01 مولاري كلوريد الكالسيوم و0.001 مولاري اثلين داي امين تترا استك اسد و0.001 مولاري كلوريد المغنسيوم وبدالة حامضية 8 , اذ انه اعطى اعلى فعالية نوعية. درس التركيب الكيميائي لبذور فول الصويا قبل الانبات وخلال مدة الانبات المختلفة اذ لوحظ ارتفاع نسبة البروتين في البذور المنبتة لتصل ذروتها عند نهاية اليوم الخامس من الانبات والذي بلغ 41.60% مقارنة بنسبة البروتين قبل الانبات 35.57 %. اما نسبة الدهن والكاربوهيدرات والرماد فقد انخفضت في البذور خلال مدد الانبات اذ بلغت 17.75 و30.18 و5.97 % على التوالي مقارنة بالبذور غير المنبتة 20.74 و32.5 و6.26 % على التوالي. تم متابعة تطور اللايبيز في مدة الانبات لبذور فول الصويا, اذ امتلك اعلى فعالية في اليوم الخامس من الانبات حيث بلغت 38.00 وحدة/ مل . اجريت خــــــــــــطوة الترسيب التدريجي للانزيم باستعــــــــمال نسب اشباع متدرجــــــــــــــــة من كبريتات الامونيوم 30 - 90 %, اذ لوحظ حدوث ارتفاع واضح بشكل تدريجي للفعالية النوعية للانزيم في الراسب الناتج لغاية نسبة اشباع 80 % وقد اعطت هذه الخطوة فعالية نوعية مقدارها 324.44 وحدة / ملغم بروتين وحصيلة انزيمية بلغت 29.20 % بعدد مرات تنقية مقدارها 10.78 مرة. اما خطوة التنقية بالترشيح الهلامي باستعمال عمود الترشيح الهلامي Sephadex G - 100فقد بلغت الفعالية النوعية 366.66 وحدة/ ملغم بروتين وبحصيلة انزيمية 17.83% وبعدد مرات تنقية 12.18 مرة. وبينت نتائج تحديد نقاوة اللايبيز ظهور حزمة بروتينية واحدة عند الترحيل الكهربائي في هلام متعدد الاكريلامايد بغياب العوامل الماسخة, في حين بلغ الوزن الجزيئي 41.687 كيلو دالتون بطريقة الترحيل الكهربائي في هلام متعدد الاكريلامايد بوجود العوامل الماسخة . وجد ان الدالة الحامضية المثلى لفعالية الانزيم كانت 8 , في حين تراوحت الدالة الحامضية المثلى لثبات الانزيم بين 7 - 9 اذ احتفظ الانزيم بـ 90 % من فعاليته. بينما لوحظ ان درجة الحرارة المثلى للفعالية الانزيمية للانزيم المنقى هي 40 م وان الانزيم يفقد 67 % من فعاليته على 90 م . اظهرت نتائج تاثير الايونات المعدنية والعوامل المختزلة والكلابية على فعالية اللايبيز ان لايونات الزئبقيك والخارصين والحديدوز بتركيزي 1 و5 ملي مولاري تاثير تثبيطي في فعالية الانزيم ،اذ بلغت الفعالية المتبقية 63.2 و68.9 و66.1 % على التوالي, في حين احتفظ الانزيم بكامل فعاليته عند تركيز 5 ملي مولاري بوجود ايونات الكالسيوم والبوتاسيوم. كمااظهرت العوامل الكلابية والمختزلة ان للـ EDTA دورا تنشيطيا عند حضنه مع اللايبيز بتركيز 1 و5 ملي مولاري. ولوحظ ان اللايبيز لا ينتمي الى مجموعة الانزيمات الفلزية (metalloenzyme). اما اليوريا فكان لها دور تثبيطي على فعالية الانزيم عند تركيز1 و5 ملي مولاري, في حين بلغت الفعالية المتبقية للانزيم 86.2 % عند حضنه مع 1% Mercaptoethanol 2 - , بينما كانت الفعالية المتبقية للانزيم عند حضنه مع Hydrogen peroxide وSodium hypochlorid 74.7 و75.8 % على التوالي بتركيز1 % .ولوحظ ان لمواد الشد السطحي تاثيروااضحا على فعالية الانزيم عند تركيز 1 و5 %, اذ احتفظ الانزيم بكامل فعاليته عند حضنه مع Triton X - 100 وTween 20عند تركيز 1% . في حين تاثرت فعالية اللايبيز باضافة المنظفات التجارية, ولكنها اظهرت ثباتية عالية في وجود المنظف Sar حيث بلغت الفعالية المتبقة 99.1 %. بينت النتائج تباين النسبة المئوية للاحماض الامينية الموجودة في اللايبيز اذ لوحظ ان اعلى تركيز كان لليوسين Leu بلغ 16.4 % بينما اقل تركيز كان للاسبارتك ASP 1.5 % في حين تفاوتت الاحماض الامينية الاخرى في نسبها المئوية. اظهرت نتائج فحص الفارينوغراف وجود فروق معنوية بين قيم متوسطات نسب امتصاص الماء للمعاملات واظهر تركيز 0.004 ملغم لايبيز / 100 غم طحين و0.75 % محسن Ovalette اعلى نسبة امتصاص للماء 61.7 و59.9 % على التوالي , في حين ازدادت فترة الاستقرار معنويا 9.6 و9.9 دقيقة عند تركيز 0.002 و0.004 ملغم لايبيز/ 100 غم طحين و13.3 و11.6 دقيقة عند تركيز0.50 و0.75 % محسن. اما قيم متوسطات فترة النضج للعجين فقد ازدادت مع زيادة تركيز الانزيم 0.002 و0.004 ملغم لايبيز/ 100 غم طحين معنويا اذ كانت 7.2 دقيقة وارتفعت فترة نضج العجين عند جميع تراكيز المحسن 6.8 و8.5 و6.2 دقيقة على التوالي مقارنة مع العجين الخالي من اية اضافة. وجد من العلامات النهائية للتقويم الحسي ان افضل معاملة كانت عند اضافة اللايبيز بتركيز 0.002 ملغم لايبيز/ 100 غم طحين و0.5 % Ovalette وكان المجموع النهائي للمعاملات 95 و94 % على التوالي. لوحظ من نتائج فحوصات تاثير اللايبيز على تجلد الخبز ان قيم كل من حجم الراسب في عالق اللب المائي وقوة التشرب ورطوبة اللب انخفضت تدريجيا مع زيادة مدة حفظ الخبز, اما نسبة الرطوبة في القشرة فقد ازدادت مع تقدم مدة الحفظ. وكان تاثير اضافة اللايبيز والمحسن Ovalette ايجابيا على الصفات النوعية ( الحجم ولون ونضارة اللب) والخزنية (تاخر تجلد اللب) للخبز المختبري | The study included extraction and purification of lipase enzyme from seven kinds of oil seeds before and after germination, those were (soy bean ,rapeseed, sunflower, safflower, pearl millet, yellow corn and castor bean seeds). The results of spcific activity estimation showed that , the best kind of oil seeds are soy bean seeds, they gave the highest specific activity of enzyme before and after germination 3.58 and 11.20 unit/ mg protein respectively comparing with other varieties. Lipase was extraction from soy bean seeds before and after germination using nine extraction solutions the best extractant solution was Tris - HCl 0.10 M containing 0.01M calcium chloride and 0.001mM ethylene di amin tetra acetic acid and 0.001 mM magnesium chloride pH 8. Lipase improvement was monitored through the germination period of soy bean seeds, it reached the highest activity of lipase after five days of germination ,which was 38.00 unit / ml .The chemical composition of soy bean seeds studied before germination period and through different germination period , higher protein percentage was noticed in the germination seeds where it reached its peak at the end of five days of germination 41.60 % compared with protein percentage before germination 35.57 %. For the percentage of fat, charbohydrates and ash they decreased in the seed through germination periods, down to 17.75 %, 30.18 % and 5.97% respectively compared with non - germinated 20.74 % and 32.50 and 6.26% respectively. The gradually precipitated by addition of ammonium sulphate to final saturation of 30 - 90%. Agradual obvious higher specific activity of the enzyme in the precipitate resulted ,till saturation level 80% , this step gave aspecific activity of 324.40 unit/ mg protein and an enzyme yield of 29.20% ,purification folds 10.79 time. While purification step of gel filteration using Sephadex G - 100 where the specific activity 366.66 Unit / mg protein , enzyme yield of 17.83 % and purification fold 12.18. Results for determination of lipase purity showed an appearance of a single protein band by poly acrylamide gel electrophoresis with out of denaturizing agent . The molecular weight of enzyme was 41.687 kilo dalton by poly acrylamide gel electrophoresis with denaturizing agent . The optimum pH for enzymtic activity of the purified enzyme were 8 while the optimum pH stability profile of the enzyme was between 7 - 9, and the enzyme kept 90% of its activity, while notes that the optimum temperature for the enzymatic activity of the purified enzyme was 40 C ͦ, and that enzyme loses 67% of its activity at 90 C ͦ. The resulte of effectness of metal ions and reducing and chelating agent was showed that ions of mercury and zinc ions and ferrous at concentrations of ( 1 and 5 ) m M have an inhibition effect on enzyme activity, as the remaining activity reached the values 63.2 .68.9 and 66.1% respectively , while the enzyme kept its total activity at concentration of 5 m M in the presence of calcium and potassium ions. The chelating and reducing agents showed that EDTA has an active rule when the enzyme incubated with 1 and 5 mM of lipase. And it was noticed that lipase dosn΄t belong to the metalloenzyme group. For urea , it has an active rule on the enzyme activity at 1 and 5 M ,where remaining activity for the enzyme reached 86.2% when incubated with 1% 2 - mercaptoethanol while it was 74.7 and 75.8 % respectivly when incubate with 1% of Hydrogen peroxide and Sodium hydrochloride respectively. Concentration ,for enzyme kept its total activity when incubated with Triton X - 100 and Tween 20 at concentration 1%. While lipase activity did n΄t effected by adding commercial detergents, it also showed a high stability in present of Sar detergent where as residual activity reached 99.1 % . Results cleared the contrast of amino acids existin lipase because it is noticed that the highest concentration reached by leucine was 16.4% where as lowest concentration was that reached by aspartic acid 1.5% ,the other amino acids differed in their percentage . The results of pharinograph test showed that there were significant differences between the mean of water absorption the ratios showed that the concentration of 0.004 mg / 100 g flour of enzyme and 0.75% improver give highest absorption of water with significant of moral 61.7 and 59.9% respectively, while the stability period increased to 9.6 and 9.9 minutes at a concentration of 0.002 to 0.004 mg / 100 g flour of enzyme and 13.3 and 11.6 minutes at a concentration of 0.50 and 0.75% improver with significant differences. The average values of maturity period of the dough increased with increasing concentration of the enzyme 0.002 and 0.004 mg / 100 g flour with significant difference as it was 7.2 minutes and increased the maturity period of the dough at all improver concentrations of 6.8, 8.5 and 6.2, respectively, compared with the dough without any addition. Found from the final marks of sensory evaluation ,it is found that the best process was when add lipase at 0.002 mg / 100 g flour concentration and 0.5 % Ovallete ,the final marks were 95 and 94 % respectively. Its has been noticed that the values of volume of sediment in the crumb aquous suspended ,absorption power and crumb moisture, all reduced gradually with the increasing of loaf storage time. While the moisture content of crust was increased The effect of adding enzyme and improver Ovallete was positive regarding the specific characteristics of loaf such as ( volume ,color and softness of crumb) for loaf.

تاثير التقنين الغذائي الكي واضافة المعزز الحيوي وحامض البروبيونك في العلائق على الاداء الانتاجي والفسلجي لفروج اللحم == Effect of Quantitative feed restriction with Addition Probiotic and Propionic Acid on Productive Performance and Some Physiological Characters of Broiler Chicks

Author name: الفريد سولاقه كرومي حنا
Supervisor name: رياض كاظم موسى | عبد الله عبد المنعم محمد
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted at poultry farm, Animal Resources Department, College of Agriculture University of Basrah from 4/4/2010 to 15/5/2010 for the first experiment and from 8/112010 to 19/12/2010 for the second experiment. The aim of this study was to investigate the effect of early (8 - 21) and late (21 - 34) day of feed restriction by partial 20% with adding IRAQI probiotic at level 0.3% for (first experiment) and propionic acid at level 0.3% for the (second experiment) in productive performance and some physiological of blood and small intestine. One day of 270 unsexed chicks of Ross 308 strain were used in the study. The chicks were randomly distributed into 6 treatments with three replicates. Each replicate contain 15 chicks. Results of first experiment indicated that : 1 - There was a significant (p<0.05) increase in control treatment with IRAQI probiotic on live body weight and body weight gain. While, there was a significant (p<0.05) decrease in the late feed restriction treatment with, or not adding probiotic.2 - There was a significant (p<0.05) increase for the control treatment with, or not adding probiotic in feed consumption.3 - There was a significant (p<0.05) improvement in feed conversion ratio of early and late feed restriction with, or not adding probiotic.4 - Late feed restriction and early feed restriction with probiotic cause significant (p<0.05) decrease in mortality comparing with control treatment.]b[5 - There was a significant (p<0.05) decrease in abdominal fat for the treatments early and late feed restriction with, or not addition probiotic as compared with the control treatment.6 - There was significant (p<0.05) reduction in Intestine PH for the treatment adding probiotic, but there was no differences between the treatments in Ceca PH.7 - There was a significant (p<0.05) decrease in total protein, albumin, cholesterol and glucose in blood serum for the chicken with early feed restriction (not adding probiotic), and there was significant (p<0.05) increase GOT and GPT levels in the same treatment at the age of 21 days, Addition of IRAQI prpbiotic showed a significant (p<0.05) reduction of serum cholesterol, GOT and GPT as compared with control treatment.8 - There was a significant (p<0.05) decrease in cholesterol, GOT and GPT by the addition IRAQI probiotic compared with control treatment at 42 days.Results of second experiment indicated that : 1 - There was a significant (p<0.05) decrease in the treatments (late feed restriction, early and late feed restriction with, or not adding propionic acid) on live body weight and body weight gain.2 - There was significant (p<0.05) decrease in feed consumption for the treatment early and late feed restriction with, or not addition propionic acid. 3 - There was significant (p<0.05) decrease in feed conversion for the treatment early and late feed restriction with addition propionic acid compared with the other treatments.4 - Late, early feed restriction with or not addition propionic acid cause significant (p<0.05) decrease in mortality compared with control treatment, and there was significant (p<0.05) reduction in production index for the treatment early and late feed restriction with addition propionic acid as compared with the other treatments.5 - There was a significant (p<0.05) decrease in abdominal fat for the treatments early and late feed restriction with, or not addition propionic acid compared with the control treatments.6 - There was a significant (p<0.05) decrease in total protein, globulin, cholesterol and glucose in blood serum for early feed restriction with, or not adding propionic acid, but there was significant (p<0.05) increase at the level of GOT and GPT in the same treatment compared with the other treatments at the age of 21 days.7 - Serum cholesterol showed significant (p<0.05) reduction in the late feed restriction with, or not adding propionic acid compared with the other treatments at the age of 42 days.

التركيب النوعي والموسمي لهائمات بعض الاسماك وعلاقاتها الغذائية في الجزء الشمالي من شط العرب == The Spatial and Seasonal composition of Some Ichthyoplankton and trophic relationships in north part of Shatt Al - Arab River

Author name: منى طه خضير العكيلي
Supervisor name: عبد الرزاق محمود محمد | سمية محمد احمد
General topic: Agricultural sciences
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: درس التركيب النوعي والموسمي لبيوض الاسماك ويرقاتها في الجزء الشمالي من شط العرب، باعتماد الادلة البيئية للتنوع مع التركيز على العلاقات الغذائية بين انواع يرقات الاسماك وامكانية التداخل الغذائي فيما بينها. جمعت العينات للفترة من كانون الثاني 2008 والى حزيران 2009 ، اذ تم اختيار خمس محطات لجمع العينات. اعتمدت وسيلتان لجمع عينات بيوض الاسماك ويرقاتهواهي شباك الهائمات حجم فتحات الشباك 100 - 300 مايكرون واستخدام مربع خشبي (50 سم Χ 50 سم) لجمع النباتات المائية التي قد تتواجد عليها بيوض الاسماك ويرقاتها. تراوحـت درجة حرارة الماء في منطقة الدراسة بين 11.2 ºم خلال كانون الثاني و33 ºم خلال اب والملوحة بين 1.81 جزء بالالف خلال كانون الاول و3.3 جزء بالالف خلال اب، ونفاذية الضوء بين 20 سم خلال تموز و150 سم خلال كانون الثاني. جمعت 360 عينة هائمات سمكية بواقع 72 عينة لكل محطة وتضمنت العينات 7220 بيضة اسماك و7360 يرقة اسماك. صنفت البيوض الى ثلاث عوائل سمكية متمثلة بعائلة الشبوطيات Cyprinidae التي شملت بيوض الكارب الشائع Cyprinus carpio والكارب الذهبي Carassuis auratus وعائلة البياح Mugilidae التي ضمت نوعا״ واحدا״ وهو الخشني Liza abu وعائلة البطريخ Cyprinodontidae التي ضمت نوعا״ واحدا״ Aphanius sp. . سادت بيوض الخشني في محطة كرمة علي (1 ) والشلهة (3 ) والسندباد (4 ) وبيوض الكارب الذهبي والشائع في محطتين النجيبية (2 ) والجباسي(5). تواجدت بيوض الكارب الذهبي والشائع والخشني في اربعة اشهر (شباط - ايار) والبطريخ من نيسان الى تموز. سجل اذار ظهور اكبر عدد لبيوض الخشني والكارب الذهبي والشائع، بينما سجل اكبر ظهور ليرقات البطريخ في نيسان. صنفت اليرقات الى خمس عوائل، شملت عائلة الشبوطيات Cyprinidae يرقات الكارب الشائع C. carpio والكارب الذهبي C. auratus والسمنان الطويل A. mossulensis ، وعائلة البياح Mugilidae الخشني L. abu وعائلة البطريخ Cyprinodontidae ، اذ صنفت يرقاتها الى مستوى النوع Aphanius sp. . تمثلت عائلة الصابوغيات Clupeidae بيرقات الصبور Tenualosa ilisha وعائلة نصفية المنقار Hemiramphidae تمثلت بيرقات القمبرور Hemiramphus sp.. سادت يرقات الخشني على الانواع الاخرى في المحطات الخمسة، وجاءت يرقات الكارب الذهبي في المرتبة الثانية في المحطات 1 و2 و4، واحتلت يرقات البطريخ المرتبة الثانية في محطة 3 ويرقات الصبور في المرتبة الثانية لمحطة 5. ظهرت يرقات الصبور في ستة اشهر خلال السنة (اذار ونيسان وحزيران الى ايلول) وسجل اب ظهور اكبر عدد ليرقاتها في محطة 4، وظهرت يرقات البطريخ في خمسة اشهر (نيسان الى اب) وسجل نيسان ظهور اكبر عدد ليرقاتها في محطة 3. ظهرت يرقات الخشني والكارب الذهبي والشائع في اربعة اشهر (شباط الى ايار) وسجل اذار ظهور اكبر عدد ليرقاتهم عند المحطة 3 للخشني والكارب الذهبي وعند المحطة 1 للكارب الشائع. ظهرت يرقات القمبرور في اب عند المحطة 5. سجلت اعلى وفرة لبيوض الاسماك ويرقاتها تلك التي جمعت من على النباتات المائية مقارنة بتلك التي جمعت باستخدام شباك الهائمات، اذ دلت نتائج التحليل الاحصائي وجود فروقات معنوية بين المحطة 1 والمحطات 2 و3 و4 و5 في وفرة بيوض الاسماك ويرقاتها التي جمعت بواسطة شباك الهائمات، وبين المحطة 3 والمحطات 1 و2 و5 في وفرة يرقات الاسماك التي جمعت من على النباتات المائية. تم حساب ادلة التنوع ليرقات الاسماك. تراوح دليل التنوع H بين صفر - 1.068، ودليل التكافؤ J بين صفر - 0.884، وقيم دليل الغنى D بين صفر - 0.739. اظهر التحليل العنقودي ان اعلى تشابه في عدد انواع اليرقات بين المحطتين الثانية والثالثة، الثانية والرابعة، الثالثة والرابعة حسب دليل جاكارد. ووجد ان اعلى تشابه في العدد الكلي لليرقات كان بين المحطتين الاولى والخامسة حسب دليل شونر. اعتمدت في تحليل غذاء يرقات الاسماك طريقتان النقاط Points وتكرار الـتواجد Frequency of occurrence. لم تسجل يرقات بمعد فارغة اثناء الدراسة وهناك ارتفاع في شدة ونشاط التغذية. بين تحليل محتويات القناة الهضمية ان اليرقات غـير متخصصة فـي تغذيتها، شمل محتـوى القـناة الهضمية الهائمات الحيوانـية والنباتـية ( دايتومات، طحالب، فتات عضوي، مجذافية الاقدام بمراحل حياتها المختلفة، براغيث الماء وبيوض النواعم). تتغذى يرقات الكارب الذهبي والشائع على الدايتومات ويرقات السمنان الطويل والبطريخ والصبور على مجذافية الاقدام. لوحظ ان اعـلى قيـمة لـدليل التـنوع الغذائي ليرقات الخشني بلغ 1.731 وادناها ليرقات الكارب الشائع اذ بـلغ 0.762 . تباينت يرقات الاسماك في شدة التداخل الغذائي Diet overlap فيما بينها اعتمادا على طبيعة التغذية، وبلغت عدد التداخلات بين اليرقات تسع تداخلات معنوية، وتراوحت قيم التداخل المعنوي بين 67.10% و91.73%. بلغ اعلى تداخل غذائي (91.73 %) بين يرقات الكارب الشائع والذهبي. كان تاثير التداخل الغذائي قويا״ جدا״ بين يرقات السمنان الطويل والبطريخ (101.22) ولم يظهر تاثير للتداخل الغذائي بين يرقات الكارب الذهبي والشائع ويرقات كل من الصبور والبطريخ والسمنان الطويل. بينت الدراسة اهمية الجزء الشمالي من شط العرب ونهر كرمة علي كمنطقة تكاثر وحضانة لبعض الاسماك. | The present work has taken in consideration investigating the spatial and seasonal composition of fish eggs and larvae in north part of Shatt Al - Arab River during the period from January 2008 till June 2009. Five sampling stations were chosen. Ecological diversity indices were adopted, with a focus on the dietary relations among species of fish larvae and the possibility of dietary overlap within larvae. Two sampling methods were adopted to collect the samples, plankton nets with mesh sizes 100 - 300µm and a wooden quadrate to collect aquatic plants that may exist upon fish eggs and larvae. Water temperature of the studied area ranged form 11.2ºC in January to 33 ºC in August. Salinity ranged form 1.81‰ in December to 3.3‰ in August. Light penetration ranged from 20 cm in July to 150 cm in January. A total of 360 samples were collected, 72 samples from each station. These samples included 7220 fish eggs belonged to three families and 7360 fish larvae belonged to five fish families. The eggs were identified Cyprinidae included Cyprinus carpio and Carassuis auratus, Mugilidae (Liza abu) and Cyprinodontidae (Aphanius sp.). The eggs of L. abu were prevailed at stations 1,3 and 4, and the eggs of C. carpio and C. auratus at stations 2 and 5. The eggs of C. carpio , C. auratus and L. abu were presented in four months ( February - May), and Aphanius sp. from April to July with peak during April. The eggs of L. abu , C. carpio and C. auratus were most abundant during March. Larvae were classified into five families included. Cyprinidae, which included the larvae of C. carpio , C. auratus and A. mossulensis, whereas, Mugilidae included a single species ( L. abu), and Cyprinodontidae, where the larvae were classified to the species level Aphanius sp., Clupeidae represented by Tenualosa ilisha, and Hemiramphidae by Hemiramphus sp.. Larvae of L. abu prevailed on other species in the five stations, and the larvae of C. carpio came in second place in the stations 1,2 and 3. Larvae of Aphanius sp. Occupied second place in station 3 and the larvae of T. ilisha in the second place in station 5. Larvae of T. ilisha appeared during six months (March, April and June to August ) with peak during August in station 4, while the larvae of Aphanius sp occurred at five months (April to August), with peak during April in station 3. The larvae of L. abu , C. carpio and C. auratus occurred in four months ( March to May) , with peak in total number during March at station 3, for L. abu and C. auratus and at station 1 for C. carpio. Larvae of Hemiramphus sp. appeared in August at station 5. The abundance of fish eggs and larvae collected from aquatic plants more than provided by using plankton net, as indicated by the results of statistical analysis there were significant differences between station 1 and stations 2,3,4 and 5 in abundance of fish eggs and larvae which were collected by plankton net, and between station 3 and stations 1, 2 and 5 in the abundance of fish larvae collected from aquatic plant. The diversity index (H') for fish larvae ranged between 0 - 1.068, while the richness index (D) ranged between 0 - 0.884 and the evenness index (J) between 0 - 0.739. Cluster analysis show that the highest similarity in the number of fish larvae between station 2 and stations 3, 2 and stations 4, stations 3 and 4. The highest similarity in the total number fish larvae was found between stations 1 and 5, according the Schoener index. The diet analysis was assessed using points and frequency of occurrence methods. No empty larval stomach were recorded during the study period and there is a high feeding activity and feeding intensity. The result of analyzing stomach contents indicated that the fish larvae were generalized feeders, consumed diatom, algae, detritus, copepoda, daphnia and eggs of molluscs. The larvae of C. carpio and C. auratus feed on diatom, while A. mossulensis, Aphanius sp. and T. ilisha on copepoda. The diversity index of gut content ranged form 1.731 for L. abu to 0.762 for C. carpio. The fish larvae differ in intensity of diet overlap depending on the nature of nutrition, and nine significance diet overlaps between larvae were detected. The significance overlaps ranged form 67.10% to 91.73 %. The highest diet overlap (91.73%) between C. carpio and C. auratus. A very strong diet overlap (101.22) between A. mossulensis and Aphanius sp.. No diet overlap were detected between the larvae of C. carpio, C. auratus, T. ilisha, Aphanius sp and A. mossulensis. The study showed the importance of the northern part of the Shatt Al - Arab River as a breeding and nursery ground for some fish

دراسة تاثير استهلاك منتوج علاجي متخمر مصنع من عزلة محلية من بكتيريا Lactobacillus salivarius في بعض المعايير الفسلجية في الجرذان المختبرية == Studying The Consuming Effect of Therapeutic Fermented Product Manufactured by Local Isolate Lactobacillus salivarius on Some Physiological Parameters in Experimental Rats

Author name: وسن جعفر ابراهيم
Supervisor name: غياث حميد مجيد | احمد عبود خليفة
General topic: Agricultural sciences
Specific topic: Plant Biotechnology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: عزلت ثمان عزلات محلية من بكتيريا L.salivarius من براز الاطفال الرضع بعمر (3 - 2) اشهر اعتمادا على الصفات المجهرية والفسلجية والكيموحيوية ، ثلاثة منها (LsP1 ، LsP2 ، LsP5) اظهرت فروقات معنوية (P < 0.01) في قابليتها على انتاج الحامض في الحليب مقارنة مع بقية العزلات والسلالة القياسية من بكتيريا L.acidophilus . اجريت غربلة للعزلات المحلية الثلاث (LsP1 ، LsP2 ، LsP5) لاختيار قابليتها على تحمل الظروف المشابهة لظروف القناة المعدية المعوية من حموضة وتركيز املاح الصفراء وتركيز الانزيمات الهاضمة ، حساسيتها تجاه تاثير بعض المضادات الحيوية واسعة الاستخدام في العلاج الطبيعي ، وقدرتها على تثبيط نمو بكتيريا E.coli O3 : H4 . اظهرت العزلة المحلية L.salivarius (LsP5) فروقات معنوية (P < 0.01) في تحملها لظروف القناة المعدية المعوية حيث كانت اعداد الخلايا الحية11×106 وبعد ثلاث ساعات من التعرض للعصير المشابه للعصارة المعدية انخفضت الى (105 x 35 cfu \ مل) عند رقم هيدروجيني 2 و(104 x 39 cfu \ مل) عند رقم هيدروجيني 1.5 . وفي العصير المشابه للعصارة المعوية كانت اعداد خلاياها الحية (109 x 39 cfu \ مل) عند تركيز املاح صفراء %1 و(108 x 18 cfu \ مل) عند تركيز املاح صفراء %2 ؛ واظهرت مقاومة عالية للتاثير المثبط لبعض مضادات الحياة حيث كان قطر الهالة الخالية من النمو عند المعاملة مع المضاد الحياتي (E) Erythromycin (1.91 سم) و(CTX) Cephotaxime (0.95 سم) و(CTP)Ciprofloxacin (1.23 سم) بينما لم يظهر اي تاثير مثبط لنموها عند المعاملة مع الـ (Am) Ampicilline وTrimethoprime + Sulfamethoxazol (SXT) . كما انها اظهرت تاثيرا مثبطا لنمو البكتيريا E.coli O3 : H4 حيث كان قطر الهالة الخالية من نمو البكتيريا المرضية (25 ملم) . درست قابلية هذه العزلات على مقاومة ظروف التصنيع والخزن المبرد لمدة اربعة اسابيع على درجة حرارة 4 م ، واظهرت العزلة LsP5 قدرة عالية على البقاء حية بعد انتهاء فترة الخزن حيث كانت اعداد الخلايا الحية (107 x 25.9 cfu \ مل) . اختيرت العزلة LsP5 لتصنيع منتوج لبني متخمر مشابه للحليب الاسيدوفيلي وذلك لدراسة تاثير استهلاك هذا المنتوج على اوزان ، وبعض المعايير الدموية (اعداد كريات الدم الحمراء RBC ، الهيموكلوبين Hb ، حجم الدم المضغوط PCV ، اعداد كريات الدم البيضاء WBC) والبيوكيمياوية (الكوليستيرول الكلي Tchol. ، البروتينات الدهنية واطئة الكثافة LDL ، البروتينات الدهنية عالية الكثافة HDL ، الكليسيريدات الثلاثية TG ، والبروتين الكلي TP) لذكور الجرذان المختبرية في تجربة اشتملت على 24 حيوانا ، قسمت الى ثلاث مجاميع (8 حيوانات لكل مجموعة) المجموعة الاولى (مجموعة السيطرة) تم تغذيتها بالعليقة الاعتيادية لمدة 30 يوم ، المجموعة الثانية (S) تم تغذيتها بالعليقة الاعتيادية بالاضافة الى 2 مل من الحليب الفرز المسترجع بنسبة %12 ، اما المجموعة الثالثة (P) تم تغذيتها بالعليقة الاعتيادية بالاضافة الى 2 مل من المنتوج المتخمر حيث اظهرت النتائج ما ياتي : - 1 - حصول زيادة معنوية (P < 0.01) في النسبة المئوية للزيادة الوزنية لحيوانات المجموعة (P) مقارنة مع حيوانات المجموعة (C) و(S) .2 - ارتفاع معنوي (P < 0.01) في معدلات كل من RBC ، Hb ، PCV ، وانخفاض معنوي (P < 0.01) في اعداد WBC في دم حيوانات مجموعة المعاملة (p) مقارنة مع حيوانات المجموعة (C) و(S) .3 - ظهر من دراسة العد التفريقي لكريات الدم البيضاء ان التجريع بالمنتوج المتخمر ادى الى ارتفاع معنوي (P < 0.01) في اعداد الخلايا اللمفاوية lymphcyctes ، وانخفاض معنوي (P < 0.01) في اعداد الخلايا العدلة Neutrophil بينما لم تظهر اي اختلافات معنوية في اعداد الـ Eosinophil والـ Basophil . 4 - انخفاض معنوي (P < 0.01) في قيم كل من Tchol. ، LDL ، TG وارتفاع معنوي (P < 0.01) في قيم الـ HDL وTP في مصل دم حيوانات المجموعة (P) مقارنة مع حيوانات المجموعة (C) و(S) . درست امكانية استخدام الحليب المتخمر في علاج ذكور الجرذان المختبرية المصابة ببكتيريا E.coli O3 : H4 في تجربة استندت على 24 حيوانا ، قسمت الى ست مجاميع (4 حيوانات لكل مجموعة) حيث تم تغذية جميع المجاميع بالعليقة الاعتيادية طيلة فترة التجربة البالغة ستة اسابيع بالاضافة الى المعاملات الاتية : - مجموعة السيطرة (C) بدون معاملة ، المجموعة الثانية (P) عوملت بجرعة 2 مل من الحليب المتخمر ، المجموعة الثالثة (E) جرعة بـ 2 مل من معلق الخلايا الحية لبكتيريا E.coli O3 : H4 ، المجموعة الرابعة (EP) جرعت بالبكتيريا المرضية لمدة ثلاثة اسابيع متتالية ثم جرعت بـ 2 مل من الحليب المتخمر لثلاثة اسابيع تالية ، المجموعة الخامسة (EM) جرعت بالبكتيريا المرضية لمدة ثلاثة اسابيع ثم جرعت بـ 2 مل من الميترونيدازول ، اما المجموعة السادسة (P + E) فجرعت بالمنتوج المتخمر لمدة ثلاثة اسابيع ثم بالمنتوج المتخمر و2 مل من معلق البكتيريا المرضية في وقت واحد لثلاثة اسابيع اخرى ؛ واظهرت النتائج ما ياتي : - 1 - ان اصابة الحيوانات بالبكتيريا المرضية E.coli O3 : H4 ادى الى انخفاض معنوي (P < 0.01) في اوزان الحيوانات كما نفقت بعض الحيوانات ، وكانت النسبة المئوية للحيوانات النافقة (% 55.5) .2 - ادى علاج الحيوانات بالحليب المتخمر الى زيادة معنوية في اوزان الحيوانات المعاملة (EP) مقارنة مع الحيوانات التي تم علاجها كيمياويا بالميترويندازول . كما ان التجريع المسبق بالمنتوج المتخمر ادى الى عدم ظهور فروقات معنوية في الزيادة الوزنية مقارنة مع حيوانات مجموعة السيطرة (C) ، كما لم تظهر اعراض الاصابة على الحيوانات بعد تعاطيها البكتيريا المرضية .3 - حصل انخفاض معنوي (P < 0.05) في قيمة كل من الهيموكلوبين وحجم الخلايا المضغوط وارتفاع معنوي (P < 0.05) في اعداد كريات الدم البيضاء في دم الحيوانات التي تم اصابتها بالبكتيريا المرضية وادى استخدام العلاج الحيوي بالحليب المتخمر الى اعادة قيم هذه المتغيرات الى حدودها الطبيعية مع وجود فروقات معنوية مقارنة مع استخدام الميترويندازول في علاج الحيوانات ، كما ان التجريع المسبق بالبكتيريا العلاجية ادى الى المحافظة على هذه القيم ضمن الحدود الطبيعية حتى بعد اصابتها بالبكتيريا المرضية .4 - انخفاض قيم البروتين الكلي (TP) بشكل معنوي (P < 0.05) في مصل الحيوانات المصابة خلال فترة المعاملة وادى العلاج الحيوي بالحليب المتخمر الى ارتفاع قيم البروتين الكلي بشكل معنوي (P < 0.05) مقارنة مع استخدام العلاج بالميترونيدازول ، كما لم يحصل انخفاض في قيم البروتين الكلي في مجموعة الحيوانات التي تم تجريعها مسبقا بالحليب المتخمر (E+P) .5 - لم تظهر النتائج اي تغيير (عدم وجود فروقات معنوية) في قيم البيليروبين الكلي (TB) لجميع المجاميع الحيوانية طيلة فترة التجربة . | To investigation the effect of Lactobacillus local isolate the present study was conducted . Eight local isolates have been isolated and identificated as Lactobacillus salivarius from infant stool samples aged 2 - 3 months depending on their microscopical , physiological and biochemical aspects . Three of these isolates (LsP1 , LsP2 , LsP5) revealed a significant differences in their ability of milky acid production . these isolates screened according to their ability to tolerance the environments like those in the Gastro - intestinal tract which include (high acidity , bile salts concentration , digestive enzyme concentration) , sensitivity to the effect of some widespread antibiotics and their ability to inhibit growth of E.coli O3 : H4 . The local isolate (LsP5) showed a significant differences in his tolerance to gastro - intestinal tract environments and the number of living cell after 3 hours of exposure to the juice like gastric juice is (35 x 105 cfu / ml) in pH 2 and (15 x 104 cfu / ml) in pH 1.5 and in the juice like intestinal juice the number of living cells is (39 x 109 cfu / ml) in % 1 bile salt concentration and in the concentration % 2 (18 x 108 cfu / ml) , and this isolate showed also higher resistance to the lethal effect of some antibiotics than the other isolates and the inhibition zone diameter in erythromycine (1.91 cm) , cephotaxime (0.95 cm) , ciproflexacine (1.23 cm) , whereas not appear any lethal effect when its treated with ampicilline and trimethoprime + sulfamethoxazol . It’s indicated higher inhibition effect on E.coli O3 : H4 and the inhibition growth zone is (25 mm) . In conclusion , the results revealed that LsP5 isolate has higher resistance to the fermented milk production and cold storage under 4˚C for four weeks than the other isolates . In view of the above results we chosed the Lactobacillus salivarius (LsP5) to production a fermented milk like acidophilus milk and the effect of oral administration to experimental mal rats on weight , some hematological and biochemical parameters were studied in an experiment include 24 animals divided into three groups (8 animals / group) first group (C) control had adopt fed , second group (S) had adopt fed plus 2 ml of reprepared skim milk (12 %) and the third group (P) had adopt fed plus 2 ml of fermented milk contain 109 cfu / ml of living cell of LsP5 isolate and the results showed that : - 1 - Significant increase (P < 0.01) in body weight in treated group (P) compare with group (C) and (S) .2 - Significant increase (P < 0.01) in the rates of red Blood Cell (RBC) , Hemoglobine (Hb) , Packed Cell Volume (PCV) and significant decrease (P < 0.01) in the number of White Blood Cell (WBC) in animals group (P) in comparison whit group (C) and (S) . 3 - The differential count of WBC showed that the oral administration of fermented milk (group P) caused Significant (P < 0.01) increase in the number of lymphocytes and decrease (P < 0.01) in nutrophil , while not indicate changes in eiosinophil and basophil number compare with group (C) and (S) .4 - Significant decrease (P < 0.01) in the rates of total cholesterol (Tchol.) , low density lipoproteins (LDL) , triglycerides (TG) and Significant increase (P < 0.01) in the rates of high density lipoproteins (HDL) and total protein (TP) in the serum of treated animals (group P) in comparison whit group (C) and (S) . The therapeutic effects of fermented product contains 109 cfu / ml of Lactobacillus salivarius (LsP5) living cell was conducted in an experiment include 24 male experimental rats divided into 6 group (4 animals / group) , all these animals had adopt fed during experimental time (6 weeks) in addition to the following daily treatments : - a. Group (C) with out any treatment .b. group (P) has been oral administration with 2 ml of fermented milk .c. group (E) has been infected by oral administration with 2 ml of suspension of E.coli O3 : H4 . d. group (EP) has been infected by disease bacteria for three weeks and then treated by oral administration with 2 ml of fermented milk .e. group (EM) has been infected by disease bacteria for three weeks and then treated by oral administration with chemical therapeutic metronidazol .f. The final group (P + E) has been oral administration with 2 ml of fermented milk for three weeks and then with fermented milk plus disease bacteria in the same time . The results may be summarized as follow : - 1 - Infection bacteria caused Significant (P < 0.01) decrease in animals body weight , Hb , PCV & TP and Significant (P < 0.01) increase in WBC in addition to some of animals die during the experimental time .2 - Animals treated with the fermented milk showed Significant increase (P < 0.01) in animals body weight in the otherwise the value of Hb ,PCV and WBC returned to the normal value in comparison whit animals treated with metronidazol .3 - Anon - Significant effects in the total bilirubine has been seen in all treated rats during the experimental time .
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